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Preprint ARTICLE | doi:10.20944/preprints202410.1788.v1

High-Performance Liquid Chromatography for the Determination of Cannabinoids in Commercial CBD Oil

Zehra Hajrulai-Musliu, Elizabeta Dimitrieska-Stojkovikj, Dimitar Gusheski, Dea Musliu, Daniel Velkovski

Subject: Medicine And Pharmacology, Pharmacy Keywords: CBD oil; cannabinoids; THC; HPLC technique

Online: 23 October 2024 (07:35:11 CEST)

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It is crucial to conduct quality control tests to assess the quality and consistency of medicinal plant products before they are used. Products can often be placed on the market without registration, but some countries may require manufacturers to present product labels to authorities before selling them. These labels should confirm the composition of the product, particularly the concentrations of cannabidiol (CBD). The information on the label should provide unambiguous details about the qualitative composition and concentration of primary cannabinoids, such as CBD and THC. However, since there is a lack of regulatory control, it is possible to find inaccurate information on the label. This study aimed to create and validate a high-performance liquid chromatography method for identifying active compounds (cannabinoids) in CBD oil. The main compounds identified included Cannabidivarin (CBDV), Cannabidiolic Acid (CBD-A), Cannabigerolic Acid (CBG-A), Cannabigerol (CBG), Cannabidiol (CBD), Tetrahydrocannabivarin (THCV), Cannabinol (CBN), ∆9- Tetrahydrocannabinol (d9 -THC) ∆8- Tetrahydrocannabinol (d8-THC), Cannabicyclol (CBL), Cannabichromene (CBC), and Tetrahydrocannabinolic Acid (THCA-A), determined in line with the International Conference on Harmonization's (ICH) guidelines. The method was validated for linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ). It was determined to be linear, with a correlation coefficient (R2) ranging from 0.99937 to 0.99973. The LOD and LOQ values calculated from the calibration curve ranged from 0.05 to 0.13 and 0.50 to 0.61 µg/mL, respectively. The method also exhibited acceptable precision, with relative standard deviation values lower than or equal to 2%. The method's accuracy was assessed through recovery percentages and fell within an acceptable range (98.12–102.39%). This study's rigorous methodology and comprehensive findings significantly contribute to cannabinoid analysis. This validated protocol was used to analyze the cannabinoid concentration levels in 14 CBD oil products in the Republic of North Macedonia. The performance parameters indicate that the developed and validated method is suitable for quantitatively determining cannabinoids in CBD oil. The analysis of 14 CBD oil products revealed no significant derogation regarding the labeling, i.e., they were by the producers' declared variability.

Preprint REVIEW | doi:10.20944/preprints202410.1622.v1

Advances in Encapsulating Marine Bioactive Compounds Using Nanostructured Lipid Carriers (NLC) and Solid Lipid Nanoparticles (SLN) for Health Applications

Rita Favas, Hugo Almeida, Andreia F. Peixoto, Domingos Carvalho Ferreira, Ana Catarina Silva

Subject: Medicine And Pharmacology, Pharmacy Keywords: healthcare; cosmetics; food supplements; marine bioactive compounds; lipid nanoparticles

Online: 22 October 2024 (02:47:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202410.1573.v1

Research Progress of Lactate and Its Regulated Small Molecule Drugs

Jin Liu, Feng Zhou, Yang Tan, Yang Tang, Lihui Li, Gang Pei, Ling Li

Subject: Medicine And Pharmacology, Pharmacy Keywords: Lactate; small molecules drugs; glycolysis; physiology and diseases

Online: 21 October 2024 (06:12:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1492.v1

Pegylated Carboxylic Graphene Oxide for Controlled Paclitaxel Delivery to Tumor Cells: In Vitro Evaluation and Cell Studies

Athina Angelopoulou, Myria Papachristodoulou, Efstathia Voulgari, Andreas Mouikis, Panagiota Zygouri, Dimitrios P Gournis, Konstantinos Avgoustakis

Subject: Medicine And Pharmacology, Pharmacy Keywords: graphene oxide; paclitaxel; pegylation; cytotoxicity; programmed cell death

Online: 18 October 2024 (14:18:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.0517.v1

Nanoparticles as Potential Delivery System of Phenothiazine Derivatives for Anticancer Application

Katarzyna Jelonek, Monika Musiał-Kulik, Małgorzata Pastusiak, Aleksander Foryś, Andrzej Zięba, Janusz Kasperczyk

Subject: Medicine And Pharmacology, Pharmacy Keywords: phenothiazine; phenothiazine derivatives; drug delivery system; nanoparticles; nanocarriers; anticancer drugs

Online: 8 October 2024 (08:56:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.2345.v1

In Situ Gel-Embedded Chitosan Nanoparticles for Nasal Delivery of Imipramine Hydrochloride: Optimization and Controlled Release Evaluation

Samer Adwan, Teiba Obeidi, Faisal Al-Akayleh

Subject: Medicine And Pharmacology, Pharmacy Keywords: Chitosan; Pluronic; Nanoparticles; In situ gel; Nasal drug delivery; Imipramine

Online: 30 September 2024 (10:43:28 CEST)

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Imipramine hydrochloride (IMP), a tricyclic antidepressant used for major depression, enuresis, and neuropathic pain, is limited by gastrointestinal complications, low oral bioavailability (44%), and complex dosing requirements. This study aimed to explore a novel non-invasive nasal delivery system using chitosan nanoparticles (Cs NPs) embedded in an in situ gel to address the limitations of oral IMP administration. Cs NPs loaded with IMP were synthesized via ionic gelation and assessed for precision in drug concentration using a validated HPLC method. The particles were integrated into a thermoresponsive polymer, Pluronic F127, to form an in situ gel suitable for nasal administration. The formulation was characterized for gelation temperature, duration, viscosity, mucoadhesive strength, and overall gel robustness. Drug release kinetics and the controlled release mechanism were studied using ex vivo permeation tests with Franz diffusion cells and nasal sheep mucosa. The optimized nanoparticle formulation (F4-50) exhibited a consistent PS of 141.7±2.2 nm, a ZP of 16.79±2.1 mV, and a high encapsulation efficiency of 67.71±1.9%. The selected in situ gel formulation, F4-50-P1, demonstrated a gelation temperature of 33.6±0.94°C and a rapid gelation time of 48.1±0.7 seconds. Transform-attenuated total reflectance infrared spectroscopy (ATR-IR) confirmed the compatibility and effective encapsulation of IMP within the formulation. The release profile of F4-50 included an initial burst release followed by a sustained release phase, with F4-50-P1 showing improved control over the burst release. The flux rates were 0.50±0.01 mg/cm²/h for F4-50 and 0.33±0.06 mg/cm²/h for F4-50-P1, indicating effective permeation. The developed chitosan nanoparticle-based in situ gel formulation provides a promising approach for the controlled release of IMP, enhancing therapeutic efficacy and patient compliance while mitigating the disadvantages associated with oral delivery.

Preprint REVIEW | doi:10.20944/preprints202409.2241.v1

Lignin: An Adaptable Molecule

Andreea Creteanu, Claudiu N. Lungu, Mirela Lungu

Subject: Medicine And Pharmacology, Pharmacy Keywords: lignin; polymers; drug molecules; biomolecules

Online: 30 September 2024 (07:35:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.2122.v1

What Do We Know about the Relationship between Lycopene and Metabolic Diseases? Review

Anna Kulawik, Judyta Cielecka-Piontek, Bogusław Czerny, Adam Kamiński, Przemysław Zalewski

Subject: Medicine And Pharmacology, Pharmacy Keywords: lycopene; metabolic syndrome; obesity; type 2 diabetes

Online: 26 September 2024 (12:13:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.2096.v1

Translational Proofs on Bee Pollen as a Source of Biopharmaceuticals for Neurodegeneration and Cancer Research: A Scoping Review and Prospective Reflections

Rachid Kacemi, Maria Graça Campos

Subject: Medicine And Pharmacology, Pharmacy Keywords: aging; apoptosis; autophagy; bee pollen; cancer; epigenetics; ferroptosis; human microbiota; inflammation; neurodegeneration; oxidative stress; polyphenols

Online: 26 September 2024 (09:56:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1939.v1

Enhanced Ocular Drug Delivery of Dexamethasone Using Chitosan-Coated Soluplus® Based Mixed Micellar System

Samer Adwan, Faisal Al-Akayleh, Madeha Qasmieh, Teiba Obeidi

Subject: Medicine And Pharmacology, Pharmacy Keywords: Soluplus®; Pluronic F-127; Dexamethasone; Mixed micelle; Chitosan coat

Online: 25 September 2024 (10:25:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1971.v1

Nanotechnological Advances in Burn Wound Care: Chitosan-Based Nanogel Incorporating Silver Sulfadiazine-loaded Nanosuspension for Partial Thickness Burns

Harshita Barkat, Md. Abul Barkat, Raisuddin Ali, Hazrina Ab Hadi

Subject: Medicine And Pharmacology, Pharmacy Keywords: nanosuspension; topical delivery; burn wound infection; nanogel; chitosan ;

Online: 25 September 2024 (05:24:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1541.v2

Advanced Delivery of Curcumin and Nicotinamide: Promising Solutions for Skin Infections and Melanoma

Omar Markab, Zainab Lafi, Naeem Shalan, Hala Al Daghistani, Ali Fahadawi, Razan Madi

Subject: Medicine And Pharmacology, Pharmacy Keywords: Natural compounds; melanoma; skin infection; synergism; liposomes

Online: 23 September 2024 (07:44:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1612.v1

Combinations of Terminalia bellirica (Gaertn.) Roxb. and Terminalia chebula Retz. Extracts with Selected Antibiotics Against Antibiotic-Resistant Bacteria: Bioactivity and Phytochemistry

Gagan Tiwana, Ian Edwin Cock, Matthew James Cheesman

Subject: Medicine And Pharmacology, Pharmacy Keywords: MRSA; ESBL; plant extracts; natural therapies; combinational therapies; metabolomics

Online: 20 September 2024 (11:27:47 CEST)

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Antimicrobial resistance (AMR) is a worsening global health crisis that has arisen due to antibiotic overuse and misuse. Antibiotic resistance renders standard treatments less effective, making it difficult to control some infections, thereby increasing morbidity and mortality. Medicinal plants are sustainable treatment options that are becoming increasingly important as antibiotics lose their efficacies. The present study evaluates the antibacterial activity of aqueous, methanolic and ethyl acetate extracts prepared using Terminalia bellirica and Terminalia chebula fruit against six bacterial pathogens using disc diffusion and broth microdilution assays. The aqueous and methanol extracts of T. bellirica and T. chebula showed substantial zones of inhibition (ZOIs) against S. aureus and MRSA. The activity against those bacteria was determined to be strong, with minimum inhibitory concentrations (MIC) ranging from 94 µg/mL to 392 µg/mL. Additionally, the T. bellirica methanolic extract showed noteworthy antibacterial activity against E. coli and an ESBL E. coli strain (MIC values of 755 µg/mL for both). The aqueous T. bellirica and T. chebula extracts also inhibited K. pneumoniae growth (MIC values of 784 µg/mL and 556 µg/mL respectively), whilst the methanolic extracts of these plants also inhibited ESBL K. pneumoniae growth (MIC values of 755 µg/mL and 1509 µg/mL respectively). Eighteen additive interactions were observed when extracts were combined with reference antibiotics. Notably, strong antagonistic interactions occurred when any of the extracts were mixed with polymyxin B. All extracts were subjected to LC-MS analysis, revealing several interesting flavonoids and tannins, including 6-galloylglucose, 1,2,6-trigalloyl-β-D-glucopyranose, 6-O-[(2E)-3-phenyl-2-propenoyl]-1-O-(3,4,5-trihydroxybenzoyl)-β-D-glucopyranose, propyl gallate, methyl gallate, sanguiin H4, hamamelitannin, pyrogallol, gallic acid, ellagic acid, chebulic acid, and chebuloside II. All extracts were nontoxic when tested in brine shrimp assays. This lack of toxicity, combined with their antibacterial activities suggests that these plant species may be promising sources of antibacterial compound(s) that warrant further study.

Preprint ARTICLE | doi:10.20944/preprints202409.1547.v1

Reducing Polypharmacy Through Team Resource Management in the Emergency Department: A Focus on Deprescribing in Elderly Patients

Hsiu-Wu Yang, Ching-Hsiang Yu, Teng-Yi Huang, Chia-Hui Huang, Yu-Jang Su

Subject: Medicine And Pharmacology, Pharmacy Keywords: comprehensive medication review; deprescribing; medication; polypharmacy

Online: 19 September 2024 (16:44:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1161.v1

The Efficacy and Safety of Oncolytic Viruses in Treatment of Glioma: A Meta-Analysis

Wen-Jing Zeng, Yi-Fan Zeng, Li-Jun Zhou, Jing Yi, Sheng Deng, Zheng-Zheng Liu, Qian-Qian Ye

Subject: Medicine And Pharmacology, Pharmacy Keywords: Glioma; Oncolytic viruses; Drug efficacy; Drug safety; Meta-analysis

Online: 17 September 2024 (05:01:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0976.v1

In Vitro Quality Assessment of Glibenclamide Tablets: Reference, Generic and Similar Drugs

Bruna Serio, Juliana Yzze, Suellen Rosa

Subject: Medicine And Pharmacology, Pharmacy Keywords: Glibenclamide; diabetes mellitus; quality control; drug; in vitro; pharmacopoeia

Online: 12 September 2024 (11:25:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0245.v1

Plasmid DNA Delivery into the Skin via Electroporation with a Depot-Type Electrode

Yuya Yoshida, Manami Aoki, Kalin Nagase, Koichi Marubashi, Hiroyuki Kojima, Shoko Itakura, Syuuhei Komatsu, Kenji Sugibayashi, Hiroaki Todo

Subject: Medicine And Pharmacology, Pharmacy Keywords: DNA vaccine; DNA delivery; electroporation; skin; dermal

Online: 3 September 2024 (18:05:27 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0137.v1

The Role of Peptides in Combatting HIV Infection: Applications and Insights

Naiera M Helmy, Keykavous Parang

Subject: Medicine And Pharmacology, Pharmacy Keywords: AIDS; Human Immunodeficiency Virus; Entry Inhibitors; Peptides; Vaccines

Online: 2 September 2024 (23:32:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.2211.v1

Phytochemical Analysis, Antimicrobial, Antibiofilm and Antioxidant Effects of a New Arctic Lichen Placidium Doesaiense Growing in Pakistan

Anja Manojlović, Abdul Nasir Khalid, Muhammad Usman, Olgica Stefanović, Nevena Đukić, Nedeljko Manojlović, Jovica Tomović

Subject: Medicine And Pharmacology, Pharmacy Keywords: Placidium doesaiense; Phytochemical Analysis; Antimicrobial; Antibiofilm; Antioxidant

Online: 30 August 2024 (09:38:31 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.2097.v1

An In-Depth Analysis of pH-Independent Controlled Drug Delivery Systems and Prospects for the Future

ANILKUMAR ADIMULAPU, Sabir Hussain, Rajeswari Saripilli, Rajesh Jesudasan

Subject: Medicine And Pharmacology, Pharmacy Keywords: Dosage form; Oral controlled delivery system; pH independent controlled drug delivery

Online: 28 August 2024 (19:44:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1533.v1

Nail Lacquer with Origanum vulgare Essential Oil and Biogenic Silver Nanoparticles for Onychomycosis: Development, Characterization, and Antifungal Effectiveness

Sara Scandorieiro, Natalia Rodrigues de Oliveira, Monique de Souza, Lidiane Vizioli de Castro-Hoshino, Mauro Luciano Baesso, Gerson Nakazato, Renata Katsuko Takayama Kobayashi, Luciano Aparecido Panagio, Audrey Alesandra Stinghen Garcia Lonni

Subject: Medicine And Pharmacology, Pharmacy Keywords: Nail mycosis; Antifungal nail lacquer; Topical therapy; Green nanotechnology; Oregano essential oil; Trichophyton spp.; Microsporum spp.

Online: 22 August 2024 (02:29:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1541.v1

Advanced Delivery of Curcumin and Nicotinamide: Promising Solutions for Skin Infections and Melanoma

Omar Markab, Zainab Lafi, Naeem Shalan, Hala Al Daghistani, Ali Fahadawi, Razan Madi

Subject: Medicine And Pharmacology, Pharmacy Keywords: Natural compounds; melanoma; skin infection; synergism; liposomes

Online: 21 August 2024 (11:40:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.0830.v1

Cost-Effectiveness Analysis of Pneumococcal Vaccine in the Pediatric Population: A Systematic Review

Nam Xuan Vo, Huong Lai Pham, Uyen My Bui, Han Tue Ho, Tien Thuy Bui

Subject: Medicine And Pharmacology, Pharmacy Keywords: Cost-effectiveness analysis; pneumococcal vaccine; pediatric; systematic review

Online: 12 August 2024 (23:52:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0309.v1

Compared Antileishmanial Activity of Clomiphene and Tamoxifen

Sergio Sifontes-Rodríguez, Alma Reyna Escalona-Montaño, Ricardo Mondragón-Flores, Niurka Mollineda Diogo, Lianet Monzote Fidalgo, Mónica Edith Mondragón-Castelán, Fedra Alardín Gutiérrez, Lourdes Araceli López-Enzana, Daniel Andrés Sánchez-Almaraz, Ofelia Pérez-Olvera, María Magdalena Aguirre-García

Subject: Medicine And Pharmacology, Pharmacy Keywords: Leishmania; clomiphene; tamoxifen; amastigote; promastigote; mouse

Online: 5 August 2024 (16:47:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0250.v1

Spectroscopic Investigation of Losartan and Glipizide Competitive Binding to Glycated Albumin in Polypharmacotherapy: A Comparative Study

Agnieszka Szkudlarek

Subject: Medicine And Pharmacology, Pharmacy Keywords: polypharmacotherapy; spectroscopic investigation; losartan; glipizide; glycated albumin; diabetes; AGEs; drug-albumin binding; drug-drug interactions

Online: 5 August 2024 (08:19:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0184.v1

Effect of Temperature and Molarity on the Evaluation of Antimicrobial, Cytotoxic, and Antioxidant Activities of the Bio-Oil from Açaí Seed (Euterpe oleracea, Mart.)

Iago Castro da Silva, Pamela Suelen da Silva Seabra, Kely Campos Navegantes Lima, Ricardo Barbosa Bezerra Filho, Alanna Lorena P. dos Santos, Amanda Caroline dos Santos Monteiro, Giovanna Quintero Pamplona, Alexandre Guilherme da Silva Dias, Rayane Caroline dos Santos Pereira, Leticia Araujo Costa, Thays Jhessica Mota Pinheiro, Lauro Henrique Hamoy Guerreiro, Nélio Teixeira Machado, Marta Chagas Monteiro

Subject: Medicine And Pharmacology, Pharmacy Keywords: Açaí; Bio-oil; Pyrolysis

Online: 2 August 2024 (16:44:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0165.v1

The Impact of Home Medication Management Practices on Medication Adherence

Lisa Gualtieri, Meredith Steinfeldt, Eden Shaveet, Brandon Estime, Meera Singhal

Subject: Medicine And Pharmacology, Pharmacy Keywords: medication adherence; home medication management; medication storage

Online: 2 August 2024 (10:51:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.0034.v1

A Review of Studies on HIV Pre-exposure prophylaxis in Community Pharmacies in States with Restrictive Pharmacist Prescription Authority in the United States

Hongmei Wang, Dominique Guinn, Xavier Roshitha Ramisetty, Thomas P Giordano, Ivy O Poon

Subject: Medicine And Pharmacology, Pharmacy Keywords: HIV; preexposure prophylaxis medications; community pharmacy; pharmacy service

Online: 1 August 2024 (15:37:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.0020.v1

The Current Principles Define the Isolation of Health-Promoting Substances from Secondary Herbal Materials

Valdas Jakštas

Subject: Medicine And Pharmacology, Pharmacy Keywords: refinery; sustainability; fruit cake; seeds; phenolics; anthocyanins; phytopharmaceuticals

Online: 1 August 2024 (12:36:52 CEST)

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Agricultural waste is rich in bioactive molecules. In evaluating the viability of circular models for the development of health-promotional substances and final products, it is important to highlight that the processing industry of fruits and other valuable herbal materials generates a considerable number of by-products and waste containing health-promoting components. These by-products can be utilized purposefully used in pharmaceuticals and related areas for the development of health promotional products. The linear utilization of agricultural waste results in the loss of a range of valuable bioactive compounds, including polyphenols (anthocyanins, flavonoids, phe-nolic acids, and related compounds), antioxidants from other groups, as well as phytosterols, tocopherols and fatty acids. As an illustrative example, the waste materials of species belonging to the Vaccinium genus represent a notable secondary resource that can be purposefully applied to the development of health-promoting substances. The fruits of these wasted herbal materials have been found to contain reliable polyphenols, which play a pivotal role in the prevention of various chronic conditions, including precancerous conditions, inflammatory diseases and other ailments. In addition, the fruits of blackberries, elderberries, and purple corn which are similarly rich in anthocyanins also provide a promising avenue for further developments. Phenolic compounds suitable for recycling are also found in sugarcane harvesting by-products. Tomato waste contains a significant amount of the valuable carotenoid lycopene. The additional value of the above-mentioned fruit processing by-products may attribute to specified physiological functions, which, if used properly, can contribute to the prevention of certain diseases, and improving of quality of life. This review assessed the scope and gaps in the existing principles on the isolation of health-promoting substances from herbal secondary materials.

Preprint ARTICLE | doi:10.20944/preprints202407.2499.v1

Understanding Physiciansʹ Journeys with Corticosteroids – Experiences, Knowledge, and the Shadows of Fears

Muna Barakat, Rana Abu Farha, Samar Thiab, Nesreen A. Salim, Anas O. Alshweiki, Diana Malaeb, Malik Sallam, Roa’a Thaher

Subject: Medicine And Pharmacology, Pharmacy Keywords: physicians; corticosteroids; experiences; knowledge; fears

Online: 31 July 2024 (11:42:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2394.v1

Vernonia amygdalina Leaf Extract Induces Apoptosis in HeLa Cells: A Metabolomics and Proteomics Study

Pawitrabhorn Samutrtai, Yodying Yingchutrakul, Kriangsak Faikhruea, Tirayut Vilaivan, Vorrapon Chaikeeratisak, Jaruwan Chatwichien, Sucheewin Krobthong, Chanat Aonbangkhen

Subject: Medicine And Pharmacology, Pharmacy Keywords: medicinal plants; metabolomics; proteomics; cancer; apoptosis; JNK; p53; caspase-9; PTEN; XBP1

Online: 30 July 2024 (10:24:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2268.v1

Automated Production of [⁶⁸Ga]Ga-desferrioxamine B on Two Different Synthesis Platforms

Martin Kraihammer, Miloš Petřík, Christine Rangger, Michael Gabriel, Hubertus Haas, Bernhard Nilica, Irene Virgolini, Clemens Decristoforo

Subject: Medicine And Pharmacology, Pharmacy Keywords: desferrioxamine B; gallium-68; PET; infection; imaging; validation

Online: 29 July 2024 (08:43:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2145.v1

An Internationally-Derived Process of Healthcare Professionals’ Proactive Deprescribing Steps and Constituent Activities

Sion Scott, Natalie Buac, Debi Bhattacharya

Subject: Medicine And Pharmacology, Pharmacy Keywords: polypharmacy; shared decision making; behaviour change; intervention; deprescription

Online: 26 July 2024 (12:48:17 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.0471.v2

Advances in Modeling Approaches for Oral Drug Delivery: Artificial Intelligence, Physiologically Based Pharmacokinetic, and First-Principle Models

Yehuda Arav

Subject: Medicine And Pharmacology, Pharmacy Keywords: Mathematical models; Artificial intelligence; Machine learning; deep learning; QSAR; PBPK; CFD; Molecular dynamics

Online: 15 July 2024 (15:36:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0810.v1

Development of a Metaverse Art Gallery of Image Chronicles (MAGIC) for Healthcare Education: A Digital Health Humanities Approach to Patients’ Medication Experiences

Kevin Yi-Lwern Yap, Jayen Ho, Phylaine Shu-Ting Toh

Subject: Medicine And Pharmacology, Pharmacy Keywords: Art Therapy; Hero-Villain Portraits; Medical Humanities; Patient Medication Experiences; Metaverse Art Gallery; Pharmacy Education; Digital Health Humanities; Generative Artificial Intelligence

Online: 10 July 2024 (10:12:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0824.v1

Hydrogen Bonding in Amorphous Indomethacin

Chris J Benmore, Jeffrey L. Yarger, Stephen Davidowski, Cyd Shrader, Pamela Smith, Stephen R Byrn

Subject: Medicine And Pharmacology, Pharmacy Keywords: Amorphous; Pair Distribution Function; Indomethacin; X-ray diffraction; Monte Carlo simulation

Online: 10 July 2024 (05:08:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202405.2107.v2

Harnessing Biopolymer Gels for Theranostic Applications: Imaging Agent Integration and Real-Time Monitoring of Drug Delivery

Pranita S Jirvankar, Surendra S Agrawal, Nikhita Chambhare, Rishabh Agrawal

Subject: Medicine And Pharmacology, Pharmacy Keywords: Biopolymer gel; Real-Time monitoring; Imaging agent

Online: 9 July 2024 (12:29:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0542.v1

Development of an HPLC Method through the QbD Approach for the Quantification of Honokiol and Metformin Hydrochloride – Method Validation and Pharmaceutical Applications on Oral Dosage Forms

Mousa Sha’at, Adrian Florin Spac, Iulian Stoleriu, Alexandra Bujor, Monica Stamate Cretan, Mihaela Hartan, Lăcrămioara Ochiuz

Subject: Medicine And Pharmacology, Pharmacy Keywords: QbD optimization; HPLC; validation; greenness; assay; dissolution test; metformin hydrochloride; honokiol; oral dosage forms; diabetes mellitus.

Online: 9 July 2024 (06:08:04 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.0579.v1

Understanding USP Chapters 661 and 671: A Comprehensive Experiments, Review and Implementation Guide

KANNAN JAKKAN, Narasimha Naidu Mopidevi

Subject: Medicine And Pharmacology, Pharmacy Keywords: USP Chapters 661; 671; Pharmaceutical packaging; Container quality; Performance testing; Regulatory compliance

Online: 8 July 2024 (08:32:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0905.v1

Enhanced Skin Penetration of Ascorbyl Palmitate Achieved via Liposomal Encapsulation: Tape Stripping Evaluation

Aleksandra Stolić Jovanović, Vanja Tadic, Milica Martinović, Ana Žugić, Ivana Nešić, Stevan Blagojević, Nebojša Jasnić, Tomislav Tosti

Subject: Medicine And Pharmacology, Pharmacy Keywords: ascorbyl palmitate; liposomes; cream; emulgel; tape stripping

Online: 13 June 2024 (09:41:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0786.v1

Pharmaco-Epidemiological Study and Correlation between Antibiotic Resistance and Antibiotic Consumption in a Tunisian Teaching Hospital from 2010 to 2022

Yosr Kasbi, Fatma Sellami, Asma Ferjani, Aimen Abbassi, Ilhem Boutiba Ben Boubaker

Subject: Medicine And Pharmacology, Pharmacy Keywords: Tunisian hospital; antibiotics; antimicrobial resistance; AWaRe classification; correlation; Consumption

Online: 12 June 2024 (14:12:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0471.v1

Advances in Modeling Approaches for Oral Drug Delivery: Artificial Intelligence, Physiologically Based Pharmacokinetic, and First-Principal Models

Yehuda Arav

Subject: Medicine And Pharmacology, Pharmacy Keywords: Mathematical models; Artificial intelligence; Machine learning; deep learning; QSAR; PBPK; CFD; Molecular dynamics

Online: 10 June 2024 (04:01:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.0286.v1

A Novel Method for the Preparation of Casein-Fucoidan Compo-Site Nanostructures

Bissera Pilicheva, Nikolay Zahariev

Subject: Medicine And Pharmacology, Pharmacy Keywords: casein; fucoidan; polyelectrolyte complexation; spray drying; experimental design; nanocomposites

Online: 5 June 2024 (14:56:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0584.v2

Characteristic Metabolites and Antioxidant Activities of Essential Oils from Phlomoides rotata (Benth. ex Hook. f.) Mathiesen

Zheng Pan, Chen Xie, Jie Luo, Xiao Tong Yan, Yong Mei Su, Jian Wang, Nasir Mehmood Khan, Shujaat Ahmad

Subject: Medicine And Pharmacology, Pharmacy Keywords: Phlomoides rotata ; (Benth. ex Hook. f.) Mathiesen (Lamiophlomis rotata ; (Benth.) Kudô; ); essential oils; ; characteristic metabolites; ; metabolomics; fatty acids; apocarotenoids ; (norisoprenoids); biosynthesis; antioxidant activities; pro-oxidant activities.

Online: 3 June 2024 (11:59:55 CEST)

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Limited research has been conducted on the metabolites present in the essential oils (EOs) of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen (PR, syn. Lamiophlomis rotata (Benth.) Kudô), and their potential pharmacological activities, such as antioxidant properties, are often overlooked. We conducted a metabolomics-based screening to identify the characteristic metabolites (CMs) in the EOs extracted from PR. Meanwhile, we evaluated their in vitro antioxidant activities (AOAs). The EOs obtained through hydro-distillation are characterized by a light yellow color and a fresh, elegant fragrance. Cryoprecipitation was used to separate the crystals (Crs) from the EOs, resulting in the crystal-free EOs (CFs). A total of 121 components are identified and quantified in the EOs, Crs, and CFs. Among them, 84 were reported for the first time from such EOs. The major compounds include long-chain fatty acids (LCFAs) and their esters, such as palmitic acid (PA), myristic acid (MA), linoleic acid (LA), oleic acid (OA), stearic acid, and methyl palmitate (MP). Furthermore, 11 kinds of apocarotenoids (also called norisoprenoids) were first systematically investigated. Among them, eight C13-norisoprenoids, with trans-β-damascenone (DN) being particularly notable for its flavor, were identified. A sum of 11 CMs are screened , including PA, MA, LA, OA, MP, hexahydrofarnesyl acetone, phytol (PT), DN, stearic acid, linalool, and hexanal. These CMs are biosynthesized through FAs and 2-C-Methyl-D-erythritol 4-phosphate/1-Deoxy-D-xylulose 5-phosphate pathways. The in vitro AOAs of EOs, Crs, CFs, and seven selected CMs (PA, MA, LA, OA, MP, PT, and DN) were evaluated. Generally, PA and MA exhibited pro-oxidant activities (POAs) or weak AOAs. LA and OA demonstrated POAs or weak AOAs at lower concentrations, but at higher concentrations, they displayed medium to strong AOAs. Importantly, the CFs typically exhibited stronger AOAs compared to the corresponding EOs and Crs, potentially due to variations in their PA content. Overall, this study provides valuable insights into the potential utilization of EOs from PR.

Preprint REVIEW | doi:10.20944/preprints202405.2107.v1

Harnessing Biopolymer Gels for Theranostic Applications: Imaging Agent Integration and Real-Time Monitoring of Drug Delivery

Pranita S Jirvankar, Surendra S Agrawal, Nikhita Chambhare, Rishabh Agrawal

Subject: Medicine And Pharmacology, Pharmacy Keywords: Biopolymer gel; Real-Time monitoring; Imaging agent

Online: 31 May 2024 (09:11:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1494.v1

A Comparative Analysis of the Phytochemical Profiles of Lonicera japonica Thunb. Flos Thunb. Species Native to the Mountainous and Coastal Regions of Romania

Nadia Dăineanu, Sebastian Mihai, Iuliana Stoicescu, Denisa Elena Dumitrescu, Antoanela Popescu, Cerasela Elena Gîrd

Subject: Medicine And Pharmacology, Pharmacy Keywords: Lonicera japonica Thunb.; antioxidant; LC/MS; GC/MS

Online: 23 May 2024 (10:45:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1323.v1

Evaluation of Photodynamic Therapy as a New Therapeutic Approach Using Quinizarin-Loaded Nanocapsules: In Vitro Cytotoxicity, Permeation in 3D Bioprinted Skin Equivalent and In Vitro Cytokines Modulation

Stéphanie Rochetti do Amaral, Camila Fernanda Amantino, Aleksandar Atanasov, Stefanie Souza, Richard Moakes, Sonia M Oliani, Liam M Grover, Fernando Lucas Primo

Subject: Medicine And Pharmacology, Pharmacy Keywords: Drug Delivery; Nanotechnology; Skin Inflammation; Photodynamics

Online: 21 May 2024 (17:22:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.1349.v1

Potentially Inappropriate Medication: A Pilot Study in Institutionalized Older Adults

Amanda Andrade, Tânia Nascimento, Catarina Cabrita, Helena Leitão, Ezequiel Pinto

Subject: Medicine And Pharmacology, Pharmacy Keywords: drug interactions; institutionalized elderly; medication review; polypharmacy; potentially inappropriate medications

Online: 21 May 2024 (05:43:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0709.v1

Potential Impact of Metabolic Syndrome Control on Cardiovascular Risk in Elderly Patients with Diabetes: A Cross-Sectional Study

Tânia Nascimento, Margarida Espírito-Santo, Adriana Gonçalves, Ezequiel Pinto, Ana Luísa De Sousa-Coelho, Maria Dulce Estêvão

Subject: Medicine And Pharmacology, Pharmacy Keywords: cardiovascular risk; ADVANCE risk score calculator; metabolic syndrome; type 2 diabetes mellitus; elderly

Online: 10 May 2024 (16:57:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0516.v1

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed by TLC and HPTLC

Katarzyna Bober-Majnusz, Alina Pyka-Pająk

Subject: Medicine And Pharmacology, Pharmacy Keywords: caffeine; propyphenazone; paracetamol; TLC-densitometry; limit of detection

Online: 8 May 2024 (15:14:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0419.v1

Chemical Content and Cytotoxic Activity on Various Cancer Cell Lines of Chaga (Inonotus obliquus) Growing on Betula pendula and Betula pubescens

Ain Raal, Hedi Kaldmäe, Karin Kütt, Katrin Jürimaa, Maidu Silm, Uko Bleive, Alar Aluvee, Kalev Adamson, Marili Vester, Mart Erik, Oleh Koshovyi, Khan Viet Nguyen, Hoai Thi Nguyen, Rein Drenkhan

Subject: Medicine And Pharmacology, Pharmacy Keywords: Betula pendula; Betula pubescens; birch; cancer, cell lines; chaga; Estonia; glucans; Inonotus obliquus; triterpenoids

Online: 8 May 2024 (09:48:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0380.v1

The Investigation of the Synthesis, Characterization, Molecular Docking, and Pharmacokinetic Properties of New Thiocarbohydrazones Based on Schiff Bases

Nalan Turkoz Karakullukcu

Subject: Medicine And Pharmacology, Pharmacy Keywords: thiocarbohydrazones; Schiff base; moleculer docking; urease inhibition; pharmacokinetic properties; drug combinations ;

Online: 8 May 2024 (02:40:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0123.v1

Co-amorphization of Acemetacin with Basic Amino Acids as Co-formers for Solubility Improvement and Side Effect Mitigation

Jiayue Hou, Peixu Zhao, Yanfei Wang, Xiwei Jiang, Qiang Fu

Subject: Medicine And Pharmacology, Pharmacy Keywords: acemetacin; co-amorphous; basic amino acids; gastric ulcer

Online: 7 May 2024 (03:15:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1989.v1

Effect of Modulator Therapies on Nutritional Risk Index in Adults with Cystic Fibrosis: A Prospective Cohort Study

Nadir Yalcin, Esen Deniz Akman, Oğuz Karcıoğlu, Karel Allegaert, Kutay Demirkan, Ebru Damadoglu, Ali Fuat Kalyoncu

Subject: Medicine And Pharmacology, Pharmacy Keywords: cystic fibrosis; adults; nutritional risk index; nutritional therapy; modulators; rare disease; orphan disease

Online: 30 April 2024 (14:33:36 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.1967.v1

Pharmacovigilance Strategies to Address Resistance to Antibiotics and Inappropriate Use—A Narrative Review

Valcieny Sandes, Albert Figueras, Elisangela Costa Lima

Subject: Medicine And Pharmacology, Pharmacy Keywords: pharmacovigilance; antibiotic use; inappropriate use; antimicrobial resistance; antimicrobial stewardship

Online: 30 April 2024 (07:58:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1879.v1

Taurine Neuroprotection and Neurogenesis Effect in Chronic Ethanol Induced Rats

Patricia Rodella, Diogo Boreski, Luiz Fernando Takase, Edmo Atique Gabriel, Marcus Alexandre Mendes Luz, Chung Man Chin

Subject: Medicine And Pharmacology, Pharmacy Keywords: taurine; neuroprotection; neurogenesis; hipocampus; ethanol consumption

Online: 29 April 2024 (10:34:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1772.v1

Development and Characterization of a Microemulsion Con-taining a Cannabidiol Oil and a Hydrophilic Extract from Sambucus ebulus for Topical Administration

Teresa Areses-Huete, Damian Cordoba-Diaz, Ana Isabel Torres-Suárez, Manuel Cordoba-Diaz

Subject: Medicine And Pharmacology, Pharmacy Keywords: cannabidiol; microemulsion; phyto-cannabinoids; rheological properties; diffusion studies; vegetal extract

Online: 26 April 2024 (17:00:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.1013.v3

Computational Pharmaceutics: A Comprehensive Review of Past Achievements, Present Applications, and Future Challenges

Yogesh Chaudhari, Manisha Chaudhari, Abhijeet Puri, Mahesh Nemade

Subject: Medicine And Pharmacology, Pharmacy Keywords: computational pharmaceutics; AI; multi-scale modeling; quantum computing; drug discovery; blockchain frameworks

Online: 22 April 2024 (18:38:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1330.v1

Isoquinoline Alkaloids Interaction With Plasma Proteins

Aleksandra Marciniak, Aleksandra Kotynia, Edward Krzyżak, Żaneta Czyżnikowska, Sylwia Zielińska, Weronika Kozłowska, Marcel Białas, Adam Matkowski, Anna Jezierska-Domaradzka

Subject: Medicine And Pharmacology, Pharmacy Keywords: protopine; allocryptopine; albumin; orosomucoid; spectroscopy

Online: 19 April 2024 (10:26:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.1013.v2

Computational Pharmaceutics: A Comprehensive Review of Past Achievements, Present Applications, and Future Challenges

Yogesh Chaudhari, Manisha Chaudhari, Abhijeet Puri, Mahesh Nemade

Subject: Medicine And Pharmacology, Pharmacy Keywords: computational pharmaceutics; AI; multi-scale modeling; quantum computing; drug discovery; blockchain frameworks

Online: 19 April 2024 (08:01:11 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.1013.v1

Computational Pharmaceutics: A Comprehensive Review of Past Achievements, Present Applications, and Future Challenges

Yogesh Chaudhari, Manisha Chaudhari, Abhijeet Puri, Mahesh Nemade

Subject: Medicine And Pharmacology, Pharmacy Keywords: Computational Pharmaceutics, AI, multi-scale modeling, quantum computing, drug
discovery, blockchain frameworks ;

Online: 16 April 2024 (10:32:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0755.v1

NegEnt Spray: An Original CE Medical Device, Based on Liposomal Cannabidiol and Chamomile Hydrolate, for the Treatment of Muscles and Joints

Tullio Scrimali

Subject: Medicine And Pharmacology, Pharmacy Keywords: ; Musculoskeletal disorders, Cannabidiol, Liposomal technology, Chamomile hydrolate, Topical treatment.

Online: 11 April 2024 (09:02:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0735.v1

Preparation and Evaluation of Niosomal Formulation for Solubility Enhancement of Anti-fungal Agent for the Treatment of Oral Candidiasis

Yogesh Chaudhari, Manisha Chaudhari, Shikha Prasad

Subject: Medicine And Pharmacology, Pharmacy Keywords: Candida albicans; Oral thrush; Anti-fungal; Subgingival film; Niosomes

Online: 11 April 2024 (04:02:09 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202404.0714.v1

Pharmacists’ Behavioral Change after Attending a Palliative Care Education Program in a Multi-Prefecture

Masahiro Yamada, Mayako Uchida, Masao Hada, Haruka Wakabaashi, Daigo Inma, Shunji Ariyoshi, Hidetoshi Kamimura, Tohru Haraguchi

Subject: Medicine And Pharmacology, Pharmacy Keywords: pharmacists’ behavioral change; cancer; palliative care; education program; multi-prefecture; transtheoretical model

Online: 10 April 2024 (08:20:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1760.v1

Real-World Effectiveness, Persistence, Adherence, and Safety of Janus Kinase Inhibitor Baricitinib in Rheumatoid Arthritis: A Long-Term Study

Alberto Calvo-Garcia, Esther Ramírez Herráiz, Irene Maria Llorente Cubas, Blanca Varas De Dios, Alberto Morell Baladrón, Juana Benedí González, Rosario García-Vicuña

Subject: Medicine And Pharmacology, Pharmacy Keywords: JAK-inhibitor; baricitinib; rheumatoid arthritis; real-word data; persistence; adherence; safety; unmet needs.

Online: 28 March 2024 (10:30:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1287.v1

In vitro Antibacterial Activity of Mauritia flexuosa and Carapa guianensis Oils and Menta piperita and Eugenia caryophyllus Essential Oils Added to Ozone

Fernando Antonio Madeira Marinho, Ana Flávia Machado De Carvalho, Hebert Lima Batista, Edenilson Dos Santos Niculau, Bruno Leonardo Almeida Viana, Natália Ferreira Almeida

Subject: Medicine And Pharmacology, Pharmacy Keywords: Antibacterial activity; Chemical analysis; Essential and fixed oils; Ozonation

Online: 21 March 2024 (14:02:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0811.v1

Development of New Drugs to Treat Tuberculosis Based on the Dinitrobenzamide Scaffold

Tiago Delgado, João Pais, David Pires, Filipe Estrada, Rita Guedes, Elsa Anes, Luís Constantino

Subject: Medicine And Pharmacology, Pharmacy Keywords: Tuberculosis; DprE1; DNB; TB; Nitrobenzamides

Online: 14 March 2024 (11:12:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0740.v1

Automated Non-Sterile Pharmacy Compounding: A Multi-Site Study in European Hospital and Community Pharmacies with Pediatric Immediate Release Propranolol Hydrochloride Tablets

Niklas Sandler, Farnaz Shokraneh, Mahsa Bahman, Julius Lahtinen, Niko Hassinen, Sari Airaksinen, Soumya Verma, Ludmila Hrizanovska, Jana Lass, Jyrki Heinämäki, Urve Paaver, Anssi Karlstedt, Janika Tähnas, Christoph Klaas, Catharina Kern, Adeline Bienfait, Frédéric Lagarce, Dominic Fenske, Katrin Muehlenfeld, Julia Malik, Holger Scherliess, Samuli Ojala, Sara P Cruz, Mattias Paulsson, Katja Kammonen, Maria Rautamo, Hendrik Lueck, Herwig Heindl, Laurent Carrez, Antoine Pierrot, Ralf Harnish, Stephanie Stareprawo, Marija Tubic-Grozdanii, Stefanie Zibolka, Uli Loesch, Martina Jeske, Ulrich Griesser, Karin Hummer, Andreas Thalmeier, Marianne Leitner, Susan Bischoff, Ralph Heimke-Brinck, Janine Paulus-Rohmer, Karim Khouckh, Fabien Bruno, Anna Harjans, Torsten Hoppe-Tichy

Subject: Medicine And Pharmacology, Pharmacy Keywords: automated compounding; hospital and community pharmcies; pharm inks; integrated quality control

Online: 13 March 2024 (12:02:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1722.v1

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Alina Pyka-Pająk

Subject: Medicine And Pharmacology, Pharmacy Keywords: metronidazole; tinidazole; pharmaceutical preparation; TLC; densitometry

Online: 29 February 2024 (13:07:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1672.v1

FT-IR Analysis and Acute Toxicity of the Extracts of Wild-Growing Saussurea sordida, Saussurea alpina Native to Kazakhstan

Kulpan Orynbassarova, Aishabibi Karzhaubayeva, Saule Ordabayeva, Aigul Serikbayeva, Raushan Kozykeyeva, Zhanar Shimirova, Kairat Zhakipbekov, Kozykeyeva Raikhan, Elmira Kartbayeva, Aknur Turgumbayeva

Subject: Medicine And Pharmacology, Pharmacy Keywords: Saussurea L.; biologically active substances; acute toxicity; histological study

Online: 29 February 2024 (11:40:56 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.1496.v1

Proton-Pump Inhibitors in Eosinophilic Esophagitis: A Review Focused on the Role of Pharmacogenetics

Leticia Rodríguez-Alcolado, Pilar Navarro, Laura Arias-González, Elena Grueso-Navarro, Alfredo J Lucendo, Emilio J Laserna-Mendieta

Subject: Medicine And Pharmacology, Pharmacy Keywords: Proton-pump inhibitors; eosinophilic esophagitis; pharmacogenetics; CYP219 gene; STAT6 gene.

Online: 26 February 2024 (23:53:49 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.1300.v1

Enhancing Cancer Therapy through Polymeric Nano-particles: Targeted Drug Delivery Strategies

Desta Tesfay Mezgebo, Yohannes Reda Tesfay, Guruduta Pattnaik

Subject: Medicine And Pharmacology, Pharmacy Keywords: Polymeric Nanoparticle; Drug Delivery; Cancer; Ligands; Antibodies

Online: 22 February 2024 (12:20:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0115.v3

Antibacterial Activity and Mechanisms of Plant Flavonoids against Gram-Negative Bacteria Based on the Antibacterial Statistical Model

Yu Yan, Xuexue Xia, Aiman Fatima, Li Zhang, Ganjun Yuan, Fengxian Lian, Yu Wang

Subject: Medicine And Pharmacology, Pharmacy Keywords: Action mode; Antimicrobial; Escherichia coli; Flavonoids; Gram-negative bacteria; Gram-positive bacteria; Lipophilicity; LogP; MIC; Statistical model

Online: 20 February 2024 (10:48:13 CET)

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The antimicrobial quantitative structure–activity relationship of plant flavonoids against Gram-positive bacteria was established in our previous works, and the cell membrane was confirmed as a major site of action. To investigate whether plant flavonoids have similar antibacterial effects and mechanisms against both Gram-negative and Gram-positive bacteria, here, the minimum inhibitory concentrations (MICs) of 37 plant flavonoids against Escherichia coli were determined using the microdilution broth method, and then the correlation between their lipophilic parameter ACD/LogP or LogD_7.40 value and their MIC was analyzed. Simultaneously, the correlation between the ACD/LogP or LogD_7.40 value and the MIC of 46 plant flavonoids reported in literature against E. coli was also analyzed. Both sets of results showed that there is a significant correlation between the LogP value and the MIC of plant flavonoids against Gram-negative bacteria. However, it is difficult to effectively predict the MIC of plant flavonoids against Gram-negative bacteria from their lipophilic parameters. By comparing two regression curves derived from plant flavonoids against Gram-negative and Gram-positive bacteria, it was further discovered that the antibacterial activities of most plant flavonoids against Gram-negative bacteria are stronger than those against Gram-positive bacteria when their LogP values are less than about 3.0, but the opposite is true when their LogP values are more than about 3.6. Moreover, this comparison also suggests that unlike mainly acting on the cell membrane of Gram-positive bacteria, plant flavonoids have multiple mechanisms against Gram-negative species, while the cell membrane is also an important action site among them. Combined with the correlation analyses between the enzyme inhibitory activity and the LogP value of the reported flavonoids, it was further suggested that DNA gyrase is another important target of plant flavonoids against Gram-negative bacteria.

Preprint REVIEW | doi:10.20944/preprints202402.0964.v1

Chemistry and Pharmacology of Delta-8-Tetrahydrocannabinol

Maged S. Abdel-Kader, Mohamed M. Radwan, Ahmed M. Metwaly, Ibrahim H. Eissa, Arno Hazekamp, Mahmoud A. ElSohly

Subject: Medicine And Pharmacology, Pharmacy Keywords: Δ 8-THC; isolation; chemistry; analysis; pharmacology

Online: 19 February 2024 (11:49:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1033.v1

The Eucalypt Extracts Prepared by a Non-wasting Method and Their 3D-Printed Dosage Forms With an Antimicrobial and Anti-inflammatory Activity

Oleh Koshovyi, Mykola Komisarenko, Tatyana Osolodchenko, Andrey Komissarenko, Reet Mändar, Siiri Kõljalg, Jyrki Heinämäki, Ain Raal

Subject: Medicine And Pharmacology, Pharmacy Keywords: Eucalypt leaves extract; complex processing; phenolics; terpenes; polyethylene oxide; aqueous gel; semi-solid extrusion 3D printing

Online: 19 February 2024 (10:17:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0584.v1

Characteristic Metabolites and Antioxidant Activities of Essential Oils from Phlomoides rotata (Benth. ex Hook. f.) Mathiesen

Zheng Pan, Chen Xie, Jie Luo, Xiao Tong Yan, Yong Mei Su, Anwar Ul Haq, Nasir Mehmood Khan, Shujaat Ahmad, Jian Wang

Subject: Medicine And Pharmacology, Pharmacy Keywords: Phlomoides rotata (Benth. ex Hook. f.) Mathiesen (Lamiophlomis rotata (Benth.) Kudô); essential oils; characteristic metabolites; metabolomics; fatty acids; C13-norisoprenoids; biosynthesis; antioxidant activities; pro-oxidant activities

Online: 9 February 2024 (14:02:04 CET)

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Limited research has been conducted on the metabolites present in the essential oils (Es) of Phlomoides rotata (Benth. ex Hook. f.) Mathiesen (PR, syn. Lamiophlomis rotata (Benth.) Kudô), and their potential pharmacological activities, such as antioxidant and anticancer properties, are often overlooked. We conducted a metabolomics-based screening to identify the characteristic metabolites (CMs) present in the Es extracted from PR. Furthermore, we evaluated their in vitro antioxidant activities (AOAs). The Es obtained through hydro-distillation are characterized by a light yellow color and a fresh, elegant fragrance. Cryoprecipitation was used to separate the crystals (Cs) from the Es, resulting in the extraction of crystal-free essential oils (CFs). A total of 107 components belonging to 8 categories were identified and quantified in the Es, Cs, and CFs using GC-MS and GC-FID techniques. Among these components, 76 were reported for the first time in the Es of PR. The major compounds identified include long-chain fatty acids (LCFAs) and their esters, such as palmitic acid (PA), myristic acid (MA), linoleic acid (LA), oleic acid (OA), and methyl palmitate. Additionally, seven C13-norisoprenoids, with trans-β-damascenone being particularly notable for its flavor, were also identified. A sum of nine CMs were identified in the Es, including PA, MA, LA, OA, methyl palmitate, hexahydrofarnesyl acetone, phytol, trans-β-damascenone, and hexanal. The main metabolic pathway in the Es is the biosynthesis of FAs and terpenoids revealed by MetaboAnalyst 6.0 and KEGG analyses. Furthermore, the in vitro AOAs of the Es, Cs, CFs, and four selected CMs (PA, MA, LA, and OA) were evaluated. Generally, PA and MA exhibited pro-oxidant activities (POAs) or weak AOAs. LA and OA demonstrated POAs or weak AOAs at lower concentrations, but at higher concentrations, they displayed medium to strong AOAs. Importantly, the CFs exhibited stronger AOAs compared to the corresponding Es, which may be attributed to their different PA content. Overall, this study provides valuable insights into the potential utilization of Es from PR.

Preprint ARTICLE | doi:10.20944/preprints202402.0559.v1

Patient Experiences of Community Pharmacy Medication Supply and Medicines Reconciliation at Hospital Discharge: A Qualitative Study

Rhona Mundell, Derek Jamieson, Gwen Shaw, Anne Thomson, Paul Forsyth

Subject: Medicine And Pharmacology, Pharmacy Keywords: Hospital Pharmacy; Community Pharmacy; Medication; Qualitative Research

Online: 9 February 2024 (13:20:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0509.v1

The Dry Extracts of Matricaria discoidea DC. Herb with Analgesic and Soporific Activity: Phytochemical, Pharmacological and Molecular Docking Research

Janne Sepp, Oleh Koshovyi, Valdas Jakštas, Vaidotas Žvikas, Iryna Botsula, Igor Kireyev, Hanna I. Severina, Oleksandr Kukhtenko, Kaisa Põhako-Palu, Karin Kogermann, Jyrki Heinämäki, Ain Raal

Subject: Medicine And Pharmacology, Pharmacy Keywords: pineapple weed; herb; extraction; terpenoids; polyphenols; cytotoxicity; analgesic activity; soporific effect

Online: 8 February 2024 (10:18:21 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.0458.v1

Potential Drug Interactions with Cannabinoids in Selected Chronic Diseases: Epilepsy, Autism Spectrum Disorders, Oncology, Multiple Sclerosis and Pain

Maria Graça Campos, Maria China, Mariana Claudio, Miguel Capinha, Rita Torres, Simao Oliveira, Ana Fortuna

Subject: Medicine And Pharmacology, Pharmacy Keywords: Cannabinoids; Tetrahydrocannabinol; CBD; Drug-drug Interactions; F.I.R.E.S.; Cytochrome P450; Epilepsy; Cancer; Autism Spectrum Disorder

Online: 7 February 2024 (17:38:44 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.0430.v1

Rational Prescribing of Pancreatic Enzymes for Patients with Pancreatic Cancer

Mary Acelle Garcia, Syed Imam, Ursula K Braun, Leanne Jackson

Subject: Medicine And Pharmacology, Pharmacy Keywords: pancreatic cancer; exocrine pancreatic insufficiency; pancreatic enzymes; palliative care; medication instruction; compliance

Online: 7 February 2024 (10:13:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1964.v1

Real-Time Cone-Growth Model for Determination of Pharmaceutical Powder Flow Properties

Gyula Farkas, Sándor Ferenc Nagy, Attila Dévay, Aleksandar Széchenyi, Szilárd Pál

Subject: Medicine And Pharmacology, Pharmacy Keywords: flow properties; static angle of repose; flow time; flow curve; conical section

Online: 29 January 2024 (04:02:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1525.v1

Ophthalmic Nanoemulsion Fingolimod Formulation for Topical Application

Rama Kashikar, Samir Senapati, Narendar Dudhipala, Sandip K. Basu, Nawajes Mandal, Soumyajit Majumdar

Subject: Medicine And Pharmacology, Pharmacy Keywords: Fingolimod; nanoemulsion; ocular drug delivery; sterilization; stability; physicochemical characterization

Online: 22 January 2024 (10:08:55 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.1378.v1

Recent Advance Bakuchiol Application as a Potential Alternative to Retinol in Skincare and Cosmetics

Norisca Aliza Putriana, Patihul Husni, Soraya Ratnawulan Mita

Subject: Medicine And Pharmacology, Pharmacy Keywords: Bakuchiol; anti-aging; anti-hyperpigmentation; anti-oxidant; anti-inflammatory; anti-acne; and anti-melanogenesis

Online: 18 January 2024 (07:23:52 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.1363.v1

Treatment Advances for Acne Vulgaris: The Scientific Role of Cannabinoids

Inês Ferreira, Carla M. Lopes, Maria Helena Amaral

Subject: Medicine And Pharmacology, Pharmacy Keywords: acne vulgaris; cannabinoids; cannabidiol; sustainability; lipostatic; anti-inflammatory; antiproliferative; antimicrobial

Online: 18 January 2024 (04:49:39 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.1321.v1

Examining Natural Products with the Potential to Chagas Disease: An In-depth Analysis

Eze Elijah Ajaegbu, Chizurum Philip Ikegbuna

Subject: Medicine And Pharmacology, Pharmacy Keywords: Chagas disease; Trypanosoma cruzi; Natural products; Therapeutic approaches; Anti-parasitic compounds; Plant-derived compounds

Online: 17 January 2024 (12:04:26 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.0803.v1

Flavonoids As CYP3A4 Inhibitors In Vitro

Martin Kondža, Ivica Brizić, Stela Jokić

Subject: Medicine And Pharmacology, Pharmacy Keywords: CYP enzymes; CYP3A4; flavonoid; inhibition; natural products

Online: 10 January 2024 (10:24:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0115.v2

Different antibacterial modes of plant flavonoids to gram-negative from to -positive bacteria

Yu Yan, Xuexue Xia, Aiman Fatima, Li Zhang, Ganjun Yuan, Fengxian Lian, Yu Wang

Subject: Medicine And Pharmacology, Pharmacy Keywords: Flavonoids; Antimicrobial; Lipophilicity; LogP; MIC; Correlation; Bacterium; Escherichia coli; Gram-negative bacteria; mode

Online: 9 January 2024 (02:47:50 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.0554.v1

The Role of Biodegradable Polymers in Nano Carrier-based Drug Delivery System to Enhance Oral Bioavailability & Solubility of BCS Class II, IV Drugs: A Review

Mohan Tripathi, Peeyush Bhardwaj, Shruti Tiwari, Arti Sahu

Subject: Medicine And Pharmacology, Pharmacy Keywords: Biodegradable Polymers; Nanotechnology; Drug Delivery &; Drug Development

Online: 8 January 2024 (06:50:25 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0526.v1

Development of a Versatile Lipid Core for Nanostructure Lipid Carriers (NLC) Using Design of Experiments (DoE) and Raman Mapping

Carlos Alberto Rios, Roberta Ondei, Márcia Cristina Breiktreitz

Subject: Medicine And Pharmacology, Pharmacy Keywords: Nanostructured Lipid Carriers; Raman Mapping; Design of Experiments; Classical Least Squares

Online: 8 January 2024 (06:27:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0441.v1

Overcoming CD Spectroscopy Sample Preparation Challenges in Protein Structural Analysis for Biosimilars

Temitope Agbelusi, Ketan Pancholi, Harini Sampatk Kumar, Amit Kumar

Subject: Medicine And Pharmacology, Pharmacy Keywords: protein; surfactant; SDS; protein-surfactant-interaction; circular-diachorism; Chirality

Online: 5 January 2024 (10:38:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0343.v1

Spray-Drying Microencapsulation of Phenolic Compounds from Bauhinia ungulata L. Var. Obtusifolia Aqueous Extract: A Comparative Study Using Different Wall Materials

Myrth Soares do Nascimento Remigio, Teresa Greco, José-Otávio Carrera Silva-Júnior, Attilio Converti, Roseane Maria Ribeiro-Costa, Alessandra Rossi, Wagner Luiz Ramos Barbosa

Subject: Medicine And Pharmacology, Pharmacy Keywords: Phytotherapy; Medicinal plants; Radical scavenger; Phenolic compounds; Medicinal tea

Online: 4 January 2024 (11:27:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2335.v1

Efficacy of Continuous vs. Intermittent Administration of Cefepime in Adult ICU Patients with Gram‐Negative Bacilli Bacteremia: A Randomized Double‐Blind Clinical Study

Carlos Arturo Álvarez-Moreno, Laura Cristina Nocua-Báez, Guillermo Ortiz, Juan Carlos Torres, Gabriel Montenegro, Williams Cervera, Alonso Gómez, Luis Fernando Zuluaga

Subject: Medicine And Pharmacology, Pharmacy Keywords: bacteraemia; cefepime; sepsis; intravenous infusions

Online: 3 January 2024 (10:05:50 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.0147.v1

Phytochemistry and biological activities of Cissus trifoliata against carcinomas

Luis Fernando Méndez-López

Subject: Medicine And Pharmacology, Pharmacy Keywords: Cissus sp., Phytochemicals, Apoptosis, Proliferation, EMT, Anticancer

Online: 3 January 2024 (05:35:44 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0115.v1

Antibacterial Difference of Plant Flavonoids to Gram-Negative and -Positive Bacteria

Yu Yan, Xuexue Xia, Aiman Fatima, Li Zhang, Ganjun Yuan, Fengxian Lian, Yu Wang

Subject: Medicine And Pharmacology, Pharmacy Keywords: Flavonoids; Antimicrobial; Lipophilicity; LogP; MIC; Correlation; Bacterium; Escherichia coli; Gram-negative bacteria

Online: 3 January 2024 (03:05:43 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2190.v1

Anti-Bacterial and Antioxidant Potential of an Ostrich Oil Emulsion

Juthaporn Ponphaiboon, Sontaya Limmatvapirat, Chutima Limmatvapirat

Subject: Medicine And Pharmacology, Pharmacy Keywords: ostrich oil; emulsion; emulsifier; antibacterial activity; antioxidant activity; stability

Online: 28 December 2023 (11:51:57 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2016.v2

New Carriers for Bioadhesive Gastroretentive Drug Delivery Systems Based on Eudragit® EPO/Eudragit® L100 Interpolyelectrolyte Complexes

Daria S Gordeeva, Aleksandra V Sitenkova (Bukhovets), Rouslan I. Moustafine

Subject: Medicine And Pharmacology, Pharmacy Keywords: interpolyelectrolyte complexes; Eudragit®; ; gastroretentive systems; drug delivery systems; bioadhesive systems; metronidazole; acyclovir

Online: 27 December 2023 (14:28:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2064.v1

A New Frontier in Hypertension Management: Formulating and Characterizing Subcutaneous Implants using Hot Melt Extrusion

Kshitij Chitnis, Nagarjuna Narala, Sagar Narala, Siva Ram Munnangi, Sateesh Kumar Vemula, Michael A Repka

Subject: Medicine And Pharmacology, Pharmacy Keywords: Hypertension, Subcutaneous Implants, Hot Melt Extrusion, Controlled Drug Delivery, Biodegradable, Implantable Drug Delivery

Online: 27 December 2023 (09:45:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2016.v1

New Carriers for Bioadhesive Gastroretentive Drug Delivery Systems Based on Eudragit® EPO/Eudragit® L100 Interpolyelectrolyte Complexes

Daria S Gordeeva, Aleksandra V Sitenkova (Bukhovets), Rouslan I. Moustafine

Subject: Medicine And Pharmacology, Pharmacy Keywords: interpolyelectrolyte complexes; Eudragit®; ; gastroretentive systems; drug delivery systems; bioadhesive systems; metronidazole; acyclovir

Online: 26 December 2023 (14:36:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1212.v1

Spectrophotometric and Chromatographic Assessment of Total Flavonoids Content in Rhodendron Tomentosum Extracts

Halyna Kukhtenko, Nataliia Bevz, Oleksandr Kukhtenko, Izabela Jasicka-Misiak

Subject: Medicine And Pharmacology, Pharmacy Keywords: Rhododendron tomentosum; Ledum palustre; Wild rosemary; Rutin; Hyperoside; Quercetin; Chlorogenic acid; Aluminum chloride; UV-Vis; HPTLC

Online: 18 December 2023 (10:19:01 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.1067.v1

European Association of Hospital Pharmacists (EAHP) Guidance on the Pharmacy Handling of In Vivo Gene Therapy Medicinal Products

Joan VINENT GENESTAR, Sylvain Auvity, Nanna Christiansen, Heidi Ekelund, Liesbeth Huys, Helle Bach Ølgaard McNulty, Marcello Pani, Vera Pires, Bertrand Pourroy, Nicola Stoner

Subject: Medicine And Pharmacology, Pharmacy Keywords: Genetic therapy; Pharmaceutical preparations; Pharmacy service, hospital; Safety; Practice guideline. ;

Online: 14 December 2023 (08:29:10 CET)

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Medicine and Pharmacology