Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Spectrophotometric and Chromatographic Assessment of Total Flavonoids Content in Rhodendron Tomentosum Extracts

Version 1 : Received: 15 December 2023 / Approved: 18 December 2023 / Online: 18 December 2023 (10:19:01 CET)

How to cite: Kukhtenko, H.; Bevz, N.; Kukhtenko, O.; Jasicka-Misiak, I. Spectrophotometric and Chromatographic Assessment of Total Flavonoids Content in Rhodendron Tomentosum Extracts. Preprints 2023, 2023121212. https://doi.org/10.20944/preprints202312.1212.v1 Kukhtenko, H.; Bevz, N.; Kukhtenko, O.; Jasicka-Misiak, I. Spectrophotometric and Chromatographic Assessment of Total Flavonoids Content in Rhodendron Tomentosum Extracts. Preprints 2023, 2023121212. https://doi.org/10.20944/preprints202312.1212.v1

Abstract

In the literature, the chemical composition of Rhododendron tomentosum is mainly represented by the study of isoprenoid compounds of essential oil. In contrast, the study of the content of flavonoids will contribute to the expansion of the pharmacological action and the use of the medicinal plant for medical purposes. The paper deals with the technology of extracts from Rhododendron tomentosum shoots using ethanol of various concentrations and purified water as an extractant. Extracts from Rhododendron tomentosum were obtained by a modified method that combined the effects of ultrasound and temperature to maximize the extraction of biologically active substances from the raw material. By the method of high-performance thin-layer chromatography in the system of solvents ethyl acetate: formic acid: water (15:1:1) the following substances have been separated and identified: rutin, hyperoside, quercetin, and chlorogenic acid in all the extracts obtained. To quantify the total flavonoids content, a method of spectrophotometric determination based on the reaction of complexation with aluminum chloride has been developed. It has been proved that the course of the complexation reaction and, accordingly, the quantification of the flavonoid structure substances by the spectrophotometric method is influenced by some factors, including the environment, pH, the structure of the complexes, etc. Regardless of the chosen method, for the further production of Rhododendron tomentosum extracts with a higher content of flavonoid structure substance, it is advisable to use 60%, 70%, and 80% ethyl alcohol as extractant.

Keywords

Rhododendron tomentosum; Ledum palustre; Wild rosemary; Rutin; Hyperoside; Quercetin; Chlorogenic acid; Aluminum chloride; UV-Vis; HPTLC

Subject

Medicine and Pharmacology, Pharmacy

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.