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Preprint REVIEW | doi:10.20944/preprints202305.1725.v1

Molecular Insights into the Role of Gut Microbiota in Antibiotic Therapy Selection and Resistance Mitigation

Mihaela Andreescu

Subject: Medicine And Pharmacology, Hematology Keywords: Molecular biology; infectious diseases; clinical diagnostic; early detection; prognosis

Online: 25 May 2023 (03:34:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0843.v2

Zanubrutinib: Coming of Age for BTK Inhibitors in the Treatment of Chronic Lymphocytic Leukemia?

Stefano Molica, Constantine Tam, David Allsup, Aaron Polliack

Subject: Medicine And Pharmacology, Hematology Keywords: CLL, Zanubrutinib, BTK inhibitors, efficacy, safety, chronic lymphocytic leukemia

Online: 22 May 2023 (16:15:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1500.v1

Autologous Transplantation May Still Effectively Treat Relapsed Diffuse Large B-cell Lymphoma in Selected Patients

Reka Rahel Bicsko, Lili Antal, Ferenc Magyari, Róbert Szász, Miklós Udvardy, Arpad Illes, Lajos Gergely

Subject: Medicine And Pharmacology, Hematology Keywords: diffuse large B-cell lymphoma; relapsed; refractory; autologous stem cell transplantation

Online: 22 May 2023 (10:29:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1477.v1

Study of the Symptoms’ Severity and Adherence to Therapy of Myelofibrosis Patients Treated With Ruxolitinib

Vera Stoeva, Guenka Petrova, Konstantin Mitov, Konstantin Tihomirov Tachkov

Subject: Medicine And Pharmacology, Hematology Keywords: myelofibrosis; ruxolitinib; severity of symptoms; adherence to therapy

Online: 22 May 2023 (08:52:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1382.v1

Prospective Evaluation of Fetal Hemoglobin Expression in Maternal Erythrocytes: an Analysis of a Cohort of 345 Parturients

Laurence Blain, Christian Watier, Xiaoduan Weng, Andre Masse, Marie-Josée Bédard, Nazila Bettache, Florence Weber, Michele Mahone, Stéphanie Forte, Vincent-Philippe Lavallée, Pierre-Olivier Gaudreau, Michael J. Newmarch, Denis Soulières

Subject: Medicine And Pharmacology, Hematology Keywords: Fetal hemoglobin; pregnancy; glycosylated hemoglobin; HbA1C; β-HCG

Online: 19 May 2023 (05:17:09 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1213.v1

Unravelling the Immune Microenvironment in Classic Hodgkin Lymphoma: Prognostic and Therapeutic Implications

Vasileios Georgoulis, Alexandra Papoudou-Bai, Alexandros Makis, Panagiotis Kanavaros, Eleftheria Hatzimichael

Subject: Medicine And Pharmacology, Hematology Keywords: Hodgkin lymphoma; tumor microenvironment; tumor associated macrophages; CD169+ macrophages; immune evasion; immunosuppression

Online: 17 May 2023 (09:37:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0887.v1

ATO Increases ROS Production and Apoptosis of Cells by Enhancing Calpain-Mediated Degradation of the Cancer Survival Protein TG2

Károly Jambrovics, Szilárd Póliska, Beáta Scholtz, Iván P. Uray, Zoltán Balajthy

Subject: Medicine And Pharmacology, Hematology Keywords: transglutaminase 2; cancer; ATO

Online: 12 May 2023 (05:04:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0700.v1

Autoimmune Limbic Encephalitis in Patients With Hematologic Malignancies After Haploidentical Hematopoietic Stem Cell Transplantation With Post-transplant Cyclophosphamide

Bu Yeon Heo, Myung-Won Lee, Suyoung Choi, Yunju Jung, Thi Thuy Duong Pham, Yunseon Jang, Jung-Hyun Park, Sora Kang, Jeong Suk Koh, Deog-Yeon Jo, Jaeyul Kwon, Ik-Chan Song

Subject: Medicine And Pharmacology, Hematology Keywords: allogeneic hematopoietic stem cell transplantation; autoimmune limbic encephalitis; cyclo-phosphamide; regulatory T cells; CD25; Foxp3; IL-6; fever; acute graft-versus-host disease; cyto-kine-release syndrome

Online: 10 May 2023 (07:46:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202302.0320.v2

Evaluation of a Novel Methodology for Real-Time Calculation of Critical Blood Values Based on Four Measured Values

Rüdiger H. Ettrich, Joshua Caballero, Sultan Ahmed, Traci Hurlston, Jayesh Parmar, Subrata Deb

Subject: Medicine And Pharmacology, Hematology Keywords: non-invasive; point-of-care testing; blood values; Radial arterial blood; blood gas; transcutaneous; algorithm

Online: 4 May 2023 (03:07:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.1236.v1

Gilteritinib: The Story of a Proceeding Success into Hard-to-Treat FLT3 Mutated AML Patients

Matteo Molica, Salvatore Perrone, Marco Rossi

Subject: Medicine And Pharmacology, Hematology Keywords: FLT3 mutations; resistant/relapsed acute myeloid leukemia; tyrosine kinase inhibitors; gilteritinib

Online: 29 April 2023 (10:55:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.1161.v1

Belantamab Mafodotin: From Clinical Trials Data to Real Life Experiences

Sonia Morè, Massimo Offidani, Laura Corvatta, Maria Teresa Petrucci, Francesca Fazio

Subject: Medicine And Pharmacology, Hematology Keywords: Multiple myeloma; belantamab mafodotin; antibody-drug-conjugate.

Online: 28 April 2023 (10:12:31 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0843.v1

Zanubrutinib: Coming of Age for BTK Inhibitors in the Treatment of Chronic Lymphocytic Leukemia?

Stefano Molica, Constantine Tam, David Allsup, Aaron Polliack

Subject: Medicine And Pharmacology, Hematology Keywords: CLL; Zanubrutinib; BTK inhibitors; efficacy; safety; chronic lymphocytic leukemia

Online: 24 April 2023 (09:43:47 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202304.0616.v1

Circulating CD34+/CD38-/CD26+ Leukemia Stem Cells along Chronic Myeloid Leukemia progression: differences between Chronic, Accelerated and Blast Phase

Paola Pacelli, Anna Sicuranza, Elena Bestoso, Alessandra Iurlo, Elisabetta Abruzzese, Adele Santoni, Margherita Malchiodi, Cristina Marzano, Elisabetta Zappone, Donatella Raspadori, Monica Bocchia

Subject: Medicine And Pharmacology, Hematology Keywords: CP-CML; Accelerated Phase (AP); Blast Phase (BP); CD26+LSCs

Online: 20 April 2023 (07:31:53 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0482.v1

The Black Hole: CAR T cell Therapy in AML

Erden Atilla, Karim Benabdellah

Subject: Medicine And Pharmacology, Hematology Keywords: Acute Myeloid Leukemia, cellular therapies, chimeric antigen T cells, T cell receptor T cells, CAR-NK cells, RNAseq

Online: 18 April 2023 (04:22:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202303.0415.v1

Biblical Perspectives as a Guide to Research on Life’s Origin and History

Hugh Ross

Subject: Medicine And Pharmacology, Hematology Keywords: spacetime theorems; naturalism; abiogenesis; panspermia; process structuralism; Cambrian explosion; amino acids; homochirality; hand of God dilemma

Online: 23 March 2023 (13:40:22 CET)

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Preprint REVIEW | doi:10.20944/preprints202303.0371.v1

Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing

Tamer Butto, Kanak Mungikar, Peter Baumann, Jennifer Winter, Beat Lutz, Susanne Gerber

Subject: Medicine And Pharmacology, Hematology Keywords: Nuclei isolation; Next-generation sequencing; Cell-type specific isolation; Epigenetics; Transcriptomics; Single cell sequencing; Single nucleus sequencing

Online: 21 March 2023 (06:40:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0469.v1

Evaluation of the Haematological Patterns from up to 985 Days of Long COVID: A Cross-Sectional Study

Vanessa Costa Alves Galúcio, Daniel Carvalho de Menezes, Patrícia Danielle Lima de Lima, Vera Regina da Cunha Menezes Palácios, Pedro Fernando da Costa Vasconcelos, Juarez Antônio Simões Quaresma, Luiz Fábio Magno Falcão

Subject: Medicine And Pharmacology, Hematology Keywords: Long COVID; Laboratory Markers; Haematological Tests

Online: 27 February 2023 (09:53:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0467.v1

Iron Deficiency and Polycythemia

Rova Malala Fandresena Randrianarisoa, Herveat Ramanandafy, Alexandre Mania, Hélène Monjanel, Sébastien Trouillier

Subject: Medicine And Pharmacology, Hematology Keywords: Diagnostic; Iron deficiency; Polycythemia; Polycythemia vera; Secondary polycythemia

Online: 27 February 2023 (09:36:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0320.v1

A Novel Noninvasive Technology for Real-Time Assessment of Critical Blood Values: A Pilot Clinical Study

Rüdiger H. Ettrich, Joshua Caballero, Sultan Ahmed, Traci Hurlston, Jayesh Parmar, Subrata Deb

Subject: Medicine And Pharmacology, Hematology Keywords: non-invasive; point-of-care testing; blood values; Radial arterial blood; blood gas; transcutaneous; algorithm

Online: 20 February 2023 (06:07:08 CET)

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There are several point-of-care testing (POCT) devices for measuring critical laboratory values, but real-time utility can be cumbersome considering need for blood draws, multiple cartridges, and potential for delays in obtaining results. Digital Blood Corporation (DBC) developed an algorithm for non-invasive, real-time measurement of multiple values. The objective of this study was to compare the values obtained using a POCT device with the DBC’s non-invasive methodology and to evaluate the robustness of the algorithm. After Institutional Review Board approval, a pilot and feasibility study was conducted in healthy ambulatory individuals aged 18-64 years. Radial arterial blood was collected for the POCT comparator analysis using the Abbott i-STAT® device. The four parameters directly measured for DBC’s algorithm included temperature, hemoglobin, and partial pressures of oxygen and carbon dioxide (pO2 and pCO2, respectively). Using these values, the algorithm calculated sodium, potassium, chloride, ionized calcium (iCa), total carbon dioxide (TCO2), pH, bicarbonate, and oxygen saturation (SO2). Bland-Altman difference plot were calculated for analyzing the agreement between the Abbott i-STAT® device and DBC’s non-invasive methodology. For a second set of data, pO2 and pCO2 values collected using the Abbott i-STAT® device were used as input for DBC’s algorithm to test robustness of the algorithm. Data from 37 participants (mean age: 42.4 + 13 years; range: 18-64 years) were included in the primary analysis. Mean difference was less than 5% for sodium, chloride, pH, SO2, and bicarbonate; 6-10% for hemoglobin, TCO2 and pCO2; and greater than 10% for iCA, potassium, and pO2. The greatest variation between POCT and DBC’s approach was observed for pO2. Algorithm values that depend upon pO2 precision obtained from the TINA TCM4 radiometer are expected to show greatest deviation. Replacing transcutaneous pO2 and pCO2 values from the TINA TCM4 instrument with Abbott i-STAT values demonstrates the robustness of DBC’s algorithm and its ability to predict blood values comparable to the POC device in healthy patients. This pilot study serves as a proof of concept to trigger future study and further development of the DBC’s non-invasive device in critical care settings and test the usability of the device via quantitative and qualitative measures with different healthcare providers (e.g., pharmacists, nurses, paramedics). The broader clinical significance especially among critically ill adults and children remains to be determined.

Preprint ARTICLE | doi:10.20944/preprints202302.0311.v1

Morphology and Hemodynamics of Cerebral Arteries and Aneurysms in a Pair of Monozygotic Twins

Hang Yi, Zifeng Yang, Luke C Bramlage, Ludwig R Bryan

Subject: Medicine And Pharmacology, Hematology Keywords: Monozygotic twins (MTs); morphology; hemodynamics; environmental and genetic factors; neurovascular diseases; cerebral aneurysms (CAs)

Online: 17 February 2023 (13:01:18 CET)

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The contribution of genetic and environmental factors to the pathophysiology of cerebral aneurysms in monozygotic twins is under-reported and presents ambiguous arguments. The morphology and hemodynamics of neurovascular arteries in a pair of monozygotic twins (MTs) were investigated to reveal the underlying mechanisms. Four arterial models were reconstructed for the twin A-right brain and left brain, twin B-left brain, and B-left brain without anterior cerebral arteries based on preclinical scanned information. Subsequently, the dimensions, configurations and outlined curves of the three-perspective geometries were compared between the MTs. Adopting an in-vitro validated numerical cerebral aneurysm model, hemodynamic patterns were investigated and compared in the MT models, respectively. Morphological comparisons of the MTs show the size and shape of cerebral arteries exist significant differences, despite of the expected genetic similarities. These differences can be attributed to variations during embryological development and external environmental influences. Qualitatively and generally, numerical results indicate the MTs have some hemodynamic similarities (e.g., time-averaged pressure (TAP) distributions (~13400 Pa), and oscillatory shear index (0~0.49), but present significant differences in specific local arteries due to morphological variances. Specifically, the difference in the volumetric blood flow rate in corresponding arteries between the MTs is from 16% (smallest) in anterior choroidal artery (AChA) to 221% (largest) in the ophthalmic artery (OphA), varying with specific compared arteries. Also, the registered hemodynamic indicators, such as the maximum time-averaged wall shear stress (TWSS) (53.6 Pa vs. 37.8 Pa), and different local OSI distributions were observed between the MTs. The findings revealed that morphological variations in MTs could be generated by embryological and environmental factors, thus assuming they share the identical morphology in cardiovascular and neurovascular systems may lead to significant misevaluations in hemodynamics quantifications and further lesions.

Preprint ARTICLE | doi:10.20944/preprints202212.0368.v1

A Synergistic pH-Responsive serum albumin-based drug delivery system loaded with doxorubicin and pentacyclic triterpene betulinic acid for potential treatment of NSCLC

Zally Torres-Martinez, Daraishka Pérez, Grace Torres, Sthephanie Estrada, Clarissa Correa, Natasha Mederos, Kimberly Velazquez, Betzaida Castillo, Kai Griebenow, Yamixa Delgado

Subject: Medicine And Pharmacology, Hematology Keywords: Betulinic acid; bovine serum albumin; doxorubicin; drug delivery system; lung cancer; synergistic effect.

Online: 20 December 2022 (10:40:12 CET)

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Nanosized drug delivery systems (DDS) have been studied as a novel strategy against cancer due to their potential to simultaneously decrease drug inactivation and systemic toxicity and increase passive and/or active drug accumulation within the tumor(s). Triterpenes are plant-derived compounds with interesting therapeutic properties. Betulinic acid (BeA) is a pentacyclic triterpene which has great cytotoxic activity against different cancer types. Herein, we developed a nanosized protein-based DDS of bovine serum albumin (BSA) as the drug carrier combining two compounds: doxorubicin (Dox) and the triterpene BeA using an oil-water-like micro-emulsion method. Spectrophotometric assays were performed to determine protein and drug concentrations in the DDS. The biophysical properties of these DDS were characterized using dynamic light scattering (DLS) and circular dichroism (CD) spectroscopy confirming nanoparticle (NP) formation and drug loading into the protein structure, respectively. The encapsulation efficiency was 77% for Dox and 18% for BeA. More than 50% of both drugs were released within 24 h, at pH 6.8, while less drug was released at pH 7.4 in this time period. Co-incubation viability assays of Dox and BeA alone for 24 h demonstrated synergistic cytotoxic activity in the low μM range against the non-small cell lung carcinoma (NSCLC) A549 cells. Viability assays of the BSA(Dox+BeA) DDS demonstrated a higher synergistic cytotoxic activity than the two drugs with no carrier. Moreover, confocal microscopy analysis confirmed cellular internalization of the DDS and nucleus accumulation of the Dox. We determined the mechanism of action of the BSA(Dox+BeA) DDS, confirming S-phase cell cycle arrest, DNA damage, caspase cascade activation, and downregulation of the epidermal growth factor receptor (EGFR) expression. This DDS has the potential to synergistically maximize the therapeutic effect of Dox and diminish chemoresistance induced by EGFR expression using a natural triterpene against NSCLC.

Preprint ARTICLE | doi:10.20944/preprints202212.0152.v1

Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Massimo Sangiorgi, Mariangela Valentina Puci, Allegra Rosa Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia Di Pinto, Franco Michelino Fiore, Armado Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massara, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Pietro Modugno, Maurizio Maiorano, Umberto Marcello Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni Maria Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaó, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio Stefano Tolva, Ilenia D'Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D'Arrigo, Giusi Basile, Dalmazio Frigerio, Gian Franco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio Massimo Oddi, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: chronic limb-threatening ischemia; outcome; sex; age; limb salvage

Online: 8 December 2022 (09:39:30 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0087.v1

Interrelation Midst Low T3 Levels, Type 3 Deiodinase, Oxidative Stress And Mortality In Sepsis and Septic Shock: Defining Patient Outcome

Josi Vidart, Luiza Axelrud, Andre Cardozo Braun, Rafael A. Marschner, Simone Magagnin Wajner

Subject: Medicine And Pharmacology, Hematology Keywords: low T3 syndrome, oxidative stress, sepsis, septic shock, thyroid hormone, type 3 deiodinase

Online: 6 December 2022 (02:18:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202210.0002.v1

Therapeutic Approach of Anticoagulants and Acute Blood Purification for Patients with Sepsis-Induced Disseminated Intravascular Coagulation: Post-marketing Surveillance Data of Antithrombin Supplementation

Yutaka Eguchi, Hiroyuki Nagafuchi, Toshiaki Ikeda

Subject: Medicine And Pharmacology, Hematology Keywords: antithrombin; recombinant thrombomodulin; SOFA score; JAAM DIC score; PMX-DHP

Online: 1 October 2022 (07:09:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0299.v1

Blood Groups (ABO/Rh) And Sociodemographic and Clinical Profile among Patients with Leprosy in Angola

Euclides Nenga Manuel Sacomboio, Tomásia Oliveira Muhongo, Adelino Tchilanda Tchivango, Edson Kuatelela Cassinela, Silvana da Rocha Silveira, Mauricio Da Costa, Carlos Alberto Pinto de Sousa, Cruz Sebastião Sebastião, Eduardo Ekundi Valentim

Subject: Medicine And Pharmacology, Hematology Keywords: leprosy; ABO/Rh blood group; Clinical; Angola

Online: 20 September 2022 (09:28:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0114.v1

Assessment of Knowledge, Attitude, and Practice toward Blood Transfusion among Healthcare Providers in Qatar

Danial Hassan, Abdulqadir J Nashwan, Mohamed Yassin

Subject: Medicine And Pharmacology, Hematology Keywords: Blood Transfusion; Knowledge; Attitude; Practice; Healthcare Providers

Online: 8 September 2022 (03:02:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0193.v1

Association between Liver Function Indicators, Immunoglobulins, and Toll-like Receptors and Neuropilin-1 Expression in Patients with COVID-19

Salma A. El Kazafy, Amr E. Ahmed, Yasser M. Fouad, Azza F. Said, Tarek M. Ali, Ahmad EI Askary, Hebatallah H. Assal, Osama M. Ahmed

Subject: Medicine And Pharmacology, Hematology Keywords: liver; Neuropilin-1; Toll-like receptors; COVID-19

Online: 10 August 2022 (05:05:04 CEST)

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Purpose: The study aimed to investigate if there were any links between liver function biomarkers and immunoglobulins levels in serum, and Toll-like receptors (TLRs) and neuropilin-1 (NRP1) in COVID-19 patients. The study also aimed to assess the accuracy—sensitivity, specificity, and area under the curve (AUC) by the receiver operator curve (ROC) analysis for immunoglobulins levels and TLRs expressions. Patients and Methods: This study included 150 patients (100 patients with confirmed COVID-19 and 50 healthy volunteers as a control group). Patients with COVID-19 were subdivided into two groups according to the severity of symptoms (moderate and severe, with 50 patients each). Serum C-reactive protein (CRP), alanine aminotransaminase (ALT), aspartate aminotransaminase (AST), albumin, lactate dehydrogenase (LDH), immunoglobulin (Ig) G, and IgM levels were estimated. TLRs (TLR2 and TLR4) and NRP1 gene expression in blood samples were investigated using quantitative real-time polymerase chain reaction (qRT-PCR). ROC analysis was also applied to determine the accuracy of various detected parameters in predicting the possibility of COVID-19 infection. Results: In COVID-19 patients, serum parameters related to liver function, except serum albumin, CRP, IgG, IgM, and TLR2, TLR4, and NRP1 mRNA expression levels, significantly elevated compared to controls. Severe COVID-19 patients exhibited significantly higher liver enzymes (ALT, AST and LDH), CRP, and TLR2 mRNA expression levels and lower albumin levels than the moderate group. In the moderate and severe groups, ALT, CRP, TLR2, and TLR4 had a significant positive correlation with IgM levels. ALT, AST, LDH, CRP, TLR2, and TLR4 showed a significant positive correlation with IgG levels in both groups. In both the moderate and severe groups, NRP1 expression was found to be significantly correlated with CRP, IgG, IgM, TLR2, and TLR4. In contrast, serum albumin levels exhibited a significant negative correlation with IgG and IgM levels only in the severe group, but they showed a significant negative correlation with TLR2, TLR4, and NRP1 expression in both moderate and severe groups. Serum ALT and AST activities were positively correlated with NRP1 expression in the moderate group but not in the severe group and as well as TLR2 and TLR4 expression in both the moderate and severe groups. ROC analysis indicated that AUC was higher than 0.800 for serum IgM level and TLR4 gene expression in moderate COVID-19 group. Conclusions: The increased liver function biomarkers in serum and NRP1 expression are closely correlated with sustained activations in humoral and innate immune responses during COVID-19 infection. As a result, TLR2, TLR4, and NRP1 could be targets for limiting COVID-19 infection and impairment effects on liver function. Moreover, detection of IgM level in serum and TLR4 expression in blood have a good accuracy in predicting the possibility of infection with COVID-19 in moderate cases.

Preprint ARTICLE | doi:10.20944/preprints202204.0058.v2

Assessment of PVS Filtering Methods Using a Three-Dimensional Computational Model

Jose Bernal, Maria Valdés-Hernández, Javier Escudero, Roberto Duarte, Lucia Ballerini, Mark Bastin, Ian Deary, Michael Thrippleton, Rhian Touyz, Joanna Wardlaw

Subject: Medicine And Pharmacology, Hematology Keywords: Digital reference object; Perivascular spaces; Spatio-temporal imaging artefacts; Perivascular space enhancement; Cerebral small vessel disease

Online: 3 August 2022 (11:12:43 CEST)

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Preprint REVIEW | doi:10.20944/preprints202206.0424.v1

COVID-19 Associated Thrombotic Thrombocytopenic Purpura: A Case Report and Systematic Review

Haseeb Chaudhary, Usama Nasir, Khezar Syed, Maria Labra, Christopher Reggio, Ansar Aziz, Parin Shah, Roopkia Reddy, Navdeep Sangha

Subject: Medicine And Pharmacology, Hematology Keywords: SARS-COV-2; COVID-19; TTP; refractory; thrombotic microangiopathies

Online: 30 June 2022 (09:06:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0230.v1

How the First Year of the COVID-19 Pandemic Impacted Patients’ Care in the Vascular Surgery Divisions of the Southern Regions of the Italian Peninsula

Eugenio Martelli, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Sangiorgi, Matilde Zamboni, Allegra Rosa Martelli, Giancarlo Accarino, Giuseppe Bianco, Francesco Bonanno, Umberto Marcello Bracale, Enrico Cappello, Giovanni Cioffi, Giovanni Colacchio, Adolfo Crinisio, Salvatore De Vivo, Carlo Patrizio Dionisi, Loris Flora, Giovanni Impedovo, Francesco Intrieri, Luca Iorio, Gabriele Maritati, Piero Modugno, Mario Monaco, Giuseppe Natalicchio, Vincenzo Palazzo, Fernando Petrosino, Francesco Pompeo, Raffaele Pulli, Davide Razzano, Maurizio Ruggero Ruggieri, Carlo Ruotolo, Paolo Sangiuolo, Gennaro Vigliotti, Pietro Volpe, Antonella Biello, Pietro Boggia, Michelangelo Boschetti, Enrico Maria Centritto, Flavia Condò, Lucia Cucciolillo, Amodio Salvatore D'Amodio, Mario De Laurentis, Claudio Desantis, Daniela Di Lella, Giovanni Di Nardo, Angelo Disabato, Ilaria Ficarelli, Angelo Gasparre, Antonio Nicola Giordano, Alessandro Luongo, Mafalda Massara, Vincenzo Molinari, Andrea Padricelli, Marco Panagrosso, Anna Petrone, Serena Pisanello, Roberto Prunella, Michele Tedesco, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: COVID-19; carotid stenosis; abdominal aortic aneurysm; chronic limb-threatening ischemia; amputation; deep venous thrombosis

Online: 16 June 2022 (04:40:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0058.v1

Assessment of PVS Enhancement Methods Using a Three-Dimensional Computational Model

Jose Bernal, Maria Valdés-Hernández, Javier Escudero, Roberto Duarte, Lucia Ballerini, Mark Bastin, Ian Deary, Michael Thrippleton, Rhian Touyz, Joanna Wardlaw

Subject: Medicine And Pharmacology, Hematology Keywords: Digital reference object; Perivascular spaces; Spatio-temporal imaging artefacts; Perivascular space enhancement; Cerebral small vessel disease

Online: 7 April 2022 (11:27:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202201.0208.v1

Advance Directives in Oncology and Haematology: A Long Way to Go. A Narrative Review

Kevin Serey, Amélie Cambriel, Adrien Pollina-Bachellerie, Jean-Pierre Lotz, François Philippart

Subject: Medicine And Pharmacology, Hematology Keywords: End of Life; Advance Directives; Advance Care Planning; Intensive Care, Medical Oncology; malignant hemopathy

Online: 14 January 2022 (11:34:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0207.v2

Hematological and Biochemical Reference Ranges for Population With Sickle Cell Disease at Steady-State in Tanzania

Anna Daniel Fome, Raphael Zozimus Sangeda, Emmanuel Balandya, Josephine Mgaya, Deogratius Soka, Furahini Tluway, Upendo Masamu, Siana Nkya, Julie Makani, Bruno P. Mmbando

Subject: Medicine And Pharmacology, Hematology Keywords: Hematological parameters; biochemical parameters; reference ranges; sickle cell disease; Tanzania; steady-state.

Online: 11 January 2022 (12:54:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0207.v1

Hematological and Biochemical Reference Ranges for Population With Sickle Cell Disease at Steady-State in Tanzania

Anna Daniel Fome, Raphael Z. Sangeda, Josephine Mgaya, Deogratius Soka, Furahini Tluway, Upendo Masamu, Emmanuel Balandya, Siana Nkya, Julie Makani, Bruno P. Mmbando

Subject: Medicine And Pharmacology, Hematology Keywords: Hematological parameters; biochemical parameters; reference ranges; sickle cell disease; Tanzania; steady-state.

Online: 13 December 2021 (13:33:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0578.v1

Diphenyleneiodonium Triggers Cell Death of Acute Myeloid Leukaemia Cells by Blocking the Mitochondrial Respiratory Chain and Synergizes with Cytarabine

Hassan Dakik, Maya El Dor, Jérôme Bourgeais, Farah Kouzi, Olivier Herault, Fabrice Gouilleux, Kazem Zibara, Frédéric Mazurier

Subject: Medicine And Pharmacology, Hematology Keywords: DPI; Mitochondria; Leukaemia; Oxidative stress; OxPhos; Ara-C

Online: 30 November 2021 (21:37:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0438.v1

Superb Microvascular Imaging as a Novel Tool for the Assessment of Blood Flow Velocity in Patients with Systemic Sclerosis: A Single-Center Feasibility Study

Jan-Gerd Rademacher, Rosa Marie Buschfort, Thomas Asendorf, Viktor Korendovych, Björn Tampe, Peter Korsten

Subject: Medicine And Pharmacology, Hematology Keywords: Systemic Sclerosis; Raynaud’s phenomenon; ultrasound; microvascular imaging; blood flow

Online: 23 November 2021 (16:15:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0320.v1

Elastic Laminar Reorganization Occurs with Outward Diameter Expansion during Collateral Artery Growth and Requires Lysyl Oxidase for Stabilization

Ryan M McEnaney, Dylan D McCreary, Nolan Skirtich, Elizabeth Andraska, Ulka Sachdev, Edith Tzeng

Subject: Medicine And Pharmacology, Hematology Keywords: Arteriogenesis; Arterial structure; extracellular matrix; peripheral arterial disease; collateral circulation

Online: 18 November 2021 (10:59:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202110.0404.v1

The 4-Year Experience with Implementation and Routine Use of Pathogen Inactivation in a Brazilian Hospital

Roberta Maria Fachini, Rita Fontão-Wendel, Ruth Achkar, Patrícia Scuracchio, Mayra Brito, Marcelo Amaral, Silvano Wendel

Subject: Medicine And Pharmacology, Hematology Keywords: pathogen reduction; blood safety; platelet transfusion; INTERCEPT; plasma

Online: 27 October 2021 (12:27:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202108.0448.v2

Protein Biomarkers in Blood Reflect the Interrelationships between Stroke Outcome, Inflammation, Coagulation, Adhesion, Senescence and Cancer

Georg Fuellen, Jan Böhmert, Larissa Henze, Daniel Palmer, Uwe Walter, Soyoung Lee, Clemens Schmitt, Henrik Rudolf, Axel Kowald

Subject: Medicine And Pharmacology, Hematology Keywords: stroke; cellular senescence; coagulation; adhesion

Online: 14 October 2021 (15:21:58 CEST)

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Preprint REVIEW | doi:10.20944/preprints202109.0127.v1

Is 6-Shogaol a Beneficial Phytochemical for Patients with Lower-Risk Myelodysplastic Syndrome? A Narrative Review

Soo Liang Ooi, Ron Campbell, Sok Cheon Pak, Terry Golombick, Arumugam Manoharan, Raj Ramakrishna, Vladimir Badmaev, Janet Schloss

Subject: Medicine And Pharmacology, Hematology Keywords: Zingiber officinale; 6-Shogaol; 6-gingerol; hematopoiesis; hepcidin; anaemia

Online: 7 September 2021 (12:08:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202108.0448.v1

Protein Biomarkers in Blood Reflect the Interrelationships between Stroke Outcome, Inflammation, Coagulation, Adhesion, Senescence and Cancer

Georg Fuellen, Jan Böhmert, Larissa Henze, Daniel Palmer, Uwe Walter, Axel Kowald

Subject: Medicine And Pharmacology, Hematology Keywords: stroke; cellular senescence; coagulation; adhesion

Online: 23 August 2021 (14:01:30 CEST)

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Preprint REVIEW | doi:10.20944/preprints202107.0203.v1

Polymorphisms of Genes Related to Phase-II Metabolism and the Resistance of Clopidogrel

Abdullah Alkattan, Ahmed alkhalifah, Eman Alsalameen, Fatimah Alghanim, Nashwa Radwan

Subject: Medicine And Pharmacology, Hematology Keywords: Polymorphism; genes; phase-II metabolism; glutathione; clopidogrel resistance

Online: 8 July 2021 (13:30:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0322.v1

Platelet Function and Microvesicles Generation in Patients with Hemophilia A

Antonia Melero-Amor, Paola Romecín, David Iyú, David García-Bernal, Esther García Navarro, José M. Moraleda, Joaquin Garcia-Estañ, Faustino García-Candel, Noemí M. Atucha

Subject: Medicine And Pharmacology, Hematology Keywords: Hemophilia A; Prophylaxis; Factor VIII; Platelets; Microvesicles; Calcium

Online: 14 September 2020 (16:12:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202006.0275.v1

Analysis of the Primary Presenting Symptoms and Hematological Findings of COVID-19 Patients in Bangladesh

Abu Taiub Mohammed Mohiuddin Chowdhury, Md Rezaul Karim, H.M. Hamidullah Mehedi, Mohammad Shahbaz, Md Wazed Chowdhury, Guo Dan, Shuixiang He

Subject: Medicine And Pharmacology, Hematology Keywords: SARS-CoV-2; COVID-19; real-time RT-PCR; COVID-19 symptoms; COVID-19 hematological findings; Bangladesh

Online: 21 June 2020 (14:47:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202006.0062.v1

A Narrative Review on Evaluation of Hypercoagulability State in Severe COVID-19 Patients with Background Risk Factors

Minoosh Moghimi, Kasra Khodadadi, Yousef Mortazavi

Subject: Medicine And Pharmacology, Hematology Keywords: Covid19; DIC; coagulopathy; Sars Covid; blood coagulation disorder

Online: 7 June 2020 (07:41:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202006.0001.v1

Increased Zinc and Albumin but Lowered Copper in Children with Transfusion-dependent Thalassemia

Zainab Alhillawi, Hussein Al-Hakeim, Shatha Moustafa, Michael Maes

Subject: Medicine And Pharmacology, Hematology Keywords: Copper; transfusion-dependent thalassemia; zinc; oxidative stress; antioxidants; biomarkers

Online: 2 June 2020 (09:21:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202002.0454.v1

Prevalence of Blood Groups in Eastern UP Population

Pradeep Kumar, Vandana Rai

Subject: Medicine And Pharmacology, Hematology Keywords: ABO blood groups; antigen; allele frequency; phenotype

Online: 29 February 2020 (08:26:18 CET)

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Working Paper ARTICLE

Diagnosis of Inherited Platelet disorders on a Blood Smear

Carlo Zaninetti, Andreas Greinacher

Subject: Medicine And Pharmacology, Hematology Keywords: inherited platelet disorders; hereditary thrombocytopenias; blood smear; immunofluorescence; bleeding tendency

Online: 20 January 2020 (09:50:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints201910.0283.v1

Concentrations of C-Reative Protein and Leukocytes as Predictors of Adverse Health Factors in the Period of 30 Days Post-Hospital Discharge

Nayara Araújo dos Reis, Rosalina Aparecida Partezani Rodrigues, Darlene Mara dos Santos Tavares, Jack Roberto Silva Fhon, Jair Sindra Virtuoso Júnior

Subject: Medicine And Pharmacology, Hematology Keywords: chronic inflammation; biomarker panels; leukocyte count; C-reactive protein; related syndromes and pathologies; risk assessment; screening programmes; ageing; elderly's health

Online: 25 October 2019 (04:16:43 CEST)

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Preprint REVIEW | doi:10.20944/preprints201908.0199.v1

Concentrations of PCDD/Fs in Human Blood: A Review of Data from the Current Decade

Montse Marquès, Jose L Domingo

Subject: Medicine And Pharmacology, Hematology Keywords: polychlorinated dibenzo-p-dioxins (PCDDs); polychlorinated dibenzo-p-furans (PCDFs); human exposure; blood samples; dietary intake; risks

Online: 20 August 2019 (04:20:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints201809.0435.v1

Targeting Oncogenic Signaling in Mutant FLT3 Acute Myeloid Leukemia: the Path to Least Resistance

Dilana Staudt, Heather Murray, Frank Alvaro, Anoop Enjeti, Nicole Verrills, Matthew D. Dun

Subject: Medicine And Pharmacology, Hematology Keywords: Acute Myeloid Leukemia; FLT3; Tyrosine kinase inhibitors; resistance

Online: 21 September 2018 (10:28:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201807.0422.v1

Saliva and Serum Ghrelin and Obestatin in Iron Deficiency Anemia Patients

Kader Ugur, Suleyman Aydin, Emir Donder, İbrahim Sahin, Meltem Yardim, Mehmet Kalayci, Nevzat Gozel, Ramazan Ulu, Muhammed Sait Dag, Murat Sarikaya

Subject: Medicine And Pharmacology, Hematology Keywords: iron deficiency anemia; obestatin; ghrelin

Online: 23 July 2018 (12:34:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201709.0100.v1

Cut-off Values of Hematologic Parameters to Predict the Number of Alpha Genes Deleted in Subjects with Deletional Alpha Thalassemia

Diego Velasco-Rodríguez, Carlos Blas, Juan-Manuel Alonso-Domínguez, Gala Vega, Carlos Soto, Aránzazu García-Raso, Pilar Llamas-Sillero

Subject: Medicine And Pharmacology, Hematology Keywords: alpha; thalassemia; deletional; cut-off; number of genes; microcytic anemia; differential diagnosis

Online: 21 September 2017 (04:47:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201705.0037.v1

Selection of an Animal Model for the Production of Leukocyte-Fibrin Rich Platelet Membranes (L-PRF); Standardized Protocol Proposed for Clinical Use and the Use of L-PRF Wound Box^®

Alessandro Crisci, Domenico Lombardi, Edda Serra, Guido Lombardi, Federica Cardillo, Michela Crisci

Subject: Medicine And Pharmacology, Hematology Keywords: autologous; buffy coat; growth factor level; platelet-rich fibrin; thrombocyte concentrate

Online: 4 May 2017 (08:37:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201610.0005.v1

Socio Environmental and Haematological Profile of Landfill Residents (São Jorge Landfill – Sao Paulo – Brazil)

Vivianni Wanderley, Fernando Luiz Affonso Fonseca, André Vala Quiaios, José Nuno Domingues, Susana Paixão, João Figueiredo, Ana Ferreira, Cleonice de Almeida Pinto, Odair Ramos da Silva, Rogério Alvarenga, Amaury Machi Junior, Eriane Justo Luiz Savóia, Rodrigo Daminello Raimundo

Subject: Medicine And Pharmacology, Hematology Keywords: Landfill, Waste, Socio-environmental impact, Hematologic diseases

Online: 4 October 2016 (09:03:17 CEST)

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Medicine and Pharmacology