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Preprint ARTICLE | doi:10.20944/preprints202305.1941.v1

Coping as a Mediator between Attachment and Depression either in Pregnancy or in the Early Postpartum Period: A Structural Equation Modelling Approach

Mario Altamura, Ivana Leccisotti, Laura De Masi, Fiammetta Gallone, Livia Ficarella, Melania Severo, Simona Biancofiore, Antonio Ventriglio, Annamaria Petito, Giuseppe Maruotti, Luigi Nappi, Antonello Bellomo

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: Perinatal Depression; Attachment Style; Coping Strategies; Gender Medicine; Women Health

Online: 26 May 2023 (14:42:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1937.v1

Prospective Clinical Trials to Advance the Study of Immunotherapy Toxicities

Christopher Cluxton, Jarushka Naidoo

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Cancer Immunotherapy; Tumour Immunology; Clinical trials; Adverse effects

Online: 26 May 2023 (14:16:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1927.v1

Impacts of COVID-19 Pandemic on Seizure Control in Children with Epilepsy

Ja Un Moon, Da Hye Yoon, Min Ho Jung

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: COVID19; Children; Epilepsy; Adolescents; Pediatric; Pandemic

Online: 26 May 2023 (11:00:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1917.v1

Serum Biomarker Concentrations on Admission in Acute Traumatic Brain Injury: Associations with, TBI Severity, Toxoplasma gondii Infection and Outcome in a Referral Hospital Setting, Cameroon

Franklin Chu Buh, Germaine Sotoing Taiwe, Firas H. Kobeissy, Kevin W. Wang, Andrew I.R. Maas, Basil Kum Meh, Motah Mathieu, Eric Youm, Peter J.A. Hutchinson, Irene Ule Ngole Sumbele

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Serum biomarkers; Traumatic brain injury; CT-scan findings; outcome; Toxoplasma gondii

Online: 26 May 2023 (10:28:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1916.v1

Improved Arterial Oxygenation by High Flow Nasal Cannula Oxygen Therapy in Older Patients with Severe Respiratory Failure in a Non-intensive Hospital Ward

Filippo Luca Fimognari, Valentina Bambara, Giuseppe Armentaro, Paola Scarpino, Chiara Settino, Marco Filice, Massimo Rizzo, Angela Sciacqua

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: High flow nasal cannula oxygen therapy; acute respiratory failure; geriatrics; acute non-intensive hospital setting

Online: 26 May 2023 (10:22:17 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1908.v1

Clinical and Epidemiological Features, Mechanism of Action, and Diagnosis of Global Pandemic Infectious Disease, COVID-19, and its Comparison with SARS, MERS, and H1N1: A Brief Overview

Saeed Reza Hormozi Jangi

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; Mechanism of Action; Clinical and Epidemiological Features; Global Pandemic Infectious Disease

Online: 26 May 2023 (09:59:10 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202305.1906.v1

Congenital Cytomegalovirus (cCMV) From Non-Primary Maternal Infection: Still a Problem?

Victoria Malherbe, Stefanie Celen, Katherine Carkeek³, Evelina Carapancea, Cinzia Auriti, Fiammetta Piersigilli

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: Congenital cytomegalovirus; hypothermia; status epilepticus; universal screening

Online: 26 May 2023 (09:51:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1905.v1

A Novel Anti-CD44 Variant 10 Monoclonal Antibody is Useful for Immunohistochemical Analysis against Oral Squamous cell carcinomas

Kenichiro Ishikawa, Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: CD44; CD44v10; monoclonal antibody; oral squamous cell carcinoma; immunohistochemistry

Online: 26 May 2023 (09:49:30 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202305.1900.v1

Perforating Gout: Expanding the Differential for Transepidermal Elimination

Michal Bohdanowicz, Scott H Bradshaw

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Gout; Tophus; Perforating Dermatosis; Transepidermal Elimination; Pseudoepitheliomatous Hyperplasia; Granulomatous Dermatitis

Online: 26 May 2023 (09:36:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1899.v1

The Effect of Caffeine on the Heart Rate Variability in Newborns: A Pilot Study

Helena Lenasi, Eva Rihar, Jerneja Filipič, Matjaž Klemenc, Petja Fister

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: newborn; heart rate variability; caffeine; apnoea; apnea; autonomic nervous system

Online: 26 May 2023 (09:36:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1889.v1

Clinical Applications of Endobronchial Ultrasound: Challenges and Opportunities

Davide Biondini, Mariaenrica Tinè, Umberto Semenzato, Matteo Daverio, Francesca Scalvenzi, Erica Bazzan, Graziella Turato, Marco Damin, Paolo Spagnolo

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: Endobronchial ultrasound; Transbronchial needle aspiration; bronchoscopy

Online: 26 May 2023 (08:16:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1884.v1

Performing Advanced Trauma Life Support (ATLS) across Borders -Mid-Term Follow Up of the Aeromedical Evacuation after Civilian Bus Accident at Madeira

Sebastian Imach, Andreas Deschler, Stefan Sammito, Miguel Reis, Sylta Michaelis, Ben Marche, Thomas Paffrath, Bertil Bouillon, Thorsten Tjardes

Subject: Medicine And Pharmacology, Emergency Medicine Keywords: 1. traumatology; 2. Trauma surgery; 3. disaster medicine; 4. patient transfer; 5. trauma rehabilitation; 6. Advanced Trauma Life Support Care; 7. air ambulances

Online: 26 May 2023 (07:55:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1877.v1

From Dual Roles to Translational Challenges: Unpacking the Complexities of Tumor-Associated Macrophages in Cancer Progression and Therapy

Shengwen Shao, HuiLai Miao, Wenxue Ma

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Tumor-associated macrophages (TAM); Tumor microenvironment (TME); Cytokine; Immunosuppression; Pro-tumor functions; Anti-tumor functions; Clinical application.

Online: 26 May 2023 (07:31:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1876.v1

Quantification of Analgesic and Anti-inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane

Vladimír Vrkoslav, Ingrida Smeringaiova, Natalia Smorodinova, Alzbeta Svobodova, Stepan Strnad, Catherine Joan Jackson, Jan Burkert, Katerina Jirsova

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: amniotic membrane allografts; cryopreserved amniotic membrane; lyophilization; freeze-dried amniotic membrane; N-acylethanolamines; palmitoylethanolamide; tissue banking; mass spec-trometry

Online: 26 May 2023 (07:29:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1875.v1

Alterations in the Epigenetic Machinery Associated With Prostate Cancer Health Disparities

Jenna Craddock, Jue Jiang, SEAN PATRICK, Shingai Mutambirwa, Phillip D Stricker, Riana Bornman, Weerachai Jaratlerdsiri, Vanessa M Hayes

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: prostate cancer; somatic alteration; epigenomics; epigenetic machinery; African ancestry; southern Africa; health disparity

Online: 26 May 2023 (07:24:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1867.v1

Frequency of Knee Pain and Risk Factors and Its Impact on Functional Impairment Among Saudi Adolescents

Ali H. Alyami, Hussam Darraj, Khalid M. Hakami, Faisal Hakami, Mohammed Awaf, Sulaiman Hamdi, Nawaf Bakri, Abdulaziz Saber, Almuhanad Alyami, Mohammed Khashab, Abdulaziz H. Alhazmi

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: knee pain; frequency of knee pain; exercise training; physical activity

Online: 26 May 2023 (05:38:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1864.v1

Perspectives and Implications of Coanda Effect in Aneurysms

Vicentiu Mircea Saceleanu, Razvan-Adrian Covache-Busuioc, Luca Andrei Glavan, Antonio Daniel Corlatescu, Alexandru Vlad Ciurea

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Coanda Effect; aneurysm; hemodynamics; Willis Polygon; pathology

Online: 26 May 2023 (05:26:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0713.v2

Vanadium Complexes with Thioanilide Derivatives of Amino Acids: Inhibition of Human Phosphatases and Specificity in Various Cell Models of Metabolic Disturbances

Grzegorz Kazek, Monika Głuch-Lutwin, Barbara Mordyl, Monika Kubacka, Anna Jurowska, Dariusz Cież, Bartosz Trzewik, Janusz Szklarzewicz, Monika A. Papież

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: vanadium complexes; phosphatases; in vitro; cells models; metabolic disorders; diabetes; insulin resistance; gluconeogenesis; NAFLD

Online: 26 May 2023 (05:19:48 CEST)

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Preprint INTERESTING IMAGES | doi:10.20944/preprints202305.1842.v1

Sequence of Rare Diagnoses in a Young Patient: Altitude Barotrauma Hemopneumothorax and Desquamative Interstitial Pneumonia

Iustina Leonte, Karina Ivanov, Angela Stefania Marghescu, Șerban-Radu Matache, Florica Valeria Negru, Ana Luiza Iorga, Silviu Mihail Dumitru, Beatrice Mahler

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: hemopneumothorax; altitude barotrauma; emphysematous lesions; desquamative interstitial pneumonia

Online: 26 May 2023 (05:14:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1855.v1

Molecular Mechanism in the Development of Pulmonary Fibrosis in Sarcoid Patient

Elisabetta Cocconcelli, Nicol Bernardinello, Gioele Castelli, Simone Petrarulo, Serena Bellani, Marina Saetta, Paolo Spagnolo, Elisabetta Balestro

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: sarcoidosis; advanced pulmonary fibrosis; signaling; molecular pathway

Online: 26 May 2023 (04:55:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1844.v1

Neuroprotective Potential of L-glutamate Transporters in Human Induced Pluripotent Stem Cell-Derived Neural Cells against Excitotoxicity

Kanako Takahashi, Yuto Ishibashi, Kaori Chujo, Ikurou Suzuki, Kaoru Sato

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: excitotoxicity; human induced pluripotent stem cell; neuron; astrocyte; L-glutamate transporter; EAAT1; EAAT2

Online: 26 May 2023 (04:22:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1839.v1

Side Chain-modified Benzothiazinone Derivatives with Anti-Mycobacterial Activity

Fan Dongguang, Bin Wang, Giovanni Stelitano, Karin Savková, Olga Riabova, Rui Shi, Xiaomei Wu, Laurent R. Chiarelli, Katarína Mikušová, Vadim Makarov, Yu Lu, Yuzhi Hong, Chunhua Qiao

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Anti-tubercular agents, DprE1 inhibitor, Structure activity relationship, in vivo activity

Online: 26 May 2023 (04:12:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1838.v1

Role of Selected Circulating Tumor Biomarkers in Patients with Skeletal Metastatic Pancreatic Neuroendocrine Neoplasms

Violetta Rosiek, Ksenia Janas, Magdalena Witkowska, Beata Kos-Kudła

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: pancreatic neuroendocrine neoplasms; bone metastasis; biomarker

Online: 26 May 2023 (04:11:51 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1835.v1

COVID-19 and COVID-19 Vaccine in Japan—A Review from a General Physician's Perspective

Hiroshi Kusunoki

Subject: Medicine And Pharmacology, Internal Medicine Keywords: COVID-19; SARS-CoV-2; vaccines; breakthrough infection; hybrid immunity; SARS-CoV-2 antibody

Online: 26 May 2023 (04:05:24 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1827.v1

Leveraging Data from Spontaneous Animal Disease Models to Streamline Pharmaceutical Research: A Tale of Two Species

Jonathan P Mochel, Karin Allenspach

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: One Health; Comparative Research; Reverse Translation

Online: 26 May 2023 (03:29:17 CEST)

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According to data from the U.S FDA, the likelihood of a new drug progressing from Phase I clinical trials to market approval is only 8% (DiMasi et al., 2016). Furthermore, a significant attrition rate of 35% occurs during Phase II studies due to the failure to demonstrate clinical efficacy (Arrowsmith, 2011). These statistics underscore the urgent need to reassess the current research and development (R&D) paradigm. To address this challenge, a promising approach called reverse translational pharmacology (RTP) has emerged, aiming to enhance the predictive value of preclinical models used in biomedical research by leveraging animal models that spontaneously develop analogous diseases to humans (Schneider et al., 2018). The rationale behind RTP is that knowledge gained from clinical trials in canines can subsequently inform and guide human clinical trials, leading to improved outcomes for both species. Additionally, this approach capitalizes on the opportunity to stimulate veterinary drug development by leveraging pharmacokinetic (PK), efficacy, and safety data obtained from human clinical studies (Schneider et al., 2018). The application of reverse translational pharmacology holds immense potential for expediting drug development and advancing medical knowledge. By employing animal models that naturally develop diseases akin to those found in humans, researchers can enhance the predictive capacity of preclinical models, thereby mitigating the high attrition rates encountered in later stages of clinical trials. Moreover, the exchange of knowledge and data between human and veterinary medicine engenders a synergistic relationship, fostering progress in both domains (Schneider et al., 2018). As this innovative approach gains momentum, fostering collaborations between basic and clinician scientists, pharmaceutical companies, and veterinary professionals becomes increasingly imperative. By sharing resources, expertise, and findings, we can pave the way for groundbreaking discoveries and the development of safe and efficacious treatments for diverse diseases. Embracing reverse translational pharmacology offers an avenue to redefine the R&D paradigm, bringing us closer to a future where a higher proportion of drugs successfully traverse the arduous path from the laboratory to market approval, ultimately benefiting patients across species. In this brief commentary, we highlight four examples of diseases where reverse translational modeling has been used to support pharmaceutical research, including some applications developed in our research laboratory (SMART Pharmacology) at the Iowa State University College of Veterinary Medicine. In doing so, this report also seeks to provide a brief overview of the heuristic value of computational approaches in comparative research. To further illustrate the value of these in silico approaches, we deliberately chose to employ some features of ChatGPT to produce the present summary (OpenAI. (2023). GPT-3.5 [ChatGPT]. Retrieved from https://openai.com).

Preprint ARTICLE | doi:10.20944/preprints202305.1817.v1

Auditory Processing in Musicians: a Basis for Auditory Training Optimization

Maria Kyrtsoudi, Christos Sidiras, Georgios Papadelis, Vasiliki Maria Iliadou

Subject: Medicine And Pharmacology, Otolaryngology Keywords: hearing; auditory processing; cognition; music; byzantine; percussion; rhythm

Online: 26 May 2023 (02:35:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1813.v1

Investigate the Effect of rh-BMP2 on the Healing Process of the Gingival Tissue using Ki-67 Marker

Mansour Chantiri, Toni Zeinoun

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: rh-BMP2; Ki67-Proliferation – Gingival Tissue

Online: 25 May 2023 (14:36:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1811.v1

Aortic Dilatation on the Edge of Dissection – Do We Operate Too Late? The Ratio between Ascending and Descending Aorta DiameteR (RADAR)

Nerea Lopez Perez, Philippe Reymond, Mustafa Cikirikcioglu, Mathieu Van Steenberghe, Tornike Sologashvili, Nicolas Murith, Thomas Perneger, Christoph Huber

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: cardiac surgery; ascending aorta dissection, ascending aorta aneurysm

Online: 25 May 2023 (13:00:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1805.v1

Recruitment of Knee Flexion to Compensate for Decompen-sated Spinopelvic Sagittal Balance

Jun Ouchida, Hiroaki Nakashima, Tokumi Kanemura, Kenyu Ito, Mikito Tsushima, Masaaki Machino, Sadayuki Ito, Naoki Segi, Yukihito Ode, Shiro Imagama

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: sagittal alignment; knee flexion; compensation parameter; spinopelvic sagittal balance; healthy subjects; compensation; Knee-spine syndrome

Online: 25 May 2023 (10:54:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1804.v1

Targeting M2-TAM via STAT3/NF-kB/AKT Signaling Pathway With Oxaliplatin, Retinoic Acid, and Libidibia ferrea -Loaded Extracellular Vesicles From Macrophages 1 Down-Regulates Murine Colon Cancer Metastasis

Thaís Gomes De Carvalho, Pablo Lara, Carla Jorquera-Cordero, Timo Schomann, Cícero Flávio Soares Aragão, Artur de Santana Oliveira, Vinicius Barreto Garcia, Shirley Vitória de Paiva Souza, Isabelle de Lima Marques, Luiz Alberto Lira Soares, Paulo Marcos Da Matta Guedes, Raimundo Fernandes De Araújo-Júnior

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: EVs; Macrophages 1; STAT3, AKT; NF-κB; metastasis; colorectal cancer.

Online: 25 May 2023 (10:48:17 CEST)

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Despite the numerous advances in target therapy for the treatment of colorectal cancer, aggressive colorectal cancer remains an incurable disease whose negative modulation of the immune system in the tumor microenvironment is still critical for improving the patient's prognosis. Extracellular vesicles (EVs) have received attention for their use as cell-membrane-camouflaged nanoparticles and drug delivery systems in nanomedicine derived by nearly all cell types for intercellular communication and regulation in the tumor microenvironment (TME). In this study, M1 Macrophage EVs (M1EVs) were used as nanocarriers of oxaliplatin (M1EV1) associated with retinoic acid (M1EV2) and Libidibia ferrea (M1EV3), or combined together with retinoic acid and Libidibia ferrea (M1EV4) to evaluate their antiproliferative and immunomodulatory potential on CT-26 and MC-38 colorectal cancer cell lines, as well as their capacity to prevent metastases in mouse models of colorectal cancer such as allographic and peritoneal. Tumors were evaluated by qRT-PCR and immunohistochemistry. The cell death profile and epithelial-mesenchymal transition process (EMT) were analyzed in vitro in colorectal cancer cell lines. Polarization of murine macrophages (RAW264.7 cells) was also carried out. M1EV2 and M1EV3 used alone or especially combined (M1EV4) downregulated the tumor progression by TME immunomodulation, leading to a decrease in primary tumor size and metastases in peritoneum, liver and lungs. STAT3, NF-kB and AKT were the major genes downregulated by systems of M1EVs. Tumor-associated macrophages (TAM) switched M2 phenotype (CD163) towards M1 (CD68) reducing levels of IL-10, TGF-β and CCL22. Furthermore, malignant cells showed overexpression of FADD, APAF-1, caspase-3, and E-cadherin, and decreased expression of MDR-1, survivin, vimentin, CXCR4, and PD-L1 after treatment with systems of M1EVs. The results obtained in this study provided evidence that EVs from M1 antitumor macrophages can transport drugs and increase their immunomodulatory and antitumor activity by stimulating activation or blockage of pathways involved in cell proliferation, migration, cell survival, and drug resistance processes.

Preprint BRIEF REPORT | doi:10.20944/preprints202305.1794.v1

Adjuvant Oligonucleotide Vaccine Increases Survival of Transgenic Mice [B6.Cg-Tg (K18-ACE2)2]

Volodymyr Oberemok, Kateryna Laikova, Kseniya Yurchenko, Ilya Novikov, Tatyana Makalish, Anatolii Kubyshkin, Oksana Andreeva, Anastasiya Bilyk

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: oligonucleotide vaccines; SARS-CoV-2; phosphorothioate oligonucleotides; innate immunity; adaptive immunity

Online: 25 May 2023 (10:13:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1784.v1

CSF Soluble TREM2 Concentrations Correlate With the Severity of Neurofibrillary Degeneration, Cognitive Impairment, and Inflammasome Activation in Alzheimer’s Disease

Ena Španić Popovački, Mirjana Babić Leko, Lea Langer Horvat, Klara Brgić, Željka Vogrinc, Marina Boban, Nataša Klepac, Fran Borovečki, Šimić Goran

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Alzheimer’s disease; cerebrospinal fluid; inflammasome; microglia; mild cognitive impairment; plasma; tau protein; TREM2

Online: 25 May 2023 (09:40:33 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202305.1778.v1

First Description of a Mixed Brugada Pattern in Inferior Leads

Alejandro Jesús Bermejo Valdés, Sara Iglesias Gutiérrez, Basilio Teja Ruiz

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Brugada Syndrome; channelopathy; arrhythmias

Online: 25 May 2023 (08:56:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1772.v1

The Beginner Laparoscopists Trends in the Learning Process Of Laparoscopy for Adnexal Gynecological Pathologies – The Experience of Our Center

Romina-Marina Sima, Anca-Violeta Crăițan, Liana Pleș, Florin Bobircă, Mihaela Amza, Gabriel-Petre Gorecki, Mihai-Teodor Georgescu, Bashar Haj Hamoud

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: learning process; laparoscopic gynecology; gynecological surgery; laparoscopy; ovarian cyst

Online: 25 May 2023 (08:45:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1766.v1

Hypoxic-Ischemic Brain Injury in ECMO: Pathophysiology, Neuromonitoring, and Therapeutic Opportunities

Shivalika Khanduja, Jiah Kim, Jin Kook Kang, Cheng-Yuan Feng, Melissa Ann Vogelsong, Romergryko G Geocadin, Glenn Whitman, Sung-Min Cho

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: hypoxia-ischemia brain injury; extracorporeal membrane oxygenation; neurological complication; neu-romonitoring; outcome.

Online: 25 May 2023 (08:26:02 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1761.v1

Checkpoint Inhibitors Induced Colitis: From Pathogenesis to Management

Maria Terrin, Giulia Migliorisi, Arianna Dal Buono, Roberto Gabbiadini, Elisabetta Mastrorocco, Alessandro Quadarella, Alessandro Repici, Armando Santoro, Alessandro Armuzzi

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: checkpoint inhibitors; colitis; immune-related adverse events; enterocolitis; diarrhea

Online: 25 May 2023 (08:03:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1753.v1

A Systematic Review and Bayesian Meta-Analysis of Medical Devices Used in Chronic Pain Management

Ashish Shetty, Gayathri Delanerolle, Chunli Deng, Heitor Cavalini, Xiaojie Yang, Anish Thillainathan, Yassine Bouchareb, Amy Boyd, Peter Phiri, Jian Shi

Subject: Medicine And Pharmacology, Internal Medicine Keywords: Chronic pain; Medical Devices; Neuromodulation; Clinical trials; Bayesian

Online: 25 May 2023 (07:27:31 CEST)

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Chronic non-cancer pain is a highly debilitating condition affecting approximately 20% of the global population. Chronic pain may lead to significant physical disability, emotional distress, social isolation and financial burden. Whilst^.pharmacological therapies remain the cornerstone of pain management in non-cancerous chronic pain, factors including the current opioid epidemic have led to non-pharmacological techniques becoming a more attractive proposition. We explored the prevalence of medical device use and their treatment efficacy in non-cancer pain management. A systematic methodology was developed, peer reviewed and published in PROSPERO (CRD42021235384). Key words of medical device, pain management devices, chronic pain, lower back pain, back pain, leg pain and chronic pelvic pain using Science direct, PubMed, Web of Science, PROSPERO, MEDLINE, EMBASE, PorQuest and ClinicalTrials.gov. All clinical trials, epidemiology and mixed methods studies that reported the use of medical devices for non-cancer chronic pain management published between the 1^st of January 1990 and the 30^th of April 2022 were included. 13 studies were included in systematic review, of these 6 were used in the meta-analysis with 173 participants. Our meta-analysis for pain reduction in each study showed that transcutaneous electrical nerve stimulation combined with instrument-assisted soft tissue mobilization treatment and pulsed electromagnetic therapy produced significant treatment on chronic lower back pain patients. Pooled evidence revealed the use of medical device related interventions resulted in 0.7 degree of pain reduction under a 0-10 scale. Significant improvement in disability scores, with a 7.44 degree reduction in disability level compared to a placebo using a 50 score range was also seen. The application of medical devices in patients with chronic pain has gained popularity due to increasingly cost effective techniques, minimally invasiveness and greater awareness of risks associated with pharmacological management. Our analysis has shown that the optimal use of medical devices in a sustainable manner requires further extensive research, needing larger cohort studies with greater gender parity, in a more diverse range of geographical locations.

Preprint ARTICLE | doi:10.20944/preprints202305.1738.v1

Fecal Microbiota and Associated Volatile Organic Compounds Distinguishing No Adenoma From High-Risk Colon Adenoma Adults

Kyriaki Katsaounou, Danae Yiannakou, Elpiniki Nikolaou, Cameron Brown, Paris Vogazianos, Aristos Aristodimou, Jianxiang Chi, Paul Costeas, Agapios Agapiou, Elisavet Frangou, George Tsiaoussis, George Potamitis, Athos Antoniades, Christos Shammas, Yiorgos Apidianakis

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Dysbacteriosis; pathobionts; nutrients; metabolites

Online: 25 May 2023 (05:15:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1737.v1

Oncological Outcomes after Laparoscopic and Open Liver Resection in patients with Colorectal Liver Metastases: A Single Centre Propensity Score Matched Study

Alberto Diaz-Garcia, Alex Gordon-Weeks, Robert Young, Timothy Gilbert, Adeeb Rehman, Hassan Malik, Stephen Fenwick, Robert P Jones, Rafael Diaz-Nieto

Subject: Medicine And Pharmacology, Surgery Keywords: liver surgery; colorectal cancer; laparoscopy; survival

Online: 25 May 2023 (05:14:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1725.v1

Molecular Insights into the Role of Gut Microbiota in Antibiotic Therapy Selection and Resistance Mitigation

Mihaela Andreescu

Subject: Medicine And Pharmacology, Hematology Keywords: Molecular biology; infectious diseases; clinical diagnostic; early detection; prognosis

Online: 25 May 2023 (03:34:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1721.v1

A Japanese Prospective, Multicenter Study of Colonic Stenting for Palliation Using a High Axial Force Self-Expandable Metal Stent for Malignant Large Bowel Obstruction in 200 Patients

Rei Ishibashi, Takashi Sasaki, Hiroyuki Isayama, Takeaki Matsuzawa, Toshio Kuwai, Tomonori Yamada, Shuji Saito, Masafumi Tomita, Toshiyasu Shiratori, Satoshi Ikeda, Hideki Kanazawa, Mitsuhiro Fujishiro, Iruru Maetani, Yoshihisa Saida

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: axial force; chemotherapy; malignant colorectal obstruction; palliation; self-expandable metal stent

Online: 25 May 2023 (03:11:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0930.v2

Economic Impact of the Implementation of a Multidisciplinary Diabetic Foot Clinic in a Tertiary Hospital

Alejandra Pérez-Montes, Laura Ricou Ríos, Arnau Pons Calsapeu, Francesc López Seguí, Clàudia Riera, Marc Sirvent, Esteban Reynaga, Jordi Viadé, Paloma Malagon, Berta Soldevila, Ricard Perez, Elena Casademunt, Glòria Bonet, Manel Puig Domingo, Oriol Estrada, Núria Alonso

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Multidisciplinary pain clinic; Diabetic foot; Amputation; Foot ulcer; Diabetes Mellitus; Economic evaluation; Cost Benefit Analysis

Online: 25 May 2023 (02:56:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1715.v1

Human Milk Feeding Could Protect Extremely Preterm Infants from Bronchopulmonary Dysplasia

Sergio Verd, Roser Porta, Gemma Ginovart, Alejandro Ávila Alvarez, Fermín García-Muñoz Rodrigo, Montserrat Izquierdo Renau, Paula Sol Ventura

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: bronchopulmonary dysplasia; extremely preterm infants; human milk; breastfeeding

Online: 25 May 2023 (02:28:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1713.v1

The Influence of Nicotine on Trophoblast-Derived Exosomes in a Mouse Model of Pathogenic Preeclampsia

Ayane Kubo, Keiichi Matsubara, Yuko Matsubara, Hirotomo Nakaoka, Takashi Sugiyama

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: preeclampsia; exosomes; nicotine; proteomics; bioinformatics

Online: 24 May 2023 (13:29:39 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1710.v1

Potential of Personalized Dendritic Cell-Based Immunohybridoma Vaccines to Treat Prostate Cancer

Simon Hawlina, Robert Zorec, Helena H. Chowdhury

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: prostate cancer; immunotherapy; dendritic cell-based vaccines; castration-resistant prostate cancer; tumor microenvironment; biomarkers

Online: 24 May 2023 (10:47:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1699.v1

Vitamin D Role in Childhood Mite Allergy and Allergen Immunotherapy (AIT)

Claudia Petrarca, Davide Viola

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Vitamin D; mite allergy; dermatophagoides; monomeric allergoid; immunotherapy; rhinitis; anti-histamine; Immunoglobulin; IgE; asthma; corticosteroid; children

Online: 24 May 2023 (05:14:28 CEST)

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The post-hoc analysis presented here aimed to address the influence of endogenous vitamin D in the immunological mechanism underlying effective mite allergoid-immunotherapy (AIT). Previously, we have shown that one subpopulation of T regulatory cells results in phenotypically identifiable as functionally potentiated and memory cells in allergic children after 12 months of this immunoactive treatment. Indeed, AIT is the only known treatment able to reshape the detrimental immune response against the allergen into a not noxious one. Besides, VD is widely considered an immunoregulatory molecule endogenously produced and exogenously provided with foods and supplements that might interact with AIT mechanism and affect its outcome. Therefore, a post-hoc analysis of the clinical and immunological data of three different cohorts of allergic patients was performed. One cohort (N=70) was on standard symptoms-controlling pharmacological treatment while the other two (N=60 and N=35, respectively) were treated with AIT for 12 months. Among the first were observed a lower mean endogenous VD level (<22 ng/ml) along with worse symptoms and higher use of medications. Remarkably, the comparison between two sub-cohorts of patients with serum VD above (N=32) or below (N=28) a cut-off value set at mean value (27 ng/ml) revealed that optimal improvement of all clinical and immune parameters was achieved (as expected by effective AIT), irrespective of the VD level. Notably, the third analysis, carried out on one cohort of AIT patients also taking concomitantly VD3 as food supplement (N=19), was distinguished by uppermost overall treatment outcome (amelioration of symptoms, lowest medications requirement, and reduction of total and allergen-specific IgE) as well as the increase of allergen-specific tolerogenic memory T regulatory cells. These findings suggest that endogenous VD level affects allergy severity and allergen immunotherapy effectiveness. Also, VD3 might be investigated as an add-on supplement to get the best out of immunotherapy in VD deficient/insufficient allergic patients. The immunogenic but low-allergenic mite allergoid used as bioactive agent might have contributed to minimize allergic and highlight immunological effects described here.

Preprint COMMUNICATION | doi:10.20944/preprints202305.1694.v1

Synthetic Data & the Future of Women's Health: A Synergistic Relationship

Gayathri Delanerolle, Peter Phiri, Heitor Cavalini, David Benfield, Ashish Shetty, Yassine Bouchareb, Jian Shi, Alain Zemkoho

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Womens Health; Data Science; Data Methods; Artificial Intelligence

Online: 24 May 2023 (04:48:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1684.v1

Reference Values for the Grocery Shelving Test among United Arab Emirates Population: A Cross Sectional Study

Gopala Krishna Alaparthi, Aishwarya Prabhakar Gatty, Huda Isa, Latifa Hassan, Noura Khalid, Abdullah Osman, Sara Mahmoud, May Tamim, Kalyana Chakravarthy Bairapareddy, Arthur De Sa Ferreira

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: Arm elevation; Grocery Shelving Test; Functional assessment; Reference values; Upper limb activities

Online: 24 May 2023 (04:13:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1680.v1

Pentraxin 3 in Kidney Disease and Renal Replacement Therapies

Vincenzo Losappio, Infante Barbara, Annamaria Maiorano, Serena Simiele, Sara Rosato, Silvia Mercuri, Dario Troise, Giovanni Stallone, Paola Pontrelli

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Chronic kidney disease; Pentraxin-3; Renal Replacement Therapies; Inflammaging.; innate immunity

Online: 24 May 2023 (03:59:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1679.v1

Ferroptosis-Mediated Cell Death Induced by NCX4040, The Non-Steroidal Nitric Oxide Donor, in Human Colorectal Cancer Cells: Implications in Therapy

Birandra Kumar Sinha, Carl D Bortner, Alan K Jatmusch, Erik J Tokar, Carri Murphey, Xian Wu, Heather Winter, Ronald E Cannon

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Ferroptosis; NCX4040; Erastin; Ferrostatin-1; RSL3; Colon Cancer

Online: 24 May 2023 (03:52:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1675.v1

Development of an Antiviral Ion-Activated In Situ Gel Containing 18β-Glycyrrhetinic Acid: A Promising Alternative Against Respiratory Syncytial Virus

Burcu Özkan, Ebru Altuntaş, Ümmühan Ünlü, Hasan Hüseyin Doğan, Yıldız Özsoy, Rabia Çakır Koç

Subject: Medicine And Pharmacology, Pharmacy Keywords: in situ gel; intranasal delivery, gellan gum; mucoadhesion; 18β-glycyrrhetinic acid; respiratory syncytial virus; common cold; antiviral activity

Online: 24 May 2023 (03:08:42 CEST)

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The human respiratory syncytial virus (hRSV) poses a serious risk to global public health and is a significant cause of severe lower respiratory infections. There are currently only a few therapies available to treat RSV infections, as well as no RSV vaccinations. Therefore, there is an urgent demand for clinically feasible, safe, and affordable RSV prevention and treatment alternatives. In this study, an ion-activated in situ gel comprising broad-spectrum antiviral 18β-glycyrrhetinic acid (GA) for antiviral action on RSV was developed. In this content, mechanical properties, sprayability, drug content, pH, ex vivo mucoadhesive strength, in vitro drug release pattern, and stability were all examined. Rheological properties were also tested by utilizing in vitro gelation capacity and rheological synergism tests. Finally, the optimized in situ gel's cytotoxic and antiviral activities on RSV cultured in human laryngeal epidermoid carcinoma (HEP-2) cell line were evaluated. In conclusion, the optimized in situ gel prepared with a combination of 0.5%w/w gellan gum and 0.5%w/w sodium carboxymethylcellulose demonstrated good gelation capacity and sprayability (weight deviation between T0 and T14: 0.34%), desired rheological synergism (mucoadhesive force (Fb): 9.53), mechanical characteristics (adhesiveness: 0.300 mJ ± 0.05), ex vivo bioadhesion force (19.67 g ± 1.90), drug content uniformity (RSD%: 0.494), and sustained drug release over period of 168 h (101.57% ± 0.53). The antiviral activity test results showed that the optimized in situ gel had strong anti-HRSV activity (EC50 simultaneous = 0.05 µg/ml, SI = 306; EC50 preinfection = 0.154 µg/ml, SI= 100) which was significantly higher than that of ribavirin (EC50 =4.189 µg/ml; SI= 28) used as a positive control against hRSV. In conclusion, this research has demonstrated the formulation of an effective antiviral nasal spray that has the ability to possess both prophylactic and virucidal activity.

Preprint ARTICLE | doi:10.20944/preprints202305.1674.v1

An Ethyl Acetate Extract of Eryngium carlinae Inflorescences Attenuates Oxidative Stress and Inflammation in the Liver of Streptozotocin-Induced Diabetic Rats

Cristian M. Trejo-Hurtado, Cinthia I. Landa-Moreno, Jenaro Lemus-De la Cruz, Donovan J. Peña-Montes, Rocío Montoya-Pérez, Rafael Salgado-Garciglia, Salvador Manzo-Avalos, Christian Cortés-Rojo, Juan Luis Monribot-Villanueva, José Antonio Guerrero-Analco, Alfredo Saavedra-Molina

Subject: Medicine And Pharmacology, Complementary And Alternative Medicine Keywords: antioxidants; anti-inflammatory activity; diabetes mellitus; Eryngium carlinae; phenolic compounds; rosmarinic acid

Online: 24 May 2023 (03:06:23 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202305.1667.v1

Liphedema Rehabilitation after Breast Cancer Surgery Using Combined Physiotherapy Techniques

Maria Baltag Oana, Mihaela Apostu, Kristo Xhardo, Daniel Andrei Iordan, Ilie Onu, Mariana Cordun

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: breast cancer; lymphedema management; manual lymph drainage; liphedema rehabilitation after mastectomy

Online: 24 May 2023 (02:11:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1666.v1

Minimal Access Coronary Revascularization: Past, Present and Future

Rushmi Purmessur, Jason Ali

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: minimal access; coronary artery; revascularisation; MIDCAB; TECAB; HCR

Online: 24 May 2023 (02:04:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1664.v1

Diagnosis and Management of Severe Water-Related Skin and Soft Tissue Sepsis: A Summative Review of the Literature

Shanisa Naidoo, Arnold M Zwane, Ahmed Paruk, Timothy Craig Hardcastle

Subject: Medicine And Pharmacology, Surgery Keywords: Soft tissue; necrotizing infection; water borne; vibrio; fungal; aeromonas; debridement; negative pressure wound care

Online: 23 May 2023 (14:43:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1657.v1

Metabolic Dysregulation Explains the Diverse Impacts of Obesity in Males and Females with Gastrointestinal Cancers

Spencer R. Rosario, Bowen Dong, Yali Zhang, Hua-Hsin Hsiao, Emily Isenhart, Jianmin Wang, Erin M. Siegel, Arta Monir Monjazeb, Dwight H. Owen, Prasenjit Dey, Fred K. Tabung, Daniel Spakowicz, William J. Murphy, Stephen B. Edge, Sai Yendamuri, Sami Ibrahimi, Jill M. Kolesar, Patsy H. McDonald, Deepak Vadehra, Michelle Churchman, Song Liu, Pawel Kalinsky, Sarbajit Mukherjee

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: obesity; gender; metabolism; cancer; gastrointestinal

Online: 23 May 2023 (12:43:10 CEST)

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Background: The prevalence of obesity, defined as body mass index (BMI) ≥ 30 kg/m2, has reached epidemic levels. Obesity is associated with an increased risk of various cancers, including gastrointestinal ones. Recent evidence has suggested that obesity disproportionately impacts males and females with cancer, resulting in varied transcriptional and metabolic dysregulation. Methods: This study aimed to elucidate the differences in the metabolic milieu of adenocarcinomas of the gastrointestinal (GI) track both related and unrelated to sex in obesity. To demonstrate these obesity and sex-related effects, we utilized three primary data sources: serum metabolomics from obese and non-obese patients assessed via the Biocrates MxP Quant 500 mass spectrometry-based kit, the ORIEN tumor RNA-sequencing data for all adenocarcinoma cases to assess the impacts of obesity, and publicly available TCGA transcriptional analysis to assess GI cancers and sex-related differences in GI cancers specifically. We applied and integrated our unique transcriptional metabolic pipeline in combination with our metabolomics data to reveal how obesity and sex can dictate differential metabolism in patients. Results: Differentially expressed genes (DEG) analysis of ORIEN obese adenocarcinoma as compared to normal-weight adenocarcinoma patients resulted in large-scale transcriptional reprogramming (4029 DEGs, adj. p < 0.05 and |logFC|>0.58). Gene Set Enrichment and metabolic pipeline analysis showed genes enriched for pathways relating to immunity (inflammation, and CD40 signaling, among others) and metabolism. Specifically, we found alterations to steroid metabolism and tryptophan/kynurenine metabolism in obese patients, both of which are highly associated with disease severity and immune cell dysfunction. These findings were further confirmed using the TCGA colorectal adenocarcinoma (CRC) and esophageal adenocarcinoma (ESCA) data, which showed similar patterns of increased tryptophan catabolism for kynurenine production in obese patients. These patients further showed disparate alterations between males and females when comparing obese to non-obese patient populations. Alterations to immune and metabolic pathways were validated in 6 patients (2 obese and 4 normal weight) via CD8+/CD4+ peripheral blood mononuclear cell RNA-sequencing and paired serum metabolomics, which showed differential kynurenine and lipid metabolism, which corresponded with altered T-cell transcriptome in obese populations. Conclusions: Overall, obesity is associated with differential transcriptional and metabolic program in various disease sites. Further, these alterations, like kynurenine and tryptophan metabolism, which impact both metabolism and immune phenotype, vary with sex and obesity together. This study warrants further in-depth investigation into obesity and sex-related alterations in cancers that may better define biomarkers of response to immunotherapy.

Preprint REVIEW | doi:10.20944/preprints202305.1646.v1

The Exploitation of Lysosomes in Cancer Therapy with Graphene-Based Nanomaterials

Biljana Ristic, Mihajlo Bosnjak, Maja Misirkic Marjanovic, Danijela Stevanovic, Kristina Janjetovic, Ljubica Harhaji-Trajkovic

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: graphene-based nanomaterials; graphene-based drug delivery systems; lysosomes; cancer; endosomal/lysosomal escape; lysosomal cell death

Online: 23 May 2023 (10:52:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1641.v1

Extensive Drug Resistance of Strong Biofilm-Producing Acinetobacter baumannii Strains Isolated from Infections and Colonization Hospitalized Patients in Southern Poland

Tomasz Kasperski, Dorota Romaniszyn, Estera Jachowicz-Matczak, Monika Pomorska-Wesołowska, Jadwiga Wójkowska-Mach, Agnieszka Chmielarczyk

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Acinetobacter baumannii; biofilm; extensively drug resistance; biofilm associated genes

Online: 23 May 2023 (10:33:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1639.v1

Hydrogen Sulfide Ameliorates SARS-CoV-2–Associated Lung Endothelial Barrier Disruption

Olivier Escaffre, Peter Szaniszlo, Gabor Törő, Caitlyn L. Villas, Brenna J. Servantes, Ernesto Lopez, Terry L. Juelich, Corri B. Levine, Susan L. F. McLellan, Jessica C. Cardenas, Alexander N. Freiberg, Katalin Módis

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: endothelial barrier; COVID-19; SARS-CoV-2; hydrogen sulfide; cytokine; TNF-α

Online: 23 May 2023 (10:31:05 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1637.v1

Computational Advancements in Drug Repurposing for Cancer Combination Therapy Prediction

Victoria L. Flanary, Jennifer L. Fisher, Elizabeth J. Wilk, Timothy C. Howton, Brittany N. Lasseigne

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: computational; drug repurposing; drug repositioning; cancer; combination therapy; network biology; machine learning; deep learning; precision oncology

Online: 23 May 2023 (10:19:47 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1633.v1

Pathology: One Score Fits All?

Vincenzo Villanacci, Rachele Del Sordo, Tommaso Lorenzo Parigi, Giuseppe Leoncini, Gabrio Bassotti

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: ulcerative colitis; Crohn’s disease; scoring system; histological mucosal healing

Online: 23 May 2023 (09:51:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1615.v1

Restoring Homeostasis as a Promising Therapeutic Strategy for Obesity and Sleep Disorders

Vijay Kumar, Shampa Ghosh, Pradeep Kumar Mishra, Areeba Aziz, Rachna Mehta, Jitendra Kumar Sinha

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Anti-obesity drugs; BMI; Glucose tolerance; Homeostasis; Obesity; Sleep disturbances; Molecular pathology

Online: 23 May 2023 (08:15:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1605.v1

Development and Evaluation of Apocynin Loaded pH-sensitive Nanoparticle Based Hydrogel for the Management of Rheumatoid Arthritis

Rasikha Akbar, Dildar Khan, Muhammad Bilal Sabir, Irfan Shaukat, Irfa Basharat Rajpoot, Asim Ur Rehman, Naz Dilawar, Shefaat Ullah Shah, Asif Nawaz, Faiqa Falak Naz, Saima Mehmood, Hafiz Muhammad Basit, Suliman Yousef Alomar, Kifayat Ullah Shah

Subject: Medicine And Pharmacology, Pharmacy Keywords: pH sensitive Polymer; Methacrylate Polymers; Eudragit L100; Polymeric nanoparticles; Apocynin; Rheumatoid arthritis; Carbopol-934 based Hydrogel; Transdermal Drug Delivery System

Online: 23 May 2023 (07:51:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1603.v1

Cryptococcal Infection Is the Leading Cause of Death among HIV-Infected Sepsis Patients in Central Ethiopia

Tafese Beyene Tufa, Hans Martin Orth, Tobias Wienemann, Björn-Erik Ole Jensen, Colin R Mackenzie, David R Boulware, Tom Luedde, Fedlt Torsten

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Cryptococcal infection; Sepsis; qSOFA; blood culture; HIV; Africa

Online: 23 May 2023 (07:47:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1601.v1

The Bidirectional Association Between Periodontitis and COVID-19: A Review of Current Evidence

Miriam Ting, Rodrigo Neiva, Leenus Tafline, Jon B Suzuki

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: COVID-19; SARS-CoV-2; Periodontitis; Herpesviruses; Periodontal; Biomarkers

Online: 23 May 2023 (07:43:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1584.v1

Prospective Comparative Analysis of Zero-Heat-Flux Thermometer (SpotOn®) Compared with Tympanic Thermometer and Bladder Thermometer in Extremely Aged Patients Undergoing Lower Extremity Orthopedic Surgery

Chunghyun Park, Taeyeun Kim, Soojeong Oh, Yun-Sic Bang

Subject: Medicine And Pharmacology, Anesthesiology And Pain Medicine Keywords: Body temperature; Elderly patient; Orthopedic surgery; Zero-Heat-Flux thermometer

Online: 23 May 2023 (05:32:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0132.v2

Sentinel Lymph Node in Breast Cancer: An Innovative Technique Innovation in Breast Cancer

Paolo Izzo, Claudia De Intinis, Simone Sibio, Luigi Basso, Andrea Polistena, Gabriele Raimondo, Massimo Codacci-Pisanelli, Luciano Izzo, Sara Izzo

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: SLNB; SPIO; ALND; LS; superparamagnetic iron oxide nanoparticles

Online: 23 May 2023 (05:26:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1576.v1

Potential Utility of a Panel of Four-miRNAs As Biomarkers in the Clinical Management of Obese and Non-obese Colorectal Cancer Patients

Sofía Elena Tesolato, Daniel González-Gamo, Ana Barabash, Paula Claver, Sofía Cristina De la Serna, Inmaculada Domínguez-Serrano, Jana Dziakova, Carmen De Juan, Antonio José Torres, Pilar Iniesta

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: colorectal cancer; adipose tissue; miRNAs; serum biomarkers; obesity

Online: 23 May 2023 (05:03:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1575.v1

Application of Heisenberg’s Uncertainty Principle in the Diag-Nostic Dilemma of Children With Acute Appendicitis: Negative Appendectomy Versus Complicated Appendicitis Rates

Stylianos Roupakias, Sotirios Fouzas, Eirini Kostopoulou, Despoina Gkentzi, Theodore Dassios, Ioannis Spyridakis, Xenophon Sinopidis

Subject: Medicine And Pharmacology, Surgery Keywords: complicated appendicitis; negative appendectomy; Heisenberg’s principle; diagnostic uncertainty; children

Online: 23 May 2023 (04:58:36 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1574.v1

Personalized Medicine of Omega-3 Fatty Acids in Depression Treatment in Obese and Metabolically Dysregulated Patients

Suet-Kei Wu, Wei-Jen Chen, Jane Pei-Chen Chang, Ta-Wei Guu, Ming-Che Hsin, Chih-Kun Huang, David Mischoulon, Lucile Capuron, Kuan-Pin Su

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: omega-3 polyunsaturated fatty acids (n-3 PUFAs); major depressive disorder (MDD); obesity; metabolic dysregulation; comorbidities

Online: 23 May 2023 (04:54:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1572.v1

The Prognostic Nutritional Index (PNI): A New Biomarker for Determining Prognosis in Metastatic Castration-Sensitive Prostate Carcinoma

Halil İbrahim Ellez, Merve Keskinkilic, Huseyin Salih Semiz, Mehmet Emin Arayici, Erdem Kısa, Ilhan Oztop

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: prostate cancer; prognostic nutritional index; inflammation; prognosis

Online: 23 May 2023 (04:50:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1570.v1

The role of w3 PUFFA in Patients with Metabolic Syndrome and Endothelial Dysfunction

Monika Jankajova, Dominik Pella, Pavol Zenuch, Zuzana Zenuchova, Stefan Toth, Peter Kalanin, Jan Fedacko

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: metabolic syndrome; endothelial dysfunction; omega-3 fatty acid

Online: 23 May 2023 (04:45:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1559.v1

Educational Intervention Effects on Depression and Anxiety in Patients After Myocardial Infarction: A Randomized Controlled Trial

Lazzat M. Zhamaliyeva, Damira G. Zhamankulova, Nurgul A. Abenova, Gulbakit K. Koshmaganbetova

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: anxiety; cardiac rehabilitation; depression; medical education; primary care

Online: 23 May 2023 (04:16:09 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1557.v1

Conducting and Reporting Meta-analysis: A Checklist for Reviewers

SAMIT GHOSAL

Subject: Medicine And Pharmacology, Internal Medicine Keywords: Meta-analysis; heterogeneity; reviewer; checklist

Online: 23 May 2023 (04:09:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1549.v1

The Heterogeneous Progressive Multiple Sclerosis Lesion. How Can We Assess and Modify a Degenerating Lesion?

Olivia Ellen, Sining Ye, Danica Nheu, Mary Dass, Maurice Pagnin, Ezgi Ozturk, Paschalis Theotokis, Nikolaos Grigoriadis, Steven Petratos

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: multiple sclerosis; oligodendrocyte; myelination; inflammation; microglia; macrophage; bi-omarkers; imaging diagnostics

Online: 23 May 2023 (03:39:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1536.v1

Evolution of Collective Sars-Cov-2 Humoral Immunity among the Population of the Republic of Belarus

Anna Y. Popova, Viacheslav Smirnov, Svetlana A. Egorova, Alexander A. Tarasenko, Alla M. Dashkevich, Angelika M. Milichkima, Angela L. Scuranovich, Irina V. Drozd, Iruna N. Glinskaya, Elena V. Zueva, Elena O. Samoilovich, Valery A. Ivanov, Edward S. Ramsay, Alexandra V. Gubanova, Victoria G. Drobyshevskaya, Oyuna B. Zhumbaeva, Olga A. Petrova, Alexandra P. Razumovskaya, Inna A. Karaban, Tamara V. Amvrosieva, Natalya P. Shmeleva, Ludmila V. Rubanic, Alina M. Dronina, Areg A. Totolian

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Republic of Belarus; population; SARS-CoV-2; COVID-19; seromonitoring; herd immunity; antibodies; nucleocapsid; receptor binding domain; vaccination; hybrid immunity

Online: 23 May 2023 (02:47:10 CEST)

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Background. The course of the COVID-19 epidemic process depends on population immunity which prevents pathogen spread among the population. Aim: to study the evolution of SARS-CoV-2 humoral immunity in the Belarusian population relative to COVID-19 pandemic dynamics. Materials and methods. The work was carried out according to a methodology for assessing population immunity developed by Rospotrebnadzor (Russia) and the Belarusian Ministry of Health with the participation of the St. Petersburg Pasteur Institute (SPPI), taking into account WHO recommendations. The study was approved by the Bioethics Committee of Belarus and the SPPI Bioethics Committee. Participant selection was carried out by questionnaire using a cloud (internet server) service. To monitor population immunity, a cohort of 4,661 people (participating in all stages of seromonitoring) was formed from the overall volunteer group. Volunteers were randomized by age group (1-17, 18-29, 30-39, 40-49, 50-59, 60-69, 70+ years), region, and professional group. For the detection of antibodies (Abs) to nucleocapsid (Nc) and S glycoprotein receptor-binding domain (RBD), corresponding assay systems were used following manufacturer instructions. The study was conducted in 4 stages according to a single scheme. Results. In the 1st stage (pandemic month 15), collective immunity was due mainly to Nc+RBD+ Ab status alone. By the 2nd stage (carried out after 4 months), their share decreased 1.2-fold, while the share of volunteers who had only RBD Abs increased 1.7-fold. In the 3rd and 4th stages (carried out after 9 and 19 months), the share of persons with RBD+Nc‒ compared to the 2nd stage decreased by 3.5%; the proportion of persons with Nc+RBD‒ Abs increased by 1.5-fold. The most important factor in population immunity was vaccination of the population, the coverage of which reached 70% by the 4th stage. Among vaccines, the Sputnik V and Sputnik Light vector designs were used most often. The whole-virion, inactivated BIBP-CorV vaccine was used less often. Conclusion. The evolution of collective SARS-CoV-2 humoral immunity included a set of changes in circulating Ab levels (Nc, RBD). The hybrid immunity formed helped to reduce the incidence to nearly zero.

Preprint ARTICLE | doi:10.20944/preprints202305.1533.v1

Associations of Clinical and Dosimetric Parameters with Urinary Toxicities after Prostate Brachytherapy: A Long-Term Single-Institution Experience

Masaya Ito, Chiyoko Makita, Takayuki Mori, Hirota Takano, Tomoyasu Kumano, Masayuki Matsuo, Koji Iinuma, Makoto Kawase, Keita Nakane, Masahiro Nakano, Tomoyasu Kumano

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: prostate cancer; brachytherapy; lower urinary tract symptom; international prostate symptom score (IPSS); overactive bladder symptom score (OABSS)

Online: 22 May 2023 (16:28:32 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0843.v2

Zanubrutinib: Coming of Age for BTK Inhibitors in the Treatment of Chronic Lymphocytic Leukemia?

Stefano Molica, Constantine Tam, David Allsup, Aaron Polliack

Subject: Medicine And Pharmacology, Hematology Keywords: CLL, Zanubrutinib, BTK inhibitors, efficacy, safety, chronic lymphocytic leukemia

Online: 22 May 2023 (16:15:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1527.v1

Evaluation of the Effects of Different Polishing Protocols on the Surface Characterizations of 3D-Printed Acrylic Denture Base Resins

Yousif A. A. Al-Dulaijan

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: Additive manufacturing; Chairside kit; SEM; Surface roughness; Rapid prototype

Online: 22 May 2023 (12:40:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1518.v1

Comparative Analysis of Maternal and Neonatal Outcomes between Elective and Emergency Cesarean Section at a Single Tertiary Hospital

Nihal Al Riyami, Shurooq Al Shereiqi, Silja Pillai

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: Cesarean section; risk factors; pregnancy complications; pregnancy outcome

Online: 22 May 2023 (11:21:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1516.v1

Association Between Low-density Lipoprotein Cholesterol and Vascular Biomarkers in Primary Prevention

Michaela Kozakova, Carmela Morizzo, Giuli Jamagidze, Daniele Della Latta, Sara Chiappino, Dante Chiappino, Carlo Palombo

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: lipid-lowering treatment; primary prevention; coronary calcium; intima-media thickness; arterial stiffness; carotid artery; femoral artery

Online: 22 May 2023 (11:19:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1174.v2

In Vitro and In Silico Analysis of the Anti-cancer Effects of Eurycomanone and Eurycomalactone from Eurycoma longifolia.

Nurhanan Murni Yunos, Habibah A Wahab, Mohammad G. Al-Thiabat, Nor Jannah Sallehudin, Muhamad Haffiz Jauri

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Eurycoma longifolia; quassinoids; cancer cell lines; apoptosis; TNF-α; DHFR; molecular docking; ADMET.

Online: 22 May 2023 (11:02:09 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1507.v1

Clinical Advances and Perspectives in Targeted Radionuclide Therapy

Nicolas Lepareur, Barthélémy Ramée, Marie Mougin-Degraef, Mickaël Bourgeois

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Radionuclide therapy; nuclear medicine; radiopharmaceutical; antibody; peptide; small molecule inhibitor; tumor; microenvironment

Online: 22 May 2023 (10:51:48 CEST)

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Preprint INTERESTING IMAGES | doi:10.20944/preprints202305.1509.v1

Atypical Prostatic Stromal Sarcoma Presented with T2WI Central Heterogeneous Hyperintense Lesion Showing Peripheral Zone & Peri Prostatic Fat Invasion

Jehan Abdelghany Mazroa, Khalid A Almakhalas, Galal A Bashanfer

Subject: Medicine And Pharmacology, Other Keywords: prostatic carcinoma; MRI prostate; MRI prostate stromal sarcoma

Online: 22 May 2023 (10:51:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1505.v1

Impact of Adherence to the Mediterranean Diet on Health-Related Quality of Life in Poorly Controlled Diabetics

Carmen Celada Roldan, Jaime Lopez Diez, Faustino Rider Garrido, Maria Angeles Cerezuela Abarca, Almudena Tarraga Marcos, Pedro Tarraga Lopez

Subject: Medicine And Pharmacology, Other Keywords: Quality of life; Mediterranean diet; , MEDAS-14; type 2 diabetes; physical dimension; emotional dimension

Online: 22 May 2023 (10:47:21 CEST)

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Introduction: When analyzing the current situation in Spain, derived from our current lifestyles far from following a Mediterranean lifestyle, there is an alarming prevalence of diabesity, which increases the global risk of suffering from cardiovascular disease (CVD) and decreases the quality of life related to health (QLRH). In order to guarantee, beyond glycemic control of patients with type 2 diabetes (DM2), adequate control of cardiovascular risk factors (CVRF) in DM2, it will be necessary to carry out a community intervention focused on the application of education programs focused on promoting the acquisition of healthy eating habits through the Mediterranean diet (DMED). This dietary pattern, together with physical exercise, has been shown to contribute to improving the QLRH of patients. Objective: To analyze the quality of life related to health, present in patients with poorly controlled type 2 diabetes, to determine the possible relationship between this and the degree of adherence to the Mediterranean diet and to examine whether there are differences between the sexes. Material and methods: Observational descriptive study in 93 patients diagnosed with DM2 with poor glycemic control (1Ac ≥ 7%), carried out in various health centers in Albacete and Cuenca, in which the baseline relationship between adherence to DMed and the HRQoL. They were administered a data collection sheet that included a survey of the degree of adherence to the DMed (MEDAS-14) and QLRH (SF-12v2) in the Primary Care (PC) medical and nursing consultations. The variables were analyzed: age groups, sex, years of evolution of DM2, body mass index (BMI), as well as basal glycemia (GB) and glycosylated hemoglobin (HbA1c). The “MEDAS-14” (adherence to DMED) was the main variable and the “SF-12v2” (QLRH) was the secondary variable. Material and methods: Observational descriptive study in 93 patients diagnosed with DM2 with poor glycemic control (1Ac ≥ 7%), carried out in various health centers in Albacete and Cuenca, in which the baseline relationship between adherence to DMed and the HRQoL. They were administered a data collection sheet that included a survey of the degree of adherence to the DMed (MEDAS-14) and QLRH (SF-12v2) in the Primary Care (PC) medical and nursing consultations. The variables were analyzed: age groups, sex, years of evolution of DM2, body mass index (BMI), as well as basal glycemia (GB) and glycosylated hemoglobin (HbA1c). The “MEDAS-14” (adherence to DMED) was the main variable and the “SF-12v2” (QLRH) was the secondary variable. Results: Patients with poorly controlled DM2 and with low adherence to the MedDM show a non-significantly greater affectation in the physical sphere: fair general state of health, physical function I (limitation to make moderate efforts such as moving a table, vacuuming or walk more than an hour), physical function II (limitation to climb several flights of stairs), physical role (problems at work or daily activities doing less than loved ones), physical role II (they had to stop performing some tasks at work or activities of daily living) and regular body pain. Presenting less affectation in the mental field: emotional role I, emotional role II, mental health I, vitality and mental health II. Without affectation in the social function since they consider that rarely the emotional or physical problems have hindered their social activities. Conclusions: Diabetic patients with poor glycemic control have low adherence to the MedMD (<9 points) and have poor QLRH. Low adherence to the DMed and obesity are related to a greater affectation in the physical dimension and less affectation in the mental dimension without affectation in the "social function".

Preprint REVIEW | doi:10.20944/preprints202305.1503.v1

Exosomes Analysis in Prostate Cancer: How They Can Improve Biomarkers Performance

Stefano Salciccia, Marco Frisenda, Giulio Bevilacqua, Luca Gobbi, Bruno Bucca, Martina Moriconi, Pietro Viscuso, Alessandro Gentilucci, Gianna Mariotti, Susanna Cattarino, Flavio Forte, Stefano Fais, Mariantonia Logozzi, Beatrice Sciarra, Alessandro Sciarra

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: exosome; prostate cancer; biomarker

Online: 22 May 2023 (10:39:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1500.v1

Autologous Transplantation May Still Effectively Treat Relapsed Diffuse Large B-cell Lymphoma in Selected Patients

Reka Rahel Bicsko, Lili Antal, Ferenc Magyari, Róbert Szász, Miklós Udvardy, Arpad Illes, Lajos Gergely

Subject: Medicine And Pharmacology, Hematology Keywords: diffuse large B-cell lymphoma; relapsed; refractory; autologous stem cell transplantation

Online: 22 May 2023 (10:29:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1499.v1

Chemoradiation with Cisplatin vs. Carboplatin for Squamous Cell Carcinoma of the Head-and-Neck (SCCHN)

Dirk Rades, Inga Zwaan, Tamer Soror, Christian Idel, Ralph Pries, Karl L Bruchhage, Samer G. Hakim, Nathan Y. Yu

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: head-and-neck cancer; chemoradiation; cisplatin; carboplatin; loco-regional control; metasta-ses-free survival; overall survival; toxicity

Online: 22 May 2023 (10:21:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1489.v1

Clinical Impact of Systematic Assessment and Psychoeducation in Specialized Treatment of Adolescents with Severe Functional Somatic Disorders: Results from the AHEAD Study

Karen Hansen Kallesøe, Kaare Bro Wellnitz, Eva Ørnbøl, Charlotte Ulrikka Rask

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: Adolescents; Assessment; Functional Somatic Disorders; Functional Somatic Syndromes; Psychoeducation

Online: 22 May 2023 (09:44:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1487.v1

Laboratory Findings and Biomarkers in Long COVID: What Do We Know So Far? Insights Into Epidemiology, Pathogenesis, Therapeutic Perspectives and Challenges

Dimitrios Tsilingiris, Natalia G. Vallianou, Irene Karampela, Gerasimos Socrates Christodoulatos, Georgios Papavasileiou, Dimitra Petropoulou, Faidon Magkos, Maria Dalamaga

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: biomarkers; COVID-19; epidemiology; laboratory; long COVID; pathogenesis; post-acute sequelae of SARS-CoV-19 infection (PASC); post COVID; post-COVID syndrome (PCS)

Online: 22 May 2023 (09:41:11 CEST)

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Long COVID (LC) encompasses a constellation of long-term symptoms experienced by at least 10% of people after the initial SARS-CoV-2 infection, and so far has affected about 65 million people. The etiology of LC remains unclear; however, many pathophysiological pathways may be involved, including viral persistence; chronic, low grade inflammatory response; immune dysregulation and defective immune response; reactivation of latent viruses; autoimmunity; persistent endothelial dysfunction and coagulopathy; gut dysbiosis; hormonal dysregulation, mitochondrial dysfunction; and autonomic nervous system dysfunction. There are no specific tests for the diagnosis of LC, and clinical features including laboratory findings and biomarkers may not specifically relate to LC. Therefore, it is of paramount importance to develop and validate biomarkers that can be employed for the prediction, diagnosis and prognosis of LC and its therapeutic response. Promising candidate biomarkers that are found in some patients are markers of systemic inflammation including acute phase proteins, cytokines and chemokines; biomarkers reflecting SARS-CoV-2 persistence, reactivation of herpesviruses and immune dysregulation; biomarkers of endotheliopathy, coagulation and fibrinolysis; microbiota alterations; diverse proteins and metabolites; hormonal and metabolic biomarkers; as well as cerebrospinal fluid biomarkers. At present, there are only two reviews summarizing relevant biomarkers; however, they do not cover the entire umbrella of current biomarkers or their link to etiopathogenetic mechanisms, and the diagnostic work-up in a comprehensive manner. Herein, we aim to appraise and synopsize the available evidence on the typical laboratory manifestations and candidate biomarkers of LC, their classification based on main LC symptomatology in the frame of the epidemiological and pathogenetic aspects of the syndrome, and furthermore assess limitations and challenges as well as potential implications in candidate therapeutic interventions.

Preprint ARTICLE | doi:10.20944/preprints202305.1477.v1

Study of the Symptoms’ Severity and Adherence to Therapy of Myelofibrosis Patients Treated With Ruxolitinib

Vera Stoeva, Guenka Petrova, Konstantin Mitov, Konstantin Tihomirov Tachkov

Subject: Medicine And Pharmacology, Hematology Keywords: myelofibrosis; ruxolitinib; severity of symptoms; adherence to therapy

Online: 22 May 2023 (08:52:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1475.v1

Analysis of Skull Anatomy for 3D Cephalometry – Normative Data Reveal Four Different Skull Types

Leon Ludwigs, Christian Pape, Helena Sophie Visse, Christoph Runte, Ulrich Meyer, Dieter Dirksen

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: 3D cephalometry; norm skull; skull anatomy; Procrustes analysis

Online: 22 May 2023 (08:41:02 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1471.v1

Complete Full Arch Supported by Short Implant (>8MM) in Edentulous Jaw: A Systematic Review

Alessio Rosa, Alberto Maria Pujia, Claudio Arcuri

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: full arch; mandibular atrophy; short dental implants; short implants; prosthetic

Online: 22 May 2023 (07:26:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1470.v1

Lessons from a 3-Year Review of PSMA PET-CT in a Tertiary Setting: Can We Fine Tune Referral Criteria by Identifying Factors Predicting Positivity and Negativity?

Vineet Pant, Sobhan Vinjamuri, Ahmad zaid Zanial, Faisal Naeem

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: PSMA PET CT; Biochemical Recurrence; prostate cancer recurrence; prostate bed recurrence

Online: 22 May 2023 (07:25:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1466.v1

Phenolic Composition and Wound Healing Potential Assessment of Moroccan Henna (Lawsonia inermis) Aqueous Extracts

Soukaina El Massoudi, Abdellah Zinedine, João Miguel Rocha, Meryem Benidir, Ilham Najjari, Lahsen El Ghadraoui, Meryem Benjelloun, Faouzi Errachidi

Subject: Medicine And Pharmacology, Pharmacy Keywords: Lawsonia inermis; Henna; leaves; phytochemical; wound healing activity; Morocco

Online: 22 May 2023 (05:30:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1464.v1

Influence of Dynamics of Changes in the Physical Properties of Osteoconductive Granulated Biopolymers on Scafold Stability in the Postoperative Period

Oleg V. Slesarev, Darya V. Malchikova, Vladimir I. Platonov, Natalia A. Maksimenko, Alexey A. Melnikov, Mariya A. Anikina, Ravshanbek B. Abdullaev

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: osteoconduction; osteoconductive granulated biopolymers; compaction coefficient; adsorption capacity; bone growth factors

Online: 22 May 2023 (05:09:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1459.v1

Regulation of Cathelicidin Antimicrobial Peptide (CAMP) Gene Expression by TLR9 in Adipocytes and in Adipose Tissue

Alexandra Höpfinger, Andreas Schmid, Leonie Schweitzer, Marissa Patz, Anja Weber, Andreas Schäffler, Thomas Karrasch

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: TLR (toll-like receptor) 9; cathelicidin anti-microbial peptide; CAMP; TNFα; adipocyte; adipose tissue; innate immunity

Online: 22 May 2023 (03:55:19 CEST)

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Understanding the complex interactions between metabolism and the immune system (“meta-flammation”) is crucial for the identification of key immunomodulatory factors as potential therapeutic targets in obesity and in cardiovascular diseases. Cathelicidin antimicrobial peptide (CAMP) is an important factor of innate immunity and is expressed in adipocytes. CAMP therefore might play a role as an adipokine in metaflammation and adipose inflammation. Toll-like receptor (TLR) 9 is another component of the innate immune system that is expressed and functionally active in adipocytes. The aim of the present study was to investigate the impact of TLR9 signaling on CAMP expression in adipocytes and in adipose tissue. CAMP gene expression in murine 3T3-L1 and human SGBS adipocytes and in murine and human adipose tissues was quantified by real-time PCR. TLR9 knockdown was applied in murine 3T3-L1 adipocytes via siRNA transfection. Adipocyte inflammation was induced in vitro by TNFα stimulation. Serum CAMP concentrations in TLR9 knockout (KO) and in wildtype mice were quantified by ELISA. CAMP gene expression was considerably increased in 3T3-L1 adipocytes during differentiation. TNFα significantly induced CAMP gene expression in mature adipocytes, which was antagonized by inhibitors of NF-κB and PI3K signaling. Cell-free nucleic acids (cfDNA) as endogenous TLR9 ligand significantly impaired CAMP gene expression, whereas synthetic agonistic and antagonistic TLR9 ligands had no effect. Cellular TLR9 knockdown reduced adipocyte CAMP gene expression in vitro and male TLR9 knockout mice exhibited lower systemic CAMP concentrations than wildtype mice. CAMP and TLR9 gene expression were correlated positively in murine and human subcutaneous but not in intraabdominal/visceral adipose tissues. These findings suggest a regulatory role of TRL9 in adipocytic CAMP expression as a novel putative molecular mechanism in adipose tissue innate immunity.

Preprint REVIEW | doi:10.20944/preprints202305.1454.v1

Relevance of Genetic Identification and Kinship Analysis in Human and Natural Catastrophes – A Review

Alejandra Real-Picado, Luis Diaz, Cláudia Gomes

Subject: Medicine And Pharmacology, Other Keywords: Catastrophes; Genetic identification; Kinship analysis; DNA degradation

Online: 22 May 2023 (03:01:25 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1444.v1

GABA Receptor Subtypes Involvement of Analgesia: Current Status

Xunjia Qian, Xinyi Zhao, Lulu Yu, Yujian Yin, Xiao-Dan Zhang, Liyun Wang, Jialie Luo, Jun-Xu Li, Qing Zhu

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Gamma-aminobutyric acid; subtype; analgesic effect; mechanism

Online: 19 May 2023 (15:03:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1435.v1

Perturbations of Gut Microbiota Compositions in Autism Spectrum Disorder: A Review and Meta-analysis

Tianxu Zhang, Yuan Zhou, Xuechao Zhang, Juan Wang

Subject: Medicine And Pharmacology, Anatomy And Physiology Keywords: Autism spectrum disorder; microbiota; meta-analysis

Online: 19 May 2023 (10:54:34 CEST)

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Medicine and Pharmacology