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Preprint REVIEW | doi:10.20944/preprints202309.0365.v1

Mammalian Models in Alzheimer's Research: An update

Himadri Sharma, Keun-A Chang, John Hulme, Seong Soo A An

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Animal model; Alzheimer’s Disease; Amyloid β; Tau Protein; LOAD models; Transgenic Murine Models

Online: 6 September 2023 (04:02:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.2193.v1

IL1beta—Biomarker for Ischemic Stroke Prognosis and Carotid Atheromatosis

Gabriela Catana, Ioan-Adrian Popentiu, Mădălina Văleanu, Corina Roman Filip, Romeo-Gabriel Mihăilă

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: inflammation; stroke; atheromatosis; biomarker; interleukin 1 beta

Online: 31 August 2023 (12:47:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1226.v1

Morphofunctional Investigation in a Transgenic Mouse Model of Alzheimer’s Disease: Non-Reactive Astrocytes Are Involved in Aβ Load and Reactive Astrocytes in Plaque Build-Up

Daniele Lana, Jacopo Junio Valerio Branca, Giovanni Delfino, Maria Grazia Giovannini, Fiorella Casamenti, Pamela Nardiello, Monica Bucciantini, Massimo Stefani, Petr Zach, Sandra Zecchi-Orlandini, Daniele Nosi

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: neuroinflammation; neurodegeneration; hippocampus; glial cells; cell-cell interactions; clasmatodendrosis; amyloid plaques; Aβ-aggregates; transgenic mouse; confocal microscopy

Online: 17 August 2023 (09:37:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1038.v1

Anomaly-Induced Quenching of g_A in Nuclear Matter and Impact on Search for Neutrinoless ββ Decay

Mannque Rho

Subject: Physical Sciences, Nuclear And High Energy Physics Keywords: fundamental quenching of gA; solution to quenched gA; neutrinoless double beta decay; Landau fixed point vs. ESPM; pseudo-conformality in nucleus

Online: 16 August 2023 (04:24:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0834.v1

Activation of Sarco/Endoplasmic Reticulum Ca2+-ATPase by Natural Polyphenolic Compounds. Mechanism of Protection against Oxidative Stress

Vladimír Heger, Benesova Barbora, Magdalena Majekova, Petronela Rezbarikova, Gabor Girst, Sara Hassan Hassan Ahmed, Tímea Gonda, Attila Hunyadi, Lubica Horakova

Subject: Biology And Life Sciences, Life Sciences Keywords: SERCA activity; insulin release; pancreatic beta-cells; polyphenolic compounds

Online: 10 August 2023 (08:21:09 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.0710.v1

Mitochondrial Dynamics and Insulin Secretion

Uma D. Kabra, Martin Jastroch

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: diabetes; mitochondrial dynamics; glucose-stimulated insulin secretion; pancreatic beta cell; fusion; fission

Online: 9 August 2023 (08:22:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0254.v1

Compact vs. Linear: Effects of Forest Structure, Patch Shape and Landscape Configuration on Black Alder Macromoth Communities

Sara La Cava, Margherita Lombardo, Vincenzo Bernardini, Federica Fumo, Giuseppe Rijllo, Rosario Turco, Laura Bevacqua, Giada Zucco, Stefano Scalercio

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Lepidoptera; landscape ecology; riparian forest; Alnus glutinosa; natural park; beta-diversity; South Italy

Online: 3 August 2023 (08:35:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0182.v1

Multipronged Approach to Profiling Metabolites in Beta Vulgaris L Dried Pulp Extracts Using Chromatography, NMR, and Other Spectroscopy Methods

Joshua Fiadorwu, Kiran Subedi, Daniel A Todd, Mufeed M Basti

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Beetroot (Beta vulgaris); Total Correlation Spectroscopy (TOCSY); Gas Chromatog-raphy-Mass Spectrometry (GC-MS); selective TOCSY; Homonuclear single quantum correlation (HSQC); fatty acid methyl esters (FAME)

Online: 2 August 2023 (10:01:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.2120.v1

Mixed Medicinal Mushroom Mycelia Attenuates Alzheimer's Disease Pathologies In Vitro and In Vivo

Ji Heun Jeong, Geum-Lan Hong, Young Gil Jeong, Nam Seob Lee, Do Kyung Kim, Jong Yea Park, Mina Park, Hyun Min Kim, Ya El Kim, Yung Choon Yoo, Seung Yun Han

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Alzheimer’s disease, Medicinal mushroom, Amyloid β, Zinc, 5XFAD

Online: 31 July 2023 (11:31:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.2054.v1

Genomic Characterization of IMP-Producing Pseudomonas aeruginosa in Bulgaria Reveals the Emergence of IMP-100, a Novel Plasmid-Mediated Variant Coexisting with a Chromosomal VIM-4

Ivan Stoikov, Ivan N. Ivanov, Deyan Donchev, Deana Teneva, Elina Dobreva, Rumyana Hristova, Stefana Sabtcheva

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Pseudomonas; metallo-beta-lactamase; blaIMP; novel allele; plasmid

Online: 31 July 2023 (04:53:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0878.v1

Electrochemical Investigations of Aloysia citrodora Paláu L. Water Extract for Iron-Chelation Abilities in Alzheimer’s Disease

Lee Baker, Ritu Kataky, Melanie-Jayne R. Howes, Paul L. Chazot

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Beta-amyloid; Aloysia Citrodora; Cyclic Voltammetry; Differential Pulse Voltammetry

Online: 13 July 2023 (04:52:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0732.v1

Repurposing Diltiazem for Its Neuroprotective Anti-dementia Role Against Intra-Cerebroventricular Streptozotocin-Induced Sporadic Alzheimer’s Disease-Type Rat Model

Ramesh Alluri, Spandana Rajendra Kopalli, Eswar Kumar Kilari, Praveen Kumar Pasala, Sushruta Koppula

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Alzheimer’s disease; diltiazem; intra-cerebroventricular; streptozotocin; cognition; amyloid beta; anti-oxidant enzymes

Online: 11 July 2023 (12:29:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1853.v1

Breaking Barriers in Lung Cancer Research: The Intriguing Connection between IL-1β and PD-1/PD-L1 Pathways

Dan Ran Castillo, Won Jin Jeon, Daniel Park, Bryan T. Pham, Chieh Yang, Bowon Joung, Jin Hyun Moon, Jae Lee, Esther G. Chong, Kiwon Park, Mark E. Reeves, Penelope Duerksen-Hughes, Hamid Mirshahidi, Saied Mirshahidi

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: interleukin-1 beta (IL-1β); immune-checkpoint inhibitors (ICIs); non-small cell lung cancer (NSCLC); programmed death ligand 1 (PD-L1); therapeutic resistance

Online: 27 June 2023 (07:16:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1178.v1

Implementing an On-Slide Molecular Classification of Gastric Cancer: A Tissue Microarray Study

Simona Costache, Rebecca De Havilland, Sofia Diaz McLynn, Maria Sajin, Sarah Wedden, Corrado D'Arrigo

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: gastric cancer; molecular classification; EBER; MMR; E-cadherin; beta-catenin; p53; Her2; PD-L1; Claudin18.2

Online: 16 June 2023 (05:46:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0903.v1

Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera

Yit Ng, Seok Shin Tan, Foong Ming Moy

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Rapid; beta-carotene; UHPLC; human sera; Malaysia

Online: 13 June 2023 (08:41:31 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0629.v1

Metabolic Reprogramming Toward Aerobic Glycolysis Involved in the Brain Amyloid Pathology

Toshiyuki Murai, Satoru Matsuda

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: glucose metabolism; aerobic glycolysis; Warburg effect; Alzheimer’s disease; amyloid-β; ketogenic diet

Online: 8 June 2023 (10:39:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0366.v1

ART FounDATion-LOG (ARabidopsis Transcription regulatory Factor Domain-domain interaction Analysis Tool-Liquid liquid phase separation, Oligomerization, Go analysis), a toolkit for interaction data based domain analysis

Jee Eun Kang, Ji Hae Jun, Jung Hyun Kwon, Ju-Hyun Lee, Kidong Hwang, Sungjong Kim, Namhee Jeong

Subject: Biology And Life Sciences, Plant Sciences Keywords: Liquid liquid phase separation; protein oligomerization; GO; domain-domain interaction; domain linker; intrinsically disordered regions; domain-peptide interaction; beta-sheet; transmembrane helices; post-translational modification

Online: 6 June 2023 (03:43:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0091.v1

Functionalized Collagen/Poly(Ethylene Glycol) Diacrylate Interpenetrating Network Hydrogel Enhances Beta-Pancreatic Cell Sustenance

Natalia Moreno-Castellanos, Elías Cuartas-Gomez, Oscar Vargas-Ceballos

Subject: Chemistry And Materials Science, Biomaterials Keywords: Collagen; hydrogel; interpenetrating network; beta pancreatic cells; vascular endothelial factor; biomaterial

Online: 1 June 2023 (12:48:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0051.v1

Combination of Metal Nanoparticles With Hydrolytic Enzymes To Obtain Improved Antifungals

Ilya Lyagin, Aysel Aslanli, Maxim Domnin, Nikolay Stepanov, Olga Senko, Olga Maslova, Elena Efremenko

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: green synthesis; MOFs; amyloid proteins; prionase; mycotoxins; growth inhibition; quorum sensing; quorum quenching; lactamases; biofilms; chitinases; lactonases; His6-OPH

Online: 1 June 2023 (07:31:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1698.v1

Comparison of Deeply Versus Mildly Invasive Mesothelioma Proteomes Identifies Potential Links Between Mitochondrial Acadvl Expression and Biomarkers of Aggressiveness

Daniel L. Pouliquen, Joanna Kopecka, Stéphanie Blandin, Alice Boissard, Cécile Henry, Chiara Riganti, Catherine Guette

Subject: Biology And Life Sciences, Life Sciences Keywords: Cancer; Aggressiveness; Mitochondria; Acadvl; Fatty acid beta oxidation; Biomarker; Proteomics; Malignant mesothelioma

Online: 24 May 2023 (05:06:41 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1547.v1

Realization of Amyloid like Aggregation as a Common Cause for the Pathogenesis in Diseases

Soumick Naskar, Nidhi Gour

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Amyloid; Aggregation; Self-assembly; Gene mutation; Disease Pathogenesis; Inborn errors of metabolism

Online: 23 May 2023 (03:35:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1347.v1

Amyloid ß_1–42 Peptide Induces Galectin-1^S8 O-GlcNAcylation to Lead Microglia Migration

Alazne Arrazola Sastre, Miriam Luque Montoro, Francisco LLavero, José L Zugaza

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Amyloid ß1-42 peptide; OGT; O-GlcNAcylation; Galectin-1, Galectin-1Serine 8- O-GlcNAcylation; Gal-1S8A; microglia; migration

Online: 18 May 2023 (12:52:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0781.v1

Protective Role of an Extract Waste Product From Citrus bergamia in an In Vitro Model of Neurodegeneration

Jessica Maiuolo, Francesca Bosco, Lorenza Guarnieri, Saverio Nucera, Stefano Ruga, Francesca Oppedisano, Luigi Tucci, Carolina Muscoli, Ernesto Palma, Angelo Maria Giuffrè, Vincenzo Mollace

Subject: Public Health And Healthcare, Other Keywords: Citrus bergamia; Fiber of bergamot; by-products; pastazzo; β-amyloid protein; Alzheimer’s disease; Neurons; Oligodendrocytes

Online: 11 May 2023 (04:24:32 CEST)

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A balanced diet model, rich in fruits and vegetables and ensuring the intake of natural products, has been shown to reduce or prevent the occurrence of many chronic diseases. However, the choice to consume large quantities of fruits and vegetables leads to an increase in the amount of waste, which can cause the alteration in environmental sustainability. To date, the concept of "by-product" has evolved, understood as a waste product, from which it is still possible obtain useful compounds. Therefore, the by-products in the agricultural sector, are a rich source of bioactive compounds, capable to possess a second life, decreasing the amount of waste products, the disposal costs and the environmental pollution. A promising and well-known citrus of the Mediterranean diet is the bergamot (Citrus bergamia, Risso et Poiteau). The composition of bergamot is known and the rich presence of phenolic compounds and essential oils has justified countless beneficial properties found, including anti-inflammatory, antioxidant, anti-cholesterolemic and protective activity for the immune system, heart failure and coronary heart diseases. The industrial processing of bergamot fruits leads to the formation of bergamot juice and bergamot oil. The solid residues, referred as "pastazzo", are normally used as feed for livestock or pectin production. The fiber of bergamot (BF) can be obtained from pastazzo and could exert an interesting effect thanks to its content of polyphenols. The purpose of this work was to test the effects of BF on an in vitro model of neurotoxicity induced by treatment with amyloid beta protein. In particular, this experimental model included both neurons and oligodendrocytes in order to measure the involvement of the glia and compare it with the neurons one. The results obtained showed a protective activity of BF, although the oligodendrocytes were more sensitive and fragile than neurons. Further experiments are necessary and if the trend was confirmed, solid residues of bergamot could be used in AD, and, at the same time, could help to avoid the accumulation of waste products.

Preprint ARTICLE | doi:10.20944/preprints202305.0322.v1

Consecutive Four-Component Coupling-Addition-Aza-Anellation-Pictet-Spengler Synthesis of Tetrahydro-Beta-Carbolines – Optimized Michael Addition and Computational Study on the Aza-Anellation Step

Kai Ries, Françoise A. Aouane, Thomas J. J. Müller

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: aza-Michael addition; tetrahydro-beta-carbolines; catalysis; one-pot reaction; multicomponent reaction; ytterbium(III) triflate

Online: 5 May 2023 (07:45:16 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202305.0275.v1

Chelator PBT2 Forms a Ternary Cu²⁺ Complex with β-Amyloid that has High Stability but Low Specificity

Simon Drew

Subject: Biology And Life Sciences, Biophysics Keywords: 8-hydroxyquinoline; PBT2; amyloid; copper; terdentate; ternary; antimicrobial

Online: 5 May 2023 (02:36:19 CEST)

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The metal chelator PBT2 (5,7-dichloro-2-[(dimethylamino)methyl]-8-hydroxyquinoline) acts as a terdentate ligand capable of forming binary and ternary Cu2+ complexes. It was clinically trialed as an Alzheimer’s disease (AD) therapeutic but failed to progress beyond phase II. The β-amyloid (Aβ) peptide associated with AD was recently concluded to form a unique Cu(Aβ) complex that is inaccessible to PBT2. Herein, it is shown that the species ascribed to this binary Cu(Aβ) complex in fact corresponds to ternary Cu(PBT2)NIm[Aβ] complexes formed by anchoring of Cu(PBT2) on imine nitrogen (NIm) donors of His side chains. The primary site of ternary complex formation is His6, having a conditional stepwise formation constant at pH 7.4 (K [M−1] ) of log K = 6.4 ± 0.1, and a second site is supplied by His13 or His14 (log K = 4.4 ± 0.1). The stability of Cu(PBT2)NIm[H13/14] is comparable with that of the simplest ternary complexes involving free imidazole (log K = 4.22 ± 0.09) and histamine (log K = 4.00 ± 0.05). The 100-fold larger formation constant for Cu(PBT2)NIm[H6] indicates that outer-sphere ligand–peptide interactions strongly stabilize its structure. Despite the relatively high stability of Cu(PBT2)NImH6, PBT2 is a promiscuous Cu2+-binding ligand capable of forming a ternary Cu(PBT2)NIm complex with any ligand containing NIm donor. These ligands include histamine, L-His, and ubiquitous His side chains of peptides and proteins in the extracellular milieu, whose combined effect should outweigh that of a single Cu(PBT2)NIm[H6] complex regardless of its stability. We therefore conclude that PBT2 is capable of accessing Cu(Aβ) complexes with high stability but not specificity. The results have implications for future AD therapeutic strategies and understanding the role of PBT2 in the bulk transport of transition metal ions. Given the repurposing of PBT2 as a drug for breaking antibiotic resistance, ternary Cu(PBT2)NIm and analogous Zn(PBT2)NIm complexes may be relevant to its antimicrobial properties.

Preprint ARTICLE | doi:10.20944/preprints202304.1265.v1

Nucleolar Stress-Induced Membrane-Less Organelles: Proteome Analysis through the Prism of Liquid-Liquid Phase Separation

Yakov I. Mokin, Anastasia A. Gavrilova, Anna S. Fefilova, Irina M. Kuznetsova, Konstantin K. Turoverov, Vladimir N. Uversky, Alexander V. Fonin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloid bodies; intrinsically disordered protein; liquid-liquid phase separation; membrane-less organelle; nuclear stress bodies; nucleolar biomolecular condensates

Online: 30 April 2023 (05:18:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0899.v1

Ordinary Muon Capture on ¹³⁶Ba: Comparative Study Using the Shell Model and pnQRPA

Patricia Gimeno, Lotta Jokiniemi, Jenni Kotila, Marlom Ramalho, Jouni Suhonen

Subject: Physical Sciences, Nuclear And High Energy Physics Keywords: neutrino physics; muon-capture rates; double beta decays; weak interaction

Online: 25 April 2023 (08:01:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0844.v1

Extended Exton’s Triple and Horn’s Double Hypergeometric Functions and Associated Bounding Inequalities

Rakesh K. Parmar, Junesang Choi, Saravanan S.

Subject: Computer Science And Mathematics, Mathematics Keywords: Extended Beta function; Extended hypergeometric function; Extended confluent hypergeometric function; Extended Appell function; Mellin transforms; Inverse Mellin transforms; H-functions; Laguerre polynomials; Transformation formulas; Recurrence relation; Generating function; Bounding inequalities

Online: 24 April 2023 (09:45:32 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202303.0476.v2

An Alternative View of Familial Alzheimer’s Disease Genetics

Michael Lardelli

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Alzheimer's Disease; Familial Alzheimer Disease (FAD); presenilins; Amyloid beta Precursor Protein; mutation

Online: 23 April 2023 (02:50:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0308.v1

Effects of Different Feeding Patterns on the Rumen Bacterial Community of Tan Lambs, based on High-Throughput Sequencing of 16S rDNA Amplicons

Lili Zhang, Yanliang Bi, Jie Zhang, Zilin Fu, Xiaofeng Xu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Alpha diversity; Beta diversity; Mutton quality; Pasture-fed; Pen-fed; Tan lambs

Online: 13 April 2023 (08:01:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202303.0533.v1

Biomarkers of neurodegeneration in post-traumatic stress disorder: an integrative review

Ravi Philip Rajkumar

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: post-traumatic stress disorder; dementia; Parkinson’s disease; β-amyloid; inflammation; hippocampus; white matter integrity; genetics

Online: 31 March 2023 (02:12:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202301.0569.v1

A Novel Animal Model in Diabetes Research: Regeneration of β cells in the Axolotl Salamander?

Pernille Lajer Sørensen, Anita Dittrich, Henrik Lauridsen

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: diabetes; type 1 diabetes; regeneration; axolotl; salamander; beta cell; beta cell regeneration; streptozotocin

Online: 31 January 2023 (04:11:24 CET)

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Diabetes is a group of diseases characterized by loss of β cell mass and/or -function, resulting in hyperglycemia. Approx. 537 million people worldwide suffer from diabetes – a number which is expected to increase. Diabetes is primarily treated by exogenous insulin, which comes with the challenges of maintaining glycemic control to prevent ketoacidosis and severe complications. The need for a curative treatment has initiated the research in β cell regeneration. Several studies in mice have identified essential genes for β cell fate, which can be manipulated in other cells to induce generation of new β cells. Zebrafish, a regeneration-positive animal model, has shown several different sources of new β cells, including regeneration by self-replication, neogenesis by duct-associated progenitor cells, and transdifferentiation of other endocrine islet cells. The animal models used in this research area are either limited by their low regenerative ability (mice), or their small size and remoteness from humans (zebrafish). There is a need for new animal models of diabetes, in which the molecular pathways of endogenous regeneration can be studied. This study proposes the axolotl salamander (Ambystoma mexicanum) as a model for studying the regeneration of β cells. The axolotl has shown great regenerative capability, as they have proven capable of regenerating amputated limbs, and hearts with myocardial infarction, among other organs. This study aims to establish a diabetic axolotl model, investigate their regenerative ability in the pancreas, and examine the potential systemic effects of the induced disease. In a pilot study, five different protocols using STZ (streptozotocin) were tested, and the most optimal protocol was found. Furthermore, the glucose tolerance test was optimized to characterize the glycemic state of the animals. The effect of the treatment on blood glucose levels was measured to characterize the development and decline of the disease. The histological changes in the pancreas were examined. Moreover, the systemic effects of the STZ treatment were investigated in blood and urine. The study indicated that it is possible to induce diabetes in the axolotl, but variations between the animals should be minimized, or the sample size should be increased to conduct a satisfying experiment, as it was not possible to induce diabetes in all animals. Regeneration was not observed histologically, but a restoration of blood glucose levels was seen over the span of the experiment. Lastly, edema formation was observed in some of the STZ-treated animals, but the cause of edema remains undetermined.

Preprint ARTICLE | doi:10.20944/preprints202212.0382.v2

Anti-Inflammatory Effects, Protection of Gut Barrier Integrity and Stimulation of Phagocytosis of postbiotic combination ABB C1^®

Maria Tintoré, Jordi Cuñé, Vaclav Vetvicka, Carlos De Lecea

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Saccharomyces cerevisiae; beta-glucans; selenium; zinc; gut barrier; modulation; COVID-19; nutritional supplementation; allergy; inflammatory process

Online: 25 January 2023 (04:43:01 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0397.v2

The Influence of Skin Commensals on the Therapeutic Outcomes of Surgically-Debrided Diabetic Foot Infections – A Large Retro-Spective Comparative Study

Ilker Uçkay, Dan Lebowitz, Benjamin Kressmann, Benjamin Alan Lipsky, Karim Gariani

Subject: Medicine And Pharmacology, Surgery Keywords: antibiotic therapy; diabetic foot infections; non-beta-lactam antibiotics; skin commensals; treatemet failures; associations with treatment failures

Online: 20 January 2023 (15:13:51 CET)

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Preprint REVIEW | doi:10.20944/preprints202301.0239.v1

The Nerve Growth Factor Receptor (NGFR/P75^ntr): A Major Player in Alzheimer’s Disease

Francesco Bruno, Paolo Abondio, Alberto Montesanto, Donata Luiselli, Amalia Cecilia Bruni, Raffaele Maletta

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Alzheimer’s disease; Nerve growth factor receptor; NGFR; p75NTR; amyloid-beta; expression; signaling pathways; neuropathology; diagnosis; treatment.

Online: 13 January 2023 (07:37:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0462.v1

Structure of a Metacommunity of Urban Bees: Species Diversity and Spatio-temporal Modularity

Yoko L. Dupont, Henning Bang Madsen, Mette Balslev Greve, Claus Rasmussen, Jens M. Olesen

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Apis; beta diversity; city; nestedness; network; pollinator

Online: 26 December 2022 (02:50:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0342.v1

TGF-beta Modulates the Integrity of the Blood Brain Barrier in Vitro, and is Associated with Metabolic Alterations in Pericytes

Leonie Schumacher, Redouane Slimani, Laimdota Zizmare, Jakob Ehlers, Felix Kleine Borgmann, Julia C. Fitzgerald, Petra Fallier-Becker, Anja Beckmann, Alexander Grißmer, Carola Meier, Ali El-Ayoubi, Kavi Devraj, Michel Mittelbronn, Christoph Trautwein, Ulrike Naumann

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Glioblastoma; blood brain barrier; transforming growth factor beta; NMR metabolomics

Online: 20 December 2022 (01:19:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0072.v1

A New Statistical Model Based on the Novel Generalized Odd Beta Prime Family of Continuous Probability Distributions with Applications to Cancer Disease Data Sets

Ahmad Abubakar Suleiman, Mahmod Othman, Aliyu Ismail Ishaq, Mohd Lazim Abdullah, Rachmah Indawati, Hanita Daud, Rajalingam Sokkalingam

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: beta prime distribution; Weibull distribution; mixture representations; information generating function; entropies; order statistics; Monte Carlo simulation; blood cancer disease

Online: 5 December 2022 (09:47:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0135.v1

Rare Amyloid Precursor Protein Point Mutations Recapitulate Worldwide Migration and Admixture in Healthy Individuals: Implications For the Study of Neurodegeneration

Paolo Abondio, Francesco Bruno, Amalia Cecilia Bruni, Donata Luiselli

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloid; Alzheimer’s disease; neurodegenerative diseases; neurodegeneration; dementia; population genomics; migration; admixture; APP; neuropathology

Online: 8 November 2022 (02:10:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202210.0167.v1

Trivariate Joint Distribution Modelling of Compound Events Using the Nonparametric D-Vine Copula Developed Based on Bernstein and Beta Kernel Copula Density Framework

Shahid Latif, Slobodan P. Simonovic

Subject: Engineering, Civil Engineering Keywords: compound flooding; D-vine copula; trivariate joint analysis; Bernstein estimator; Beta kernel estimator; parametric copulas; kernel density estimation; return periods

Online: 12 October 2022 (09:04:04 CEST)

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Low-lying coastal communities are often threatened by compound flooding (CF), which can be determined through the joint occurrence of storm surges, rainfall and river discharge either successively or in close succession. The trivariate distribution can demonstrate the risk of the compound phenomenon more realistically rather than considering each contributing factor independently or in a pairwise dependency. Recently vine copula has been recognized as the highly flexible approach to constructing a higher dimensional joint density framework. In such construction, parametric class copula with parametric univariate marginal distributions is often involved. Such incorporation can lack flexibility due to parametric functions with prior distribution assumptions about their univariate marginal and/or copula joint density. This study introduces the vine copula approach in a nonparametric setting by introducing Bernstein and Beta kernel copula density in establishing trivariate flood dependence. The proposed model is applied to 46 years of flood characteristics collected on the west coast of Canada. The univariate flood marginal distribution is modelled using nonparametric kernel density estimation (KDE). The 2-D Bernstein estimator and Beta kernel copulas estimator are tested independently in capturing pairwise dependencies to establish D-vine structure in a stage-wise nesting approach in three alternative ways, each by permutating the location of the conditioning variable. The best-fitted vine structure is selected using goodness-of-fit (GOF) test statistics. The performance of the nonparametric vine approach is also compared with the vine constructed in the parametric and semiparametric fitting procedure. Investigation reveals that the D-vine constructed using Bernstein copula with normal KDE marginals nonparametrically performed well in capturing dependence of the compound events. Finally, the derived nonparametric model is used in the estimation of trivariate OR- and AND-joint return periods, further employed in estimating failure probability (FP) statistics. The trivariate return periods for the AND-joint case are higher than for the OR-joint case for the same flood combination. Also, the trivariate flood hazard results in a high-value FP than bivariate and univariate events. Ignoring the trivariate dependence could result in the underestimation of FP

Preprint REVIEW | doi:10.20944/preprints202210.0056.v1

Structural Determinant of β-Amyloid Formation: From Transmembrane Protein Dimerization to β-Amyloid Aggregates

Nicolas Papadopoulos, Nuria Suelves, Florian Perrin, Devkee M. Vadukul, Céline Vrancx, Stefan N. Constantinescu, Pascal Kienlen-Campard

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Alzheimer’s disease; Amyloid Precursor Protein; Amyloid beta; APP-C99; dimerization; Orientations; Aggregation; Oligomerization

Online: 6 October 2022 (08:41:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0551.v1

Methylprednisolone Induced Lymphocytosis: A Prospective Study in 20 Ppatients with Immune Mediated Inflammatory Disorders

Camille Beniada, Bruno Couturier, Florence Reye, Cedric Delporte, Pierre Van Antwerpen, Viviane De Maertelaer, Elie Cogan

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Glucocorticoids; lymphocytosis; methylprednisolone; beta-blockers; adrenergic tone; IMID

Online: 31 August 2022 (16:25:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0184.v1

Amyloid Precursor Protein, Alpha-, Beta- and Gamma-Secretases Expression in Penumbra Cells after Photothrombotic Stroke. Evaluation of the Neuroprotective Effect of Secretase Inhibitors

Svetlana Sharifulina, Valeria Guzenko, Alexandr Logvinov, Andrey Khaitin, Yuliya Kalyuzhnaya, Natalia Dobaeva, Yan Li, Lei Chen, Вin He, Svetlana Demyanenko

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloid precursor protein; photothrombotic stroke; ischemia; alpha-secretase; beta-secretase; gamma-secretase

Online: 13 May 2022 (08:39:09 CEST)

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Preprint REVIEW | doi:10.20944/preprints202205.0166.v1

Antimicrobial Peptides (AMPs) In the Pathogenesis of Alzheimer’s Disease: Implications for Diagnosis and Treatment

Francesco Bruno, Antonio Malvaso, Sonia Canterini, Amalia Cecilia Bruni

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Antimicrobial Peptides (AMPs); Alzheimer’s Disease (AD); infectious hypothesis; beta-amyloid (Ab); lactoferrin; defensins; cystatins; thymosin β4; histatin 1; statherin

Online: 12 May 2022 (09:40:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0030.v1

ZIKA Virus Envelope Protein Forms Disulfide Bond-Dependent Polymers: Evidence for A Redox Imbalance during Infection

Grégorie Lebeau, Jonathan Turpin, Etienne Frumence, Daed El Safadi, Wissal harrabi, Philippe Desprès, Pascale Krejbich-Trotot, wildriss viranaïcken

Subject: Biology And Life Sciences, Virology Keywords: Zika virus; unfolded protein response; ER stress; oligomer; disulfide bond amyloid aggregates

Online: 6 April 2022 (05:24:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0246.v1

Local and Global Protein Interactions Contribute to Residue Entrenchment in Beta-Lactamase TEM-1

André Birgy, Mélanie Magnan, Claire Amaris Hobson, Matteo Figliuzzi, Karine Panigoni, Cyrielle Codde, Olivier Tenaillon, Hervé Jacquier

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: entrenchment; protein stability; TEM-1 beta-lactamase; CTX-M-15 beta-lactamase; M182T mutation

Online: 17 March 2022 (08:43:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202203.0133.v1

A New Technique to Repair Large Bone Defects

Takaaki Tanaka, Hirokazu Komaki, Naoya Inagaki, Shoshi Akiyama, Mitsuru Saito

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: bone formation; BMP-2; FGF-2; beta-TCP; osteoblast-osteoclast communication

Online: 9 March 2022 (10:58:33 CET)

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Preprint HYPOTHESIS | doi:10.20944/preprints202109.0437.v2

The Glial Aging & Senescence Hypothesis of Late-onset Alzheimer’s

Victor Lau, Leanne Ramer, Marie-Ève Tremblay

Subject: Biology And Life Sciences, Aging Keywords: Alzheimer's; Cellular senescence; Neurodegeneration; Microglia; Astrocytes; Oligodendrocyte Progenitor Cells; TREM2; Braak staging; Oxidative stress; Phapoptosis; Amyloid-beta; Tau; Neuritic plaques

Online: 7 March 2022 (14:22:25 CET)

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Alzheimer’s disease (AD) predominantly occurs as a late-onset form (LOAD), involving neurodegeneration and cognitive decline with progressive memory loss. Over time, risk factors and aging promote accumulation of well-known AD pathologies in oxidative stress, amyloid-beta and tau protein pathology, as well as inflammation. Homeostatic glial functions regulate and suppress these AD pathologies; however, other glial states involve increased pro-inflammatory cytokine release and further pathology accumulation. Different stresses can additionally induce cellular senescence, or an irreversible differentiation process resulting in decreased supportive functions and increased, pro-inflammatory cytokine release. While these pathophysiological underpinnings all contribute to LOAD, they require temporal and mechanistic integration. This perspective hypothesizes that when individuals have threshold senescent glia accumulation, they transition from healthy cognition into mild cognitive impairment and LOAD diagnosis. Particularly, senescent microglia are predicted to represent a final threshold required for the tau pathology burden and spreading that corresponds to sustained neurodegeneration and dementia severity. We first explore age-related decline in glia that promote increases in AD pathologies, and then discuss emerging evidence linking oxidative stress, neurons containing tau pathology, and amyloid-beta to microglia, oligodendrocyte progenitor cell, and astrocyte senescence. Our hypothesis proposes that senescent astrocytes and oligodendrocyte progenitors pressure microglia to phagocytose neurons containing tau pathology. The resulting senescent microglia would form neuritic plaques and induce paracrine senescence transitioning into a progressive clinical dementia presentation. This predictive hypothesis can potentially account for why medications used to treat LOAD fail, as previous treatments have not reduced senescent glial burden. It is also coherent with the predominant hypotheses surrounding LOAD, generates testable hypotheses about LOAD, and increases rationale in testing senolytics as targeted treatments for LOAD arrest and reversal.

Preprint ARTICLE | doi:10.20944/preprints202202.0012.v1

Experimental and Theoretical Studies on the Anti-Amyloidogenic and Destabilizing Effects of Pyrogallol Against Human Insulin Protein

Parastoo Shouhani, Seifollah Bahramikia, Seyed Hesam Hejazi

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Human Insulin, Amyloid Fibrils; Protein aggregation; Amorphous aggregates; Aromatic Compounds; Polyphenol; Pyrogallol

Online: 1 February 2022 (12:46:35 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0110.v2

Cocoa Extract Exerts Sex-Specific Effects in an Aggressive Hyper-Glycemia Model: A Pilot Study

Kathryn C. Racine, Lisard Iglesias-Carres, Jacob A. Herring, Mario G. Ferruzzi, Colin D. Kay, Jeffery S. Tessem, Andrew P. Neilson

Subject: Biology And Life Sciences, Food Science And Technology Keywords: BTBR; ob/ob; type-2 diabetes; flavanol; insulin; beta cell

Online: 31 January 2022 (18:09:34 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0156.v1

Comprehensive analysis of imipenemase (IMP)-type metallo-Beta-lactamase showing global distribution threating Asia

Pisut Pongchaikul, Paninee Mongkolsuk

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: beta-lactamase; carbapenemase; antimicrobial resistance

Online: 12 January 2022 (00:10:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0187.v1

The Compound Inverse Rayleigh as an Extreme Wind Speed Distribution and its Bayes Estimation

Elio Chiodo, Maurizio Fantauzzi, Giovanni Mazzanti

Subject: Engineering, Electrical And Electronic Engineering Keywords: renewable energy; bayes estimation; beta distribution; lognormal distribution; compound inverse Rayleigh distribution; extreme values; safety; wind power

Online: 10 December 2021 (14:03:52 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0108.v1

Fragment-Based Virtual Screening to Discover Potential Plasmodium PI4KIIIβ Ligands

Akachukwu Ibezim, Mbanefo S. Madukaife, Sochi C Osigwe, Nadja Engel, Ramanathan Karuppasamy, Fidele Ntie-Kang

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: Plasmodium; malaria; type III beta phosphatidylinositol 4-kinase; virtual screening; homology modeling; molecular dynamics

Online: 5 November 2021 (08:44:31 CET)

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Preprint REVIEW | doi:10.20944/preprints202110.0231.v1

Magnetic Resonance Imaging in Animal Models of Alzheimer’s Disease Amyloidosis

Ruiqing Ni

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Alzheimer’s disease; magnetic resonance imaging; animal model; amyloid-beta

Online: 15 October 2021 (15:06:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202110.0222.v1

Positron Emission Tomography in Animal Models of Alzheimer’s Disease Amyloidosis

Ruiqing Ni

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Alzheimer's disease; amyloid-beta; animal model; astrocyte; blood-brain barrier; imaging; metabolism; microglia; neuroinflammation, neurotransmitter receptors; positron emission tomography; synaptic density

Online: 15 October 2021 (11:02:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202110.0197.v1

Two Opposing Functions of Angiotensin-Converting Enzyme (ACE) That Links Hypertension, Dementia, and Aging

Duc Le, Lindsay Brown, Kundan Malik, Shin Murakami

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: AGING; Age-related comorbidities; Angiotensin-Converting Enzyme; Amyloid-degrading enzyme; Alzheimer’s disease; Dementia; Hypertension; Life extension; Stress resistance

Online: 13 October 2021 (11:04:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202110.0137.v1

Direct Interaction of ATP7B and LC3B Proteins Suggests a Cooperative Role of Copper Transportation and Autophagy

Supansa Pantoom, Adam Pomorski, Katharina Huth, Christina Hund, Janine Petters, Artur Krezel, Andreas Hermann, Jan Lukas

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Wilson disease; ATPase copper transporting beta; autophagosome-lysosome fusion; HepG2; LC3 interaction region

Online: 8 October 2021 (11:06:51 CEST)

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Preprint REVIEW | doi:10.20944/preprints202110.0005.v1

Human Brown Adipose Tissue and Metabolic Health: Potential for Therapeutic Avenues

Rajan Singh, Albert Barrios, Golnaz Dirakvand, Shehla Pervin

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: Brown adipose tissue; beige adipose tissue; obesity; cold exposure; uncoupling protein-1, beta-adrenergic receptor; energy expenditure; adipose browning; micro-RNA

Online: 1 October 2021 (11:30:39 CEST)

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Obesity-associated metabolic abnormalities comprise of a cluster of conditions including dyslipidemia, insulin resistance, diabetes, and cardiovascular diseases that has affected more than 650 million people all over the globe. Obesity results from accumulation of white adipose tissues mainly due to the chronic imbalance of energy intake and energy expenditure. Variety of approaches to treat or prevent obesity, including lifestyle interventions, surgical weight loss procedures and pharmacological approaches to reduce energy intake and increase energy expenditure have failed to substantially decrease the prevalence of obesity. Brown adipose tissue (BAT), the primary source of thermogenesis in infants and small mammals may represent a promising therapeutic target to treat obesity by promoting energy expenditure through non-shivering thermogenesis mediated by mitochondrial uncoupling protein 1 (UCP1). Since the confirmation of functional BAT in adult humans by several groups, approximately a decade ago and its association with a favorable metabolic phenotype, intense interest on the significance of BAT in adult human physiology and metabolic health has emerged within the scientific community to explore its therapeutic potential for the treatment of obesity and metabolic diseases. Substantially decreased BAT activity in individuals with obesity indicates a role for BAT in setting of human obesity. On the other hand, BAT mass and its prevalence has been reported to correlate with lower body mass index (BMI), decreased age and glucose levels, leading to lower incidence of cardio metabolic diseases. Increased cold exposure in adult humans with undetectable BAT was associated with decreased body fat mass and increased insulin sensitivity. Deeper understanding of the role of BAT in human metabolic health and its inter-relationship with body fat distribution and deciphering proper strategies to increase energy expenditure by either increasing functional BAT mass, or inducing white adipose browning holds the promise for possible therapeutic avenues for the treatment of obesity and associated metabolic disorders.

Preprint ARTICLE | doi:10.20944/preprints202109.0320.v1

A Novel Method to Stimulate Lymphatic Clearance of Beta-Amyloid from Mouse Brain Using Non-invasive Music-Induced Opening of the Blood-Brain Barrier with EEG Markers

Oxana Semyachkina-Glushkovskaya, Alexander Khorovodov, Ivan Fedosov, Alexey Pavlov, Alexander Shirokov, Ali Esmat Sharif, Alexander Dubrovskii, Inna Blokhina, Andrey Terskov, Nikita Navolokin, Arina Evsukova, Georgy Karandin, Dariya Elovenko, Maria Tzoy, Vasily Ageev, Ilana Agranovich, Valeria Telnova, Anna Tsven, Elena Saranceva, Tatyana Iskra, Jürgen Kurths

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: music; blood-brain barrier; lymphatic system; amyloid-β protein; detrended fluctuation analysis; electroencephalographic patterns.

Online: 20 September 2021 (09:02:40 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202109.0133.v1

Fluid and Tissue Biomarkers of Lewy Body Dementia: Report of an LBDA Symposium

Gregory D. Scott, Moriah R. Arnold, Thomas G. Beach, Christopher H. Gibbons, Anumantha G. Kanthasamy, Russell M. Lebovitz, Afina W. Lemstra, Leslie M. Shaw, Charlotte E. Teunissen, Henrik Zetterberg, Angela S. Taylor, Todd C. Graham, Bradley F. Boeve, Stephen N. Gomperts, Neill R. Graff-Radford, Charbel E.-H. Moussa, Kathleen L. Poston, Liana S. Rosenthal, Marwan N. Sabbagh, Ryan R. Walsh, Miriam T. Weber, Melissa J. Armstrong, Jee A. Bang, Andrea C. Bozoki, Kimiko Domoto-Reilly, John E. Duda, Jori E. Fleisher, Douglas R. Galasko, James E. Galvin, Jennifer G. Goldman, Samantha K. Holden, Lawrence S. Honig, Daniel E. Huddleston, James B. Leverenz, Irene Litvan, Carol A. Manning, Karen S. Marder, Alexander Y. Pantelyat, Victoria S. Pelak, Douglas W. Scharre, Sharon J. Sha, Holly A. Shill, Mari Zoltan, Joseph F. Quinn, David J. Irwin

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: cerebrospinal fluid; alpha-synuclein; skin biopsy; seeded aggregation assays; tau, amyloid; Lewy body dementia

Online: 7 September 2021 (16:26:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0092.v1

A Bridge Between the Breath and the Brain: Synchronization of Respiration, a Pupillometric Marker of the Locus Coeruleus, and an EEG Marker of Attentional Control State

Michael Christopher Melnychuk, Ian H. Robertson, Emanuele R.G. Plini, Paul M. Dockree

Subject: Social Sciences, Behavior Sciences Keywords: breath; respiration; synchronization; coupling,; EEG; theta-beta ratio; pranayama; meditation; attention; citta vritti

Online: 6 September 2021 (12:49:41 CEST)

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Preprint REVIEW | doi:10.20944/preprints202109.0073.v1

Mitochondrial Dysfunction: a Notable Contributor to the Progression of Alzheimer’s & Parkinson’s Disease

Abolaji Samson Olagunju, Abiola Adeyanju Alagbe, Titilayomi Ayomide Otenaike, Babatunde Oladayo Fabiyi, John Oluwafemi Teibo, Naeem Qusty, Safaa Qusti, Eida Mohammed Alshammari, Gaber El-Saber Batiha, Olabode Omotoso

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Mitochondrial dysfunction; Alzheimer's disease; Parkinson's disease; Neurodegeneration; Amyloid beta; Parkin

Online: 3 September 2021 (16:01:26 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202108.0115.v1

Intracellular Ion Changes Induced by the Exposure to Beta-Amyloid Can Be Explained by the Formation of Channels in the Lysosomal Membranes

Dmitry V Zaretsky, Maria V Zaretskaia

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: beta-amyloid toxicity, proteolytic digestion, membrane channel, intracellular ion disturbances, calcium homeostasis, intracellular pH, lysosome

Online: 4 August 2021 (13:19:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202108.0014.v1

The Aerob and Anaerob Performance Beta-Alanine Supplement and Ergogenic Aid Using to the Power Output Variables in Cyclists: A Cohort Studies Random Model Effect

Yeliz Kahraman

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Beta-alanine; supplementation; nutrition,; aerob and anaerob performance

Online: 2 August 2021 (10:18:40 CEST)

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Working Paper ARTICLE

WNT/β-catenin Signaling Promotes TGF-β-mediated Activation of Human Cardiac Fibroblasts by Enhancing IL-11 Production

Edyta Działo, Marcin Czepiel, Karolina Tkacz, Maciej Siedlar, Gabriela Kania, Przemysław Błyszczuk

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: cardiac fibroblasts; WNT3a; WNT5a; beta-catenin; TGF-beta signalling; IL-11; cardiac fibrosis

Online: 29 July 2021 (13:15:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0564.v1

In vivo Neuropharmacological Potential of Gomphandra tetrandra (Wall.) Sleumer and in-silico Study against β-Amyloid Precursor Protein

Md Saidur Rahman, Md Nazmul Hasan Zilani, Md. Aminul Islam, Md Munaib Hasan, Md. Muzahidul Islam, Farzana Yasmin, Partha Biswas, Akinori Hirashima, Md. Ataur Rahman, Md. Nazmul Hasan, Bonglee Kim

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Neurological activity; GC-MS; ADMET profile; Molecular Docking; Beta-amyloid precursor protein; Alzheimer’s disease

Online: 26 July 2021 (10:27:40 CEST)

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Working Paper ARTICLE

Hspb1 Is Essential for Inducing Resistance to Proteotoxic Stress In Beta-Cells

Vinícius M. Gomes, Rosangela A. M. Wailemann, Gabriel S. Arini, Talita C. de Oliveira, Daria Raquel Queiroz, Ancély F. dos Santos, Letícia F. Terra, Stephan Lortz, Leticia Labriola

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: heat shock proteins; Diabetes mellitus; beta-cells; endoplasmic reticulum stress; proteostasis; HSPB1; cytoprotection; apoptosis.

Online: 8 July 2021 (14:03:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202106.0343.v1

Beta-elimination of Hyaluronate by Red King Crab Hyaluronidase

Dmitrii Sliadovskii, Tatyana Ponomareva, Maxim Molchanov, Irina Pozdnyakova-Filatova, Maria Timchenko, Oleg Gusev, Evgeny Sogorin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Hyaluronoglucosaminidase; Hyaluronidase; Hyaluronic Acid; Hyaluronate; Eukaryota; Hepatopancreas; Transcriptome; Crustacea; Bacteria; beta-elimination; NMR

Online: 14 June 2021 (10:04:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0556.v1

Bacterial Extracellular DNA Promotes β-amyloid Aggregation

George Tetz, Victor Tetz

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Alzheimer’s disease; amyloid-beta; amyloid plaques; bacterial DNA; protein aggregation

Online: 24 May 2021 (10:33:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0418.v1

Dual Mode of Action of Acetylcholine on Cytosolic Calcium Oscillations in Pancreatic Beta and Acinar Cells in Situ

Nastja Sluga, Sandra Postic, Srdjan Sarikas, Ya-Chi Huang, Andraz Stožer, Marjan Slak Rupnik

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: pancreas tissue slices; acetylcholine; beta cell; acinar cell; Ca2+ oscillations

Online: 18 May 2021 (11:03:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202105.0137.v1

The Role of cAMP in Beta Cell Stimulus-Secretion and Inter-cellular Coupling

Andraž Stožer, Eva Paradiž Leitgeb, Viljem Pohorec, Jurij Dolenšek, Lidija Križančić Bombek, Marko Gosak, Maša Skelin Klemen

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cAMP; beta cells; stimulus-secretion coupling; intercellular coupling; PKA; Epac2A

Online: 7 May 2021 (09:52:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0095.v1

The microRNA Landscape of Acute Beta Cell Destruction in Type 1 Diabetic Recipients of Intraportal Islet Grafts

Geert Antoine Martens, Geert Stange, Lorenzo Piemonti, Jasper Anckaert, Zhidong Ling, Daniel Pipeleers, Frans Gorus, Pieter Mestdagh, Dieter De Smet, Jo Vandesompele, Bart Keymeulen, Sarah Roels

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: beta cell, type 1 diabetes, islet transplantation, biomarkers, microRNA

Online: 6 May 2021 (15:01:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0087.v1

Functional Characterization of the obesity-linked variant of the β3-adrenergic receptor

Esraa Haji, Saeed Al Mahri, Yumna Aloraij, Shuja Shafi Malik, Sameer Mohammad

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: G-Protein Coupled Receptors; beta-3-adrenergic receptor; receptor desensitization

Online: 6 May 2021 (13:16:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0064.v1

The Effect of cAMP and the Role of Epac2A During Activation, Activity, and Deactivation of Beta Cell Networks

Maša Skelin Klemen, Jurij Dolenšek, Lidija Križančić Bombek, Viljem Pohorec, Marko Gosak, Marjan Slak Rupnik, Andraž Stožer

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: pancreas; tissue slices; beta cells; calcium imaging; amplifying pathway; forskolin; Epac2A KO

Online: 5 May 2021 (13:34:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0032.v1

Cellular Prion Protein and Amyloid – β Oligomers in Alzheimer's Disease – There Are Connections?

Naomi Hachiya, Michał Fułek, Katarzyna Zajączkowska, Donata Kurpas, Elżbieta Trypka, Jerzy Leszek

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Alzheimer’s disease; cellular prion protein; amyloid β and PrP interaction in Alzheimer’s; BACE1; Aβ

Online: 5 May 2021 (10:45:04 CEST)

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Working Paper ARTICLE

Oxidase Reactivity of CuII Bound to N-truncated Aβ Peptides Promoted by Dopamine

Luigi Casella, Chiara Bacchella, Simone Dell'Acqua, Stefania Nicolis, Enrico Monzani

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: copper; amyloid-β peptides; Alzheimer’s disease; oxidative stress; dopamine; neurodegeneration.

Online: 26 April 2021 (13:23:52 CEST)

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Working Paper REVIEW

The Microbiota-Gut-Brain Axis and Alzheimer Disease. From Dysbiosis to Neurodegeneration: Focus on the Central Nervous System Glial Cells

Maria Grazia Giovannini, Daniele Lana, Chiara Traini, Maria Giuliana Vannucchi

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloid-β; endotoxin; short chain fatty acids; clasmatodendrosis; cytokines; neurovascular unit; vagus nerve; Toll-like Receptor 4

Online: 26 April 2021 (13:22:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0413.v2

Total Face Irregularity Strength of Type (Alpha, Beta, Gamma) of Grid Graphs

Abdul Aleem Mughal, Raja Noshad Jamil

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: Total Face labeling of type (Alpha, Beta, Gamma); Total face irregularity strength; artesian product of path graphs; Graph Labeling; Graph theory

Online: 21 April 2021 (12:46:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0270.v1

Barriers to Small Molecule Drug Discovery for Systemic Amyloidosis

Gareth Morgan

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Systemic amyloidosis; amyloid fibrils; amyloidogenesis inhibitors; antibody light chains; light chain stabilizers; doxycycline; EGCG; thioflavin T; filter trap; PAINS

Online: 9 April 2021 (15:07:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0137.v1

Gross Alpha and Gross Beta Activity Concentrations in Dust Fraction of Urban Surface Deposited Sediment in Russian Cities

Mohamed Y. Hanfi, Andrian A. Seleznev, Ilia Yarmoshenko

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: urban surface deposited sediments (USDS); gross alpha activity (GA); gross beta activity (GB); dust

Online: 5 April 2021 (13:00:40 CEST)

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Working Paper ARTICLE

Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla and 100 Khz Magic-Angle Spinning

Evgeny Nimerovsky, Kumar Tekwani Movellan, Xizhou Zhang, Marcel C. Forster, Eszter Najbauer, Kai Xue, Rıza Dervişoğlu, Karin Giller, Christian Griesinger, Stefan Becker, Loren B Andreas

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: magic-angle spinning; solid-state NMR; membrane protein; beta barrel; transmembrane; proton detection; high magnetic field

Online: 29 March 2021 (12:49:07 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202103.0478.v1

Mechanisms of Network Interactions for Flexible Cortico-Basal Ganglia-Mediated Action Control

Petra Fischer

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Gamma oscillations; beta oscillations; subthalamic nucleus; globus pallidus; movement control

Online: 18 March 2021 (12:19:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202103.0426.v1

Live Cell FRET Imaging Reveals Amyloid β-Peptide Oligomerization in Hippocampal Neurons

Yang Gao, Stefan Wennmalm, Bengt Winblad, Sophia Schedin-Weiss, Lars Tjernberg

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloid β-peptide; oligomerization; aggregation; FRET

Online: 16 March 2021 (13:29:35 CET)

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Preprint REVIEW | doi:10.20944/preprints202103.0365.v1

Neurofluids – Deep Inspiration, Cilia and Preloading of the Astrocytic Network

Hans Ludwig, Steffi Dreha-Kulaczewski, Christoph Bock

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Real-time MR imaging; CSF; cilia sensing; aquaporin; nitric oxide; amyloid-ß; glymphatic system; hydrocephalus

Online: 15 March 2021 (10:36:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202102.0520.v1

Structure and Dynamics of Meprin β in Complex with a Hydroxamate-Based Inhibitor

Miriam Linnert, Claudia Fritz, Christian Jäger, Dagmar Schlenzig, Daniel Ramsbeck, Martin Kleinschmidt, Michael Wermann, Hans-Ulrich Demuth, Stephan Schilling

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Meprin B; Meprin beta; metalloproteinase; astacin; hydroxamate; SAR (structure activity relationship); MWT-S-270

Online: 23 February 2021 (14:27:53 CET)

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Preprint REVIEW | doi:10.20944/preprints202102.0205.v1

Fate of β-Carotene within Loaded Delivery Systems in Food: State of Knowledge

Vaibhav Maurya, Amita Shakya, Manjeet Aggarwal, K.M. Gothandam, Torsten Bohn, Sunil Pareek

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Beta-carotene; bioavailability; delivery system; encapsulation; engineered nanomaterial; SLNs; NLCs

Online: 8 February 2021 (12:31:57 CET)

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Accruing evidence on the influence of β-carotene regarding the prevention of several chronic diseases - in addition to its well-acknowledged role in vision has been a strong driver for developing alternative delivery systems. Though oral delivery is accepted as the most fitting, mild and safe path for delivering bioactive agents, β-carotene delivery via food items poses challenges due to its lipophilic nature, poor water-solubility, high chemical/photochemical instability and poor oral bioavailability. Nanotechnology has opened new windows for delivering bioactive agents. Their physiochemical characteristics, i.e. small size, high surface area, unique composition, biocompatibility and biodegradability make these nanomaterials an attractive tool for β-carotene delivery. Delivering β-carotene through nanoparticles does not only improve its bioavailability/bioaccumulation in target tissues, but also lessens its sensitivity against environmental factors during processing. Regardless of these benefits, nanocarriers inherit some limitations, such as variations in sensory quality, modification of the food matrix, increasing costs, as well as limited consumer acceptance and regulatory challenges. This research area has been rapidly evolved, with a plethora of innovative nano-engineered materials, including micelles, nano/microemulsion, liposomes, niosomes, solid-lipid nanoparticles and nanostructured lipid carriers. These nano-delivery systems make conventional delivery systems appear archaic and promise better solubilization, protection during processing, improved shelf-life, higher bioavailability as well as controlled and targeted release. This review provides information on the state of knowledge on β-carotene nano-delivery systems adopted for developing functional foods: depicting their classification, composition, preparation methods, challenges, release-and absorption of β-carotene in the GIT and possible risks and future prospects.

Preprint REVIEW | doi:10.20944/preprints202101.0625.v1

Licensed Anti-Microbial Drugs Logical for Clinical Trials Against Pathogens Currently Suspected in Alzheimer's Disease

Leslie C. Norins

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Alzheimer’s disease; dementia, beta-amyloid; germ theory; drug development; clinical trials; herpes; spirochetes; Chlamydia pneumoniae; Porphyromonas gingivalis; toxoplasma; mycobacteria

Online: 29 January 2021 (13:57:18 CET)

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Preprint REVIEW | doi:10.20944/preprints202101.0314.v1

Development of Cryogenic Detectors for Neutrinoless Double Beta Decay Searches

Mattia Beretta, Lorenzo Pagnanini

Subject: Physical Sciences, Acoustics Keywords: Neutrinoless Double Beta Decay; Lepton Number Violation; Rare event search; Cryogenic detector

Online: 18 January 2021 (10:32:28 CET)

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Working Paper REVIEW

Interferons and Multiple Sclerosis: Lessons from 25 Years of Clinical and Real-World Experience with Intramuscular Interferon-Beta-1a (Avonex)

Stanley Cohan, Barry Hendin, Anthony Reder, Kyle Smoot, Robin Avila, Jason Mendoza, Bianca Weinstock-Guttman

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: multiple sclerosis; interferon; interferon beta-1a; COVID-19

Online: 11 January 2021 (11:07:12 CET)

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Preprint SHORT NOTE | doi:10.20944/preprints202101.0069.v1

The Nonagenarian Neutrino

C Sivaram, Arun Kenath, Kiren OV

Subject: Physical Sciences, Acoustics Keywords: neutrino; neutrino detection; beta decay

Online: 5 January 2021 (10:09:53 CET)

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Preprint HYPOTHESIS | doi:10.20944/preprints202012.0813.v1

Multiscale Analysis of the Amyloid Degradation Toxicity Hypothesis of Alzheimer’s Disease

Dmitry Zaretsky, Maria Zaretskaia

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Alzheimer’s disease; beta-amyloid; protease; membrane; membrane channel; lysosome; autophagy; mitochondrion

Online: 31 December 2020 (13:32:26 CET)

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Alzheimer's disease (AD) is the most common cause of dementia and affects millions of people around the world. Neuronal death in AD is initiated by the toxic action of oligomeric amyloid-β (Aβ) peptides. The formation of membrane channels by Aβ is a primary molecular action and does not require any other proteins. Channels are formed by short amyloid fragments faster and more frequently than by full-length peptides. The channel formation is dependent on an electrostatic interaction between a positively charged peptide and a negatively charged membrane. Negative membranes can be found in several locations of a cell – the inner leaflet of plasma membrane, mitochondria, and lysosomes, which all are well-known cellular targets in AD. Considering that the amyloid enters a cell by endocytosis and is exposed to lysosomal enzymes, we propose the amyloid degradation toxicity hypothesis. Endopeptidases degrade the endocytosed peptide. Produced fragments form membrane channels, which can transfer various ions (including protons) and even relatively large compounds. The neutralization of lysosomal content inactivates enzymes, which fails the whole system of recycling cellular content, including autophagy. The permeabilization of lysosomes could also lead to cell death through necrotic and apoptotic mechanisms. We discuss several mechanisms that describe how amyloid degradation products reach plasma and mitochondrial membranes, and form membrane channels. The pathogenesis of AD is discussed at various levels in a context of how the primary molecular mechanism of membrane channel formation could progress into the disease state. The discussion starts at the molecular level and extends to why the development of a disease takes years and is closely associated with aging. The proposed hypothesis offers an interpretation to several clinical observations such as the involvement of iron metabolism and an inverse association between developing Alzheimer's disease and cancer. Predictions about potential biomarkers and effectiveness of future treatments are discussed.

Working Paper ARTICLE

Effectiveness and Feasibility of Injectable Escherichia coli-Derived Recombinant Human Bone Morphogenetic Protein-2 on Anterior Lumbar Interbody Fusion at Lumbosacral Junction For Adult Spinal Deformity: A Clinical Pilot Study

Sang-Kyu Im, Ki Young Lee, Hae Seong Lim, Seung-Jin Yoo, Jung-Hee Lee

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: adult spinal deformity; lumbosacral junction; anterior lumbar interbody fusion; bone morphogenetic protein; beta-tricalcium phosphate

Online: 28 December 2020 (12:06:09 CET)

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Working Paper ARTICLE

Paraoxonase-1 and -3 Protein Expression in Brain of the tg2576 Mouse Model of Alzheimer’s Disease

Jose Gregorio Salazar, Judit Marsillach, Ingrid Reverte, Bharti Mackness, Michael Mackness, Jorge Joven, Jordi Camps, María Teresa Colomina

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: paraoxonases; oxidative stress; Alzheimer’s disease; brain; Tg2576 mice; astrocytes; hippocampus; amyloid-β; microglia; neurons

Online: 18 December 2020 (11:53:45 CET)

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Working Paper ARTICLE

In vivo-Like Hierarchical Coculture of Hepatocytes, Hepatic Stellate Cells, and Sinusoidal Endothelial Cells for TGF-b-Induced Early Liver Fibrosis Studies in vitro

Qiao You Lau, Fuad Gandhi Torizal, Marie Shinohara, Yasuyuki Sakai

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: fibrogenesis; hepatic stellate cells; coculture; transforming growth factor beta; oxygen tension

Online: 16 December 2020 (08:51:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202011.0385.v1

mySORT: A Web Framework by using Deconvolution Approach to Estimating Immune Cell Composition from Complex Tissues

Shu-Hwa Chen, Bo-Yi Yu, Wen-Yu Kuo, Ya-Bo Lin, Sheng-Yao Su, I-Hsuan Lu, Chung-Yen Lin

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Cancer; Immunotherapy; Deconvolution; Alpha diversity; Beta diversity; Precision medicine; Microenvironment; Single-cell RNA sequencing

Online: 13 November 2020 (14:18:40 CET)

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Cancer immunotherapy reaches a remarkable achievement in various cancer types and brings new possibilities to improve cancer patients’ long-term survival. However, outcomes vary from case to case, and the present protocol benefits a small fraction of patients. One notable factor is the tumor microenvironment, especially the immune cell components, that may reflect the immune response's status quo on site. Thus, understanding the content of infiltrating immune cells in tumors is not only for research interesting but also a crucial subject toward precision medicine. We implement an algorithm for resolving relative proportions of twenty-one immune cell subclasses from a human tissue profiled transcriptome by microarray technology to reach the goal above. By selecting gene features and then adopting ?-Support Vector Regression, we can construct a deconvolution model and resolve the immune cell context. The excellent consistency between the estimated values and the correct immune-cell composition further demonstrates this approach provides a more natural alternative to revealing samples' immune cell content and reliable results like recent single-cell technologies. Based on this algorithm, the web-based deconvolution tool implemented named mySORT provides a user-friendly interface for estimating the immune cell content by uploading gene expression profiling. We also present comprehensive visualization 2D/3D plots in mySORT so that users can easily make a comparison between different samples. Finally, we synthesized pseudo-bulk expression data from single-cell transcriptomic datasets of 17 melanoma and 16 head and neck cancer patients. The deconvolution results of microarray-based data in the previous study and synthetic pseudo-bulk data all proved the excellent performance of mySORT. We believe that mySORT can help researchers in all fields easily understand complex immune microenvironment. The website of mySORT is freely accessible on https://symbiosis.iis.sinica.edu.tw/mySORT/.

Preprint REVIEW | doi:10.20944/preprints202011.0380.v1

Bacteria, Lipopolysaccharides, Amyloid and the Role of Iron Dysregulation in Parkinson’s Disease

Marthinus Janse van Vuuren, Theodore Albertus Nell, Jonathan Ambrose Carr, Douglas B Kell, Etheresia Pretorius

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Bacteria; Lipopolysaccharides; Gingipains; Amyloid and Parkinson’s disease; α-Synuclein

Online: 13 November 2020 (13:22:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202011.0349.v1

Influence of Mesenchymal Stem Cells Niche in Their Ostegenic Fate on Different Surfaces

Arkaitz Mucientes, Eva Herranz, Enrique Moro, Aranzazu González-Corchón, María Jesús Peña-Soria, Lydia Abasolo, Luis Rodriguez-Rodriguez, Jose Ramon Lamas, Benjamin Fernandez-Gutierrez

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: mesenchymal stem cell; beta-tricalcium phosphate; hydroxyapatite; osteogenesis.

Online: 12 November 2020 (13:16:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202011.0315.v1

Lower Bound of Sectional Curvature of Manifold of Beta Distributions and Complete Monotonicity of Functions Involving Polygamma Functions

Feng Qi

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: necessary and sufficient condition; complete monotonicity; monotonicity; inequality; polygamma function; lower bound; sectional curvature; manifold; beta distribution; convolution theorem for the Laplace transforms and analytic techniques

Online: 10 November 2020 (13:26:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202011.0001.v1

Dual-Task Effect on Cognitive and Body Function, β-amyloid Levels in Alzheimer's Dementia Patients: a Randomized Controlled Trial

Seung Min Nam, SEONG-GIL KIM

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Alzheimer’s Disease; Task Performance; Cognition; Human Activities; Amyloid Beta Protein; Dementia

Online: 2 November 2020 (08:06:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202010.0274.v1

Antioxidants From the Class of Polyphenols, NAC and Vitamin C Act Differently on Amyloid Fibril Formation by Two Human Cystatins: Stefin B and Cystatin C.

Alma Jahic Mujkic, Samra Hasanbasic, Magda Tušek Žnidarič, Selma Berbic, Eva Zerovnik

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: protein aggregation; inhibition of amyloid fibril formation; antioxidants; polyphenols; cystatin C; NAC and vitamin C; amyloid fibrils; stefin B aggregation; TEM

Online: 13 October 2020 (10:49:14 CEST)

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