Version 1
: Received: 11 June 2023 / Approved: 13 June 2023 / Online: 13 June 2023 (08:41:31 CEST)
How to cite:
Ng, Y.; Tan, S.S.; Moy, F.M. Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera. Preprints2023, 2023060903. https://doi.org/10.20944/preprints202306.0903.v1
Ng, Y.; Tan, S.S.; Moy, F.M. Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera. Preprints 2023, 2023060903. https://doi.org/10.20944/preprints202306.0903.v1
Ng, Y.; Tan, S.S.; Moy, F.M. Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera. Preprints2023, 2023060903. https://doi.org/10.20944/preprints202306.0903.v1
APA Style
Ng, Y., Tan, S.S., & Moy, F.M. (2023). Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera. Preprints. https://doi.org/10.20944/preprints202306.0903.v1
Chicago/Turabian Style
Ng, Y., Seok Shin Tan and Foong Ming Moy. 2023 "Protocol of a Rapid Method to Quantify Beta-carotene in Human Sera" Preprints. https://doi.org/10.20944/preprints202306.0903.v1
Abstract
Beta-carotene is a fat-soluble antioxidant commonly found in foods such as fruits, vegetables, and palm oil. Despite various liquid chromatography methods are proposed to detect and measure the sera carotenoid level, a long retention time to elute this marker is needed. This study attempted to develop a rapid reverse phase method in eluding beta-carotene in human sera. The researchers managed to elute the antioxidant in 2.2 minutes by applying a combination of a C8 column, mobile phase comprising of acetonitrile mixed with methanol in 70:30 proportion, and an ultra-high performance liquid chromatography system. The outputs had a good calibration curve (R2=0.959) and low coefficient of variation (0.2%), suggesting this protocol is reliable. A column with a lower carbon chain such as C8 allows the beta-carotene molecule to flow through the column faster. Besides, selecting solvents with high elution strength coupled with an ultra-high performance liquid chromatography system which equipped with high pressure can force the beta-carotene through the column in a shorter duration compared to previous reported methods. Therefore, it is recommended to adopt this protocol in epidemiological studies where beta-carotene is screened as a dietary biomarker associated with disease of interest by using human sera in the population level.
Keywords
Rapid; beta-carotene; UHPLC; human sera; Malaysia
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
