Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Antioxidants From the Class of Polyphenols, NAC and Vitamin C Act Differently on Amyloid Fibril Formation by Two Human Cystatins: Stefin B and Cystatin C.

Version 1 : Received: 12 October 2020 / Approved: 13 October 2020 / Online: 13 October 2020 (10:49:14 CEST)

How to cite: Jahic Mujkic, A.; Hasanbasic, S.; Tušek Žnidarič, M.; Berbic, S.; Zerovnik, E. Antioxidants From the Class of Polyphenols, NAC and Vitamin C Act Differently on Amyloid Fibril Formation by Two Human Cystatins: Stefin B and Cystatin C.. Preprints 2020, 2020100274 (doi: 10.20944/preprints202010.0274.v1). Jahic Mujkic, A.; Hasanbasic, S.; Tušek Žnidarič, M.; Berbic, S.; Zerovnik, E. Antioxidants From the Class of Polyphenols, NAC and Vitamin C Act Differently on Amyloid Fibril Formation by Two Human Cystatins: Stefin B and Cystatin C.. Preprints 2020, 2020100274 (doi: 10.20944/preprints202010.0274.v1).

Abstract

We compare the effect on amyloid fibril formation by two homologous proteins from the family of cystatins, human stefin B (stB) and cystatin C (cysC) in presence of 3 polyphenols: curcumin, resveratrol and quercetin and 2 non-phenolic anti-oxidants: vitamin C (VitC) and N-acetyl cystein (NAC). Some of the experimental data have already been presented, here we compare, further discuss and highlight the results. The amyloid fibril formation was followed by ThT fluorescence and transmission electron microscopy. Inhibitory effects on amyloid fibrillation reaction depended on anti-oxidant class and concentration. The fact that different effect of polyphenols was observed with the two cystatins; Cur acted inhibitory on stB but not on cysC fibril formation, could be explained if the 3 polyphenols would not bind to the same binding site in the fibrils core. Other differences are pointed out and discussed. Synergistic effects of VitC and chosen polyphenols on amyloid fibrilllation of human stB have been explored and are reported here for the first time.

Subject Areas

protein aggregation; inhibition of amyloid fibril formation; antioxidants; polyphenols; cystatin C; NAC and vitamin C; amyloid fibrils; stefin B aggregation; TEM

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