Version 1
: Received: 17 December 2020 / Approved: 18 December 2020 / Online: 18 December 2020 (11:53:45 CET)
How to cite:
Salazar, J.G.; Marsillach, J.; Reverte, I.; Mackness, B.; Mackness, M.; Joven, J.; Camps, J.; Colomina, M.T. Paraoxonase-1 and -3 Protein Expression in Brain of the tg2576 Mouse Model of Alzheimer’s Disease. Preprints2020, 2020120459
Salazar, J.G.; Marsillach, J.; Reverte, I.; Mackness, B.; Mackness, M.; Joven, J.; Camps, J.; Colomina, M.T. Paraoxonase-1 and -3 Protein Expression in Brain of the tg2576 Mouse Model of Alzheimer’s Disease. Preprints 2020, 2020120459
Salazar, J.G.; Marsillach, J.; Reverte, I.; Mackness, B.; Mackness, M.; Joven, J.; Camps, J.; Colomina, M.T. Paraoxonase-1 and -3 Protein Expression in Brain of the tg2576 Mouse Model of Alzheimer’s Disease. Preprints2020, 2020120459
APA Style
Salazar, J.G., Marsillach, J., Reverte, I., Mackness, B., Mackness, M., Joven, J., Camps, J., & Colomina, M.T. (2020). Paraoxonase-1 and -3 Protein Expression in Brain of the tg2576 Mouse Model of Alzheimer’s Disease. Preprints. https://doi.org/
Chicago/Turabian Style
Salazar, J.G., Jordi Camps and María Teresa Colomina. 2020 "Paraoxonase-1 and -3 Protein Expression in Brain of the tg2576 Mouse Model of Alzheimer’s Disease" Preprints. https://doi.org/
Abstract
Background: Brain oxidative lipid damage and inflammation are common in neurodegenerative diseases such as Alzheimer’s disease (AD). Paraoxonase-1 and 3 (PON1 and PON3) protein expression have been described in tissues with no PON1 and PON3 gene expression. In the present study, we aimed to examine differences in PON1 and PON3 protein expression in the brain of a mouse model of AD. Methods: We used peroxidase-based and fluorescence-based immunohistochemistry in 5 brain regions (olfactory bulb, forebrain, posterior midbrain, hindbrain and cerebellum) of transgenic (Tg2576) mice with the Swedish mutation (KM670/671NL) responsible for a familial form of AD and corresponding wild-type mice. Results: We found intense PON1 and PON3 positive staining in star-shaped cells surrounding Aβ plaques in all Tg2576 mouse brain regions studied. Although we could not co-localize PON1 and PON3 with astrocytes, brain star-shaped cells, we found some co-localization of PON3 with microglia. Conclusions: These results suggest that 1) PON1 and PON3 cross the blood-brain barrier in discoidal HDLs and are transferred to specific brain cell types, and 2) PON1 and PON3 play an important role in preventing oxidative stress and lipid peroxidation in particular cell types, likely astrocytes and microglia, in AD pathology, and potentially in other neurodegenerative diseases
Medicine and Pharmacology, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
