Working Paper Article Version 1 This version is not peer-reviewed

Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla and 100 Khz Magic-Angle Spinning

Version 1 : Received: 27 March 2021 / Approved: 29 March 2021 / Online: 29 March 2021 (12:49:07 CEST)

How to cite: Nimerovsky, E.; Tekwani Movellan, K.; Zhang, X.; Forster, M.C.; Najbauer, E.; Xue, K.; Dervişoğlu, R.; Giller, K.; Griesinger, C.; Becker, S.; Andreas, L.B. Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla and 100 Khz Magic-Angle Spinning. Preprints 2021, 2021030691 Nimerovsky, E.; Tekwani Movellan, K.; Zhang, X.; Forster, M.C.; Najbauer, E.; Xue, K.; Dervişoğlu, R.; Giller, K.; Griesinger, C.; Becker, S.; Andreas, L.B. Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla and 100 Khz Magic-Angle Spinning. Preprints 2021, 2021030691

Abstract

The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1.2 GHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively.

Subject Areas

magic-angle spinning; solid-state NMR; membrane protein; beta barrel; transmembrane; proton detection; high magnetic field

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