Working Paper Article Version 1 This version is not peer-reviewed

Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla and 100 Khz Magic-Angle Spinning

Version 1 : Received: 27 March 2021 / Approved: 29 March 2021 / Online: 29 March 2021 (12:49:07 CEST)

A peer-reviewed article of this Preprint also exists.

Nimerovsky, E.; Tekwani Movellan, K.; Zhang, X.C.; Forster, M.C.; Najbauer, E.; Xue, K.; Dervişoǧlu, R.; Giller, K.; Griesinger, C.; Becker, S.; Andreas, L.B. Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning. Biomolecules 2021, 11, 752. Nimerovsky, E.; Tekwani Movellan, K.; Zhang, X.C.; Forster, M.C.; Najbauer, E.; Xue, K.; Dervişoǧlu, R.; Giller, K.; Griesinger, C.; Becker, S.; Andreas, L.B. Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning. Biomolecules 2021, 11, 752.

Journal reference: Biomolecules 2021, 11, 752
DOI: 10.3390/biom11050752

Abstract

The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1.2 GHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively.

Keywords

magic-angle spinning; solid-state NMR; membrane protein; beta barrel; transmembrane; proton detection; high magnetic field

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