Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Influence of Mesenchymal Stem Cells Niche in Their Ostegenic Fate on Different Surfaces

Version 1 : Received: 11 November 2020 / Approved: 12 November 2020 / Online: 12 November 2020 (13:16:15 CET)

How to cite: Mucientes, A.; Herranz, E.; Moro, E.; González-Corchón, A.; Peña-Soria, M.J.; Abasolo, L.; Rodriguez-Rodriguez, L.; Lamas, J.R.; Fernandez-Gutierrez, B. Influence of Mesenchymal Stem Cells Niche in Their Ostegenic Fate on Different Surfaces. Preprints 2020, 2020110349 (doi: 10.20944/preprints202011.0349.v1). Mucientes, A.; Herranz, E.; Moro, E.; González-Corchón, A.; Peña-Soria, M.J.; Abasolo, L.; Rodriguez-Rodriguez, L.; Lamas, J.R.; Fernandez-Gutierrez, B. Influence of Mesenchymal Stem Cells Niche in Their Ostegenic Fate on Different Surfaces. Preprints 2020, 2020110349 (doi: 10.20944/preprints202011.0349.v1).

Abstract

Bone innate ability to repair without scaring is surpassed by major bone damage. Current gold-standard strategies do not achieve a full recovery of bone biomechanical properties. To bypass these limitations, tissue engineering techniques based on hybrid materials made up of osteoprogenitor cells, like mesenchymal stem cells (MSCs), and bioactive ceramic scaffolds, like calcium phosphate-based (CaPs), are promising. Biological properties of the MSCs, are influenced by the tissue source. The aim of this study is to define the MSC source and construct (MSC and scaffold combination) most interesting for its clinical application in bone regeneration. iTRAQ generated the hypothesis that anatomical proximity to bone has a direct effect on MSC phenotype. MSCs were isolated from adipose tissue, bone marrow and dental pulp. MSCs were cultured both on plastic surface and on CaPs (hydroxyapatite and β-tricalcium phosphate) to compare their biological features. On plastic, MSCs isolated from dental pulp (DPSCs) were the MSCs with the highest proliferation capacity and the greatest osteogenic potential. On both CaPs, DPSCs are the MSCs with the greatest capacity to colonize bioceramics. Furthermore, results show a trend for DPSCs are the MSCs with the most robust increase in the ALP activity. We propose DPSCs as a suitable MSCs for bone regeneration cell-based strategies.What do you want to do ?New mailCopy

Subject Areas

mesenchymal stem cell; beta-tricalcium phosphate; hydroxyapatite; osteogenesis.

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.