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Preprint ARTICLE | doi:10.20944/preprints202001.0262.v1

Dispersion-Free Extraction of In(III) from HCl Solutions Using a Supported Liquid Membrane Containing HA324H+Cl- Ionic Liquid as Carrier

Francisco J. Alguacil, Félix A. López

Subject: Chemistry And Materials Science, Metals, Alloys And Metallurgy Keywords: indium(III); ionic liquid; supported, liquid membrane; Hostarex A324; transport

Online: 23 January 2020 (04:07:43 CET)

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Preprint ARTICLE | doi:10.20944/preprints202203.0229.v1

MALDI-TOF-MS-Based Identification of Monoclonal Murine Anti-SARS-CoV-2 Antibodies Within One Hour

Georg Tscheuschner, Melanie N. Kaiser, Jan Lisec, Denis Beslic, Thilo Muth, Maren Krüger, Hans-Werner Mages, Brigitte G. Dorner, Julia Knospe, Jörg A. Schenk, Frank Sellrie, Michael G. Weller

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: SARS-CoV-2 antibody; reproducibility crisis; peptide mass fingerprinting; monoclonal antibody; trace-ability; identity; antibody identification; antibody light chain; MALDI-TOF-MS

Online: 16 March 2022 (10:01:41 CET)

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During the SARS-CoV-2 pandemic, many virus-binding monoclonal antibodies have been developed for clinical and diagnostic purposes. This underlines the importance of antibodies as universal bioanalytical reagents. However, little attention is given to the reproducibility crisis that scientific studies are still facing to date. In a recent study, not even half of all research antibodies mentioned in publications could be identified at all. This should spark more efforts in the search for practical solutions for the traceability of antibodies. For this purpose, we used thirty-five monoclonal antibodies against SARS-CoV-2 to demonstrate how sequence-independent antibody identification can be achieved by simple means applied onto the protein. First, we examined the intact and light chain masses of the antibodies relative to the reference material NIST-mAb 8671. Already half of the antibodies could be identified based solely on these two parameters. In addition, we developed two complementary peptide mass fingerprinting methods with MALDI-TOF-MS that can be performed in 45 minutes and had a combined sequence coverage of over 80%. One method is based on the partial acidic hydrolysis of the protein by 5 mM of sulfuric acid at 99 °C. Furthermore, we established a fast way for a tryptic digest without an alkylation step. We were able to show that the distinction of clones is possible simply by a brief visual comparison of the mass spectra. In this work, two clones originating from the same immunization gave the same fingerprints. Later, a hybridoma sequencing confirmed the sequence identity of these sister clones. In order to automate the spectral comparison for larger libraries of antibodies, we developed the online software ABID 2.0 (https://gets.shinyapps.io/ABID/). This open-source software determines the number of matching peptides in the fingerprint spectra. We propose that publications and other documents critically relying on monoclonal antibodies with unknown amino acid sequences should include at least one antibody fingerprint. By fingerprinting an antibody in question, its identity can be confirmed by comparison with a library spectrum at any time and context.

Preprint ARTICLE | doi:10.20944/preprints202311.1192.v1

Disposition Kinetics of Cathinone and Its Metabolites after Oral Administration in Rats

Fahad Y. Sabei, Ibrahim Khardali, Mohamed A. Al-Kasim, Emad S. Shaheen, Magbool Oraiby, Ahmad Alamir, David Banji, Saeed Alshahrani, Abdulmajeed Jali, Mohammad Attafi, Mohammed Y. Albeishy, Ibraheem Attafi

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Cathinone; Cathine; Kinetics; HPLC-IT/MS; Disposition; Organ-specific response; UPLC-QTOF/MS

Online: 20 November 2023 (07:36:02 CET)

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Cathinone is a natural stimulant found in Catha edulis and commonly used in new psychoactive substances. The objective of this study was to examine the disposition kinetics and metabolic profile of cathinone and its metabolite cathine through a single oral dose of cathinone administration in rats. Cathine and cathinone concentrations were identified and quantified using ion trap liquid chromatography (LC-IT/MS), while the metabolic profile was determined using the quadrupole time of flight UPLC-QTOF/MS method in the serum, brain, lung, liver, kidney, and heart over a period of 0, 0.5, 2.5, 6, 12, 24, 48, and 72 hours. The findings revealed a dynamic interplay between the pharmacokinetics of cathinone and its impact on organ-specific biomarkers. The distribution of cathinone and cathine was determined in serum, brain, heart, lung, kidney, and liver. The highest concentration of cathinone was found in the kidney at 1438.6 ug/L. It gradually decreased to 1.97 within 48 hours and disappeared after 72 hours. Within half an hour, cathinone levels in the lungs, liver, and heart were found to be 859, 798.9, and 385.8 ug/L, respectively. However, within 2.5 hours, these levels decreased to 608.1, 429.3, and 309.1 ug/L and kept decreasing until they became undetectable after 24 hours. The concentration of cathinone in the rat brain reduces quickly, dropping to undetectable levels within six hours, with levels decreasing from 712.7 ug/L after just 30 minutes. In the brain and serum, cathine reached its highest levels at 2.5 hours, while in other organs, it peaked at 0.5 hours. This indicates that the conversion of cathinone to cathine is slower in the brain and serum. The study found that cathinone's pharmacokinetics have a significant impact on organ-specific biomarkers. After administering the cathinone, there was a sharp change in biomarker levels, particularly in the liver and brain, during the initial hours. It is essential to investigate the correlation between the changes in biomarkers found in the brain and the levels of cathinone and cathine. The results have significant implications for drug development, pharmacovigilance, and clinical practices involving cathinone. In conclusion, this comprehensive analysis of cathinone's impact on organ-specific biomarkers provides a basis for informed decision-making in medical practices and further research into the cathinone's pharmacological properties.

Preprint ARTICLE | doi:10.20944/preprints202309.1861.v1

Effect of Brewing Methods on Acrylamide Content and Antioxidant Activity Studying Eight Different Filter Coffee Preparation

Agnese Santanatoglia, Simone Angeloni, Davide Bartolucci, Lauro Fioretti, Gianni Sagratini, Sauro Vittori, Giovanni Caprioli

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Acrylamide; antioxidant activity; brewing methods; chlorogenic acids; coffee; HPLC-MS/MS; HPLC-DAD.

Online: 28 September 2023 (04:32:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0177.v1

Phytochemical Constituents Propolis Flavonoid, Immunological Enhancement, Anti-porcine Parvovirus Activities of isolated from the Propolis

Xia Ma, zhenhuan guo, zhiqiang zhang, xianghui li, yizhou lv, zhiqiang shen, Li Zhao, Yonglu Liu

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Propolis Flavonoid; UPLC-Q/TOF-MS/MS; immunological enhancement; Ferulic acid; Anti-PPV

Online: 4 November 2020 (08:50:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1144.v1

Behavior of FRP-Retrofitted Wall-Like RC Columns after Preloading to Simulate In-Service Conditions

HUSSEIN ELSANADEDY, Husain Abbas, TAREK ALMUSALLAM, Yousef Al-Salloum

Subject: Engineering, Civil Engineering Keywords: Wall-like RC columns; Strengthening; FRP; NSM rebars; Steel plates; Testing; FE mod- 29
eling; Preloading

Online: 17 November 2023 (15:40:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0864.v1

The Neutrino Mass Problem: From Double Beta Decay to Cosmology

Osvaldo Civitarese

Subject: Physical Sciences, Particle And Field Physics Keywords: neutrino mass; axions neutrino mass; propagator

Online: 11 May 2023 (13:45:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1274.v1

Evaporation of Small Sessile Drop Deposited on Horizontal Solid Surface: New Exact Solutions and Approximations

Peter Lebedev-Stepanov, Olga Savenko

Subject: Chemistry And Materials Science, Surfaces, Coatings And Films Keywords: sessile liquid droplet; evaporation rate; diffusion, Laplace equation, analytical solution, flux density, mass loss per unit surface area per unit time

Online: 17 August 2023 (11:39:17 CEST)

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Working Paper ARTICLE

On the Role of Cosmic Mass in Understanding the Relationships among Galactic Dark Matter, Visible Matter and Flat Rotation Speeds

U.V.S. Seshavatharam, S. Lakshminarayana

Subject: Physical Sciences, Astronomy And Astrophysics Keywords: Planck mass; Mach’s principle; distance cosmic mass; galactic visible mass; galactic dark mass; galactic flat rotation speeds; time dependent reference mass;

Online: 9 August 2021 (09:16:42 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202106.0119.v1

Customer Churn - Prevention Model – Recomendation Engine

JULIAN GRIJALBA FACUNDO

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: Churn 1; customer churn; customer segmentation; churn prevention; predictive churn 20 model; recommendation system engine

Online: 3 June 2021 (14:57:01 CEST)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202106.0113.v1

Customer Churn - Prevention Model – Prediction Model

Julian Eduardo Grijalba Facundo

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: Churn 1; customer churn; customer segmentation; churn prevention; predictive churn 20 model; recommendation system engine.

Online: 3 June 2021 (12:48:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0470.v1

Liquid-Liquid Extraction of Ferric Ions Into the Ionic Liquids

Katerina Cubova, Miroslava Semelova, Mojmir Nemec, Vit Benes

Subject: Chemistry And Materials Science, Inorganic And Nuclear Chemistry Keywords: ionic liquids; liquid-liquid extraction; iron extraction; 8-hydroxyquinoline; acetylacetone; thenoyltrifluoroacetone

Online: 25 November 2021 (11:58:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0348.v1

A Power Sequence Model as a Bridge between Fundamental Natural Constants and Macroscopic Liquid Systems

Otto G. Piringer

Subject: Physical Sciences, Mathematical Physics Keywords: coupling constants; natural constants; neutrino mass; Higgs mass; Z boson mass; proton mass; nuclear binding; interaction function

Online: 23 September 2022 (02:04:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0305.v1

Multi-Method Determination of Antioxidant Capacity, Phytochemical and Biological Investigation of Four Different Solvent Extractives of Leucophyllum frutescens (cenizo)

Imtiaz Ahmad, Saeed Ahmad, Huma Rao, Umer Shaukat, Muhammad Nadeem Shahzad, M. Sajid-ur- Rehman, Abdul Basit, M. Adeel Arshad, Bilal Ahmad

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Leucophyllum frutescens; Total Phenolic Contents (TPC); Total Flavonoid Content (TFC); Total Antioxidant Activity (TAA); DPPH; CUPRAC; FRAP; gas chromatography-mass spectrometry (GC-MS)

Online: 10 November 2020 (12:00:06 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202212.0070.v1

Diester Chlorogenoborate Complex: A New Naturally Occurring Boron-Containing Compound

Andrei Biţă, Ion Romulus Scorei, Nagendra Rangavajla, Ludovic Everard Bejenaru, Gabriela Rău, Cornelia Bejenaru, Maria Viorica Ciocîlteu, Laura Dincă, Johny Neamţu, Andrei Bunaciu, George Dan Mogoşanu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: diester chlorogenoborate complex; green coffee bean; HPTLC/UV; HPTLC/ESI–MS; UHPLC/MS; FTIR

Online: 5 December 2022 (09:08:41 CET)

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Working Paper ARTICLE

Determination of Eleven Veterinary Drugs in Chicken Meat and Liver

Amanda Barros, Caue Sousa Novo, Vivian Feddern, Arlei Coldebella, Gerson Neudi Scheuermann

Subject: Biology And Life Sciences, Food Science And Technology Keywords: broiler; feed additives; LC–MS/MS; meat legislation; meat safety; poultry meat; veterinary drugs

Online: 22 September 2021 (12:13:55 CEST)

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Working Paper ARTICLE

Assessments of Sugars, Organic Acids, Amino Acids, Polyphenols, Macro and Trace Elements from Fresh Tree Peony Flowers

Jiantao Zhao, Yao Xu, Zhijun Zhang, Zisha He, Weijie Lan, Jing Zhang

Subject: Chemistry And Materials Science, Food Chemistry Keywords: tree peony flowers; sugars and organic acids; amino acids; polyphenols; GC-MS; LC-MS

Online: 20 March 2019 (15:09:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202006.0111.v1

A Novel Family of Quadrupole-Orbitrap Mass Spectrometers for a Broad Range of Analytical Applications

Jan-Peter Hauschild, Amelia Corinne Peterson, Erik Couzijn, Eduard Denisov, Denis Chernyshev, Christian Hock, Hamish Stewart, Ralf Hartmer, Dmitry Grinfeld, Christian Thoeing, Bastian Reitemeier, Arne Kreutzmann, Oliver Lange, Wilko Balschun, Aivaras Venckus, Sebastian Kanngiesser, Alexander Kholomeev, Gregor Quiring, Frank Czemper, Eloy Wouters, Michael Belford, Mathias Mueller, Jens Grote, Tabiwang N. Arrey, Julia Kraegenbring, Kerstin Strupat, Siegrun Mohring, Catharina Crone, Alexander Harder, Aaron Robitaille, Aaron Gajadhar, Khatereh Motamed, Yang Liu, Daniel Lopez Ferrer, Nicolaie Eugen Damoc, Angela Criscuolo, Kristina Srzentic, Ed George, Charles Yang, Ioanna Ntai, Amanda Souza, Andreas Wieghaus, Alexander Makarov

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Orbitrap mass spectrometry; high resolution; accurate mass

Online: 8 June 2020 (10:17:36 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.1197.v1

Some Recent Advances on Germanium Recovery from Various Resources

Francisco Jose Alguacil, Jose Ignacio Robla

Subject: Chemistry And Materials Science, Metals, Alloys And Metallurgy Keywords: germanium; recovery; leaching; liquid-liquid extraction; adsorption; ion exchange ;

Online: 18 April 2024 (14:04:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1349.v1

Incorporation of Functionalized Amino Acid Ionic Liquid into Highly Porous MOF-177 to Improve Post-Combustion CO₂ Capture Capacity

Firuz A. Philip, Amr Henni

Subject: Engineering, Chemical Engineering Keywords: CO2 Capture; Metal Organic Framework (MOF); Ionic Liquid; Amino Acid Ionic Liquid (AAILs); Task-Specific Ionic Liquid (TSIL); MOF-177

Online: 20 September 2023 (10:40:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1752.v1

A Thermostable Micellar Formulation of Rapalogs for Intracerebroventricular Delivery and the Therapy of Neurological Disorders

Laura Gianessi, Alessandro Magini, Roberto Dominici, Stefano Giovagnoli, Diego Dolcetta

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Intracerebroventricular, ICV; mTOR, mTOR-I, rapalog; Tuberous Sclerosis, TSC; Multiple Sclerosis, MS; Experimental Autoimmune Encephalitis, EAE; Alzheimer’s Disease, AD; Thermostable Micelles, Micellar Liquid Formulation; Ommaya device

Online: 24 August 2023 (12:53:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1600.v1

Repurposing Castanea sativa By-Product Extract as a Potent Anti-inflammatory Agent

Luisa Frusciante, Michela Geminiani, Tommaso Olmastroni, Pierfrancesco Mastroeni, Alfonso Trezza, Laura Salvini, Stefania Lamponi, Ottavia Spiga, Annalisa Santucci

Subject: Biology And Life Sciences, Life Sciences Keywords: Castanea sativa; waste repurposing; polyphenols; UPLC-MS-MS; inflammation; RAW 264.7; molecular modeling; docking simulations

Online: 26 March 2024 (12:53:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints201807.0267.v1

Chemical characterization of craft Filuferru spirit from Sardinia, Italy

Ilaria Langasco, Gavino Sanna, Marco Caredda, Angelo Panzanelli, Maria Itria Pilo, Nadia Spano, Giacomo Petretto, Pietro Paolo Urgeghe

Subject: Chemistry And Materials Science, Food Chemistry Keywords: Filuferru; spirit; distillation; grape marc; volatile compounds; copper; trace elements; GC-MS; ICP-MS; PCA

Online: 16 July 2018 (09:23:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0313.v1

Melting, Solidification and Viscosity Properties of Multicomponent Fe-Cu-Nb-Mo-Si-B Alloys With Low Aluminum Addition

Yuri N. Starodubtsev, Vladimir S. Tsepelev, Viktor V. Konashkov, Nadezhda P. Tsepeleva

Subject: Chemistry And Materials Science, Metals, Alloys And Metallurgy Keywords: multicomponent alloys; melting; solidification; viscosity; liquid – liquid structure transition; clusters; activation energy

Online: 4 January 2024 (03:25:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1393.v1

A Computational Study of Hydrogen Dispersion and Explosion after Large-Scale Leakage of Liquid Hydrogen

Seong Yong Choi, Chang Bo Oh, Kyu Hyung Do, Byung-Il Choi

Subject: Engineering, Chemical Engineering Keywords: liquid hydrogen (LH2); dispersion; explosion; pseudo-source model; liquid pool model; hydrogen leakage

Online: 23 October 2023 (08:31:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1033.v1

Dual-Period Polarization-Dependent Diffraction Gratings Based on a Polymer-Stabilized Liquid Crystal

Marta Kajkowska, Miłosz Chychłowski, Sławomir Ertman, Piotr Lesiak

Subject: Physical Sciences, Optics And Photonics Keywords: liquid crystal, polymer, polymer-stabilized liquid crystal, dual-period diffraction grating, polariza-tion

Online: 17 October 2023 (09:33:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0085.v1

A New Simple Passage Culture to Improve the Viability of Bifidobacteria

Cuiqin Li, Lidan Dong, Ling Zhang, Laping He, Shunbin Qiao

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Bifidobacterium; growth inertia; solid-liquid-liquid passage culture; viable bacterium concentrations; rapid growth

Online: 1 August 2023 (10:45:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1001.v1

Study on Local Vibration Control of 100m X-BOW Polar Exploration Cruise Ship

Guo he Jiang, Jia chen Chen, Hao Guo, Gang Wu, Zhen zhen Liu

Subject: Engineering, Marine Engineering Keywords: polar exploration cruise; frequency response analysis; modal equivalent mass; tuned mass dampers; mass ratio

Online: 16 April 2024 (11:37:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0363.v1

Application of Liquid Chromatography Coupled to Mass Spectrometry for Direct Estimation of the Total Levels of Adenosine and Its Catabolites in Human Blood

Jakub Šofranko, Peter Mitro, Zora Lazúrová, Martin Jozef Péč, Tomáš Bolek, Renata Péčová, Matej Samoš, Radovan Murín

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: adenosine; inosine; xanthine; blood; LC-MS

Online: 6 February 2024 (09:48:20 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202203.0152.v1

Coated Blade Spray-Mass Spectrometry as a New Approach for the Rapid Characterization of Brain Tumors

Joanna Bogusiewicz, Magdalena Gaca-Tabaszewska, Dominik Olszówka, Karol Jaroch, Jacek Furtak, Marek Harat, Janusz Pawliszyn, Barbara Bojko

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: SPME; CBS-MS; brain tumors; lipidomics

Online: 10 March 2022 (14:16:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202010.0450.v1

Multiple Sclerosis in a Multi-Ethnic Population in Houston, Texas: A Retrospective Analysis

Vicki Mercado, Deepa Dongarwar, Kristen Fisher, Hamisu M. Salihu, George J. Hutton, Fernando X. Cuascut

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Multiple Sclerosis; MS; Disparities; Minority populations

Online: 22 October 2020 (09:03:44 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.0765.v1

The Contribution of Tumor Necrosis Factor to Multiple Sclerosis Progression Independent of Relapses

Valentina Mazziotti, Francesco Crescenzo, Ermanna Turano, Maddalena Guandalini, Maddalena Bertolazzo, Stefano Ziccardi, Federica Virla, Valentina Camera, Damiano Marastoni, Agnese Tamanti, Massimiliano Calabrese

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: TNF biology; MS pathology; PIRA; MS lesions; chronic compartmentalized inflammation; neurodegeneration; biomarkers; disease progression. 1. Introduction

Online: 10 January 2024 (09:54:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1548.v1

A One-step Sample Processing Method in Combination With Hplc-Ms/Ms for the Simultaneous Quantification of Atorvastatin, Ezetimibe and Three Metabolites Including O-Hydroxyl Atorvastatin, P-Hydroxyl Atorvastatin, and Ezetimibe-Glucuronide in Human Plasma

T.Nguyen Nguyen Le, Nai Ngoc Chuong, Tuan Duc Nguyen

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: atorvastatin; ezetimibe; o-hydroxyl atorvastatin; p-hydroxyl atorvastatin; ezetimibe-glucuronide; LC-MS/MS; SALLE; human plasma

Online: 21 June 2023 (12:06:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0071.v1

Lysophospholipids, Lysophosphatidic Acids and Monoacylglycerols: New Therapeutic Targets in Cardiovascular Diseases?

Thomas Duflot, Ly Tu, Matthieu Leuillier, Hind Messaoudi, Déborah Groussard, Saïda Azhar, Raphaël Thuillet, Marc Humbert, Vincent Richard, Christophe Guignabert, Jérémy Bellien

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: lysophospholipids; lysophosphatidic acids; cardiovascular diseases; HPLC-MS/MS; rodent models; pulmonary hypertension; chronic heart failure; hypertension

Online: 3 September 2021 (15:19:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201701.0036.v1

Optimization of Ultrasonic-Assisted Extraction by Response Surface Methodology with Maximal Phenolic Yield and Antioxidant Activity from Acer truncatum Leaves

Lingguang Yang, Peipei Yin, Hang Fan, Qiang Xue, Ke Li, Xiang Li, Liwei Sun, Yujun Liu

Subject: Biology And Life Sciences, Plant Sciences Keywords: Acer truncatum leaves; ultrasonic-assisted extraction; response surface methodology; phenolics; antioxidant activity; UPLC-QTOF-MS/MS

Online: 9 January 2017 (04:16:01 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0728.v1

Cell‐Free DNA as a Biomarker at Diagnosis and Follow up in 256 B and T Cell Lymphomas

Ramón Diez-Feijoo, Marcio Andrade-Campos, Joan Gibert, Blanca Sánchez-González, Lierni Fernández-Ibarrondo, Concepción Fernández-Rodríguez, Nieves Garcia-Gisbert, Laura Camacho, Marta Lafuente, Ivonne Vázquez, Lluís Colomo, Antonio Salar, Beatriz Bellosillo

Subject: Medicine And Pharmacology, Hematology Keywords: cfDNA; Liquid biopsy; Lymphoma; monitoring

Online: 11 December 2023 (15:59:03 CET)

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Preprint REVIEW | doi:10.20944/preprints202306.1940.v1

Liquid Biopsy in Neurological Diseases

Sunny Malhotra, Mari Carmen Martín Miras, Agustín Pappolla, Xavier Montalban, Manuel Comabella

Subject: Biology And Life Sciences, Life Sciences Keywords: microRNA; liquid biopsy; neurological diseases

Online: 28 June 2023 (03:26:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0525.v1

Nuclear Matter in 1+1 Dimensions

Robert Douglas Pisarski, Alexei Tsvelik, Marton Lajer, Robert M Konik

Subject: Physical Sciences, Nuclear And High Energy Physics Keywords: dense QCD; quarkyonic; Luttinger liquid

Online: 30 September 2021 (15:30:19 CEST)

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Preprint REVIEW | doi:10.20944/preprints202012.0495.v1

A Short Review on One Dimensional Wigner Crystallization

Niccolo Traverso Ziani, Fabio Cavaliere, Karina Guerrero Becerra, Maura Sassetti

Subject: Physical Sciences, Acoustics Keywords: Wigner crystal; Bosonization; Luttinger liquid

Online: 21 December 2020 (10:34:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1082.v1

Dynamic Analysis Method of Liquid-Filled Containers considering the Fluid-Structure Interaction Effect

Xi bing Fang, Xin Bao, Fengjiang Yue, Qiyuan Zhao

Subject: Engineering, Marine Engineering Keywords: fluid-structure interaction; acoustic fluid elements; liquid sloshing modes; liquid-filled containers; dynamic analysis

Online: 15 January 2024 (07:07:01 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1943.v1

Antibacterial Properties of Peptide and Protein Fractions from the Cornu aspersum Mucus

Lyudmila Velkova, Aleksandar Dolashki, Ventsislava Petrova, Emiliya Pisareva, Dimitar Kaynarov, Momchil Kermedchiev, Marya Todorova, Pavlina Dolashka

Subject: Biochemistry And Molecular Biology, Biology And Life Sciences Keywords: Cornu aspersum mucus; antimicrobial peptides; de novo MS/MS sequencing; proteomic analysis; mucus proteins whit antimicrobial action

Online: 29 April 2024 (16:47:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1785.v1

Dynamic Instrumental and Sensory Methods Used to Link Aroma Release and Aroma Perception: A Review

Jean-Luc Le Quéré, Rachel Schoumacker

Subject: Biology And Life Sciences, Food Science And Technology Keywords: in vivo; aroma release; aroma perception; dynamic methods; nosespace; DIMS; TI; TDS; TCATA; APCI-MS; PTR-MS

Online: 26 July 2023 (11:11:42 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202211.0165.v1

Data-Independent Acquisition (DIA) is Superior for High Precisely Phospho-Peptide Quantification in Fungi

Katharina Bersching, Thomas Michna, Stefan Tenzer, Stefan Jacob

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Proteomics; LC-MS/MS; phosphopeptide enrichment; bioinformatics; cellular signaling; Mag-naporthe oryzae; phosphorylation; DDA; DIA; phospho-peptidomics

Online: 9 November 2022 (02:09:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1394.v1

Methodology for Assessing the Technical Condition and Durability of Bridge Structures

Kostiantyn Medvediev, Anna Kharchenko, Anzhelika Stakhova, Vitalii Tsybulskyi, Yurii Yevseichyk, Adrián Bekö

Subject: Engineering, Civil Engineering Keywords: technical condition of the bridge; durability; reliability; residual resource; degradation; 20 predicting the bridge’ s condition ;

Online: 19 December 2023 (05:48:57 CET)

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Запропонована методика спрямована на визначення та прогнозування технічного стану елементів мосту, що може слугувати прогресивним інженерним інструментом для оцінки надійності та довговічності. Розроблено на основі фундаментальних досліджень, які узагальнюють досвід вивчення фізико-механічних і фізико-хімічних властивостей матеріалів мостових конструкцій, що експлуатуються в реальних умовах. Теоретичною основою методики є модель надійності та прогнозування залишкового ресурсу елементів мосту на основі теорії Маркова. Розроблена методика призначена для оцінки технічного стану окремих елементів мосту з подальшою комплексною оцінкою всієї конструкції. За показник технічного стану прийнята надійність при експлуатації. Цей кількісний показник надійності в моделі є критерієм для: оцінки рівня безпеки елементів мосту; ранжування елементів мосту за необхідністю для конкретних видів ремонту, реконструкції або заміни; стратегічне планування витрат на ремонт або реконструкцію в умовах обмеженого фінансування; прогнозування залишкового ресурсу елементів. Для застосування розробленої методики запропоновано алгоритм оцінки та прогнозування технічного стану мостів. Було проведено математичний експеримент розробленої методики, який підтвердив адекватність висунутої гіпотези, тобто використання моделі надійності та прогнозування залишкового ресурсу елементів мосту. По-перше, запропоновано триступеневий механізм уточнення технічного стану мосту, суттєво підвищення точності розрахунків. Тому розроблена методика має практичне значення і може слугувати основою для інформаційно-аналітичних систем управління станом мостів.

Preprint ARTICLE | doi:10.20944/preprints202305.1193.v1

Tiny Deep Learning Architectures Enabling Sensor-near Acoustic Data Processing and Defect Localization

Giacomo Donati, Federica Zonzini, Luca De Marchi

Subject: Engineering, Electrical And Electronic Engineering Keywords: Acoustic Emission Monitoring; Capsule Neural Network; Dilated Convolutional Neural 20 Network; Tiny Machine Learning; Time of Arrival Estimation

Online: 17 May 2023 (05:17:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0665.v3

Maternal Supply of Both Arachidonic and Docosahexaenoic Acids is Required for Optimal Neurodevelopment

Sanjay Basak, Rahul Mallick, Antara Banerjee, Surajit Pathak, Asim K. Duttaroy

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Arachidonic acid, 20:4n-6; Brain; Docosahexaenoic acid, 22:6n-3; Fetus; Maternal diet; Cognitive; Infants; Neurodevelopment; Neurogenesis

Online: 14 June 2021 (14:54:08 CEST)

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Preprint ESSAY | doi:10.20944/preprints202309.0606.v1

Based on UHPLC-Q-Orbitrap HRMS, the Effect of Chemical Composition before and after Fermentation of Gastrodia Tuder Halimasch Powder Was Compared

Yaning Wu, Hongwei Zhang, Zhenling Zhang, Jianguang Zhu, Songbo Ma, Yongqi Zhao, Yiming Wang, Jun Yuan, Xing Guo, Yajing Li, Shuai Zhang

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: gastrodia tuder halimasch powder; ultra high performance liquid chromatography tan- 34 dem quadrupole electrostatic field orbitrap high resolution mass spectrometry; stoichiometry; fer- 35 mentation processing

Online: 8 September 2023 (12:39:53 CEST)

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Working Paper ARTICLE

Mechanics of the Gyroscopic Precession and Calculation of the Galactic Mass

Jian Sun, Ting Xu, Zheng Jiao, Hui Wang

Subject: Physical Sciences, Acoustics Keywords: gyroscopic precession; solar system; galactic mass

Online: 25 May 2021 (11:16:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1436.v1

Stability of the Macrocyclic Gd-DOTA Contrast Agent (DOTAREM) under Different Estuarine Environmental Conditions

Ana Guerreiro, Pedro Brito

Subject: Environmental And Earth Sciences, Pollution Keywords: Gadolinium; Contrast Agent; ICP-MS; Chemical Stability

Online: 22 November 2023 (14:17:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1368.v1

Antioxidant and Antifungal Activities and Characterization of Phenolic Compounds Using HPLC- MS in Empetrum rubrum Vahl ex Willd.

Carlos Schneider, Makarena González-Reyes, Carola Vergara, Patricio Zapata

Subject: Biology And Life Sciences, Plant Sciences Keywords: Empetrum rubrum; Antioxidant; Antifungal activity; HPLC-MS

Online: 22 November 2023 (03:43:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1160.v2

Comparative Analysis of VOC Purification Techniques in Complex Cooking Emissions: Adsorption, Photocatalysis and Combined Systems

Daniele Zatta, Mattia Segata, Franco Biasioli, Ottaviano Allegretti, Giovanna Bochicchio, Roberto Verucchi, Francesco Chiavarini, Luca Cappellin

Subject: Chemistry And Materials Science, Food Chemistry Keywords: VOCs; PTR-MS; Adsorption; Photocatalysis; Cooking emissions

Online: 15 November 2023 (16:31:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.2130.v1

Comparative Study of Chemical Composition and Cholinesterase Inhibition Potential of Essential Oils Isolated from Artemisia Plants from Croatia

Olivera Politeo, Ivana Cajic, Anja Simic, Mirko Ruscic, Mejra Bektasevic

Subject: Biology And Life Sciences, Life Sciences Keywords: Artemisia; essential oil; GC-MS; AChE; BChE

Online: 30 September 2023 (08:17:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1985.v1

Diversity in Fruit Phytochemical Composition among Lagenaria siceraria Landraces

Lungelo Given Buthelezi, Sydney Mavengahama, Julia Sibiya, Charmaine Nontokozo Mchunu, Nontuthuko Rosemary Ntuli

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: medicinal plant; bioactive molecules; terpenoids; GC-MS

Online: 28 June 2023 (09:54:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201911.0363.v1

Morphology and Elemental Analysis of Freshly Emitted Particles from Packed-bed Domestic Coal Combustion

Masilu Daniel Masekameni, Tafadzwa Makonese, Isaac Tebogo Rampedi

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: physical properties; tem, icp-ms; elemental composition

Online: 28 November 2019 (12:05:34 CET)

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Preprint ARTICLE | doi:10.20944/preprints201903.0152.v1

GC-MS Investigation of Volatiles from Honeybee Worker Larvae and Larval Food at Different Instars

Haohao Zhang, Chunsheng Hou, Yongjun Liu, Pingli Dai, Yanyan Wu, Yonggang Pang, Qingyun Diao

Subject: Biology And Life Sciences, Insect Science Keywords: Apis mellifera; GC-MS; larva; terpenoids; volatiles

Online: 14 March 2019 (08:59:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints201902.0218.v1

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Essential Oil Salvia Officinalis in Sudan

Amna yousif Mohamed, Ahmed Ali Mustafa

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Salvia officinalis, Essential oil, GC-MS, Sudan

Online: 25 February 2019 (08:50:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints201610.0090.v1

Fatty Acids Profiles of Stipe and Blade from the Norwegian Brown Macroalga Laminaria hyperborea, Using Off-Line SPE and GC-MS

Lena Oksdøl Foseid, Hanne Devle, Yngve Stenstrøm, Carl Fredrik Naess-Andresen, Dag Ekeberg

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Laminaria hyperborean; lipids; fatty acids; GC-MS

Online: 21 October 2016 (08:59:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201905.0254.v1

A Brief Analysis of the Research Scheme of the Cyclotron Radiation from a Single Electron

Jian Ding

Subject: Physical Sciences, Nuclear And High Energy Physics Keywords: electromagnetic radiation; electron; charge-mass ratio; uncertainty principle; static mass

Online: 20 May 2019 (15:29:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201812.0011.v1

Solar and Atmospheric Neutrino Mass Splitting with SMASH Model

Chitta Ranjan Das, Katri Huitu, Timo Kärkkäinen

Subject: Physical Sciences, Particle And Field Physics Keywords: neutrino mass; Planck neutrino mass limit; Higgs metastability; SMASH model

Online: 3 December 2018 (08:09:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1953.v1

Reduction of Alternaria Toxins by Extrusion Processing of Whole Grain Red Sorghum Flour

Elizabet Janić Hajnal, Janja Babič, Lato Pezo, Vojislav Banjac, Bojana Filipčev, Jelena Miljanić, Jovana Kos, Breda Jakovac Strajn

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Co-rotating twin-screw extruder; Red sorghum flour; Alternariol; Alternariol monomethyl ether; Tenuazonic acid; Tentoxin; UPLC-MS/MS

Online: 26 December 2023 (08:48:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints201907.0005.v1

Screening of Lemon Balm Extracts for Anti-Aflatoxigenic, Antioxidant and Other Biological Activities

Efstathia Skotti, Nefeli Sophia Sotiropoulou, Iliada Lappa, Maria Kaiafa, Dimitrios Tsitsigiannis, Petros Tarantilis

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Melissa officinalis; antioxidant activity; aflatoxins; anti-aflatoxigenic activity; GC/MS; LC/MS; lemon balm; in situ; Pistachia Vera

Online: 1 July 2019 (11:16:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0168.v1

Impact of Reducing Statistically Small Population Sampling on 2 Threshold Detection in FBG Optical Sensing

Gabriel Cibira, Ivan Glesk, Jozef Dubovan, Daniel Benedikovič

Subject: Engineering, Telecommunications Keywords: statistically small population sampling; two-sided sampling; one-sided sampling; 29 threshold detection; fiber Bragg gratings; FBG sensing; optical spectrum analysis;

Online: 4 March 2024 (13:37:33 CET)

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Statistical denoising techniques have been well studied, however many challenges still remain. This 9 article focuses on determining the least necessary number of significant samples in a statistical pop-10 ulation sampling, while minimizing the impact on the statistical detection of the fiber Bragg grating 11 (FBG) power spectra. This is achieved by using Bayesian decision making, small population statis-12 tics while excluding false detection. First, we investigate the impact of two-sided sliding window 13 technique applied near the cell under the test. The window size varies from 8 to 60 samples. The 14 considered maximum size of population sampling is connected with the number of wavelength 15 samples within the bandwidth of the symmetrical FBG power spectra peaks. Regarding the sym-16 metry of FBG power spectra peaks and the possible reduction of computational demands, we inves-17 tigate the impact of a one-sided sliding window. This enables to achieve less processing latency for 18 real time applications thus brings benefits to FBG-based fiber optical sensing. Both, two- and one-19 sided small population sampling techniques are then experimentally verified. Detection results are 20 analyzed in depth via calculating detection thresholds by using the small size window sliding along-21 side the waveband. From results achieved, it was found that the normality three-sigma rule does 22 not need to be followed while using the small population sampling. Experimental demonstrations 23 and analyses showed that the proposed concept of denoising and statistical threshold detection 24 based on the sliding window does not depend on the prior knowledge of probability distribution 25 functions of the FBG power spectra peaks level and background noise. We have also found that the 26 thresholds’ adaptability mainly depends on the statistical numerical characteristics of the above ad-27 ditive mixture of the FBG power spectra peaks signals and background noise.

Preprint ARTICLE | doi:10.20944/preprints202304.0920.v1

Unveiling the Potential of BenzylethyleneAryl-Urea Scaffolds for Designing of new Onco Immunomodulating Agents

Raquel Gil-Edo, Santiago Royo, Miguel Carda, Eva Falomir

Subject: Biology And Life Sciences, Other Keywords: Aryl urea, angiogenesis, PD-L1, VEGFR-2, immune checkpoints, CD-11b, CD-80, THP-1, HT-29, co-cultures, tumor microenvironment.

Online: 25 April 2023 (10:23:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201907.0033.v1

Plasma Spectroscopy of Various Types of Gypsum: An ideal Terrestrial Analogue

Abhishek K. Rai, Jayanta K. Pati, Christian G. Parigger, Awadhesh K. Rai

Subject: Physical Sciences, Atomic And Molecular Physics Keywords: laser-induced plasma; atomic spectroscopy; laser-induced breakdown spectroscopy; 29 atomic spectroscopy; principal component analysis; partial least-square regression; gypsum; Mars

Online: 2 July 2019 (08:03:52 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202404.1865.v1

Detection and Characterization of Methylated Circulating Tumor DNAs in Gastric Cancer

Seung Young Seo, Sang Hee Youn, Jin-Han Bae, Sung-Hun Lee, Sun Young Lee

Subject: Biochemistry And Molecular Biology, Biology And Life Sciences Keywords: Gastric cancer; Liquid biopsy; MeDIP; Fragment

Online: 29 April 2024 (10:34:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0931.v1

Fully Automated Production of [68Ga]FAPI-46 with Gallium-68 from Cyclotron Using Liquid Targets

Alexandra I. Fonseca, Vitor H. Alves, Ivanna Hrynchak, Francisco Alves, Antero J. Abrunhosa

Subject: Chemistry And Materials Science, Inorganic And Nuclear Chemistry Keywords: radiopharmaceuticals; cyclotron; liquid targets; FAPI-46

Online: 14 September 2023 (10:31:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1949.v1

Evaluation of Physicochemical Separation Characteristics of Pig Manure According to the Type of Solid-Liquid Separator Processes

Soo-Ryang Kim, Seung-Hyun Park, Su-Chan Lee, Ji-Won Jung, Sang-Hyean Ahn, Kwon-Woong Ham, Myung-Gyu Lee

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: livestock; pig manure; solid–liquid separations

Online: 28 July 2023 (12:34:58 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202305.0314.v1

Cell-Free Methylated PTGER4 and SHOX2 Plasma DNA as a Biomarker for Therapy Monitoring and Prognosis in Advanced Stage NSCLC Patients

Michael Fleischhacker, Erkan Arslan, Dana Reinicke, Stefan Eisenmann, Gerit Theil, Jens Kollmeier, Christoph Schaeper, Christian Grah, Frank Klawonn, Stefan Holdenrieder, B. Schmidt

Subject: Biology And Life Sciences, Life Sciences Keywords: liquid profiling; plasma; methylation; mSHOX2; mPTGER4

Online: 5 May 2023 (07:23:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202303.0465.v1

Corrosion Performance of Commercial Alloys and Refractory Metals in Conditions for Electrorefining of Spent Nuclear Fuels

Yanhong Jia, Shuangshuang Chang, Xin Du, Shaoqiang Guo

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: Corrosion; Molten salts; Liquid cadmium; SEM

Online: 27 March 2023 (14:35:30 CEST)

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Preprint REVIEW | doi:10.20944/preprints202003.0032.v1

Adsorption Processing for the Removal of Toxic Hg(II) from Liquid Effluents: Advances in the 2019 Year

Francisco J. Alguacil, Félix A. López

Subject: Environmental And Earth Sciences, Environmental Science Keywords: mercury(II); adsorption; processing; liquid effluents

Online: 3 March 2020 (02:17:56 CET)

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Preprint ARTICLE | doi:10.20944/preprints201609.0049.v1

The Effect of 4-Octyldecyloxybenzoic Acid on Liquid-Crystalline Polyurethane Composites with Triple-Shape Memory and Self-Healing Properties

Jianfeng Ban, Linjiang Zhu, Shaojun Chen, Yiping Wang

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: shape memory; liquid crystalline; self-healing

Online: 15 September 2016 (11:07:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1011.v1

Simultaneous Analysis of Hydrophobic Atractylenolides, Atractylon and Hydrophilic Sugars in Bai-Zhu by HPLC Column Tandem Technique

Zhixing Gu, Xi Nie, Ping Guo, Yuehua Lu, Bo Chen

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: column tandem; reversed-phase liquid chromatography; hydrophilic interaction liquid chromatography; atractylodes macrocephala; atractylenolides and atractylon; sugars

Online: 15 September 2023 (05:34:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2178.v1

Predictive Modeling of Delay in an LTE Network by Optimizing the Number of Predictors Using Dimensionality Reduction Techniques

Mirko Stojčić, Milorad K. Banjanin, Milan Vasiljević, Dragana Nedić, Aleksandar Stjepanović, Dejan Danilović, Goran Puzić

Subject: Engineering, Telecommunications Keywords: delay; dimensionality reduction; LTE; VoIP; Neural Networks; Support Vector Machines; k-Nearest Neighbors; Feature Selection; Pareto 80/20 rule

Online: 30 June 2023 (07:38:35 CEST)

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Preprint REVIEW | doi:10.20944/preprints201911.0013.v1

Optimizing Nutrition Assessment to Create Better Outcomes in Lung Transplant Recipients: A Review of Current Practices

Mara Weber Gulling, Monica Schaefer, Laura Bishop-Simo, Brian C. Keller

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: lung transplantation; body mass index; nutrition; body composition; lean body mass; muscle mass; leptin; sarcopenia; creatinine-height index

Online: 1 November 2019 (13:02:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1760.v1

Taste-Active Peptides from Triple Enzymatically Hydrolyzed Straw Mushroom Proteins Enhance Salty Taste and Elucidate Its Effect on the Taste Receptor T1R1/T1R3 by Molecular Docking

Chuang Yu, Shiqing Song, Yunpeng Cheng, Jingyi Wangzhang, Min Sun, Tao Feng, Chi-Tang Ho, Qian Liu, Linyun Yao

Subject: Biology And Life Sciences, Food Science And Technology Keywords: straw mushroom; peptides from triple enzymatically hydrolyzed straw mushroom proteins; molecular docking; UPLC-Q-TOF-MS/MS; T1R1/T1R3

Online: 1 March 2024 (08:22:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202110.0415.v1

Reductions in Hydrogen Sulfide Precede Onset of Mitochondrial Quality Control in the Corneal Kindled Mouse Model of Epilepsy

Christi Cho, Maxwell Zeigler, Stephanie Mizuno, Richard S. Morrison, Rheem Totah, Melissa Barker-Haliski

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: epilepsy; hydrogen sulfide; corneal kindled mice, mitochondrial dysfunction, oxidative stress, LC-MS/MS; temporal lobe epilepsy; neurological disorder; gasotransmitter

Online: 27 October 2021 (13:36:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0162.v1

Relativistic Mass of Gravitational Field

yin zhu

Subject: Physical Sciences, Particle And Field Physics Keywords: Relativistic mass of gravitational field; Total mass of a galaxy; Constitution of the mass of a star with field

Online: 9 December 2021 (15:57:56 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1239.v1

Predicting the Need for Surfactant Replacement Therapy in Very Preterm Infants by Combining Gastric Fluid Metabolomics with Neonatal Data

Konstantia Besiri, Olga Begou, Konstantinos Lallas, Angeliki Kontou, Eleni Agakidou, Olga Deda, Helen Gika, Eleni Verykouki, Kosmas Sarafidis

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: gastric fluid; RDS; GC-MS; prematurity; surfactant; prediction

Online: 16 January 2024 (12:26:43 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1375.v1

A Rapid Method to Predict Beer Shelf Life Using an HS-SPME-MS e-NOSE

Ricard Boqué, Ana Carolina De Lima, Laura Aceña, Montserrat Mestres

Subject: Chemistry And Materials Science, Food Chemistry Keywords: HS-MS e-NOSE; packaging; prediction; shelf life

Online: 22 November 2023 (06:35:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0659.v1

Lumbar Pain in Patients with Multiple Sclerosis and Knowledge about Physiotherapeutic Methods of Combating

Martyna Odzimek, Waldemar Brola, Józef Opara

Subject: Public Health And Healthcare, Other Keywords: Low back pain (LBP); multiple sclerosis (MS); physiotherapy

Online: 9 November 2023 (14:51:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0198.v1

A Study of Gastric Fluid and Urine Metabolomics for the Prediction of Survival in Severe Prematurity

Konstantia Besiri, Olga Begou, Olga Deda, Evmorfia Bataka, Christos Nakas, Helen Gika, Angeliki Kontou, Eleni Agakidou, Kosmas Sarafidis

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: gastric fluid; urine; metabolite; GC-MS; prematurity; survival

Online: 4 May 2023 (05:21:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0468.v1

Suppressive Effects of Volatile Compounds from Bacillus spp. on Magnaporthe oryzae Triticum (MoT) Pathotype, Causal Agent of Wheat Blast

Musrat Zahan Surovy, Shahinoor Rahman, Michael Rostás, Tofazzal Islam, Andreas Von Tiedemann

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Bacillus; biocontrol; volatile organic compound; GC-MS; sporulation

Online: 18 April 2023 (03:28:47 CEST)

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Magnaporthe oryzae Triticum (MoT) pathotype is the causal agent of wheat blast, which has caused significant economic losses and threatens wheat production in South America, Asia and Africa. Three bacterial strains from rice and wheat seeds (B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) were used to explore the antifungal effects of volatile organic compounds (VOCs) of Bacillus spp. as a potential biocontrol mechanism against MoT. All bacterial treatments significantly inhibited both mycelial growth and sporulation of MoT in vitro. We found that this inhibition was caused by Bacillus VOCs in a dose-dependent manner. In addition, biocontrol assays using detached wheat leaves infected with MoT showed reduced leaf lesions and sporulation compared to the untreated control. VOCs from B. velezensis BTS-4 alone or a consortium (mixture of B. subtilis BTS-3, B. velezensis BTS-4, and B. velezensis BTLK6A) treatments consistently suppressed MoT in vitro and in vivo. Compared to the untreated control, VOCs from BTS-4 and Bacillus consortium reduced MoT lesions in vivo by 85% and 81.25%, respectively. A total of thirty-nine VOCs (from 9 different VOC groups) from 4 Bacillus treatments were identified by gas chromatography-mass spectrometry (GC-MS), of which 11 were produced in all Bacillus treatments. Alcohols, fatty acids, ketones, aldehydes, and S-containing compounds were detected in all four bacterial treatments. In vitro assays using pure VOCs revealed that hexanoic acid, 2-methylbutanoic acid, and phenylethyl alcohol are potential VOCs emitted by Bacillus spp. that are suppressive for MoT. The minimum inhibitory concentrations for MoT sporulation were 250 mM for phenylethyl alcohol and 500 mM for 2-methylbutanoic acid and hexanoic acid. Therefore, our results indicate that VOCs from Bacillus spp. are effective compounds to suppress the growth and sporulation of MoT. Understanding the MoT sporulation reduction mechanisms by Bacillus VOCs may provide novel options to manage the further spread of wheat blast by spores.

Preprint ARTICLE | doi:10.20944/preprints202304.0062.v1

Exosome Load Increases Systemically and Mediates Pro-tumoral M2 Macrophage Polarization in Lymphoma

Saima Syeda, Kavita Rawat, Anju Shrivastava

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Exosomes; lymphoma; LC-MS; pro-tumoral macrophages; cytokines

Online: 5 April 2023 (12:14:37 CEST)

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Macrophages are the key effector cells of innate immunity which show two polarized states: M1, classically activated and M2, alternatively activated. Tumor-associated macrophages (TAMs) which usually show M2 polarization, are immunosuppressive cells that enhance tumor metastasis and invasion. Also, enrichment of TAMs is known to be closely associated with poor prognosis of cancer patients. Therefore, TAMs are considered to be promising targets for immunotherapy. Importantly, tumor-derived exosomes emerged as a crucial player in immune regulation which can remodel the tumor microenvironment towards immunosuppressive state. Of note, few studies have shown that exosomes could induce polarization of macrophages towards M2 type in tumor condition, while others showed activation of M1 or mixed phenotype. Considering the role of TAMs in cancer, there is an urgent need to decipher the exosome-mediated cross-talk between tumor cells and macrophages.For this, we used murine model of Dalton’s lymphoma (DL), wherein, firstly, we evaluated increased exosome burden by assessing exosome level in serum as well as in various tissues of tumor-bearing host. Next, proteomic profiling of lymphoma-derived exosomes was done which revealed the presence of immunomodulatory proteins. Of note, these proteins were known to alter the macrophage polarization. In order to assess the effect of these exosomes on macrophages, we performed in vitro study using RAW264.7 cells. In vitro study revealed that frequent uptake of exosomes mediated a morphological change in macrophages which reduced their phagocytic activity. In parallel, exosomes increased reactive oxygen species (ROS) level and inhibited LPS-induced nitric oxide (NO) level in macrophages. Also, exosomes upregulated the expression of arginase-1 (an M2 marker) in macrophages but decreased the LPS-induced nitric oxide synthase 2 (NOS2) (an M1 marker) expression. Moreover, we observed a pro-tumoral cytokine profile in macrophages incubated with exosomes. Our study suggests that exosome load increases in lymphoma-bearing hosts systemically. Importantly, lymphoma-derived exosomes mediate the activation of macrophages towards pro-tumoral M2 type. Our results give an insight into the exosome-mediated tumor and immune cell cross-talk which could serve as an important prospect for targeting in cancer immunotherapy.

Preprint ARTICLE | doi:10.20944/preprints202111.0398.v1

Targeted Action and Molecular Interactions of Sarcin and Thionin With Aspergillosis Causing Aspergillus fumigatus

Ramya Ravindhiran, Ramya Krishnamurthy, Karthiga Sivarajan, JothiNayaki Sekar, Kumarappan Chidambaram, Kavitha Dhandapani

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Aspergillus giganteus; Aspergillosis; Antagonism; Sarcin; ADMET; GC-MS

Online: 22 November 2021 (13:56:36 CET)

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Working Paper ARTICLE

Metabolomics Response to Drought Stress in Morus notabilis variety Yu-711

Michael Ackah, Yisu Shi, Mengmeng Wu, Lei Wang, Guo Peng, Guo Liangliang, Jin Xin, Li Shaocong, Zhang Qiaonan, Changyu Qiu, Qiang Lin, Weiguo Zhao

Subject: Biology And Life Sciences, Plant Sciences Keywords: untargeted approach; metabolites; drought stress; mulberry, LC-MS

Online: 6 July 2021 (17:23:32 CEST)

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Preprint ESSAY | doi:10.20944/preprints202103.0058.v1

Mechanism of the Effect of High-Intensity Training on Urinary Metabolism in Female Water Polo Players Based on UHPLC-MS Non-Targeted Metabolomics Technique

Leilei Wang, Anping Chen, Jianying Li, Zhuo Sun, Shiliang Yan, Kaiyuan Xu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: high-intensity training; LC-MS; urine; water polo

Online: 2 March 2021 (09:37:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202101.0410.v1

Effects of Essential oils of Elettaria cardamomum Grown in India and Guatemala on Pseudomonas aeruginosa, Escherichia coli, and Gastrointestinal Disorders

Aftab Alam, Najeeb Ur Rehman, Mohd Nazam Ansari, Amber Palla

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Elettaria cardamomum; GC-MS, compositions; antibacterial; antidiarrhoeal; antispasmodic

Online: 21 January 2021 (09:13:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202009.0317.v1

Authentication and Traceability Study on Barbera d'Asti and Nizza DOCG Wines: The Role of Trace- and Ultratrace Elements

Maurizio Aceto, Federica Gulino, Elisa Calà, Elisa Robotti, Maurizio Petrozziello, Christos Tsolakis, Claudio Cassino

Subject: Chemistry And Materials Science, Food Chemistry Keywords: ICP-MS; trace elements; wine; Nizza; Barbera; authentication

Online: 14 September 2020 (00:49:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202008.0563.v1

Microplastic Presence in Sediment and Water of a Lagoon Bordering the Urban Agglomeration of Lagos, Southwest Nigeria

Oluwatosin Olarinmoye, Stock F., Scherf N., Whenu O.O., Asenime C.E., Ganzallo S.

Subject: Environmental And Earth Sciences, Pollution Keywords: Lagos lagoon; plastics; FTIR; pyrolysis GC-MS; sedimentology

Online: 26 August 2020 (06:30:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202007.0687.v1

Comparative Analysis of Volatile Compounds of Gamma-Irradiated Mutants of Rose (Rosa hybrida)

Jaihyunk Ryu, Dong-Gun Kim, Jung Min Kim, Jae Il Lyu, Yeong Deuk Jo, Si-Yong Kang, Jin-Baek Kim, Sang Hoon Kim

Subject: Chemistry And Materials Science, Applied Chemistry Keywords: Gamma-ray, Mutant, Rose, Volatile compounds, GC-MS

Online: 29 July 2020 (09:46:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201809.0122.v1

Spatial and Age Related Effects on Phytochemical Compounds of Ethanolic Extract of Gigantochloa scortechinii Rhizome

Johar Mohamed, Hazandy Abdul Hamid, Ahmad Ainuddin Nuruddin, Nik Muhammad Nik Abdul Majid

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: spatial; age-related; phytochemical; bamboo; rhizome; GC-MS

Online: 7 September 2018 (03:01:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201806.0142.v1

Fatty Acid Composition of Gluten-Free Food (Bakery Products) for Celiac People

Santino Orecchio, Antonella Maggio

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: gluten-free foods, fatty acids, GC-MS, celiac

Online: 8 June 2018 (16:20:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202009.0162.v2

Chaos, Order and Systematics in Evolution of the Genetic Code

Lei Lei, Zachary F. Burton

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloids; frozen accident; genetic code; hydrogels; liquid-liquid phase separation; mRNA; polyglycine; rRNA; ribosomes; translational fidelity; tRNA

Online: 21 October 2020 (10:48:18 CEST)

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Preprint DATA DESCRIPTOR | doi:10.20944/preprints202305.0963.v1

Proteomic Shift in Mouse Embryonic Fibroblasts Pfa1 during Erastin, ML210 and BSO-Induced Ferroptosis

Olga M. Kudryashova, Alexey M. Nesterenko, Dmitry A. Korzhenevskii, Valeriy K. Sulyagin, Vasilisa M. Tereshchuk, Vsevolod V. Belousov, Arina G. Shokhina

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Ferroptosis; proteomics; LC-MS/MS; mouse embryonic fibroblasts; Pfa1; cell death; glutathione peroxidase 4; erastin; ML210; L-Buthionine-sulfoximine; BSO

Online: 12 May 2023 (14:47:52 CEST)

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Working Paper ARTICLE

Banana Lectin from Musa paradisiaca is Mitogenic for Cow and Pig PBMC via IL-2 Pathway and ELF1

Roxane L. Degroote, Lucia Korbonits, Franziska Stetter, Kristina J. H. Kleinwort, Marie-Christin Schilloks, Barbara Amann, Sieglinde Hirmer, Stefanie M. Hauck, Cornelia A. Deeg

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: PBMC; polyclonal cell stimulation; complement pathway; plant lectin; Musa paradisiaca; banana lectin; BanLec; LC-MS/MS; proteomics; ELF1; IL-2

Online: 2 August 2021 (13:02:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints201807.0052.v1

Functional Metabolomics – a Useful Tool to Characterize Stress-Induced Metabolome Alterations Opening New Avenues Towards Tailoring Food Crop Quality

Corinna Dawid, Karina Hille

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Plant stress, abiotic stress, biotic stress, metabolomics, phytometabolomics, sensomics, phytohormonics, LC-MS/MS, NMR, targeted metabolomics, untargeted metabolomics, functional food.

Online: 3 July 2018 (14:18:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0897.v1

The Quantization of Mass

José Garrigues-Baixauli

Subject: Physical Sciences, Theoretical Physics Keywords: Quantization Mass; Planck Length

Online: 12 May 2023 (07:19:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.1841.v1

Inertial Propulsion Devices: A Review

Christopher G. Provatidis

Subject: Engineering, Mechanical Engineering Keywords: Inertial propulsion; Inertial drive; Out-of-balance mass; Rotational mass; Momentum conservation

Online: 26 January 2024 (08:28:56 CET)

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Preprint ARTICLE | doi:10.20944/preprints202303.0544.v1

Constraining Neutrino Mass in Dynamical Dark Energy Cosmologies With the Logarithm Parametrization and the Oscillating Parametrization

Rui-Yun Guo

Subject: Physical Sciences, Astronomy And Astrophysics Keywords: dynamical dark energy; neutrino mass; mass hierarchies of neutrinos; the observational constraints

Online: 31 March 2023 (07:21:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201902.0032.v1

A New Mass Measure and a Simplication of Modern Physics that Make Gravity Predictions Independent of G

Espen Gaarder Haug

Subject: Physical Sciences, Nuclear And High Energy Physics Keywords: Mass, Compton frequency, Planck length, Planck mass, Newton's gravitational constant, Schwarzschild radius

Online: 4 February 2019 (11:48:33 CET)

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