Version 1
: Received: 28 April 2024 / Approved: 29 April 2024 / Online: 29 April 2024 (16:47:15 CEST)
How to cite:
Velkova, L.; Dolashki, A.; Petrova, V.; Pisareva, E.; Kaynarov, D.; Kermedchiev, M.; Todorova, M.; Dolashka, P. Antibacterial Properties of Peptide and Protein Fractions from the Cornu aspersum Mucus. Preprints2024, 2024041943. https://doi.org/10.20944/preprints202404.1943.v1
Velkova, L.; Dolashki, A.; Petrova, V.; Pisareva, E.; Kaynarov, D.; Kermedchiev, M.; Todorova, M.; Dolashka, P. Antibacterial Properties of Peptide and Protein Fractions from the Cornu aspersum Mucus. Preprints 2024, 2024041943. https://doi.org/10.20944/preprints202404.1943.v1
Velkova, L.; Dolashki, A.; Petrova, V.; Pisareva, E.; Kaynarov, D.; Kermedchiev, M.; Todorova, M.; Dolashka, P. Antibacterial Properties of Peptide and Protein Fractions from the Cornu aspersum Mucus. Preprints2024, 2024041943. https://doi.org/10.20944/preprints202404.1943.v1
APA Style
Velkova, L., Dolashki, A., Petrova, V., Pisareva, E., Kaynarov, D., Kermedchiev, M., Todorova, M., & Dolashka, P. (2024). Antibacterial Properties of Peptide and Protein Fractions from the Cornu aspersum Mucus. Preprints. https://doi.org/10.20944/preprints202404.1943.v1
Chicago/Turabian Style
Velkova, L., Marya Todorova and Pavlina Dolashka. 2024 "Antibacterial Properties of Peptide and Protein Fractions from the Cornu aspersum Mucus" Preprints. https://doi.org/10.20944/preprints202404.1943.v1
Abstract
The study of biologically active substances from natural sources shows the prospect of obtaining a new type of antimicrobial agents, highly effective against pathogenic microorganisms and non-toxic to humans. Our study reveals for the first time that Cornu aspersum mucus has the potential to act as a source of antibacterial agents against bacterial pathogens such as Bacillus cereus 1085, Propionibacterium acnes 1897, Salmonella enterica 8691, Enterococcus faecalis 3915, and Enterococcus faecium 8754. Both mucus fractions with MW<20 kDa and MW>20 kDa showed antimicrobial activity and antioxidant properties that’s why they can be turned into an alternative agent to expensive synthetic antibacterial compounds. Using de novo sequencing 16 novel peptides with potential antibacterial activity were identified by in a fraction with MW<20 kDa. The results of electrophoretic and proteomic analysis of mucus fraction with MW >20 kDa presented proteins with potential antibacterial activity highly homologous to hemocyanins, mucus protein Aspernin, lectins, L-amino acid oxidase-like protein, and mucins (mucin-5AC, mucin-5B, mucin-2 and mucin-17). Thanks to the synergistic action of the bioactive components, the mucus fraction with MW > 20 kDa in low concentrations per 8–2 µg/mL showed promising antimicrobial activity against the tested bacteria, comparable to Vancomycin and without unwanted cytotoxic effects against non-target cells. Additionally, C. aspersum mucus fractions were found to have a positive effect on eukaryotic organisms by reducing the levels of intracellular oxidative damage and increasing the antioxidant capacity of the cell. These findings could serve as a basis for the development of a potent non-cytotoxic antibacterial agent to replace conventional antibiotics and avoid the development of antibiotic resistance.
Keywords
Cornu aspersum mucus; antimicrobial peptides; de novo MS/MS sequencing; proteomic analysis; mucus proteins whit antimicrobial action
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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