Version 1
: Received: 7 September 2023 / Approved: 8 September 2023 / Online: 8 September 2023 (12:39:53 CEST)
How to cite:
Wu, Y.; Zhang, H.; Zhang, Z.; Zhu, J.; Ma, S.; Zhao, Y.; Wang, Y.; Yuan, J.; Guo, X.; Li, Y.; Zhang, S. Based on UHPLC-Q-Orbitrap HRMS, the Effect of Chemical Composition before and after Fermentation of Gastrodia Tuder Halimasch Powder Was Compared. Preprints2023, 2023090606. https://doi.org/10.20944/preprints202309.0606.v1
Wu, Y.; Zhang, H.; Zhang, Z.; Zhu, J.; Ma, S.; Zhao, Y.; Wang, Y.; Yuan, J.; Guo, X.; Li, Y.; Zhang, S. Based on UHPLC-Q-Orbitrap HRMS, the Effect of Chemical Composition before and after Fermentation of Gastrodia Tuder Halimasch Powder Was Compared. Preprints 2023, 2023090606. https://doi.org/10.20944/preprints202309.0606.v1
Wu, Y.; Zhang, H.; Zhang, Z.; Zhu, J.; Ma, S.; Zhao, Y.; Wang, Y.; Yuan, J.; Guo, X.; Li, Y.; Zhang, S. Based on UHPLC-Q-Orbitrap HRMS, the Effect of Chemical Composition before and after Fermentation of Gastrodia Tuder Halimasch Powder Was Compared. Preprints2023, 2023090606. https://doi.org/10.20944/preprints202309.0606.v1
APA Style
Wu, Y., Zhang, H., Zhang, Z., Zhu, J., Ma, S., Zhao, Y., Wang, Y., Yuan, J., Guo, X., Li, Y., & Zhang, S. (2023). Based on UHPLC-Q-Orbitrap HRMS, the Effect of Chemical Composition before and after Fermentation of Gastrodia Tuder Halimasch Powder Was Compared. Preprints. https://doi.org/10.20944/preprints202309.0606.v1
Chicago/Turabian Style
Wu, Y., Yajing Li and Shuai Zhang. 2023 "Based on UHPLC-Q-Orbitrap HRMS, the Effect of Chemical Composition before and after Fermentation of Gastrodia Tuder Halimasch Powder Was Compared" Preprints. https://doi.org/10.20944/preprints202309.0606.v1
Abstract
Abstract：Objective: To compare the effects of fermentation on the chemical composition of Gastrodia Tuder Halimasch Powder, and to provide a basis for the processing, processing and clinical application of this medicinal material. Methods: UHPLC-Q-Orbitrap HRMS was used to identify compound structure by comparing excimer ions, characteristic fragment ions and reference substances, and referring to relevant literature and database search. The main differential components were screened by orthogonal partial least squares-discriminant analysis (OPLS-DA), and the differential components were quantitatively studied by high performance liquid chromatography, and the differences in the types and contents of chemical components before and after fermentation were compared. Results: A total of 77 compounds were identified or preliminarily derived after fermentation, including 21 terpenes, 18 organic acids, 9 flavonoids, 7 nucleosides, 6 amides, 4 amino acids, 2 pyrrolidone derivatives, 2 sterols, 2 alkaloids and 6 others.Through high performance liquid chromatography, it was found that the content of daidzein genistein and ergosterol was significantly increased after fermentation, and a new component ergosterol was produced. Compared with Gastrodia Tuder Halimasch Powder before fermentation, the content of genistin and other components was significantly reduced, and there may be a process of genistin conversion to genistein.Conclusion: The fermentation process will have a certain influence on the types and contents of the chemical components of Gastrodia Tuder Halimasch Powder, and ergosterol, genistein and .daidzein may be the main pharmacodynamic components, which can provide a useful reference for the clinical rational application, quality control and mechanism of action of Gastrodia Tuder Halimasch Powder.
Keywords
gastrodia tuder halimasch powder; ultra high performance liquid chromatography tan- 34 dem quadrupole electrostatic field orbitrap high resolution mass spectrometry; stoichiometry; fer- 35 mentation processing
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
