Version 1
: Received: 31 July 2023 / Approved: 1 August 2023 / Online: 1 August 2023 (10:45:31 CEST)
How to cite:
Li, C.; Dong, L.; Zhang, L.; He, L.; Qiao, S. A New Simple Passage Culture to Improve the Viability of Bifidobacteria. Preprints2023, 2023080085. https://doi.org/10.20944/preprints202308.0085.v1
Li, C.; Dong, L.; Zhang, L.; He, L.; Qiao, S. A New Simple Passage Culture to Improve the Viability of Bifidobacteria. Preprints 2023, 2023080085. https://doi.org/10.20944/preprints202308.0085.v1
Li, C.; Dong, L.; Zhang, L.; He, L.; Qiao, S. A New Simple Passage Culture to Improve the Viability of Bifidobacteria. Preprints2023, 2023080085. https://doi.org/10.20944/preprints202308.0085.v1
APA Style
Li, C., Dong, L., Zhang, L., He, L., & Qiao, S. (2023). A New Simple Passage Culture to Improve the Viability of Bifidobacteria. Preprints. https://doi.org/10.20944/preprints202308.0085.v1
Chicago/Turabian Style
Li, C., Laping He and Shunbin Qiao. 2023 "A New Simple Passage Culture to Improve the Viability of Bifidobacteria" Preprints. https://doi.org/10.20944/preprints202308.0085.v1
Abstract
Bifidobacterium is the most beneficial flora of the human gastrointestinal tract, but there is a concentration threshold for Bifidobacterium to exert its beneficial properties. In this work, the effect of several simple culture methods on the number of viable bifidobacteria was discussed to improve its viable concentrations, and we unexpectedly discovered that bifidobacteria have inert growth. The concentration of viable bacteria of two isolated Bifidobacterium animalis subsp. lactis, BZ11 and BZ25 and Lactobacillus plantarum SQ-4 were investigated using solid-liquid-liquid circulation subculture, solid-liquid-liquid subculture and solid-liquid-liquid circulation subculture. These 3 subculture methods increase the viable cell concentration, and the best is the solid-liquid-liquid subculture. The highest concentrations of third-generation BZ11 and BZ25 were 9.62 × 109 CFU/mL and 1.21 × 1010 CFU/mL in order by solid-liquid-liquid subculture method, which was 10.38 times and 42.31 times higher (P<0.05) than those of the first generation, respectively. However, none of these three subculture methods increased the concentration of SQ-4. These results suggested that Bifidobacterium has growth inertia and Solid-liquid-liquid passage culture inhibiting the growth inertia. This finding is valuable in promoting the industrial application of probiotic bifidobacteria to potential probiotic food production.
Biology and Life Sciences, Food Science and Technology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
