Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Targeted Action and Molecular Interactions of Sarcin and Thionin With Aspergillosis Causing Aspergillus fumigatus

Version 1 : Received: 20 November 2021 / Approved: 22 November 2021 / Online: 22 November 2021 (13:56:36 CET)

How to cite: Ravindhiran, R.; Krishnamurthy, R.; Sivarajan, K.; Sekar, J.; Chidambaram, K.; Dhandapani, K. Targeted Action and Molecular Interactions of Sarcin and Thionin With Aspergillosis Causing Aspergillus fumigatus. Preprints 2021, 2021110398 (doi: 10.20944/preprints202111.0398.v1). Ravindhiran, R.; Krishnamurthy, R.; Sivarajan, K.; Sekar, J.; Chidambaram, K.; Dhandapani, K. Targeted Action and Molecular Interactions of Sarcin and Thionin With Aspergillosis Causing Aspergillus fumigatus. Preprints 2021, 2021110398 (doi: 10.20944/preprints202111.0398.v1).

Abstract

Fungal infections are more predominant in agricultural and clinical fields. Aspergillosis caused by Aspergillus fumigatus leads to respiratory failure in patients along with various illnesses. Due to the limitation of antifungal therapy and antifungal drugs, there is an emergence to develop efficient antifungal compounds from natural sources to cure and prevent fungal infections. The present study deals with the investigation of the mechanism of active compounds from our candidate agonist Aspergillus giganteus for aspergillosis. The integrity of treated Aspergillus fumigatus cell membrane and nuclear membrane was analyzed by determining the release of cellular materials. The antagonistic potential of antifungal compounds on the pathogen was confirmed by SEM analysis. The effective concentration of antifungal compounds (AFCs) was found to be 250µg/ml. The GC-MS profiling has revealed the bioactive metabolites responsible for the antagonistic nature of Aspergillus giganteus. The bioavailability and toxicological properties of pathogenesis related proteins have proved the efficiency of pharmacokinetic properties of selected compounds. Interaction of sarcin, thionin, chitinase and its derivatives from Aspergillus giganteus with the virulence proteins of UDP-N-acetylglucosamine pyrophosphorylase, N-myristoyl transferase and Chitinase have proved the druggable nature of the antifungal compounds.

Keywords

Aspergillus giganteus; Aspergillosis; Antagonism; Sarcin; ADMET; GC-MS

Subject

MEDICINE & PHARMACOLOGY, Pharmacology & Toxicology

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