Preprint Essay Version 1 Preserved in Portico This version is not peer-reviewed

Mechanism of the Effect of High-Intensity Training on Urinary Metabolism in Female Water Polo Players Based on UHPLC-MS Non-Targeted Metabolomics Technique

Version 1 : Received: 27 February 2021 / Approved: 2 March 2021 / Online: 2 March 2021 (09:37:59 CET)

A peer-reviewed article of this Preprint also exists.

Wang, L.-L.; Chen, A.; Li, J.-Y.; Sun, Z.; Yan, S.-L.; Xu, K.-Y. Mechanism of the Effect of High-Intensity Training on Urinary Metabolism in Female Water Polo Players Based on UHPLC-MS Non-Targeted Metabolomics Technique. Healthcare 2021, 9, 381. Wang, L.-L.; Chen, A.; Li, J.-Y.; Sun, Z.; Yan, S.-L.; Xu, K.-Y. Mechanism of the Effect of High-Intensity Training on Urinary Metabolism in Female Water Polo Players Based on UHPLC-MS Non-Targeted Metabolomics Technique. Healthcare 2021, 9, 381.

Journal reference: Healthcare 2021, 9, 381
DOI: 10.3390/healthcare9040381

Abstract

Objective: To study the changes in urine metabolism in female water polo players before and after high-intensity training by using ultra-high performance liquid chromatography-mass spectrometry, and to explore the biometabolic characteristics of urine after training and competition. Methods: Twelve young female water polo players (except goalkeepers) from Shanxi Province were selected. A 4-week formal training was started after one week of acclimatization according to experimental requirements. Urine samples (5 ml) were collected before formal training, early morning after 4 weeks of training, and immediately after 4 weeks of training matches, and labeled as T1, T2, and T3, respectively. The samples were tested by LC-MS after pre-treatment. XCMS, SIMCA-P 14.1, and SPSS16.0 were used to process the data and identify differential metabolites. Results: On comparing the immediate post-competition period with the pre-training period (T3 vs T1), 24 differential metabolites involved in 16 metabolic pathways were identified, among which niacin and niacinamide metabolism and purine metabolism were potential post-competition urinary metabolic pathways in the untrained state of the athletes. On comparing the immediate post-competition period with the post-training period (T3 vs T2), 10 metabolites involved in 3 metabolic pathways were identified, among which niacin and niacinamide metabolism was a potential target urinary metabolic pathway for the athletes after training. Niacinamide, 1-methylnicotinamide, 2-pyridone, L-Gln, AMP, and Hx were involved in two metabolic pathways before and after the training. Conclusion: Differential changes in urine after water polo games are due changes in the metabolic pathways of niacin and niacinamide.

Subject Areas

high-intensity training; LC-MS; urine; water polo

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