Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Coated Blade Spray-Mass Spectrometry as a New Approach for the Rapid Characterization of Brain Tumors

Joanna Bogusiewicz
ORCID logo ,
Magdalena Gaca-Tabaszewska
ORCID logo ,
Dominik Olszówka
,
Karol Jaroch
ORCID logo ,
Jacek Furtak
,
Marek Harat
,
Janusz Pawliszyn
,
Barbara Bojko
* ORCID logo
Version 1 : Received: 8 March 2022 / Approved: 10 March 2022 / Online: 10 March 2022 (14:16:39 CET)

A peer-reviewed article of this Preprint also exists.

Bogusiewicz, J.; Gaca-Tabaszewska, M.; Olszówka, D.; Jaroch, K.; Furtak, J.; Harat, M.; Pawliszyn, J.; Bojko, B. Coated Blade Spray-Mass Spectrometry as a New Approach for the Rapid Characterization of Brain Tumors. Molecules 2022, 27, 2251. Bogusiewicz, J.; Gaca-Tabaszewska, M.; Olszówka, D.; Jaroch, K.; Furtak, J.; Harat, M.; Pawliszyn, J.; Bojko, B. Coated Blade Spray-Mass Spectrometry as a New Approach for the Rapid Characterization of Brain Tumors. Molecules 2022, 27, 2251.

DOI: 10.3390/molecules27072251

Abstract

Brain tumors are neoplasms with one of the highest mortality rates. Therefore, the availability of methods that allow the quick and effective diagnosis of brain tumors and selection of appropriate treatments is of critical importance for patient outcomes. In this study, coated blade spray-mass spectrometry (CBS-MS), which combines the features of microextraction and fast ionization methods, is applied for the analysis of brain tumors. In this approach, a sword-shaped probe is coated with a sorptive material to enable the extraction of analytes from biological samples. The analytes are then desorbed using only a few microliters of solvent, followed by the insertion of the CBS device into the interface on the mass spectrometer source. The results of this proof-of-concept experiment confirmed that CBS coupled to high-resolution mass spectrometry (HRMS) enables the rapid differentiation of two histologically different lesions: meningiomas and gliomas. Moreover, quantitative CBS-HRMS/MS analysis of carnitine, the endogenous compound, previously identified as discriminating metabolite showed good reproducibility with the variation below 10% when using a standard addition calibration strategy and deuterated internal standards for correction. The resultant data show that the proposed CBS-MS technique can be useful for on-site qualitative and quantitative assessments of brain tumor metabolite profiles.

Keywords

SPME; CBS-MS; brain tumors; lipidomics

Subject

CHEMISTRY, Analytical Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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