The study comprehensively evaluated the prognostic roles of PLR, NLR, MLR, BLR, and ELR in patients with acute exacerbation chronic obstructive pulmonary disease (AECOPD). 619 patients with AECOPD and 300 healthy volunteers were retrospectively included into the study. The clinical characteristics containing laboratory findings of the AECOPD patients and the blood cell counts (CBCs) of the healthy volunteers were collected. Compared with the healthy volunteers, PLR, NLR, and MLR were elevated in COPD patients in stable condition, and were further ele-vated during exacerbation. ELR showed the opposite trend. PLR, NLR, and MLR were all posi-tively correlated with hospital LOS as well as CRP. In contrast, ELR was negatively correlated with hospital LOS as well as CRP. Elevated PLR, NLR, and MLR were all associated with more serious airflow limitation in AECOPD. Elevated PLR, NLR, and MLR were all associated with increased in-hospital mortality while Elevated ELR was associated with decreased in-hospital mortality in AECOPD. A nomogram was construct to predict in-hospital mortality in AECOPD. The nomo-gram had a C-index of 0.850 (95% CI: 0.799 – 0.901) with good predictive value and clinical ap-plicability. In summary, PLR, NLR, MLR, and ELR served as predictors for clinical outcomes in patients with AECOPD.

Monochromatic documents claim for much less computer bandwidth for network transmission and storage space than their color or even grayscale equivalent. The binarization of historical documents is far more complex than recent ones as paper aging, color, texture, translucidity, stains, back-to-front interference, kind and color of ink used in handwritting, printing process, digitalization process, etc. are some of the factors that affect binarization. This article presents a new binarization algorithm for historical documents. The new global filter proposed is performed in four steps: filtering the image using a bilateral filter, splitting image into the RGB components, decision-making for each RGB channel based on an adaptive binarization method inspired by Otsu's method with a choice of the threshold level, and classification of the binarized images to decide which of the RGB components best preserved the document information in the foreground. The quantitative and qualitative assessment made with 21 binarization algorithms in three sets of “real world” documents showed very good results.

Multiple Gateways (GWs) provide network connectivity to Internet of Things (IoT) sensors in a Wide Area Network (WAN). The End Nodes (ENs) can connect to any GW by discovering and acquiring its periodic beacons. This provides GW diversity and hence improves coverage. If the GWs are within vicinity to each other, and they keep sending periodic beacons to be discovered, the result is interference and collisions. In this study, the impact of such intra-network interference is analyzed to determine the maximum number of GWs that can co-exist within a given urban neighborhood. The paper shows the relationship between beacon collisions and the resulting packet loss rates in a given urban environment. The expected collision rates are calculated for the Medium Access Control (MAC) layer. The eventual packet loss rates are calculated with the MAC and Physical (PHY) layers combined. For this purpose, a new co-channel interference model is presented. The model calculates the average Carrier to Interference ratio (C/I) averaged across all the colliding events. It takes into account the partial overlap durations and the relative power of each colliding signal. The model shows that a collision does not always cause a packet loss. Packet losses occur if there is a collision and if the C/I is below a threshold. The performance evaluation is presented using a combination of analytical and simulation methods. The analytical methods provide the theoretical foundation, and have been used to validate the simulation results. Furthermore, the system models are developed from experimental data obtained from measurements with real hardware. Numerical results are provided with a low bit rate Gaussian Frequency Shift Keying (GFSK) modulation at the PHY layer, which is derived from the IEEE 802.15.4 standard specification. This paper provides guidance on selecting the right GFSK modulation parameters for such low bit-rate and narrow bandwidth IoT applications. The analysis and simulation results show that larger beacon intervals help in reducing beacon loss rates, at the cost of larger beacon acquisition latency. On the flip side, the gateway discovery latency reduces with increasing GW density thanks to abundance of beacons.

: Inflammation may mediate the association of mitral valve prolapse (MVP) with ventricular scarring and arrhythmia. The purpose of this study is to investigate the association of neutrophil- and platelet-to-lymphocyte ratios (NLR, PLR) with ventricular arrhythmia in MVP. Clinical and demographic data were extracted from electronic medical records for all patients aged 18-90 years with MVP on echocardiography between 2016-19 at our institution. We studied 65 patients who had a differential blood count within six months prior to ambulatory ECG (A- ECG) (medi-an age [IQR] 70 [61-77] years; 62% female; 12% African American). Patients with surgery or in-fection within six months prior to CBC were excluded. Complex or frequent ventricular ectopy (cfVE) was defined as the presence of two or more premature ventricular contractions (PVCs) on a 10-second ECG tracing or greater than 1% PVC burden or NSVT on ambulatory ECG monitoring. Logistic and bivariate regression was used to analyze clinical, electrocardiographic and echo-cardiographic factors associated with NLR and PLR. Factors associated with higher NLR were congestive heart failure (p=0.005), male sex (p=0.01), age (p=0.003), and bileaflet MVP (p=0.04). NLR was associated with higher absolute PVC burden (rs=0.28, p=0.03) and cfVE (median NLR [IQR] 3.0 [1.6 – 6.3]) with cfVE versus (2.4 [1.4-3.4], p=0.04) without cfVE. An NLR of 5.6 yielded 50% sensitivity and 90% specificity for PVC burden >1% (AUC 0.69). No significant association was found between PLR and PVC burden or cfVE. NLR is associated with PVC burden and com-plex ventricular ectopy in MVP, suggesting that inflammation is a significant factor in the patho-genesis of ventricular arrhythmia in this condition.

Intelligent transportation systems (ITS) have recently evolved rapidly, which requires development of highly trustworthy and effective communication technologies for uses, including vehicle-to-vehicle (V2V) and vehicle-to-everything (V2X) communication; where Orthogonal Frequency Division Multiplexing (OFDM) is regarded as a strong candidate and highly popular option technique among these methods. However, the movement of vehicles introduces Doppler frequencies which produce inter-carrier interference (ICI), that is frequently occurs in V2X channels. This interference has the potential to compromise the integrity of subcarrier orthogonality within OFDM, leading to lower-quality communication and an increased likelihood of data transmission errors. When employing channels with doubly dispersive fading, OFDM necessitates the usage of a complex equalization based on the minimum mean-square error (MMSE) equalizer, which requires channel matrix inversion. Several low-complexity equalizers for OFDM have been developed and are based on band factorization, time domain LSQR (Least-Square QR) iterative computing, and banded minimum mean squared error (BMMSE). This paper proposes Conjugate Gradient Least Squares (CGLS), which is a novel iterative computation algorithm integrated with nonlinear equalizers. The suggested nonlinear equalization technique determines the trade-off between computations and performance. According to simulation data, the suggested nonlinear equalizer performs better than the current BMMSE and linear CGLS algorithms across doubly dispersive fading channels.

Neutrophil to lymphocyte ratio (NLR) is a promising predictive and prognostic factor in breast cancer. We investigated its ability to predict disease-free survival (DFS) and overall survival (OS) in patients with luminal A or luminal B-HER2-negative breast cancer who received neoadjuvant chemotherapy (NACT). Pre-treatment complete blood cell counts from 168 consecutive patients with luminal breast cancer were evaluated to assess NLR. The study population was stratified into NLRlow or NLRhigh according to a cut-off value established by receiving operator curve (ROC) analysis. Data on additional pre- and post-treatment clinical-pathological characteristics were also collected. Kaplan-Meier curves, log-rank tests, and Cox proportional hazards models were used for statistical analyses. Patients with pre-treatment NLRlow showed a significantly shorter DFS (HR 6.97, 95% CI 1.65-10.55, p= 0.002) and OS (HR 7.79, 95% CI 1.25-15.07, p= 0.021) compared to those with NLRhigh. Non-ductal histology, luminal B subtype, and post-treatment Ki67≥ 14% were also associated with worse DFS (p= 0.016, p= 0.002, and p= 0.001, respectively). In multivariate analysis, luminal B subtype, post-treatment Ki67≥ 14%, and NLRlow remained independent prognostic factors for DFS, while only post-treatment Ki67≥ 14% and NLRlow affected OS. The present study provides evidence that pre-treatment NLRlow helps identify women at higher risk of recurrence and death among patients affected by luminal breast cancer treated with NACT.

Ustekinumab, a new anti-interleukin-12/23 antibody, is an effective treatment for ulcerative colitis; however, data regarding predictive factors of its efficacy are limited. This study aimed to identify biomarkers that can predict the long-term outcome of ustekinumab treatment. We retrospectively reviewed the records of patients with active ulcerative colitis treated with ustekinumab at our hospital from June 2020 to January 2023. We divided patients into non-remission and remission groups, and examined whether baseline biomarkers, including C-reactive protein-to-lymphocyte ratio, and early treatment response could predict clinical remission at week 48 of ustekinumab treatment. Of the 33 patients included in the study, 21 (63.6%) were in clinical remission at week 48 of ustekinumab treatment. The baseline C-reactive protein-to-lymphocyte ratio value was identified as an independent prognostic factor for clinical remission at week 48 (odds ratio: 10, 95% confidence interval: 1.6–62.4, p=0.014), with the cutoff value of 3.353 showing excellent prognostic performance (sensitivity: 71.4%, specificity: 83.3%). Furthermore, the clinical response at week 4 (p=0.009) and that at week 8 (p=0.005) were significantly associated with clinical remission at week 48. The baseline C-reactive protein-to-lymphocyte ratio value and early treatment response are useful biomarkers to predict the long-term efficacy of ustekinumab treatment.

The ratio of males to females with ASD is generally quoted as 4:1 though it is believed that there are biases preventing females being diagnosed and that the true ratio is lower. These biases have not been clearly identified or quantified. Starting with a clinical dataset of 1711 children <18 years old four different methods were employed in an inductive study to identify and quantify the biases and calculate the proportion of females missed. A mathematical model was constructed to compare the findings with current published data. The true male to female ratio appears to be 3:4. Eighty per cent of females remain undiagnosed at age 18 which has serious consequences for the mental health of young women.

Type 2 diabetes mellitus may be complicated with chronic complications including diabetic neuropathy (DN). Timely diagnosis is crucial in treatment and maybe reversal of this complication. C reactive protein to albumin ratio (CAR) is considered as a marker of inflammation in various diseases that characterized with acute or chronic inflammation. Since diabetic neuropathy and type 2 diabetes mellitus are also associated with chronic, low grade inflammation, we aimed to study CAR levels of type 2 diabetic subjects with diabetic neuropathy and to compare to those in patients without diabetic neuropathy. Type 2 diabetic patients presented to our institutional outpatient clinics were divided into two according to the presence of DN. Subjects with DN were listed as DN group and others were as non-DN group. Characteristics and laboratory data, including CAR, in DN and non-DN groups were compared. Median CAR of the DN and non-DN groups were 2,19 (0,2-49)% and 0,56 (0,02-5,8)%, respectively (p&lt;0.001). CAR was significantly and positively correlated with age (r=0.15, p&lt;0.001), weight (r=0.19, p=0.01), BMI (r=0.11, p=0.03), waist circumference (r=0.10, p=0.046), fasting plasma glucose (r=0.14, p=0.004), serum creatinine (r=0.25, p&lt;0.001), triglyceride (r=0.17, p&lt;0.001), and LDL-cholesterol (r=0.13, p=0.001) levels. Moreover, CAR was inversely correlated with eGFR levels (r=-0.16, p&lt;0.001). The sensitivity and specificity of CAR (when higher than 1,02%) in predicting diabetic neuropathy were 78% and 73%, respectively (AUC: 0.84, p&lt;0.001, 95% CI: 0,82-0,87). Moreover, high CAR level was an independent risk factor of diabetic neuropathy (p&lt;0.001, OR: 1.34, 95% CI: 0.48-1.62). In conclusion, elevated CAR levels could be considered as a marker of diabetic neuropathy.

Backgroundː Previous studies have shown that both serum creatinine and D-dimer levels were associated with atherosclerotic coronary artery disease (CAD). We aimed to determine whether DCR is associated with coronary Gensini score in patients with ST-elevation myocardial infarction (STEMI). Methodsː 337 STEMI patients with complete D-dimer and creatinine and other necessary information were included in the analysis. According to the values of the DCR, patients were divided into the lower DCR group (DCR ≤ 1.42, n = 173) and the higher DCR group (DFR > 1.42, n = 174), and the differences between the two groups were compared. Multivariate linear and multivariate logistic regression analyses were performed to determine independent predictors of Gensini score. Resultsː High DCR group had higher Gensini score compared with low DCR group (P < 0.05). DCR was positively correlated with Gensini score (r=0.493, P < 0.001). Multiple linear regression analysis showed that Previous MI (r=11.312, P=0.035) and DCR (r=5.129, P<0.001) were independent risk factors associated with Gensini score. Multivariate logistic regression analysis showed that, compared to the group 1, DCR is independent risk factor for Group 2, Group 3, Group 4 (P <0.001). DCR is positively correlated with coronary Gensini score in STEMI patients and can be used as an independent predictor of higher Gensini score. Conclusionsː As a new and useful clinical marker, DCR is positively correlated with coronary Gensini score in STEMI patients and can be used as an independent predictor of higher Gensini score.

Abstract: Systemic inflammatory response syndrome (SIRS) is a clinical diagnosis based on abnormalities in patients' vital signs and white blood cell count. Neutrophil-lymphocyte ratio (NLR) is used as an easily accessible parameter to assess the patient's inflammatory status and stress. There are some studies showing that NLR affected by the inflammatory response is a criterion to support the diagnosis of SIRS. In this study, it was aimed to compare the interrelation between SIRS (+) and SIRS (-) groups in patients with high positive feline coronavirus (FCoV) in the light of NLR. Based on the anamneses, physical examination findings, laboratory findings, enzyme immunoassay antibody test kit titer were included in the sick groups. These patients were further categorized based on the presence of SIRS symptoms, vital signs and laboratory findings. The NLR as diagnostic marker demonstrated to differ between high antibody titer FCoV positive and healthy cats. However the diagnostic significance of NLR remains questionable between the high antibody titer FCoV positive cats with SIRS (+) and SIRS (-). As a result of our study NLR can be used as a blood parameter like albumin/globulin ratio and elevated total bilirubin in cats with high FCoV antibody titer and high probability of developing FIP.

The phenomenon of interference is a characteristic of all waves. This paper reviews the interference of classical waves and of matter waves in quantum mechanics. In quantum mechanics, there are two interference mechanisms between identical particles. One is that the de Broglie wavelengths of two interference waves are the same, which is called the equal wavelength interference mechanism. The other is that the wavelengths of interference waves can also be different, in which case there should be an exchange term in additional to the direction term in the wave function. There is a fixed phase difference between the two terms, which causes interference. This is called exchange effect interference mechanism. We propose an experiment, which is simpler to what has been done, to show the exchange effect interference. There is a special case where two interference waves can satisfy both the two mechanisms. We propose an experiment to discriminate that in this case which mechanism plays a role on earth.

One of the most common limits to gas sensor performance is the presence of unwanted interference fringes or etalons arising, for example, from multiple reflections between surfaces in the optical path. Additionally, since the amplitude and the frequency of these interference depend on the distance and alignment of the optical elements, they are affected by temperature changes and mechanical disturbances, giving rise to a drift of the signal. In this work, we present a novel semi-parametric algorithm which allows the extraction of a signal, like the spectroscopic absorption line of a gas molecule, from a background containing arbitrary disturbances, without having to make any assumption on the functional form of these disturbances. The algorithm is applied first to simulated data and then to oxygen absorption measurements in presence of strong fringes.To the best of the authors' knowledge, the algorithm enables an unprecedented accuracy particularly if the fringes have a free spectral range and amplitude comparable to those of the signal to be detected. The described method presents the advantage of being based purely on post processing, and to be of extremely straightforward implementation if the functional form of the Fourier transform of the signal is known. Therefore it has the potential to enable interference-immune absorption spectroscopy. Finally, its relevance goes beyond absorption spectroscopy for gas sensing since it can be applied to any kind of spectroscopic data.

The adsorption of supramolecular composites for Cs(I) in high level liquid waste (HLLW) are different due to different carriers. We chose 25,27-bis(ethyl)-calix[4]arene-26,28-crown-6 (EC[4]C6) as adsorbent, SiO2-P, XAD-7 and UiO-66 as carriers. The synthesis and characterization of supramolecular EC[4]C6 loaded with three different carriers were presented. SEM, N2 adsorption/desorption isotherms, TG-DSC spectra, FT-IR spectra results shown that EC[4]C6 was successfully introduced into the pores of carriers via physical intermolecular interactions. EC[4]C6/SiO2-P, EC[4]C6/XAD-7 and EC[4]C6/UiO-66 shown high efficiency, high selectivity towards Cs(I) over 18 typical fission or corrosion products in HNO3 solution. The adsorption properties of the three materials were investigated after electron irradiation. These results demonstrate that EC[4]C6/SiO2-P possess greater potential for highly efficient removal of Cs(I) from HLLW.

Background: Feline infectious peritonitis (FIP) is a fatal feline disease classified as either effusive (‘wet’), non-effusive (‘dry’), or a mixture of both forms (‘mixed’). The anti-FIP therapeutic effect of Mutian and molnupiravir, two drugs whose active ingredient is a nucleic acid analog, has been confirmed recently. Methods: Of the cats with FIP, we observed a total of 122 and 56 cases achieved remission after administration of Mutian and molnupiravir as routine treatments, respectively. Changes in clinical indicators suggested to be correlated with FIP remission (weight, hematocrit, and albumin to globulin ratio) before and after the administration of each drug and during follow-up observation were statistically compared for each FIP type. Results: In all three FIP types, administration of either Mutian or molnupiravir resulted in statistically significant increases in these indicators. Furthermore, the effect of Mutian on improving the albumin to globulin ratio was not observed at all in wet FIP, as compared with that of molnupiravir, but statistically significant in mixed and dry (P<0.02 and P<0.003, respectively). These clinical features were all due to differences in fluctuations of circulating globulin levels. Conclusions: These results indicate slight inflammatory responses might be elicited continuously by residual feline coronavirus non-disappeared through molnupiravir treatments.

Vibrational overtones in deeply bound molecules are sensitive probes for variation of the proton-to-electron mass ratio μ. In nonpolar molecules, these overtones may be driven as two-photon transitions. Here, we present procedures for experiments with O₂⁺, including state-preparation through photoionization, a two-photon probe, and detection. We calculate transition dipole moments between all X²П_g vibrational levels and those of the A²П_u excited electronic state. Using these dipole moments, we calculate two-photon transition rates and AC-Stark-shift systematics for the overtones. We estimate other systematic effects and statistical precision. Two-photon vibrational transitions in O₂⁺ provide multiple routes to improved searches for μ variation.

Europium (Eu)-doped fluorapatite (FA) nanorods has a similar biocompatibility with hydroxyapatite (HA) in terms, attracted much attention as cell imaging biomaterials due to their luminescent property. Here, we will discuss the new feature of europium doped fluorapatite (Eu-FA) nanorods as anticancer drug carrier. Eu-FA nanorods was prepared using a hydrothermal method. The morphology, crystal structure, fluorescence and composition are investigated. The specific crystal structure, enabling an effective loading of drug molecules. Doxorubicin (DOX), used as an anticancer model drug, was shown to be effectively loaded onto the surface of the nanorods. The DOX release was fairly pH-dependent, occurring more rapidly at pH 5.5 than pH 7.4 was observed. The intracellular penetration of the DOX-loaded Eu-FA nanorods (Eu-FA/DOX) can be imaged in situ due to the self-fluorescence property. Treatment of melanoma A375 cells with Eu-FA/DOX elicited a more effective apoptosis rate than direct DOX treatment. Overall, Eu-FA show great promise for tracking and treating tumor, may potentially useful as a multifunctional carrier system to effectively load and sustainably deliver drugs.

Obesity is a troubling public health problem worldwide as it increases risks of respiratory complications, systemic arterial hypertension, type 2 diabetes mellitus, sleep disorders, cardiovascular diseases, and metabolic syndrome (MetS). As a measure to counteract comorbidities associated with severe obesity, bariatric surgery stands out. This study aimed to investigate the adiponectin/leptin ratio in women with severe obesity with and without MetS undergone Roux-en-Y Gastric Bypass (RYGB) and to characterize the biochemical, glucose, and inflammatory parameters of blood in women with severe obesity before and after RYGB. Were enrolled female with severe obesity undergoing RYGP with MetS (n=11) and without (n=39). Anthropometric data and blood samples were obtained before and 6 months after RYGB to analyze total cholesterol, low-density lipoprotein (LDL), high- density lipoprotein (HDL), non-HDL total cholesterol, and glucose; and circulating levels of adiponectin and leptin. Significant reductions in weight, body mass index, and glucose, total cholesterol, LDL, and leptin were observed after surgery, with higher levels of HDL, adiponectin, and adiponectin/leptin ratio observed after surgery compared to preoperative values. This study demonstrated that weight loss induced by RYGB in patients with severe obesity with or without MetS improved biochemical and systemic inflammatory parameters, particularly the adiponectin/leptin ratio.

Preconception or prenatal carrier screening for hereditary deafness is an effective early intervention strategy to reduce the incidence of newborn deafness, but current research is limited. This study aimed to investigate the carrier frequencies of common deafness genes in the Chinese population and to follow up on pregnancy outcomes in high-risk couples. Carrier screening for common deafness genes in the Chinese population, including the GJB2 and SLC26A4 genes, was performed using next-generation sequencing technology. Of the 9,993 subjects screened, the carrier rate was 2.86% for the GJB2 gene and 2.63% for the SLC26A4 gene. Of the six high-risk couples, four made alternative reproductive decisions (three with prenatal diagnosis and one with preimplantation genetic testing), with consequent successful termination of the birth of two affected fetuses. The frequencies of preconception or prenatal carrier screening for the deafness genes GJB2 and SLC26A4 in 9,993 subjects from China in this study were 2.86% and 2.63%, respectively. In addition, four out of six high-risk couples made alternative reproductive decisions, followed by the successful prevention of the birth of two affected fetuses. These findings confirmed the clinical utility of preconception or prenatal carrier screening for hereditary deafness.

The study evaluated potassium peroxymonosulfate (PPMS) efficacy against rifampicin-resistant Salmonella Infantis (rif-SI), Escherichia coli (E. coli), Salmonella Infantis (SI), Newcastle disease virus (NDV) and avian influenza virus (AIV). The assessment encompassed varied concentrations, organic material conditions, and exposure durations to comprehensively evaluate PPMS efficacy. Moreover, PPMS’s efficacy was tested on diverse carrier surfaces and clothing to evaluate its bactericidal and virucidal potential. At the recommended concentrations (1X), PPMS effectively inactivated all pathogens within 5 sec, except for NDV, which required 30 sec in the presence of organic materials. At 0.5X, PPMS successfully inactivated E. coli, AIV and NDV within 5 sec in the absence of organic material. However, rif-SI and SI necessitated 30 sec for inactivation. Most pathogens were inactivated within 30 sec, with the exception for AIV, which was inactivated within 5 sec. PPMS effectively eliminated rif-SI on all carriers within 30 sec. Moreover, at both concentrations, PPMS successfully inactivated AIV on all carriers within 30 sec, except on stainless steel at 0.5X. Furthermore, PPMS at both concentrations required 5 and 30 sec, respectively, to inactivate AIV on the rayon sheet. Therefore, due to its demonstrated efficacy against a wide range of pathogens across diverse surfaces, PPMS emerges as a crucial alternative disinfectant significantly enhancing biosecurity on and around chicken farms. found in and around animal farms, slaughterhouses, and hospitals.

Advanced derivatives of the CXCR4 antagonist EPI-X4 have shown therapeutic efficacy upon topical administration in animal models of asthma and dermatitis. Here, we studied plasma stability of the EPI-X4 lead compounds WSC02 and JM#21, using mass spectrometry to monitor the chemical integrity of the peptides and a functional fluorescence-based assay that determines peptide function in a CXCR4-antibody competition assay. Although mass spectrometry revealed a very rapid disappearance of both peptides in human plasma within seconds, the functional assay revealed a significantly higher half-life of 9 minutes for WSC02 and 6 minutes for JM#21, respectively. Further analyses demonstrated that WSC02 and JM#21 interact with low molecular weight plasma components and serum albumin. Albumin binding is mediated by the formation of a disulfide bridge between Cys10 in the EPI-X4 peptides and Cys34 in albumin. These covalently linked albumin-peptide complexes have a higher stability in plasma as compared to the non-bound peptides and retain the ability to bind and antagonize CXCR4. Remarkably, chemically synthesized albumin-EPI-X4 conjugates coupled by non-breakable bonds have a drastically increased plasma stability of over 2 hours. Thus, covalent coupling of EPI-X4 to albumin in vitro before administration or in vivo post administration may significantly increase the pharmacokinetic properties of the new class of CXCR4 antagonists.

Mitochondrial dysfunction and oxidative stress are thought to be relevant to the pathogenesis of age-related macular degeneration (AMD). Glutathione (GSH) homeostasis fulfills a number of important roles in mitochondria, such as maintenance of mitochondrial DNA and respiratory competency of cells. Although the transport of mitochondrial GSH (mGSH) is not fully understood, increasing evidence from non-ocular tissues suggests that OGC (2-oxoglutarate carrier, SLC25A11) and DIC (dicarboxylate carrier, SLC25A10) are involved in mGSH transport. However, whether OGC and DIC mediate the transfer of GSH into the mitochondria of retinal pigment epithelial cells (RPE) remains unknown. Thus, we investigated the expression, localization, and function of OGC and DIC in human RPE (hRPE) in relation to oxidative stress and GSH. Both OGC and DIC are expressed in hRPE and are localized in mitochondria. We also found a dose and time-dependent decrease of OGC and DIC expression under oxidative stress and increased expression in polarized RPE. Our data show that the downregulation of OGC and DIC resulted in increased apoptosis and mGSH depletion which can be overcome by co-treatment with GSH-MEE. These findings suggest that overexpression of OGC and DIC may be an effective strategy to decrease susceptibility to mitochondrial toxicants by elevation of mGSH.

We experimentally study the supercontinuum (SC) induced by femtosecond filament in different liquid media, and determine the relative measurement value of filamentation threshold Pth for these media by using a Mach-Zehnder interferometer (MZI). It is found that the value of Pth is higher than the critical power for self-focusing Pcr, which can be attributed to the strong dispersion effect. Changing the focal length of the focusing lens affect the measured results of the filamentation threshold: In the case of shorter focal length, linear focusing (i.e., geometrical focusing) regime dominates, and the measured values of Pth for different media are almost the same; as the focal length becomes larger, self-focusing starts to play a role, making the values of Pth for different media different from each other. This work provides an effective approach to study the femtosecond filamentation process in liquid media, and helpful to further understand the physical process of supercontinuum induced by femtosecond filament in liquid media.

As a fundamental optical approach to interferometry, Sagnac interference has been widely used for reflection manipulation, precision measurements, and spectral engineering in optical systems. Compared to other interferometry configurations, it offers attractive advantages by yielding a reduced system complexity without the need for phase control between different pathways, thus offering a high degree of stability against external disturbance and a low wavelength dependence. The advance of integration fabrication techniques has enabled chip-scale Sagnac interferometers with greatly reduced footprint and improved scalability compared to more conventional approaches implemented by spatial light or optical fiber devices. This facilitates a variety of integrated photonic devices with bidirectional light propagation, showing new features and capabilities compared to unidirectional-light-propagation devices such as Mach-Zehnder interferometers (MZIs) and ring resonators (RRs). Here, we present our latest results for functional integrated photonic devices based on Sagnac interference. We outline the theory of integrated Sagnac interference devices with comparisons to other integrated photonic building blocks such as MZIs, RRs, photonic crystal cavities, and Bragg gratings. We present our latest results for Sagnac interference devices realized in integrated photonic chips, including reflection mirrors, optical gyroscopes, basic filters, wavelength (de)interleavers, and optical analogues of quantum physics.

During the launch and return of a spacecraft, the intense combustion of propellants generates strong electromagnetic radiation, which interferes with the operation of electronic equipment in the spacecraft. To improve the electromagnetic compatibility of electronic equipment in spacecraft, it is necessary to study the electromagnetic radiation characteristics of rocket fuel. An electromagnetic radiation measurement system based on antennas is designed to measure the electromagnetic radiation generated by rocket fuel, and the electromagnetic radiation characteristics are obtained through data analysis. The mechanism of the electromagnetic radiation generated by rocket fuel is comprehensively analysed through the spatial, time-domain, frequency-domain, and energy-domain characteristics. A characterization model is established to provide a reliable scheme for evaluating the influence of rocket fuel electromagnetic radiation on electronic equipment in spacecraft.

Background and objectives: The fact that the results of troponin and Nt-proBNP interfere from biotin caused some commercial firms to update their measurement methods. In particular, the clinical incompatibility of cardiac test results may affect the risk of morbidity and mortality. The aim of this study is to investigate the interference effects of 7 different contrast agents on cardiac markers (Troponin-I, Nt-proBNP, Mass CK-MB, CK, AST, LDH) and coagulation tests (PT, APTT). Materials and Methods: Seven different contrast medias were added into control materials by using interference protocol. Concentration of PT, APTT, CK, AST, LDH, Mass CK-MB, Troponin-I, Nt-proBNP were measured by Sysmex CS-2100, Abbott c16000, Siemens Centaur XP and AFİAS-6 analyzer. The amount of deviations from target values were calculated. Results: 7 different contrast medias caused negative interference in troponin levels between 57.43% and 62.87%. It was found that different contrast medias produced false negativity in the Nt-proBNP test ranging from 6.11% to 96.01%. Enzymes and coagulation tests have been less affected. Conclusions: Different contrast medias may cause false negative cTnI and Nt-proBNP. Therefore, the contrast medias which causes the least interference should be preferred. The results of samples taken in the first hour of contrast imaging should be interpreted with care.

Background: Immunosuppressant blood levels should be measured at regular periods in order to keep them within the therapeutic index. Although LC-MS/MS is preferred as a reliable method, some molecules like radiopaque agents in blood matrix may lead to false results. The aim of this study is to investigate the effect of seven different radiopaque agents on immunosuppressant drugs. Methods: Seven different radiopaque agents were added into control materials containing tacrolimus, everolimus, sirolimus and cyclosporine A drugs. Measurements were performed by LC-MS/MS instrument. The amount of deviations from target values were calculated. Results: Immunosuppressant blood levels significantly changed after the administration of radiopaque agents. Seven different radiopaque products led to false negative results in tacrolimus and cyclosporine A levels at a rate of 19.77% to 44.45%. The smallest deviations were seen in everolimus levels with administration of RM6 (gadodiamide) and in sirolimus levels with RM1 (gadobutrol) at rates of 4.04% and 2.11%, respectively. The highest deviations were observed with RM3 (iohexol) administration in everolimus and sirolimus levels at rates of 153.72% and 171.41%, respectively. Conclusions: False immunosuppressant results associated with radiopaque agents may result in organ rejection. Preferring radiopaque agents that cause the least interference risk is important to reduce the organ rejection risk. However, the least risky method is to obtain samples for drug levels before contrast-enhanced imaging.

This study explores individual and contextual risk factors for the onset of work interfering with private life (WIL) and private life interfering with work (LIW) among self-employed men and women across European countries. It also studies the relationship between interference (LIW and WIL) and wellbeing among self-employed men and women and the effect of macro level risk factors. Data from the fifth round of European Working Conditions Survey was utilized and a sample of self-employed men and women with active businesses was extracted. Applying multilevel regressions, results show that though business characteristics are important for level of WIL, time demand is the most evident risk factor for WIL and LIW. There is a relationship between wellbeing and WIL and LIW respectively, and time demands is the most important factor in this relationship. Gender equality on the labor market did not relate to level of interference, nor did it mediate the relationship between interference and wellbeing. However, the main and most important risk factor for experiencing WIL and LIW and for how interference relate to wellbeing is gender relation processes in work and life, both on individual and contextual level.

In this paper, we have proposed an effective metasurface design to accomplish cloaking of equilateral patch antennas and their array configuration. As such, we have exploited the concept of electromagnetic invisibility, employing the mantle cloaking technique with the intention to eliminate the destructive interference ensuing between two distinct triangular patches situated in a very congested arrangement (sub-wavelength separation is maintained between the patch elements). Basically, we demonstrate that implementation of the planar coated metasurface cloaks onto the patch antenna surfaces compels them to become invisible to each other, at the intended frequencies. In effect, an individual antenna element does not sense the presence of the other, in spite of being in a rather close vicinity. We also exhibit that the cloaks successfully reinstate the radiation attributes of each antenna in such a way that it emulates their respective performance in an isolated environment. Moreover, we have extended the cloak design to an interleaved one dimensional array of the two patch antennas and it is shown that the coated metasurfaces assure efficient performance of each array in terms of their matching as well as radiation characteristics, in turn, enabling them to radiate independently for various beam-scanning angles.

With the rapid population growth, there is an urgent need for innovative crop improvement approaches to meet the increasing demand for food. Classical crop improvement approaches involve, however, a backbreaking process that cannot equipoise with increasing crop demand. RNA based approaches i.e. RNAi-mediated gene regulation and site-specific nuclease based CRISPR/Cas9 system for gene editing has made advances in the efficient targeted modification in many crops for the higher yield and resistance to diseases and different stresses. In functional genomics, RNA interference (RNAi) is a propitious gene regulatory approach that plays a significant role in crop improvement by permitting down-regulation of gene expression by small molecules of interfering RNA without affecting the expression of other genes. Gene editing technologies viz. clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) have appeared prominently as a powerful tool for precise targeted modification of nearly all crops genome sequence to generate variation and accelerate breeding efforts. In this regard, the review highlights the diverse roles and applications of RNAi and CRISPR/Cas9 system as powerful technologies to improve agronomically important plants to enhance crop yields and increase tolerance to environmental stress (biotic or abiotic). Ultimately, these technologies can prove to be important in view of global food security and sustainable agriculture.

Tumor immunotherapy represented by immune target blocking and chimeric antigen receptor cell therapy has developed rapidly. Among them, Natural Killer Cell (NK) is gradually becoming another preferred immunotherapy method after T cell immunotherapy because of its unique killing effect in innate immunity and adaptive immunity. Although NK cell therapy has achieved promising results in clinical trials, there are still some problems, such as low efficacy in solid tumors, insufficient penetration of NK cells and high cost of treatment. Nanomaterials are expected to be an excellent tool to improve the anti-tumor immune response of NK cells due to their advantages such as chemical specificity, biocompatibility and simple synthesis. Nanomaterials can enhance the cytotoxicity of NK cells, participate in the modification and noninvasive cell-tracking patterns of NK cells，and mediate their homing and infiltration, and thus significantly improve the efficiency of NK cell immunotherapy. In this review, we will briefly introduce the methods and mechanisms of tumor immunotherapy, emphasize the research progress of NK cell-based immunotherapy and imaging, and finally summarize the direction and research progress of nanomaterials in NK cell-based immunotherapy.

Cyanobacteria are promising organisms for the sustainable production of various biotechnologi-cal interesting products. Due to their energy production via photosynthesis, the cultivation of cyanobacteria expands the CO2 cycle. Most cyanobacteria form biofilms on surfaces in their natu-ral environment by surrounding the cells with a self-produced matrix of extracellular polymeric substances (EPS) that hold the cells together. These special growth properties need special reac-tors for cultivation. By immobilizing cyanobacteria on carriers, systems currently established in industry could also be used for biofilm formers. Various artificial carriers for immobilized growth of cyanobacteria and microalgae have already been described in the literature. However, the use of waste materials or natural biodegradable carriers would be more sustainable and is, therefore, the focus of this study. Dried Luffa cylindrica, zeolite, and corn stalks were investigated for their use as carriers for cyanobacteria. L. cylindrica was shown to be an excellent natural carrier for (i) Anabaena cylindrica (ii) Nostoc muscorum 1453-12a, and (III) Nostoc muscorum 1453-12b. Higher or at least similar growth rates were achieved when cyanobacteria were cultivated with L. cylindrica compared to submerged cultivation. Additionally, the production of EPS and C-phycocyanin was increased at least 1.4-fold in all strains by culturing on L. cylindrica. The improved growth could be explained on the one hand by the high surface area of L. cylindrica and its properties, and on the other hand by the release of growth-promoting nutrients from L. cylindrica to the medium.

The presence of high concentrations of flammable gases and volatile organic compounds in the atmosphere has been widely reported to be detrimental to human survival. A lot of research effort has been put towards finding an efficient means of quick detection of these gases below their ‘immediately dangerous to life or health’ concentrations. Detecting these gases in an oxygen-deficient environment is a crucial task to consider and has been overlooked. In this research, double substitution spinel with chemical formula Co1-2xNixMnxFe2-yCeyO4, where 0 ≤ x = y ≤ 0.3, were prepared via the glycol-thermal technique. The final products following the appropriate substitution are CoFe2O4 (dried naturally), CoFe2O4 (dried with Infrared lamp), Co0.8Ni0.1Mn0.1Fe1.9Ce0.1O4, Co0.6Ni0.2Mn0.2Fe1.8Ce0.2O4, and Co0.4Ni0.3Mn0.3Fe1.7Ce0.3O4 spinel ferrites. The X-ray diffractometry (XRD), High resolution transmission electron micrographs (HRTEM), and X-ray photoelectron spectroscopy (XPS) of the samples confirm the formation of the spinel. The gas sensing performance of this samples were tested at operating temperature of 225 ºC towards liquefied petroleum gas (LPG), ammonia, ethanol, and propanol. The Co0.8Ni0.1Mn0.1Fe1.9Ce0.1O4-based sensor was selective to LPG, with a high response of 116.43 toward 6 000 ppm of LPG when helium was used as carrier gas, 3.35 when dry-air was the carrier gas, 4.4 when nitrogen was the carrier gas, but was not sensitive when argon was used as a carrier gas.

Current sources play an essential role in tissue excitation used in bioelectrical impedance spectroscopy. Most investigations use Howland current sources that, despite their practicality and simplified implementation, have operating frequency limitations and dependence on the load impedance due to theirs narrow output impedance, specially at higher frequencies. The objective of this work is to propose model for a robust current-controlled sinusoidal oscillator. The oscillator is based on fully analog electronics, which enables controlling oscillation phase and amplitude by using a voltage reference. The mathematical model is based on Pyragas control application to the classical harmonic oscillator. From the modelling process, it was build an oscillator topology based on second-generation current carriers and on transconductance amplifiers. The reference signal (Fsync) was a sinusoidal voltage source having a frequency of 1MHz and an amplitude of 1Vpp. The oscillator output current synchronized the oscillations’ phase and amplitude with Fsync, regardless of their magnitude before the control signal acted in the circuit at t≈13.5μs. SPICE simulations using ideal components have confirmed the successful operation of the proposed oscillator. This type of oscillator can be implemented in SOIC, then allowing oscillation control interface with logic circuits.

InGaN/GaN samples grown on c-plane sapphire substrate with different In concentrations by metal organic chemical vapor deposition are demonstrated. The subsequent capping GaN layer growth opens a possibility for dislocation reduction due to the lateral strain relaxation in growth geometry. We present the further growth optimization and innovative characterization of InGaN layers overgrown on different structures with varying In concentrations. The photoelectrical and optical properties of the InGaN layers with or without capping GaN layer were investigated by time-resolved picosecond transient grating and temperature dependence photoluminescence. We note a 10-fold increase in carrier lifetime in the InGaN layers when the sample structure changes from PIN to single InGaN layer.

Shielding agents are commonly used to shield polyelectrolyte complexes, e.g. polyplexes, from agglomeration, precipitation in complex media, like blood, and thus enhance their circulation times in vivo. Since up to now primarily poly(ethylene glycol) (PEG) has been investigated to shield non-viral carriers for systemic delivery, we report on the use of polysarcosine (pSar) as a potential alternative for steric stabilization. A redox-sensitive, cationizable lipo-oligomer structure (containing two cholanic acids attached via a bioreducible disulfide linker to an oligoaminoamide backbone in T-shape configuration) was equipped with azide-functionality by solid phase supported synthesis. After mixing with small interfering RNA (siRNA), lipopolyplexes formed spontaneously and were further surface-functionalized with polysarcosines. Polysarcosine was synthesized by living controlled ring-opening polymerization using an azide-reactive dibenzo-aza-cyclooctyne-amine as an initiator. The shielding ability of the resulting formulations was investigated with biophysical assays and by near-infrared fluorescence bioimaging in mice. The modification of ~100 nm lipopolyplexes was only slightly increased upon functionalization. Cellular uptake into cells was strongly reduced by the pSar shielding. Moreover, polysarcosine-shielded polyplexes showed enhanced blood circulation times in bioimaging studies compared to unshielded polyplexes and similar to PEG-shielded polyplexes. Therefore, polysarcosine is a promising alternative for the shielding of non-viral, lipo-cationic polyplexes.

Text-based CAPTCHA remains the most widely adopted security scheme, which is the first barrier to securing websites. Deep learning methods, especially Convolutional Neural Networks (CNNs) are the mainstream approach for text-CAPTCHA recognition, which are widely used in CAPTCHA vulnerability assessment and data collection. However, verification code recognizers are mostly deployed on the CPU platform as part of a web crawler and security assessment, they are required to have both low complexity and high recognition accuracy. Due to the specifically designed anti-attack mechanisms like noise, interference, geometric deformation, twisting, rotation, and character adhesion in text CAPTCHAs, some characters are difficult to efficiently identify with high accuracy in these complex CAPTCHA images. This paper proposed a recognition model named Adaptive-CAPTCHA with a CRNN module and trainable and configurable filtering networks, which effectively handle the interference and learn the correlation between characters in CAPTCHAs to enhance recognition accuracy. Experimental results on two datasets of different complexity show that compared with the baseline model Deep-CAPTCHA, the number of parameters of our proposed model is reduced by about 70% and the recognition accuracy is improved by more than 10 percentage points in the two datasets. In addition, the proposed model has a faster training convergence speed.

The use of antibiotics grown promoter has been banned, due led problems with drug residues in animal products and increased bacterial resistance. For several reasons there is a growing interest in the scientific community in immunoglobulin Y as antibiotic alternative and their oral administration in the polyclonal antibody (pAb) format, to maintain animal health and performance, do not require IgY purification for large-scale production, resulting in protein impurities and high concentration of biotin in the samples. The signal amplification through non-covalent interaction of biotin with streptavidin has been extensionally used by many laboratories for several diagnosis diseases and scientific research in enzyme-linked immunosorbent assay (ELISA). However, the riches sources of biotin as egg yolk, leading to high biotin concentrations in samples, harming the accuracy of diagnostic and proteins concentrations tests. This study aimed to evaluate the biotin influence on measurement of Immunoglobulin Y in egg yolk freeze dried samples from immunized laying hens by immunoassays using biotin-avidin/streptavidin. The IgY concentration changed down in immunoassays using biotin-avidin/streptavidin. The detection of IgY in yolk samples by ELISA using streptavidin–biotin binding as part of the assay methodology requires some technology to neutralize high concentration of biotin on sample or more steps beyond delipidation to isolate the target protein. Otherwise, an ELISA without the use of streptavidin-biotin binding would be more advisable to avoid biotin and targets protein relationship and prevent biotin interference on results.

Quantum physics through the lenses of Bayesian statistics consider probabilities to be degree of believes or knowledge and subjective. A Bayesian derivation of the probability density function in phase space is presented. The entropy proposed by Geiger and Kedem \cite{GeiKed2022a,GeiKed2022b} in phase space is expanded to define quantitatively interference and entanglement. Comparisons between each of these two quantities and the entropy are made. A brief presentation of entanglement in phase space to the spin degree of freedom and to mixed states completes the work.

Bobbin Tool Friction Stir Welding (BTFSW) is a variant of FSW process using the special two shoulder tool that forms the top and bottom weld surface. As such, a significant simplification of welding setup is achieved. One of the dominant parameters of BTFSW process is the interference between tool shoulder pinch gap and weld metal thickness. In this research, the influence of interference of the square pin tool with convex shoulders on process temperature, microstructure, tensile, impact and bend performance were studied, and appropriate correlations were devised. The base metal was aluminum-magnesium alloy, with the interference varied in the range of 0.1 to 0.5 mm. The worm defects and irregularities were found in all specimens except in specimen welded with 0.4 mm interference. Optimal interference of 0.4 mm resulted in the highest mechanical properties, which, in terms of tensile strength and reduction of area were similar to the base metal. Furthermore, impact strength was significantly higher, which was attributed to grain refinement effect in the nugget zone.

In this paper, a range-gated lidar system utilizing an LN crystal as the electro-optical switch and a SCMOS (Scientific Complementary Metal Oxide Semiconductor) imaging device is designed. To achieve range-gated, we utilize two polarizers and a LN (LiNbO3) crystal to form an electro-optical switch. The optical switch is realized by applying a pulse voltage at both ends of the crystal due to the crystal's conoscopic interference effect and electro-optical effect. The advantage of this system is that low-bandwidth detectors such as CMOS and CCD (Charge-coupled Device) can be used to replace conventional high-bandwidth detectors such as ICCD (Intensified Charge Coupled Device), and time it has better imaging performance under specific conditions at the same. However, after using an electro-optical crystal as an optical switch, a new inhomogeneity error will be introduced due to the conscopic interference effect of the electro-optical crystal, resulting in range error of the lidar system. To reduce the influence of inhomogeneity error on the system, this paper analyzes the sources of inhomogeneity error caused by the electro-optical crystal and gives the crystal inhomo-geneity mathematical expression. A compensation method is proposed based on the above inho-mogeneity mathematical expression. An experimental lidar system is constructed in this paper to verify the validity of the compensation method. The experimental results of the range-gated lidar system show that in a specific field of view (2.6mrad), the lidar system has a good imaging per-formance, its ranging standard deviation is 3.86cm and further decreased to 2.86cm after com-pensation, which verifies the accuracy of the compensation method.

Molecular communication (MC) is a promising bio-inspired paradigm for exchanging molecule information among nanomachines. This paper proposes a synchronisation-assist photolysis MC system that aims to transmit the bio-sensing signal of the tumour microenvironment, facilitated by mitigating redundant molecules for improved bit error rate (BER) performance. Benefits from bio-compatible MC, biosensors could transmit bio-sensing signals of the tumour in $vivo$ instead of converting them to electrical signals. Due to diffusion motion's slow and stochastic nature, inter-symbol interference (ISI), resulting from previous symbols' residual information molecules, inevitably occurs in diffusion-based MC. ISI is one of the challenges in diffusion-based MC, which significantly impacts signal detection. Inspired by on-off keying (OOK) modulation, the proposed modulation implements a switch of molecules and light alternatively. The light emitted is triggered by a synchronisation signal, and the photolysis reactions could reduce the redundant molecules. An expression for the relevant channel impulse response (CIR) is derived from a hybrid channel model of diffusion and photolysis-reaction. This paper implements the maximum posterior estimation scheme to find the optimal decision threshold and analysis the BER performance in terms of different time intervals of the system. Numerical simulations demonstrate that the proposed method can improve the channel capacity and BER performance. We believe that our work may pave the way for MC application in bio-sensing.

For the elimination of radio-frequency interference (RFI) in a passive microwave radiometer, the threshold level is generally calculated from the mean value and standard deviation. However, a serious problem that can arise is an error in the retrieved brightness temperature from a higher threshold level owing to the presence of RFI. In this paper, we propose a method to detect and mitigate RFI contamination using the threshold level from statistical criteria based on a spectrogram technique. Mean and skewness spectrograms are created from a brightness temperature spectrogram by shifting the 2-D window to discriminate the form of the symmetric distribution as a natural thermal emission signal. From the remaining bins of the mean spectrogram eliminated by RFI-flagged bins in the skewness spectrogram for data captured at 0.1-s intervals, two distribution sides are identically created from the left side of the distribution by changing the standard position of the distribution. Simultaneously, kurtosis calculations from these bins for each symmetric distribution are repeatedly performed to determine the retrieved brightness temperature corresponding to the closest kurtosis value of three. The performance is evaluated using experimental data, and the error in the retrieved brightness temperature is observed to be less than approximately 3 K from a window with a size of 100 × 100 time-frequency bins according to the RFI levels and cases.

Optical modes bearing optical vortices are important light systems in which to encode information. Optical vortices are robust features of optical beams that do not dissipate upon propagation. Thus decoding the modal content of a beam is a vital component of the process. In this work we present a method to decode modal superpositions of light beams that contain optical vortices. We do so using shear interferometry, which presents a simple and effective means of determining the vortex content of a beam, and extract the parameters of the component vortex modes that constitute them. We find that optical modes in a beam are easily determined. Its modal content can be extracted when they are of comparable magnitude. The use of modes of well defined topological charge but not well defined radial-mode content, such as those produced by phase-only encoding, are much easier to diagnose than pure Laguerre-Gauss modes.

Cable interference in cable-driven mechanisms (CDM) is a major challenge in their applications. The wrench closure workspace (WCW) in a fully constrained mechanism may not be realized unless cable interference is considered. This paper classifies all possible types of cable interferences for planar cable-driven mechanisms and presents an analytical solution to find the boundaries of the constant-orientation interference region. It is shown that interference between cables starts and ends when one end of a cable coincides with another cable which can be thought as lying a point on a line segment. This geometrical view is developed and applied to provide a framework for the analysis of cable interference. By subtracting the interference space form WCW, the interference-free workspace is obtained in which the robot is able to operate.

Pulverized coal pyrolysis based on solid heat carrier has a huge advantage in high tar yield. In this study, pyrolysis experiments of pulverized coal were conducted in a lab-scale fluidized bed reactor at 650 ℃, utilizing char as the solid heat carrier. The influence of mass ratio of char to coal (RATIO) was investigated. Results show that the incorporation of solid heat carrier of char significantly enhanced the initial reaction in coal pyrolysis, resulting in increasing yields of tar and gas but reducing one of char. The yield of tar maximally reached 148.80-262.22% of the Gray-King analysis value at the RATIO of 14.52 g/g. As the RATIO increased, the tar contained more light component content, indicating that incorporating solid heat carriers improved the tar quality. These findings offer significant insights for the design of fluidized bed pyrolysis unit utilizing char as solid heat carrier.

Recently, it was proposed that the thiophene ring is capable of promoting mitochondrial accumulation when linked to fluorescent markers. As a noncharged group, thiophene presents several advantages from a synthetic point of view, making it easier to incorporate such a side moiety into different molecules. Herein, we confirm the general applicability of thiophene group as mitochondrial carrier for drugs and fluorescent markers, based on a new concept of nonprotonable, noncharged transporters. We implemented this concept in a medicinal chemistry application by developing an anti-tumoral, metabolic chimeric drug, based on PDHK inhibitor dichloroacetate (DCA). The promising features of the thiophene moiety as a noncharged carrier for targeting mitochondria may represent a starting point for the design of new metabolism-aimed drugs.

This paper investigates the influence of magnetic field for a two dimensional deformations on a two temperature problem at the free surface of a semi-infinite semiconducting medium under the effect of mechanical force during a photothermal theory and the effect of hydrostatic initial stress on the medium. The Harmonic Wave Method (Normal Mode Analysis) has been used to obtain the equations of elastic waves, heat conduction equation, quasi-static electric field, carrier density, two temperature coefficient, ratios, and constitutive relationships for the thermo-magnetic-electric medium. The effects of several parameters as thermoelastic and thermoelectric coupling parameters and two temperature parameter of this force on the displacement component, force stress, carrier density and temperature distribution has been depicted graphically.

The engagement of the arresting hook with the arresting cable is a critical maneuver that is essential to the safe operation of aircraft landing on aircraft carrier. A comprehensive understanding of the engagement process dynamics is necessary to optimize landing performance and ensure the safety and efficiency of carrier operations. In this paper, an efficient and accurate simulation and analysis method is presented for studying the arresting hook engaging arresting cable process. The Finite Element Method and Multibody Dynamics (FEM-MBD) approach is employed. By establishing a rigid-flexible coupling model encompassing the aircraft frame, arresting hook, carrier deck and arresting gear system, the dynamic model for the engagement process is obtained. The model incorporates multiple coordinate systems to effectively capture the relative motion between the rigid and flexible components, enabling a thorough understanding of the dynamics characteristics. The analysis conducted in this paper takes into account various factors, including the material properties of the components, the characteristics of the arresting gear system, and the state of the aircraft during the engagement process. The analysis method is verified by comparing the simulation results with experiments of arresting hook rebound obtained from reference. Finally, simulations are performed to analyze the engagement process under different touchdown points and rolling angle of aircraft. The simulation results provide valuable insights into the distribution of stresses during the arresting hook and cable engagement, the center of gravity variations, as well as the response of the tire touch and rollover cable under specific scenarios. The proposed rigid-flexible coupling arresting dynamics model in this paper enables effective analysis of the dynamic behavior during arresting hook engaging arresting cable. The results obtained from this analysis offer valuable insights into the performance of the engagement process, which can be used to improve the design of carrier-based aircraft and techniques for carrier landing.

The emergence of pathogenic fungi is a major and rapidly growing problem (7% increase) that affects human and animal health, ecosystems, food security and the economy worldwide. The Dermocystida group in particular has emerged relatively recently and includes species that affect both humans and animals. Within this group, one species in particular, Sphareothecum destruens, also known as the Rosette agent, represents a major risk to global aquatic biodiversity and aquaculture and has caused severe declines in wild fish populations in Europe and large losses in salmon farms in the USA. It is a species that has been associated with a healthy carrier for millions of years but the host has managed in recent decades to invade parts of Southeast Asia, Central Asia, Europe and North Africa. In order to better understand the emergence of this new disease, we have for the first time synthesised current knowledge on the distribution, detection, prevalence of S. destruens as well as the associated mortality curves, and the potential economic impact in countries where the healthy carrier has been introduced. Finally, we propose solutions and perspectives to manage and mitigate the emergence of this fungus in countries where it has been introduced.

Transmembrane transport of small organic and inorganic molecules is one of the cornerstones of cellular metabolism. Among transmembrane transporters, solute carrier (SLC) proteins form the largest, albeit very diverse, superfamily with over 400 members. It has been early on recognized that xenobiotics can directly interact with SLCs and that this interaction can fundamentally determine their efficacy, including bioavailability and intertissue distribution. Apart from the well-established prodrug strategy, the chemical ligation of transporter substrates to nanoparticles of various chemical composition has recently been used as a means to enhance their targeting or absorption. In this review, we summarize efforts in drug design exploiting interactions with specific SLC transporters to optimize their therapeutic effects. Furthermore, we describe current and future challenges as well as new directions for the advanced development of therapeutics that target SLC transporters.

As one of the representatives of emerging metallic transition metal dichalcogenides, niobium ditelluride (NbTe₂) has attracted intensive interest recently due to its distorted lattice structure and unique physical properties. Here, we report on the ultrafast carrier dynamics in NbTe₂ measured using time-resolved pump-probe transient reflection spectroscopy. A thickness-dependent carrier relaxation time is observed, exhibiting a clear increase in the fast and slow carrier decay rates for thin NbTe₂ flakes. In addition, pump power dependent measurements indicate that the carrier relaxation rates are power-independent, with the peak amplitude of the transient reflectivity increasing linearly with pump power. Isotropic relaxation dynamics in NbTe₂ is also verified by performing polarization-resolved pump-probe measurements. These results provide an insight into the light-matter interactions and charge carrier dynamics in NbTe₂ and will pave the way for its applications to photonic and optoelectronic devices.

Purpose: Expanded carrier screening (ECS) informs couples of their risk of having offspring affected by certain genetic conditions. Limited data exists assessing the actions and reproductive outcomes of at-risk couples (ARCs). We describe the impact of ECS on planned and actual pregnancy management in the largest sample of ARCs studied to date. Methods: Couples who elected ECS and were found to be at high risk of having a pregnancy affected by at least one of 176 genetic conditions were invited to complete a survey about their actions and pregnancy management. Results: Three hundred ninety-one ARCs completed the survey. Among those screened before becoming pregnant, 77% planned or pursued actions to avoid having affected offspring. Among those screened during pregnancy, 37% elected prenatal diagnostic testing (PNDx) for that pregnancy. In subsequent pregnancies that occurred in both the preconception and prenatal screening groups, PNDx was pursued in 29%. The decision to decline PNDx was most frequently based on the fear of procedure-related miscarriage, as well as the belief that termination would not be pursued in the event of a positive diagnosis. Conclusions: ECS results impacted couples’ reproductive decision-making and led to altered pregnancy management that effectively eliminates the risk of having affected offspring.

Landing is one of the most dangerous tasks in all the operations on the aircraft carrier, and the landing safety is very important to the pilot and the flight deck operation. The problem of landing path selection is studied in this paper as there several candidates corresponding to different situations. A fuzzy path selection strategy is proposed to solve the problem considering the fuzziness of environmental information and human judgment, and the goal is to provide the pilot with more reasonable decision. The strategy is based on Fuzzy Multi-attribute Group Decision Making (FMAGDM), which has been widely used in industry. Firstly, the background of the path selection problem is given. Then the essential elements of the problem are abstracted to build the conceptual model. A group decision-making method is applied to denote the preference of each decision maker for each alternative route, and the optimal landing path under the current environment is determined taking into account the knowledge and the weight of both decision makers. Experimental studies under different setups, i.e., different environments, are carried out. The results demonstrate that the proposed path selection strategy is validated in different environments, and the optimal landing paths corresponding to different environments can be determined.

Citrullinemia is the earliest identifiable biochemical abnormality in neonates with intrahepatic cholestasis due to a citrin deficiency (NICCD) and it has been included in newborn screening panels using tandem mass spectrometry. However, only one neonate was positive among 600,000 infants born in Sapporo city and Hokkaido, Japan between 2006 and 2017. We investigated 12 neonates with NICCD who were initially considered normal in newborn mass screening (NBS) by tandem mass spectrometry, but were later diagnosed with NICCD by DNA tests. Using their initial NBS data, we examined citrulline concentrations and ratios of citrulline to total amino acids. Although their citrulline values exceeded the mean of the normal neonates and 80 % of them surpassed +3SD, all were below the cutoff of 40 nmol/mL. The ratios of citrulline to total amino acids significantly elevated in patients with NICCD compared to the control. By evaluating two indicators simultaneously, we could select about 80% of patients with missed NICCD. Introducing an estimated index comprising citrulline values and citrulline to total amino acid ratios could assure NICCD detection by NBS.

Passive radar detection emerges as a pivotal method for environmental perception and target de-tection within radar applications. Leveraging its advantages, including minimal electromagnetic pollution and efficient spectrum utilization, passive radar methodologies have garnered increasing interest. In recent years, there has been an increasing selection of passive radar signal sources, and the emerging 5G has the characteristics of high frequency band, high bandwidth, and a large number of base stations, which make it have significant advantages in passive radar. Therefore, this paper introduces a passive radar target detection method based on 5G signals and designs a rotating target speed measurement experiment. In the experiment, this paper validated the method of de-tecting rotating targets using 5G signals and evaluated the measurement accuracy, providing a research foundation for passive radar target detection using 5G signals and detecting rotating targets such as drone rotors.

This study presents a comprehensive ecological evaluation of avian species based on 5,322 photographs obtained through camera-trap sampling. We identified 1,427 independent bird sightings, encompassing 26 families and 49 species. The study focused on temporal activity patterns, nesting behaviors, habitat preferences, and the overlap coefficient of activity patterns among 22 species of Passeriformes. Two species exhibited predominant morning activity, while five species were active in the afternoon, and 15 exhibited cathemeral activity (activity throughout the day). A cross-analysis revealed varying degrees of overlap in the activity patterns of pairs of species with similar behavioral ecology. Our findings indicate that despite exhibiting similar ecological behavior, these species display unique activity patterns, likely influenced by factors such as resource availability, competition avoidance, and thermoregulation strategies. The results highlight the richness and complexity of avian temporal niches and emphasize the need for further research into their correlation with environmental factors. This study contributes to a deeper understanding of niche separation within Passeriformes and expands our knowledge of avian behavioral ecology.

This study investigates the applications of reconfigurable intelligent surfaces (RIS) in heterogeneous downlink networks (HetNet). Due to the network densification, the small cell base station (SBS) interferes with the macrocell users (MUE). In this paper, we utilize RIS to mitigate cross-tier interference in an HetNet via directional beamforming by adjusting the phase shift of the RIS. We consider an RIS-assisted HetNet consisting of multiple SBS nodes and MUEs that utilize both direct paths and reflected paths. Therefore, the aim of this study is to maximize the sum rate of all MUEs by jointly optimizing the transmit beamforming of the macrocell base station (MBS) and the phase shift of the RIS. An efficient RIS reflecting coefficients-based optimization (RCO) is proposed based on a successive convex approximation approach. Simulation results are provided to show the effectiveness of the proposed scheme in terms of its sum rate in comparison with the scheme HetNet without RIS and the scheme HetNet with RIS but with random phase shifts.

Three-dimensional transfer functions (3D TFs) are generally assumed to fully describe the transfer behavior of optical topography measuring instruments such as coherence scanning interferometers in the spatial frequency domain. Therefore, 3D TFs are supposed to be independent of the surface under investigation resulting in a clear separation of surface properties and transfer characteristics. In this paper we show that the 3D TF of an interference microscope differs depending on whether the object is specularly reflecting or consists of point scatterers. In addition to the 3D TF of a point scatterer, we will derive an analytical expression for the 3D TF corresponding to specular surfaces and demonstrate this as being most relevant in practical applications of coherence scanning interferometry (CSI). We additionally study the effects of temporal coherence and disclose, that in conventional CSI temporal coherence effects dominate. However, narrowband light sources are advantageous if high spatial frequency components of weak phase objects are to be resolved, whereas for low-frequency phase objects of higher amplitude the temporal coherence is less affecting. Finally, we present an approach that explains the different transfer characteristics of coherence peak and phase detection in CSI signal analysis.

We propose the physical proof-of-concept of a new simple miniature pressure sensor based on the whispering gallery modes (WGMs) optically excited in a dielectric microsphere placed near a flexible reflective membrane, which acts as an ambient pressure sensing element. WGMs excitation is carried out by free-space coupling of optical radiation to a microsphere. The distinctive feature of proposed sensor design is double excitation of optical eigenmodes by forward and backward propagating radiation reflected from a membrane that causes WGMs interference in particle volume. The optical intensity of resulting resonant field established in the microsphere carries information about the exact position of the pressure-loaded reflecting membrane. The sensitivity of the proposed sensor strongly depends on the quality factor of the excited resonant mode, as well as geometrical and mechanical parameters of the flexible membrane. We propose to register not the displacement of the position of the WGM resonance, but the change in its amplitude under the influence of the change in the distance between the sphere and the mirror under the influence of pressure. Important advantages of the proposed sensor are miniature design (linear sensor dimensions depends only on the membrane diameter) and the absence of a mechanical contact of pressure-sensitive element with WGM resonator.

Plant pathogenic fungi are the largest group of disease-causing agents on crop plants and represent a persistent and significant threat to agriculture worldwide. Conventional approaches based on the use of pesticides raise social concern for the impact on the environment and human health and alternative control methods are urgently needed. The rapid improvement and extensive implementation of RNAi technology for various model and non-model organisms has provided the initial framework to adapt this post-transcriptional gene silencing technology for the management of fungal pathogens. In this review, we describe exogenous RNAi involved in plant pathogenic fungi and discuss small RNA production, formulation, and RNAi delivery methods. We explore some challenges with possible solutions. Furthermore, exogenous RNAi holds great potential for RNAi-mediated plant pathogenic fungal disease control.

In the context of the French-German space lidar mission MERLIN dedicated to the determination of the atmospheric methane content, an end-to-end mission simulator is being developed. In order to check whether the instrument design meets the performance requirements, simulations have to count all the sources of noise on the measurements like the optical energy variability induced by speckle. Speckle is due to interference as the lidar beam are quasi monochromatic. Speckle contribution to the error budget has to be estimated but also simulated. In this paper, the speckle theory is revisited and applied to MERLIN double pulsed IPDA lidar and also to the DLR demonstrator CHARM-F. Results show: on the signal path, speckle noise depends mainly on the size of the illuminating area on ground; on the solar flux, speckle is fully negligible both because the pixel size and the optical filter spectral width; on energy monitoring path a decorrelation mechanism is needed to reduce speckle noise on averaged data. Speckle noises for MERLIN and CHARM-F can be simulated by Gaussian noises with only one random draw by shot separately for energy monitoring and signal paths.

Although there is a several array of diagnostic and therapeutic choices for pancreatic cancer in recent years, a crucial medical approach for the refractory disease is still needed. Oligonucleotide therapeutics, such as those based on antisense RNAs, RNA interference, aptamers and decoys, are promising agents against pancreatic cancer because they identify a specific nucleotide sequence or protein and interfere with gene expression as molecular-targeted agents. Within just the past quarter-century, the diversity and feasibility of these drugs as diagnostic or therapeutic tools have dramatically increased. Actually, there have been several clinical and preclinical studies of oligonucleotides for patients with pancreatic cancer so far. To support the discovery of effective diagnostic or therapeutic options by using oligonucleotide-based strategies in the absence of satisfactory therapies for long-term survival and the rising trend of diseases, we summarize the current clinical trials of oligonucleotide therapeutics for pancreatic cancer patients with underlying preclinical or scientific data and focus on the possibility of oligonucleotides to target pancreatic cancer in clinical implications.

Slow growth of calcite in confinement is abundant in Nature and man made materials. There is ample evidence that such confined growth may create forces that fracture solids. The thermodynamic limits are well known but since confined crystal growth is transport limited and difficult to control in experiment we have almost no information on the mechanisms or limits of these processes. We present a novel approach to in situ study of confined crystal growth using microfluidics for accurate control of the saturation state of the fluid and interferometric measurement of the topography of the growing confined crystal surface. We observe and explain the diffusion limited confined growth structures observed and can measure the crystal "floating" on a fluid film of 10-40~nm thickness due to the disjoining pressure. We find that there are two end member behaviours: smooth or intermittent growth in the contact region, the latter being faster than the former.

Large Language Models (LLMs) have proved significant proficiency when comes to code generation especially in Structured Query Language (SQL) for databases and recent successful Text-to-SQL method involves fine-tuning pre-trained LLMs for SQL generation tasks. Transforming natural language text into SQL queries, has been attempted to solve with various learning techniques including Few-shot learning[1], fine tuning. In this paper we propose Supervised fine-tuning (SFT) as a better alternative for learning technique for text-to-SQL generation task using Code-Llama that pushes state of art accuracy on spider test suite to 89.6% on dev set which represent first instance of surpassing the earlier best-in-class with 5.5% higher score and 86.8% of exact match accuracy on dev set. Furthermore, we demonstrate that properly prompted LLM along with SFT provides far fewer hallucinations and much more robust LLM that can be used as a general tool for any text-to-SQL generation use case.

Recent advancements in semiconductor photonic and electronic devices such as Uni-travelling-carrier photodiodes (UTC-PDs), have played a significant role in the development of Terahertz communication technology. However, a major challenge that still persists is the limited output power from a single UTC-PD required for practical transmission distances. To enhance the output power, we propose and demonstrate a novel Terahertz wave power combining technique using a photomixer device that comprises of two arrayed UTC-PDs monolithically integrated with a microstrip line-based 2 × 1 Wilkinson power combiner and a rectangular patch antenna on a Silicon Carbide (SiC) substrate at 300 GHz. At photocurrents of 8 mA and 10 mA from the two UTC-PDs, the device experimentally exhibited a 7.3 dB increase in power relative to the power obtained with 8 mA UTC-PD and a 4.4 dB increase in power relative to the power obtained with 10 mA UTC-PD, when both UTC-PDs were irradiated. This implies that power can be enhanced by a factor of N2 if the photocurrent is multiplied by a factor of N. This technology presents a holistic and cutting edge platform for further power multiplication by further increasing the number of arrayed UTC-PDs.

The term deviation frequency denotes the boundary between the variable part of the amplitude and phase scintillation spectrum and the part of uninformative noises. We suggested the concept of the “characteristic deviation frequency” during the observation period which is defined as the most probable value of the deviation frequency under current local conditions. This work is a case study of the characteristic deviation frequency (fd) registered for GPS, GLONASS, Galileo and SBAS signals under quiet and weakly disturbed geomagnetic conditions in April 2021 at the mid-latitude GNSS station. Our results demonstrated that the fd value for all signal components of GPS, GLONASS and GALILEO signals varied within 15-22 Hz. The characteristic deviation frequency was 20 Hz for the mentioned GNSS signals. In difference, the deviation frequency was limited within 13-20 Hz for SBAS with the lower characteristic deviation frequency at 18 Hz. We assume that the concept of the characteristic deviation frequency can be used to determine the optimal sampling rate of the GNSS carrier phase data for the ionospheric studies. The characteristic deviation frequency can also characterize the state of the regular trans-ionospheric radio channel.

Based on Venturini method, it is in favor of the modulation technique for controlling the matrix converter due to only use of the comparison between the duty cycles in time domain and the triangular carrier wave for generating the gating signals and the achievable voltage ratio between fundamental output magnitude and fundamental input magnitude to 0.866. However, even with simple modulation method and achieving maximum fundamental output magnitude, the possible input voltage unbalance conditions accordingly influence on the output performances (more reduction and distortion). Thus, a modified Venturini modulation method is presented in this paper, in order to solve the problems of unbalanced input voltage conditions on the matrix converter performances. The proposed strategy is to satisfy the desirable feature of the duty cycle modulating waves, as generated in the event of normal situation. Up to this approach, it can support either single-phase condition or two-phase condition. Performance of the proposed control strategy was verified by the simulated implementation in the MATLAB/Simulink software with showing good steady-state and dynamic operations.

Global navigation satellite system (GNSS) array antenna receivers are widely used to suppress wideband interference in navigation countermeasures. However, existing array antenna receivers all adopt digital array structure and digital beamforming technique, and it has limited analog-front-end (AFE) dynamic range. In strong interference scenarios, AFE saturation will occur, which limits the maximum interference suppression ability of the array receiver. Aiming at this issue, this paper proposes a robust wideband interference suppression method for GNSS array antenna receivers based on hybrid beamforming technique. Firstly, a novel fully connected hybrid array receiver structure is proposed; Secondly, the corresponding hybrid beamforming method is proposed at the same time, and it realizes the complete elimination of strong interference by jointly suppression in analog domain and digital domain. After mathematical simulation experiments, it is verified that compared to the digital beamforming-based anti-jamming technique, the proposed method can effectively suppress strong wideband interference, and the maximum interference suppression ability is improved by 36 dB.

Sugarcane mosaic virus (SCMV) is a (genus, Potyvirus; family, Potyviridae) is a widespread, deleterious, and the most damaging pathogen of sugarcane ((Saccharum officinarum L. and Saccharum spp.) which causes a substantial barrier to producing high sugarcane earning. Sugarcane mosaic disease (SCMD) is caused by, single or compound infection of SCMV, disseminated by the several aphid vectors in a non-persistent manner. SCMV has flexuous filamentous particle of 700-750 nm long, which encapsidated a positive-sense, single-stranded RNA molecule of 9575 nucleotides. RNA interference (RNAi)-mediated antiviral innate immunity is an evolutionary conserved, key biological process in eukaryotes and has evolved as an antiviral defence system to interfere with viral genomes for controlling infections in plants. The current study aims to analyze sugarcane (Saccharum officinarum L. and Saccharum spp.) locus-derived microRNAs (sof-miRNAs/ssp-miRNAs) with predicted potential for targeting the SCMV +ssRNA-encoded mRNAs, using a predictive approach that involves five algorithms. The ultimate goal of this research is to mobilize the in silico- predicted endogenous sof-miRNAs/ssp-miRNAs to experimentally trigger the catalytic RNAi pathway and generate sugarcane cultivars to evaluate the potential antiviral resistance surveillance ability and capacity for SCMV. Experimentally validated mature sugarcane (S. officinarum, 2n = 8X = 80) and (S. spp., 2n = 100-120) sof-miRNA/ssp-miRNA sequences (n = 28) were downloaded from the miRBase database and aligned with the SCMV genome (KY548506). Among the 28 targeting mature locus-derived sof-miRNAs/ssp-miRNAs evaluated, one sugarcane miRNA homolog, sof-miR159c, was identified to have a predicted miRNA binding site, at nucleotide position 3847 of the SCMV genome targeting CI ORF. To verify the accuracy of the target prediction accuracy and to determine, whether the sugarcane sof-miRNA/ssp-miRNA could bind the predicted SCMV mRNA target(s), we constructed an integrated Circos plot. A genome-wide in-silico-predicted miRNA-mediated target gene regulatory network has implicated to validate interactions necessary to warrant in vivo analysis. The current work provides valuable computational evidence for the generation of SCMV-resistant sugarcane cultivars.

Mycoviruses are widespread across the major taxa of fungi including yeasts. Mycoviruses in the yeast fungus Geotrichum candidum are not well studied with three G. candidum-associated viral species characterized to date, all of which belong to the Totiviridae genus Totivirus. In this study, we report the molecular characteristics of another two totiviruses co-infecting isolate Gc6 of G. candidum. The two totiviruses were tentatively named Geotrichum candidum totivirus 2 (GcTV2-Gc6) and GcTV4-Gc6). Both viruses have typical genome organization of totiviruses comprising two ORFs encoding capsid protein (CP) and RNA-dependent RNA polymerase (RdRp) at the N and C termini, respectively. Genomes of GcTV2-Gc6 and GcTV4-Gc6 are 4592 and 4530 bp long, respectively. Both viruses contain the -1 frameshifting elements and their proteins could be expressed as a single fusion protein. GcTV2-Gc6 is closely related to a totivirus isolated from the same host whereas GcTV4-Gc6 is related to insect-associated totiviruses. The phylogenetic analysis indicated that GcTV2-Gc6 and GcTV4-Gc6 belong to two different sister clades, I-A and I-B, respectively. It is interesting that all viruses identified from G. candidum belong to the genus Totivirus. It is possible that the RNA interference (RNAi) mechanism is not active to suppress totivirus accumulation in isolate Gc6.

Medium-deep Borehole Heat exchanger (MBHE) has been widely paid attention for building heating. In order to avoid the thermal interference of adjacent MBHEs, temperature distribution characteristics of medium-deep rock-soil was investigated in this work. The evolution on maximum rock-soil thermal affected radius (MTAR) in full life cycle was further analyzed. The results showed that the rock-soil thermal affected area (RTAA) continuously expands in both radial and vertical directions during a heating season. Several factors of thermal extraction load, fluid velocity, geothermal gradient and pipe length have main impacts on the RTAA in vertical direction. Rock-soil thermal conductivity affects the RTAA in both radial and vertical directions. In non-heating season, temperature difference of rock-soil in radial direction dominates the temperature recovery in the first year while geothermal flow nearly dominates temperature recovery after 20 years. The thermal affected radius (TAR) in the 30 th year could be determined as an effective MTAR in full life cycle. In order to decrease installed distance of adjacent MBHEs, a new well layout form by deflecting borehole was proposed. The recommended deflection angle is equal to or larger than 4 times TAR angle. This work provides scientific reference for promoting application of multiple MBHE arrays.

The calibration of length is related to the national economy and people's livelihood. The traditional calibration of length value was sent the uncalibrated instrument to the laboratory and the intrinsic error of the tested instrument was obtained in the laboratory. However, calibrating the instrument in the site produced additional error. In this article, a remote calibration method of length measurement based on optical fiber information transmission following with a proof of principle system was studied to reduce the additional error. The 1.0-level displacement guide rail was calibrated according to this method. The results showed that the relative error of guide rail displacement under different lengths was within 0.6%. The expanded uncertainties of 10 μm, 100 μm and 1 mm were analyzed respectively. The calibration results meet the actual standards of the guide rail. This optical method is a good candidate for remote calibration.

High-performance electromagnetic interference (EMI) shielding materials with ultralow density and environment friendly properties are greatly demanded to address electromagnetic radiation pollution. Herein, carbon nanotubes/polylactic acid (CNTs/PLA) materials with different CNTs contents, which exhibit characteristics of light weight, environmental protection and good chemical stability, are fabricated by 3D printing technology, where CNTs are evenly distributed and bind well with PLA. The performances of 3D printed CNTs/PLA composite are improved compared to pure 3D printed PLA composite, which include mechanical properties, conductive behaviors and electromagnetic interference (EMI) shielding. The EMI shielding effectiveness (SE) of CNTs/PLA composite could be improved when the contents of CNTs increase. When it reaches 15 wt%, the EMI SE of 3D printed CNTs/PLA composite could get up to 47.1 dB, which shields 99.998% of electromagnetic energy. Meanwhile, the EMI shielding mechanism of 3D printed CNTs/PLA composite is mainly of absorption loss, and it generally accounted for more than 80% of the total shielding loss. These excellent comprehensive performances endow the 3D printed CNTs/PLA composite with great potential for use in industrial and aerospace area.

Sugarcane mosaic virus (SCMV) is a deleterious pathogen which causes widespread Sugarcane mosaic disease (SCMD) and is classified in the genus Potyvirus (Potyviridae), disseminated by the aphid vector. RNA interference (RNAi)-mediated antiviral innate immunity is a key biological process and antiviral defence system to interfere with viral genomes for controlling plant pathogens. The current study aims to analyze sugarcane (Saccharum officinarum L. and Saccharum spp.) locus-derived microRNAs (sof-miRNAs/ssp-miRNAs) with predicted potential for targeting the SCMV +ssRNA-encoded mRNAs, using ‘five algorithms’ approach. The ultimate goal in this research is to mobilize the in silico endogenous predicted sof-miRNAs/ssp-miRNAs to trigger RNAi catalytic pathway experimentally and generate sugarcane cultivars for evaluating potential antiviral resistance monitoring capability and capacity for SCMV. Experimentally validated mature sugarcane (S. officinarum, 2n = 8X = 80) and (S. spp., 2n = 100-120) sof-miRNAs/ssp-miRNAs (n = 28) were acquired for alignment with the SCMV genome. Of the 28 targeting mature locus-derived sof-miRNAs/ssp-miRNAs investigated, one sugarcane miRNA homolog, sof-miR159c, was concluded to localize potential binding site at genomic nucleotide site 3847 targeting CI ORF of SCMV. In order to validate target prediction accuracy, whether the sugarcane sof-miRNA/ssp-miRNA might bind predicted SCMV mRNA target(s), we created an integrated Circos plot. Genome-wide in-silico-predicted miRNA-mediated target gene regulatory network validated interactions that warrant in vivo analysis. The current work provides valuable evidence and biological material for generating SCMV-resistant sugarcane varieties.

Olfaction plays a crucial role in many important behavioral activities of insects, such as finding sexual partners, locating hosts, and selecting oviposition sites. Ionotropic receptors (IRs) play a central role in detecting chemosensory information from the environment and guiding insect behaviors and are potential target genes for pest control. Empoasca onukii Matsuda is a major pest of the tea plant Camellia sinensis (L.) O. Ktze, and seriously influences tea yields and quality. In this study, the ionotropic receptor gene EnouIR25a in E. onukii was cloned, and the expression pattern of EnouIR25a was detected in various tissues. Behavioral responses of E. onukii to volatile compounds emitted by tea plants were determined by the olfactometer bioassay and field trials. To further explore the function of EnouIR25a in olfactory recognition of compounds, RNAi (RNA interference) of EnouIR25a was carried out by ingestion of in vitro synthesized dsRNAs. The CDS length of EnouIR25a was 1266 bp and it encoded a 48.87 kD protein. EnouIR25a was enriched in the antennae of E. onukii. E. onukii were more significantly attracted by 1-phenylethanol at concentration of 100 µl/ml. Feeding with dsRNA-IR25a significantly downregulated the expression level of EonuIR25a, after 3 h of treatment, which disturbed the behavioral responses of E. onukii to 1-phenylethanol at concentration of 100µl/ml. The response rate of E. onukii to 1-phenylethanol was significantly decreased after dsRNA-IR25a treatment for 12 h. In summary, the ionotropic receptor gene EnouIR25a was highly expressed in the antennae of E. onukii and was involved in olfactory recognition of the tea plant volatile 1-phenylethanol. The present study may help us to use the ionotropic receptor gene as a target for the behavioral manipulation of E. onukii in the future.

EMG analysis is becoming increasingly important in many research and clinical applications, including muscle fatigue detection, control of robotic mechanisms and prostheses, clinical diagnosis of neuromuscular diseases and quantification of force. However, electromyographic signals can be contaminated by various types of noise, interference and artifacts, which can lead to misinterpretation of the data acquired using this method. Even assuming best practices, the collected signal may still be altered by such contaminants. The aim of this paper is to review methods employed to reduce contamination of single channel EMG signals. This review is limited to methods performed directly on the measured EMG signal and those that allow total reconstruction of the EMG signal. Subtraction methods used in the time domain, denoising methods performed after signal decomposition and hybrid methods are assessed. It is defended that individual methods may be more or less suitable for a particular application depending on contaminant(s) present in the signal and on the specific requirements of the application.

Josephson junctions (JJs) in the presence of a magnetic field exhibit qualitatively different interference patterns depending on the spatial distribution of the supercurrent through the junction. In JJs based on two-dimensional topological insulators (2DTIs), the electrons/holes forming a Cooper pair (CP) can either propagate along the same edge or be split into the two edges. The former leads to a SQUID-like interference pattern, with the superconducting flux quantum $\phi_0$ (where $\phi_0=h/2e$) as a fundamental period. If CPs’ splitting is additionally included, the resultant periodicity doubles. Since the edge states are typically considered as being strongly localized, the critical current does not decay as a function of the magnetic field. Here we go beyond this approach and inspect a topological JJ in the tunneling regime featuring extended edge states. We consider the possibility that the two electrons of a CP propagate and explore the junction independently over length scales comparable with the superconducting coherence length. As a consequence of the spatial extension, we obtain a decaying pattern with different possible periods. In particular, we show that, if crossed Andreev reflections (CARs) are dominant and the edge states overlap, the resulting interference pattern features oscillations whose periodicity approaches $2\phi_0$.

Pratylenchus loosi is an important root-lesion nematode that cause damage to tea plantations in Iran and all over the world. The present study reports on the characterization and evolution of three ß-1,4-engoglucanase genes Pl-eng-2, Pl-eng-3 and Pl-eng-4. The gene structure of Pl-eng-2 was fully determined with the predicted signal peptide and devoid of the linker domain and carbohydrate-binding domain, while Pl-eng-3 and Pl-eng-4 were only partially sequenced. The transcription of Pl-eng-2 was localized in the secretory esophageal glands of all life stages, but it was upregulated in male and females stages. Exon/intron structures of Pl-eng-2, Pl-eng-3 and Pl-eng-4 confirmed that they resulted from gene duplication followed by sequence and gene structure diversification with loss of linker domain and carbohydrate-binding domain during evolution. Phylogenetic analysis further confirmed that nematode endoglucanases resulted from horizontal gene transfer of a bacterial gene as Pl-eng-3 showed sister relationships with CelB cellulase of Bacillus subtilis. Silencing Pl-eng-2 by in vitro RNA interference, produced a 60% decrease of the transcript level. The reproductive ability of silenced P. loosi showed a 35% reduction of eggs and larval stages compared to untreated nematodes suggesting that this gene is involved in the early steps of invasion.

In this paper, we first investigate secure communications for a two-hop interference relay system in the wireless Internet of Things (IoT), where K source-destination pairs communicate in the presence of an eavesdropper. Explicitly, we jointly design source, relay and destination matrices upon minimizing total mean-squared error (MSE) of all legitimate destinations while keeping the MSE at eavesdropper above a certain threshold. We illuminate that the joint design of the source, relay and destination matrices subject to both secrecy and transmit power constraints. More specifically, we proposed an efficient iterative distributed algorithm to simplify the process of the joint design for optimal source, relay and destination matrices. Furthermore, the convergence of the iterative distributed algorithm is described. Additionally, the performances of the proposed algorithm, including both its secrecy rate and MSE, are characterized with the aid of simulation results. We demonstrate that our proposed algorithm outperforms the traditional approach. As a benefit, secure communications can be guaranteed by using the proposed algorithm for the multiple input multiple output (MIMO) interference relay IoT network in the presence of an eavesdropper.

In modern Commercial Off-The-Shelf (COTS) multicore systems, cores can produce several simultaneous memory requests. The processing of such requests over the memory controller negatively impacts the interference delay triggered by running parallel tasks on the platform. In this paper, we propose a software-based testing approach for analyzing memory interference delay, when cores are exposed to extensive read/write requests that access in parallel their Cache Coherent Interconnect. The hardware targeted in this work is the well-known LayerScape QorIQ LS2085A, which can be approached as a potential successor to the Freescale QorIQ P4080. The test analysis was conducted based on a bare-metal operating system that we developed to guarantee a deterministic execution environment at all time points. Our testing was accomplished using a set of carefully designed synthetic benchmarks as well as TACLeBench benchmarks.

This paper presents the design and measurement of an IEEE 802.11ad standard compatible RF transceiver for 60GHz wireless communication system. In addition to the traditional half-duplex (HD) mode, this work supports full-duplex (FD) operation to deliver better channel utilization and faster response time for the system. The isolation between the transmitter and receiver from the architecture design to system integration for FD operation has been fully considered. A digital self-interference cancellation (DSIC) is implemented in MATLAB to verify the FD performance. The super-heterodyne architecture with an intermediate frequency (IF) of 12GHz is designed to suppress the image frequencies without using extra filters. A flexible phase-locked loop (PLL) synthesizer provides a local oscillator (LO) frequency with a 2kHz resolution. Other than the time division duplex (TDD) mode used in the conventional 60GHz system, a wide bandwidth baseband digital variable gain amplifier (DVGA) with a 3dB bandwidth of more than 4GHz also supports frequency division duplex (FDD) operation. The transceiver chip is fabricated in Tower Jazz 0.18µm SiGe BiCMOS process. With on-board antenna, the transceiver covers all four channels in 802.11ad standard with MCS-12 (7.04Gbps under 1.76GSym/s and 16-QAM) under 1.5m. In the proposed system design, the RF frontend based self-interference (SI) suppression from the local transmitter to receiver LNA is around 54dB. To achieve a practical FD application, the SI is further suppressed with the help of digital SI compensation. The measured power consumption for transmitter and receiver configuration is 194mW and 231mW, respectively, in HD mode and 398mW for FDD or FD operation mode.

Nanocrystalline silicon oxide (nc-SiOx:H) is a multipurpose material with its varied application in solar cells as a transparent front contact, intermediate reflector, back reflector layer and even tunnel layer for passivating contacts, owing to easy tailoring of its optical properties. In this work, we systematically investigate the influence of gas mixture (SiH4, CO2, PH3 and H2), RF power and process pressure on the optical, structural and passivation properties of thin n-type nc-SiOx:H films prepared in an industrial, high throughput, plasma-enhanced chemical vapor deposition (PECVD) reactor. We provide a detailed description of the n-type nc-SiOx:H material develop-ment using various characterization techniques (SEM, EDX, Raman spectroscopy, and spectro-scopic ellipsometry) with a focus on the relationship between the material properties and the pas-sivation they provide to n-type c-Si wafers characterized by their effective carrier lifetime (τeff). Further, we also outline the parameters to be kept in mind while developing different n-type nc-SiOx:H layers for different solar cell applications. We report a tunable optical gap (1.8 – 2.3 eV) for our n-type nc-SiOx:H films as well as excellent passivation properties with τeff up to 3.5 ms (iVoc ~ 715 mV) before annealing. Oxygen content plays an important role in determining the crystallinity and hence passivation quality of the deposited nanocrystalline silicon oxide films.

It is well known that the event of hepatitis B virus reactivation can occur among patients undergoing treatment for hematological malignancies. In this paper we will present the available data regarding the risk of hepatitis B virus reactivation in this special population of immunosuppressed patients and explore the relevance of an accurate prevention and management of this condition. A computerized literature search was performed using appropriate terms arrangement, including English-written literature only or additional relevant articles. The evaluation of hepatitis B reactivation risk is a multidimensional process, which includes conducting an accurate clinical and physical history, considering the virological categories, the knowledge of the medication chosen to treat these hematological malignancies and the induced grade of immunosuppression. Adopting adequate preventive strategies and surveillance according to the current international recommendations is crucial to prevent HBVr and its dire clinical consequences (hepatitis, liver failure, interruption of lifesaving anti-neoplastic treatments). Universal HBV screening of patients scheduled to undergo treatment for hematological malignancies should be the chosen policy, and clinicians should be aware of the inherent risk of viral reactivation among the different virological categories and the classes of immunosuppressive drugs.

The technique of optical interferometry has many applications in various fields of knowledge, including astronomy, optical metrology, biology, and chemistry. The visible interference pattern of a conventional single mode fiber (SMF-28) tapered and core-offset structured in-line Mach-Zehnder interferometer has been demonstrated. The white source is generated by an LED, with a wavelength range of 400 nm to 650 nm emission. We analyze the performance of the interference emission with a waist length of 1.8 mm to 6 mm and with a waist diameter of 10 µm to 60 µm for the tapered structure. The achieved visibility is V=0.14 with an FSR of 23 nm. The core-offset structure interferometer is analyzed with different displacements in x and y axis and with a separation length L of 2.5 cm, 4.0 cm and 5.0 cm between two core joints. The output interference emission has a visibility of V= 0.3, V=0.27 and V=0.34 with an FSR of 23 nm, 17 nm and 8 nm, respectively. The sensitivity obtained from the temperature sensor is of 3.53 a.u./°C, with R2 of 0.99769. The proposed MZIs are low cost and can be used for implementation of sensing system in the field as the industry and medical applications.

Tomato (Solanum lycopersicum L.) is one of the most commercially essential vegetable crops cultivated worldwide. In addition to the nutritional value, tomato is an excellent model for studying climacteric fruits' ripening process. Despite this, the available natural pool of genes that allows expanding phenotypic diversity is limited, and the difficulties of crossing using classical selection methods when stacking traits increase proportionally with each additional feature. Genetic engineering makes it possible to introduce changes in genes of interest without changing the allelic combinations characteristic of successful commercial varieties, to enhance the expression of existing genes, to create and transfer artificial and heterologous genes, and much more. However, this requires understanding the fundamental principles of the molecular interaction. Although the leading candidate genes for these components have been identified, a complete picture of their relationship has yet to be formed. The review summarizes the latest (2017-2023) achievements related to studying the ripening processes of tomato fruits. In this work, an attempt is made to systematize the results of various research and display the interaction pattern of genes regulating the process of tomato fruit ripening.

The chitin synthase B gene is a key enzyme in the chitin synthesis of insect peritrophic matrix (PM), affecting insects' feeding behavior. The chitin synthase B gene has been cloned from the transcription library of Heortia vitessoides Moore. RT-qPCR showed that HvChsb was highly expressed in the larval stage of H. vitessoides, especially on the first day of the prepupal stage, and in the midgut of larvae and the abdomen of adults. After starvation treatment, HvChsb was found to be significantly inhibited with time. After 48 h of starvation, the feeding experiment showed that HvChsb increased with the prolongation of the refeeding time. The experimental data showed that feeding affected the expression of HvChsb. HvChsb was effectively silenced by RNA interference, so its function was lost, significantly decreasing the survival rate of H. vitessoides. The survival rate from larval-to-pupal stages was only 43.33%, accompanied by abnormal phenotypes. It can be seen that HvChsb plays a key role in the average growth and development of H. vitessoides.

In this paper, we propose a multi-zone service control scheme to maximize the performance of each service zone when a large number of cellular service zones and D2D (Device-to-Device) service zones are composed to 5G cellular network. This paper also improves performance of service zone by dividing traffic into real-time traffic and non-real-time traffic in order to minimize traffic interference. Real-time traffic and non-real-time traffic have a significant impact on communication performance. We propose a new self-detection traffic interference control technique to improve the QoS and throughput of D2D and C2D communication in a cellular network, STICS (Self-detecting Traffic Interference Control Scheme). The proposed STICS scheme distinguishes between short-term traffic congestion process and long-term traffic congestion process according to traffic characteristics to detect and control traffic. When the proposed scheme is applied to the 5G-based cellular network environment, it is expected that the traffic type will be efficiently classified by self-detecting the traffic according to the flow. Such classified traffic is less sensitive to communication between the D2D and C2D links, thereby reducing traffic overload. We evaluate the performance of the proposed scheme through simulation and show that the proposed scheme is more efficient than other comparison schemes.

A well known result for the interference of two single-mode fields is that the degree of coherence and the degree of indistinguishablity are same when we consider the detection of a single photon. In this article we present the relation between degree of coherence, path indistinguishability and the fringe visibility considering interference of multiple number of single-mode fields while being interested in the detection of a single photon only . We will also mention how Born’s rule of interference for multiple sources is reflected in these results.

Measurement-Device-Independent Quantum Key Distribution (MDI-QKD) is a two-photon protocol devised to eliminate eavesdropping attacks that interrogate or control the detector in realized quantum key distribution systems. In MDI-QKD, the measurements are carried out by an untrusted third party, and the measurement results are announced openly. Knowledge or control of the measurement results gives the third party no information about the secret key. Error-free implementation of the MDI-QKD protocol requires the crypto-communicating parties, Alice and Bob, to independently prepare and transmit single photons that are physically indistinguishable, with the possible exception of their polarization states. In this paper, we apply the formalism of quantum optics and Monte Carlo simulations to quantify the impact of small errors in wavelength, bandwidth, polarization and timing between Alice's photons and Bob's photons on the MDI-QKD quantum bit error rate (QBER). Using published single-photon source characteristics from two-photon interference experiments as a test case, our simulations predict that the finite tolerances of these sources contribute (4.04+/-20/N_sifted) to the QBER in an MDI-QKD implementation generating an N_sifted-bit sifted key.

Non-viral gene carriers because of their limited side effects, biocompatibility, simplicity and taking the advantages of electrostatic interactions have shown noticeable potential in gene delivery. The low transfection rate of non-viral vectors under physiological conditions is a significant issue. Here, the aim of this study was to investigate the efficacy of hydrophilic and hydrophobic groups on gene carriers such as two synthesized amphiphilic polymer of dextran-stearic acid-spermine (DSASP) with verified lipid and amine conjugations that associated with Fe3O4 superparamagnetic nanoparticles to promote the target delivery and decrease the transfection time using static magnetic field. Our findings illustrate that magnetic nanoparticles are spherical with positive surface charges and superparamagnetic behaviors. The DSASP–pDNA/MNPs offered a strong pDNA condensation, protection against DNase degradation, significant cell viability in HEK 293T cells and. Although conjugations of spermine play a critical role in transfection efficiency, amphiphilic polymer with more derivatives of stearic acid showed better transfection yields. Therefore, DSASP amphiphilic magnetic carriers offer new insights for gene delivery due to the amine contents and ameliorate the uptake of complexes via cell membrane based on its hydrophilic surface.

The part transportation efficiency is a main factor of aircraft sortie generation rate. Part transportation is used to transport spare part from base to carrier. Transportation strategy depends on both demand on carrier and inventory in transportation base. The transportation time and stochastic demand will induce fluctuations of cost and inventory. Thus, a Takagi-Sugeno fuzzy system of dynamic part transportation is established considering transportation time and stochastic demand. And a novel Takagi-Sugeno fuzzy robust control is designed for dynamic part transportation, which will keep transportation cost and part inventory stable. First of all, a fuzzy model with stochastic demand and transportation time is proposed. Then, a novel robust control with cross rule groups is conducted according to production and transportation strategy, which will reduce fluctuations induced by strategies switch. Moreover, robust stability is guaranteed and part can be supplied in time under a low cost. Finally, simulation illustrates usefulness and quickness of the novel Takagi-Sugeno fuzzy robust control. Besides, the proposed method will be useful in other transportation electrification systems with delay time and uncertainty.

Some of the European countries affected by COVID-19 have not learned from previous experience in China. Italy did not learn from China and Spain did neither learn from China nor from Italy. Confinement in Spain was postponed due to pressure from economic interests and traveling of infected people, especially from Madrid to the beaches in South-East Spain, was allowed. Strict confinement and border closure came late, when the curve of infected people and the death toll already had the worse trend worldwide. Tests to SARS-CoV-2 RNA detection by PCR, were first unavailable and, later, faulty and/or detecting antibodies and not the virus itself. Instead of mobilizing research laboratories for making tests, and instead of making masks and ventilators, mediatic scientists asked for money for controversial clinical trials and for obtaining a vaccine. In this scenario, common sense indicates that ad hoc measures should be taken at the end of confinement in order to minimize pain. The chain of errors should be avoided in the management of next pandemics by designing Good Practice Rules (GPRs). In addition, post-confinement measures should be implemented as soon as possible to be ready for SARS-CoV-2 return next season. In this sense a longitudinal study in the most affected cities (Madrid, Barcelona, Vitoria and Pamplona) should be performed with the primary objective of detecting carriers with no symptoms, to stratify patients according to symptoms, and to early detection of virus reappearance. Also relevant is to sequence as many viruses as possible to detect possible variants; there are still patients that are PCR positive.

The research examines the relationship between self-rated health situation and personal percep-tion of heat waves, and how social linkage of communities would be a moderator variable in residents’ perception of heat waves in Taiwan. This study uses the questionnaire conducted by Sinica “Responsive Capacity under Heat Wave: The Perspectives of the Locals”(2019), using OLS method for estimating the unknown parameters in multiple regression model. The author finds that the correlation of self-rated health situation and perception toward heat is significantly posi-tive. Also, social linkage in communities affects strongly as a moderator variable: While the sat-isfaction to with their community could reduce negative reaction to heat, contacts with neighbors could increase possibility people feel uncomfortable in high-temperature situation. This study ex-hibits the effects of social environment on community, and expects further related researches or practices to strengthen capability to resist heat wavesƒ for Taiwanese residents.

Amebiasis is a disease caused by the unicellular parasite Entamoeba histolytica. In most cases, the infection is asymptomatic but when symptomatic, the infection can cause dysentery and invasive extraintestinal complications. In the gut, E.histolytica feeds on bacteria. Increasing evidences support the role of the gut microbiota in the development of the disease. In this review we will discuss the consequences of E.histolytica infection on the gut microbiota. We will also discuss new evidences about the role of the gut microbiota in regulating the resistance of the parasite to oxi-dative stress and its virulence.

The chloroplast is important organelle responsible for photosynthesis and various bio- synthetic functions essential to green plants. However, the regulatory mechanism of chloroplast development remains elusive. Here, we characterized a mutant with defective chloroplasts in rice (Oryza sativa L.) named it albino and lethal 13(osal13), which exhibited a distinct albino phenotype in leaves, eventually leading to seedling lethality. Molecular cloning revealed that OsAL13 encodes a novel protein in rice for which no homologous gene or known conserved domain was found. Analysis of the biological characteristics showed that the RNAi2 leaf was completely albino phenotype, the root and shoot lengths were significantly inhibited compared with wild type. Transmission electron microscopy indicated that RNAi2 were defective in chloroplast development. Overexpression of OsAL13 resulted the photosynthetic rate increase, So the total grain number per panicle also would be significantly increase. Besides, OsAL13 was located in chloroplast and constitutively expressed in various tissues, including green and non-green tissues. In addition, disruption of OsAL13 resulted in altered expression of plastid-encoded polymerases and nuclear-encoded chloroplast genes, OsAL13 also affected hormone levels in plant tissues. Therefore, our results provide new insights into the role of OsAL13 in regulating chloroplast development in rice.

Aeromagnetic compensation plays a vital role in geomagnetic navigation and has received considerable attention throughout last few decades. Classical aeromagnetic compensation methods based on the Tolles–Lawson (T-L) model are mainly aimed at permanent, induced, and eddy-current magnetic interferences of aircraft platform, which ignores other stray magnetic field interference on the platform including the interferences caused by on-board electronic (OBE) systems. In order to cooperate with TL model, magnetometers are usually required to be installed on the extension rod outside the cabin, which is widely applied to geophysical magnetic survey. In order to ensure safety and reduce the cost of platform modification in geomagnetic navigation, it’s necessary to place magnetometers inside the cabin. It also further exacerbates the magnetic interferences and improves the difficulty of magnetic compensation. In this paper, a modified aeromagnetic compensation method is proposed, and the in-cabin OBE interferences are respectively modelled to be proportional to the currents and their temporal variations of different electronic devices. To ensure that modified model adapts to strong OBE interference in the cabin, a cut-off frequency determination method based on curvature calculation and a feature selection method based on correlation calculation are proposed. The cut-off frequency determination method helps to select passband filter which suitable for in-cabin OBE interference. The feature selection method can help to effectively select current and voltage inputs for modified model. In addition, principal component analysis (PCA) is adopted to reduce multicollinearity which is intensified by the extension of OBE interferences in coefficient-estimating. Experiments on public dataset are conducted to illustrate the effectiveness of the proposed method, and the best compensation result achieved 1.71nT of root mean square error (RMSE).

Electromagnetic cloaking approach is employed with the intention to curb the destructive effects of mutual interference for rectangular and circularly shaped patch antennas situated in a tight spacing (sub-wavelength separation is employed). Primarily, we show that by coating the top surface of each patch with an appropriately designed metasurface, it is possible to considerably reduce the mutual coupling between the said antennas. We also demonstrate that, despite the close proximity of these patches, the individual radiation characteristic of each antenna is reinstated to emulate their performance in an isolated scenario. Furthermore, the cloak construct is extended to a tightly spaced, interleaved linear patch antenna array configuration and it is shown that the coated metasurfaces successfully enhance the performance of each array in terms of their matching characteristics, total efficiencies as well as their far-field realized gain patterns for a broad range of beam-scan angles. The decoupling and cloaking action of these metasurfaces are demonstrated by various full-wave numerical simulation results. We believe that the simple planar structure of our metasurface design (according to simulation models) makes it feasible for fabrication and the fact that it is used to accomplish cloaking of an electrically large antenna surface, makes it unique in itself.

Apple chlorotic leaf spot virus (ACLSV) is a widespread, deleterious and the most damaging pathogen of fruit tree plants including domesticated apple (Malus domestica) ―a great threat to apple production worldwide. The positive-sense single-stranded RNA genome of ACLSV (7.5 Kbp) encodes three proteins: RNA polymerase (Rep), movement protein (MP) and coat protein (CP). RNA interference (RNAi)-mediated antiviral innate immunity is a key sequence-specific biological phenomenon in eukaryotes to control plant viruses. The aim of this study was to analyze apple (M.domestica) locus-derived microRNAs (mdm-miRNAs) with predicted potential for targeting the ACLSV +ssRNA-encoded mRNAs, using ‘four algorithms’ approach. The ultimate goal in this research is to mobilize the in silico endogenous predicted mdm-miRNAs to trigger RNAi catalytic pathway experimentally and generate apple tree varieties for evaluating potential antiviral resistance monitoring capability and capacity for ACLSV. Experimentally validated mature apple (M.domestica, 2n = 2X = 34) mdm-miRNAs (n = 322) were acquired from miRBase database and tested for alignment with the ACLSV genome. Of the 322 targeting mature locus-derived mdm-miRNAs investigated, nine apple mdm-miRNA homologs (mdm-miR395k, mdm-miR5225c and mdm-miR7121 (a, b, c, d, e, f, g, h) were predicted by all ‘four algorithms’. Only fifty eight mdm-miRNAs were predicted consensus binding sites by union of consensus between two algorithms. The miRanda, RNA22, TAPIR algorithms predicted binding of mdm-miR395k at unique nucleotide position 4691, as the most effectively interacting mdm-miRNAs in targeting the ORF1 sequence. In order to validate target prediction accuracy, whether the apple mdm-miRNAs might bind predicted ACLSV mRNA target(s), we created an integrated Circos plot. Genome-wide in-silico-predicted miRNA-mediated target gene regulatory network validated interactions that warrant in vivo analysis. The current work provides valuable evidence and biological material for generating ACLSV-resistant apple varieties.

The effect of the communication channel size on the transport and subsequent detection of chemical messengers is investigated on millimetric and micrometric channels. The transport of the information carriers, being characterized by an advective and a diffusive contribution, was simulated by varying the flow velocity and the diffusion coefficient. Then, to evaluate the information quality, the Intersymbol Interference (ISI) between two consecutive signals at a specific release delay was estimated. This allowed us to verify that operating under micrometric channel conditions has a larger flow velocity range to obtain completely separated successive signals and smaller release delays can be used between signals. The theoretical results were confirmed by developing a prototype molecular communication platform operating under microfluidic conditions, which enables communication through fluorescent nanoparticles, namely Carbon Quantum Dots (CQDs).