Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Estimating the Two Graph Dextran-Stearic Acid-Spermine Polymers Based on Iron Oxide Nanoparticles as Carrier for Gene Delivery

Version 1 : Received: 27 May 2021 / Approved: 31 May 2021 / Online: 31 May 2021 (08:25:25 CEST)

How to cite: Kazemi Ashtiyani, M.; Hajipour-Verdom, B.; Satari, M.; Abdolmaleki, P.; Hosseinkhani, S. Estimating the Two Graph Dextran-Stearic Acid-Spermine Polymers Based on Iron Oxide Nanoparticles as Carrier for Gene Delivery. Preprints 2021, 2021050709 (doi: 10.20944/preprints202105.0709.v1). Kazemi Ashtiyani, M.; Hajipour-Verdom, B.; Satari, M.; Abdolmaleki, P.; Hosseinkhani, S. Estimating the Two Graph Dextran-Stearic Acid-Spermine Polymers Based on Iron Oxide Nanoparticles as Carrier for Gene Delivery. Preprints 2021, 2021050709 (doi: 10.20944/preprints202105.0709.v1).

Abstract

Non-viral gene carriers because of their limited side effects, biocompatibility, simplicity and taking the advantages of electrostatic interactions have shown noticeable potential in gene delivery. The low transfection rate of non-viral vectors under physiological conditions is a significant issue. Here, the aim of this study was to investigate the efficacy of hydrophilic and hydrophobic groups on gene carriers such as two synthesized amphiphilic polymer of dextran-stearic acid-spermine (DSASP) with verified lipid and amine conjugations that associated with Fe3O4 superparamagnetic nanoparticles to promote the target delivery and decrease the transfection time using static magnetic field. Our findings illustrate that magnetic nanoparticles are spherical with positive surface charges and superparamagnetic behaviors. The DSASP–pDNA/MNPs offered a strong pDNA condensation, protection against DNase degradation, significant cell viability in HEK 293T cells and. Although conjugations of spermine play a critical role in transfection efficiency, amphiphilic polymer with more derivatives of stearic acid showed better transfection yields. Therefore, DSASP amphiphilic magnetic carriers offer new insights for gene delivery due to the amine contents and ameliorate the uptake of complexes via cell membrane based on its hydrophilic surface.

Subject Areas

Gene delivery; Non-viral carrier; Dextran-stearic acid-spermine; Iron oxide nanoparticles; Static magnetic field.

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