Balieiro Neto, G.; Engracia Filho, J.R.; Budino, F.E.L.; Freitas, A.W.P.; Soares, W.V.B. Effects of High-Biotin Sample Interference on Antibody Concentrations in Sandwich Immunoassays. Vaccines2023, 11, 1627.
Balieiro Neto, G.; Engracia Filho, J.R.; Budino, F.E.L.; Freitas, A.W.P.; Soares, W.V.B. Effects of High-Biotin Sample Interference on Antibody Concentrations in Sandwich Immunoassays. Vaccines 2023, 11, 1627.
Balieiro Neto, G.; Engracia Filho, J.R.; Budino, F.E.L.; Freitas, A.W.P.; Soares, W.V.B. Effects of High-Biotin Sample Interference on Antibody Concentrations in Sandwich Immunoassays. Vaccines2023, 11, 1627.
Balieiro Neto, G.; Engracia Filho, J.R.; Budino, F.E.L.; Freitas, A.W.P.; Soares, W.V.B. Effects of High-Biotin Sample Interference on Antibody Concentrations in Sandwich Immunoassays. Vaccines 2023, 11, 1627.
Abstract
The use of antibiotics grown promoter has been banned, due led problems with drug residues in animal products and increased bacterial resistance. For several reasons there is a growing interest in the scientific community in immunoglobulin Y as antibiotic alternative and their oral administration in the polyclonal antibody (pAb) format, to maintain animal health and performance, do not require IgY purification for large-scale production, resulting in protein impurities and high concentration of biotin in the samples. The signal amplification through non-covalent interaction of biotin with streptavidin has been extensionally used by many laboratories for several diagnosis diseases and scientific research in enzyme-linked immunosorbent assay (ELISA). However, the riches sources of biotin as egg yolk, leading to high biotin concentrations in samples, harming the accuracy of diagnostic and proteins concentrations tests. This study aimed to evaluate the biotin influence on measurement of Immunoglobulin Y in egg yolk freeze dried samples from immunized laying hens by immunoassays using biotin-avidin/streptavidin. The IgY concentration changed down in immunoassays using biotin-avidin/streptavidin. The detection of IgY in yolk samples by ELISA using streptavidin–biotin binding as part of the assay methodology requires some technology to neutralize high concentration of biotin on sample or more steps beyond delipidation to isolate the target protein. Otherwise, an ELISA without the use of streptavidin-biotin binding would be more advisable to avoid biotin and targets protein relationship and prevent biotin interference on results.
Medicine and Pharmacology, Dietetics and Nutrition
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