Chen, Q.; Sun, M.; Wang, H.; Liang, X.; Yin, M.; Lin, T. Characterization of Chitin Synthase B Gene (HvChsb) and the Effects on Feeding Behavior in Heortia vitessoides Moore. Insects2023, 14, 608.
Chen, Q.; Sun, M.; Wang, H.; Liang, X.; Yin, M.; Lin, T. Characterization of Chitin Synthase B Gene (HvChsb) and the Effects on Feeding Behavior in Heortia vitessoides Moore. Insects 2023, 14, 608.
Chen, Q.; Sun, M.; Wang, H.; Liang, X.; Yin, M.; Lin, T. Characterization of Chitin Synthase B Gene (HvChsb) and the Effects on Feeding Behavior in Heortia vitessoides Moore. Insects2023, 14, 608.
Chen, Q.; Sun, M.; Wang, H.; Liang, X.; Yin, M.; Lin, T. Characterization of Chitin Synthase B Gene (HvChsb) and the Effects on Feeding Behavior in Heortia vitessoides Moore. Insects 2023, 14, 608.
Abstract
The chitin synthase B gene is a key enzyme in the chitin synthesis of insect peritrophic matrix (PM), affecting insects' feeding behavior. The chitin synthase B gene has been cloned from the transcription library of Heortia vitessoides Moore. RT-qPCR showed that HvChsb was highly expressed in the larval stage of H. vitessoides, especially on the first day of the prepupal stage, and in the midgut of larvae and the abdomen of adults. After starvation treatment, HvChsb was found to be significantly inhibited with time. After 48 h of starvation, the feeding experiment showed that HvChsb increased with the prolongation of the refeeding time. The experimental data showed that feeding affected the expression of HvChsb. HvChsb was effectively silenced by RNA interference, so its function was lost, significantly decreasing the survival rate of H. vitessoides. The survival rate from larval-to-pupal stages was only 43.33%, accompanied by abnormal phenotypes. It can be seen that HvChsb plays a key role in the average growth and development of H. vitessoides.
Biology and Life Sciences, Biochemistry and Molecular Biology
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