Search | Preprints.org

Preprint ARTICLE | doi:10.20944/preprints202405.1896.v1

The Effect of Silymarin on Ischemia-Reperfusion Injury in Skeletal Muscles of Rats

Abdullah Özer, Başak Koçak, Mustafa Arslan, Barış Mardin, Ayşegül Küçük, Şaban Cem Sezen, Mustafa Hakan Zor, Mustafa Kavutçu

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: silymarin; malondialdehyde; catalase; ischemia-reperfusion

Online: 29 May 2024 (07:22:24 CEST)

Show abstract| Download PDF| Share

Background and Aim: It is generally accepted that oxidative stress mediators play an important role in ischemia-reperfusion injury(I/R), although the exact mechanism is not fully elucidated. While the impact of silymarin on I/R injury in several tissues, including the myocardial, cerebral, gastric and liver tissue, has been examined, the present state of research on this topic remains insufficient to provide a widely accepted understanding. For this purpose, our objective was to examine the effect of silymarin on muscle tissue in rats subjected to lower extremity I/R injury. Method: After ethics committee approval, 18 male Wistar albino rats weighing between 225-275 g were used. The rats were divided into three groups containing six mice each, Control,Ischemia-Reperfusion and Silymarin-Ischemia/Reperfusion groups. Silymarin was administered intraperitoneally 30 minutes before the procedure.( 100 mg/kg-1 ) In the I/R groups, an atraumatic microvascular clamp was placed in the infrarenal abdominal aorta. After 120 minutes, the clamp was removed and reperfusion was achieved for 120 minutes. In muscle tissue samples taken at the end of the reperfusion period; Malondialdehyde (MDA) levels, catalase (CAT) enzyme activity levels and histopathological parameters were compared. Results: In the histopathological examination, no degeneration was observed in the muscle fibers in the control group, while findings of striated muscle damage such as muscle atrophy hypertrophy, muscle degeneration-congestion, internal of the muscle nucleus-oval-central nucleus, fragmentation-hyalinization and leukocyte cell infiltration were observed in the I/R group. In the S-I/R group, muscle atrophy hypertrophy, internal of the muscle nucleus-oval-central nucleus, fragmentation-hyalinization and leukocyte cell infiltration were observed to improve these damaged areas compared to the I/R group. MDA levels in the I/R group were found to be significantly higher than in the control and S-I/R groups. CAT enzyme activity was found to be significantly higher in the I/R group than in the control group. Conclusion:Our study revealed that the administration of silymarin at a dosage of 100 mg/kg-1 by intraperitoneal injection 30 minutes before ischemia in rats effectively decreased lipid peroxidation and oxidative stress. Additionally, it successfully reduced the injury caused by ischemia/reperfusion in muscle histology.

Preprint ARTICLE | doi:10.20944/preprints202305.0386.v1

The Power of Nutraceuticals Fighting Liver Ischemia–Reperfusion Injury Through Apoptosis Reduction

Carlos Andrés Pantanali, Vinicius Rocha-Santos, Márcia Saldanha Kubrusly, Inar Alves Castro, Luiz Augusto Carneiro-D’Albuquerque, Flávio Henrique Galvão

Subject: Medicine And Pharmacology, Transplantation Keywords: nutraceuticals; liver; ischemia-reperfusion injury; apoptosis

Online: 6 May 2023 (05:46:56 CEST)

Show abstract| Download PDF| Share

Working Paper REVIEW

Therapeutic Effect of Mesenchymal Stem Cell on Organ Ischemia-Reperfusion Injury

Joan Oliva

Subject: Medicine And Pharmacology, Transplantation Keywords: ischemia-reperfusion injury; mesenchymal stem cells; treatment

Online: 11 June 2019 (04:28:04 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202403.1456.v1

The Effect of “Proanthocyanidin” on Ischemia-Reperfusion Injury in Skeletal Muscles of Rats

Abdullah Özer, Başak Koçak, Şaban Cem Sezen, Mustafa Arslan, Mustafa Kavutçu

Subject: Medicine And Pharmacology, Surgery Keywords: Ischemia reperfusion; proanthocyanidin; lower extremity; rat; TBARS

Online: 25 March 2024 (08:53:48 CET)

Show abstract| Download PDF| Share

BACKGROUND: Lower limb skeletal muscle ischemia-reperfusion (IR) injury associated with increased morbidity and mortality is common in several clinical situations such as aortic aneurysms repairment, peripheral arterial surgery, vascular injury repairment, and shock. Although it is generally accepted that oxidative stress mediators have a significant role in IR injury, its precise mechanism is still unknown. Anecdotally, it is sustained not only by structural and functional changes in the organ it affects but also by damage to distant organs. The purpose of this report is to illustrate the effect of proanthocyanidin on IR injury. METHODS: In our study, 18 male Wistar albino rats were used. The subjects were divided into three groups of six each. (Group Control-C, Group Ischemia-Reperfusion -IR, Group Ischemia-Reperfusion and Proanthocyanidin-IR-PRO). Intraperitoneal proanthocyanidin was given to the IR and proanthocyanidin groups 30 minutes before laparotomy and 1-hour ischemia led to these two groups. After one hour, reperfusion started. Muscle atrophy-hypertrophy, muscle degeneration-congestion, fragmentation-hyalinization, muscle oval-central nucleus ratio, leukocyte cell infiltration, catalase enzyme activity, and TBARS were all examined in lower-limb muscle samples after one hour of reperfusion. RESULTS: When skeletal muscle samples were evaluated histopathologically, it was discovered that muscle atrophy-hypertrophy, muscle degeneration-congestion, fragmentation-hyalinization, and leukocyte cell infiltration with oval-central nucleus standardization were significantly higher in the IR group than group control and group IR-proanthocyanidin. Besides, oval-central nucleus standardization was significantly higher in the IR and proanthocyanidin group than in the control group. TBARS levels were significantly higher in the IR group than in the control and IR-proanthocyanidin groups while, catalase enzyme activity was found to be significantly lower in the IR group than in the control and IR and proanthocyanidin groups. CONCLUSION: As a consequence of our research, we discovered that proanthocyanidins administered before IR have a protective impact on skeletal muscle in rats. Further research in this area is required.

Preprint ARTICLE | doi:10.20944/preprints202012.0082.v1

Cytochrome P450 CYP2E1 Suppression Ameliorates Cerebral Ischemia Reperfusion Injury

Jin Yu, Hong Zhu, Mark Kindy, Saeid Taheri

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Oxidative stress; CYP2E1; Ischemia/reperfusion; Inflammation; Blood-brain barrier

Online: 3 December 2020 (11:05:57 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202304.1055.v1

A Comprehensive Overview of Innovative Approaches to Prevent Kidney Ischemia-Reperfusion Injury: Bibliometric and In Silico Analyses

Myltykbay S. Rysmakhanov, Afshin Zare, Aibolat S. Smagulov, Nurgul A. Abenova, Nadiar M. Mussin, Yerlan B. Sultangereyev, Bazylbek S. Zhakiyev, Gani K. Kuttymuratov, Mehmet Haberal, Nazanin Jafari, Hanieh Baneshi, Shabnam Bakhshalizadeh, Mahdi Mahdipour, Farhad Rahmanifar, Amin Tamadon

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: Ischemia/reperfusion injury; Molecular docking; Bibliometric analysis; Kidney reperfusion; Kidney transplantation; Renal resection

Online: 27 April 2023 (07:25:20 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Ischemia-reperfusion injury (IRI) is a complex pathophysiological process that can lead to severe and irreversible consequences, including the complete loss of renal function. While standardized strategies for preventing IRI during "back-table" and cold preservation are available, various intraoperative prevention methods have been proposed to mitigate the harmful effects of warm ischemia and reperfusion of the kidney. These methods include pharmacological agents, mechanical interventions, and surgical techniques such as renal capsulotomy, ischemic preconditioning, venous blood reperfusion, renal perfusion, and the use of tissue engineering techniques like mesenchymal stromal/stem cells and/or decellularization. In this comprehensive analysis, we provide a detailed overview of pharmacological agents and intraoperative methods and approaches for the prevention and treatment of renal IRI. We begin by discussing the mechanisms of IRI and the challenges of preventing it during surgery. Then using a bibliometric analysis and molecular docking of brand-new legends we showed eplerenone have the most binding affinity to Aldehyde dehydrogenase (AD), Estrogen receptor (ER), Klotho protein, Mineralocorticoid receptor (MR), and Toll-like receptor 4 (TLR4) for preventing IRI comparing with other available therapeutics, Benzodioxole, Hydrocortisone, Indoles, Nicotinamide adenine dinucleotide, and Niacinamide. Next, we provide a comprehensive analysis of the potential benefits and limitations of various prevention strategies, including an in-depth discussion of the mechanisms of action of pharmacological agents, mechanical interventions, and surgical techniques. Furthermore, we introduce a novel scoring system for the prevention methods of kidney IRI, which takes into account the effectiveness, feasibility, and safety of each method. We then analyze the current state of clinical research in this area and discuss the implications of these methods for future clinical practice. Overall, this article provides a valuable resource for clinicians and researchers seeking to prevent and treat renal IRI in surgical settings, and introduces a new scoring system that can be used to guide the selection of the most effective and safe prevention methods.

Preprint ARTICLE | doi:10.20944/preprints202312.1752.v1

Camphene as a Protective Agent in Myocardial Ischemia/ Reperfusion Injury

Rodopi Stamatiou, Maria Anagnostopoulou, Chrysa Rapti, Antigone Lazou

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: camphene; ischemia/reperfusion injury; oxidative stress; antioxidant activity; redox homeostasis

Online: 22 December 2023 (15:07:08 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202208.0158.v1

Regulation Microglia Activation Mediated Neuroinflammation to Ameliorate Ischemia-reperfusion Injury via STAT5-NF-κB Pathway in Ischemic Stroke

Zhijun Pu, Shengnan Xia, Pengfei Shao, Xinyu Bao, Dan Wu, Yun Xu

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: dauricine; STAT5; NF-κB; Inflammation; Ischemia-reperfusion injury

Online: 8 August 2022 (13:18:00 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202409.1896.v1

MPV17 Prevented Myocardial Ferroptosis and Ischemic Cardiac Injury through Maintaining SLC25A10 Mediated Mitochondrial Glutathione Import

Tao Xu, Guilan Chen

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: ischemia/reperfusion injury; iron overload; myocardial ferroptosis; MPV17; mtGSH

Online: 24 September 2024 (10:49:04 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202410.2553.v1

Protective Effects of Quercetin and Sucrose on Cold-Preservation Injury in Porcine Kidneys Donated After Cardiac Death

Daisuke Ishii, Asuka Toriumi, Hiromichi Obara, Yoshiyasu Satake, Yoko Okada, Haruka Dewa, Masashi Imamura, Yuji Nishikawa, Naoto Matsuno

Subject: Medicine And Pharmacology, Transplantation Keywords: kidney preservation; quercetin; sucrose; cold storage; ischemia-reperfusion injury

Online: 31 October 2024 (12:07:36 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202402.0945.v1

The Effect of Cerium Oxide (CeO2) on Ischemia-Reperfusion Injury in Skeletal Muscle in Mice with Streptozocin-Induced Diabetes

Abdullah Özer, Necmiye Şengel, Ayşegül Küçük, Zeynep Yığman, Çağrı Özdemir, Yiğit Kılıç, Ali Doğan Dursun, Hasan Bostancı, Gülay Kip, Mustafa Arslan

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: diabetes mellitus; cerium oxide; skeletal muscle; lower limb; ischemia-reperfusion

Online: 19 February 2024 (14:12:37 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202305.0309.v1

NCX 470, a Dual Acting Nitric Oxide (NO)-Donating Bimatoprost Derivative, Preserves Rabbit Eyes from Biochemical and Functional Changes Associated with ET-1-Induced Ischemia/Reperfusion Injury

Silvia Sgambellone, Silvia Marri, Serafina Villano, Emanuela Masini, Gustavo Provensi, Elena Bastia, Corinna Galli, Stefania Brambilla, Francesco Impagnatiello, Laura Lucarini

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: nitric oxide; bimatoprost; ischemia/reperfusion; oxidative stress; inflammation; apoptosis

Online: 5 May 2023 (06:00:47 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201708.0015.v1

Omega-3 polyunsaturated fatty acids protect against ischemia-reperfusion renal injury through AMPK-mediated autophagy

Do Hyeong Gwon, Tae Woong Hwang, Ju-ye Ro, Yoon-Joong Kang, Jin Young Jeong, Do-Kyung Kim, Kyu Lim, Dong Woon Kim, Dae Eun Choi, Jwa-Jin Kim

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ischemia-reperfusion injury (IRI), ω3-PUFA, AMP-activated protein kinase (AMPK), autophagy

Online: 4 August 2017 (12:56:08 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202112.0158.v1

Transient Receptor Potential Vanilloid Type 1 Contributes to Modulate the Nitric Oxide Pathway and Oxidative Stress in the Isolated and Perfused Rat Heart during Ischemia and Reperfusion

Vicente Castrejón-Téllez, Leonardo Del Valle-Mondragón, Israel Pérez-Torres, Verónica Guarner-Lans, Gustavo Pastelín-Hernández, Angélica Ruíz-Ramírez, Julieta Anabell Díaz-Juárez, Elvira Varela-López, Victor Hugo Oidor-Chan, Alvaro Vargas-González, Raúl Martínez-Memije, Pedro Flores-Chávez, Bruno León-Ruíz, Sergio Arriaga-Carrillo, Juan Carlos Torres-Narváez

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Ischemia reperfusion; TRPV1; cardioprotection

Online: 9 December 2021 (15:38:27 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201808.0395.v1

Radix Scrophulariae Aqueous Extract Seems to Exert Neuroprotective Effects on Cerebral Ischemia and Reperfusion Injury with Inhibiting Apoptosis via ERK1/2 and p-38 MAPK Pathway

Weijie Xie, Ping Zhou, Xuelian Zhang, Chenyang Zhang, Xiangbao Meng, Guibo Sun, Xiaobo Sun

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: Radix scrophulariae; ischemia stroke; cerebral ischemia and reperfusion; apoptosis; ERK1/2 MAPK; p-38 MAPK

Online: 22 August 2018 (11:07:43 CEST)

Show abstract| Download PDF| Share

Working Paper ARTICLE

Aerobic Exercise Attenuates Myocardial Ischemia-Reperfusion Injury in Rats by Regulating The SIRT3/SOD2/NF-κB Pathway

cailian ruan, Yan Yang, ZiLin Li

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Aerobic exercise; Myocardial ischemia/reperfusion; autophagy; SIRT3; SOD2; NF-κB; Biochemical indicators

Online: 1 April 2021 (13:28:32 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202308.0729.v1

GC-MS Based Cerebrospinal Fluid Metabolomics to Reveal the Protection of Coptisine against Transient Focal Cerebral Ischemia-Reperfusion Injury via Antiinflammation and Antioxidant

Jun Jie Zhang, Ao Qi, Lu lu Liu, Chun Cai, Hui Xu

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Cerebral ischemia-reperfusion; Coptisine; arachidonic acid metabolomics; inflammation; and oxidative stress

Online: 9 August 2023 (08:00:20 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202105.0067.v1

Modulation of Endocannabinoids by Caloric Restriction is Conserved in Mice but is not Required for the Protection from Acute Kidney Injury

K. Johanna R. Hoyer-Allo, Martin Richard Späth, Ruth Hanssen, Marc Johnsen, Susanne Brodesser, Kathrin Kaufmann, Katharina Kiefer, Felix C. Koehler, Heike Göbel, Torsten Kubacki, Franziska Grundmann, Bernhard Schermer, Jens Brüning, Thomas Benzing, Volker Burst, Roman-Ulrich Müller

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: acute kidney injury; ischemia-reperfusion injury; dietary restriction; nutrition; preconditioning; endocannabinoids; AEA

Online: 5 May 2021 (13:59:13 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202410.1322.v1

Restoration of Sestrin 3 Expression Mitigates Cardiac Oxidative Damage in the Ischemia-Reperfusion Injury Model

Mina Park, Sunghye Cho, Dongtak Jeong

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: ischemia– reperfusion injury; Sestrin; miR-25; ROS; oxidative damage; apoptosis; cardiac dysfunction; hypoxia/reoxygenation; antioxidant

Online: 17 October 2024 (11:40:39 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202405.1364.v1

Effects of Sildenafil on the Recovery of Cognitive Function and the Protection of Neuronal Cell Death Following Transient Global Cerebral Ischemia in Gerbils

Yeon Hee Yu, Gun Woo Kim, Yu Ran Lee, Dae-Kyoon Park, Beomjong Song, Duk-Soo Kim

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: global ischemia; reperfusion; sildenafil; behavioral test; neuroprotection

Online: 21 May 2024 (12:49:16 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202401.0975.v1

Cellular Senescence, Mitochondrial Dysfunction, and Their Link to Cardiovascular Disease

Maria Camacho-Encina, Laura K Booth, Rachael Redgrave, Omowumi Folaranmi, Gavin David Richardson

Subject: Biology And Life Sciences, Aging Keywords: mitochondrial dysfunction; senescence; cardiac; cardiomyocyte; cardiac ischemia reperfusion

Online: 12 January 2024 (04:06:44 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202311.1124.v1

Cardioprotective Effect of Epiisopiloturine, an Imidazole Alkaloid from Pilocarpus microphyllus, in Rats Submitted to Cardiac Ischemia and Reperfusion

Pedro Ivo De Marqui Moraes, Fernando Sabia Tallo, Rildo Yamaguti Lima, Sandra Augusta Gordinho Pinto, Erisvaldo Amarante De Araújo, Rodrigo de Barros Freitas, Carolina Trabasso Ferraz De Oliveira, Giovanna Seto De Oliveira, João Vitor Gardelli Trindade, Alexia Nascimento Arias, Rafael Batman de Góes, Carlos Eduardo Braga Filho, Jand Venes Rolim Medeiros, Lucas Antonio Duarte Nicolau, Leiz Maria Costa Véras, Monica Noleto Miranda, Almir Gonçalves Wanderley, Afonso Caricati-Neto, Francisco Sandro Menezes-Rodrigues

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Cardiac Diseases; Cardiac Ischemia-Reperfusion; Myocardial Infarction; Pharmacological Cardioprotection; Epiisopiloloturine

Online: 17 November 2023 (05:22:27 CET)

Show abstract| Download PDF| Share

Working Paper ARTICLE

Association of High Pre-Transplant Caveolin-1 Serum Concentrations with Attenuated Acute Cellular Rejection in Kidney Transplantation

Florian Emmerich, Stefan Zschiedrich, Christine Reichenbach-Braun, Caner Süsal, Susana Minguet, Marie-Christin Pauly, Maximilian Seidl

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Caveolin-1; kidney transplantation; graft rejection; ischemia; reperfusion injury (IRI)

Online: 1 December 2020 (14:08:33 CET)

Show abstract| Download PDF| Supplementary Files| Share

Working Paper ARTICLE

Neuroprotective Effects of Metformin on Cerebral Ischemia-Reperfusion Injury by Regulating PI3K/Akt Pathway

cailian ruan

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Metformin; cerebralischemia-reperfusion; inflammation; PI3K/Akt.

Online: 12 March 2021 (08:43:06 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202309.1325.v1

Pharmacological Modulation of the Ca2+/cAMP/Adenosine Signaling in Cardiac Cells as a New Cardioprotective Strategy to Reduce Severe Arrhythmias in Myocardial Infarction

Fernando Sabia Tallo, Patrícia Oliveira Santana, Sandra Augusta Gordinho Pinto, Rildo Yamaguti Lima, Erisvaldo Amarante De Araújo, José Gustavo Padrão Tavares, Marcelo Pires-Oliveira, Lucas Antonio Duarte Nicolau, Jand Venes Rolim Medeiros, Murched Omar Taha, André Ibrahim David, Bráulio Luna-Filho, Carlos Eduardo Braga Filho, Adriano Henrique Pereira Barbosa, Célia Maria Camelo Silva, Almir Gonçalves Wanderley, Adriano Mendes Caixeta, Afonso Caricat-Neto, Francisco Sandro Menezes-Rodrigues

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: cardiac ischemia-reperfusion; cardiac arrhythmias; pharmacological cardioprotection; Ca2+ channels; adenosine receptors

Online: 20 September 2023 (08:45:21 CEST)

Show abstract| Download PDF| Share

Working Paper REVIEW

Diabetic Cardiomyopathy and Ischemic Coronary Heart Disease: Prevention and Therapy by Exercise and Conditioning

Antonio Crisafulli, Pasquale Pagliaro, Silvana Roberto, Lucia Cugusi, Giuseppe Mercuro, Antigone Lazou, Cristophe Beauloye, Luc Bertrand, Derek Hausenloy, Manuela Aragno, Claudia Penna

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Diabetic Cardiomyopathy; Hyperglycemia; Ischemia/Reperfusion Injury; Metabolism; Mitochondria; Remote Conditioning; Exercise

Online: 17 March 2020 (08:52:25 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202308.0558.v1

Necroptosis in Organ Transplantation: Mechanisms and Potential Therapeutic Targets

Yajin Zhao, Kimberly Main, Tanroop Aujla, Shaf Keshavjee, Mingyao Liu

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: programmed cell death; donor organ condition; ischemia reperfusion injury; allograft rejection; inflammation

Online: 7 August 2023 (12:02:35 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202104.0564.v1

Rapamycin Alternatively Modifies Mitochondrial Dynamics in Dendritic Cells to Reduce Kidney Ischemic Reperfusion Injury

Maria Namwanje, Bijay Bisunke, Thomas V. Rousselle, Gene G. Lamanilao, Venkatadri S. Sunder, Canan Kuscu, Cem Kuscu, Daniel Maluf, Manjari Kiran, Valeria Mas, James D. Eason, Amandeep Bajwa

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Dendritic cell; Rapamycin; Mitochondria; Acute kidney injury; Ischemic Reperfusion Injury

Online: 21 April 2021 (09:13:27 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202208.0352.v1

A Novel Mitophagy Enhancer Protects Cardiomyocytes against Hypoxia/Reoxygenation

Xin Yan, Xiang Gao, Shanshan Hou, Steffen Jockusch, K Michael Gibson, Lanrong Bi

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: Oxidative Stress, Free radical scavenging, Autophagy, Ischemia/reperfusion injury, Hypox-ia/reoxygenation, Mitophagy

Online: 18 August 2022 (14:33:17 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202302.0344.v1

Endothelial Function and Hypoxic-Hyperoxic Preconditioning in Coronary Surgery with a Cardiopulmonary Bypass: Randomized Clinical Trial

Irina A. Mandel, Yuriy K. Podoksenov, Sergey L. Mikheev, Irina V. Suhodolo, Yulia S. Svirko, Vladimir M. Shipulin, Andrey G. Yavorovskiy, Andrey I. Yaroshetskiy

Subject: Medicine And Pharmacology, Anesthesiology And Pain Medicine Keywords: oxygen transport; cardioprotection; hypoxic-hyperoxic preconditioning; ischemia-reperfusion injury; cardiopulmonary bypass; endothelial damage markers

Online: 20 February 2023 (15:14:26 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202103.0770.v1

Role of Citicoline in the Management of Traumatic Brain Injury

Julio J Secades

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: CDP-choline; citicoline; pharmacological neuroprotection; brain ischemia; traumatic brain injury; head injury

Online: 31 March 2021 (14:42:27 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202305.1766.v1

Hypoxic-Ischemic Brain Injury in ECMO: Pathophysiology, Neuromonitoring, and Therapeutic Opportunities

Shivalika Khanduja, Jiah Kim, Jin Kook Kang, Cheng-Yuan Feng, Melissa Ann Vogelsong, Romergryko G Geocadin, Glenn Whitman, Sung-Min Cho

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: hypoxia-ischemia brain injury; extracorporeal membrane oxygenation; neurological complication; neu-romonitoring; outcome.

Online: 25 May 2023 (08:26:02 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202410.2113.v1

Effects of DHEA and DHEAS in Neonatal Hypoxic-Ischemic Brain Injury

Elena Mayer, Ira Winkler, Eva Huber, Martina Urbanek, Ursula Kiechl-Kohlendorfer, Elke Griesmaier, Anna Posod

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: dehydroepiandrosterone; dehydroepiandrosterone sulfate; mouse model; hypoxia-ischemia; neonatal hypoxic-ischemic brain injury; neuroprotection

Online: 28 October 2024 (14:44:16 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202202.0044.v1

Early Effects of Extracellular Vesicles Secreted by Adipose Tissue Mesenchymal Cells in Renal Ischemia Followed by Reperfusion: Mechanisms Rely on the Restoration of the Redox Tissular Environment

Jarlene A Lopes, Federica Collino, Clara Rodrigues-Ferreira, Luzia da Silva Sampaio, Glória Costa-Sarmento, Camila H.C. Wendt, Fernando P. Almeida, Kildare R. Miranda, Tais H. Kasai-Brunswick, Rafael S. Lindoso, Adalberto Vieyra

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: extracellular vesicles; mesenchymal cells; proximal tubular cells; renal ischemia/reperfusion; mitochondria; anion superoxide; acellular therapy; regenerative medicine

Online: 3 February 2022 (10:07:54 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202308.0249.v1

Ultra-High Resolution Electrocardiography Enables Earlier Detection of Transmural and Subendocardial Myocardial Ischemia Compared to Conventional Electrocardiography

Kirill V. Zaichenko, Anna A. Kordyukova, Dmitry L. Sonin, Michael M. Galagudza

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: heart; ischemia; reperfusion; electrocardiography; ultra-high resolution electrocardiography; power spectral density; ischemic heart disease; subclinical atherosclerosis; sensitivity; risk stratification

Online: 3 August 2023 (05:17:42 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202406.1964.v1

Aneurysmal Wall Enhancement of Non-ruptured Intracranial Aneurysms after Endovascular Treatment Correlates with Higher Aneurysm Reperfusion Rates, but Only in Large Aneurysms

Valentin Ladenhauf, Malik Galijasevic, Milovan Regodic, Raimund Helbok, Verena Rass, Christian Freyschlag, Ondra Petr, Johannes Deeg, Leonhard Gruber, Stephanie Mangesius, Elke Ruth Gizewski, Astrid Ellen Grams

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: neuroradiology; aneurysm; reperfusion

Online: 27 June 2024 (15:32:36 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202409.1558.v1

Fenoldopam for Renal Protection in Cardiac Surgery: Pharmacology, Clinical Applications, and Evolving Perspectives

Giuseppe Cuttone, Luigi La Via, Giovanni Misseri, Giulio Geraci, Massimiliano Sorbello, Federico Pappalardo

Subject: Medicine And Pharmacology, Anesthesiology And Pain Medicine Keywords: Acute Kidney Injury; AKI; Renoprotection; Dopamine-1 Receptor Agonist; Ischemia-Reperfusion Injury; Cardiopulmonary Bypass; Renal Blood Flow; Pharmacological Intervention; Perioperative Care

Online: 19 September 2024 (14:52:25 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202306.0567.v1

Stable Coronary Artery Disease: Who Finally Benefits from Coronary Revascularization in the Modern Era? The ISCHEMIA and Interim ISCHEMIA-EXTEND Analysis

Leonid L Bershtein, Alexey Sumin, Elizaveta Zbyshevskaya, Victoria Gumerova, Darejan Tsurtsumia, Igor Kochanov, Alina Andreeva, Vartan Piltakian, Sergey Sayganov

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: chronic coronary syndromes; revascularization; prognosis; ISCHEMIA; ISCHEMIA-EXTEND

Online: 8 June 2023 (02:13:16 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202008.0094.v1

Lessons from Recent Advances in Ischemic Stroke Management and Targeting Kv2.1 for Neuroprotection

Chung-Yang Yeh, Anthony Schulien, Bradley Molyneaux, Elias Aizenman

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Ischemic Stroke; Reperfusion; Neuroprotection; Nerinetide; Kv2.1

Online: 4 August 2020 (11:28:48 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202201.0116.v1

Cardioprotection of Immature Heart by Simultaneous Activation of PKA and EPAC : A Role for the Mitochondrial Permeability Transition Pore

Martin John Lewis, Igor Khaliulin, Katie Hall, M.Saadeh Suleiman

Subject: Medicine And Pharmacology, Anesthesiology And Pain Medicine Keywords: Ischaemia/reperfusion injury; Development; Mitochondria; Immature Heart

Online: 10 January 2022 (13:38:07 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202404.0337.v1

The Role of Tnf and Soluble Tnf Receptors (TNFR1/2) as Therapeutic Targets for Inflammation Secondary to Intracerebral Stroke and Hemorrhage

Rakan Alshaibi, Kanishka Ekanayake, Emily Barlow, Ali Ahmed Mohamed, Brandon Lucke-Wold

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: cerebroprotection; ischemia; tumor necrosis factor

Online: 4 April 2024 (06:04:04 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202203.0301.v1

Acute Heart Failure, 90-day Mortality—and Gravitational Ischemia in the Brain

J. Howard Jaster, Giulia Ottaviani

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Heart Failure; Gravity; Ischemia; Brain

Online: 22 March 2022 (12:38:29 CET)

Show abstract| Download PDF| Share

During the 90 days following hospitalization for acute heart failure, ejection fraction and type of discharge medications have been shown in clinical trials to have little effect on mortality. We examined the recent literature addressing brain-related etiologies of sudden death following heart failure. Two mechanisms of sudden unexpected death have been suggested to possibly result from 4 significant influences on pathophysiology in the brain. The two causes of death are severe cardiac arrhythmia, and neurogenic pulmonary edema. They are both mediated through the brainstem autonomic nuclei generally, and executed specifically through the dorsal motor nucleus of the vagus nerve. The four significant influences on pathophysiology in the brainstem autonomic nuclei are: 1) Hyper-stimulation of neurons in the solitary tract nucleus, increasing their metabolic requirements; 2) Inadequate blood flow at a vascular watershed terminus; 3) Additionally decreased blood flow following vasoconstriction related to relative hyperventilation and decreased pCO2 levels; 4) Gravitational ischemia in the brain—caused by the weight of the brain mass sitting above the brainstem. Changes in head tilt release gravitational ischemia in the brain. There is no specific head position (relative to gravity) that is considered favorable or unfavorable for an extended period of time (i.e. more than 24 hours). Even a small degree of head elevation, used in managing pulmonary congestion, may increase gravitational ischemia in the posterior fossa and brainstem. In this paper we suggest a new and important research avenue for intervening in cardiac arrhythmias, and preventing their occurrence, through the non-invasive use of head-tilting, and other head repositioning maneuvers. This could potentially help many geriatric patients with heart failure, who have decreased mobility in bed, and who tend to stay in one position longer, thereby increasing gravitational ischemia in the brain.

Preprint REVIEW | doi:10.20944/preprints202308.1664.v1

Limb Salvage and Survival in Chronic Limb-Threatening Ischemia: The Need for Fast-Track Team-Based Approach

Giorgio Ventoruzzo, Giulia Mazzitelli, Umberto Ruzzi, Francesco Liistro, Alessia Scatena, Eugenio Martelli

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: peripheral arterial disease; critical limb ischemia; chronic limb-threatening ischemia; survival; mortality; limb salvage

Online: 25 August 2023 (02:41:49 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202304.0094.v1

SUMOtherapeutics for Ischemic Stroke

Paramesh Karandikar, Jakob Gerstl, Ari Kappel, Sae-Yeon Won, Daniel Dubinski, Monica Emili Garcia-Segura, Florian Gessler, Alfred Pokmeng See, Luca Peruzzotti Jametti, Joshua Bernstock

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: stroke; ischemia; neuroprotection; SUMOylation; experimental therapeutics

Online: 6 April 2023 (12:21:33 CEST)

Show abstract| Download PDF| Share

Preprint COMMUNICATION | doi:10.20944/preprints202303.0373.v1

Plasma Galectin-4 Levels are Increased after Stroke in Mice and Humans

Joao Vieira, Amra Jujic, Hana Matuskova, Peter M. Nilsson, Ulf Lindblad, Michael Hecht Olsen, João M.N. Duarte, Anja Meissner, Martin Magnusson

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ischemia; cardiovascular disease; diabetes; obesity; galectins

Online: 21 March 2023 (07:56:47 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints201808.0027.v1

The Hedgehog Signaling Pathway in the Ischemic Heart, Brain, and Skeletal Muscle

Igor Giarretta, Eleonora Gaetani, Paolo Tondi, Takayuki Asahara, Roberto Pola

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Hedgehog; Ischemia; Heart; Brain; Skeletal muscle

Online: 1 August 2018 (16:31:01 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202407.0587.v1

Novel Impact of Colchicine on Interleukin-10 Expression in Acute Myocardial Infarction: An Integrative Approach

Saskia Dyah Handari, Mohammad Saifur Rohman, Djanggan Sargowo, Aulanni’am Aulanni’am, Ricardo Adrian Nugraha, Bayu Lestari, Delvac Oceandy

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Colchicine; IL-10; Acute myocardial infarction; Network pharmacology; Reperfusion

Online: 8 July 2024 (08:35:37 CEST)

Show abstract| Download PDF| Share

Background: Inflammatory plays critical role in myocardial infarction as a critical process in the development of heart failure, involving the development of cardiac fibrosis. Colchicine is a well-established anti-inflammatory drug, but its scientific application in controlling post-acute myocardial infarction remodelling processes has not been established. IL-10, a key cytokine in modulating inflammatory responses, underscoring its potential as a crucial therapeutic target of colchicine. Objectives: To explore the protective role of IL-10 modulated by colchicine in myocardial healing and repair following AMI, particularly cardiac fibrosis. Materials and Methods: The predicted protein of Colchicine was assessed using WAY2DRUG PASS as probability active value. Proteins associated with Colchicine, cardiac fibrosis and acute myocardial infarction were analysed with DisGeNET and Open Target databases. Analysis and visualization of protein-protein interactions were conducted using STRING and Cytoscape. A 3T3 cell line treated with CoCl2 was used to mimic hypoxic. HIF-1α and IL-10 expression were measured by flow cytometry and analysed using a one-way ANOVA test. This observational clinical trial examined acute myocardial infarction patients undergoing immediate and delayed primary percutaneous coronary interventions. Subjects were randomized into control groups receiving placebo and intervention groups treated with Colchicine. Assessments occurred at 24 hours and five days after the intervention. IL-10 expression in the clinical trial was measured by ELISA assay and analysed using a T-test. Results: Colchicine demonstrates promising bioactivity in treating acute myocardial infarction, with notable probability active values as a tubulin antagonist (0.744), beta-tubulin antagonist (0.673), and NOS2 inhibitor (0.529). Its primary action targets IL-10, with protein-protein interactions analysis indicating interactions between IL-10 and key inflammatory mediators—IL-1β, IFN-γ, CCL2, TNF, and TGF-β1—during acute myocardial infarction and cardiac fibrosis. Hypoxic conditions in the CoCl2-induced 3T3 cell model show significantly elevated HIF-1α compared to controls (p<0.0001). Acute myocardial infarction patients were predominantly from high-risk populations, with notable smoking prevalence in the early Colchicine group (72%). Hypertension prevalence was significantly different, with 67.31% in the late Colchicine group compared to 38% in the early Colchicine and 39.80% in the early placebo groups. Both early and late Colchicine groups exhibited a higher prevalence of diabetes mellitus. Colchicine use, either early or late, did not significantly alter major adverse cardiovascular events incidence compared to placebo (p>0.05). However, Colchicine significantly increased IL-10 expression in CoCl2-treated cells (p<0.0001) and in acute myocardial infarction patients within five days (p<0.05). Conclusions: Colchicine may bolster the anti-inflammatory response post-myocardial infarction by activating IL-10 pathways in fibroblasts and in clinical settings, potentially reducing major adverse cardiovascular events. Further investigation into broader aspects of this pathway, particularly in cardiac fibroblasts, is required.

Preprint ARTICLE | doi:10.20944/preprints202402.1027.v1

TRIM2 Selectively Regulates Inflammation-Driven Pathological Angiogenesis without Affecting Physiological Hypoxia-Mediated Angiogenesis

Nathan K.P. Wong, Emma L. Solly, Richard Le, Victoria A. Nankivell, Jocelyne Mulangala, Peter J. Psaltis, Stephen J. Nicholls, Martin K.C. Ng, Christina A. Bursill, Joanne T.M. Tan

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Inflammation; Ischemia; Neovascularization; HIF-1α ; eNOS

Online: 19 February 2024 (08:33:17 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint CASE REPORT | doi:10.20944/preprints202311.1336.v1

Pneumatosis Intestinalis Concurrent with Superior Mesenteric Artery Thrombosis: A Case Report

Parihar Doshi, Tushar Teraiya, Vismit Gami, Sahil Shah, Dev Desai

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: Pneumatosis intestinalis; Thrombosis; CT Scan; Atherosclerosis; Ischemia

Online: 21 November 2023 (13:43:57 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202311.0909.v1

A Review of the Scope of Wearable Ultrasonic Sensors for Ischemia Monitoring

Evan Brophy, Masudul Imtiaz

Subject: Engineering, Electrical And Electronic Engineering Keywords: Blood Pressure; Ischemia Monitoring; Wearable Ultrasonic Sensor

Online: 15 November 2023 (03:51:00 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202102.0289.v1

Post Stroke Depression to be Expected

Tissa Wijeratne, Carmela Sales

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cytokine, Depression, Ischemia, Stroke, Apoptosis, Excitotoxicity, Onecosis, Inflammation,

Online: 11 February 2021 (16:46:06 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202409.0549.v1

Methodological Approaches to Experimental Evaluation of Neuroprotective Action of Potential Drugs

Igor Belenichev, Nina Bukhtiyarova, Victor Ryzhenko, Lyudmyla Makyeyeva, Oksana Morozova, Valentyn Oksenych, Oleksandr Kamyshnyi

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: ischemic stroke; models of cerebral ischemia; neuroprotection; drug effect

Online: 6 September 2024 (16:47:26 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202404.0575.v1

Cannabinoids and Cardiac Rhythms: Unveiling the Relationship between Cannabis Use, ECG Changes, and Arrhythmias

Behzad Fahimi, Somayeh Beikmohammadi, Parsa Rostami

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Cannabis; Electrocardiogram (ECG); Arrhythmias; Myocardial ischemia; Cardiac ion channels

Online: 8 April 2024 (15:02:42 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202402.1406.v1

Could Phosphorous MR Spectroscopy Help Predict the Severity of Vasospasm? A Pilot Study

Malik Galijasevic, Ruth Steiger, Stephanie Alice Treichl, Wing Man Ho, Stephanie Mangesius, Valentin Ladenhauf, Johannes Deeg, Leonhard Gruber, Miar Ouaret, Milovan Regodic, Lukas Lenhart, Bettina Pfausler, Astrid Ellen Grams, Ondra Petr, Claudius Thomé, Elke Ruth Gizewski

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: subarachnoidal hemorrhage, brain aneurysm, phosphorous spectroscopy, vasospasm, delayed ischemia

Online: 26 February 2024 (09:42:36 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202212.0152.v1

Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Massimo Sangiorgi, Mariangela Valentina Puci, Allegra Rosa Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia Di Pinto, Franco Michelino Fiore, Armado Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massara, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Pietro Modugno, Maurizio Maiorano, Umberto Marcello Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni Maria Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaó, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio Stefano Tolva, Ilenia D'Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D'Arrigo, Giusi Basile, Dalmazio Frigerio, Gian Franco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio Massimo Oddi, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: chronic limb-threatening ischemia; outcome; sex; age; limb salvage

Online: 8 December 2022 (09:39:30 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202104.0205.v1

Different Lower Extremity Arterial Calcification Patterns in Patients with Chronic Limb-Threatening Ischemia Compared with Asymptomatic Controls

Louise CD Konijn, Richard AP Takx, Willem PThM Mali, Hugo TC Veger, Hendrik van Overhagen

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: chronic limb-threatening ischemia; peripheral arterial disease; calcification pattern

Online: 7 April 2021 (14:45:17 CEST)

Show abstract| Download PDF| Share

Objectives The most severe type of peripheral arterial disease (PAD) is critical limb ischaemia (CLI). In CLI, calcification of the vessel wall plays an important role in symptoms, amputation rate and mortality. However, calcified arteries are also found in asymptomatic persons (non-PAD patients). We investigated whether the calcification pattern in CLI patients and non- PAD patients are different and could possibly explain the symptoms in CLI patients. Materials and Methods 130 CLI and 204 non-PAD patients underwent a CT of the lower extremities. This resulted in 118 CLI patients (mean age 72±12, 70.3% male) that were age-matched with 118 non-PAD patients (mean age 71±11, 51.7% male). The characteristics severity, annularity, thickness and continuity were assessed in the femoral and crural arteries and analysed by binary multiple logistic regression. Results Nearly all CLI patients have calcifications and these are equally frequent in the femoropopliteal (98.3%) and crural arteries (97.5%), while the non-PAD patients had in just 67% any calcifications with more calcifications in the femoropopliteal (70.3%) than in the crural arteries (55.9%, p<0.005). The crural arteries of the CLI patients had significantly more complete annular calcifications (OR 2.92, p=0.001.) while in the non-PAD patients dot-like calcifications dominated. In CLI patients, the femoropopliteal arteries had more severe, irregular / patchy and thick calcifications (OR 2.40, 3.27, 1.81, p≤0.05, respectively) while in non-PAD patients, thin continuous calcifications prevailed. Conclusions Compared with non-PAD patients CLI patients are more frequently and extensively calcified. Annular calcifications were found in the crural arteries of CLI patients while dot-like calcifications were mostly present in the non-PAD patients. These different patterns of calcifications in CLI point at different etiology and can have prognostic and eventually therapeutic consequences.

Preprint ARTICLE | doi:10.20944/preprints202407.1979.v1

The Impact of Gel Parameters on the Dispersal and Fragmentation of Hyaluronic Acid Gel Fillers Within an Artificial Model of Arterial Embolism

Danny J Soares, Alec D McCarthy

Subject: Medicine And Pharmacology, Other Keywords: vascular occlusion; arterial embolism; dermal filler injuries; soft tissue ischemia

Online: 25 July 2024 (11:04:01 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202406.1171.v1

The Correlations Between Kansas City Cardiomyopathy Questionnaire and Electrocardiographic Parameters in Predicting the Outcome after Cardiac Resynchronization Therapy

Andrei-Mihnea Rosu, Luminiţa Florentina Tomescu, Theodor-Georgian Badea, Emanuel-Stefan Radu, Andreea-Liana Rosu, Lavinia Nicoleta Brezeanu, Adelina Vlad, Maria-Daniela Tanasescu, Andrada-Georgiana Badea, Sebastian Isac, Teodora Isac, CRINA SINESCU, Roxana Darabont, Catalina-Liliana Andrei, Tudorel Andrei, Oana Andreea Popa

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Kansas city cardiomyopathy questionnaire; cardiac resynchronization therapy; electrocardiography; cardiac ischemia

Online: 17 June 2024 (15:51:30 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202405.0860.v1

Diagnostic Role of MDCT in Acute Mesenteric Ischemia

Francesco Michele Ronza, Teresa Letizia Di Gennaro, Gianfranco Buzzo, Luciana Piccolo, Marina Della Noce, Giovanni Giordano, Giuseppe Posillico, Francesco Giuseppe Mazzei, Paolo Ricci, Salvatore Masala, Mariano Scaglione, Stefania Tamburrini

Subject: Medicine And Pharmacology, Emergency Medicine Keywords: acute mesenteric ischemia; MDCT; atherosclerosis; embolism; dissection; NOMI; mesenteric thrombosis

Online: 13 May 2024 (14:31:46 CEST)

Show abstract| Download PDF| Share

Preprint CASE REPORT | doi:10.20944/preprints202401.1796.v1

Retrograde Endovascular Recanalization of the Superior Mesenteric Artery for the Treatment of Acute Bowel Ischemia: Case Report

Pawel Latacz, Piotr Piekorz, Marian Simka

Subject: Medicine And Pharmacology, Emergency Medicine Keywords: acute bowel ischemia; mesenteric artery occlusion; mesenteric artery stenting; retrograde

Online: 25 January 2024 (07:12:18 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202401.0774.v1

Myocardial Perfusion Imaging and C-reactive Protein in Myo-Cardial Ischemia: A Retrospective Single-Center Study

Aidonis Rammos, Aris Bechlioulis, Areti Kekiopoulou, Pavlos Kekiopoulos, Christos S. Katsouras, Chrissa Sioka

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: C-reactive protein; ischemia; SPECT; myocardial perfusion imaging; cardiac imaging

Online: 10 January 2024 (10:08:28 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202312.1073.v1

Prospects of Intravenous CoenzymeQ10 Administration in Emergency Ischemic Conditions

Elena Igorevna Kalenikova, Evgeniya Aronovna Gorodetskaya, Oxana Viktorovna Povarova, Oleg Stefanovich Medvedev

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: coenzyme Q10; ubiquinol; ubiquinone; brain ischemia; myocardial infarct; intravenous administration

Online: 14 December 2023 (09:10:35 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202308.0327.v1

The Interplay between Mitochondrial dysfunction and Ferroptosis during Ischemia-associated Central Nervous System Diseases

Heyan Tian, Boyang Huang, Huifang Nie, Xiangyu Chen, Yue Zhou, Tong Yang, Shaowu Cheng, Zhigang Mei, Jinwen Ge

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: cerebral ischemia; central nervous system diseases; mitochondrial dysfunction, ferroptosis; therapeutics

Online: 4 August 2023 (11:02:35 CEST)

Show abstract| Download PDF| Share

Cerebral ischemia, which is a leading cause of disability and mortality worldwide, sets off a chain of molecular and cellular pathologies that are associated with some central nervous system (CNS) disorders, mainly including ischemic stroke, Alzheimer's disease (AD), Parkinson's disease (PD), epilepsy and other CNS diseases. In recent times, despite significant advancements in the treatment of the pathological processes underlying various neurological illnesses, effective therapeutic approaches specifically targeted at minimizing the damage of such diseases remain absent. Remarkably, ischemia causes severe damage to cells in Ischemia-associated CNS Diseases. Cerebral ischemia initiates oxygen and glucose deprivation, which subsequently promotes mitochondrial dysfunction, including MPTP opening, mitophagy dysfunction, and excessive mitochondrial fission, triggering various forms of cell death, such as autophagy, apoptosis, as well as ferroptosis. Ferroptosis, a novel type of regulated cell death (RCD), is characterized by iron-dependent accumulation of lethal reactive oxygen species and lipid peroxidation. Mitochondrial dysfunction and ferroptosis both play critical roles in the pathogenic progression of Ischemia-associated CNS Diseases. In recent years, growing evidence has indicated that mitochondrial dysfunction interplays with ferroptosis to aggravate cerebral ischemia injury. However, the potential connections between mitochondrial dysfunction and ferroptosis in Cerebral Ischemia have not yet been clarified. Thus, we analyze the underlying mechanism between mitochondrial dysfunction and ferroptosis in Ischemia-associated CNS Diseases. We also discovered that GSH depletion and GPX4 inactivation cause lipoxygenase activation and calcium influx following cerebral ischemia injury, resulting in MPTP opening and mitochondrial dysfunction. Additionally, dysfunction in mitochondrial electron transport and an imbalanced fusion-to-fission ratio can lead to the accumulation of reactive oxygen species and iron overload, which further contribute to the occurrence of ferroptosis. This creates a vicious cycle that continuously worsens cerebral ischemia injury. In this study, our focus is on exploring the interplay between mitochondrial dysfunction and ferroptosis, which may offer new insights into potential therapeutic approaches for the treatment of Ischemia-associated CNS Diseases.

Preprint REVIEW | doi:10.20944/preprints202306.2174.v1

The Effect of Andrographis paniculata and Its Derivatives in Neurodegenerative Diseases and Neuroinflammation

Francesca Bosco, Stefano Ruga, Rita Citraro, Antonio Leo, Lorenza Guarnieri, Jessica Maiuolo, Francesca Oppedisano, Roberta Macrì, Federica Scarano, Saverio Nucera, Irene Bava, Ernesto Palma, Carolina Muscoli, Juan Hancke, Giovambattista De Sarro, Vincenzo Mollace

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Andrographis paniculata; Andrographolide; Neurodegenerative Diseases; Alzheimer; Parkinson; Brain Ischemia; Neuroinflammation

Online: 30 June 2023 (07:38:39 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202201.0065.v1

Disassembly and Mislocalization of AQP4 in Incipient Scar Formation After Experimental Stroke

Shervin Banitalebi, Nadia Skauli, Samuel Geiseler, Ole Petter Ottersen, Mahmood Amiry-Moghaddam

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Aquaporin-4; AQP4ex; Stroke; Ischemia; Astrocyte; Astrogliosis; Glial scar; Neuroinflammation

Online: 6 January 2022 (10:16:02 CET)

Show abstract| Download PDF| Supplementary Files| Share

Working Paper ARTICLE

Local Anesthetic Delivered With a Dual Action Ring and Injection Applicator Numnuts® Reduces the Acute Pain Response of Lambs During Tail Docking

Alison Small, Danila Marini, Ian Colditz

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: analgesia; sheep; ischemia; latex ring; rubber ring; de-tailing; behavior.

Online: 1 June 2021 (09:50:27 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201806.0381.v1

CLINICAL PROFILE OF PATIENTS WITH CORONARY TORTUOSITY AND ITS RELATION WITH CORONARY ARTERY DISEASE

Ankush Gupta, Prashant Panda, YASH SHARMA, Ashwin Mahesh, Prafull Sharma, Nalin Kumar Mahesh

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: coronary tortuosity; myocardial ischemia; coronary artery disease; chronic stable angina.

Online: 25 June 2018 (11:02:03 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202405.0460.v1

Macular Ischemia Changes in Patients with Diabetic Macular Edema Treated with Aflibercept and Ranibizumab

Dimitrios Maris, Anna Dastiridou, Maria Kotoula, Aikaterini Karathanou, Evangelia E. Tsironi, Alexandra Bargiota, Sofia Androudi

Subject: Medicine And Pharmacology, Ophthalmology Keywords: diabetic macular edema; ranibizumab; aflibercept; ischemia; fovea avascular zone; OCT angiography

Online: 8 May 2024 (10:35:00 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202407.2243.v1

Non-Obstructive Coronary Artery Disease and Atrial Fibrillation: A Complex Interplay with One Begets Another

Alexandru-Florinel Oancea, Paula Cristina Morariu, Ana Maria Buburuz, Ionela-Larisa Miftode, Radu Stefan Miftode, Ovidiu Mitu, Alexandru Jigoranu, Diana-Elena Floria, Amalia Timpau, Andrei Vata, Claudia Plesca, Gina Botnariu, Alexandru Burlacu, Dragos -Viorel Scripcariu, Mitea Raluca, Magdalena Cuciureanu, Daniela Maria Tanase, Irina Iuliana Costache-Enache, Mariana Floria

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: coronary ischemia; non-obstructive coronary artery disease; INOCA; MINOCA; ANOCA; atrial fibrillation

Online: 30 July 2024 (10:00:57 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.1523.v1

Topical Application of Autologous Leukocyte-Rich Platelet-Rich Plasma with a High Concentration of Monocytes in Patients with Recalcitrant Ischemic Diabetic Foot Ulcers: Efficacy, Safety and Economic Evaluation.

Benedetta Ragghianti, Giovanni Antonio Silverii, Bianca Maria Berardi, Linda Luppichini, Maria Masulli, Matteo Monami

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Diabetes mellitus; Foot ulcer; Cell-therapy; Chronic Limb-Threatening Ischemia; Economic evaluation

Online: 20 December 2023 (10:18:08 CET)

Show abstract| Download PDF| Supplementary Files| Share

BACKGROUND: Patients with diabetic foot ulcers (DFU) and chronic limb-threatening ischemia (CLTI are at higher risk for major amputations and mortality. The aim of this study was to assess the safety and cost-efficacy of intramuscular injection of autologous monocyte-rich PRP platelet-rich plasma Tropocells® PRP (Estar Medical, Holon, Israel) in diabetic patients with chronic limb-threatening ischemia (CLTI) in which revascularization was not feasible. METHODS: a retrospective study was carried out on a series of patients with type 2 diabetes, grade Texas 3C or D DFU, and CLTI. All patients had undergone at least a previous revascularization and were allocated to a surgery waiting list for major amputation. According to the data provided by the manufacturer (https://www.zuccatobiotech.it/index.php/prodotti/tropocells), the monocyte-rich PRP kit obtained a platelet-rich plasma with a high concentration of peripheral blood mononuclear cells (with a proportionately lower amount of lymphocytes), a low concentration of neutrophils and an almost complete depletion of red blood cells. All patients underwent a procedure of local infiltration of autologous mononuclear cells through multiple perilesional and intramuscular injections of 10 mL PBMNC suspensions (0.2–0.3 mL in boluses) performed below the knee along the relevant vascular axis (anterior tibial artery and posterior tibial artery) at intervals of 1–2 cm and to a mean depth of 1.5–2 cm, using a 21 G needle. The procedures were performed according to the instructions of the manufacturer and were repeated at least two times (maximum 3 times) at intervals of 30 days. The principal endpoint was a composite of TcPO2 values at the first toe ≥30 mmHg and/or TcPO2 increase of at least 50% from baseline and/or ulcer healing. Secondary endpoints were individual components of the primary endpoint, quality of life, any adverse events, and direct costs. All the endpoints were evaluated at 26 weeks. RESULTS: Out of 21 enrolled patients, 13 (61.9%) reached the primary endpoint, whereas three patients underwent a major amputation, and two patients died. The median TpCO2 was significantly increased, and pain was significantly reduced. The overall mean and median cost per patient were 9,255±7,328€ and 4,001 [2,991;7,565]€, respectively. CONCLUSIONS: the use of PBMNCs implants in patients with DFU and no-option CLTI seems to be efficacious in reducing the risk of major amputation and improve ulcer healing.

Preprint REVIEW | doi:10.20944/preprints202312.0244.v1

Translational Relevance of Advanced Age and Atherosclerosis in Preclinical Trials of Biotherapies for Peripheral Artery Disease

Keith A Webster

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: gene therapy; cell therapy; peripheral artery disease; critical limb ischemia; clinical trials

Online: 5 December 2023 (14:44:09 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202205.0184.v1

Amyloid Precursor Protein, Alpha-, Beta- and Gamma-Secretases Expression in Penumbra Cells after Photothrombotic Stroke. Evaluation of the Neuroprotective Effect of Secretase Inhibitors

Svetlana Sharifulina, Valeria Guzenko, Alexandr Logvinov, Andrey Khaitin, Yuliya Kalyuzhnaya, Natalia Dobaeva, Yan Li, Lei Chen, Вin He, Svetlana Demyanenko

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloid precursor protein; photothrombotic stroke; ischemia; alpha-secretase; beta-secretase; gamma-secretase

Online: 13 May 2022 (08:39:09 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Working Paper REVIEW

HypoxiaInducible Factor (HIF) in Ischemic Stroke and Neurodegenerative Disease

Elena Mitroshina, Maria Savyuk, Evgeni Ponimaskin, Maria Vedunova

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Hypoxia Inducible Factor; HIF; Ischemia; Hypoxia; Adaptation; Alzheimer’s Disease; Parkinson Disease; Neurodegeneration

Online: 26 February 2021 (15:34:31 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202309.0742.v1

A Network Comprised of miR-15b and miR-29a is Involved in VEGFA Pathway Regulation in Thymus Adipose Tissue from Elderly Ischemic Cardiomyopathy Subjects

Adriana-Mariel Gentile, Said Lhamyani, Maria Mengual Mesa, Francisco Javier Pavón-Morón, John R Pearson, Julian Salas, Mercedes Clemente-Postigo, Lucia Perez Costillas, Gabriel Olveira, Rajaa El Bekay

Subject: Biology And Life Sciences, Aging Keywords: aging; angiogenesis; elderly; ischemia; microRNA; myocardial infarction; thymus adipose tissue; subcutaneous adipose tissue

Online: 12 September 2023 (07:09:18 CEST)

Show abstract| Download PDF| Supplementary Files| Share

Preprint REVIEW | doi:10.20944/preprints202307.1219.v1

Immunomodulatory and Antioxidant Drugs in Glaucoma Treatment

Francesco Buonfiglio, Norbert Pfeiffer, Adrian Gericke

Subject: Medicine And Pharmacology, Ophthalmology Keywords: autoimmune; glaucoma; retinal ganglion cell; optic nerve; inflammation; ischemia; oxidative stress; vascular dysregulation

Online: 18 July 2023 (11:37:09 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints201812.0128.v1

Novel Role of MCP-Induced Protein 1 in Ischemic Brain Damage in Animals of Transient Focal Ischemia

Zhuqing Jin, Jian Liang, Jiaqi Li, Pappachan E. Kolattukudy

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: middle cerebral artery occlusion (MCAO); MCPIP1; blood-brain barrier; inflammatory response; ischemia; metalloproteinases

Online: 11 December 2018 (10:49:54 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202309.1715.v1

Percutaneous Deep Venous Arterialization for Limb Salvage of No Option Patients with Chronic Limb-Threatening Ischemia

Stavros Spiliopoulos, Efstathia Davoutis, Nikolaos-Achilleas Arkoudis, Kaji Sritharan, Symeon Lechareas

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: chronic limb-threatening ischemia; peripheral arterial disease; endovascular treatment; tibial vessels; percutaneous deep venous arterialization

Online: 26 September 2023 (04:44:37 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202305.1171.v1

Topical Application of Autologous Peripheral Blood Mononuclear Cells in Patients with Small Artery Disease and Diabetic Foot Ulcers: Efficacy, Safety and Economic Evaluation

Benedetta Ragghianti, Bianca Maria Berardi, Edoardo Mannucci, Matteo Monami

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Diabetes mellitus; Foot ulcer; Cell-therapy; Small Artery Disease; Chronic Limb-Threatening Ischemia; Economic evaluation

Online: 17 May 2023 (02:41:10 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202206.0230.v1

How the First Year of the COVID-19 Pandemic Impacted Patients’ Care in the Vascular Surgery Divisions of the Southern Regions of the Italian Peninsula

Eugenio Martelli, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Sangiorgi, Matilde Zamboni, Allegra Rosa Martelli, Giancarlo Accarino, Giuseppe Bianco, Francesco Bonanno, Umberto Marcello Bracale, Enrico Cappello, Giovanni Cioffi, Giovanni Colacchio, Adolfo Crinisio, Salvatore De Vivo, Carlo Patrizio Dionisi, Loris Flora, Giovanni Impedovo, Francesco Intrieri, Luca Iorio, Gabriele Maritati, Piero Modugno, Mario Monaco, Giuseppe Natalicchio, Vincenzo Palazzo, Fernando Petrosino, Francesco Pompeo, Raffaele Pulli, Davide Razzano, Maurizio Ruggero Ruggieri, Carlo Ruotolo, Paolo Sangiuolo, Gennaro Vigliotti, Pietro Volpe, Antonella Biello, Pietro Boggia, Michelangelo Boschetti, Enrico Maria Centritto, Flavia Condò, Lucia Cucciolillo, Amodio Salvatore D'Amodio, Mario De Laurentis, Claudio Desantis, Daniela Di Lella, Giovanni Di Nardo, Angelo Disabato, Ilaria Ficarelli, Angelo Gasparre, Antonio Nicola Giordano, Alessandro Luongo, Mafalda Massara, Vincenzo Molinari, Andrea Padricelli, Marco Panagrosso, Anna Petrone, Serena Pisanello, Roberto Prunella, Michele Tedesco, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: COVID-19; carotid stenosis; abdominal aortic aneurysm; chronic limb-threatening ischemia; amputation; deep venous thrombosis

Online: 16 June 2022 (04:40:54 CEST)

Show abstract| Download PDF| Share

Preprint CASE REPORT | doi:10.20944/preprints202406.1488.v1

Partial Reversal of Coronary Stenosis with Intravenous Hydrogen and Oxygen Nanobubble Administration: A Case Report

Aditya T. Hernowo, James C. Pardosi, S. Subagjo, Aning Tyas K. Dewi, Sutiman B. Sumitro

Subject: Medicine And Pharmacology, Complementary And Alternative Medicine Keywords: coronary artery disease; stenosis; nanobubble; hydrogen; oxygen; antioxidant; ultrafine bubble; nanomedicine; anti‐inflammatory; cardiovascular; ischemia; atherosclerosis

Online: 21 June 2024 (07:24:22 CEST)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202011.0193.v1

Current Status of Cell-Based Therapy in Patients with Critical Limb Ischemia

Frantisek Jaluvka, Peter Ihnat, Juraj Madaric, Vaclav Prochazka

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Cell therapy, Peripheral arterial disease, Bone marrow, Adipose tissue, Mesenchymal stem cells, Exosome, Critical limb ischemia.

Online: 4 November 2020 (12:37:47 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202104.0240.v1

Considerations to Model Heart Disease in Women with Preeclampsia and Cardiovascular Disease

Clara Liu Chung Ming, Kimberly Sesperez, Eitan Ben-Sefer, David Arpon, Kristine McGrath, Lana McClements, Carmine Gentile

Subject: Engineering, Automotive Engineering Keywords: preeclampsia; cardiovascular disease; heart failure; ischemic/reperfusion injury; in vivo model system; in vitro model system; ex vivo model system

Online: 8 April 2021 (12:23:43 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202407.1121.v1

Effects on Oxidative Stress of Oncological Surgery, Assessed by Plasma Xanthine Oxidase and Adenosine Deaminase Activity

Bruno Pastene, Marion Marlinge, Julien Fromonot, Julia Dodivers, Amin Ben Lassoued, Nathalie Lalevée, Pascal-Alexandre Thomas, David Jérémie Birnbaum, Marc Leone, Laurent Zieleskiewicz, Régis Guieu

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: solid tumor cancers; oxidative stress; adenosine deaminase; xanthine oxidase; ischemia modified albumin; redox potential; major surgery; anesthesia

Online: 16 July 2024 (04:49:59 CEST)

Show abstract| Download PDF| Share

In solid cancer surgery, oxidative stress (OS) is of particular interest because both the malignancy itself and the surgical intervention are major contributors to the modulation of OS levels. This study aimed to investigate the effects of solid cancer surgery on OS levels. Because solid cancer is often accompanied by intense cell death, the study specifically focused on the pathway leading to uric acid formation, (i.e. adenosine deaminase (ADA) and xanthine oxidase (XO). Ische-mia-modified albumin (IMA), a recognized marker of oxidative stress, and plasma redox poten-tial (RP) were also measured. Thirty-six patients undergoing major solid cancer surgery (median age 68 years (58-76), 23 men and 13 women) and thirty controls (62 years (51-80)) were included. During the surgical procedure, serum ADA levels did not change significantly (10.4 International Units (IU) (6.9 – 13.7) vs. 8 IU (6 – 12); p = 0.47). In contrast, XO (IU), IMA (Arbitrary Units(AU)) and redox potential (mV) decreased significantly (22.5 IU (11 – 63) vs. 8.4 IU (5 – 27); p = 0.0041, 89 AU (69 – 105) vs. 74 AU (66 – 80); p = 0.01 and 98 mV (78 – 118) vs. 79 mV (65 – 101); p = 0.033). The correlation between XO and IMA or redox potential was positive (Spearman’s r = 0.32 ; p = 0.007, and r = 0.58; p < 0.001, respectively). XO was negatively correlated with ADA (r = -0.26; p = 0.03). Redox potential was positively correlated with IMA (r = 0.26; p = 0.04) and nega-tively correlated with ADA (r = -0.25 ;p = 0.04). We found that a major source of OS in solid cancer could be the result of an increase in xanthine oxidase activity and that the global effect of solid cancer surgery is a significant decrease in OS levels. We also found that ADA and XO were inversely correlate, suggesting the existence of a negative retrocontrol between the production of free radicals and the production of their sub-strate. Finally, our study highlights the potential value of XO inhibitors as adjuvant therapy in solid tumor surgery.

Preprint ARTICLE | doi:10.20944/preprints202206.0214.v1

Theory and Applications of the (Cardio) Genomic Fabric Approach to Post-Ischemic and Hypoxia-Induced Heart Failure

Dumitru Andrei Andrei Iacobas, Lei Xi

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Adra1b; cardiac ischemia; hypoxia; Crem; gene expression control; gene expression coordination; gene hierarchy; heart failure; transcriptomic stoichiometry

Online: 15 June 2022 (07:37:53 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202312.1268.v1

Prevention of Delayed Graft Function in Kidney Transplant Recipients by Continuous Infusion of Prostaglandin-Analogue Iloprost: A Single-Center Prospective Study

Massimiliano Veroux*, Floriana Sanfilippo, Giuseppe Roscitano, Martina Maria Giambra, Alessia Giaquinta, Giordana Riccioli, Domenico Zerbo, Daniela Corona, Massimiliano Sorbello, Pierfrancesco Veroux

Subject: Medicine And Pharmacology, Transplantation Keywords: Kidney transplantation; Marginal Donor; Cold Ischemia Time; Donor Age; Acute kidney Injury; Deceased Donor; Graft Survival; Patient Survival

Online: 18 December 2023 (06:07:16 CET)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202102.0384.v1

Prospects of Intraoperative Multimodal OCT Application in Patients With Acute Mesenteric Ischemia

Elena Kiseleva, Maxim Ryabkov, Mikhail Baleev, Evgeniya Bederina, Pavel Shilyagin, Vladimir Beschastnov, Ivan Romanov, Grigory Gelikonov, Natalia Gladkova

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: acute mesenteric ischemia (AMI); optical coherence tomography (OCT); optical coherence angiography (OCA); intraoperative diagnostics; gut vitality; image assessment

Online: 17 February 2021 (12:02:23 CET)

Show abstract| Download PDF| Share

Introduction: Despite the introduction of increasingly multifaceted diagnostic techniques and the general progress of emergency abdominal and vascular surgery, the outcome of treatment of patients with acute impaired intestinal circulation remains unsatisfactory. The non-invasive and high-resolution technique of optical coherence tomography (OCT) can be used intraoperatively to assess intestine viability and associated conditions that frequently emerge under conditions of impaired blood circulation. This study aims to demonstrate the effectiveness of multimodal (MM) OCT for intraoperative diagnostics of both the microstructure (cross-polarization OCT mode) and microcirculation (OCT angiography mode) of the small intestine wall in patients with acute mesenteric ischemia (AMI). Methods and Participants: A total of 18 patients were enrolled in the study. Nine of them suffered from AMI in segments II-III of the superior mesenteric artery (AMI group), where the ischemic segments of the intestine were examined. Nine others were operated for adenocarcinoma of the colon (control group), thus allowing areas of their normal small intestine to be examined for comparison. Data on the microstructure and microcirculation in the walls of the small intestine were obtained intraoperatively from the side of the serous membrane using the MM OCT system (IAP RAS, Russia) before bowel resection. The MM OCT data were compared with the results of histological examination. Results: The study finds that MM OCT visualized the damage to serosa, muscularis externa, and blood vessels localized in these layers in 100% of AMI cases. It also visualized the submucosa in 33.3% of AMI cases. The MM OCT image of non-ischemic (control group), viable ischemic and necrotic small intestines (AMI group) differed significantly across stratification of the distinguishable layers, the severity of intermuscular fluid accumulations and the type and density of the vasculature. Conclusion: The MM OCT diagnostic procedure optimally meets the requirements of emergency surgery. Data on the microstructure and microcirculation of the intestinal wall can be obtained simultaneously in real time without requiring contrast agent injections. The depth of visualization of the intestinal wall from the side of the serous membrane is sufficient to assess the volume of the affected tissues. However, the methodology for obtaining MM OCT data needs to be improved to minimize the motion artefacts generated in actual clinical conditions.

Preprint REVIEW | doi:10.20944/preprints202310.1240.v1

Advancements in Treatment Strategies for Chronic Mesenteric Ischemia: A Comprehensive Review

Genti Xhepa, Andrea Vanzulli, Lucilla Violetta Sciacqua, Agostino Inzerillo, Pierre Faerber, Anna Maria Ierardi, Gianpaolo Carrafiello, Filippo Del Grande, Alexis Ricoeur

Subject: Medicine And Pharmacology, Surgery Keywords: review; chronic mesenteric ischemia; superior mesenteric artery; revascularization; treatment; endovascular revascularization (ER); percutaneous transluminal angioplasty (PTA); primary stenting (PMAS)

Online: 19 October 2023 (10:14:50 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202406.1966.v1

Rasagiline Confers Neuroprotection to PC12 Cell Cultures Exposed to Oxygen-Glucose Deprivation/Reoxygenation Insult by Activating the Akt/Nrf2 Redox-Signaling Pathway and by Decreasing the Nuclear Translocation of Glyceraldehyde-3-Phosphate Dehydroge

Shimon Lecht, Adi Lahiani, Michal Klazas, Majdi Saleem Naamneh, Limor Rubin, Jiayi Dong, Wenhua Zheng, Philip Lazarovici

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Akt; cell death; GAPDH; ischemia-like insult; neuroprotection; Nrf2; phosphorylation; Rasagiline; ROS; PC 12 neuronal model; α-synuclein; Parkinson’s disease; stroke

Online: 1 July 2024 (08:40:53 CEST)

Show abstract| Download PDF| Share

Preprint COMMUNICATION | doi:10.20944/preprints202403.0753.v1

Noninvasive Ambulatory Electrocardiographic Markers from Patients with COVID-19 Pneumonia: A Report of Three Cases

Motohiro Kimata, Kenichi Hashimoto, Naomi Harada, Yusuke Kawamura, Yoshifumi Kimizuka, Yuji Fujikura, Mayuko Kaneko, Nobuaki Kiriu, Yasumasa Sekine, Natsumi Iwabuchi, Tetsuro Kiyozumi, Akihiko Kawana, Susumu Matukuma, Yuji Tanaka

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: ambulatory electrocardiographic markers; coronavirus disease-19; COVID-19; global cardiac ischemia; Holter electrocardiograph; myocardial remodeling; SARS-CoV-2; sudden cardiac death

Online: 13 March 2024 (07:31:31 CET)

Show abstract| Download PDF| Supplementary Files| Share

Preprint ARTICLE | doi:10.20944/preprints202309.2168.v1

Alterations in the amyloid protein precursor, α-secretase, β-secretase, presenilins and tau protein Genes in the CA3 Area of the Hippocampus in a 2-Year Ischemic Model of Alzheimer’s Disease

Stanisław Czuczwar, Janusz Kocki, Barbara Miziak, Jacek Bogucki, Anna Bogucka-Kocka, Ryszard Pluta

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: brain ischemia; Alzheimer’s disease; CA3 area; amyloid protein precursor; amyloid; α-secretase; β-secretase; presenilin 1 and 2; tau protein; genes

Online: 30 September 2023 (08:01:45 CEST)

Show abstract| Download PDF| Share

Understanding the phenomena underlying the non-selective susceptibility to ischemia of py-ramidal neurons in the CA3 area of the hippocampus is important from the point of view of elu-cidating the mechanisms of memory loss and the development of post-ischemic dementia. We used an ischemic model of Alzheimer's disease to study changes in amyloid protein precursor gene expression, its cleavage enzymes and tau protein in the CA3 area of the hippocampus af-ter a 10-minute brain ischemia with 12, 18, and 24-month survival. Quantitative reverse tran-scriptase PCR assay showed that the expression of all the genes that contribute to amyloid pro-duction was dysregulated within 2 years in the CA3 area of the hippocampus after ischemia. The expression of the amyloid protein precursor gene was above the control values at all times of the study. The expression of the α-secretase gene also exceeded the control values throughout the study. In contrast, the expression of the β-secretase gene reaching its maximum increase 12 months after ischemia, was below control values after 18 months and again above control values after 24 months of survival. Presenilin 1 and 2 gene expression was significantly elevated throughout the follow-up period, with peak expression of both genes occurring 12 months after ischemia. This suggests that the genes studied are involved in the non-amyloidogenic processing of amyloid protein precursor. Also, tau protein gene expression was significantly elevated throughout the observation period, and peak gene expression was present 12 months after is-chemia. Data indicate that an episode of brain ischemia with long-term survival causes damage and death of pyramidal neurons in the CA3 area of the hippocampus in a manner dependent on modified tau protein. Thus defining a new and important mechanism of pyramidal neuronal death in the CA3 area after ischemia. In addition tau protein gene modification after brain is-chemia is useful in identifying ischemic mechanisms occurring in Alzheimer's disease.

Preprint REVIEW | doi:10.20944/preprints201806.0302.v1

UNDERSTANDING THE ROLE OF DYSFUNCTIONAL AND HEALTHY MITOCHONDRIA IN STROKE PATHOLOGY AND ITS TREATMENT

Hung Nguyen, Sydney Zarriello, Mira Rajani, Julian Tuazon, Eleonora Napoli, Cesar Borlongan

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cerebral ischemia, blood brain barrier, endothelial cells, impaired mitochondria, neurovascular unit, regenerative medicine, stem cell therapy, transfer of healthy mitochondria, vasculature

Online: 19 June 2018 (14:27:25 CEST)

Show abstract| Download PDF| Share

Preprint ARTICLE | doi:10.20944/preprints202112.0041.v1

Genetics of Vascular Inflammation in Lower Limb Ischemia: The TNF-α Hypothesis in Peripheral Arterial Disease

Diego Caicedo, Clara V. Álvarez, Sihara Pérez, Jesús Devesa

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Vascular inflammation; Peripheral arterial disease (PAD); Chronic limb-threatening ischemia (CLTI); GHAS trial; TNF-α; hs-CRP; Neutrophil-to-lymphocyte ratio (NLR); NOX4; eNOS; VEGF-A.

Online: 3 December 2021 (09:44:42 CET)

Show abstract| Download PDF| Share

Preprint REVIEW | doi:10.20944/preprints202111.0253.v1

Personalized Cell Therapy for Patients With Peripheral Arterial Diseases in the Context of Genetic Alterations: Artificial Intelligence-based Responder and Non-responder Prediction

Amankeldi Salybekov, Markus Wolfien, Shuzo Kobayashi, Takayuki Asahara

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Cell therapy; chronic limb-threating ischemia; peripheral artery disease; diabetes; atherosclerosis obliterans; thromboangiitis obliterans; personalized medicine; artificial intelligence; machine learning; genome-wide association studies; transcriptome-wide association studies; clonal hematopoiesis of indeterminate potential.

Online: 15 November 2021 (11:18:43 CET)

Show abstract| Download PDF| Share

Search Results

93 articles found