PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Effects of Sildenafil on the Recovery of Cognitive Function and the Protection of Neuronal Cell Death Following Transient Global Cerebral Ischemia in Gerbils
Version 1
: Received: 21 May 2024 / Approved: 21 May 2024 / Online: 21 May 2024 (12:49:16 CEST)
How to cite:
Yu, Y. H.; Kim, G. W.; Lee, Y. R.; Park, D.-K.; Song, B.; Kim, D.-S. Effects of Sildenafil on the Recovery of Cognitive Function and the Protection of Neuronal Cell Death Following Transient Global Cerebral Ischemia in Gerbils. Preprints2024, 2024051364. https://doi.org/10.20944/preprints202405.1364.v1
Yu, Y. H.; Kim, G. W.; Lee, Y. R.; Park, D.-K.; Song, B.; Kim, D.-S. Effects of Sildenafil on the Recovery of Cognitive Function and the Protection of Neuronal Cell Death Following Transient Global Cerebral Ischemia in Gerbils. Preprints 2024, 2024051364. https://doi.org/10.20944/preprints202405.1364.v1
Yu, Y. H.; Kim, G. W.; Lee, Y. R.; Park, D.-K.; Song, B.; Kim, D.-S. Effects of Sildenafil on the Recovery of Cognitive Function and the Protection of Neuronal Cell Death Following Transient Global Cerebral Ischemia in Gerbils. Preprints2024, 2024051364. https://doi.org/10.20944/preprints202405.1364.v1
APA Style
Yu, Y. H., Kim, G. W., Lee, Y. R., Park, D. K., Song, B., & Kim, D. S. (2024). Effects of Sildenafil on the Recovery of Cognitive Function and the Protection of Neuronal Cell Death Following Transient Global Cerebral Ischemia in Gerbils. Preprints. https://doi.org/10.20944/preprints202405.1364.v1
Chicago/Turabian Style
Yu, Y. H., Beomjong Song and Duk-Soo Kim. 2024 "Effects of Sildenafil on the Recovery of Cognitive Function and the Protection of Neuronal Cell Death Following Transient Global Cerebral Ischemia in Gerbils" Preprints. https://doi.org/10.20944/preprints202405.1364.v1
Abstract
Cerebral ischemic stroke poses a significant global cause of death, with ischemia-reperfusion injury contributing to neuronal cell death and tissue damage. However, significant therapeutic approaches with meaningful treatment effects for patients with ischemic stroke in actual clinical practice are lacking. This study investigated the potential neuroprotective effects of sildenafil, a phosphodiesterase-5 inhibitor, in a global cerebral ischemia model. We explored the impact of sildenafil on GFAP and AQP-4 expression, which are markers associated with astrocyte activation and water homeostasis, respectively. The immunofluorescence analysis revealed that the number of cells co-expressing the markers, which increased in the ischemia-induced group, was significantly reduced in sildenafil-treated groups, suggesting a potential mitigating effect on ischemia-induced astrocyte activation. Additionally, we conducted diverse behavioral tests, including the open-field test, novel object recognition, Barnes maze, Y-maze, and passive avoidance tests, to assess the effect of sildenafil on the cognitive function impaired by ischemia. Cognitive improvements were observed following sildenafil administration (20mg/kg) compared to the untreated group. Taken together, the results presented here suggest sildenafil's potential as a neuroprotective agent for alleviating delayed neuronal cell death and cognitive function impaired by ischemia.
Keywords
global ischemia; reperfusion; sildenafil; behavioral test; neuroprotection
Subject
Medicine and Pharmacology, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
