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Preprint ARTICLE | doi:10.20944/preprints202301.0392.v1

Distinctive Signs of Disease as Deterrents for the Endothelial Function: A Systematic Review

Francesco Nappi, Sanjeet Singh Avtaar Singh

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Endothelial dysfunction; inflammation; pulmonary hypertension; diabetes; car-diovascular disease; endothelium-derived relaxing factor

Online: 23 January 2023 (02:23:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202205.0089.v1

HSPA1B is the Dominant Partner in the HSPA1A/HSPA1B Mediated Proteotoxic Stress Response in Placenta-Derived Stem Cells

Bothina Mohammed Alharbi, Aisha Bugshan, Azhaar Almozel, Reem Alenzi, Abderrezak Bouchama, Tanvir Khatlani, Sameer Mohammad, Shuja Shafi Malik

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: placenta-derived stem cells; placenta; stem cells; proteostasis; heat-shock; chaperones; HSPA1B; HSPA1A

Online: 7 May 2022 (03:37:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0523.v1

Recycling of Marine Carbonate Induced Calcium Isotope Heterogeneity of Arc Magmas in Subduction Zones

Xue-Gang Chen, Tao Wu, Qin Gao, Yu-Ming Lai

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: Carbonate recycling; Ca isotopes; Subduction zone; Sediment melts; Arc magmas; Slab-derived fluids

Online: 22 July 2021 (16:57:46 CEST)

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Calcium (Ca) is an essential element constituting sedimentary carbonate in subducting sediments. Ca isotopic characteristics of subduction-related rocks could provide insight into the behavior and budget of carbonate and carbon cycles in subduction zones, due to the distinctive δ^44/40Ca ranges of sedimentary carbonate with respect to the mantle. Here, we studied the Ca isotopic compositions of arc magmas from the Northern Luzon arc (NLA), which are evolved from a depleted mantle metasomatized by slab-derived fluids and sediment melts. The δ^44/40Ca values range from 0.76 ± 0.04‰ to 1.01 ± 0.03‰ and cover the typical ranges for bulk silica earth (BSE, ~ 0.94‰) and fresh mid-ocean ridge basalt (MORB, ~ 0.83‰). The Ca isotopes of NLA volcanics are not dominantly determined by the effects of mantle partial melting or fractional crystallization, nor significantly modified by secondary alteration. Instead, the δ^44/40Ca values of NLA volcanics are controlled by the subduction-related metasomatism. The metasomatism by slab-derived fluids (mainly expelled from altered oceanic crust, AOC) dramatically elevated the contents of fluid-mobile elements (e.g., Ba and Pb) with respect to fluid-immobile elements (e.g., Ce). This process, however, rarely modified the Ca isotopes, possibly ascribed to the δ^44/40Ca similarity between AOC and the depleted mantle. The δ^44/40Ca values significantly correlated with subduction indicators (e.g., Sr-Nd isotopes, Ba/Nb, Ce/Pb, and Nb/La), demonstrating the Ca isotopes of NLA volcanics are mainly controlled by the metasomatism of sediment melts subducting from the South China Sea (SCS). Based on the thermal structures and chemical compositions of sediments subducting into global trenches, we propose that carbonate Ca isotopic signals can only be observed in the arcs with high sedimentary Ca fluxes and temperature-pressure conditions well beyond the solidus of H₂O-saturated sediment melting, e.g., NLA, Nicaragua, Guatemala, Colombia, Peru, South Chile, North Vanuatu, New Zealand, and Kermadec. The absence of such signals in other arcs suggests either limited sedimentary fluxes or much of the subducting sedimentary carbonate has been survived during plate subduction to enter the deep mantle.

Preprint ARTICLE | doi:10.20944/preprints202011.0747.v1

Theory of g-T_g-Derived and g-T_g-Coderived Operators: Definitions, Essential Properties, Iterations, and Ranks

Mohammad Irshad KHODABOCUS, Noor-Ul-Hacq SOOKIA

Subject: Computer Science And Mathematics, Geometry And Topology Keywords: Generalized topological space, generalized sets, generalized derived operator, generalized coderived operator, iterations, ranks

Online: 30 November 2020 (20:21:55 CET)

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In a generalized topological space T_g = (Ω, T_g) (T_g-space), the g-topology T_g : P (Ω) −→ P (Ω) can be characterized in the generalized sense by specifying the generalized open, generalized closed sets (g-T_g-open, g-T_g-closed sets), generalized interior, generalized closure operators g-Intg, g-Cl_g : P (Ω) −→ P (Ω) (g-T_g-interior, g-T_g-closure operators), or generalized derived, generalized coderived operators g-Der_g, g-Cod_g : P (Ω) −→ P (Ω) (g-T_g-derived, g-T_g-coderived operators), respectively. For very many T_g-spaces, the δ^th-iterates g-Der_g^(δ), g-Cod_g^(δ) : P (Ω) −→ P (Ω) of g-Der_g, g-Cod_g : P (Ω) −→ P (Ω), respectively, defined by transfinite recursion on the class of successor ordinals are also themselves g-T_g-derived, g-T_g-coderived operators for new g-topologies in the generalized sense on Ω. Thus, the use of novel definitions of g-T_g-derived, g-T_g-coderived operators g-Der_g, g-Cod_g : P (Ω) −→ P (Ω), respectively, based on a very clever construction, together with their δ^th-iterates g-T_g-operators g-Der_g^(δ), g-Cod_g^(δ) : P (Ω) −→ P (Ω), defined by transfinite recursion on the class of successor ordinals, will give rise to novel generalized g-topologies on Ω. The present authors have been actively engaged in the study of g-T_g-operators in T_g-spaces. The study of the essential properties and the commutativity of novel definitions of g-T_g-interior and g-T_g-closure operators g-Int_g, g-Cl_g : P (Ω) −→ P (Ω), respectively, in T_g has formed the first part, and the study of the essential properties and sets of consistent, independent axioms of novel definitions of g-T_g-exterior and g-T_g-frontier operators g-Ext_g, g-Fr_g : P (Ω) −→ P (Ω), respectively, has formed the second part. In this work, which forms the last part on the theory of g-T_g-operators in T_g-spaces, the present authors propose to present novel definitions and the study of the essential properties of g-T_g-derived and g-T_g-coderived operators g-Der_g, g-Cod_g : P (Ω) −→ P (Ω), respectively, and their δ^th-iterates, and the notions of g-T_g-open and g-T_g-closed sets of ranks δ in T_g-spaces.

Working Paper ARTICLE

Temperate Propolis Has Anti-inflammatory Effects and Is a Potent Inhibitor of Nitric Oxide Formation in Macrophages

Samyah Alanazi, Naif Alenzi, James Fearnley, W. Harnett, David Watson

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: propolis; pro- and anti-inflammatory cytokines; LPS stimulation; bone marrow derived macrophages; metabolomics

Online: 14 September 2020 (00:36:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints201806.0432.v1

Synthetic and Marine-Derived Porous Bone Graft Substitutes

Ana Neto, José Maria Ferreira

Subject: Chemistry And Materials Science, Biomaterials Keywords: Bone tissue engineering; biomaterials; bone scaffolds; additive manufacturing techniques/robocasting; marine-derived biomaterials

Online: 26 June 2018 (16:05:49 CEST)

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Preprint REVIEW | doi:10.20944/preprints202111.0090.v2

Why and How to Use the Body’s Own Stem Cells for Regeneration in Musculoskeletal Disorders: A Primer

John Furia, Mark Lundeen, Jason Hurd, David Pearce, Christopher Alt, Eckhard Alt, Christoph Schmitz, Nicola Maffulli

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: adipose-derived regenerative cells; ADRCs; adipose-derived stem cells; ADSCs; bone regeneration, cartilage regeneration; efficacy; point of care treatment; stem cell; tendon healing without scar formation; tendon regeneration; vaPS cells

Online: 12 November 2021 (11:44:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0598.v1

Kyogo Itoh, Shigeki Shichijo, Shigetaka Suekane

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: anti-tumor immunity; antigenic mimicry; CTL epitope peptides; human tumor-associated antigens; intestinal microbiota; linear sequence analysis; TCR-mediated recognition analysis

Online: 10 October 2023 (08:31:28 CEST)

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Preprint REVIEW | doi:10.20944/preprints201808.0489.v1

Chemotherapy-exacerbated Breast Cancer Metastasis: A Paradox Explainable by Stress Response

Justin D. Middleton, Daniel G. Stover, Tsonwin Hai

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: chemotherapy; breast cancer metastasis; stress response; ATF3; seed and soil theory; cancer-host interaction; tumor microenvironment; immune modulation; tumor immune environment

Online: 29 August 2018 (09:05:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1058.v1

Mesothelioma Associated Fibroblasts Modulate the Response of Mesothelioma Patient Derived Organoids to Chemotherapy through Interleukin-6.

Mario Cioce, Veronica Gatti, Fabiana Napolitano, Noemi Maria Giorgiano, Andrea Marra, Giuseppe Portella, Alfonso Fiorelli, Francesca Pentimalli, Vito Michele Fazio

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Mesothelioma patient-derived organoids, PDO, IL-6, mesothelioma-associated fibroblasts; cocultures, cisplatin; pemetrexed; chemoresistance

Online: 18 March 2024 (15:17:01 CET)

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Background. Malignant pleural mesothelioma (MPM) is still a rather incurable disease. Many classes of compounds with various mechanisms of action have been tested in vivo and ex-vivo, with rather disappointing results. This is partly due to preclinical studies mostly performed in models that fail to recapitulate MPM complexity and do not retain the intra-tumor heterogeneity, a propelling force for tumor progression. Additionally, we and others have shown that, under stress-induced chemotherapy, profound rearrangements take place in MPM cultures to fuel the emergence of chemoresistant cell subpopulations. Thus, finding clinically actionable modulators of MPM resistance is still a very urgent need. Methods. Setup and propagation of patient-derived organoid cultures from MPM pleural effusions; flow cytometry-based validation of organoid cultures, isola-tion and propagation of mesothelioma-associated fibroblasts; co-cultures, Lu-minex-based Cytokine array, ELISA assay to quantitate IL-6 in the conditioned media, qRT-PCR to assess mRNA expression of cancer stem cell markers and fibroblast markers. Results. Organoid cultures were obtained from eight MPM patients by using specific growth media and conditions to propagate pleural effusion-derived cells. We proved that primary MPM specimens and serially passaged (mesothelio-ma-Patient-Derived-Organoids) mPDOs were cytologically similar, with the obtained mPDOs expressing mesothelial markers similarly to the originating specimen. As expected from a clinically relevant model, the size and number of mPDOs were altered, in a patient-specific way, when pharmacologically relevant doses of chemotherapy drugs were added. We derived Mesothelio-ma-Associated-Fibroblasts (MAFs) and the addition of MAFs, to various ratios, altered the response of the mPDOs to pemetrexed + cisplatin, toward a reduced sensitivity to the latter anticancer agents. Additionally, organoid formation and size were altered by MAF-conditioned media, and this correlated with increased expression of cancer stem cell markers. We showed increased levels of IL-6 in the media of the treated cocultures and showed that this latter was derived from the MAFs. Conclusions. Increased secretion of IL-6 by mesothelioma-associated fibroblasts (MAFs) contributes to the resistance of the MPM to therapy.

Preprint REVIEW | doi:10.20944/preprints202401.1321.v1

Examining Natural Products with the Potential to Chagas Disease: An In-depth Analysis

Eze Elijah Ajaegbu, Chizurum Philip Ikegbuna

Subject: Medicine And Pharmacology, Pharmacy Keywords: Chagas disease; Trypanosoma cruzi; Natural products; Therapeutic approaches; Anti-parasitic compounds; Plant-derived compounds

Online: 17 January 2024 (12:04:26 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1527.v1

The Functional Roles of MDSCs in Severe COVID-19 Pathogenesis

Kuan Rong Chan, Jia Soon Len, Clara WT Koh

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; myeloid-derived suppressor cells; infection; innate immunity; T cells

Online: 23 November 2023 (11:18:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.1215.v1

Dietary Supplementation of a Mixture of Nucleotides, β-Glucan and Vitamins C and E Improved the Growth and Health Performance of Olive Flounder,Paralichthys olivaceus

Nalin Medagoda, Rutchanee Chotikachinda, Mirasha Hasanthi, Kyeong-Jun Lee

Subject: Biology And Life Sciences, Aquatic Science Keywords: Nucleotides; Saccharomyces cerevisiae derived β-glucan; Vitamins C and E; Innate-immunity; Olive flounder

Online: 29 April 2023 (05:30:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202302.0290.v1

Neuroprotective Effect of Curcumin on Factors Involved in Neurogenesis and Angiogenesis in Aged Brain

Masoumeh Habibian, Seyyed Jafar Moosavi

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: aging; Apoptosis; Brain-derived neurotrophic factor; transforming growth factor β1; vascular endothelial growth factor

Online: 17 February 2023 (02:25:34 CET)

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Preprint ARTICLE | doi:10.20944/preprints202108.0536.v1

Alphavirus-driven Interferon Gamma (IFNg) Expression Inhibits Tumour Growth in Orthotopic 4T1 Breast Cancer Model

Olga Trofimova, Ksenija Korotkaja, Dace Skrastina, Juris Jansons, Karina Spunde, Maria Isaguliants, Anna Zajakina

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: interferon gamma; cancer immunotherapy; viral vectors; alphavirus; bone marrow-derived macrophages; spheroids; CD38; Pam3CSK4

Online: 30 August 2021 (10:18:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202108.0004.v1

Extracellular Vesicles in Skin Wound Healing

Deimantė Narauskaitė, Gabrielė Vydmantaitė, Justina Rusteikaitė, Revathi Sampath, Akvilė Rudaitytė, Gabija Stašytė, María Isabel Aparicio Calvente, Aistė Jekabsone

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: extracellular vesicles; EVs; exosomes; mesenchymal stem cell EVs; plant-derived EVs; wound healing; scaffolds

Online: 2 August 2021 (08:57:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202101.0545.v3

Evaluation of High-Resolution Satellite-Derived Solar Radiation Data for PV Performance Simulation in East Africa

Diane Palmer, Richard Blanchard

Subject: Engineering, Energy And Fuel Technology Keywords: Solar radiation; Satellite-derived irradiance; Global Horizontal Irradiance; Clear sky model; ground stations; validation

Online: 18 March 2021 (14:30:42 CET)

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Working Paper ARTICLE

Safety and Efficacy of Treating Symptomatic, Partial-Thickness Rotator Cuff Tear with Fresh, Uncultured, Unmodified, Autologous Adipose Derived Regenerative Cells (UA-ADRCs) Isolated at the Point of Care: A Prospective, Randomized, Controlled First-in-Human Pilot Study

Jason L. Hurd, Tiffany R. Facile, Jennifer Weiss, Matthew Hayes, Meredith Hayes, John P. Furia, Nicola Maffulli, Glenn E. Winnier, Christopher Alt, Christoph Schmitz, Eckhard U. Alt, Mark A. Lundeen

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: adipose-derived regenerative cells; ADRCs; partial rotator cuff tear; stem cells; stromal vascular fraction

Online: 14 February 2020 (05:05:43 CET)

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Background: This study tested the hypothesis that treatment of symptomatic, partial-thickness rotator cuff tear (sPTRCT) with fresh, uncultured, unmodified, autologous adipose derived regenerative cells (UA-ADRCs) isolated from lipoaspirate at the point of care is safe and more effective than corticosteroid injection. Methods: Subjects aged between 30 and 75 years with sPTRCT who did not respond to physical therapy treatments for at least six weeks were randomly assigned to receive a single injection of an average 11.4×10⁶ UA-ADRCs (in 5 mL liquid; mean cell viability: 88%) (n=11; modified intention-to-treat (mITT) population) or a single injection of 80 mg of methylprednisolone (40 mg/ml; 2 mL) plus 3 mL of 0.25% bupivacaine (n=5; mITT population), respectively. Safety and efficacy were assessed using the American Shoulder and Elbow Surgeons Standardized Shoulder Assessment Form (ASES), RAND Short Form-36 Health Survey and pain visual analogue scale (VAS) at baseline (BL) as well as three weeks (W3), W6, W9, W12, W24, W32, W40 and W52 post treatment. Fat-saturated T2 weighted magnetic resonance imaging of the shoulder was performed at BL as well as at W24 and W52 post treatment. Results: No severe adverse events related to the injection of UA-ADRCs were observed in the 12 months post treatment. The risks connected with treatment of sPTRCT with UA-ADRCs were not greater than those connected with treatment of sPTRCT with corticosteroid injection. However, one subject in the corticosteroid group developed a full rotator cuff tear during the course of this pilot study. Despite the small number of subjects in this pilot study, those in the UA-ADRCs group showed statistically significantly higher mean ASES total scores at W24 and W52 post treatment than those in the corticosteroid group (p < 0.05). Discussion: This pilot study suggests that the use of UA-ADRCs in subjects with sPTRCT is safe and leads to improved shoulder function without adverse effects. To verify the results of this initial safety and feasibility pilot study in a larger patient population, a randomized controlled trial on 246 patients suffering from sPTRCT is currently ongoing.

Preprint ARTICLE | doi:10.20944/preprints202405.0739.v1

Alterations in Graph Network Connectivity Predict Neurocognitive Changes in Patients Undergoing Brain Tumor Surgery

David G Ellis, David Warren, Matthew Garlinghouse, Michele R Aizenberg

Subject: Neuroscience And Neurology, Medicine And Pharmacology Keywords: Brain connectivity; brain tumor; graph theory; connectome; neuropsychological evaluation

Online: 13 May 2024 (07:47:23 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0692.v1

Pancreatic Neuroendocrine Tumors: What Is the Best Surgical Option?

Renato Patrone, Federico Maria Mongardini, Alessandra Conzo, Chiara Cacciatore, Giovanni Cozzolino, Antonio Catauro, Eduardo Lanza, Francesco Izzo, Andrea Belli, Raffaele Palaia, Luigi Flagiello, Ferdinando De Vita, Ludovico Docimo, Giovanni Conzo

Subject: Medicine And Pharmacology, Surgery Keywords: pNET; Neuroendocrine tumors; Pancreas tumor; pancreatic cancer; enucleation; Pancreatectomy

Online: 10 April 2024 (13:05:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1500.v1

Defucosylated Anti-CD44 Monoclonal Antibodies Exert Antitumor Activities against Esophageal Tumor Xenograft

Kenichiro Ishikawa, Hiroyuki Suzuki, Tomokazu Ohishi, Akira Ohkoshi, Mika K Kaneko, Yukio Katori, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: CD44; monoclonal antibody; ADCC; CDC; esophageal tumor; antitumor activity

Online: 26 March 2024 (08:14:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1608.v1

Molecular Mechanisms of Erastin-Induced Ferroptosis in Human Ovarian Tumor Cells

Birandra K Sinha, Murphy Carri, Shalyn M Brown, Brian Siver, Erik J Tokar, Carl D Bortner

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Erastin; Ferroptosis; Ovarian Tumor calls; RSL3; Reactive Oxygen Species

Online: 28 February 2024 (06:56:27 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.0092.v1

Mechanism of Action of Intestinal Microorganisms and Progress in Head and Neck Tumors

Xialu Su, Faqiang Ma, Feng Jin

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: intestinal flora; head and neck tumors; tumor immunity; therapy

Online: 3 January 2024 (07:36:32 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.1417.v2

Cellular Response to an Act of Surviving Drives Tumor Evolution and Tumor Heterogeneity

Yuntian Zhang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: tumor heterogeneity; cancer progression; oncogene addiction; cancer stem cell

Online: 13 November 2023 (10:39:49 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.1503.v1

Lambert Eaton Myasthenic Syndrome: A Bridge Diagnostic For Occult Tumor

Marcelo De Moura Campos, Eduardo Pagliarini, Amanda Locks, Marcus Gonçalves

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Lambert-Eaton; Paraneoplastic Syndrome; Tumor; SCLC; Antibodies; COVID-19

Online: 24 October 2023 (08:39:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1204.v1

Alterations in Intratumoral Immune Response Before and Early-on-Treatment of Nivolumab in Unresectable Advanced or Recurrent Gastric Cancer

Yasuyoshi Sato, Hiroharu Yamashita, Yukari Kobayashi, Koji Nagaoka, Tetsuro Hisayoshi, Takuya Kawahara, Akihiro Kuroda, Noriyuki Saito, Ryohei Iwata, Yasuhiro Okumura, Koichi Yagi, Susumu Aiko, Sachiyo Nomura, Kazuhiro Kakimi, Yasuyuki Seto

Subject: Medicine And Pharmacology, Other Keywords: gastric cancer; Nivolumab; immune response; tumor microenvironment; RNA-Seq

Online: 19 October 2023 (03:29:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0227.v1

Environmental Enrichment in Cancer: A Possible Tool to Combat Tumor Development: A Systematic Review

Matheus Santos Fernandes, Tiago Ramos Lacerda, Débora Eduarda da Silva Fidelis, Gabriela Carvalho Jurema Santos, Tayrine Ordonio Filgueira, Raphael Fabrício De Souza, Claúdia Jacques Lagranha, Fábio Santos Lira, Angela Castoldi, Fabricio Oliveira Souto

Subject: Biology And Life Sciences, Life Sciences Keywords: Enriched Environment; Cancer; Tumor growth; angiogenesis; pro-oncogenic factor

Online: 4 October 2023 (09:35:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.1432.v1

Adding Value to Liquid Biopsy for Brain Tumors: The Role of Imaging

Nastaran Khalili, Hossein Shooli, Nastaran Hosseini, Anahita Fathi Kazerooni, Ariana Familiar, Sina Bagheri, Hannah Anderson, Stephen J. Bagley, Ali Nabavizadeh

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Imaging; Liquid biopsy; glioma; glioblastoma; focused ultrasound; tumor biomarkers

Online: 21 September 2023 (07:24:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.2027.v1

Analysis of Gene Expression Networks in Primary Central Nervous System Tumors for Drug Repurposing Prospects

Brian Harvey Avanceña Villanueva, Po-Wei Tsai, Lemmuel L. Tayo

Subject: Biology And Life Sciences, Other Keywords: central nervous system tumor; co-expression network; drug repurposing

Online: 30 August 2023 (09:04:04 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1188.v1

β2-Adrenergic Receptor Mediated Inhibition of T Cell Function And Its Implications For CAR-T Cell Therapy

Muhammad Asad Farooq, Iqra Ajmal, Xinhui Hui, Yiran Chen, Renzheng Yang, Wenzheng Jiang

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: CAR-T therapy, Immunosuppression, Tumor microenvironment, adrenergic stress,; immunotherapy

Online: 18 July 2023 (10:29:29 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202306.1673.v1

Unintentional Two-Stage Surgery of Hypervascular Choroid Plexus Papilloma considering Venous Drainage: A Case Report

Daishiro Abe, Kohei Kanaya, Takafumi Kiuchi, Sumio Kobayashi, Kenji Sano, Tetsuyoshi Horiuchi

Subject: Medicine And Pharmacology, Surgery Keywords: choroid plexus papilloma; hypervascular tumor; surgical strategy; venous drainage

Online: 23 June 2023 (12:00:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202108.0223.v1

Characteristics of Nephroblastoma / Nephroblastomatosis in Children With a Clinically Reported Underlying Malformation or Cancer Predisposition Syndrome

Nils Welter, Angelo Wagner, Rhoikos Furtwängler, Patrick Melchior, Leo Kager, Christian Vokuhl, Jens-Peter Schenk, Clemens Magnus Meier, Stefan Siemer, Manfred Gessler, Norbert Graf

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: nephroblastoma; clinical malformations; cancer predisposition syndromes; tumor surveillance; outcome

Online: 10 August 2021 (09:55:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202107.0551.v1

Receptor Tyrosine Kinases as Candidate Prognostic Biomarkers in Meningioma

Rafael Roesler, Barbara Kunzler Souza, Gustavo R. Isolan

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: epidermal growth factor receptor; ErbB; biomarker; meningioma; intracranial tumor

Online: 23 July 2021 (22:06:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0527.v1

Fate of Surgical Patients with Small Nonfunctioning Pancreatic Neuroendocrine Tumors: An International Study Using Multi-Institutional Registries

In Woong Han, Jangho Park, Eun Young Park, So Jeong Yoon, Gang Jin, Dae Wook Hwang, Kuirong Jiang, Wooil Kwon, Xuefeng Xu, Jin Seok Heo, Delinag Fu, Woo-Jung Lee, Xueli Bai, Yoo-Seok Yoon, Yinmo Yang, Keun Soo Ahn, Chunhui Yuan, Hyeon Kook Lee, Bei Sun, Eun Kyu Park, Seung Eun Lee, Sunghwa Kang, Wen hui Lou, Sang-Jae Park

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Nonfunctioning neuroendocrine tumor of pancreas; Prognosis; Resection; Risk factors

Online: 23 July 2021 (08:07:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202008.0641.v2

Brain Tumor Detection based on Ensemble Learning

Donghyun Kim

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: brain tumor; machine learning; ensemble methods; convolutional neural networks

Online: 8 June 2021 (13:53:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0573.v1

A Surgical Pen-Type Probe Design for Real-Time Optical Diagnosis of Tumor Status using 5-Aminolevulinic acid

Ki-Cheol Yoon, Kwang Gi Kim, Seung Hoon Lee

Subject: Engineering, Automotive Engineering Keywords: fluorescence microscopy; fluorescence emission, malignant tumor, diagnosis, animal experiment

Online: 21 April 2021 (11:47:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0557.v1

Design of a Surgical Pen-Type Probe for Real-Time Diagnosis of Vascular Flow Using Indocyanine Green (ICG) Fluorescence Dye

Ki-Cheol Yoon, Kwang Gi Kim, Seung Hoon Lee

Subject: Engineering, Automotive Engineering Keywords: fluorescence microscopy; fluorescence emission; malignant tumor; diagnosis; animal experiment

Online: 21 April 2021 (08:30:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202102.0315.v1

Characterisation of Immune Cell Subsets of Tumour Infiltrating Lymphocytes in Brain Metastases

Priyakshi Kalita-de Croft, Haarika Chittoory, Tam Nguyen, Jodi Saunus, Woo Gyeong Kim, Amy McCart Reed, Malcolm Lim, Xavier De Luca, Kaltin Ferguson, Colleen Niland, Roberta Mazzieri, Riccardo Dolcetti, Peter Simpson, Sunil R Lakhani

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Brain Metastasis; Immune Checkpoint; Tumor Microenvironment, T-cells, TILs

Online: 15 February 2021 (15:06:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints202102.0074.v1

Impact of Physical Activity on Recurrence Dynamics in Early Breast Cancer Patients

Elia Biganzoli, Christine Desmedt, Romano Demicheli

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: early breast cancer; prognosis; physical activity; tumor dormancy; recurrence

Online: 2 February 2021 (08:24:31 CET)

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Several studies have suggested that pre and/or postdiagnosis physical activity can reduce the risk of recurrence in breast cancer patients, however its effect according to follow-up time has not yet been investigated. We analyzed recurrence and mortality dynamics in randomized clinical trials (RCTs) from Australia and Canada. The combined Australian RCTs evaluated, at median follow-up of 8.3 years, an 8-month pragmatic exercise intervention in 337 women with newly diagnosed breast cancer, while the Canadian RCT evaluated, at median follow-up of 7.4 years, supervised aerobic or resistance exercise during chemotherapy in 242 patients. For each RCT, the control arm consisted of patients undergoing usual care. We estimated the event dynamics by the discrete hazard function, through flexible regression of yearly conditional event probabilities with generalized additive models. In the considered RCTs, the recurrence and mortality risk of patients enrolled in the physical activity arm was stably reduced at medium/long term after five year of follow-up. In the Australian RCTs where patients were recruited by urban versus rural area, the latter group did not display benefit from physical activity. Estimated Odds Ratios (95% Confidence Intervals) for Disease Free Survival (DFS) in urban women were 0.63 (0.22-1.85); 0.27 (0.079-0.90); 0.11 (0.013-0.96) at the 3rd, 5th and 7th year of follow-up, respectively. For rural women, DFS patterns were overlapping with ORs approximating 1 at the different years of follow-up. Although not reaching statistical evidence, the estimates in the Canadian trial were in line with the results from the Australian urban women with ORs (95% CI) forDFS of 0.70 (0.33-1.50); 0.47 (0.19-1.18); 0.32 (0.077-1.29) at 3rd, 5th, 7th follow-up year, respectively. While we acknowledge that the analyzed RCTs were not designed for investigating disease recurrence over time, these results support the evidence that physical activity reduces the risk of developing medium/long-term metastases. Additional translational research is needed to clarify the mechanisms underlying these observations.

Preprint REVIEW | doi:10.20944/preprints202101.0307.v1

Female Germ Cell Development, Functioning and Associated Adversities Under Unfavorable Circumstances

Dinesh Bharti, Manisha Tikka, Sang-Yun Lee, Eun-Yeong Bok, Hyeon-Jeong Lee, Gyu Jin Rho

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: gametogenesis; oocytes; pathways; signaling; stem cells; differentiation; tumor; cancer

Online: 18 January 2021 (09:14:54 CET)

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Working Paper REVIEW

Integrating Conflicting Cancer Theories by Recognizing the Roles of Epigenetic and Genetic Alterations in the Immediate-Cancer-Causing Genes that Establish Cellular Immortality and Autonomy

Shengming Zhu, Jiangang Wang, Haiyan Zhou, Yan Zhao, Yan He, Lucas Zellmer, Zhiguo Luo, Sixi Wei, Fei Deng, Hai Huang, Dezhong Joshua Liao

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Mutation; Epigenetic; Genetic; Neoplastic transformation; Stem cell; Tumor classification

Online: 30 November 2020 (08:26:34 CET)

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Preprint REVIEW | doi:10.20944/preprints202004.0479.v1

Need for Specialized Therapeutic Stem Cells Banks Equipped with Tumor Regression Enzymes and Anti-Tumor Genes

Mujib Ullah

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Stem cells banks; Cancer; Cytosine deaminase; Tumor; Gene therapy

Online: 27 April 2020 (04:49:54 CEST)

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Working Paper ARTICLE

Promoter Hypermethylation of Tumor Suppressor Genes, p16^INK4a, RASSF1A, TIMP3 and PCQAP/MED15, in Salivary DNA as a Quadruple Biomarker Panel for Early Detection of Oral and Oropharyngeal Cancers

Chamikara Liyanage, Asanga Wathupola, Sanjayan Muraleetharan, Kanthi Perera, Chamindie Punyadeera, Preethi Udagama

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Oral cancer; Oropharyngeal cancer; Tumor-suppressor genes; Promoter hypermethylation

Online: 19 March 2019 (12:56:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints201901.0283.v1

Evaluation of Anticancer Efficacy of Cyclosaplin Using a Silk Based 3D Tumor Model

Abheepsa Mishra, Sourav K. Mukhopadhyay, Satyahari Dey

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: breast cancer; cyclosaplin; 3D tumor model; peptide, sandalwood; silk

Online: 29 January 2019 (03:26:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0154.v1

Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse

Chinmayee Sethy, Kunal Goutam, Deepika Nayak, Rajalaxmi Pradhan, Sefinew Molla, Subhajit Chatterjee, Niranjan Rout, Michael Wyatt, Satya Narayan, Chanakya Kundu

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Nectin-4; metastasis; angiogenesis; circulating tumor cells; cancer relapse

Online: 8 October 2018 (15:52:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201809.0545.v1

miR-3687 Overexpression Promotes Bladder Cancer Cell Growth Through Inhibiting the Negative Effect of FOXP1 on Cyclin E2 Transcription

Qipeng Xie, Caiyi Chen, Haiying Li, Huiying Yan, Jiheng Xu, Dapang Rao, Huxiang Zhang, Honglei Jin, Haishan Huang, Chuanshu Huang

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: miR-3687, FOXP1, cyclin E2, Bladder Cancer, Tumor formation

Online: 27 September 2018 (13:05:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201805.0073.v1

Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy

Ji-Eun Chang, Yang Liu, Woo Kyoung Lee, Il Yoon, Kwhanmien Kim

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: cancer treatment; chlorin; Photodynamic therapy (PDT); photosensitizer; tumor size

Online: 3 May 2018 (12:16:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201705.0100.v1

Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Antigenotoxicity, Cytotoxicity and Apoptotic Effects

Damián Martínez-Valdivieso, Rafael Font, Zahira Fernández-Bedmar, Tania Merinas-Amo, Pedro Gómez, Ángeles Alonso-Moraga, Mercedes Del Río-Celestino

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: cancer; carotenoid; chemoprevention; Cucurbita; cytotoxicity; DNA-protection; tumor cells

Online: 11 May 2017 (17:31:47 CEST)

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Preprint ESSAY | doi:10.20944/preprints201704.0120.v2

Synthesis and Anti-Tumor Activity of Oleanolic Acid Derivatives

Kuai ZhenYu, Lei Li, Shengwen Zhan, Chunlin Li, Yuwei Zhao, Hongrong Chen, Yanqiu Meng

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: oleanolic acid derivatives; synthesis; anti-tumor activity; molecular docking; computer-aided drug design

Online: 24 April 2017 (12:08:36 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.2227.v1

Immune Cytolytic Activity and Strategies for Therapeutic Treatment

Stephanie Agioti, Apostolos Zaravinos

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Cytolytic Activity (CYT); Tumor-Infiltrating Lymphocytes (TILs); Gene Expression Profiling; Spatial Analysis Techniques; Immune Checkpoint Pathways; Tumor Microenvironment (TME); Antigen Presentation; Metabolic Pathways

Online: 31 January 2024 (12:32:26 CET)

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Preprint REVIEW | doi:10.20944/preprints202404.1113.v1

Evolving Biomarkers in Kidney Transplantation

Maurizio Salvadori, alberto rosati, Giuseppina Rosso

Subject: Medicine And Pharmacology, Transplantation Keywords: Kidney rejection; biomarkers; subclinical rejection; donor derived cell free DNA; gene expression profile; urinary cytokines

Online: 17 April 2024 (11:18:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1275.v1

OSMAC Method to Assess Impact of Culture Parameters on Metabolomic Diversity and Biological Activity of Marine-Derived Actinobacteria

Alexandre Le Loarer, Laurent Dufossé, Jérome Bignon, Michel Frédérich, Allison Ledoux, Mireille Fouillaud, Anne Gauvin-Bialecki

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: OSMAC method; marine-derived actinobacteria; molecular network; natural product; specialized metabolites; cytotoxic activity; antiplasmodial activity

Online: 19 September 2023 (08:50:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1933.v1

The Ubiquitin-Proteasome Pathway and p62/SQSTM1 are Involved in The Rapid Degradation and Aggregation Mechanisms of CHK1 Mutant-Derived Toxic AGEs Formed by Glyceraldehyde

Kenji Takeda, Akiko Sakai-Sakasai, Kouji Kajinami, Masayoshi Takeuchi

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: advanced glycation end-products (AGEs); glyceraldehyde (GA); glyceraldehyde-derived AGEs; toxic-AGEs (TAGE); p62/SQSTM1

Online: 26 May 2023 (11:53:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0321.v2

The Composition of Adipose-Derived Regenerative Cells Isolated from Lipoaspirate using a Novel Point of Care System does not Depend on the Subject's Individual Age, Gender, Body Mass Index and Ethnicity

Christoph Schmitz, Christopher Alt, Alon R. Azares, David A. Peace, Tiffany R. Facile, John P. Furia, Nicola Maffulli, Claire Huang, Eckhard U. Alt

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: adipose-derived regenerative cells; flow cytometry; microfragmented fat; NucleoCounter; regenerative medicine; stem cells; UA-ADRCs

Online: 7 November 2022 (12:12:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints201708.0065.v1

4SM Method Tested in the Gulf of California Suggests Field Data are Not Needed to Derive Satellite Bathymetry

Fabio Favoretto, Yann Morel, Andrew Waddington, Jorge Lopez-Calderon, Marco Cadena-Roa, Anidia Blanco-Jarvio

Subject: Biology And Life Sciences, Biophysics Keywords: 4SM; satellite derived bathymetry; water depth; water column correction; remote sensing; Landsat; San Lorenzo Channel

Online: 18 August 2017 (12:14:16 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202405.0826.v1

An Unusual Presentation of a Newly Diagnosed Right Colonic Adenocarcinoma Complicated with Right Lower Quadrant Colonic Abscess in an Elderly Man: A Case Report and Literature Review

Divine Besong Arrey Agbor, Victoria Palmer, Annmarie Theresa Sajeev, Derek Ugwendum, Sabastain Forsah, Sefakor Akosua Atadja, Nancelle Ndema, Zafar Wahib, Foma Munoh Kenne, Jay Nfonoyim

Subject: Oncology And Oncogenics, Medicine And Pharmacology Keywords: Colon cancer; colon abscess, colon adenocarcinoma; colon neoplasm; colorectal tumor

Online: 13 May 2024 (09:56:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0864.v1

Design of a Dispersive 1064 nm Fiber Probe Raman Imaging Spectrometer and Its Application to Human Bladder Resectates

Juan David Munoz-Bolanos, Tanveer Ahmed Shaik, Arkadiusz Miernik, Juergen Popp, Christoph Krafft

Subject: Biology And Life Sciences, Life Sciences Keywords: bladder tumor; Raman imaging; 1064 nm; cluster analysis; Python toolbox

Online: 15 April 2024 (09:07:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0157.v1

Stimuli-Sensitive Polymeric Micelles Formulations Enhance the Cytostatic Effect against the Leukemia Cells via “Aikido” Principle Realization

Igor D. Zlotnikov, Alexander A. Ezhov, Natalia Vladimirovna Dobryakova, Elena V. Kudryashova

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: glutathione sensitivity, tumor targeting, polymeric micelles, doxorubicin, non-target toxicity

Online: 2 February 2024 (14:08:44 CET)

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Currently, the most promising work seems to be aimed not so much at the development of new drugs, but rather at creating ways of targeted delivery of existing drugs. In this paper, we show that polymeric micelles based on chitosan, heparin grafted with lipoic and oleic acids residues demonstrate the potential for doxorubicin delivery to tumors due to pH-, glutathione-sensitivity. Smart delivery systems use the Aikido principle, i.e. they target tumor cells according to their features. Natural polysaccharides (heparin and chitosan) provide the pH sensitivity of micelles: the release of doxorubicin (Dox) is enhanced in a slightly acidic environment (model tumor microenvironment). Fatty acid residues are necessary for the formation of micelles and solubilization of cytostatics in a hydrophobic core. Lipoic acid residues formed a labile S-S cross-linking between polymer chains (the first method), and also covalently attached doxorubicin molecules through glutathione-sensitive S-S bridges (the second option) – determine Redox sensitivity of micelles to tumors. The characterization of polymeric micelles and prodrugs (Dox-polymer) was carried out using FTIR spectroscopy. The release of doxorubicin from micelles occurs slowly (20% / 6h) in an environment with a pH of 7.4 and the absence of glutathione, while in a slightly acidic environment and in the presence of 10 mM glutathione, the rate increases up 6 times, with an increase in the effective concentration up to 5 times after 7 h. The permeability of free doxorubicin, micellar formulations (covalent S-S crosslinked and not) into Raji, K562, A875 cancer cells was studied using confocal laser scanning microscopy (CLSM), demonstrated an increase in the permeability of micellar formulations based on Dox. Thus, we have presented micellar formulations of doxorubicin for targeted delivery to tumor, what is important in the aspects of reducing non-target toxicity, increasing the selectivity of cytostatics against tumors and overcome multidrug resistance.

Preprint REVIEW | doi:10.20944/preprints202402.0143.v1

Research Progress of NK Cell-Based Nanomaterials in Tumor Immunotherapy and Imaging

Yachan Feng, Haojie Zhang, Jiangtao Shao, Chao Du, Xiaolei Zhou, Xueling Guo, Yingze Wang

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: NK cell immunotherapy; Nanomaterials; Tumor immunotherapy; Target Block; Drug Carrier

Online: 2 February 2024 (08:46:23 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.1304.v1

Tissue-Based Biomarkers in Aggressive Variant Prostate Cancer: A Narrative Review

Olga Kouroukli, Vasiliki Bravou, Konstantinos Giannitsas, Vasiliki Tzelepi

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: AVPC; biomarkers; NEPC; transdifferentiation; ADT; tumor suppressors; DDR; epigenetic regulation

Online: 17 January 2024 (11:29:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0758.v1

Correlation Between Tumor Differentiation and Biomarkers in Hepatocellular Carcinoma: Implications for Early Diagnosis and Treatment

Mr Tufael, Auditi kar, Vijay Jagdish Upadhye, Atiqur Rahman Sunny, Antonio Raposo, Md. Saidul Islam, Md Haroon Or Rashid, Mohammad Anowar Hussen, Heesup Han, Henrique Douglas Melo Coutinho, Md. Sefaul Ullah, Md. Mizanur Rahman

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Hepatocellular carcinoma; Tumor markers; Chemiluminescence assay; Occupational exposure; Early diagnosis

Online: 10 January 2024 (05:44:27 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.0843.v1

Lipid Nanoparticle (LNP) Delivery Vector-Assisted Targeted Controlled Release mRNA Vaccines in Tumor Immunity

Liusheng Wu, Xiaoqiang Li, Xinye Qian, Shuang Wang, Jixian Liu, Jun Yan

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Lipid nanoparticles (LNPs); mRNA vaccine; Tumor immunity; Delivery vector; Review

Online: 13 December 2023 (03:35:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0357.v1

Carcinogenic Effect of Human Tumors-Derived Cell Free-Filtrates in Nude Mice

Jorge Berlanga-Acosta, Ernesto Arteaga-Hernandez, Ariana Garcia-Ojalvo, Marisol Rodriguez-Touseiro, Dayanis Duvergel-Calderin, Jose Suarez-Alba, Dasha Fuentes-Morales, Osmany Mendoza-Fuentes, Laura Lopez-Marín, Gerardo Guillen-Nieto

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cells-free filtrate; tumor homogenate; cancer; nude mice; malignant transformation

Online: 7 November 2023 (07:07:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1343.v1

Potential of Alkalization Therapy for the Management of Metastatic Pancreatic Cancer: A Retrospective Study

Masahide Isowa, Reo Hamaguchi, Ryoko Narui, Hiromasa Morikawa, Toshihiro Okamoto, Hiromi Wada

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Pancreatic cancer; Cancer metabolism; Alkalization therapy; Tumor microenvironment; Urine pH

Online: 20 October 2023 (11:44:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0583.v1

C-met+ CTLs Exhibit Enhanced Cytotoxicity in Mouse and Human In Vitro Tumor Models

Mahdia Benkhoucha, Ngoc Lan Tran, Isis Senoner, Gautier Breville, Hajer Fritah, Denis Migliorini, Valérie Dutoit, Patrice H. Lalive

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: c-Met; HGF; CTLs; CD8+ cytotoxic T lymphocytes; spheroids; tumor

Online: 10 October 2023 (08:09:05 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.0846.v1

Rhabdomyosarcoma: Current Therapy, Challenges, and Future Approaches to Treatment Strategies

Ali Zarrabi, David Perrin, Mahboubeh Kavoosi, Micah Sommer, Serap Sezen, Parvaneh Mehrbod, Bhavya Bhushan, Filip Machaj, Jakub Rosik, Philip Kawalec, Saba Afifi, Seyed Mohammadreza Bolandi, Peyman Koleini, Mohsen Taheri, Tayyebeh Madrakian, Marek Jan Łos, Benjamin Lindsey, Nilufer Cakir, Atefeh Zarepour, Kiavash Hushmandi, Ali Fallah, Bahattin Koc, Mazaher Ahmadi, Susan E. Logue, Gorka Orive, Stevan Pecic, Joseph W. Gordon, Saeid Ghavami

Subject: Biology And Life Sciences, Life Sciences Keywords: Alveolar Rhabdomyosarcoma; Apoptosis; Autophagy; Unfolded Protein Response; Bioengineering; Tumor Stiffness

Online: 13 September 2023 (10:32:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.2123.v1

Effects of Moderate Exercise Training on Cancer-Induced Muscle Wasting

Ana Cristina Corrêa Figueira, Ana Pereira, Luis Leitão, Rita Ferreira, Paula Oliveira, José Duarte

Subject: Public Health And Healthcare, Physical Therapy, Sports Therapy And Rehabilitation Keywords: Breast tumor; exercise training; gastrocnemius; soleus; cancer-induced muscle wasting

Online: 31 August 2023 (09:36:52 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.1800.v2

Angiogenesis in Lung Cancer: Understanding the Roles of Growth Factors

Yvan Sinclair Ngaha Tchawe, Zhilenkova Angelina V., Essogmo Freddy Elad, Ikenna K. Uchendu, Abah Moses Owoicho, Lionel Tabola Fossa, Zaiana D. Sangadzhieva, Varvara D. Sanikovich, Alexander S. Rusanov, Yuliya N. Pirogova, Alexander Boroda, Alexander Rozhkov, Jean D. Kemfang Ngowa, Leonid N. Bagmet, Marina I. Sekacheva

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Angiogenesis; Growth factors; Lung cancer; Tumor microenvironment; Anti-angiogenic therapy

Online: 29 August 2023 (09:24:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1462.v1

Current Challenges and Future Directions in the Management of Inflammatory Breast Cancer: A Review

Paolo Izzo, Luciano Izzo, Andrea Polistena, Simone Sibio, Massimo Codacci-Pisanelli, Daniele Crocetti, Paolo Meloni, Pierfrancesco Di Cello, Claudia De Intinis, Sara Izzo

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: inflammatory breast cancer (IBC); review; immunotherapy; targeted therapy; tumor microenvironment

Online: 21 July 2023 (12:39:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1767.v1

Usefulness of Tumor Marker Score for Prediction of Prognosis in Hepatocellular Carcinoma Patients Treated with Atezolizumab plus Bevacizumab: A Multicenter Retrospective Study

Kazunari Tanaka, Kunihiko Tsuji, Atsushi Hiraoka, Toshifumi Tada, Masashi Hirooka, Kazuya Kariyama, Joji Tani, Masanori Atsukawa, Koichi Takaguchi, Ei Itobayashi, Shinya Fukunishi, Toru Ishikawa, Kazuto Tajiri, Hironori Ochi, Hidenori Toyoda, Chikara Ogawa, Takashi Nishimura, Takeshi Hatanaka, Satoru Kakizaki, Noritomo Shimada, Kazuhito Kawata, Atsushi Naganuma, Hisashi Kosaka, Tomomitsu Matono, Hidekatsu Kuroda, Yutaka Yata, Hideko Ohama, Fujimasa Tada, Kazuhiro Nouso, Asahiro Morishita, Akemi Tsutsui, Takuya Nagano, Norio Itokawa, Tomomi Okubo, Taeang Arai, Keisuke Yokohama, Hiroki Nishikawa, Michitaka Imai, Yohei Koizumi, Shinichiro Nakamura, Hiroko Iijima, Masaki Kaibori, Yoichi Hiasa, Takashi Kumada

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: hepatocellular carcinoma; tumor marker; atezolizumab plus bevacizumab; prognosis; predictive model

Online: 26 June 2023 (09:42:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1305.v1

Heat Shock Proteins Mediate Intercellular Communications within Tumor Microenvironment Cells through Extracellular Vesicles

Renata F. Saito, Maria Cristina Rangel, Andreia Hanada Otake, Camila Maria Longo Machado

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Heat-shock-protein; extracellular vesicles; reprograming tumors; tumor evolucionary pressure

Online: 18 May 2023 (08:46:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1304.v1

Molecular Networks of Platinum Drugs and Arsenic Trioxide and Its Interaction With microRNAs in Cancer

Shihori Tanabe, Eger Boonstra, Taehun Hong, Sabina Quader, Ryuichi Ono, Horacio Cabral, Kazuhiko Aoyagi, Hiroshi Yokozaki, Hiroki Sasaki

Subject: Biology And Life Sciences, Life Sciences Keywords: drug resistance; cisplatin; arsenic; tumor microenvironment; microRNA; molecular pathway network

Online: 18 May 2023 (08:41:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0705.v1

Contrast-enhanced Liver MR Synthesis using Gradient Regularized Multi-Modal Multi-Discrimination Sparse Attention Fusion GAN

Changzhe Jiao, Diane Ling, Shelly Bian, April Vassantachart, Karen Cheng, Shahil Mehta, Derrick Lock, Zhenyu Zhu, Mary Feng, Horatio Thomas, Jessica E Scholey, Ke Sheng, Zhaoyang Fan, Wensha Yang

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: MR synthesis, GAN, multi-modal fusion, tumor monitoring, contrast enhancement

Online: 10 May 2023 (08:10:11 CEST)

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Purposes: To provide abdominal contrast-enhanced MR image synthesis, we developed an image gradient regularized multi-modal multi-discrimination sparse-attention fusion generative adversarial network (GRMM-GAN) to avoid repeated contrast injections to patients and facilitate adaptive monitoring. Methods: With IRB approval, 165 abdominal MR studies from 61 liver cancer patients were retrospectively solicited from our institutional database. Each study included T2, T1 pre-contrast (T1pre), and T1 contrast-enhanced (T1ce) images. The GRMM-GAN synthesis pipeline consists of a sparse attention fusion network, an image gradient regularizer (GR), and a generative adversarial network with multi-discrimination. The studies were randomly divided into 115 for training, 20 for validation, and 30 for testing. The two pre-contrast MR modalities, T2 and T1pre images, were adopted as inputs in the training phase. The T1ce image at the portal venous phase was used as an output. The synthesized T1ce images were compared with the ground truth T1ce images. The evaluation metrics include peak signal-to-noise ratio (PSNR), structural-similarity-index (SSIM), and mean-squared-error (MSE). A Turing test and experts’ contours evaluated the image synthesis quality. Results: The proposed GRMM-GAN model achieved a PSNR of 28.56, an SSIM of 0.869, and an MSE of 83.27. The proposed model showed statistically significant improvements in all metrics tested with p-values <0.05 over the state-of-the-art model comparisons. The average Turing test score was 52.33%, close to random guessing, supporting the model's effectiveness for clinical application. In the tumor-specific region analysis, the average tumor contrast-to-noise ratio (CNR) of the synthesized MR images was not statistically significant from the real MR images. The average DICE from real vs. synthetic images was 0.90, compared to the inter-operator DICE of 0.91. Conclusion: We demonstrated the function of a novel multi-modal MR image synthesis neural network, GRMM-GAN, for T1ce MR synthesis based on pre-contrast T1 and T2 MR images. GRMM-GAN shows promise for avoiding repeated contrast injections during radiation therapy treatment.

Preprint REVIEW | doi:10.20944/preprints202304.0315.v1

Current Status and De Novo Synthesis of Anti-Tumor Alkaloids in Nicotiana: A Mini-Review

Md. Ahsan Habib, Md. Mobinul Islam, Md. Mukul Islam, Md. Mohidul Hasan, Kwang-Hyun Baek

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Nicotiana; alkaloids; anti-tumor; metabolic engineering; de-novo synthesis ;

Online: 13 April 2023 (08:53:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0236.v1

BAP1 Loss Promotes Suppressive Tumor Immune Microenvironment via Up-Regulation of PROS1 in Class 2 Uveal Melanomas

Christopher J. Kaler, James J. Dollar, Anthony M. Cruz, Jeffim N. Kuznetsoff, Margaret I. Sanchez, Christina L. Decatur, Jonathan D. Licht, Keiran S.M. Smalley, Zelia M. Correa, Stefan Kurtenbach, J. William Harbour

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: uveal melanoma; BAP1; PROS1; MERTK; macrophage; tumor immune microenvironment; metastasis

Online: 16 June 2022 (10:42:50 CEST)

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Uveal melanoma (UM) is the most common primary cancer of the eye and is associated with a high rate of metastatic death. UM can be stratified into two main classes based on metastatic risk, with class 1 UM having a low metastatic risk and class 2 UM having a high metastatic risk. Class 2 UM have a distinctive genomic, transcriptomic, histopathologic, and clinical phenotype characterized by biallelic inactivation of the BAP1 tumor suppressor gene, an immune suppressive microenvironment enriched for M2-polarized macrophages, and poor response to checkpoint inhibitor immunotherapy. To identify potential mechanistic links between BAP1 loss and immune suppression in class 2 UM, we performed an integrated analysis of UM samples, as well as genetically engineered UM cell lines and uveal melanocytes (UMC). Using RNA sequencing (RNA-seq), we found that the most highly up-regulated gene associated with BAP1 loss across these datasets was PROS1, which encodes a ligand that triggers phosphorylation and activation of the immunosuppressive macrophage receptor MERTK. The inverse association between BAP1 and PROS1 in class 2 UM was confirmed by single-cell RNA-seq, which also revealed that MERTK was up-regulated in CD163+ macrophages in class 2 UM. Using ChIP-seq, BAP1 knockdown in UM cells resulted in an accumulation of H3K27ac at the PROS1 locus, suggesting epigenetic regulation of PROS1 by BAP1. Phosphorylation of MERTK in RAW 264.7 monocyte-macrophage cells was increased upon co-culture with BAP1-/- UMCs, and this phosphorylation was blocked by depletion of PROS1 in the UMCs. These findings were corroborated by multi-color immunohistochemistry, where class 2/BAP1-mutant UMs demonstrated increased PROS1 expression in tumor cells and increased MERTK phosphorylation in CD163+ macrophages compared to class 1/BAP1-wildtype UMs. Taken together, these findings provide a mechanistic link between BAP1 loss and suppression of the tumor immune microenvironment in class 2 UMs, and they implicate the PROS1-MERTK pathway as a potential target for immunotherapy in UM.

Preprint REVIEW | doi:10.20944/preprints202206.0167.v1

Convolutional Neural Network Techniques for Brain Tumor Classification (from 2015 to 2021): Review, Challenges, and Future Perspectives

Yuting Xie, Fulvio Zaccagna, Leonardo Rundo, Claudia Testa, Raffaele Agati, Raffaele Lodi, David Neil Manners, Caterina Tonon

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: deep learning; convolutional neural network; brain tumor classification; clinical application

Online: 13 June 2022 (04:57:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202201.0359.v1

Design, Synthesis and Structure-activity Relationship Studies of Meridianin Derivatives as Novel JAK/STAT3 Signaling Inhibitors

JianQiang Zhang, Rui Li, XueYang Dong, Na He, RuiJuan Yin, MengKe Yang, JieYu Liu, RiLei Yu, ChenYang Zhao, Tao Jiang

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: JAK/STAT3 signaling pathway; Meridianin derivatives; Isothiouronium; Anti-tumor activity

Online: 24 January 2022 (14:06:38 CET)

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Preprint REVIEW | doi:10.20944/preprints202110.0368.v1

Therapeutic Applications of Engineered Chimeric Antigen Receptors-T cell for Cancer Therapy

Amina Hussain

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: chimeric antigen receptors-T cell; solid tumor microenvironment; cancer therapy

Online: 25 October 2021 (15:48:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202110.0196.v1

Folic Acid Decorated β-Cyclodextrin-Based Poly(ε-Caprolactone)/Dextran Star Polymer with Disulﬁde Bond-Linker as Theranostic Nanoparticle for Tumor-Targeted MRI and Chemotherapy

Huikang Yang, Nianhua Wang, Ruimeng Yang, Li-Ming Zhang, Xinqing Jiang

Subject: Chemistry And Materials Science, Biomaterials Keywords: star polymer; β-cyclodextrin; tumor-targeted; disulﬁde bond; theranostic nanoparticles

Online: 13 October 2021 (11:00:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0105.v1

The Role of Wnt/Beta-Catenin Pathway, SATB1 and SATB2 in Endometrial Cancer Patients

Paweł Sadłecki, Magdalena Bodnar, Andrzej Marszalek, Malgorzata Walentowicz-Sadlecka

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: endometrial cancer; tumor microenvironment; Wnt/β-catenin pathway; SATB1; SATB2

Online: 6 September 2021 (14:29:35 CEST)

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Wnt/β-catenin signaling pathway plays an established role in various diseases and is considered a hallmark of endometrial cancer (EC). Special AT-rich sequence-binding protein 1 and 2 (SATB1 and SATB2) are nuclear matrix-associated proteins involved in chromatin remodeling and regulation of gene expression. SATB1 promotes the progression of numerous types of cancers, whereas SATB2 acts as a tumor suppressor. Despite a recent progress in our knowledge about EC, the exact mechanisms that control their proliferation and metastatic potential still remain unknown. The aim of our study was to investigate the association between Wnt3A, β-catenin, SATB1 and SATB2 protein level and the clinicopathological features of EC patients. 92 EC patients, aged 37-84, were enrolled to our study. The immunoexpression of WNT3A was found in specimens from all EC patients, β-catenin was expressed in 97% of the cases, SATB1 in 87%. The significant association between Wnt3a expression and tumor grade was found; moreover mean IRS for Wnt3a turned out to be significantly lower in high-grade tumors than in low-grade malignancies (p=0.038). In turn, immunoexpression of β-catenin varied significantly across FIGO stages and was associated with the presence of lymph node metastases. Mean IRS for β-catenin in patients with lymph node metastases was significantly lower than in those without (p = 0.028). The Kaplan-Meier analyses demonstrated a stepwise impairment of cancer overall survival with increasing SATB1 expression. In conclusion, both Wnt/β-catenin signaling pathway and SATB1 contribute to progression of EC. Downregulation of β-catenin may predispose to lymphatic spread of EC. In turn, downregulation of Wnt3a seems to be characteristic for high-grade tumors, but probably does not play a role in formation of lymph node metastases. The important role of SATB1 as a predictor of poor survival and could be helpful in establishing a more accurate prognosis in endometrial cancer patients.

Preprint REVIEW | doi:10.20944/preprints202107.0592.v1

Development of Anticancer Drugs: From Mechanical Properties of Tumor Stiffness to Cytoskeleton-Targeting Natural Products

Xiaoye Liu, Hongwei Xu, Yuting Feng, Xiaolin Hou

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: anticancer drugs; mechanical microenvironment; tumor stiffness; cytoskeleton dynamics; material approaches

Online: 26 July 2021 (15:42:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202105.0779.v1

Lineage Plasticity in Cancer: The Tale of a Skin-walker

Archana P. Thankamony, Ayalur Raghu Subbalakshmi, Mohit Kumar Jolly, Radhika Nair

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Lineage Plasticity; Tumor Progression; Metastasis; Therapy Resistance; Epithelial-Mesenchymal Plasticity

Online: 31 May 2021 (13:48:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202105.0513.v1

Cell Penetrating Peptides: Applications in Tumor Diagnosis and Therapeutics

Jeffrey William Stiltner, Kayla Anne McCandless, Maliha Zahid

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cell penetrating peptides; protein transduction domains; tumor imaging; targeted therapies.

Online: 21 May 2021 (09:44:48 CEST)

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Since their identification over twenty-five years ago, the plethora of cell penetrating peptides (CPP) and their applications has skyrocketed. These 5 to 30 amino acid long peptides have the unique property of breaching the cell membrane barrier while carrying cargoes larger then themselves into cells in an intact, functional form. CPPs can be conjugated to fluorophores, activatable probes, radioisotopes or contrast agents for imaging tissues, such as tumors. There is no singular mechanism for translocation of CPPs into a cell, and therefore, many CPPs are taken up by a multitude of cell types, creating the challenge of tumor specific translocation and hindering clinical effectiveness. Varying strategies have been developed to combat this issue and enhance their diagnostic potential by derivatizing CPPs for better targeting by constructing specific cell activated forms. These methods are currently being used to image integrin expressing tumors, breast cancer cells, human histiocytic lymphoma and protease secreting fibrosarcoma cells, to name a few. Additionally, identifying safe, effective therapeutics for malignant tumors has long been an active area of research. CPPs can circumvent many of the complications found in treating cancer with conventional therapeutics by targeted delivery of drugs into tumors, thereby decreasing off-target side effects, a feat not achievable by currently employed conventional chemotherapeutics. Myriad types of chemotherapeutics such as tyrosine kinase inhibitors, anti-tumor antibodies and nanoparticles can be functionally attached to these peptides leading to the possibility of delivering established and novel cancer therapeutics directly to tumor tissue. While much research is needed to overcome potential issues with these peptides, they offer a significant advancement over current mechanisms to treat cancer. In this review, we present a brief overview of the research leading to identification of CPPs with a comprehensive state of the art review on the role of these novel peptides in both cancer diagnostics as well as therapeutics.

Preprint REVIEW | doi:10.20944/preprints202010.0338.v1

Treatment Strategies and Metabolic Pathway Regulation in Urothelial Cell Carcinoma: A Comprehensive Review

Huang-Yu Yang, Chao-Yi Wu, Jia-Jin Chen, Tao-Ha Lee

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Urothelial carcinoma; immune checkpoint inhibitors; immunotherapy; tumor microenvironment; metabolic pathway

Online: 16 October 2020 (08:00:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0239.v1

Prognostic Value of the Intratumoral Lymphocyte to Monocyte Ratio and M0 Macrophage Enrichment in the Melanoma Immune Microenvironment

Neil Jairath, Mark Farha, Ruple Jairath, Paul Harms, Lam Tsoi, Trilokraj Tejasvi

Subject: Medicine And Pharmacology, Dermatology Keywords: Cutaneous Melanoma; Immunotherapy; Lymphocytes; Monocytes; Macrophages; RNAseq; tumor immune microenvironment

Online: 11 September 2020 (04:02:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202005.0212.v1

Selective Cytotoxic Effects of Annona Squamosa Leaves against Breast Cancer Cells via Apoptotic Signaling Proteins

Rawan Al-Nemari, Abir Ben Bacha, Abdulrahman Al-Senaidy, Maha Arafah, Hadel Al-Saran, Nael Abutaha, Abdelhabib Semlali

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Annona squamosa; phytomedicine; proliferation; apoptosis; migration; estrogen receptor; tumor size

Online: 12 May 2020 (12:37:43 CEST)

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Working Paper REVIEW

Commercial and Emerging Technologies for Cancer Diagnosis and Prognosis Based on Exosomal Biomarkers

Nareg Ohannesian, Dilani Gunawardhana, Ibrahim Misbah, Mohsen Rakhshandehroo, Steven H. Lin, Wei-Chuan Shih

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: circulating tumor biomarkers; extracellular vesicles; biological nanoparticles; liquid biopsy; biosensing

Online: 31 January 2020 (05:18:15 CET)

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Preprint ESSAY | doi:10.20944/preprints201910.0207.v1

Early Detection is as Important as Imatinib in CML Treatment Success

Dmitriy Sonkin, Richard Simon

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: CML; Imatinib; early detection; cancer; tumor progression; oncology; Gleevec; Glivec

Online: 18 October 2019 (07:18:47 CEST)

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Preprint REVIEW | doi:10.20944/preprints201908.0038.v1

STAT3 and STAT5 Activation in Solid Cancers

Sebastian Igelmann, Heidi A. Neubauer, Gerardo Ferbeyre

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: solid cancers; cell cycle; apoptosis; inflammation; mitochondria; stemness; tumor suppression

Online: 5 August 2019 (03:43:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints201705.0127.v1

Aberrant DNA Methylation in Cholangiocarcinoma

Toshiaki Nakaoka, Yoshimasa Saito, Hidetsugu Saito

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cholangiocarcinoma; DNA methylation; Tumor suppressor gene; microRNA; DNA methylation inhibitor

Online: 16 May 2017 (18:08:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0819.v1

Understanding Macrophage Complexity in Metabolic Dysfunction-associated Steatotic Liver Disease: Transitioning from the M1/M2 Paradigm to Spatial Dynamics

Forkan Ahamed, Natalie Eppler, Elizabeth Jones, Yuxia Zhang

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: macrophages; diversity; Kupffer cell; monocyte-derived macrophage; spatial dynamics; metabolic dysfunction-associated steatotic liver disease (MASLD)

Online: 12 April 2024 (04:44:38 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202401.0403.v1

Exosomes from Adipose Tissue-Derived Stem Cells Induce Proapoptotic Gene Expression in Breast Tumor Cell Line

Oliver Felthaus, Simon Vedlin, Andreas Eigenberger, Silvan M. Klein, Lukas Prantl

Subject: Biology And Life Sciences, Life Sciences Keywords: breast cancer; mammary carcinoma; lipofilling; autologous fat grafting; adipose tissue-derived stem cells; exosomes; extracellular vesicles

Online: 4 January 2024 (15:44:45 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.2257.v1

When Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants

Adeeb Rahman, Neeti Sanan-Mishra

Subject: Biology And Life Sciences, Plant Sciences Keywords: Pathogen Derived Resistance; RNA silencing; artificial miRNA; tasiRNA; Epigenetic editing; prime editing; Multiplexing CRISPR/Cas editing

Online: 29 December 2023 (05:55:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1155.v1

Extracellular Matrix Tunes the Regenerative Potential of Fetal Stem Cells

Yixuan Amy Pei, Jhanvee Patel, Ming Pei

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: fetal stem cells; decellularized extracellular matrix; differentiation; fetal nucleus pulposus cell; fetal synovium-derived stem cell

Online: 15 December 2023 (09:22:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0865.v1

Immunohistochemistry Evidence of Platelet-Rich Fibrin to Accelerate Differentiation of Adipose-derived Stem Cells

I Gde Rurus Suryawan, Andrianto Andrianto, Arisya Agita, Anudya Kartika Ratri, Ricardo Adrian Nugraha

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: adipocyte-derived mesenchymal stem cells; cardiomyocyte-like cells; platelet rich fibrin; growth factor; stem cell therapy

Online: 10 August 2023 (13:18:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0444.v1

In Vivo Models for Prostate Cancer Research

Robert Adamiecki, Anita Hryniewicz-Jankowska, Maria A. Ortiz, Xiang Li, Baylee A Porter-Hansen, Imad Nsouli, Gennady Bratslavsky, Leszek Kotula

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: prostate cancer; knockout mouse models; genetically-engineered mouse models; xenografts; patient derived xenografts; organoids; signaling pathways

Online: 26 August 2022 (04:16:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0070.v1

Regenerative Potential of Injectable Platelet-Rich Fibrin to Accelerate Differentiation of Adipose-derived Mesenchymal Stem Cells into Cardiomyocytes-like Cells

I Gde Rurus Suryawan, Andrianto Andrianto, Arisya Agita, Anudya Kartika Ratri, Ricardo Adrian Nugraha

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: adipocyte-derived mesenchymal stem cells; cardiomyocyte-like cells; platelet rich fibrin; growth factor; stem cell therapy

Online: 3 August 2022 (03:31:51 CEST)

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Background: There is several challenges to solve irreversible loss of cardiomyocytes due to myocardial infarction. Cell therapy is believed as an ideal treatment for cardiac regeneration in the infarct area. Obtaining adipose-derived stem cells increases seems to be promising, however it is limited by the capacity to differentiate. Stimulation by injectable platelet-rich fibrin appears to have the beneficial effects to accelerate cardiomyocyte-like cells differentiation. Objective: To analyse the benefit of injectable platelet-rich fibrin to accelerate differentiation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells. Methods: This study is a true experimental randomized post-test design study. Adipose-derived mesenchymal stem cells were isolated from adipose tissues and cultured until 4 passages. The characteristics of adipose-derived mesenchymal stem cells were measured by the expression of CD 34-, CD 45-, and CD 105+ using flowcytometry. The samples were divided into 3 groups, i.e. negative control (α-MEM), positive control (differentiation medium) and treatment group (platelet-rich fibrin). The assessment of GATA-4 marker expression was conducted using flowcytometry on the fifth day and troponin was conducted using immunocytochemistry on the tenth day to determine the differentiation to cardiomyocyte. Data analysis was conducted using T-test and One-Way ANOVA on normally distributed data determined through Shapiro-Wilk test. Results: Flowcytometry on GATA-4 expression revealed significant difference on addition of platelet-rich fibrin compared with negative and positive controls (68.20 ± 6.82 vs 58.15 ± 1.23; p<0.05; 68.20 ± 6.82 vs 52.96 ± 2.02; p<0.05). This was supported by the results of immunocytochemistry on troponin expression which revealed significant difference between platelet-rich fibrin group compared with negative and positive controls (50.66 ± 7.2 vs 10.73 ± 2.39; p<0.05; 50.66 ± 7.2 vs 26.00 ± 0.4; p<0.05). Conclusion: Injectable platelet-rich fibrin has beneficial effect to accelerate differentiation of adipose-derived mesenchymal stem cells into cardiomyocyte-like cells.

Preprint REVIEW | doi:10.20944/preprints202101.0337.v1

Advanced Pediatric Diffuse Pontine Glioma Animal Models Pave the Way Towards Precision Medicine

Zirong Chen, Peng Peng, Xiaolin Zhang, Barbara Mania-Farnell, Guifa Xi, Feng Wan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Diffuse intrinsic pontine glioma; molecular biology; patient derived xenografts; genetically engineered mouse model; humanized mouse model

Online: 18 January 2021 (12:50:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints201911.0162.v1

Characterization Of Human Pluripotent Stem Cell-Derived Hepatocytes With Adult Features And Potential For Modeling Metabolic Diseases

Gustav Holmgren, Benjamin Ulfenborg, Annika Asplund, Karin Toet, Christian X Andersson, Ann Hammarstedt, Roeland Hanemaaijer, Barbara Küppers-Munther, Jane Synnergren

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: human pluripotent stem cells; human stem cell-derived hepatocytes; in vitro; metabolic diseases; transcriptomics; maturation; characterization

Online: 14 November 2019 (11:24:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202405.0962.v1

Effectiveness of a Single Fixed Dose 3 mg Rasburicase for Prevention and Management of Hyperuricemia of Tumor Lysis Syndrome in adult Cancer Patients

Sabirin Bakhsh, Mansoor Ahmed Khan, Majed Alshamrani, Roula Mufti, Anjum Naeem Ansari, Mansour Almubarak, Ahmad Alsaeed, Mousa Alahmari, Muhammad Aseeri

Subject: Oncology And Oncogenics, Medicine And Pharmacology Keywords: Rasburicase; Tumor Lysis syndrome; Cost-effectiveness; 3 mg single fixed dose

Online: 14 May 2024 (12:46:34 CEST)

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