Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Role of Wnt/Beta-Catenin Pathway, SATB1 and SATB2 in Endometrial Cancer Patients

Version 1 : Received: 6 September 2021 / Approved: 6 September 2021 / Online: 6 September 2021 (14:29:35 CEST)

How to cite: Sadłecki, P.; Bodnar, M.; Marszalek, A.; Walentowicz-Sadlecka, M. The Role of Wnt/Beta-Catenin Pathway, SATB1 and SATB2 in Endometrial Cancer Patients. Preprints 2021, 2021090105 (doi: 10.20944/preprints202109.0105.v1). Sadłecki, P.; Bodnar, M.; Marszalek, A.; Walentowicz-Sadlecka, M. The Role of Wnt/Beta-Catenin Pathway, SATB1 and SATB2 in Endometrial Cancer Patients. Preprints 2021, 2021090105 (doi: 10.20944/preprints202109.0105.v1).

Abstract

Wnt/β-catenin signaling pathway plays an established role in various diseases and is considered a hallmark of endometrial cancer (EC). Special AT-rich sequence-binding protein 1 and 2 (SATB1 and SATB2) are nuclear matrix-associated proteins involved in chromatin remodeling and regulation of gene expression. SATB1 promotes the progression of numerous types of cancers, whereas SATB2 acts as a tumor suppressor. Despite a recent progress in our knowledge about EC, the exact mechanisms that control their proliferation and metastatic potential still remain unknown. The aim of our study was to investigate the association between Wnt3A, β-catenin, SATB1 and SATB2 protein level and the clinicopathological features of EC patients. 92 EC patients, aged 37-84, were enrolled to our study. The immunoexpression of WNT3A was found in specimens from all EC patients, β-catenin was expressed in 97% of the cases, SATB1 in 87%. The significant association between Wnt3a expression and tumor grade was found; moreover mean IRS for Wnt3a turned out to be significantly lower in high-grade tumors than in low-grade malignancies (p=0.038). In turn, immunoexpression of β-catenin varied significantly across FIGO stages and was associated with the presence of lymph node metastases. Mean IRS for β-catenin in patients with lymph node metastases was significantly lower than in those without (p = 0.028). The Kaplan-Meier analyses demonstrated a stepwise impairment of cancer overall survival with increasing SATB1 expression. In conclusion, both Wnt/β-catenin signaling pathway and SATB1 contribute to progression of EC. Downregulation of β-catenin may predispose to lymphatic spread of EC. In turn, downregulation of Wnt3a seems to be characteristic for high-grade tumors, but probably does not play a role in formation of lymph node metastases. The important role of SATB1 as a predictor of poor survival and could be helpful in establishing a more accurate prognosis in endometrial cancer patients.

Keywords

endometrial cancer; tumor microenvironment; Wnt/β-catenin pathway; SATB1; SATB2

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.