Suryawan, I.G.R.; Andrianto, A.; Agita, A.; Ratri, A.K.; Nugraha, R.A. Immunohistochemistry Evidence of Platelet-Rich Fibrin to Accelerate Differentiation of Adipose-derived Stem Cells. Preprints2023, 2023080865. https://doi.org/10.20944/preprints202308.0865.v1
APA Style
Suryawan, I.G.R., Andrianto, A., Agita, A., Ratri, A.K., & Nugraha, R.A. (2023). Immunohistochemistry Evidence of Platelet-Rich Fibrin to Accelerate Differentiation of Adipose-derived Stem Cells. Preprints. https://doi.org/10.20944/preprints202308.0865.v1
Chicago/Turabian Style
Suryawan, I.G.R., Anudya Kartika Ratri and Ricardo Adrian Nugraha. 2023 "Immunohistochemistry Evidence of Platelet-Rich Fibrin to Accelerate Differentiation of Adipose-derived Stem Cells" Preprints. https://doi.org/10.20944/preprints202308.0865.v1
Abstract
Background: Obtaining adipose-derived stem cells is limited by its capacity to differentiate. Stimulation by platelet-rich fibrin appears to have the beneficial effects to accelerate differentiation.
Objective: To obtain immunohistochemistry evidence of platelet-rich fibrin to accelerate differentiation of adipose-derived stem cells.
Methods: We used post-test only controlled-group design. Adipose-derived stem cells were isolated and cultured until 4 passages in α-MEM culture medium.
Results: GATA-4 expression increased in platelet-rich fibrin group compared with control group (68.20±6.82 vs 58.15±1.23 counts; p<0.05; 68.20±6.82 vs 52.96±2.02 counts; p<0.05). Troponin expression also increased in platelet-rich fibrin group (50.66±7.2 vs 10.73±2.39 counts; p<0.05; 50.66±7.2 vs 26.00±0.4 counts; p<0.05).
Conclusion: There is an immunohistochemistry benefit of platelet-rich fibrin to accelerate differentiation of adipose-derived stem cells into cardiomyocyte-like cells.
Medicine and Pharmacology, Cardiac and Cardiovascular Systems
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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