Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Immunohistochemistry Evidence of Platelet-Rich Fibrin to Accelerate Differentiation of Adipose-derived Stem Cells

Version 1 : Received: 9 August 2023 / Approved: 10 August 2023 / Online: 10 August 2023 (13:18:57 CEST)

A peer-reviewed article of this Preprint also exists.

I Gde Rurus Suryawa, Andrianto, Arisya Agita, Anudya Kartika Ratri and Ricardo Adrian Nugraha. Emerging therapeutic benefit of platelet-rich fibrin as novel platelet concentrates in tissue engineering. Global Cardiology Science & Practice. Vol. 2024 No. 5,  DOI: https://doi.org/10.21542/gcsp.2024.46 
I Gde Rurus Suryawa, Andrianto, Arisya Agita, Anudya Kartika Ratri and Ricardo Adrian Nugraha. Emerging therapeutic benefit of platelet-rich fibrin as novel platelet concentrates in tissue engineering. Global Cardiology Science & Practice. Vol. 2024 No. 5,  DOI: https://doi.org/10.21542/gcsp.2024.46 

Abstract

Background: Obtaining adipose-derived stem cells is limited by its capacity to differentiate. Stimulation by platelet-rich fibrin appears to have the beneficial effects to accelerate differentiation. Objective: To obtain immunohistochemistry evidence of platelet-rich fibrin to accelerate differentiation of adipose-derived stem cells. Methods: We used post-test only controlled-group design. Adipose-derived stem cells were isolated and cultured until 4 passages in α-MEM culture medium. Results: GATA-4 expression increased in platelet-rich fibrin group compared with control group (68.20±6.82 vs 58.15±1.23 counts; p<0.05; 68.20±6.82 vs 52.96±2.02 counts; p<0.05). Troponin expression also increased in platelet-rich fibrin group (50.66±7.2 vs 10.73±2.39 counts; p<0.05; 50.66±7.2 vs 26.00±0.4 counts; p<0.05). Conclusion: There is an immunohistochemistry benefit of platelet-rich fibrin to accelerate differentiation of adipose-derived stem cells into cardiomyocyte-like cells.

Keywords

adipocyte-derived mesenchymal stem cells; cardiomyocyte-like cells; platelet rich fibrin; growth factor; stem cell therapy

Subject

Medicine and Pharmacology, Cardiac and Cardiovascular Systems

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