Preprint Article Version 1 This version is not peer-reviewed

Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy

Version 1 : Received: 2 May 2018 / Approved: 3 May 2018 / Online: 3 May 2018 (12:16:15 CEST)

A peer-reviewed article of this Preprint also exists.

Chang, J.-E.; Liu, Y.; Lee, T.H.; Lee, W.K.; Yoon, I.; Kim, K. Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy. Int. J. Mol. Sci. 2018, 19, 1596. Chang, J.-E.; Liu, Y.; Lee, T.H.; Lee, W.K.; Yoon, I.; Kim, K. Tumor Size-Dependent Anticancer Efficacy of Chlorin Derivatives for Photodynamic Therapy. Int. J. Mol. Sci. 2018, 19, 1596.

Journal reference: Int. J. Mol. Sci. 2018, 19, 1596
DOI: 10.3390/ijms19061596

Abstract

Photodynamic therapy (PDT) with a suitable photosensitizer molecule is a promising anticancer treatment. We evaluated two chlorin molecules as potential photosensitizers, methyl pyropheophorbide a (MPPa) and N-methoxyl purpurinimide (NMPi), against A549 human lung adenocarcinoma cells in vitro as well as in A549 tumor-bearing mice in vivo. Cell viability, microscopy, and FACS analyses were performed for the in vitro studies. MPPa and NMPi showed high phototoxicity in vitro, which was dependent on the concentration of the photosensitizers as well as the light irradiation time. In the animal study, tumor volume change, tumor surface alterations, and H&E and TUNEL staining analyses were performed and compared between small (tumor volume of <50 mm3) and large (tumor volume of >50 mm3) size of initial tumors. MPPa and NMPi showed high anticancer efficacy against small-size tumors, indicating that early treatment with PDT is effective. Especially, repeated two times PDT with NMPi allowed almost complete eradication against small-size tumors. However, MPPa and NMPi were not effective against large-size tumors. In conclusion, the two chlorin derivatives, MPPa and NMPi, show good anticancer efficacy as promising photosensitizers for PDT in vitro and in vivo. Moreover, their activity in vivo was significantly dependent on the initial tumor size in mice, which confirms the importance of early cancer treatment.

Subject Areas

cancer treatment; chlorin; Photodynamic therapy (PDT); photosensitizer; tumor size

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