Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

When Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants

Version 1 : Received: 28 December 2023 / Approved: 29 December 2023 / Online: 29 December 2023 (05:55:13 CET)

A peer-reviewed article of this Preprint also exists.

Rahman, A.; Sanan-Mishra, N. When an Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants. Agriculture 2024, 14, 282. Rahman, A.; Sanan-Mishra, N. When an Intruder Comes Home: GM and GE Strategies to Combat Virus Infection in Plants. Agriculture 2024, 14, 282.

Abstract

Viruses are silent enemies that intrude and take control of the plant cell's machinery for their own multiplication. Infection by viruses and the resulting damage is still a major challenge in the agriculture sector. Plants have the ability to fight back, but the ability of viruses to counteract and mutate at a fast rate helps them to hide from the host response. Therefore, classical approaches for introgressing resistance genes by breeding have obtained limited success in counteracting the virus menace. Genetic modification (GM) based strategies have been successful in engineering artificial resistance in plants. Several different approaches based on pathogen derived resistance, antisense constructs, hairpin RNA, double stranded RNA etc. have been used to enhance plant resistance to the virus. Recently, genome editing (GE) strategies mainly involving the CRISPR/Cas mediated modifications are being used for virus control. In this review, we discuss the developments and advancements in the GM and GE based methods in tackling virus infection in plants.

Keywords

Pathogen Derived Resistance; RNA silencing; artificial miRNA; tasiRNA; Epigenetic editing; prime editing; Multiplexing CRISPR/Cas editing

Subject

Biology and Life Sciences, Plant Sciences

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