Preprint Article Version 1 This version is not peer-reviewed

Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Antigenotoxicity, Cytotoxicity and Apoptotic Effects

Version 1 : Received: 11 May 2017 / Approved: 11 May 2017 / Online: 11 May 2017 (17:31:47 CEST)

A peer-reviewed article of this Preprint also exists.

Martínez-Valdivieso, D.; Font, R.; Fernández-Bedmar, Z.; Merinas-Amo, T.; Gómez, P.; Alonso-Moraga, Á.; del Río-Celestino, M. Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Anti-Genotoxicity, Cytotoxicity and Apoptotic Effects. Nutrients 2017, 9, 755. Martínez-Valdivieso, D.; Font, R.; Fernández-Bedmar, Z.; Merinas-Amo, T.; Gómez, P.; Alonso-Moraga, Á.; del Río-Celestino, M. Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Anti-Genotoxicity, Cytotoxicity and Apoptotic Effects. Nutrients 2017, 9, 755.

Journal reference: Nutrients 2017, 9, 755
DOI: 10.3390/nu9070755

Abstract

Zucchini (Cucurbita pepo subsp. pepo) is a seasonal vegetable with high nutritional and medical value. Many useful properties of this fruit are attributed to its bioactive compounds. Zucchini fruits (“Yellow” and “Light Green” varieties) and four distinctive components (lutein, β-carotene, zeaxanthin and dehydroascorbic acid)were selected. Firstly it was determinated the lutein, β-carotene, zeaxanthin and dehydroascorbic acid content in these fruits and then, in order to measure the safety and suitability of their use  different assays were carried out: (i) Genotoxicity and antigenotoxicity tests to determine the safety and DNA-protection against hydrogen peroxide; (ii) cytotoxicity and (iii) DNA fragmentation assay to evaluate the proapoptotic effect. Results showed that: (i) all the substances were non-genotoxic, (ii) all the substances were antigenotoxic except the highest concentration of lutein, (iii) “Yellow” Zucchini epicarp and mesocarp exhibited the highest cytotoxic activity (IC50 > 0.1 mg/mL and 0.2 mg/mL, respectively) and iv) “Light Green” Zucchini skin and pulp induced internucleosomal DNA fragmentation . To sump up, Zucchini fruit could play a positive role in human health and nutrition due to this fruit and its components were safe, able to inhibit significantly the H2O2-induced damage and exhibit antiproliferative and pro-apoptotic properties toward HL60 tumour cells. The information generated from this research should take into account to select potential accessions for breeding programs purposes.

Subject Areas

cancer; carotenoid; chemoprevention; Cucurbita; cytotoxicity; DNA-protection; tumor cells

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