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Preprint BRIEF REPORT | doi:10.20944/preprints202104.0658.v1

SCA Medium: A New Culture Medium for the Isolation of all Candida auris Clade

Ahmad Ibrahim, Lucie Peyclit, Rim Abdallah, Saber Khelaifia, Amanda Chamieh, Jean-Marc Rolain, Fadi Bittar

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Candida; Candida auris; Culture; Emerging fungus; Isolation; Specific medium

Online: 26 April 2021 (11:06:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201907.0285.v1

Usefulness of a Concept Called Autonomous Selection

Idan S. Solon

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: extended evolutionary synthesis; inheritance of acquired characters; stress-induced mutagenesis; fitness-dependent sex; horizontal gene transfer

Online: 25 July 2019 (08:24:32 CEST)

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Preprint REVIEW | doi:10.20944/preprints202008.0649.v1

The Origin and Immune Recognition of Tumor-Specific Antigens

Anca Apavaloaei, Marie-Pierre Hardy, Pierre Thibault, Claude Perreault

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: antigen processing and presentation; cancer immunotherapy; cross-priming; immunogenicity; major histocompatibility complex; T lymphocyte; tumor-infiltrating lymphocytes, tumor microenvironment; tumor-specific antigen

Online: 30 August 2020 (10:30:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0213.v1

Independent Evolution of Sex Chromosomes in Eublepharid Geckos, a Lineage with Environmental and Genotypic Sex Determination

Eleonora Pensabene, Lukáš Kratochvíl, Michail Rovatsos

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Gekkota; reptiles; DNA-seq; sex chromosomes; sex determination; qPCR

Online: 5 November 2020 (14:14:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202207.0372.v1

Cell Compartment-specific Folding of Ty1 Long Terminal Repeat Retrotransposon RNA Genome

Małgorzata Zawadzka, Angelika Andrzejewska-Romanowska, Julita Gumna, David J. Garfinkel, Katarzyna Pachulska-Wieczorek

Subject: Biology And Life Sciences, Virology Keywords: RNA genome; RNA structure; Cell compartment-specific folding; LTR-retrotransposon; gRNA dimerization; gRNA cyclization; tRNA annealing; Ty1; Gag.

Online: 25 July 2022 (10:07:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0335.v1

Personalization of a Human Body Model Using Subject-Specific Clothing Industry Dimensions

Luděk Hynčík, Hana Čechová, Tomasz Bońkowski, Gabriela Kavalířová, Petra Špottová, Viola Hampejsová, Hong Meng

Subject: Engineering, Other Keywords: human body; anthropometric dimensions; personalization; subject-specific model; biofidelity

Online: 20 September 2021 (14:15:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201810.0579.v1

Association between Problematic Internet Use and Sleep Disturbance Among Adolescents: the Role of the Child’s Sex

Jiewen Yang, Yangfeng Guo, Xueying Du, Yi Jiang, Wanxin Wang, Di Xiao, Tian Wang, Ciyong Lu, Lan Guo

Subject: Social Sciences, Psychology Keywords: Problematic Internet use, sleep disturbance, sex difference, adolescents

Online: 24 October 2018 (14:15:09 CEST)

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Working Paper ARTICLE

Artiﬁcial Neural Network for Uncertainty Quantiﬁcation in RF Radiation Modeling with High-Dimensional Data

Xi Cheng, Clement Henry, Francesco Andriulli, Christian Person, Joe Wiart

Subject: Computer Science And Mathematics, Mathematics Keywords: artificial neural networks; uncertainty quantification; specific absorption rate

Online: 6 March 2020 (02:57:06 CET)

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Working Paper ARTICLE

Functional Analysis of the Acinetobacter baumannii XerC and XerD Site-Specific Recombinases: Potential Role in Dissemination of Resistance Genes

David L. Lin, German M. Traglia, Rachel Baker, David J. Sherratt, Maria S. Ramirez, Marcelo E. Tolmasky

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: site-specific recombination; carbapenemase; ESKAPE; Acinetobacter; plasmid; Xer; dif; pdif; Re27; gene transfer; gene dissemination; horizontal transfer; horizontal dissemination

Online: 10 July 2020 (02:10:07 CEST)

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Preprint REVIEW | doi:10.20944/preprints202008.0591.v1

Sex Differences in Exercise Induced Bronchoconstriction in Athletes: A Systematic Review and Meta-Analysis

Daniel Rodriguez Bauza, Patricia Silveyra

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: inflammation; atopy; exercise induced asthma; exercise induced bronchoconstriction; sex differences

Online: 27 August 2020 (05:31:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0243.v1

Action and Entropy in Heat Engines: An Action Revision of the Carnot Cycle

Ivan Robert Kennedy, Migdat Hodzic

Subject: Physical Sciences, Quantum Science And Technology Keywords: Carnot cycle; Caloric; specific heat; entropy; Gibbs potential; vortical entropy; reversible cycle; working fluid, quantum field, relative action, heat engine

Online: 11 May 2021 (11:18:39 CEST)

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Despite the remarkable success of Carnot’s heat engine cycle in founding the discipline of thermodynamics two centuries ago, false viewpoints of his use of the caloric theory in the cycle still linger, limiting his legacy. An action revision of the Carnot cycle can correct this, showing that the heat flow powering external mechanical work is compensated internally with configurational changes in the thermodynamic or Gibbs potential of the working fluid, differing in each stage of the cycle quantified by Carnot as caloric. Action (@) is a property of state having the same physical dimensions as angular momentum (mrv=mr2ω). However, this property is scalar rather than vectorial, including a dimensionless phase angle (@=mr2ωδφ). We have recently confirmed with atmospheric gases that their entropy is a logarithmic function of the relative vibrational, rotational and translational action ratios with Planck’s quantum of action ħ. The Carnot principle shows that the maximum rate of work (puissance motrice) possible from the reversible cycle is controlled by the difference in temperature of the hot source and the cold sink, the colder the better. This temperature difference between the source and the sink also controls the isothermal variations of the Gibbs potential of the working fluid, that Carnot identified as reversible temperature-dependent but unequal exchanges in caloric. Importantly, the engine’s inertia ensures that heat from work performed adiabatically in the expansion phase is all restored to the working fluid during the adiabatic recompression, less the net work performed. This allows both the energy and the thermodynamic potential to return to the same values at the beginning of each cycle, a point strongly emphasized by Carnot. Our action revision equates Carnot’s calorique, or the non-sensible heat later described by Clausius as ‘work-heat’ exclusively to negative Gibbs energy (-G) or quantum field energy. This action field complements the sensible energy or vis-viva heat as molecular kinetic motion and its recognition should have significance for designing more efficient heat engines or better understanding of the heat engine powering the Earth’s climates.

Preprint ARTICLE | doi:10.20944/preprints202010.0187.v1

The Impact of Water Loss by Evaporation and Calcite Precipitation on the Sodium Adsorption Ratio (SAR) and an Alternative Method of Estimating the SAR of Irrigation Drainage Water

Qiyu Zhou, William Bleam, Douglas Soldat

Subject: Environmental And Earth Sciences, Water Science And Technology Keywords: sodium adsorption ratio; SAR; CROSS, electrical conductivity; specific conductivity; salinity; irrigation; groundwater; water quality

Online: 9 October 2020 (08:45:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202002.0038.v1

Changes in DNA Methylation in Response to 6-Benzylaminopurine Affect Allele-Specific Gene Expression in Populus tomentosa

Anran Xuan, Yuepeng Song, Chenhao Bu, Panfei Chen, Yousry A. El-Kassaby, Deqiang Zhang

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: allele specific expression; 6-BA; DNA methylation; long noncoding RNA; siRNA; poplar

Online: 4 February 2020 (05:22:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202103.0328.v1

Epitope Mapping of the Diphtheria Toxin and Development of an ELISA-Specific Diagnostic Assay

Salvatore Giovanni De-Simone, Larissa Rodrigues Gomes, Paloma Napoleão-Pêgo, Guilherme Curty Lechuga, Jorge Soares de Pina, Flavio Rocha Silva

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Diphtheria toxin; B epitopes; epitope mapping; bi-specific peptides; ELISA; diagnostic performance

Online: 11 March 2021 (16:43:02 CET)

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Preprint REVIEW | doi:10.20944/preprints202208.0220.v1

Promoting Oncolytic Vector Replication With Switches That Detect Ubiquitous Mutations

Michael Renteln

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Molecular switches; oncolytic vectors; patient-specific ubiquitous mutations; targeted therapy; multi-region sequencing; molecular biology

Online: 11 August 2022 (11:50:12 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.0633.v1

Exploring the Therapeutic Potential of Natural Products on Polycystic Ovarian Syndrome (PCOS): A Mini Review of Lipid Profile, Blood Glucose, and Ovarian Histological Improvements

Syawany Wahid, Muhammad Danial Che Ramli, Ezza Fazleen Mohd Fathil, Muhammad Naim Rosli, Mohd Helmy Mokhtar

Subject: Medicine And Pharmacology, Reproductive Medicine Keywords: natural products; polycystic ovarian syndrome; lipid profile; sex hormone; blood glucose

Online: 11 July 2023 (05:19:58 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202112.0040.v1

The Comparative Analysis of the DNA Repeat Composition among Cannabis Sativa L., Humulus Lupulus L. And Humulus Japonicus Siebold & Zucc. With Heteromorphic Sex Chromosomes

Julia V. Bocharkina, Oleg S. Alexandrov, Olga V. Razumova, Gennady I. Karlov

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cannabis sativa L.; Humulus lupulus L.; Humulus japonicus Siebold & Zucc.; sex chromosomes; DNA repeats; transposons; genome sequencing

Online: 3 December 2021 (09:25:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.0906.v5

Establishment of a Novel Cancer-Specific Anti-HER2 Monoclonal Antibody H2Mab-250/H2CasMab-2 for Breast Cancers

Mika K Kaneko, Hiroyuki Suzuki, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: HER2; cancer-specific monoclonal antibody; screening; epitope; flow cytometry

Online: 4 January 2024 (11:30:55 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0135.v1

Implementation of Environment, Social, and Governance (ESG): Case of Korean Global Firms

Diane So-Hyun Park, Taewon Lee, Hee Kyung Kim, Shawn Seung-Chul Kim

Subject: Business, Economics And Management, Business And Management Keywords: ESG Integration; Korean Multinationals; Sustainability’ Industry-Specific Strategies; Corporate Governance

Online: 3 January 2024 (10:05:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202012.0489.v1

Clinical Significance of Velamentous Cord Insertion Prenatally Diagnosed in Twin Pregnancy

Hyun-mi Lee, SiWon Lee, Min-Kyung Park, You Jung Han, Moon Young Kim, Hye Yeon Boo, Jin Hoon Chung

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Velamentous cord insertion; Twin; Perinatal outcomes; Pregnancy outcomes; Chorionicity; Twin specific complications

Online: 21 December 2020 (10:14:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202104.0583.v1

Free Energies of Hydrated Halide Anions: High throughput Computations on Clusters to Treat Rough Energy-Landscapes

Diego Gomez, Lawrence Pratt, David Rogers, Susan Rempe

Subject: Physical Sciences, Acoustics Keywords: ion hydration; physical cluster theory; halides; Hofmeister series; specific ion effects

Online: 21 April 2021 (13:36:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202108.0309.v1

Gender Based Comparison and Effects of Multi-Year Physical Exercise Program on Motor Skills in Preschool Children

Kristian Plazibat, Josip Karuc, Tihomir Vidranski

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: sex differences; fundamental motor skill; motor skills; physical activity; exercise effects; pediatrics

Online: 14 August 2021 (14:45:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202202.0169.v1

Girls Outperform Boys in Early Syntactic Development? Negative Evidence from Mandarin-Speaking Preschoolers

Nga Yui Tong, Hui Li

Subject: Social Sciences, Language And Linguistics Keywords: sex differences; syntactic development; Mandarin-speaking children

Online: 14 February 2022 (09:19:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202106.0593.v1

Sex-Specific Differences in Toxicity Following Systemic Paclitaxel Treatment and Localized Cardiac Radiotherapy

Nicole Chmielewski Stivers, Benoit Petit, Jonathan Ollivier, Virginie Monceau, Pelagia Tsoutsou, Ana Quintela Pousa, Xiaoming Lin, Charles Limoli, Marie-Catherine Vozenin

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: chemotherapy; radiotherapy; sex-specificity

Online: 24 June 2021 (08:47:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0528.v1

Short-Term Cumulative Exposure to Ambient Traffic-Related Black Carbon and Blood Pressure: MMDA Traffic Enforcers’ Health Study

Zypher Jude G. Regencia, Godofreda V. Dalmacion, Antonio D. Ligsay, Emmanuel S. Baja

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: black carbon; systolic blood pressure; diastolic blood pressure; sex; obesity; traffic enforcers

Online: 30 September 2021 (16:08:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0420.v1

Normative scores for CrossFit® Open Workouts: 2011 - 2022

Gerald T. Mangine, Nina Grundlingh, Yuri Feito

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: fitness assessment; sport-specific; athlete classification; high-intensity functional training; sex differences

Online: 22 December 2022 (06:34:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202202.0310.v1

Translating the Biology of Rodents’ Sex-Recognition and Mate-Recognition to Humans’: Bridging the Pheromonal-Visual Gap

Yehuda Salu

Subject: Social Sciences, Behavior Sciences Keywords: Sex-recognition; mate-recognition; sexual orientation; puberty; vomeronasal-organ; VNO; learning sexual behavior; Medial-geniculate-nucleus; MGN; MASH

Online: 24 February 2022 (10:06:07 CET)

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Working Paper ARTICLE

Comparative Transcriptomics of Non-Embryogenic and Embryogenic Callus in Semi-Recalcitrant and Non-Recalcitrant Upland Cotton Lines

Sonika Kumar, Ashleigh Ruggles, Sam Logan, Alora Mazarakis, Tyson Thomas, Matthew Bates, Dave Reed, Clayton Grosse, Zhigang Li, Jane Grimwood, Jeremy Schmutz, Christopher A Saski

Subject: Biology And Life Sciences, Plant Sciences Keywords: callus; genotype-specific recalcitrance; reprogramming; somatic embryogenesis; transcriptomics; upland cotton

Online: 20 July 2021 (15:30:47 CEST)

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Preprint REVIEW | doi:10.20944/preprints202211.0075.v1

Use of Antiandrogens as Therapeutic Agents in COVID-19 Patients

Efstathios S. Giotis, Emine Cil, Greg N. Brooke

Subject: Biology And Life Sciences, Virology Keywords: coronavirus; TMPRSS2; ACE2; SARS-CoV-2; androgens; sex hormones; antiandrogens

Online: 3 November 2022 (09:21:09 CET)

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Preprint REVIEW | doi:10.20944/preprints202209.0487.v2

Innate Immunity in Cardiovascular Diseases – Identification of Novel Molecular Players and Targets

Wolfgang Poller, Bettina Heidecker, Enrico Ammirati, Andreas W Kuss, Ana Tzvetkova, Wolfram C Poller, Carsten Skurk, Arash Haghikia

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Immunology; innate immunity; immunogenetics; noncoding genome; tRNA biology; evolutionary genetics (list 3-10 specific to the article yet reasonably common within the subject discipline)

Online: 1 November 2022 (09:55:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202001.0051.v1

Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored Poly(A)-Specific Ribonuclease (PARN)

Tian-Li Duan, Han Jiao, Guang-Jun He, Yong-Bin Yan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: deadenylation; DNA damage response; ER-anchored ribonuclease; ER-associated mRNAs; mRNA decay; poly(A) length profile; poly(A)-specific ribonuclease; translation efficiency

Online: 6 January 2020 (02:48:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202109.0110.v1

Effects of Sex and 17β-Estradiol on Cardiac Fibroblast Morphology and Signaling Activities in vitro

Kelsey Watts, Will Richardson

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: cardiac fibroblasts; sex-specific; estrogen; fibrosis; heart failure

Online: 6 September 2021 (17:24:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0234.v1

Alcohol Dependence Induces CRF Sensitivity in Female Central Amygdala GABA Synapses

Larry Rodriguez, Dean Kirson, Sarah A. Wolfe, Reesha R. Patel, Florence P. Varodayan, Angela E. Snyder, Pauravi J. Gandhi, Sophia Khom, Roman Vlkolinksy, Michal Bajo, Marisa Roberto

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: corticotropin releasing factor (CRF); patch-clamp electrophysiology; sex difference; alcohol use disorder (AUD); Gamma-Aminobutyric Acid (GABA); central amygdala (CeA); spontaneous inhibitory post synaptic currents (sIPSCs)

Online: 16 June 2022 (08:41:35 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202212.0219.v1

SARS-CoV-2 Breakthrough Infections after Third Doses Boost IgG Specific Salivary and Blood Antibodies

Maria Noel Badano, Matias Pereson, Florencia Sabbione, Irene Keitelman, Natalia Aloisi, Roberto Chuit, Maria Marta Elizalde de Bracco, Susana Fink, Patricia Bare

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: SARS-CoV-2 breakthrough infections; vaccines; anti-spike antibody levels; humoral response; IgG specific salivary antibodies

Online: 13 December 2022 (02:28:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202105.0457.v1

Body Mass Index and Birth Weight Improve Polygenic Risk Score for Type 2 Diabetes

Avigail Moldovan, Yedael Y. Waldman, Nadav Brandes, Michal Linial

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Body weight; Genetic variations; GWAS; Metabolic disease; Obesity; Sex difference; UK-Biobank

Online: 20 May 2021 (08:55:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202107.0635.v1

Genetic and Epigenetic Factors of Takotsubo Syndrome: a Systematic Review

Valentina Ferradini, Davide Vacca, Beatrice Belmonte, Ruggiero Mango, Giuseppe Novelli, Carmela Rita Balistreri, Federica Carla Sangiuolo

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Takotsubo cardiomyopathy (TTS); biomarkers; specific and effective treatments; TTS management; genetic and epigenetic factors; systematic review

Online: 28 July 2021 (17:07:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0152.v1

Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Massimo Sangiorgi, Mariangela Valentina Puci, Allegra Rosa Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia Di Pinto, Franco Michelino Fiore, Armado Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massara, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Pietro Modugno, Maurizio Maiorano, Umberto Marcello Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni Maria Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaó, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio Stefano Tolva, Ilenia D'Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D'Arrigo, Giusi Basile, Dalmazio Frigerio, Gian Franco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio Massimo Oddi, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: chronic limb-threatening ischemia; outcome; sex; age; limb salvage

Online: 8 December 2022 (09:39:30 CET)

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Preprint REVIEW | doi:10.20944/preprints202201.0280.v1

Roles of Podoplanin in Malignant Progression of Tumor

Hiroyuki Suzuki, Mika K Kaneko, Yukinari Kato

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: podoplanin, PDPN, tumor malignancy, tumor marker, antibody therapy, cancer-specific monoclonal antibody, CasMab

Online: 19 January 2022 (16:05:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202207.0426.v1

Predicting the Cross-Coordinated Immune Response Dynamics in Sars-Cov-2 Infection: Implications for Disease Pathogenesis

Dmitry Grebennikov, Antonina Karsonova, Marina Loginova, Valentina Casella, Andreas Meyerhans, Gennady Bocharov

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: SARS-CoV-2 infection; innate immune response; antigen-specific immune response; kinetic coordination; mathematical model; pathogenesis, long COVID-19

Online: 27 July 2022 (15:11:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0185.v1

De Novo Assembly and Annotation of 11 Diverse Shrub Willow (Salix) Genomes Reveals Novel Gene Organization in Sex-Linked Regions

Brennan Hyden, Kai Feng, Timothy B Yates, Sara Jawdy, Chelsea Cereghino, Lawrence B Smart, Wellington Muchero

Subject: Biology And Life Sciences, Plant Sciences Keywords: Salix; shrub willow; genome assembly; sex determination

Online: 12 December 2022 (01:46:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202203.0286.v1

Classical-Relativistic Variability of Inertia of Photon Displacement Mass in the Matter and the Specific Vacuum Temperature

Daniel Souza Cardoso, José Rafael Bordin

Subject: Physical Sciences, Optics And Photonics Keywords: photon inertia; inertia momenta; photon inertia variability; photon inertia momenta variability; specific vacuum temperature; Abraham momenta; Minkowski momenta; relativistic energy wave; translational inertia; rotational inertia; photon displacement mass

Online: 21 March 2022 (11:21:56 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0167.v1

Preparation and Characterization of a Glycyrrhizic Acid-Based Drug Delivery System for Allergen-Specific Immunotherapy

Ekaterina Pashkina, Veronika Evseenko, Natalya Dumchenko, Maxim Zelikman, Alina Aktanova, Maria Bykova, Mikhail Khvostov, Aleksandr Dushkin, Vladimir Kozlov

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: drug delivery; allergen; glycyrrhizic acid; allergen-specific immunotherapy

Online: 10 December 2021 (08:50:57 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0436.v1

DNA Environment of Centromeres and Non-homologous Chromosomes Interactions in Mouse

Victor Spangenberg, Losev Michail, Volkhin Ilya, Svetlana Smirnova, Nikitin Pavel, Oxana Kolomiets

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Prophase I of meiosis; chromatin; synaptonemal complex; chromosome; satellite DNA; chromocenter; nuclear architecture; MSCI; sex chromosomes; interactions of non-homologous chromosomes; chromatin silencing

Online: 23 November 2021 (15:58:17 CET)

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Pericentromeric regions of chromosomes enriched in tandemly repeated satellite DNA although representing a significant part of eukaryotic genomes are still understudied mainly due to interdisciplinary knowledge gaps. Recent studies suggest their important role in genome regulation, karyotype stability and evolution. Thus, the idea of satellite DNA as a junk part of the genome was refuted. Integration of data about molecular composition, chromosome behaviour and details of in situ organization of pericentromeric regions is of great interest. The objective of this work was a cytogenetic analysis of the interactions of pericentromeric regions non-homologous chromosomes in mouse spermatocytes using immuno-FISH. We analysed two events: the associations between cerntomeric regions of X chromosome and autosomes, and associations between centromeric regions of autosomal bivalents forming chromocenters. We conclude that X chromosome form temporary synaptic associations with different autosomes in early meiotic prophase I which normally can be found at pachytene-diplotene without signs of pachytene arrest. These associations are formed between the satellite DNA-enriched centomeric regions of X chromosome and different autosomes but not involve the satellite-poor centromeric region of Y-chromosome. We suggest the mechanism of X chromosome competitive replacement from such associations during synaptic correction. We showed that centromeric region of the X chromosome remains free of γH2Ax-dependent chromatin inactivation, while Y chromosome is completely inactivated. This findings highlights the predominant role of associations between satellite DNA-enriched regions of different chromosomes including X. We assume that X-autosome temporary associations is a manifestation of an additional synaptic disorders checkpoint. These associations are normally corrected before the late diplotene. We revealed that the intense spreading conditions applied to the spermatocytes I nuclei did not lead to destruction of stretched chromatin fibers i.e. elongated chromocenters enriched in satellite DNA. Revealed by us tight associations between pericentromeric regions of different autosomal bivalents and X chromosome may represent the basis for repeat stability maintenance in autosomes an X chromosome. The consequences of our findings are discussed. We obtained the preparations of mouse spermatocytes nuclei in the meiotic prophase I using two approaches: standard and extremely intense surface spread techniques. Using immuno-FISH we visualized tandemly repeated mouse Major and Minor satellite DNA located in the pericentromeric regions of chromosomes and performed a morphological comparison of the standard- and intensely spreaded meiotic nuclei. Based on our results, we assume the remarkable strength of the chromocenter-mediated associations, “chromatin “bridges”, between different bivalents at the pachytene and diplotene stages. We have demonstrated that the chromocenter “bridges” between the centromeric ends of meiotic bivalents are enriched in both tandemly repeated Major and Minor satellite DNA. Association of centromeric regions of autosomal bivalents and X-chromosome but not with Y-chromosome correlates with the absence of Major and Minor satellites on Y-chromosome. We suggest that revealed tight associations between pericentromeric regions of bivalents may represent the network-like system providing dynamic stability of chromosomal territories, as well as add new data for the hypothesis of ectopic recombination in these regions which supports sequence homogeneity between non-homologous chromosomes and does not contradict the meiotic restrictions imposed by the crossing-over interference near centromeres. We conclude that nuclear architecture in meio-sis may play an essential role in contacts between the non-homologous chromosomes providing the specific characteristics of pericentromeric DNA.

Preprint REVIEW | doi:10.20944/preprints202306.0833.v1

The Price of Human Evolution: Cancer-Testis-Antigens, the Decline in Male Fertility and the Increase in Cancer

Jekaterina Erenpreisa, Ninel M Vainshelbaum, Marija Lazovska, Roberts Karklins, Kristine Salmina, Pawel Zayakin, Felikss Rumnieks, Inna Inashkina, Dace Pjanova, Juris Erenpreiss

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Cancer-testis-antigens; parthenogenetic; polyploid giant cancer cells; PGCCs; genome fragility; phylostratigraphic analysis; innate immunity-placentation; endogenous retroviruses; sex determination; male infertility; endocrine disruption; environmental pollution

Online: 12 June 2023 (12:59:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0116.v2

Frail Males on the American Frontier: The Role of Environmental Harshness on Sex Ratios at Birth Across a Period of Rapid Industrialization

Ryan Schacht, Mike Hollingshaus, Heidi Hanson, Tim Bruckner, Shane J Macfarlan, Douglas Tharp, Ken R Smith

Subject: Social Sciences, Gender And Sexuality Studies Keywords: sex ratio; prenatal stress; demography; ecological stress

Online: 6 April 2021 (14:57:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0452.v1

Small Cajal body-specific RNA12 Promotes Carcinogenesis through Modulating Extracellular Matrix Signaling in Bladder Cancer

Qinchen Lu, Yuting Tao, Jiandong Wang, Jialing Zhong, Zhao Zhang, Chao Feng, Xi Wang, Tianyu Li, Qiuyan Wang, Rongquan He, Yuanliang Xie

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: small Cajal body-specific RNA12; bladder cancer; ECM; transcription factor; H2AFZ

Online: 7 December 2023 (07:02:01 CET)

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Abstract Background: Small Cajal body-specific RNAs (scaRNAs) are a specific subset of small nucleolar RNAs (snoRNAs) that have recently been emerged as pivotal contributors in diverse physiological and pathological processes. However, their defined roles in carcinogenesis remain largely elusive. This study aims to explore the potential function and mechanism of SCARNA12 in bladder cancer (BLCA) and to provide a theoretical basis for further investigations into the biological functionalities of scaRNAs. Materials and Methods: TCGA, GEO and GTEx data sets were used to analyze the expression of SCARNA12 and its clinicopathological significance in BLCA. Quantitative real-time PCR (qPCR) and in situ hybridization were applied to validate the expression of SCARNA12 in both BLCA cell lines and tissues. RNA sequencing (RNA-seq) combined with bioinformatics analyses were conducted to reveal the changes of gene expression patterns and functional pathways in BLCA patients with different expression of SCARNA12 and T24 cell lines upon SCARNA12 knockdown. Single-cell mass cytometry (CyTOF) was then used to evaluate the tumor-related cell cluster affected by SCARNA12. Moreover, SCARNA12 was stably knocked down in T24 and UMUC3 cell lines by lentivirus-mediated CRISPR/Cas9 approach. The biological effects of SCARNA12 on the proliferation, clonogenic, migration, invasion, cell apoptosis, cell cycle and tumor growth were assessed by in vitro MTT, colony formation, wound healing, transwell, flow cytometry assays and in vivo nude mice xenograft models, respectively. Finally, chromatin isolation by RNA purification (ChIRP) experiment were further conducted to delineate the potential mechanisms of SCARNA12 in BLCA. Results: The expression of SCARNA12 was significantly up-regulated in both BLCA tissues and cell lines. RNA-seq data elucidated that SCARAN12 may play a potential role in cell adhesion and extracellular matrix (ECM) related signaling pathways. CyTOF results further showed that an ECM-related cell cluster with vimentin+, CD13+, CD44+, CD47+ was enriched in BLCA patients with high SCARNA12 expression. Additionally, SCARNA12 knockdown significantly inhibited the proliferation, colony formation, migration, and invasion abilities in T24 and UMUC3 cell lines. SCARNA12 knockdown prompted cell arrest in the G0/G1 and G2/M phase, and promoted the apoptosis in T24 and UMUC3 cell lines. Furthermore, SCARNA12 knockdown could suppress the in vivo tumor growth in nude mice. ChIRP experiment further suggested that SCARNA12 may combine transcription factors H2AFZ to modulate transcription program and then affect BLCA progression. Conclusion: Our study is the first to propose aberrant alteration of SCARNA12 and elucidate its potential oncogenic roles in BLCA via the modulation of ECM signaling. The interaction of SCARNA12 with the transcriptional factor H2AFZ emerges as a key contributor to the carcinogenesis and progression of BLCA. These findings suggest SCARNA12 may serve as a diagnostic biomarker and potential therapeutic target for the treatment of BLCA.

Preprint ARTICLE | doi:10.20944/preprints202103.0160.v1

99mTc-Sestamibi Bioaccumulation Can Induce the Apoptosis in Breast Cancer Cells: Molecular and Clinical Perspectives

Nicoletta Urbano, Manuel Scimeca, Rita Bonfiglio, Alessandro Mauriello, Elena Bonanno, Orazio Schillaci

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: 99mTc-Sestamibi; Breast Specific Gamma Imaging; Breast Cancer; Apoptosis; Theragnostic

Online: 4 March 2021 (14:00:05 CET)

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Preprint ARTICLE | doi:10.20944/preprints202205.0409.v1

Are There Sex Differences in Self-Reported Childhood Maltreatment in Major Depressive and Bipolar Disorders? A Retrospective Cross-Sectional Study

Daniela Caldirola, Tatiana Torti, Francesco Cuniberti, Silvia Daccò, Alessandra Alciati, Koen Schruers, Giovanni Martinotti, Domenico De Berardis, Giampaolo Perna

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: childhood trauma; major depressive disorder; bipolar disorder; sex difference; age

Online: 31 May 2022 (03:39:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1435.v1

A-DSCNN: Depthwise Separable Convolutional Neural Network Inference Chip Design using an Approximate Multiplier

Jin-Jia Shang, Nicholas Phipps, I-Chyn Wey, Tee Hui Teo

Subject: Engineering, Electrical And Electronic Engineering Keywords: Application-Specific Integrated Circuits; Approximate Multiplier; CMOS; Convolutional Neural Network; Depthwise Separable Convolution; Processing Element

Online: 21 June 2023 (03:49:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0605.v1

Synthesis and Evaluation of 99mTc-labeled PSMA-targeted Tracers Based on the Lys-urea-Aad Pharmacophore for Detecting Prostate Cancer with Single Photon Emission Computed Tomography

Kelly Lu, Chengcheng Zhang, Zhengxing Zhang, Hsiou-Ting Kuo, Nadine Colpo, François Bénard, Kuo-Shyan Lin

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: prostate-specific membrane antigen (PSMA); technetium-99m; single photon emission computed tomography (SPECT); molecular imaging; HYNIC

Online: 8 June 2023 (08:11:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0587.v1

Development of Clause Complexity in Children with Specific Language Impairment/Language Development Disorder. A Longitudinal Study

Claudia Araya, Carmen Julia Coloma, Camilo Quezada, Paula Benavente

Subject: Social Sciences, Language And Linguistics Keywords: Grammar; clause complexity; longitudinal study; Specific Language Impairment (SLI); Developmental Language Disorder (DLD).

Online: 8 June 2023 (04:41:09 CEST)

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Preprint REVIEW | doi:10.20944/preprints202302.0305.v1

National and Ethnic Mutation Frequency Databases: Cross Comparison and Future Direction

Shumaila Khan, Mehmood Alam, Shahnaz Khan, Wahab Khan, Ihsan Rabbi, Iqbal Qasim

Subject: Computer Science And Mathematics, Information Systems Keywords: genetic databases; mutation disorders; ethnic specific databases

Online: 17 February 2023 (07:59:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0377.v1

Calibrating A Comprehensive Immune Age Metric to Analyze the Cross Sectional Age-Related Decline in Cardiorespiratory Fitness

Peter Bröde, Maren Claus, Patrick D. Gajewski, Stephan Getzmann, Klaus Golka, Jan G. Hengstler, Edmund Wascher, Carsten Watzl

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: aging; immunosenescence; physical fitness; physical activity; obesity; sex

Online: 26 September 2022 (05:00:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0023.v1

PSI-mediated Vacuolar Sorting is not Disrupted in Arabidopsis leb-2 Mutant

Tatiana Cardoso, Miguel Sampaio, João Neves, Sofia Oliveira, Inês Moura, Ana Séneca, José Pissarra, Susana Pereira, Cláudia Pereira

Subject: Biology And Life Sciences, Plant Sciences Keywords: Arabidopsis; ER-bodies; aspartic proteinases; Plant Specific Insert; Vacuolar sorting; Endomembranes

Online: 3 April 2023 (10:36:29 CEST)

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Working Paper ARTICLE

Male Disadvantage in Oxidative Stress-Associated Complications of Prematurity: A Systematic Review, Meta-analysis and Meta-regression

Elke van Westering-Kroon, Maurice J Huizing, Eduardo Villamor-Martínez, Eduardo Villamor

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: preterm birth; oxidative stress; sex differences; male disadvantage; female advantage; bronchopulmonary dysplasia; retinopathy of prematurity; necrotizing enterocolitis; intraventricular hemorrhage; periventricular leukomalacia; mortality.

Online: 3 September 2021 (11:08:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1048.v1

How Structural Modifications of Insulin Icodec Contributes to Its Prolonged Duration of Action: A Structural and Biophysical Perspective

Wei Li

Subject: Biology And Life Sciences, Biophysics Keywords: Insulin icodec; Molecular design; Site-specific mutation; Structural biophysics

Online: 16 November 2023 (11:16:57 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.1142.v1

Editing Metabolism, Sex, and Microbiome: How can we Help Poplar Resist Pathogens?

Maxim A. Kovalev, Natalya S. Gladysh, Alina S. Bogdanova, Nadezhda L. Bolsheva, Mikhail I. Popchenko, Anna V. Kudryavtseva

Subject: Biology And Life Sciences, Plant Sciences Keywords: Populus; plant pathogens; plant immunity; plant microbiome; plant sex

Online: 15 December 2023 (08:50:47 CET)

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Preprint REVIEW | doi:10.20944/preprints202302.0516.v1

The Confound of Hemodynamic Response Function Variability in Resting-State Functional MRI Studies

D Rangaprakash, Robert Barry, Gopikrishna Deshpande

Subject: Biology And Life Sciences, Biophysics Keywords: BOLD fMRI; HRF; resting state connectivity; aging; sex differences; confound

Online: 28 February 2023 (09:32:04 CET)

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Preprint REVIEW | doi:10.20944/preprints202305.0677.v1

Gender Differences in Oxidative Stress in Relation to Cancer Susceptibility and Survival

Alessandro Allegra, Santino Caserta, Sara Genovese, Giovanni Pioggia, Sebastiano Gangemi

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: gender differences; cancer; oxidative stress; oestrogens; testosterone; antioxidant; reactive oxygen species; sex hormones; mitochondria.

Online: 10 May 2023 (03:44:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0457.v1

Lateral Flow Biosensor for microRNA Detection Based on Peptide Nucleic Acid Probes

Aodi Liu, Qingjie Xia, Yang Huang, Haoheng Liu, Jin Tian, Kezhou Li

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Peptide nucleic acid; microRNA detection; duplex specific nuclease; Early diagnosis of pancreatic cancer; Lateral flow biosensor

Online: 18 April 2023 (02:48:27 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1165.v1

Considerations, Advances, and Challenges Associated with the Use of Specific Emitter Identification in the Security of Internet of Things Deployments: A Survey

Joshua H. Tyler, Mohamed K.M. Fadul, Donald R. Reising

Subject: Engineering, Electrical And Electronic Engineering Keywords: Specific Emitter Identification, Radio Frequency Fingerprinting, Physical Layer Authentication, Physical Layer Security, Internet of Things

Online: 18 July 2023 (07:37:34 CEST)

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Initially introduced almost thirty years ago for the express purpose of providing electronic warfare systems the capabilities to detect, characterize, and identify radar emitters, Specific Emitter Identification (SEI) has recently received a lot of attention within the research community as a physical layer technique for securing Internet of Things (IoT) deployments. This attention is due in large part to SEI’s demonstrated success in passively and uniquely identifying wireless emitters using traditional machine learning and the success of Deep Learning (DL) within the natural language processing and computer vision areas. SEI exploits distinct and unintentional features present within an emitter’s transmitted signals. The existence of these distinctive and unintentional features is attributed to slight manufacturing and assembly variations that exist within and between the components, sub-systems, and systems that comprise an emitter’s Radio Frequency (RF) front end. Although sufficient to facilitate SEI, these features do not hinder normal operations such as detection, channel estimation, timing, and demodulation. However, despite the plethora of SEI publications, it has remained largely a focus of academic endeavors that primarily focus on proof-of-concept demonstration and little to no use in operational networks for various reasons. The focus of this survey is a review of SEI publications from the perspective of its use as a practical, effective, and usable IoT security mechanism, thus we use IoT requirements and constraints (e.g., wireless standard, nature of their deployment) as a lens through which each reviewed paper is analyzed. Previous surveys have not taken such an approach and have only used IoT as motivation, a setting, or a context. In this survey, we consider operating conditions, SEI threats, SEI at scale, publicly available data sets, and SEI considerations that are dictated by the fact that it is to be employed by IoT devices or IoT infrastructure.

Preprint ARTICLE | doi:10.20944/preprints202110.0288.v1

Sex Differences in the Associations of Nutrient Patterns with Total and Regional Adiposity: A Study of Middle-Aged Black South African Men and Women

Tshifhiwa Ratshikombo, Julia H. Goedecke, Melikhaya Soboyisi, Clement Kufe, Maphoko Masemola, Lisa K Micklesfield, Tinashe Chikowore

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Nutrient patterns; Obesity; Sex differences; Total and regional adiposity; South Africa

Online: 20 October 2021 (10:17:40 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202007.0073.v2

Sexual Dimorphism in the Multielemental Stoichiometric Phenotypes and Stoichiometric Niches of Spiders

Łukasz Sobczyk, Michał Filipiak, Marcin Czarnoleski

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: ecological stoichiometry; predator; spider; sex; nutrition; nutritional ecology; arthropod; nutrient cycling; trophic link; food web

Online: 29 July 2020 (09:40:28 CEST)

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Preprint REVIEW | doi:10.20944/preprints202207.0125.v1

A Fish of Multiple Faces, which Show us Enigmatic and Incredible Phenomena in Nature: Biology and Cytogenetics of the Genus Carassius

Martin Knytl, Adrian Forsythe, Lukáš Kalous

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: hybridization; sexuality; asexuality; biotype; species; sex determination; ploidy level; Carassius auratus complex

Online: 7 July 2022 (09:56:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0401.v1

Assessing Loss of Regulatory Divergence, Genome–Transcriptome Incongruence, and Preferential Expression Switching in Abaca/ Banana Backcrosses

Nelzo C. Ereful, Antonio G. Lalusin, Antonio C. Laurena

Subject: Biology And Life Sciences, Plant Sciences Keywords: abaca (Musa textilis); allelic imbalance; regulatory divergence; banana (M. balbisiana); allele-specific expression

Online: 29 June 2022 (09:34:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0134.v1

Autologous Cord Blood vs Individualized Supplements in Autistic Spectrum Disease – Results of the CORDUS Study

Felician Stancioiu, Radu Dumitrescu, Raluca Bogdan, Bogdan Ivanescu

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Autistic Spectrum Disease; ASD; Cord Blood; neuroinflammation; autologous; neuron specific enolase; NSE

Online: 3 January 2024 (08:47:28 CET)

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Preprint REVIEW | doi:10.20944/preprints202304.0989.v1

An Emerging Animal Model for Querying the Role of Whole Genome Duplication in Development, Evolution, and Disease

Mara Schvarzstein, Fatema Alam, Muhammad Toure, Judith L Yanowitz

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Whole genome duplication; tetraploidy; polyploidy; aneuploidy; Caenorhabditis; WGD model (List three to ten pertinent keywords specific to the article yet reasonably common within the subject discipline.)

Online: 26 April 2023 (12:43:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0031.v1

Mast Cells as a Potential Target of Molecular Hydrogen in Regulating the Local Tissue Microenvironment

Dmitri Atiakshin, Andrey Kostin, Artem Volodkin, Anna Nazarova, Viktoriya Shishkina, Dmitry Esaulenko, Igor Buchwalow, Markus Tiemann, Mami Noda

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: molecular hydrogen; reactive oxygen intermediates; mast cells; secretome; specific mast cell proteases; local tissue microenvironment; inflammation

Online: 1 May 2023 (11:54:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1276.v1

Understanding Transcription Factors and How They Affect Metabolic Processes in Cucumber Sex Determination

Szymon Turek, Aparna Aparna, Agnieszka Skarzyńska, Wojciech Pląder, Magdalena Pawełkowicz

Subject: Biology And Life Sciences, Plant Sciences Keywords: transcription factors; interactome network; sex development; sex determination; Cucumber (Cucumis sativus); metabolomic processes

Online: 1 May 2023 (00:01:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0761.v1

A Narrative Review on a Deep Understanding of the Dominant Physiological Energy Systems in Basketball and the Importance of Specificity and Uniqueness in Measuring Basketball Players

Asaf Shalom, Roni Gottlieb, Pedro E. Alcaraz, Julio Calleja-González

Subject: Biology And Life Sciences, Life Sciences Keywords: physiology; basketball; testing; explosive; unique; specific movements

Online: 12 October 2023 (05:32:49 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202305.0013.v1

Renal Mast Cell Specific Proteases in the Pathogenesis of Tubulointerstitial Fibrosis: Case Report

Dmitri Atiakshin, Sergey Morozov, Vladimir Dlin, Andrey Kostin, Michael Ignatyuk, Galina Kuzovleva, Sergey Baiko, Igor Buchwalow, Markus Tiemann

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: mast cells; tryptase; carboxypeptidase A3; chymase; fibrogenic niche; kidney fibrosis; specific tissue microenvironment

Online: 1 May 2023 (04:12:22 CEST)

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Kidney fibrosis is a complex polyetiological progressive disease manifesting as an intraorgan proliferation of the connective tissue with the appearance of cicatricial changes. However, pathogenetic mechanisms of renal fibrosis remain poorly understood. The paper highlights features of the protease phenotype and histotopography of mast cells (MCs) found in the kidney, as the key players in extracellular matrix remodeling in nephrofibrosis, detected in a patient diagnosed with "nephrosclerosis with the non-functioning upper pole of the duplex right kidney in the junction of refluxing megaureter of the upper pole of the duplex right kidney". The highest content of MCs was found in the kidney areas with fibrotic changes. MCs were actively involved in the development of inflammatory and fibrotic changes in limited loci of the specific tissue microenvironment of the kidney through targeted secretion of tryptase, chymase, and carboxypeptidase A3 to the vascular endothelium, nephron epithelium, interstitium cells, and components of the intercellular substance. The formation of a profibrotic environment in the kidney interstitium was facilitated by epigenetic effects of tryptase promoting epithelial-mesenchymal transition (EMT) of the nephron epithelium. A selectively increased number and targeted secretory activity of MCs in limited loci of the kidney parenchyma without visible pathological changes evidence a trigger pathogenetic role of specific proteases in the formation of a local microenvironment with a profibrogenic metabolic profile. Thus, MCs are actively involved in the mechanisms of formation and evolution of fibrogenic niches in the kidney; this fact can be used to develop new strategies for therapeutic options of tubulointerstitial fibrosis resulted from chronic kidney injury. Further study of the molecular phenotype of mast cells reveals new fundamental mechanisms of fibrosis pathogenesis being a significant tool for personalized therapy in nephrology.

Preprint ARTICLE | doi:10.20944/preprints202112.0075.v1

Dynamic Patterns of Sex Chromosome Evolution in Neognath Birds: Many Independent Barriers to Recombination at the ATP5F1A Locus

Rebecca T. Kimball, Edward L. Braun

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Sex chromosomes; Hill-Robertson interference; male-driven molecular evolution; pseudoautosomal region; pseudogenes; purifying selection; protein structure; Psittaciformes

Online: 6 December 2021 (13:21:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202104.0323.v1

Evaluation of Sex Differences in Murine Diabetic Ketoacidosis and Neutropenic Models of Invasive Mucormycosis

Teclegiorgis Gebremariam, Sondus Alkhazraji, Abdullah Alqarihi, Nathan P. Wiederhold, Laura K. Najvar, Thomas F. Patterson, Scott G. Filler, Ashraf S. S. Ibrahim

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Rhizopus; Mucor; Mucormycosis; Murine; sex

Online: 12 April 2021 (21:07:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0211.v1

Klotho Regulated by Estrogen Plays a Key Role in Sex Differences in Stress Resilience

Zhinei Tan, Yongxia Li, Yinzheng Guan, Javed Iqbal, Chenyue Wang, Xinming Ma

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: klotho; estrogen; hippocampus; chronic stress; sex difference; stress resilience

Online: 13 December 2022 (01:09:36 CET)

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Working Paper ARTICLE

Domain-Specific Stimulation of Executive Functioning in Low-Preforming Students with a Roma Background: Cognitive Potential Of Mathematics

Iveta Kovalčíková, Jochanan Veerbeek, Bart Vogelaar, Alena Prídavková, Ján Ferjenčík, Edita Šimčíková, Blanka Tomková

Subject: Business, Economics And Management, Accounting And Taxation Keywords: executive functioning; domain-specific cognitive stimulation; math; low-performing student; Roma ethnic group.

Online: 13 May 2021 (13:06:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0810.v1

Risk Factors and Prognostic Nomogram for Early-onset Endometrial Cancer: A SEER-Based Analysis

Yunfeng Zheng, Peng Jiang, Qingyu Shen, Yuan Tu, Yuzhen Huang, Jinyu Wang, Chenfan Tian, Shikai Gou, Rui Yuan

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: early-onset endometrial cancer; prognostic nomogram; overall survival; cancer-specific survival; SEER

Online: 13 October 2023 (03:02:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0237.v1

Incidence and Risk Factors for Cerebrovascular-Specific Mortality in Patients with Colorectal Cancer: A Registry-Based Cohort Study Involving 563298 Patients

Wei Huang, Ming Tang, Yun-Liang Chen, Tao-Lan Zhang, Tao Hong, Jing Li, Guo-Hua Lv, Yiguo Yan, Zhi-Hua Ouyang, Mingxiang Zou

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Colorectal cancer; Cerebrovascular-specific mortality; Cerebrovascular-specific diseases; Incidence; Risk factors

Online: 12 November 2021 (16:47:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202303.0049.v1

Dietary and Sexual Correlates of Gut Microbiota in the Japa-Nese Gecko, Gekko japonicus (Schlegel, 1836)

Xin-Ru Jiang, Ying-Yu Dai, Yu-Rong Wang, Kun Guo, Yu Du, Jian-Fang Gao, Long-Hui Lin, Peng Li, Hong Li, Xiang Ji, Yan-Fu Qu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: ASVs; diet habit; Gekko japonicus; gut microbiota; sex

Online: 3 March 2023 (01:31:32 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.0130.v1

Sex Differences in Visceral Pain and Comorbidities: Clinical Outcomes, Preclinical Models, and Cellular and Molecular Mechanisms

Namrata Tiwari, Liya Y Qiao

Subject: Medicine And Pharmacology, Anesthesiology And Pain Medicine Keywords: pain; visceral; sex; clinical; preclinical; molecule

Online: 3 January 2024 (07:41:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1324.v1

Questionnaire of Physiotherapeutic Specific Exercises of Scoliosis – QPSSE

Theodoros B. Grivas, Dimitra Dadakaridou, Stavroula A. Fokidi, Alexandros Kastrinis, Melpomeni Kosti, Constantinos Michas, Marianna Oikonomaki, Evangelos Theodosopoulos, Artemis Margarita Griva

Subject: Public Health And Healthcare, Physical Therapy, Sports Therapy And Rehabilitation Keywords: adolescent idiopathic scoliosis; physiotherapeutic specific exercises; questionnaire; quality of life

Online: 21 November 2023 (13:43:07 CET)

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Study design: Creating and psychometric testing of a new QoL Questionnaire about Physiotherapeutic Specific Exercises of Scoliosis (Questionnaire of Physiotherapeutic Specific Exercises of Scoliosis -QPSSE). Purpose: The purpose of this study is to create a reliable and valid questionnaire for patients suffering mild and moderate adolescent idiopathic scoliosis (AIS) who have been treated with Physiotherapeutic Specific Exercises of Scoliosis (PSSE) in order to evaluate their quality of life. Materials and Methods: The developed questionnaire was based on a thorough literature review as well as on authors experience. It consists of 53 questions, of which 37 have a positive meaning, 15 have a negative meaning, and one is multiple choice question, additionally there are six “open” questions. Except for the multiple choice question, all other questions are answered on a Likert scale ranging from 1 to 5 scores. 5 represents a positive meaning or very much, whereas 1 stands for a negative meaning or not at all. Questions were developed by the authors who subsequently categorized the 53 questions into the following 8 domains: physical functioning, self-image, Physiotherapeutic Scoliosis-Specific Exercises (PSSEs), psychosocial functioning, cognitive functioning, compliance, motivation and pain. A pilot study was conducted so that we could calculate Cronbach’s Alpha based on the outcome. Due to the COVID-19 pandemic, the authors worked through the Zoom online platform to structure the questionnaire. Results: Pearson's correlation coefficient was used for all correlations evaluated. P values of less than 0.05 were considered to be significant. Internal consistency was evaluated with Cronbach's alpha. Although there were very few missing values, accounting for 0.78% of the total values of the questionnaire, expectation maximization likelihood algorithm was used to impute data. IBM® SPSS® Statistics Software v.25 was used for the analysis. Cronbach's alpha coefficients for the overall score was 0.84. Conclusion: This original QPSSE was found to be a reliable and valid tool for AIS treated conservatively with PSSE and for their clinicians.

Preprint ARTICLE | doi:10.20944/preprints202301.0273.v1

Identifying Patients in Whom the Follow-up Scheme after Robot-assisted Radical Prostatectomy Could be Optimized in the First Year after Surgery: Reducing Healthcare Burden

Hans Veerman, Sophia Van der Graaf, Dennie Meijer, Marinus Jan Hagens, Corinne N. Tillier, Pim J. Van Leeuwen, Henk G. Van der Poel, André N. Vis

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: prostatic neoplasms; prostatectomy; prostate-specific antigen; follow-up; aftercare; recurrence; delivery of healthcare

Online: 16 January 2023 (07:38:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0361.v1

Impact of Sex in Left Atrial Indices for Prognosis of Heart Failure with Preserved Ejection Fraction

Shiro Hoshida, Koichi Tachibana, Nobutaka Masunaga, Yukinori Shinoda, Tomoko Minamisaka, Hirooki Inui, Keisuke Ueno, Masahiro Seo, Masamichi Yano, Takaharu Hayashi, Akito Nakagawa, Yusuke Nakagawa, Shunsuke Tamaki, Takahisa Yamada, Yoshio Yasumura, Yohei Sotomi, Shungo Hikoso, Daisaku Nakatani, Yasushi Sakata

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Ed/Ea; Fine-Gray model; HFpEF; LAVI; sex

Online: 23 September 2022 (08:09:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202211.0355.v1

Risk Assessment of Transgender People: Development of Rodent Models Mimicking Gender-Affirming Hormone Therapies and Identification of Sex-Dimorphic Liver Genes as Novel Biomarkers of Sex Transition

Roberta Tassinari, Alessia Tammaro, Gabriele Lori, Sabrina Tait, Andrea Martinelli, Luigia Cangemi, Paolo Frassanito, Francesca Maranghi

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: testosterone; estrogen; cyprotenone acetate; masculinizing; feminizing; cytochrome P450; sex-specific genes

Online: 18 November 2022 (10:10:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.2078.v1

The Role of Strength-Related Factors on Psychological Readiness for Return to Sport following Anterior Cruciate Ligament (ACL) Reconstruction

Oliver Lee, Mark A Williams, Clare D Shaw, Anne Delextrat

Subject: Public Health And Healthcare, Physical Therapy, Sports Therapy And Rehabilitation Keywords: angle-specific torque; rate of force development; time since surgery; rehabilitation

Online: 30 August 2023 (11:19:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1834.v2

Global Eggshell Properties: Characterizing Variability for Sustainable Partial Cement Replacement in Hong Kong’s Concrete

Sohail Maqsood, Lam Siu Shu Eddie, Hei Yi Ng, Muhammad Afzal, Safi Ur Rehman, Muhammad Faisal Ayyub

Subject: Engineering, Civil Engineering Keywords: Calcined Eggshells; Calcium Carbonate; Compressive Strength; Eggshells; Free Calcium Oxide; Specific Gravity; Thermogravimetric Analysis

Online: 27 December 2023 (05:41:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0711.v1

Young Women’s Attitudes and Concerns on Pornography and Their Sexual Experiences: A Qualitative Approach

Mónica Fernández-Ruiz, Olga María López-Entrambasaguas, Jose Manuel Martínez-Linares, José Granero-Molina

Subject: Public Health And Healthcare, Primary Health Care Keywords: pornography; qualitative research; sexual behavior; sex education; sexual health; young women

Online: 9 August 2023 (09:05:39 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.0845.v1

The Genetic Basis Underpinning Sexually Selected Traits across Different Animal Lineages: Are the Genetic Mechanisms in Common?

Ji Hyoun Kang

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: co-option; evolutionary novelty; male ornament; male-specific trait; sexual selection

Online: 10 January 2024 (14:43:33 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.0496.v1

Cell Type Specific Extracellular Vesicles and Their Impact on Health and Disease

Sohil Amin, Hamed Massoumi, Deepshikha Tewari, Arnab Karmaker Roy, Madhurima Chaudhuri, Cedra Jazayerli, Abhi Krishan, Mannat Singh, Mohammad Soleimani, Esra Karaca, Arash Mirzaei, Victor H Guaiquil, Mark I Rosenblatt, Ali R Djalilian, Elmira Jalilian

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: cell type specific extracellular vesicles; extracellular vesicles; exosomes; intercellular communication; diagnostic biomarkers; disease pathogensis

Online: 8 February 2024 (09:18:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202303.0165.v1

Modelling Time–Dependent Flow through Railway Ballast

Raed Alrdadi, Michael H. Meylan

Subject: Engineering, Civil Engineering Keywords: free surface; steady state; unsteady state; railway ballast; fouling ratio; specific yield; finite element method

Online: 9 March 2023 (03:31:03 CET)

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Preprint REVIEW | doi:10.20944/preprints202303.0371.v1

Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing

Tamer Butto, Kanak Mungikar, Peter Baumann, Jennifer Winter, Beat Lutz, Susanne Gerber

Subject: Medicine And Pharmacology, Hematology Keywords: Nuclei isolation; Next-generation sequencing; Cell-type specific isolation; Epigenetics; Transcriptomics; Single cell sequencing; Single nucleus sequencing

Online: 21 March 2023 (06:40:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.1870.v1

Exploring the Effects of Rearing Densities on Epigenetic Modifications in the Zebrafish Gonads

Alejandro Valdivieso, Marta Caballero-Huertas, Javier Moraleda-Prados, Francesc Piferrer, Laia Ribas

Subject: Biology And Life Sciences, Aquatic Science Keywords: stress; gonad; sex; methylation; masculinization; rearing

Online: 27 September 2023 (10:46:16 CEST)

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Working Paper ARTICLE

Comparison of BSGI and MRI as Approaches to Evaluating Residual Tumor Status After Neoadjuvant Chemotherapy in Chinese Women With Breast Cancer

Hongbiao Liu, Hongwei Zhan, Ying Zhang, Gangqiang He, Hui Wang, Qiaoxia Zhang, Lili Zheng

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: breast-specific gamma imaging (BSGI); neoadjuvant chemotherapy (NAC); breast cancer; Magnetic resonance imaging (MRI); residual tumor size

Online: 6 July 2021 (12:40:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1267.v1

A Female-Specific Treatment Group for ADHD – Description of the Programme and Qualitative Analysis of First Experiences

Maxime de Jong, Dora S.R.M. Wynchank, Marieke Michielsen, Aartjan T.F. Beekman, Sandra J.J. Kooij

Subject: Medicine And Pharmacology, Psychiatry And Mental Health Keywords: attention deficit/hyperactivity disorder (ADHD); women; menstrual cycle; non-pharmacological treatment; group treatment; sex hormones; premenstrual; female specific therapy; self-awareness; self-acceptance

Online: 21 March 2024 (11:26:38 CET)

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Preprint REVIEW | doi:10.20944/preprints202304.1102.v1

Sex Specificities of Human Placental Adaptive Changes: The Influence of Maternal Obesity

Esther Dos Santos, Marta Hita Hernandez, Valérie Sérazin, François Vialard, Marie-Noëlle Dieudonné

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: Maternal obesity; placenta; environmental adaptation; sex specificities; reproduction

Online: 28 April 2023 (04:06:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0262.v1

Synthesis, ¹²³I-Radiolabeling Optimization, and Initial Preclinical Evaluation of Novel Urea-Based PSMA Inhibitors with Tributylstannyl Prosthetic Group in Their Structures

Lutfi A. Hasnowo, Maria S. Larkina, Evgenii V. Plotnikov, Vitalina Bodenko, Feruza Yuldasheva, Elena Stasyuk, Stanislav A. Petrov, Nikolai Y. Zyk, Aleksei E. Machulkin, Nikolay I. Vorozhtsov, Elena K. Beloglazkina, Valentine G. Nenajdenko, Vladimir Tolmachev, Anna Orlova, Alexander G. Majouga, Mekhman S. Yusubov

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: DCL ligand; iodine radioisotopes; radiolabeled pharmaceuticals; targeted delivery; prostatic specific membrane antigen; prostate cancer

Online: 5 July 2023 (04:43:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0646.v1

Pacing Strategy of 800-Meter and 1500-Meter Freestyle Swimming Finals in the World Championships According to the Performance in Males and Females of Different Age Groups

Sabrina Demarie, Jean Renaud Pycke, Alessia Pizzuti, Veronique Billat

Subject: Biology And Life Sciences, Other Keywords: energy cost; endurance; training; technique; tactic; fatigue; performance; time series; mathematical modelling; sex difference

Online: 11 September 2023 (14:45:18 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202211.0215.v1

Alterations in Kidney Structures Caused by Age Vary According to Sex and Dehydration Condition

Susana Quirós Cognuck, Lucas Ferreira de Almeida, Wagner L. Reis, Márcia S. Silva, Gislaine Almeida Pereira, Sandra V. Zorro, André S. Mecawi, Terezila Machado Coimbra, Lucila L.K. Elias, José Antunes Antunes Rodrigues

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: aging; body composition; kidney; sex differences

Online: 11 November 2022 (03:53:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0053.v1

Evaluation of Equine Fecal Egg Count Tests for Accurate Monitoring of Anthelmintic Resistance

Manigandan Lejeune, Sabine Mann, Holly White, Danielle Maguire, Jaime Hazard, Rebecca Young, Charles Stone, Doug Antczak, Dwight Bowman

Subject: Biology And Life Sciences, Parasitology Keywords: Equine strongyle; Fecal Egg Count; FEC; modified McMaster; Mini-FLOTAC; Wisconsin floatation; sugar 1.33 specific gravity; NaNO3 1.33 specific gravity; NaCl 1.20 specific gravity; ZnSO4 1.18 specific gravity; AAEP; anthelmintic resistance; FEC gold standard

Online: 1 August 2023 (10:32:58 CEST)

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Parasite control strategies such as blanket anthelmintic treatment of all horses in a herd or interval treatment regimen based on egg reappearance remain widely followed practices for the last several decades. These practices have resulted in the fast emergence of resistance in equine strongyles to all major classes of drugs on the market. In this regard, the guidelines set by the American Association of Equine Practitioners advocate evidence-based strongyle control in horses. It recommends targeted treatment of all heavy egg shedders [> 500 eggs per gram (EPG) of feces] while the low shedders (0-200 EPG) are left untreated to maintain susceptible parasite refugia in a herd. As 50-75% of adult horses in a herd are low shedders, preventing them from unnecessary anthelmintic exposure is critical to tackling resistance. There are various fecal egg count (FEC) techniques with many modifications and variations in use, but none is identified as a gold standard and methods comparison studies are lacking. We hypothesized that the diagnostic performance of commonly used quantitation methodologies differs and the FEC methods must be evaluated to determine the gold standard. In this regard, we performed methods comparison studies using polystyrene beads with size and specific gravity comparable to strongyle eggs, as proxy. The linearity of 12 commonly used FEC methodologies (3 techniques with 4 different solutions) was studied using bead standards in the clinically applicable range (63 to 1,000) to determine their suitability for strongyle egg counts. Deming regression analysis identified Mini-FLOTAC with sugar (specific gravity 1.33) as the test with the greatest coefficient of determination. All tests underestimated the true bead count, and we determined a correction factor (CF) for each test to estimate the true count. CF negates the preference for a gold standard test. Finally, we analyzed the validity of CF for 5 tests with R2>0.95 to accurately quantify strongyle eggs from 40 different horses. Overall, this study identified methodologies with the highest diagnostic performance and the limitations to standardizing routine FEC tests to promote uniformity in implementing AAEP parasite control guidelines.

Preprint ARTICLE | doi:10.20944/preprints202306.1817.v1

Sex/gender Differences in the Language Profiles of Italian Children with Autism Spectrum Disorder

Jessica Barsotti, Gloria Mangani, Roberta Nencioli, Antonio Narzisi, Lucia Pfanner, Anna Maria Chilosi, Paola Cipriani, Alice Mancini, Angela Cosenza, Raffaella Tancredi, Sara Calderoni

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: Language and communication; sex/gender; Autism Spectrum Disorder; children

Online: 26 June 2023 (13:54:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0468.v1

Pharmacokinetics of Four Tyrosine Kinase Inhibitors in Adult and Paediatric Chronic Myeloid Leukaemia Patients

Sarah Allegra, Emma Dondi, Francesco Chiara, Silvia De Francia

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: dasatinib; imatinib; nilotibin; ponatinib; children; therapeutic drug monitoring; sex and gender pharmacology; personalized medicine.

Online: 7 August 2023 (11:53:12 CEST)

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