Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

99mTc-Sestamibi Bioaccumulation Can Induce the Apoptosis in Breast Cancer Cells: Molecular and Clinical Perspectives

Version 1 : Received: 3 March 2021 / Approved: 4 March 2021 / Online: 4 March 2021 (14:00:05 CET)

A peer-reviewed article of this Preprint also exists.

Urbano, N.; Scimeca, M.; Bonfiglio, R.; Mauriello, A.; Bonanno, E.; Schillaci, O. [99mTc]Tc-Sestamibi Bioaccumulation Can Induce Apoptosis in Breast Cancer Cells: Molecular and Clinical Perspectives. Appl. Sci. 2021, 11, 2733. Urbano, N.; Scimeca, M.; Bonfiglio, R.; Mauriello, A.; Bonanno, E.; Schillaci, O. [99mTc]Tc-Sestamibi Bioaccumulation Can Induce Apoptosis in Breast Cancer Cells: Molecular and Clinical Perspectives. Appl. Sci. 2021, 11, 2733.

Journal reference: Appl. Sci. 2021, 11, 2733
DOI: 10.3390/app11062733

Abstract

The aim of this study was to investigate the possible role of 99mTc-sestamibi in the regulation of cancer cell proliferation and apoptosis. To this end, Te in vivo values of 99mTc-sestamibi uptake have been associated to the in-situ expression of both Ki67 and caspase-3. For in vitro investiga-tions BT-474 cells were incubated with three different concentration of 99mTc-sestamibi: 10µg/ml –1µg/ml – 0,1µg/ml. Expression of caspase-3 and Ki67, as well as the ultrastructure of cancer cells, were evaluated at T0 and after 24, 48, 72 and 120 hours after 99mTc-sestamibi incubation. Ex vivo data strengthened the known association between the sestamibi uptake and the Ki67 expression. Linear regression analysis showed a significant association between the sestamibi uptake and the number of apoptotic cells evaluated as caspase-3 positive breast cancer cells. As concern the in vitro data, a significant decrease of the proliferation index was observed in breast cancer cells incubated with high concentration of 99mTc-sestamibi (10µg/ml). Amazingly, a significant increase in caspase-3 positive cells in cultures incubated with 10µg/ml 99mTc-sestamibi was observed. This study suggested the possible role of sestamibi in the regulation of pathophysiological process involved in breast cancer.

Keywords

99mTc-Sestamibi; Breast Specific Gamma Imaging; Breast Cancer; Apoptosis; Theragnostic

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