Butto, T.; Mungikar, K.; Baumann, P.; Winter, J.; Lutz, B.; Gerber, S. Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing. Cells2023, 12, 1051.
Butto, T.; Mungikar, K.; Baumann, P.; Winter, J.; Lutz, B.; Gerber, S. Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing. Cells 2023, 12, 1051.
Butto, T.; Mungikar, K.; Baumann, P.; Winter, J.; Lutz, B.; Gerber, S. Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing. Cells2023, 12, 1051.
Butto, T.; Mungikar, K.; Baumann, P.; Winter, J.; Lutz, B.; Gerber, S. Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing. Cells 2023, 12, 1051.
Abstract
In the last decade, we have witnessed an upsurge in nuclei-based studies, particularly coupled with next-generation sequencing. Such studies aim at understanding the molecular states that exist in heterogeneous cell populations by applying increasingly more affordable sequencing approaches, in addition to optimized methodologies developed to isolate and select nuclei. Although these powerful new methods promise unprecedented insights, it is important to understand and critically consider the associated challenges. Here, we provide a comprehensive overview of the rise of nuclei-based studies and elaborate on their advantages and disadvantages. Improved designs and appropriate use of the various experimental strategies will result in acquiring biologically accurate and meaningful information.
Keywords
Nuclei isolation; Next-generation sequencing; Cell-type specific isolation; Epigenetics; Transcriptomics; Single cell sequencing; Single nucleus sequencing
Subject
Medicine and Pharmacology, Hematology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
