Liu, A.; Xia, Q.; Huang, Y.; Liu, H.; Tian, J.; Li, K. Lateral Flow Biosensor for microRNA Detection Based on Peptide Nucleic Acid Probes. Preprints2023, 2023040457. https://doi.org/10.20944/preprints202304.0457.v1
APA Style
Liu, A., Xia, Q., Huang, Y., Liu, H., Tian, J., & Li, K. (2023). Lateral Flow Biosensor for microRNA Detection Based on Peptide Nucleic Acid Probes. Preprints. https://doi.org/10.20944/preprints202304.0457.v1
Chicago/Turabian Style
Liu, A., Jin Tian and Kezhou Li. 2023 "Lateral Flow Biosensor for microRNA Detection Based on Peptide Nucleic Acid Probes" Preprints. https://doi.org/10.20944/preprints202304.0457.v1
Abstract
Malignancy is a fatal disease with an increasing incidence with each passing year, and the early diagnosis of malignancy can significantly improve the cure rate of patients, which has profound implications for saving patients' lives. The changes of miRNA are closely related to the occurrence of malignant tumors. The expression of miRNA in many malignant tumors is obviously different and unique from that in normal tissues. In this study, a strategy for amplifying microRNA (miRNA) signal was designed using duplex specific nuclease (DSN), which was then combined with Peptide nucleic acid (PNA) probe hybridization technology to prepare a sensitive and efficient paper-based lateral flow biosensor (LFB) for the detection of pancreatic cancer (PC) associated microRNA-10b. The performance evaluation results of the LFB showed that the detection limit of miR-NA-10b is 5 nM/mL, and carries demonstrated well specificity, stability, reproducibility. The biosensor prepared in this paper can provide accurate and rapid detection of miRNA-10b. The detection results play a guiding role in the early diagnosis of PC. Meeting the clinical need for largescale screening of individuals at high risk of PC. Providing new avenues to achieve early mass screening in individuals at high risk of PC.
Keywords
Peptide nucleic acid; microRNA detection; duplex specific nuclease; Early diagnosis of pancreatic cancer; Lateral flow biosensor
Subject
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
