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Preprint ARTICLE | doi:10.20944/preprints202404.0811.v1

Whole-Genome Deep Sequencing of the Healthy Adult Nasal Microbiome

Mark Cannon, Gustavo Ferrer, Mari Tesch, Matthew Schipma

Subject: Medicine And Pharmacology, Otolaryngology Keywords: Deep sequencing; nasal microbiome; gateway microbiome; diversity; richness; probiotics; nasal spray

Online: 11 April 2024 (11:27:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0220.v1

Lily Database: A Comprehensive Genomic Resource for the Liliaceae Family

Manosh Kumar Biswas, Sathish kumar Natarajan, Dhiman Biswas, Jewel Howlader, Jong-In Park, Ill-Sup Nou

Subject: Biology And Life Sciences, Horticulture Keywords: Genetic diversity; Germplasm; molecular markers; transcription factors; DEGs; genes.

Online: 4 October 2023 (09:03:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202010.0604.v1

People-Centeredness, Chronic Conditions and Diversity Sensitive eHealth: Exploring Emancipation of the ‘Health Care System’ and the ‘Patient’ in Health Informatics

Lars Botin, Pernille S. Bertelsen, Christian Nøhr

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: eHealth; end-user; chronic disease; participatory design; socio-technical; diversity in care

Online: 29 October 2020 (09:30:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0875.v1

Intestinal Bacteria Community Structures of Ruditapes philippinarum and Their Correlation with Environmental Factors

Chenyang Zhao, Xinyan Zhao, Jiaxi Li, Yingxu Chen, Shang Liu, Lei Fang, Zhongming Huo

Subject: Environmental And Earth Sciences, Environmental Science Keywords: Ruditapes philippinarum; environmental factors; diversity analysis；microbial community structure analysis；redundancy analysis

Online: 15 March 2024 (15:00:11 CET)

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The intestinal microbial community of aquatic animals is closely related to the environment and thus affects the health of aquatic animals. Here we investigated annual variation of intestinal bacteria community structures of Ruditapes philippinarum cultured using two different methods, bottom sown and raft hanging. Bacterial diversity of seawater and sediment in different seasons was examined as well. Correlation between R. philippinarum intestinal bacteria community structures and environmental factors was analyzed. The results showed that in the two aquculture areas where R. philippinarum was differentially cultured, bacteria community structure of seawater and sediment both altered with seasons. However, neither seasonal changes nor difference in culture methods could significantly affect the intestinal bacteria community structures of R. philippinarum. Additionally, in terms of the alpha diversity of intestinal bacteria communities, there was no significant difference in R. philippinarum cultured using the two methods. For benthic cultured clams, alpha diversity of the intestinal bacterial communities was often higher than that of the environmental bacterial communities. For raft hanging cultured calms, Alpha diversity of the intestinal bacterial communities was normally comparable with that of the environmental counterparts. Beta diversity analysis showed that the intestinal bacterial community structure of R. philippinarum was mainly determined by both deterministic and stochastic processes. Correlation analysis showed that the intestinal bacterial community structures of R. philippinarum were determined by a variety of environmental factors. For clams collected from the benthic culture area, the intestinal bacterial structure was more correlated with the physicochemical properties of sediment in comparing with those of seawater. For clams collected from the raft-culture area, the intestinal bacterial structure was mainly determined by the phsicochemical characteristics of seawater. This study helps us to better understand the bacterial community structure in complex aquaculture ecosystems, providing a reference for healthy shellfish aquaculture and ecosystem protection in aquaculture area.

Preprint REVIEW | doi:10.20944/preprints202301.0257.v2

Quantifying Transcriptome Diversity: A Review

Emma Jones, Anisha Haldar, Vishal Oza, Brittany Lasseigne

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Gene expression; transcriptome diversity; transcriptional variation; transcript diversity; isoform-level diversity; gene-level diversity

Online: 13 April 2023 (03:07:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1112.v1

Prevalence of Porcine Parvoviruses 1 to 6 and Porcine Circovirus 3 Infections and of Their Co-infections With Porcine Circovirus 2 in the Republic of Korea

In-Ohk Ouh, Ju-Yeon Lee, Hyeonhae Choi, Seo Young Moon, Jae Young Song, Bang-Hun Hyun, Dongmi Kwak, Yoon-Hee Lee, Choi-Kyu Park

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Porcine parvovirus 1-6; Porcine circovirus 2-3; Prevalence; Genetic diversity

Online: 16 May 2023 (06:02:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0404.v1

Effects of Land Cover on Taxonomic and Functional Diversity of the Bird Communities in a Subtropical Mountain

Wenwen Zhang, Shengjun Zhao, Xiao Yang, Jing Tian, Ding Chen, Yuan Yu, Jie Shi, Peng Cui, Xue Wang, Chunlin Li

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Bird community; Functional diversity; Taxonomic diversity; Landscape pattern; Mountain city

Online: 4 January 2024 (11:28:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202103.0254.v1

Distribution of Insect in Protected Forest Area in Ambon City

fransina latumahina

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Insects; Diversity; ecosystem health; Ambon

Online: 9 March 2021 (10:03:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints201912.0010.v1

Biological Hotspot of Ichthyoplankton in the Estuarine Environment Timbulsoko Village, Demak

Hanityo Adi Nugroho, Sutrisno Anggoro, Ita Widowati

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Diversity; Ecology; Fishes; Geoscience

Online: 2 December 2019 (10:38:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202206.0268.v1

Catch Composition, Seasonality and Biological Aspects of Sharks Caught in the Ecuadorian Pacific

Jesús Briones-Mendoza, Diego Mejía, Pol Carrasco-Puig

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: diversity; abundance; cartilaginous fish

Online: 20 June 2022 (10:10:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0050.v1

Diversity and Reassortment Rate of Influenza a Viruses in Wild Ducks and Gulls

Yulia Postnikova, Anastasia Treshchalina, Elizaveta Boravleva, Alexandra Gambaryan, Aydar Ishmukhametov, Mikhail Matrosovich, Ron A.M. Fouchier, Galina Sadykova, Alexei Prilipov, Natalia Lomakina

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Avian influenza; Reassortment; Diversity

Online: 5 May 2021 (12:32:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1730.v1

Genetic Diversity of Human Norovirus in Children Less Than 5 Years of Age With Acute Gastroenteritis in Rural Communities in the Gambia

Sheriffo Jagne, M. Jahangir Hossain, Martin Antoni, Abdul Karim Sesay, Muhammed Khalie Abdul, Nuredin I. Mohammed, Madikay Senghore, Alansana Darboe, Jarra Manneh, Abdoulie Kanteh, Dam Khan, Saikou Y Bah, Ndaw Sylvanus Peter, Osbourne Quaye, George Enyimah Armah

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Norovirus genetic diversity genogroups genotypes

Online: 24 January 2024 (10:15:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202001.0038.v1

Responses of Individual Plant Species, Functional Groups, α- and β-Diversity to Short-Term Grazing Exclusion under Severe Drought Episode in Long-Term Grazed Alfa-Steppes

Yacine Kouba, Saifi Merdes, Badreddine Saadali, Haroun Chenchouni

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Mediterranean steppes; drylands; alpha diversity; beta diversity; rangeland grazing; indicator species

Online: 5 January 2020 (15:22:33 CET)

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Grazing exclusion has been proved to be one of the main measures for rehabilitating degraded arid steppes. However, the effect of this management practice on plant species diversity and composition is ambiguous, specially under prolonged droughts. Concurrently considering the responses of individual plant species, diversity of functional groups, α-diversity, and β-diversity (and its components) may be crucial to the holistic understanding of grazing exclusion effects on plant communities under drought conditions. Here, we investigated the response of these diversity measures to short-term sheep exclusion under severe drought episode in arid steppes of Alfa-grass (Stipa tenacissima) with a long evolutionary history of livestock grazing. Individual species responses were tested based on species occurrence and abundance in either grazed or grazing-excluded steppes, in addition, we used indicator species analysis to assess the strength of the association between plant species and management type. Likewise, α-diversity, abundance- and incidence-based β-diversity, as well as the functional groups’ diversities were quantified using Hill Numbers and compared between the two management types. Sheep grazing exclusion enabled the recovery of various Alfa-steppe indicator species and improved the size of regional species pool, overall α-diversity, and the diversity of therophytes. This management practice decreased the abundance-based β-diversity and the nestedness-resultant fraction of the incidence-based β-diversity at the local scale, while at the landscape scale increased the abundance-based β-diversity and its balanced variation fraction and reduced the incidence-based β-diversity and its turnover component. Furthermore, protection from grazing altered β-diversities scaling patterns by maintaining higher balanced variation in species abundance at large spatial scale and greater abundance-gradient in species composition at the fine-scale. Our results suggest that the implementation of short-term grazing exclusion in degraded arid steppes would be the appropriate management practice for vegetation restoration and plant diversity conservation during prolonged drought periods.

Preprint ARTICLE | doi:10.20944/preprints202402.0103.v1

Microsatellite Sequence Polymorphisms Reveals Substantial Diversity in Caribbean Breadfruit [Artocarpus altilis (Parkinson) Fosberg] Germplasm

Oral O Daley, Angela T Alleyne, Laura B Roberts-Nkrumah, Lambert A Motilal

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Crop germplasm; genetic diversity; nucleotide diversity; nucleotide polymorphism; SSR markers; un-derutilized crop

Online: 2 February 2024 (04:54:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0056.v2

Women’s Participation in Leadership Roles in a Single Canadian Paramedic Service

Justin Mausz, Sarah Salvis, Dominique Arseneau-Bruneau, Elizabeth Donnelly

Subject: Social Sciences, Sociology Keywords: Emergency Medical Services; paramedics; gender; diversity

Online: 15 February 2023 (04:34:52 CET)

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Introduction: Like other public safety professions, paramedicine has historically been a male-dominated occupation, both in the demography of its workforce and in its organizational culture. Although women are increasingly choosing paramedicine as a career, participation in leadership roles remains limited. Drawing on data from a recent comprehensive mental health survey, we describe the proportion of women in leadership in a single, large, urban paramedic service in Ontario, Canada. Methods: We distributed an in-person, paper-based survey during the fall 2019 - winter 2020 Continuing Medical Education (CME) sessions. Participating paramedics completed a demographic questionnaire alongside a battery of mental health screening tools. We assessed the demography of the workforce and explored differences in employment classification, provider level (e.g., primary vs. advanced care), and participation in formal leadership roles along self-reported gender lines. Results: Out of 607 paramedics attending CME, we received 600 completed surveys, with 11 excluded for missing data, leaving 589 for analysis and a 97% response rate. Women comprised 40% of the active-duty paramedic workforce, with an average of 8 years of experience. Compared to men, women were more than twice as likely to have a university degree (Odds Ratio [OR] 2.02), but almost half as likely to practice at the Advanced Care Paramedic level (OR 0.61), and somewhat less likely to be employed full-time (OR 0.77). Women were nearly 70% less likely to hold a leadership role in the service compared to men (OR 0.36), occupying just 20% of leadership positions. Conclusion: Although paramedicine is witnessing an encouraging shift in the demography of its workforce with greater participation from women, there is still work to be done, particularly in leadership. Future research should focus on identifying and ameliorating barriers to career advancement among women and other historically underrepresented people.

Preprint ARTICLE | doi:10.20944/preprints202310.0945.v1

Metataxonomic Analysis of Bacterial Diversity in Pigeon Pea after Soaking in Water

Yuni Sine, Donny Widianto, Yekti Asih Purwestri, Byong H. Lee, Widodo Widodo

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: pigeon pea; bacterial diversity; metataxonomic analysis

Online: 16 October 2023 (13:15:21 CEST)

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: With the increase in demand for non-dairy starter cultures and probiotic bacteria as carriers, the use of legumes (also called pulses) as an alternative has gained momentum. In this study, we investigated the diversity of bacterial communities in samples of pigeon pea (Cajanus cajan L. Millsp.) soaked in water for 12 h and 24 h. We soaked 500 g of pigeon pea in sterile distilled water at room temperature (± 25 °C) for 12 h and 24 h; 10 mL of the soaking water was then collected to measure the bacterial diversity using a metataxonomic analysis. The V1–V9 regions on the 16S ribosomal RNA gene were amplified using 27F and 1492R primers under specific polymerase chain reaction conditions for the bacterial identification. Genomic DNA (130 ng) was sequenced on a R9.4 flow cell by Oxford Nanopore Technologies using a GridION sequencer. Library preparations were initiated using a Native Barcoding Kit 24 V14 (SQK-NBD114.24). Primary data were acquired using MinKNOW version 22.05.7. A total of 13 bacterial families and 89 genera were identified in the pigeon pea sample soaked for 12 h; 26 families and 90 genera were identified in the pigeon pea sample soaked for 24 h. Among the bacterial families identified, the five predominant families in both samples were Enterobacteriaceae, Erwiniaceae, Yersiniaceae, Pectobacteriaceae, and Lactobacillaceae. According to the relative abundance of the identified bacterial genera, the following nine genera were predominant in both samples: Enterobacter, Klebsiella, Citrobacter, Pantoea, Kosakonia, Pseudoenterobacter, Pluralibacter, Leclercia, and Kluyvera. At a genus level, a slight increase in the abundance of Klebsiella, Kosakonia, and Pluralibacter and a slight decrease in the abundance of Citrobacter were observed after prolonged incubation from 12 h to 24 h. The values of five diversity indices revealed that the sample soaked in water for 24 h had a richer bacterial abundance and diversity than the 12 h sample. Shannon and Simpson values revealed a higher bacterial diversity in the sample collected at 24 h than the sample collected at 12 h. Species observations and abundance-based coverage estimator (ACE) values demonstrated that the sample collected at 24 h harbored a higher bacterial richness than the sample collected at 12 h. These findings indicated that the bacterial diversity in the pigeon pea samples increased with the soaking time. The bacterial communities during the soaking of the pigeon pea samples were dominated by the Enterobacteriaceae family and Enterobacter genus. The presence of bacterial genera such as Lacticaseibacillus, Lentilactobacillus, and Secundilactobacillus was notable because of their importance as starter cultures for fermented plant-based milk products, including pigeon pea beverages for lactose-intolerant individuals or individuals with malnutrition.

Preprint ARTICLE | doi:10.20944/preprints202305.1425.v1

Anaemia in Preschool-Aged Children in DR. Congo: Finding from a Nationally Representative Survey

Ngianga II (Shadrack) Kandala

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Haemoglobin; Anaemia; Dietary, Diversity; Random-effect

Online: 19 May 2023 (10:06:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201808.0015.v1

A Novel Device-Free Counting Method Based on Channel Status Information

Junhuai Li, Pengjia Tu, Huaijun Wang, Kan Wang, Lei Yu

Subject: Computer Science And Mathematics, Computer Science Keywords: wavelet transform; covariance matrix; spatial diversity; frequency diversity; robustness

Online: 1 August 2018 (10:10:13 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.1642.v1

Shrews (Soricidae) and Viruses Identified in Shrews

Huan-Yu Gong, Rui-Xu Chen, Su-Mei Tan, Xiu Wang, Ji-Ming Chen, Yuan-Long Zhang, Ming Liao

Subject: Biology And Life Sciences, Virology Keywords: shrew; virus; diversity; domestic animal; human

Online: 21 December 2023 (10:25:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0419.v1

Enhancing Tree Health through Improved Microbial Biodiversity in Soil

Vinesh Prasad, Daniel Cole

Subject: Environmental And Earth Sciences, Soil Science Keywords: soil health; mirobilogy; diversity; tree health

Online: 8 October 2023 (04:33:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0860.v1

Equity, Diversity, and Inclusion Strategies in Engineering and Computer Science

Adan Amer, Gaganpreet Sidhu, Maria Isabel Ramirez Alvarez, Juan Antonio Lopez Ramos, Seshasai Srinivasan

Subject: Computer Science And Mathematics, Software Keywords: Equity; Diversity; Inclusion; strategies; priority areas

Online: 12 December 2023 (10:23:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0414.v1

Genotypic and Epidemiologic Profiles of Giardia duodenalis in Four Brazilian Biogeographic Regions

Deiviane Calegar, Beatriz Nunes, Kerla Monteiro, Polyanna Bacelar, Brenda Evangelista, Mayron Moraes, Jurecir Silva, Jéssica Santos, Márcio Bóia, Lauren Jaeger, Filipe Anibal Carvalho-Costa

Subject: Medicine And Pharmacology, Tropical Medicine Keywords: Giardia duodenalis; Assemblages; Epidemiology; Genetic diversity

Online: 27 January 2022 (11:18:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0206.v1

Testing White’s Floristic Impoverishment Hypothesis in the Forest-Savanna Transition Zones of Africa

Mathew Rees, John L. Godlee, David J. Harris, Casey M. Ryan, Kyle G. Dexter

Subject: Environmental And Earth Sciences, Ecology Keywords: Africa; forest; savanna; phylogenetic; diversity; transition

Online: 4 May 2023 (06:04:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1032.v1

Agronomic and Economic Evaluation of Tree Species in Agroforestry Systems with Cocoa (Theobroma cacao L.) in Amazonas

Nixon Huaman Haro, Jerry Bryan Lopezhaya Mendoza, Angel James Medina Medina, Jhon Antony Zabaleta Santisteban, Daniel Tineo Flores, Lily Juarez-Contreras, Malluri Goñas, Manuel Oliva-Cruz

Subject: Biology And Life Sciences, Forestry Keywords: Peru; cocoa; diversity; agroforestry systems; economic analysis

Online: 18 March 2024 (16:12:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints201911.0349.v1

Genetic Diversity of Bemisia tabaci (Gennadius) (Hemiptera:Aleyrodidae) Colonizing Sweet Potato and Cassava in South Sudan

Beatrice Clarence Misaka, Everlyne N. Wosula, Philip W. Marchelo-d'Ragga, Trine Hvoslef-Eide, James Peter Legg

Subject: Biology And Life Sciences, Forestry Keywords: Bemisia tabaci; genetic diversity; distribution; haplotype

Online: 28 November 2019 (03:24:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202303.0481.v1

IRAPs in Combination with Highly Informative ISSRs Confer Effective Potentials for Genetic Diversity and Fidelity Assessment in Rhododendron

Sulin Wen, Hong Zhao, Manying Zhang, Guang Qiao, Xiaohui Shen

Subject: Biology And Life Sciences, Plant Sciences Keywords: genetic fidelity; genetic diversity; IRAP; ISSR; Rhododendron

Online: 28 March 2023 (09:56:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0283.v1

The Functional Biogeography of eDNA Metacommunities in the Post-fire Landscape of the Angeles National Forest

Savanah Senn, Sharmodeep Bhattacharyya, Gerald Presley, Anne E. Taylor, Jillian Ford, Brandon Jasinski, Yekaterina Badalova

Subject: Environmental And Earth Sciences, Environmental Science Keywords: molecular ecology; functional diversity; DNA sequencing

Online: 28 April 2022 (10:31:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202002.0249.v1

The Yeast Atlas of Appalachia: Species and Phenotypic Diversity of Herbicide Resistance in Wild Yeast

Jordan B. Barney, Matthew J. Winans, Catherine B. Blackwood, Amaruy Pupo, Jennifer E. G. Gallagher

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Fungal diversity; Saccharomyces; genetic diversity; glyphosate-based herbicides; copper-based fungicides; RoundUp Ready™ corn; phylogenetics

Online: 17 February 2020 (15:37:11 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202305.0879.v1

Glucosinolate Diversity Analysis in Choy sum (Brassica rapa subsp chinensis var parachinensis) Germplasms for Functional Foods Breeding

Seong-Hoon Kim, Parthiban Subramanian, Bum-Soo Hahn

Subject: Biology And Life Sciences, Food Science And Technology Keywords: glucosinolate; germplasm; breeding; anticancer; genebank; diversity

Online: 12 May 2023 (04:27:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0692.v1

Functional and Species Diversity across Disturbance Levels and Environmental Variables in Yayu Coffee Forest Biosphere Reserve, Southwest Ethiopia

Ferede Abuye Jeldu, Motuma Tolera Feyisa, Teshale Woldeamanuel

Subject: Biology And Life Sciences, Forestry Keywords: biosphere reserve; disturbance; functional diversity; zonation

Online: 11 July 2023 (10:55:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1956.v1

Building a Teleost Fish Traceability Program Based on Genetic Data from Pacific Panama Fish Markets

Edgardo Enrique Díaz-Ferguson, Magaly Sánchez De Chial, Maribel Arlene González Torres, Edgardo A. Muñoz T., Magister Olga Chen, Ovidio Durán, Angel Javier Vega, Carlos Ramos Delgado

Subject: Biology And Life Sciences, Aquatic Science Keywords: COI; teleostei; nucleotide diversity; haplotypic diversity; qPCR; demographic history; environmental DNA

Online: 28 June 2023 (07:37:41 CEST)

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Fish tissue samples from 203 adult individuals were collected in the main ports and markets of the Pacific coast of Panama. Molecular identification based on cytochrome oxidase I gene segment of all species was verified GENBANK reference sequences. 34 species from 14 families (Ariidae, Lutjanidae, Caranjidae, Scianidae, Centropomidae, Serranidae, Scombridae, Sphyraenidae, Haemulidae, Gerreidae, Stromotidae, Lobotidae, Malacanthidae, Mugilidae) were identified at species molecular level from 164 sequences. Also, three Caribbean species were molecularly identified among the analyzed samples (Mycteroperca xenarcha, Paralonchurus brasilensis and Lobotes surinamensis). Species diversity was slightly higher in the Gulf of Panama than in the Gulf of Chiriquí. For species with 5 or more individual sequences, genetic diversity and genetic connectivity parameters such as: total number of haplotypes (H), haplotype diversity (Hd), and nucleotide diversity (π) were calculated. Overall, pelagic-migratory species showed higher values of genetic diversity than coastal and estuarine species with some exceptions. Connectivity between Gulf areas was compared using values of genetic distances and genetic differentiation (Fst). High level of connectivity observed between Gulf of Chiriqui and Gulf of Montijo indicates the existence of a single stock in that area for the following species: Scomberomorus sierra, Caranx caninus and Lutjanus guttatus. Demographic history of the most common species was examined using Tajima’s D values suggesting population expansion for two snapper species, L. peru and L. argentiventris, having significant and higher values. Another important contribution from this research was the production of primers and dual labeled probes for environmental DNA detection using qPCR for the five most abundant species (spotted rose snapper, yellow snapper, green jack, Pacific crevalle jack and the Pacific sierra fish). These markers represent a new set of tools for environmental DNA (eDNA) detection and molecular traceability of three commercially important fish species along the supply chain including landing sites and markets of the main fishery areas.

Preprint ARTICLE | doi:10.20944/preprints202206.0328.v1

Assessing the Spatiotemporal Relationship between Coastal Habitats and Fish Assemblages at Two Neotropical Estuaries of the Mexican Pacific

Victor Muro, Felipe Amezcua, Georgina Ramírez-Ortiz, Francisco Flores-de-Santiago, Felipe Amezcua-Linares, Yareli Hernandez

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: mangrove forests; Marine Protected Areas; α-diversity; β-diversity; multivariate analyses

Online: 24 June 2022 (03:28:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0029.v1

Genomics of Re-emergent Aeromonas salmonicida in Atlantic Salmon Outbreaks

Marcos Godoy, Marco Montes de Oca, Rudy Suarez, Alexis Martinez, Juan Pablo Pontigo, Diego Caro, Karina Kusch, Harry Bohle, Molly Kibenge, Frederick Kibenge, Sion Bayliss

Subject: Biology And Life Sciences, Aquatic Science Keywords: furunculosis; Aeromonas salmonicida; Salmo salar; genomic diversity

Online: 1 December 2023 (05:31:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints201903.0057.v1

Ruderal Plants in Urban and Sub-Urban Walls and Roofs

Alperen Meral, Emrah Yalçınalp

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Urbanization, biological diversity, ruderal vegetation, roof, walls

Online: 5 March 2019 (11:34:03 CET)

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Objective: Main purpose of this study is to identify the ruderal plant species which spontaneously grows on the wall and roof surfaces in urban and sub-urban areas due to their limited ecological needs and to contribute to the creating of the sustainable green areas in urban environments by understanding the parameters that ruderals depend on while they require little maintenance and irrigation support if not no. Material and Methods: The main material of this study is the ruderal plants which were collected from totally 60 walls and 36 roof surfaces within six districts of Trabzon city –Akçaabat, Arsin, Çaykara, Of, Ortahisar and Yomra in Turkey. From these 96 habitats, 1540 plants samples form the walls and 448 plant samples from the roofs were collected. All the plant samples collected from the research area were identified in the herbarium of the faculty of forestry in Karadeniz Technical University. Apart from this, parametres affecting coverage rate of common species on three different habitats were analysed.Results: It was found that 448 samples from the roof surfaces distributed into 61 species while 1540 samples from the walls distributed into 196 species. Plus, according to the analyses, 28 species were found on all three different habitats. As a result of the observations, measurements and analyses, it is clear from the study that coverage rate of the plant species depends on anthropogenic interaction, daylight period and depth of the media but there is no relation with the number of the species on the surfaces.Conclusion: Ruderal plants are definitely important to study on, if the world wants the term sustainability to find its real meaning as they require nearly nothing to grow in hard conditions. In urban life, maintenance is getting more and more expensive for green areas in urban life and this makes it difficult for them to survive especially when cities have limited budget on this, which has often occurred all over the world recently. There is no doubt that ruderal plants offer a great opportunity for modern era urban areas with their limited needs to grow in hard conditions. Furthermore, when thinking about the fact a serious amount of the ruderal plants detected on all three basic habitats has a great landscape plant characteristics, the approaches to their usage in urban areas are really critical.

Preprint ARTICLE | doi:10.20944/preprints202111.0147.v1

Diversity and Career Progression in Dental School Staff in the UK and Ireland

Khaleel Shazada, Zoe Freeman, Hawa Fathi, Paula Waterhouse, Chris Penlington

Subject: Social Sciences, Education Keywords: Diversity; Equality; Dental Education; Career Progression; Higher Education

Online: 8 November 2021 (13:40:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints201705.0145.v1

Spatial Pattern Dynamics Analysis in Coastal Area Using Spatial Metric in Pekalongan, Indonesia

Ali Wijaya, Cahyono Susetyo, Azillatin Q. Diny, Danika H. Nabila, Rahel P. Pamungkas, Mohammad Hadikunnuha, Nursakti A. Pratomoatmojo

Subject: Environmental And Earth Sciences, Environmental Science Keywords: spatial pattern; land use; spatial metric; aggregation; diversity

Online: 19 May 2017 (08:43:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1261.v1

Fungal Diversity and Distribution in the Biodiversity Hotspots of the Western Himalayas

Neelesh Yadav, Shrey Rakholia, Naseem Ali, Reuven Yosef

Subject: Biology And Life Sciences, Life Sciences Keywords: Fungi; Diversity; Western Himalayan region; PCA; ANOVA

Online: 19 September 2023 (08:28:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201907.0346.v1

Biological Hotspot larva Ikan Diperairan Estuari Desa Timbulsoko, Demak

Hanityo Nugroho, Sutrisno Anggoro , Ita Widowati

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: diversity; ecology; fishes; geoscience

Online: 31 July 2019 (04:40:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0308.v1

Effects of Different Feeding Patterns on the Rumen Bacterial Community of Tan Lambs, based on High-Throughput Sequencing of 16S rDNA Amplicons

Lili Zhang, Yanliang Bi, Jie Zhang, Zilin Fu, Xiaofeng Xu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Alpha diversity; Beta diversity; Mutton quality; Pasture-fed; Pen-fed; Tan lambs

Online: 13 April 2023 (08:01:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201902.0018.v2

Comparing Measures of Community Lineage Diversity across North American Forests

Kyle G. Dexter, Ricardo A. Segovia, Andy Griffiths

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: temperate forests; species richness; lineage diversity; phylogenetic diversity; United States; trees; TILD

Online: 13 February 2019 (10:15:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0283.v1

Geomorphology and Altitude Effects on Diversity and Structure of Vanishing Montane Forest of Southern Ecuador

Omar Cabrera, Angel Benítez, Nixon Cumbicus, Carlos Naranjo, Pablo Ramón, Fani Tinitana, Adrián Escudero

Subject: Biology And Life Sciences, Forestry Keywords: alpha diversity; beta diversity; floristic arrangement; geological landscapes; geomorphology; Ecuador; mountain forests

Online: 15 October 2018 (06:19:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0127.v1

The Family Fabaceae in northeastern Mexico (Subfamily Caesalpinioideae, Mimosoideae Clade, Tribes Mimoseae, Acacieae, and Ingeae)

Eduardo Estrada-Castillón, José Ángel Villarreal-Quintanilla, Gerardo Cuéllar-Rodríguez, Juan Antonio Encina-Domínguez, José Guadalupe Martínez-Ávalos, Arturo Mora-Olivo, Jaime Sánchez-Salas

Subject: Biology And Life Sciences, Plant Sciences Keywords: Leguminosae; Clade Mimosoideae; taxonomy; diversity; northeastern Mexico

Online: 3 January 2024 (03:54:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0505.v1

Tomato Defence Suppression is a Common Trait in Tetranychus urticae Collected from Field-Grown Tomatoes

Jéssica Teodoro-Paulo, Carlos Fernandes, Lin Dong, Sara Magalhães, Alison B. Duncan, Juan M. Alba, Merijn R. Kant

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: phylogeny; salivary effectors; effector 84; genetic diversity

Online: 28 February 2023 (04:44:34 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints201912.0176.v1

A Framework for Maximising the Capture of Genetic Diversity in Sampling for ex situ Conservation

Linda Neaves

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: conservation; genetic diversity; decision-making; ex situ

Online: 12 December 2019 (12:43:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints201803.0215.v1

Estimation of Genetic Parameters and Prediction of Genotypic Values in Common Beans Using Mixed Models

Jessica Delfini, Vania Moda Cirino, Claudete de Fátima Ruas, Paulo Mauricio Ruas, José dos Santos Neto, Leandro Simões Azeredo Gonçalves

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Phaseolus vulgaris L.; REML/BLUP; genetic diversity

Online: 26 March 2018 (10:35:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1305.v1

Dynamics in soil microbial community during forest succession depend on soil properties in the karst region of Southwest China

Qin Peng, Min Song, Feng Wang, Hu Du, Tong Qing Song, Hui Jun Chen, Fu Ping Zeng, Wan Xia Peng

Subject: Environmental And Earth Sciences, Ecology Keywords: Soil microbial community; Diversity; Occurrence; Succession; Karst forest

Online: 17 January 2024 (15:53:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202206.0178.v1

Phylogeographic and Bioclimatic Determinants of the Dorsal Pattern Polymorphism in the Italian Wall Lizard, Podarcis siculus

Francesco Gallozzi, Paolo Colangelo, Gabriele Senczuk, Riccardo Castiglia

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Citizen science; Colour pattern; geographic diversity; phylogeography

Online: 13 June 2022 (09:55:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0186.v1

Fungi in Microbial Culture Collections and Their Metabolites

Alexander Vasilenko, Natalya Ivanushkina, Galina Kochkina, Svetlana Ozerskaya

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: collections of microorganisms; data bases; fungal diversity; metabolites

Online: 13 May 2022 (09:42:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0712.v1

A Preliminary Survey of the Avifaunal Diversity in Pampaimadu Premise of the University of Vavuniya, Vavuniya, Sri Lanka

Keerthanaram Thanabalasingam, Wijerathna Poornima, Sanjeewani Nimalka

Subject: Environmental And Earth Sciences, Environmental Science Keywords: avifauna; checklist; habitat variation; species diversity; Vavuniya

Online: 13 July 2023 (07:11:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202101.0236.v1

Facing Racism and Sexism in Science by Fighting against Social Implicit Bias: A Latin and Black Woman Perspective.

Karin Calaza, Fatima Erthal, Mirtes Pereira, Kita Macario, Veronica Daflon, Isabel David, Helena Castro, Maria Vargas, Laura Martins, Jasmin Stariolo, Eliane Volchan, Leticia Oliveira

Subject: Social Sciences, Psychology Keywords: implict bias; gender disparity; diversity; racial dispariry

Online: 12 January 2021 (17:51:36 CET)

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Preprint ARTICLE | doi:10.20944/preprints202001.0060.v1

Doubling the Accuracy of Indoor Location: Frequency Diversity

Berthold Horn

Subject: Engineering, Electrical And Electronic Engineering Keywords: indoor location; fine time measurement; round trip time; FTM; RTT; IEEE 802.11mc; IEEE 802.11-2016; time diversity; spatial diversity; bandwidth diversity; frequency diversity; Bayesian grid; observation model; transition model

Online: 8 January 2020 (04:18:02 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.0432.v1

Inclusion of Underrepresented Populations in Cardiovascular Genetics and Epidemiology

Elias Chappell, Laura Arbour, Zachary Laksman

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Cardiovascular Epidemiology; Cardiogenetics; Underrepresented Populations; Diversity; Genetics; Genomics

Online: 5 January 2024 (02:10:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202104.0659.v1

A Checklist of Indigenous Ichthyofauna in the Dhanu River and Surrounding Wetlands of Bangladesh: Current Status, Population Trends and Threats

Debasish Pandit, Shuva Saha, Mrityunjoy Kunda, Ahmed Harun-Al-Rashid

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Beel, conservation, fish diversity, IUCN, native fishes

Online: 26 April 2021 (12:06:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0444.v1

Behavioural Impact of Captive Management Changes in Three Species of Testudinidae

Jessica T. Turner, Alexandra L. Whittaker, David McLelland

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: reptile; tortoise; welfare; diversity index; behaviour; enrichment; ethogram

Online: 28 October 2022 (08:28:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201808.0526.v1

Faba bean (Vicia Faba L.) Germplasm Collection and its Diversity in Oman

Nadiya AlSaady, Saleem Nadaf, Ali Al-Lawati, Saleh Al-Hinai

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Landraces, indigenous, accession, seed characters, diversity, faba bean

Online: 30 August 2018 (12:01:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0913.v1

Dynamic of Agronomic Characteristics and Plant Diversity in Lemon Verbena (Aloysia citrodora Paláu.) Cultivation in Greece

Aikaterini Molla, Alexandra Solomou, Miltiadis Tziouvalekas, Alexios Lolas, Elpiniki Skoufogianni

Subject: Environmental And Earth Sciences, Soil Science Keywords: Medicinal plant; LAI; Yield; Plant diversity; Environment; Sustainability; Greece

Online: 13 December 2023 (10:51:30 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202304.1127.v1

Metformin Therapy Changes Gut Microbiota Alpha-Diversity in COVID-19 Patients with Type 2 Diabetes: The Role of SARS-CoV-2 Variants and Antibiotic Treatment

Pavlo Petakh, Iryna Kamyshna, Valentyn Oksenych, Denis Kainov, Aleksandr Kamyshnyi

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; gut microbiota; diversity; diabetes; metformin

Online: 28 April 2023 (07:06:36 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0243.v1

Lupinus mutabilis Breeding in the Andes of Ecuador, Peru, and Bolivia: A Review

Diego Rodriguez-Ortega, José Luis Zambrano, Santiago Pereira-Lorenzo, Andres Torres, Angel Murillo

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Lupinus mutabilis; genetic diversity; breeding; anthracnose resistance; alkaloids

Online: 5 October 2023 (02:38:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201705.0149.v1

Spatial Pattern Dynamics Analysis at Coastal Area Using Spatial Metric in Pekalongan, Indonesia

Ali Wijaya, Cahyono Susetyo, Azillatin Qisthian Diny, Danika Hudani Nabila, Rahel Putri Pamungkas, Mohammad Hadikunnuha, Nursakti Adhi Pratomoatmojo

Subject: Environmental And Earth Sciences, Environmental Science Keywords: Spatial Pattern; Land Use; Spatial Metric; Aggregation; Diversity

Online: 19 May 2017 (16:21:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0183.v1

The Diversity of Gut Microbiota at Weaning Is Altered in Prolactin Receptor Null Mice

Ivan Luzardo-Ocampo, Ana Luisa Ocampo-Ruiz, José L. Dena-Beltrán, Gonzalo Martínez de la Escalera, Carmen Clapp, Yazmin Macotela

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: bacterial diversity; gut microbiota; prolactin receptor; lactation; weaning

Online: 2 August 2023 (08:46:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0387.v1

Environmental Diversity Assessment to Enhance Attractineness of Eco-tourism in Asir Region, Saudi Arabia

Jamilah Al Mohaya, Mena Elassal

Subject: Social Sciences, Geography, Planning And Development Keywords: environmental diversity; eco-tourism; Asir region; GIS; RS

Online: 17 September 2020 (08:06:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1390.v1

Evaluation of Wild Raspberry (Rubus idaeus L.) Ecotypes from Northeastern Türkiye

Gulce Ilhan

Subject: Biology And Life Sciences, Horticulture Keywords: Raspberry; underutilized fruits; characterization; diversity

Online: 19 January 2024 (03:12:32 CET)

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Located between Asia and Europe, Türkiye is very rich in terms of wild edible fruit diversity. One of the most important wild edible fruits in Türkiye is red raspberries and they widely found in cold to warm temperate regions in the country. The wild raspberries in general have red fruit color but yellow fruited genotypes are evident. A total of 11 wild grown red raspberry genotypes sampled in Northern Anatolia region was used in this study and their sensory, biochemical and antioxidant characteristics were determined. For sensory analysis aroma, taste and juiciness were used as the main criteria to compare ecotypes each other. Biochemical and antioxidant characteristics of ecotypes included SSC (Soluble Solid Content), vitamin C, organic acids, total anthocyanins (TA), total phenolic content, total flavonoid content and total antioxidant capacity. FRAP (Ferric Reducing Antioxidant Power) assay was used to determine antioxidant capacity. There were significant differences among ecotypes for most of the searched parameters even they found similar growing condition. The ecotypes had fruit weight between 1.04 g and 1.33 g, and fruit Chroma value between 26.11 and 33.70. Vitamin C content of ecotypes were quite variable and ranged from 29.3 mg/100 g to 41.4 mg/100 g. The total phenolic content, total anthocyanin content, total flavonoid content was between 164-362 mg gallic acid equivalent/100 g; 17.3-33.3 mg cyanidin-3-glucoside equivalent/100 g and 10.3-17.2 mg quercetin equivalent/100 g, respectively. Citric acid was the dominant organic acid for all genotypes and ranged from 10.3-14.6 g/100 g fresh weight base. According to the results V-4, V-8, V-3 and V-10 had attractive bigger fruits indicate their suitability for fresh consumption. V-3 and V-5 had sweeter fruits indicated their suitability for processing and V-6, V-7 and V-11 had richer for human health promoting compounds (higher total phenolic content and antioxidant capacity), making them suitable for future use as functional foods and as promising sources of natural antioxidants.

Preprint REVIEW | doi:10.20944/preprints202206.0284.v1

Building Consensus around the Assessment and Interpretation of Symbiodiniaceae Diversity

Sarah Davies, Matthew H. Gamache, Lauren I. Howe-Kerr, Nicola G. Kriefall, Andrew C. Baker, Anastazia T. Banaszak, Line K. Bay, Anthony J. Bellantuono, Debashish Bhattacharya, Cheong Xin Chan, Danielle C. Claar, Mary Alice Coffroth, Ross Cunning, Simon K. Davy, Javier del Campo, Erika M. Diaz-Almeyda, Jorg C. Frommlet, Lauren E. Fuess, Raul A. Gonzalez-Pech, Tamar L. Goulet, Kenneth D. Hoadley, Emily J. Howells, Benjamin C. C. Hume, Dustin W. Kemp, Carly, D. Kenkel, Sheila A. Kitchen, Todd C. LaJeunesse, Senjie Lin, Shelby McIlroy, Ryan McMinds, Matthew R. Nitschke, Clinton A. Oakley, Raquel S. Peixoto, Carlos Prada, Hollie M. Putnam, Kate Quigley, Hannah G. Reich, James Davis Reimer, Mauricio Rodriguez-Lanetty, Stephanie Rosalas, Osama S. Saad, Eugenia M. Sampayo, Scott Santos, Eiichi Shoguchi, Edward G. Smith, Michael Stat, Timothy G. Stephens, Marie E. Strader, David J. Suggett, Timothy D. Swain, Cawa Tran, Nikki Traylor-Knowles, Christian R. Voolstra, Mark E. Warner, Virginia M. Weis, Rachel Wright, Tingting Xiang, Hiroshi Yamashita, Maren Ziegler, Adrienne M.S. Correa, John Everett Parkinson

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Symbiodinaceae; genetic diversity; coral symbiosis

Online: 21 June 2022 (04:49:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202108.0397.v1

Simple Method for Assessing Diversity and Dynamics of Microbial Community: Comparison of Dairy Phages from Industrial and Spontaneous Fermentation

Agnieszka Olejnik-Schmidt, Bernadeta Pietrzak, Iwona Kawacka, Klaudia Malak, Weronika Wawrzyniak, Marcin Schmidt

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: phage; dairy; Lactococcus; diversity; community

Online: 19 August 2021 (10:30:53 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202008.0021.v1

Safe Fieldwork Strategies for At-Risk Individuals

Amelia-Juliette Demery, Monique Pipkin

Subject: Social Sciences, Safety Research Keywords: diversity; inclusion; equity; fieldwork; research

Online: 2 August 2020 (11:55:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201807.0366.v1

Does Islam Practice Tolerance? Some Notes from Quranic Perspective

Abur Hamdi Usman

Subject: Social Sciences, Religion Keywords: Tolerance; Religion; Islam; Society; Diversity

Online: 19 July 2018 (15:43:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0179.v1

Economic Diversity and the Resource Curse: A Dynamic Panel Model

Fred Olayele, Kwok Soo

Subject: Business, Economics And Management, Economics Keywords: Diversity; resource curse; economic growth.

Online: 13 April 2018 (14:18:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201801.0247.v1

Species Diversity of Oak Stands and Its Significance for Drought Resistance

Jan Kotlarz, Sylwia Nasiłowska, Karol Rotchimmel, Katarzyna Kubiak

Subject: Environmental And Earth Sciences, Environmental Science Keywords: drought; diversity; oaks; remote sensing

Online: 26 January 2018 (04:52:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints201611.0116.v1

Evaluation of Diversification Techniques for Legal Information Retrieval

Marios Koniaris, Ioannis Anagnostopoulos, Yannis Vassiliou

Subject: Computer Science And Mathematics, Information Systems Keywords: diversity; algorithms; legal information retrieval

Online: 23 November 2016 (09:50:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0849.v1

How Can Religious Fractionalization and Polarization Inform Us at the County Level in Post-Communist Romania?

Marina-Alexandra Rotaru, Remus Cretan, Ioan Sebastian Jucu, Ana-Neli Ianas, Marcel Torok-Oance

Subject: Social Sciences, Religion Keywords: religious diversity; quantitative methodology; fractionalization; polarization; counties; Romania

Online: 14 March 2024 (10:34:55 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0026.v1

Molecular Prevalence, Genetic Diversity, and Tissue Tropism of Bartonella Species in Small Mammals from Yunnan Province, China

Pei-Yu Han, Fen-Hui Xu, Jia-Wei Tian, Jun-Ying Zhao, Ze Yang, Wei Kong, Bo Wang, Li-Jun Guo, Yun-Zhi Zhang

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Bartonella; small mammals; genetic diversity; evolution; tissue tropism

Online: 1 April 2024 (17:07:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0385.v1

mySORT: A Web Framework by using Deconvolution Approach to Estimating Immune Cell Composition from Complex Tissues

Shu-Hwa Chen, Bo-Yi Yu, Wen-Yu Kuo, Ya-Bo Lin, Sheng-Yao Su, I-Hsuan Lu, Chung-Yen Lin

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Cancer; Immunotherapy; Deconvolution; Alpha diversity; Beta diversity; Precision medicine; Microenvironment; Single-cell RNA sequencing

Online: 13 November 2020 (14:18:40 CET)

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Cancer immunotherapy reaches a remarkable achievement in various cancer types and brings new possibilities to improve cancer patients’ long-term survival. However, outcomes vary from case to case, and the present protocol benefits a small fraction of patients. One notable factor is the tumor microenvironment, especially the immune cell components, that may reflect the immune response's status quo on site. Thus, understanding the content of infiltrating immune cells in tumors is not only for research interesting but also a crucial subject toward precision medicine. We implement an algorithm for resolving relative proportions of twenty-one immune cell subclasses from a human tissue profiled transcriptome by microarray technology to reach the goal above. By selecting gene features and then adopting ?-Support Vector Regression, we can construct a deconvolution model and resolve the immune cell context. The excellent consistency between the estimated values and the correct immune-cell composition further demonstrates this approach provides a more natural alternative to revealing samples' immune cell content and reliable results like recent single-cell technologies. Based on this algorithm, the web-based deconvolution tool implemented named mySORT provides a user-friendly interface for estimating the immune cell content by uploading gene expression profiling. We also present comprehensive visualization 2D/3D plots in mySORT so that users can easily make a comparison between different samples. Finally, we synthesized pseudo-bulk expression data from single-cell transcriptomic datasets of 17 melanoma and 16 head and neck cancer patients. The deconvolution results of microarray-based data in the previous study and synthetic pseudo-bulk data all proved the excellent performance of mySORT. We believe that mySORT can help researchers in all fields easily understand complex immune microenvironment. The website of mySORT is freely accessible on https://symbiosis.iis.sinica.edu.tw/mySORT/.

Preprint ARTICLE | doi:10.20944/preprints201811.0627.v1

BIOFACQUIM: A Mexican Compound Database of Natural Products

B. Angélica Pilón-Jiménez, Fernanda I. Saldívar-González, Bárbara I. Díaz-Eufracio, José L. Medina-Franco

Subject: Chemistry And Materials Science, Theoretical Chemistry Keywords: chemical space; chemical data set; chemoinformatics; consensus diversity plot; drug discovery; molecular diversity; visualization

Online: 30 November 2018 (10:06:15 CET)

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Preprint REVIEW | doi:10.20944/preprints202010.0149.v1

Advances in Melon Breeding (Cucumis melo L.): An Update

Hari Kesh, Prashant Kaushik

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: breeding; diversity; genetic engineering; genomics; male sterility; melon; QTLs

Online: 7 October 2020 (09:22:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1528.v1

A Systematic Assessment of Leishmania donovani Infection in Domestic and Wild Animal Reservoir Hosts of Zoonotic Visceral Leishmaniasis

Gajala Deethamvali Ghouse Peer, Anjali Priyadarshini, Archana Gupta, Arpana Vibhuti, Elcio Leal, Antonio Charlys da Costa, Carlos Prudencio, Chung-Ming Chang, V.Samuel Raj, Ramendra Pandey

Subject: Biology And Life Sciences, Parasitology Keywords: Leishmania donovani; Geographical; Temporal diversity; Epidemic; Transmission cycle

Online: 21 June 2023 (10:31:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0613.v1

Assessment of Genetic Diversity in Winged Bean [Psophocarpus Tetragonolobus (L.) DC.] Accessions Using Morphological Traits and Microsatellite Markers

Temitope Esther Olatunde, Adeolu Moses Adebayo, Ayomide Ajoke Bhadmus, Idris Ishola Adejumobi, Olaniyi Oyatomi, Benjamin Faloye, Michael Abberton

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Accessions; genetic diversity; morphological traits; SSRs; winged bean

Online: 8 June 2023 (09:25:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0924.v1

Tree Diversity and Its Ecological Importance Value in Silvopas-Toral Systems: A Study along Elevational Gradients in the Su-Maco Biosphere Reserve, Ecuadorian Amazon

Bolier Torres, Robinson J. Herrera-Feijoo, Alexandra Torres Navarrete, Carlos Bravo, Antón García

Subject: Environmental And Earth Sciences, Sustainable Science And Technology Keywords: Trees in grasslands; Diversity indices; Endangered species; Ecuadorian Amazon

Online: 11 January 2024 (12:01:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.0851.v1

Impacts of Combined Exposure to Glyphosate and Diquat on Microbial Community Structure and Diversity in a Lateritic Paddy Soil

Xiaoyu He, Chunyuan Wu, Huadong Tan, Xiao Deng, Yi Li

Subject: Environmental And Earth Sciences, Environmental Science Keywords: Herbicides; mixed toxicity; microbial diversity; microbial community structure

Online: 24 April 2023 (10:49:58 CEST)

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Preprint REVIEW | doi:10.20944/preprints201911.0286.v1

Indigenous Pig Genetic Resources in Southern Africa: Progress and Prospects

Tinyiko Edward Halimani, Obvious Mapiye, Tawanda Marandure, Diedre Januarie, Venancio Edward Imbayarwo Chikosi, Kennedy Dzama

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: diversity; conservation; animal genetic resources; indigenous pigs; southern Africa

Online: 24 November 2019 (14:47:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.1471.v1

Diversity and Evolution of the MHC Class II DRB Gene in an Alpine Ungulate, the Siberian Ibex (Capra sibirica), Experienced a Demographic Fluctuation in China

Pei-Pei Dong, Rui-Rui Wang, Shamshidin Abduriyim

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Mountain ungulate; MHC DRB gene; genetic diversity; population fluctuation

Online: 21 July 2023 (09:13:12 CEST)

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The major histocompatibility complex (MHC) genes are the most polymorphic genes in vertebrates, and their proteins play a critical role in adaptive immunity for defense against a variety of pathogens. MHC diversity was lost in many species after experiencing a decline in size. To understand the variation and evolution of MHC genes in the Siberian ibex, Capra sibirica, which has undergone a population decline, we analyzed the variation of the second exon of MHC class II DRB genes in samples collected from five geographic localities in Xinjiang, China, that belong to three diverged mitochondrial clades. Consequently, we identified a total of 26 putative functional alleles (PFAs) with 260 bp in length from 43 individuals, and found one (for 27 individuals) to three (for 5 individuals) PFAs per individual, indicating the presence of one or two DRB loci per haploid genome. The Casi-DRB1*16 was the most frequently occurring PFA, Casi-DRB1*22 came after found in only seven individuals, 14 PFAs occurred once (7 PFAs twice), implying high frequency of rare PFAs. Interestingly, more than half of the (15) PFAs were specific to clade I, only one and three PFAs were specific to clades II and III, respectively. So, we assume that the polygamy and sextual segregation nature of this species likely contributed to the allelic diversity of DRB genes. Genetic diversity indices showed that PFAs of clade II were lower in nucleotide, amino acid, and supertype diversity compared to those of the other two clades. The way of allele sharing and FST values between three clades were to some extent in agreement with pattern observed in mitochondrial DNA divergence. In addition, recombination analyses revealed no evidence for significant signatures of recombination events. Alleles shared by clades III and the other two clades diverged 6 million years ago, and systematic neighbor grids showed cross-species polymorphisms. Together with the PAML and MEME analyses, the results indicated that the DRB gene in C. sibirica evolved under balancing and positive selection. However, by comparison, it can be clearly seen that different populations were under different selective pressures. Our results are valuable in understanding the diversity and evolution of the DRB gene in a mountain living C. sibirica and in making decisions on future long-term protection strategies.

Preprint ARTICLE | doi:10.20944/preprints202205.0157.v1

Low Intra-Host and Inter-Host Genetic Diversity of Carnivore Protoparvovirus 1 in Domestic Cats During A Feline Panleukopenia Outbreak

Xiuwan Wang, Maura Carrai, Kate Van Brussel, Shuo Feng, Julia A. Beatty, Mang Shi, Edward C. Holmes, Jun Li, Vanessa R. Barrs

Subject: Biology And Life Sciences, Virology Keywords: feline; panleukopenia; canine parvovirus; metagenomics; diversity; intra-host

Online: 12 May 2022 (04:07:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0305.v1

Distance Only Brings You Closer: Application of ISSR Markers to Analyze Molecular Relationships in Roses (Rosa spp.) - The Symbol of Love

Tanay Joshi, Santosh Kumar, Lalit Arya, Amritbir Riar

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: ISSR; Rosa spp.; DNA fingerprinting; genotypes; genetic diversity

Online: 13 May 2021 (14:01:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.0895.v1

Circadian Rhythm Perturbation Aggravates Gut Microbiota Dysbiosis in Dextran Sulfate Sodium-Induced Colitis in Mice

Joseph Amara, Tarek Itani, Joelle Hajal, Jules-Joel Bakhos, Youakim Saliba, Saied A. Aboushanab, Elena G. Kovaleva, Nassim Fares, Alicia C. Mondragon, Jose Manuel miranda lopez

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: DSS-induced colitis; Dysbiosis; Circadian rhythm; Bacterial diversity; Microbiota; Gut

Online: 11 January 2024 (11:27:26 CET)

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Circadian rhythm disruption is increasingly considered an environmental risk factor for the development and exacerbation of inflammatory bowel disease. We have reported in a previous study that nychthemeral dysregulation is associated with an increase in intestinal barrier permeability and inflammation in mice with dextran sulfate sodium (DSS)-induced colitis. To investigate the effect of circadian rhythm disruption on the composition and diversity of the gut microbiota (GM). Sixty male C57BL/6J mice were divided initially to two groups, the shifted group (n=30) exposed to circadian shifts for three months and the non-shifted group (n=30) under a normal light-dark cycle. The mice of the shifted group were cyclically housed for five days under the normal 12:12 hour light-dark cycle followed by another five days under reversed light-dark cycle. At the end of the three months a colitis was induced by 2 % DSS given in the drinking water of 30 mice. Animals were then divided into four groups (n=15 per group), sham group non-shifted (Sham-NS), sham group shifted (Sham-S) and DSS non-shifted (DSS-NS) and DSS shifted (DSS-S). Fecal samples were collected from rectal content to investigate changes in GM composition by DNA extraction followed by high-throughput sequencing of the bacterial 16S rRNA gene. The mouse GM was dominated by three phyla: Firmicutes, Bacteroidetes and Actinobacteria. The Firmicutes/Bacteroidetes ratio decreased in mice with induced colitis. The richness and diversity of the GM were reduced in the colitis group, especially in the group with inverted circadian rhythm. Moreover, GM composition was also modified in the inverted circadian rhythm group, with an increase in Alloprevotella, Turicibacter, Bacteroides and Streptococcus genera. Circadian rhythm inversion exacerbates GM dysbiosis to a less rich and diversified extent in a DSS-induced colitis model. These findings shows a possible interplay between circadian rhythm disruption, GM dynamics, and colitis pathogenesis.

Preprint ARTICLE | doi:10.20944/preprints202308.0101.v1

Navigating the Path to Success: Insights for Aspiring Female Leaders in Academia

Wafa Hozien

Subject: Social Sciences, Education Keywords: female; women; academia; higher education; leadership; gender equality; diversity

Online: 2 August 2023 (02:26:53 CEST)

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Preprint REVIEW | doi:10.20944/preprints202203.0295.v1

Genetic Diversity of Schistosoma Haematobium in Sub-Saharan Africa: A Systematic Review

Rabecca Tembo, Panji Nkhoma, Mildred Zulu, Florence Mwaba, John Yabe, Hikabasa Halwiindi, Moses Kamwela, King S. Nalubamba, Chummy S. Sikasunge, Andrew M. Phiri

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Urinary schistosomiasis; Schistosoma haematobium; sub-Saharan Africa; Genetic Diversity

Online: 22 March 2022 (04:25:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints201802.0090.v1

High-throughput Sequencing Analysis of the Actinobacterial Apatial Diversity in Moonmilk Deposits

Marta Maciejewska, Magdalena Calusinska, Luc Cornet, Delphine Adam, Igor Pessi, Sandrine Malchair, Philippe Delfosse, Denis Baurain, Hazel Barton, Monique Carnol, Sébastien Rigali

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: antibiotics; geomicrobiology; Illumina sequencing; microbiome diversity; Streptomyces; Cave microbiology

Online: 12 February 2018 (16:30:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.0285.v1

Variation in Relative Abundance of Small Mammal species Caught in Two Different Ecosystems And Implicated in the Spread of Emerging Pathogens, in Mali

Abdoulaye Kassoum Koné, Georges Diatta, Doumbo Safiatou Niare, Solimane Ag Atteynine, Maïmouna Coulibaly, Adama Zan Diarra, Issaka Sagara, Abdoulaye Djimdé, Ogobara Doumbo, Mahamadou Ali Thera

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Small mammals; Reservoirs; Pathogenic agents; Transmission; Low diversity; Relative abundance; Mali

Online: 13 April 2023 (03:21:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0016.v1

Species Diversity and Community Structure of Macrobenthos in the Cosmonaut Sea, East Antarctica

Jianfeng Mou, Kun Liu, Yaqin Huang, Junhui Lin, Xuebao He, Shuyi Zhang, Dong Li, Yongcan Zu, Zhihua Chen, Sujing Fu, Heshan Lin, Wenhua Liu

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: macrobenthos; species diversity; community structure; Cosmonaut Se; Environmental factors

Online: 1 November 2023 (04:33:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0897.v1

The Cent Fonts aquifer: an overlooked subterranean biodiversity hotspot in a stygobiont-rich region

Vincent Prié, Cédric Alonso, Claude Bou, Diana Maria Paola Galassi, Pierre Marmonier, Marie-José Dole-Olivier

Subject: Biology And Life Sciences, Aquatic Science Keywords: stygobiont, troglobiont, conservation, karst, subterranean diversity, conservation, water abstraction

Online: 12 December 2023 (13:14:12 CET)

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Preprint REVIEW | doi:10.20944/preprints201811.0434.v1

Demographic Diversity of Food Intake and Prevalence of Kidney Stone Diseases in Indian Continent

Manalee Guha, Hritwick Banerjee, Pubali Mitra, Madhusudan Das

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: food intake; food diversity; kidney stone disease; social epidemiology

Online: 19 November 2018 (09:20:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.0820.v1

Dietary Pattern, Nutrition-Related Knowledge and Attitudes of Working Women in Western Province, Sri Lanka

Ayesha Salwathura, Faruk Ahmed

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: Dietary Pattern; Dietary Diversity Score; Nutrition Knowledge; Nutrition Attitudes

Online: 12 June 2023 (10:13:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0826.v1

SSR Based Genetic Diversity Analysis and Population Structure of Proso millet (Panicum miliaceum L.) in Kazakhstan

Meisam Zargar, Elmira Dyussibayeva, Aidyn Orazov, Aiym Zeinullina, Irina Zhirnova, Gulzat Yessenbekova, Aiman Rysbekova

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: proso millet collection; genetic diversity; SSR markers; agronomic traits

Online: 13 September 2023 (10:37:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0942.v1

Population Genetic Structure of a Viviparous Sand Lizard, the Phrynocephalus forsythii in the Tarim Basin, Xinjiang of China

Jiabao Duan, Luoxue Jiang, Tianying Chen, Wen Zhong, Wei Zhao, Yue Qi, Penghui Guo, You Li

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Phrynocephalus forsythii; microsatellite; genetic diversity; population structure

Online: 14 November 2023 (15:25:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1303.v1

Urban Sustainability in Unconventional Food, Case of Edible Insects in Kinshasa: Diversity and Entomophagy

ADEITO MAVUNDA Célestin, Madjouma Kanda, Fousseni Folega, Demirel Maza-esso BAWA, Marra Dourma, Koffi Akpagana

Subject: Anthropology, Social Sciences Keywords: edible insects; food insecurity; ethnozoology; edible insect diversity; Kinshasa; RD Cong

Online: 19 April 2024 (09:26:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1605.v1

Autoprobiotics in the treatment of patients with colorectal cancer in the early postoperative period

Elena I Ermolenko, Natalia V Baryshnikova, Galina Alekhina, Alexander Zakharenko, Oleg Ten, Victor A Kashchenko, Nadezhda Lavrenova, Olga Gushchina, Timofey Ovchinnikov, Anastasia Morozova, Anastasia Ilina, Alena B. Karaseva, Anna Tsapieva, Nikita Gladyshev, Alexander V. Dmitriev, Alexander Suvorov

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: microbiome, autoprobiotics, Enterococcus spp., Parvomonas micra, alpha diversity, gut dysbiosis, interleukins

Online: 28 February 2024 (08:05:17 CET)

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Introduction: Despite great advances in the treatment of oncological diseases, the development of medical technologies to prevent or reduce complications of therapy, in particular, those associated with surgery and the introduction of antibiotics remains relevant. The aim of the study: to evaluate the effectiveness of the use of autoprobiotics based on indigenous non-pathogenic strains of Enterococcus faecium and Enterococcus hirae in the complex therapy of colorectal cancer (CRC) in the early postoperative period. Materials and methods: A total of 36 patients diagnosed with CRC were enrolled in the study. The study group (Group A) comprised 24 CRC patients who received autoprobiotic therapy in the early postoperative period, while the control group (Group C) included 12 CRC patients without autoprobiotic therapy.Prior to surgery and between days 14-16 post-surgery, comprehensive evaluations were conducted on all patients, encompassing the following: stool and gastroenterological complains analysis. examination of the gut microbiota (bacteriological study, quantitative polymerase chain reaction, metagenome analysis), analysis of interleukins in serum. Results: The use of autoprobiotics led to decrease dyspeptic complaints after surgery, was associated with absence of postoperative complications, did not cause side effects, led to a decrease in the level of pro-inflammatory cytokines (IL-6 and IL-18) in the blood serum. The use of autoprobiotics led to positive changes in the structure of escherichia and enterococci populations, the elimination of Parvomonas micra, Fusobacterium nucleatum, decrease in the quantitative content of Clostridium perfringens, Akkermansia muciniphila. Metagenomic analysis (16S rRNA) revealed an increase in alpha diversity. Conclusion: The administration of autoprobiotics in the postoperative period is highly effective and safe in the comprehensive treatment of colorectal cancer patients. Future studies will allow the discovery of additional fine mechanisms of autoprobiotic therapy and its impact on the digestive, immune, endocrine and neural systems.

Preprint ARTICLE | doi:10.20944/preprints202311.1729.v1

Assessment of Diversity and Genetic Variability of Guava (Psidium guajava) by Means of Microsatellite Markers in Montería, Córdoba-Colombia

Enrique Pardo-Perez, Elizabeth Ramos-Rivero, Luis Causil-Vargas

Subject: Biology And Life Sciences, Life Sciences Keywords: genetic diversity; genetic variability; microsatellites; allele frequencies; population structure

Online: 28 November 2023 (08:08:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0787.v1

A Highly Homogeneous Airborne Fungal Community Around a Copper Open-Pit Mine Reveals a Poor Contribution by the Local Aerosolization of Particles

Sebastián Fuentes-Alburquenque, Victoria Olivencia Suez, Omayra Aguilera, Blanca Águila, Luis Rojas Araya, Dinka Mandakovic

Subject: Environmental And Earth Sciences, Ecology Keywords: microbial community; diversity; air; fungi; bioaerosol

Online: 11 April 2024 (08:03:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.1064.v1

Culture, Gender, and Business

EMMANUEL ADEYEMI

Subject: Arts And Humanities, History Keywords: culture; gender; business; organization; religion; diversity

Online: 18 September 2023 (05:30:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1234.v1

Butterflies of the Neotropical Seasonally Dry Forest of Montes de María (Sucre, Colombia): What do they Tell us about the Intermediate Disturbance Hypothesis?

Yarlenis L Mercado-Gómez, Jorge D Mercado-Gómez, Carlos E Giraldo-Sánchez

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: structure; diversity; anthropic disturbance; conservation; Lepidoptera

Online: 17 May 2023 (10:38:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0560.v1

Microbial Diversity Based on Multifractal Analysis of Metagenomes

Xianhua Xie, Yuanlin Ma, Zuguo Yu, Guosheng Han

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: diversity index; multifractal; metagenome; gut mtagenome

Online: 26 July 2021 (10:04:41 CEST)

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