Luzardo-Ocampo, I.; Ocampo-Ruiz, A.L.; Dena-Beltrán, J.L.; Martínez de la Escalera, G.; Clapp, C.; Macotela, Y. The Diversity of Gut Microbiota at Weaning Is Altered in Prolactin Receptor-Null Mice. Nutrients2023, 15, 3447.
Luzardo-Ocampo, I.; Ocampo-Ruiz, A.L.; Dena-Beltrán, J.L.; Martínez de la Escalera, G.; Clapp, C.; Macotela, Y. The Diversity of Gut Microbiota at Weaning Is Altered in Prolactin Receptor-Null Mice. Nutrients 2023, 15, 3447.
Luzardo-Ocampo, I.; Ocampo-Ruiz, A.L.; Dena-Beltrán, J.L.; Martínez de la Escalera, G.; Clapp, C.; Macotela, Y. The Diversity of Gut Microbiota at Weaning Is Altered in Prolactin Receptor-Null Mice. Nutrients2023, 15, 3447.
Luzardo-Ocampo, I.; Ocampo-Ruiz, A.L.; Dena-Beltrán, J.L.; Martínez de la Escalera, G.; Clapp, C.; Macotela, Y. The Diversity of Gut Microbiota at Weaning Is Altered in Prolactin Receptor-Null Mice. Nutrients 2023, 15, 3447.
Abstract
Maternal milk supports offspring development by providing microbiota, macronutrients, micronutrients, immune factors, and hormones. The hormone prolactin (PRL) is an important component of milk with protective effects against chronic non-communicable diseases (NCDs). Because maternal milk regulates microbiota composition and microbiota protect against NCDs, we aimed to investigate whether PRL regulates gut microbiota in newborn mice. 16SrRNA sequencing of feces and bioinformatics analysis were performed to evaluate gut microbiota in mice null for the PRL receptor (Prlr-KO) at the onset of weaning (postnatal day 21). The normalized colon and cecal weights were higher and lower, respectively, in Prlr-KO mice relative to wild-type mice (Prlr-WT). Relative abundance (Simpson Evenness Index), phylogenetic diversity, and bacterial concentration in gut contents were lower in the absence of the PRL receptor. Eleven bacteria species, out of 470, differed between Prlr-KO and Prlr-WT mice with two bacterial genera (Anaerotruncus and Lachnospiraceae) related to NCD development being the most common in Prlr-KO. Furthermore, a higher metabolism of terpenoids and polyketides was predicted in Prlr-KO mice compared to Prlr-WT mice; these metabolites have antimicrobial properties and are present in microbe-associated pathogenicity. In summary, the absence of the PRL receptor changes intestinal microbiota composition, resulting in lower microbiota abundance and richness, which could contribute to NCD development.
Keywords
bacterial diversity; gut microbiota; prolactin receptor; lactation; weaning
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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