Version 1
: Received: 24 November 2023 / Approved: 27 November 2023 / Online: 28 November 2023 (08:08:48 CET)
How to cite:
Pardo-Perez, E.; Ramos-Rivero, E.; Causil-Vargas, L. Assessment of Diversity and Genetic Variability of Guava (Psidium guajava) by Means of Microsatellite Markers in Montería, Córdoba-Colombia. Preprints2023, 2023111729. https://doi.org/10.20944/preprints202311.1729.v1
Pardo-Perez, E.; Ramos-Rivero, E.; Causil-Vargas, L. Assessment of Diversity and Genetic Variability of Guava (Psidium guajava) by Means of Microsatellite Markers in Montería, Córdoba-Colombia. Preprints 2023, 2023111729. https://doi.org/10.20944/preprints202311.1729.v1
Pardo-Perez, E.; Ramos-Rivero, E.; Causil-Vargas, L. Assessment of Diversity and Genetic Variability of Guava (Psidium guajava) by Means of Microsatellite Markers in Montería, Córdoba-Colombia. Preprints2023, 2023111729. https://doi.org/10.20944/preprints202311.1729.v1
APA Style
Pardo-Perez, E., Ramos-Rivero, E., & Causil-Vargas, L. (2023). Assessment of Diversity and Genetic Variability of Guava (<em>Psidium guajava</em>) by Means of Microsatellite Markers in Montería, Córdoba-Colombia. Preprints. https://doi.org/10.20944/preprints202311.1729.v1
Chicago/Turabian Style
Pardo-Perez, E., Elizabeth Ramos-Rivero and Luis Causil-Vargas. 2023 "Assessment of Diversity and Genetic Variability of Guava (<em>Psidium guajava</em>) by Means of Microsatellite Markers in Montería, Córdoba-Colombia" Preprints. https://doi.org/10.20944/preprints202311.1729.v1
Abstract
Guava (Psidium guajava L.) is a species is widely distributed in Córdoba, despite this, there are few studies that have been carried out to understand its genetic diversity. For this reason, the objective of this research was to evaluate the genetic diversity and variability of guava (Psidium guajava L) using microsatellite markers in Montería, Córdoba-Colombia. Forty-five accessions were evaluated. For DNA extraction, the CTAB 2x extraction protocol was used with modifications. The extracted DNA was amplified by the PCR technique using seven microsatellite markers. A total of 46 alleles were found with an average of 6.5 alleles per locus. A total of 28 private alleles were identified in the populations, possibly fixed by different evolutionary forces. The evaluated population presented an average expected heterozygosity of 0.572 and the observed heterozygosity was lower (0.058). The fixation index presented a total average of 0.906, indicating that in Montería there is an excess of homozygotes. The PIC values allowed us to determine that the markers mPgCIR9, mPgCIR11, mPgCIR13, mPgCIR16, mPgCIR19 and mPgCIR22 are highly informative, while mPgCIR23 is moderately informative. The guava population in Montería presented a low genetic diversity within the subpopulations, which could be related to inbreeding events such as self-pollination..
Keywords
genetic diversity; genetic variability; microsatellites; allele frequencies; population structure
Subject
Biology and Life Sciences, Life Sciences
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
