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Preprint ARTICLE | doi:10.20944/preprints202306.0357.v1

Phosphorylation Mimetic of Myosin Regulatory Light Chain Mitigates Cardiomyopathy-induced Myofilament Impairment in Mouse Models of RCM and DCM

Katarzyna Kazmierczak, Jingsheng Liang, Luis Guillermo Maura, Natissa Kelsey Scott, Danuta Szczesna-Cordary

Subject: Biology And Life Sciences, Life Sciences Keywords: Cardiomyopathy; myosin RLC; myosin ELC; N-terminal protein modification, S15D-RLC phosphomimetic, super-relaxed state (SRX); Tg mice

Online: 5 June 2023 (16:43:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0310.v1

Aerobic Exercise Training Improves Calcium Handling and Cardiac Function in Rats with Heart Failure Resulting from Aortic Stenosis

Vitor Loureiro Da Silva, Gustavo Augusto Ferreira Mota, Sérgio Luiz Borges De Souza, Dijon Henrique Salomé De Campos, Alexandre Barroso Melo, Danielle Fernandes Vileigas, Priscila Murucci Coelho, Paula Grippa Sant’Ana, Carlos Padovani, Ana Paula Lima-Leopoldo, Silméia Garcia Zanati Bazan, Andre Soares Leopoldo, Antonio Carlos Cicogna

Subject: Biology And Life Sciences, Life Sciences Keywords: exercise training; aortic stenosis; heart failure; isolated papillary muscle; isolated cardiomyocyte; calcium handling.

Online: 5 June 2023 (10:47:28 CEST)

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Aerobic exercise training (AET) has been used in the management of heart disease. AET may, totally or partially, restore the activity and/or expression of proteins that regulate calcium (Ca2+) handling, optimize intracellular Ca2+ flow, and attenuate cardiac functional impairment in failing hearts. However, the literature presents conflicting data regarding the effects of AET on Ca2+ transit and cardiac function in rats with heart failure resulting from aortic stenosis. The objective of this study was to evaluate the effects of AET on calcium handling and cardiac function in rats with heart failure due to aortic stenosis. Wistar rats were distributed into two groups: control (Sham; n= 61) and aortic stenosis (AoS; n= 44). After 18 weeks, the groups were redistributed into: non-exposed to exercise training (Sham, n = 28 and AoS, n = 22) and trained (Sham-ET, n = 33 and AoS-ET, n = 22) for 10 weeks. Treadmill exercise training was performed with a velocity equivalent to the lactate threshold. Echocardiogram, isolated papillary muscle, and isolated cardiomyocyte analyzed cardiac function. During isolated papillary muscle assay and isolated cardimyocyte was evaluated Ca2+. The expression of regulatory proteins of diastolic Ca2+ was analyzed via Western Blot. AET attenuated the diastolic dysfunction and benefited the systolic function. AoS-ET animals presented better response to post-rest contraction, and SERCA2a and L-type Ca2+ channels blocked than the AoS. Furthermore, AET was able to improve aspects of the mechanical function and the responsiveness of the myofilaments to the Ca2+ of the AoS-ET animals. AoS animals presented alteration in the protein expression of the SERCA2a and NCX, and AET restored SERCA2a and NCX levels near normal values. Therefore, AET increased SERCA2a activity, improved the cellular Ca2+ influx mechanism, and increased myofilament responsiveness to Ca2+, attenuating cardiac dysfunction at cellular, tissue, and chamber levels in animals with aortic stenosis and heart failure.

Preprint BRIEF REPORT | doi:10.20944/preprints202306.0164.v1

A Non-electrolysis Bioelectric Effect for Gingivitis and Hygiene Contamination Biofilm Removal

Young Wook Kim, Jihyun Lee, Sang Kuy Han, Bon-Sang Koo, Taeguen Park, Hyun Mok Park, Byoungdoo Lee

Subject: Biology And Life Sciences, Life Sciences Keywords: biofilms; bioelectric effect; electrolysis; plaque index; gingival index

Online: 2 June 2023 (09:48:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0031.v1

Detection of a Hypercoagulable State in Patients With Pancreatic Cancer Using a Clot Waveform Analysis

Mayu Kobayashi, Hideo Wada, Shunsuke Fukui, Yasuaki Shimada, Yuuichi Nakazawa, Hiroki Mizutani, Yuhuko Ichikawa, Yuuki Nishiura, Isao Moritani, Yutaka Yamanaka, Hidekazu Inoue, Motomu Shimaoka, Hideto Shimpo, Katsuya Shiraki

Subject: Biology And Life Sciences, Life Sciences Keywords: cancer; pancreatic cancer; CWA; APTT; sTF/FIXa; thrombin time

Online: 1 June 2023 (04:53:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0009.v1

Expression Diﬀerences Between the Eutopic Endometrium With and Without Endometriosis and the Ectopic Endome-trium. A Re-Analysis of Arrays

Muhammad A Riaz, Ezekiel O Mecha, Charles O. A. Omwandho, Felix Zeppernick, Ivo Meinhold-Heerlein, Lutz Konrad

Subject: Biology And Life Sciences, Life Sciences Keywords: endometrium; endometriosis; epithelial-mesenchymal transition; EMT; claudins; keratins

Online: 1 June 2023 (03:10:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2231.v1

Interactive Effects of Ceftriaxone and Chitosan Immobilization on the Production of Arachidonic Acid by and the Microbiome of the Chlorophyte Lobosphaera sp. IPPAS C-2047

Svetlana Vasilieva, Alexandr Lukyanov, Christina Antipova, Timofei Grigoriev, Elena Lobakova, Olga Chivkunova, Pavel Scherbakov, Petr Zaytsev, Olga Gorelova, Tatiana Fedorenko, Dmitry Kochkin, Alexei Solovchenko

Subject: Biology And Life Sciences, Life Sciences Keywords: Lobosphaera; attached cultivation; antibiotics; bioremoval, arachidonic acid

Online: 31 May 2023 (11:00:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2031.v1

Resistance and Biofilm Production Profile of Potential Pathogens Isolated from Kpètè Kpètè Used to Produce Traditional Fermented Beer in Benin

Christine N'Tcha, Haziz Sina, Dyana Ndiade Bourobou, Ismaël M. S. Hoteyi, Bawa Boya, Raul Agnimonhan, Jacques François Mavoungou, Adolphe Adjanohoun, Olubukola Oluranti Babalola, Lamine Baba-Moussa

Subject: Biology And Life Sciences, Life Sciences Keywords: Kpètè Kpètè; microbiological contaminant; Enterobacteria; resistance genes; Staphylococcus spp

Online: 30 May 2023 (04:02:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2008.v1

Radical-Driven Methane Formation in Humans Evidenced by Exogenous Isotope-Labeled DMSO and Methionine

Frank Keppler, Mihály Boros, Daniela Polag

Subject: Biology And Life Sciences, Life Sciences Keywords: endogenously produced methane; reactive oxygen species; methyl radicals; dimethyl sulfoxide; methyl donors; stable carbon and hydrogen isotope labelling; oxidative stress; antioxidant defense system

Online: 29 May 2023 (10:27:42 CEST)

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Methane (CH4), produced endogenously in animals and plants, has recently been suggested to play a role in cellular physiology, potentially influencing the signaling pathways and regulatory mechanisms involved in nitrosative and oxidative stress responses. In addition, it has been proposed that supplementation of CH4 to organisms may be beneficial for the treatment of several diseases, including ischemia, reperfusion injury, and inflammation. However, it is still unclear if and how CH4 is produced in mammalian cells without the help of microorganisms, and how CH4 might be involved in physiological processes in humans. In this study, we provide first proof of the principle that CH4 is formed non-microbially in the human body by applying isotopically labeled methylated sulfur compounds, such as dimethyl sulfoxide (DMSO) and methionine, as carbon precursors to confirm cellular CH4 formation. A volunteer applied isotopically labeled (2H and 13C) DMSO on the skin, orally, and to blood samples. Monitoring of stable isotope values of CH4 convincingly showed the conversion of the methyl groups, as isotopically labeled CH4 was formed during all experiments. Based on these results, we consider several hypotheses about endogenously formed CH4 in humans, including physiological aspects and stress responses involving reactive oxygen species (ROS). While further and broader validation studies are needed, the results may unambiguously serve as a proof of concept for the endoge-nous formation of CH4 in humans by a radical-driven process. Furthermore, these results might encourage follow up studies to decipher the potential physiological role of CH4 and its bioactiv-ity in humans in more detail. Of particular importance is the potential to monitor CH4 as an ox-idative stress biomarker if the observed large variability of CH4 in breath air is an indicator for physiological stress responses and immune reactions. Finally, the potential role of DMSO as a radical scavenger to counteract oxidative stress caused by ROS might be considered in the health sciences. DMSO has already been investigated for many years, but its potential positive role for medical use remained highly uncertain.

Preprint ARTICLE | doi:10.20944/preprints202305.1964.v1

Rapid Prototyping of 3D Printed AgNPs and Nano-TiO₂ Embedded Hydrogels as Novel Devices with Multi-Responsive Antimicrobial Capability in Wound Healing

Giulia Remaggi, Laura Bergamonti, Claudia Graiff, Maria Cristina Ossiprandi, Lisa Elviri

Subject: Biology And Life Sciences, Life Sciences Keywords: 3D printing; silver nanoparticles; titanium dioxide; hydrogels; antimicrobial activity

Online: 29 May 2023 (04:41:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1881.v1

Antiproliferative Activity of Euphorbia ingens Extract against Prostate Cancer Cell Line: An in silico and in vitro Analysis

Innocent Oluwaseun Okpako, Florence Atieno Ng'ong'a, Cleophas Mutinda Kyama, John Chukuwma Oyem, Sospeter Ngoci Njeru

Subject: Biology And Life Sciences, Life Sciences Keywords: Euphorbia ingens; Anticancer; Antiproliferative; Prostate Cancer; Network Pharmacology; Phyto-chemicals; Mechanism of action; Molecular targets

Online: 26 May 2023 (07:46:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1819.v1

Omic Studies on the Effect of Fucus vesiculosus on Differentiated Caco-2 Cells: A Potential Functional Food

Rebeca André, Rita Pacheco, Hugo M. Santos, Maria-Luísa Serralheiro

Subject: Biology And Life Sciences, Life Sciences Keywords: Fucus vesiculosus; proteomics; metabolomics; NPC1; Caco-2 cell line

Online: 26 May 2023 (02:46:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1708.v1

Cytoplasmic Membrane Homeostasis is Important for the Pathogenicity of Pathogenic Fungi

Yuejin Peng, Bin Chen

Subject: Biology And Life Sciences, Life Sciences Keywords: Cytoplasmic membrane homeostasis; Pathogenicity; Pathogenic fungi; Membrane lipid metabolism; Membrane curvature; Transmembrane protein; Cell wall components; Cytoskeleton; Growth and development; Host infection

Online: 24 May 2023 (10:38:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1698.v1

Comparison of Deeply Versus Mildly Invasive Mesothelioma Proteomes Identifies Potential Links Between Mitochondrial Acadvl Expression and Biomarkers of Aggressiveness

Daniel L. Pouliquen, Joanna Kopecka, Stéphanie Blandin, Alice Boissard, Cécile Henry, Chiara Riganti, Catherine Guette

Subject: Biology And Life Sciences, Life Sciences Keywords: Cancer; Aggressiveness; Mitochondria; Acadvl; Fatty acid beta oxidation; Biomarker; Proteomics; Malignant mesothelioma

Online: 24 May 2023 (05:06:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1642.v1

Structural Characterization and Anticancer Activity of a New Anthraquinone from Senna velutina

David Tsuyoshi Hiramatsu Castro, Daniel Ferreira Leite, Debora da Silva Baldívia, Helder Freitas Dos Santos, Sikiru Olaitan Balogun, Denise Brentan Silva, Carlos Alexandre Carollo, Kely De Picoli Souza, Edson Lucas dos Santos

Subject: Biology And Life Sciences, Life Sciences Keywords: melanoma 1; keyword 2; leukemia 3; medicinal plants 4; anthracene 5; caspase 6

Online: 23 May 2023 (10:44:52 CEST)

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Cancer is a complex disease, considered a major public health problem worldwide. Among the types of cancer, melanoma, and leukemias present high mortality rates in Brazil and worldwide. Currently, conventional cytotoxic treatments cause severe side effects by the non-selectivity between normal cells and cancer cells. Therefore, molecules of natural origin with more efficient anticancer properties and that present fewer adverse effects are of extreme importance for cancer therapy and improvement in patients’ quality of life. This study isolated, identified, and characterized the chemical structure of a new anthraquinone present in the extract of the roots of Senna velutina. In addition, we sought to evaluate the anticancer potential of this molecule against melanoma and leukemic cell lines and identify the pathways of cell death involved. To this end, a novel anthraquinone was isolated from the barks of the roots of S. velutina, analyzed by HPLC-DAD, and its molecular structure was determined by NMR. Subsequently, their cytotoxic activity was evaluated by the MTT method against non-cancerous, melanoma, and leukemic cells. The migration of melanoma cells was evaluated by the scratch assay. By flow cytometry technique, the apoptosis process, caspase-3 activation, analysis of mitochondrial membrane potential, and measurement of ROS were evaluated. In addition, the pharmacological cell death inhibitors NEC-1, RIP-1, BAPTA, Z-VAD, and Z-DEVD were used to confirm the related cell death mechanisms. With the results, it was possible to elucidate the novel compound characterized as 2'-OH-Torosaol I. In normal cells, the compound showed no cytotoxicity in PBMC but reduced the cell viability of all melanoma and leukemic cell lines evaluated. 2'-OH-Torosaol I inhibited chemotaxis of B16F10-Nex2, SK-Mel-19, SK-Mel28 and SK-Mel-103. The cytotoxicity of the compound was induced by apoptosis via the intrinsic pathway with reduced mitochondrial membrane potential, increased levels of reactive oxygen species, and activation of caspase-3. In addition, the inhibitors demonstrated the involvement of necroptosis and CA+ in the death process and confirmed caspase-dependent apoptosis death as one of the main programmed-cell death pathways induced by 2'-OH-Torosaol I. Taken together, the data characterize the novel anthraquinone 2'-OH-Torosaol I, demonstrating its anticancer activity and potential application in cancer therapy.

Preprint ARTICLE | doi:10.20944/preprints202305.1613.v1

A Comparative Evaluation of Antioxidant Activity of Extract and Essential Oil of Origanum onites L. In Vivo

Asta Kubiliene, Edvinas Munius, Gabriele Songailaite, Indre Kokyte, Juste Baranauskaite, Arunas Liekis, Ilona Sadauskiene

Subject: Biology And Life Sciences, Life Sciences Keywords: Origanum onites L.; natural products; liposomes; antioxidant activity; mice

Online: 23 May 2023 (08:09:49 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1357.v1

TRP Channels in Tumoral Processes Mediated by Oxidative Stress and Inflammation: Friends or Foes?

Florentina Piciu, Mihaela Balas, Madalina Andreea Badea, Dana Cucu

Subject: Biology And Life Sciences, Life Sciences Keywords: ROS; TRP; inflammation

Online: 19 May 2023 (02:09:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1351.v1

Açaí (Euterpe oleracea Mart.) Seed Oil Exerts a Cytotoxic Role Over Colorectal Cancer Cells: Insights of Annexin A2 Regulation and Molecular Modeling

Marcos Antonio Custódio Neto Da Silva, Josiane Weber Tessmann, Kátia Regina Assunção Borges, Laís Araújo Souza Wolff, Fernanda D. Botelho, Leandro Alegria Vieira, Jose A Morgado-Diaz, Tanos C. C. França, Maria do Carmo Lacerda Barbosa, Maria do Desterro Soares Brandão Nascimento, Murilo R. Rocha, João Ernesto de Carvalho

Subject: Biology And Life Sciences, Life Sciences Keywords: Euterpe oleracea Mart; Polyphenols; Apoptosis; Autophagy; Annexin A2

Online: 18 May 2023 (14:41:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1304.v1

Molecular Networks of Platinum Drugs and Arsenic Trioxide and Its Interaction With microRNAs in Cancer

Shihori Tanabe, Eger Boonstra, Taehun Hong, Sabina Quader, Ryuichi Ono, Horacio Cabral, Kazuhiko Aoyagi, Hiroshi Yokozaki, Hiroki Sasaki

Subject: Biology And Life Sciences, Life Sciences Keywords: drug resistance; cisplatin; arsenic; tumor microenvironment; microRNA; molecular pathway network

Online: 18 May 2023 (08:41:46 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202305.1298.v1

The Renin-Angiotensin-System in COVID-19: Can Long COVID Be Predicted?

Simone König, Richard Vollenberg, Phil-Robin Tepasse

Subject: Biology And Life Sciences, Life Sciences Keywords: Mass spectrometry; RAS; ACE; ACE2; CPN; bradykinin; hypertension; cardiovascular disease; antihypertensiva; ACE inhibitors; angiotensin receptor blockers; bradykinin; neuropeptide re-porter assay

Online: 18 May 2023 (08:13:10 CEST)

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(1) Background: Co-morbidities such as hypertension and cardiovascular disease are major risk factors for severe COVID-19. The renin-angiotensin-system (RAS) is critically involved in their pathophysiology and is counterbalanced by both angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, and the kallikrein-kinin-system (KKS). Considerable research interest with respect to COVID-19 treatment is, thus, currently directed towards the components of these systems. In an earlier study, we noticed significantly reduced carboxypeptidase N (CPN, KKS member) activity and partially excessive angiotensin-converting enzyme (ACE, RAS member) activity in the sera of both hospitalized (HoP) COVID-19 patients and a sub-group of covalescent patients, while in the majority of the probands recovering from the disease these values had returned to normal. The data had been obtained using bradykinin (BK) as a reporter peptide, which is a target of both CPN and ACE, and they were supplemented by serum proteomics of the same patient cohort. We hypothesized that the data could be indicative of Long COVID, which had not been fully appreciated at the time of our study.; (2) Methods: The data were re-evaluated in the light of Long COVID. The recent literature on the RAS in COVID-19, antihypertensiva, and Long COVID was briefly reviewed.; (3) Results: While the levels of the BK serum degradation products should return to normal concentrations during convalescence, this was not true for some patients. This could be due to persisting liver problems, because CPN is synthesized there, but also to a dysregulated RAS. This was not reflected in the levels of selected RAS/KKS serum proteins like angiotensinogen (AGT), although AGT correlated with disease severity in HoP. However, standard tests in routine patient care in Long COVID often come back normal, and it may be that BK degradation is specific in some pathophysiologies. Moreover, the HoP group was sub-divided based on the serum protein profiles and COVID-19 severity.; (4) Conclusions: We point out two insights: 1) Sensitive technology such as omics methods might provide unexpected significant differences within the pre-defined patient groups of a clinical study. Those can only be explored, if the cohorts are large enough and properly matched with respect to the parameters known beforehand (e.g., age, gender, co-morbidities). 2) Results of the BK-reporter serum protease activity assay could be indicative of persisting liver problems and/or potentially of Long COVID. Clinical studies are required to test this hypothesis.

Preprint REVIEW | doi:10.20944/preprints202305.1293.v1

Recent Developments in High Spatio-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications

Wenfa Ng

Subject: Biology And Life Sciences, Life Sciences Keywords: spatial proteomics; spatial resolution; temporal resolution; super-resolution microscopy; fluorescence microscopy,

Online: 18 May 2023 (07:54:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1258.v1

Genetic. Phenotypic and Clinical Heterogeneity of NPM1-Mutant Acute Myeloid Leukemias

Ugo Testa, Elvira Pelosi, Germana Castelli

Subject: Biology And Life Sciences, Life Sciences Keywords: acute myeloid leukemia; genetic classification; mutational profiling; transcriptome analysis; prognostic stratification; clonal evolution

Online: 17 May 2023 (14:36:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1124.v1

Application of Ligand- and Structure-Based Prediction Models for the Design of Alkylhydrazide-Based HDAC3 Inhibitors as Novel Anti-cancer Compounds

Emre f Bülbül, Dina Robaa, Ping Sun, Fereshteh Mahmoudi, Jelena Melesina, Matthes Zessin, Mike Schutkowski, Wolfgang Sippl

Subject: Biology And Life Sciences, Life Sciences Keywords: docking; binding free energy; pharmacophore; KPLS; QSAR; alkylhydrazide; histone deacetylases (HDAC)

Online: 16 May 2023 (08:26:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1106.v1

Trends in Photothermal Nanostructures for Antimicrobial Applications

Violeta Dediu, Jana Ghitman, Gratiela Gradisteanu-Pircalabioru, Kiat Hwa Chan, Florina Silvia Iliescu, Ciprian Iliescu

Subject: Biology And Life Sciences, Life Sciences Keywords: photothermal-antimicrobials; antibacterial mechanisms; anti-biofilm; wound healing

Online: 16 May 2023 (05:40:58 CEST)

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Rapid developing antimicrobial resistance due to broad antibiotic utilisation in healthcare and food industries and the non-availability of novel antibiotics represents one of the most critical public health issues worldwide. The current advances in nanotechnology allow new materials to address drug-resistant bacterial infections in specific, focused and biologically safe ways. The unique physicochemical properties, biocompatibility, and wide range of adaptability of nanomaterials that exhibit photothermal capability can be employed to develop the next generation of photothermally induced controllable hyperthermia as antibacterial nanoplatforms. Here, we review the current state-of-the-art in different functional classes of photothermal antibacterial nanomaterials and the strategies to optimise antimicrobial efficiency. The recent achievements and trends in developing photothermally active nanostructures, including plasmonic metals, semiconductor, carbon-based, and organic photothermal polymers, and antibacterial mechanisms of action, including anti-multidrug resistant bacteria and biofilms removal, will be discussed. Insights into mechanisms of the photothermal effect and various factors influencing photothermal antimicrobial performance, emphasising the structure–performance relationship, are discussed. We will examine the photothermal agents’ functionalisation for specific bacteria, effects of near-infrared light irradiation spectrum, or active photothermal materials for multimodal synergistic-based therapies to minimise side effects and maintain low cost. Most relevant applications are presented, such as anti-biofilm formation, biofilm penetration or ablation, and nanomaterial-based infected wound therapy. Practical antibacterial applications employing the photothermal antimicrobial agents, alone or in synergistic combination with other nanomaterials, are considered. Existing challenges and limitations in photothermal antimicrobial therapy and future perspectives are presented from the structure, function, safety, and clinical potential points of view.

Preprint ARTICLE | doi:10.20944/preprints202305.1028.v1

Long-Term Supplementation of Ozonated Sunflower Oil Improves Dyslipidemia and Hepatic Inflammation in Hyperlipidemic Zebrafish (Danio rerio): Suppression of Oxidative Stress and Inflammation Against Carboxymethyllysine Toxicity

Kyung-Hyun Cho, Ji-Eun Kim, Ashutosh Bahuguna, Dae-Jin Kang

Subject: Biology And Life Sciences, Life Sciences Keywords: ozonated sunflower oil, OSO; antioxidant; dyslipidemia; high-cholesterol diet, HCD; zebrafish; zebrafish embryo; carboxymethyllysine, CML

Online: 15 May 2023 (10:42:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0987.v1

Certainty Over Uncertainty: A Search for an Endemic Amphibia in Indo-Burma Hotspot Guided by a Predictive Distribution Modeling Approach

Samrat Sengupta, Subhasish Arandhara, Bhaskar Saikia

Subject: Biology And Life Sciences, Life Sciences Keywords: Maximum Entropy; species distribution model; ground validation; endemic; tree-frog; Protected Area; Biodiversity Hotspot

Online: 15 May 2023 (07:19:47 CEST)

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Conservation of tropical endemic amphibians largely suffers from Wallacean shortfall, a gap to which predictive species distribution models have contributed significantly to bridge by delineating probable distribution and the underlying suitable habitat within their distributional range. However, rarely is a prediction model ground-truthed to evaluate their predictive performance. Here we present a species distribution modeling approach using maximum entropy algorithm corrected for smaller sample size, in guiding explorative surveys aimed at optimizing survey effort and discovering unrecorded populations of Zhangixalus suffry, a rhacophorid tree-frog endemic to the northeastern part of the Indian subcontinent along with the factors limiting their distribution. With only 16 established historical locality data to model for (after spatial thinning to reduce autocorrelation) and a set of environmental predictors (climatic, topographic, and landscape composition), our model prediction enabled the successful discovery of seven new population records from unreported landscapes, extending its southernmost distributional limit over a considerable distance. The final composite distribution model combining all the locality records (n=23) predicted similar core areas of suitable habitat consistent with the known geographic distribution of the species but showed poor representation under existing coverage of Protected Area (PA) network in the Region with only 7% representation of suitable habitat under protection. Habitat suitability of a site was significantly governed by factors related to precipitation (precipitation seasonality and precipitation of the warmest quarter) and topographic factors that can influence it (elevation and aspect). This corroborates with the known ecology of Rhacophorid frogs, especially concerning their seasonal explosive reproductive strategy and foam nest-building behavior.Through this study, we propose explorative surveys guided by species distribution models to expedite unknown population discovery of rare, tropical endemic amphibians and using such taxa as surrogates in identifying conservation priority zones that can be directly applied to reserve design and conservation and management planning.

Preprint REVIEW | doi:10.20944/preprints202305.0893.v1

Long Non-Coding RNAs as Emerging Targets in Lung Cancer

Jovanka Gencel-Augusto, Wei Wu, Trever G. Bivona

Subject: Biology And Life Sciences, Life Sciences Keywords: lncRNAs; non-coding RNAs; lung cancer; metastasis; biomarkers; therapy resistance

Online: 12 May 2023 (05:24:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0881.v1

Livelihoods and Perceptions of Climate Change among Dairy Farmers in the Andes: Implications for Climate Education

Julio C Vargas-Burgos, Marco Heredia-R, Yenny Torres, Laura Elena Puhl, Biviana N Heredia, Jhenny Cayambe, Julio Hernán-González, Alexandra Torres, Marcelo Luna, Theofilos Toulkeridis, Bolier Torres

Subject: Biology And Life Sciences, Life Sciences Keywords: Climate change; livestock farmers; rural livelihoods; climate education.

Online: 12 May 2023 (04:33:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0838.v1

Down-Syndrome Development might be Associated with Dysbiosis, Triggered by Certain Classes of Antibiotics: Altered Maternal Microbiome as a New Insight into the Genomic Events

Ternák Gábor, Gergely Márovics, Katalin Sümegi, Zsolt Bánfai, Gergely Büki, Lili Magyari, András Szabó, Bela Melegh

Subject: Biology And Life Sciences, Life Sciences Keywords: Down syndrome [DS]; microbiome; dysbiosis; antibiotics; tetracycline; penicillin

Online: 11 May 2023 (10:04:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0787.v1

Impact of Climate Change on Phenology of Entomophilous Plants and Honey Bee (Apis mellifera L.)

Danuta Romanovskaja, Eugenija Baksiene, Almantas Razukas

Subject: Biology And Life Sciences, Life Sciences Keywords: entomophilous plants; phenophases; honeybee; climate change

Online: 11 May 2023 (04:47:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0739.v1

Exploring the Development of Astrobiology Scientific Research Through Bibliometric Network Analysis: A Focus on Biomining and Bioleaching

Luca Tonietti, Bernardo Barosa, Emiliano Pioltelli, Donato Giovannelli, Giovanni Covone, Paola Di Donato, Angelina Cordone, Laura Inno, Christian Magliano, Stefano Fiscale, Maria Teresa Muscari Tomajoli, Gaetana Napolitano, Alice Maria Piccirillo, Vincenzo Della Corte, Rosa Santomartino, Alessandra Rotundi

Subject: Biology And Life Sciences, Life Sciences Keywords: biomining; bioleaching; astrobiology; A. ferrooxidans; acidophiles; space biology; bibliometric network analysis

Online: 10 May 2023 (10:38:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0680.v1

Red and Golden Tomato Administration Improves Fat Di-Et-Induced Hepatic Steatosis in Rats by Modulating HNF4, Lepr and GK Expression

Rosaria Maria Pipitone, Rossella Zito, Giuditta Gambino, Gabriele Di Maria, Ayesha Javed, Giulia Lupo, Giuseppe Giglia, Pierangelo Sardo, Giuseppe Ferraro, Francesca Rappa, Daniela Carlisi, Danila Di Majo, Stefania Grimaudo

Subject: Biology And Life Sciences, Life Sciences Keywords: Nonalcoholic Fatty Liver Disease; Golden Tomato; Red Tomato; Lepr; NFH4a; GK; lipid homeo-stasis; steatosis

Online: 10 May 2023 (04:01:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0665.v1

Carboxyhemoglobin (COHb): Unavoidable Bystander or Protective Player?

André Carrola, Carlos C. Romão, Helena L. A. Vieira

Subject: Biology And Life Sciences, Life Sciences Keywords: carbon monoxide; hemoglobin; carboxyhemoglobin; glutathione; antioxidant

Online: 9 May 2023 (13:36:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0653.v1

GDF15 promotes the Osteogenic Cell Fate of Periodontal Ligament Fibroblasts affecting their Mechanobiological Response

Lukas Lösch, Albert Stemmler, Adrian Fischer, Julia Steinmetz, Lisa Schuldt, Christoph-Ludwig Hennig, Judit Symmank, Collin Jacobs

Subject: Biology And Life Sciences, Life Sciences Keywords: GDF15; Orthodontic tooth movement; Periodontal ligament fibroblasts; Osteoblast differentiation; Mechanobiological response; Inflammation; Osteoclast Activation

Online: 9 May 2023 (12:33:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0643.v1

Cells and Processes in Wound Healing: More Recent Advances

Montserrat Fernández-Guarino, María Luisa Hernández-Bule, Stefano Bacci

Subject: Biology And Life Sciences, Life Sciences Keywords: Acute Wounds; Cellular Infiltrate; Chronic Wounds; Keloids; Scars

Online: 9 May 2023 (11:06:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0528.v1

Comparative Effects of Botanical Powders in Controlling Sitophilus zeamais (Maize Weevils) in Stored Maize (Zea mays L.)

Ojuolape Damilola Olanrewaju, Jennifer Chinenye Opara, Ofodile Peter Nwite, Usman Umoru, Mukhtar Sidi Aliyu

Subject: Biology And Life Sciences, Life Sciences Keywords: Botanicals; Sitophilus zeamais; Maize; Storage; Synthetic Pesticide; Pest; Efficacy

Online: 8 May 2023 (10:56:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0495.v1

Antioxidative and Anti-inflammatory Activities of Rosebud Extracts of Newly-Crossbred Roses

Cuicui Wang, In-Jeong Kim, Hye-Rim Seong, Chan Ho Noh, Sangryong Park, Tae Myoung Kim, Heon Sang Jeong, Ka Young Kim, Seung Tae Kim, Hyun-Gyun Yuk, Sang-Chul Kwon, Ehn-Kyoung Choi, Yun-Bae Kim

Subject: Biology And Life Sciences, Life Sciences Keywords: rosebud extract; Pretty Velvet; polyphenol; antioxidation; anti-inflammation; nitric oxide; prostaglandin E2

Online: 8 May 2023 (09:37:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0436.v1

Knowledge and Attitudes towards HIV and HCV among the General Population in Brescia, Italy

Francesca Viola, Beatrice Formenti, Stefania Arsuffi, Itala Polesini, Emanuele Focà, Francesco Castelli, Eugenia Quiros-Roldan

Subject: Biology And Life Sciences, Life Sciences Keywords: HIV; HCV; Health Literacy; HIV Knowledge; HCV Knowledge; Viral Infections

Online: 8 May 2023 (03:50:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0422.v1

Novel Bone Void Filling Cement Compositions based on Shell Nacre and Siloxane Methacrylate Resin: Development and Characterization

Bridget Jeyatha W, Lizymol P.P.

Subject: Biology And Life Sciences, Life Sciences Keywords: shell nacre; ormocer; bone void filling cement; bone defects

Online: 6 May 2023 (10:57:53 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202305.0314.v1

Cell-Free Methylated PTGER4 and SHOX2 Plasma DNA as a Biomarker for Therapy Monitoring and Prognosis in Advanced Stage NSCLC Patients

Michael Fleischhacker, Erkan Arslan, Dana Reinicke, Stefan Eisenmann, Gerit Theil, Jens Kollmeier, Christoph Schaeper, Christian Grah, Frank Klawonn, Stefan Holdenrieder, B. Schmidt

Subject: Biology And Life Sciences, Life Sciences Keywords: liquid profiling; plasma; methylation; mSHOX2; mPTGER4

Online: 5 May 2023 (07:23:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202212.0272.v2

Apiaceae Medicinal Plants: A Review of Traditional Uses, Phytochemistry, Bolting and Flowering, and Controlling Approaches

Meiling Li, Min Li, Li Wang, Mengfei Li, Jianhe Wei

Subject: Biology And Life Sciences, Life Sciences Keywords: Apiaceae plants; traditional use; phytochemistry; bolting and flowering; controlling approaches

Online: 5 May 2023 (05:47:46 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0223.v1

The Luminous Fungi of Japan

Yuichi Oba, Kentaro Hosaka

Subject: Biology And Life Sciences, Life Sciences Keywords: bioluminescence; fungi; mushroom; Japan

Online: 4 May 2023 (08:01:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0203.v1

Ubiquitin Degradation of the AICAR Transformylase/IMP Cy-Clohydrolase Ade16 Regulates the Sexual Reproduction of Cryptococcus neoformans

Lian-Tao Han, Yu-Juan Wu, Si-Chu Xiong, Tong-Bao Liu

Subject: Biology And Life Sciences, Life Sciences Keywords: Cryptococcus neoformans; Sexual reproduction; F-box protein Fbp1; AICAR transformylase/IMP cyclohydrolase, Ade16

Online: 4 May 2023 (05:46:43 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0188.v1

Cross-Sectional Use of Vascular, Blood-Based Biomarkers—Augmenting Postmortem

Ivam Šoša

Subject: Biology And Life Sciences, Life Sciences Keywords: blood-based biomarkers; clotting; inflammation; postmortem; vascular biomarkers

Online: 4 May 2023 (04:25:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0152.v1

Temperature-Humidity Dependent Wind Effects on Physiological Heat Strain of Moderately Exercising Individuals Reproduced by the Universal Thermal Climate Index UTCI

Peter Bröde, Bernhard Kampmann

Subject: Biology And Life Sciences, Life Sciences Keywords: heat stress; air temperature; humidity; wind; heat wave; electrical fan; index

Online: 3 May 2023 (12:52:02 CEST)

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Increasing wind speed alleviates physiological heat strain, however, health policies have advised against using ventilators or fans under heat wave conditions with air temperatures above the typical skin temperature of 35 °C. Recent research, mostly with sedentary participants, suggests mitigating effects of wind at even higher temperatures depending on the humidity level. Our study aimed at exploring and quantifying, whether such results are transferable to moderate exercise levels, and whether the Universal Thermal Climate Index UTCI reproduces those effects. We measured heart rates, core and skin temperatures and sweat rates in 198 laboratory experiments completed by five young, semi-nude, heat-acclimated, moderately exercising males walking the treadmill with 4 km/h on the level for three hours under widely varying temperature-humidity combinations and two wind conditions. We quantified the cooling effect of increasing wind speed from 0.3 to 2 m/s by fitting generalized additive models predicting the physiological heat stress responses depending on ambient temperature, humidity and wind speed. We then compared the observed wind effects to the assessment performed by UTCI. Increasing wind speed lowered physiological heat strain for air temperatures below 35 °C, but also for higher temperatures with humidity levels above 2 kPa water vapour pressure concerning heart rate and core temperature, and 3 kPa concerning skin temperature and sweat rate, respectively. UTCI assessment of wind effects correlated positively with the observed changes in physiological responses, showing the closest agreement (r=0.9) for skin temperature and sweat rate, where wind is known for elevating the relevant convective and evaporative heat transfer. These results demonstrate the potential of UTCI for adequately assessing sustainable strategies for heat stress mitigation involving fans or ventilators depending on temperature and humidity for moderately exercising individuals.

Preprint ARTICLE | doi:10.20944/preprints202304.1257.v1

Evidence of Biparental Mitochondrial Inheritance from Self-Fertile Crosses between Closely Related Species of Ceratocystis

Daniella Van der Walt, Emma Steenkamp, Brenda D. Wingfield, P. Markus Wilken

Subject: Biology And Life Sciences, Life Sciences Keywords: Ceratocystis; hybridization; PCR RFLP

Online: 30 April 2023 (04:34:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1207.v1

Malaria Risk Drivers in the Brazilian Amazon: Land Use: Land Cover Interactions and Biological Diversity

William Gonzalez-Daza, Renata Muylaert, Thadeu Sobral-Souza, Victor Lemes-Landeiro

Subject: Biology And Life Sciences, Life Sciences Keywords: Malaria; Amazon biome; INLA; Land use/cover interactions; Bird and amphibian rich-ness-endemics; Landscape composition; Biological diversity; Spatio-temporal modeling

Online: 29 April 2023 (04:29:50 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202304.1121.v1

West African Goliath Groupers: Where are they between Senegal and Angola?

João Pedro Barreiros

Subject: Biology And Life Sciences, Life Sciences Keywords: Atlantic Goliath Grouper; Epinephelus itajara; extinction; overfishing; marine protected areas; monitoring

Online: 28 April 2023 (05:18:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1073.v1

Comparative Analysis of Cell Mixtures Deconvolution and Gene Signatures Generated for Blood, Immune and Cancer Cells

Natalia Alonso-Moreda, Alberto Berral-Gonzalez, Enrique De La Rosa, Oscar González-Velasco, Jose Manuel Sanchez-Santos, Javier De Las Rivas

Subject: Biology And Life Sciences, Life Sciences Keywords: cell mixture; deconvolution; immune cells; blood cells; cancer cells; gene signature; bioinformatics

Online: 27 April 2023 (10:34:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1067.v1

Designing a Multi-Epitope Subunit Vaccine against VP1 Major Coat Protein of JC Polyomavirus

Sukhada Kanse, Mehak Khandelwal, Rajan Kumar Pandey, Manoj Khokhar, Neetin S Desai, Bajarang Kumbhar

Subject: Biology And Life Sciences, Life Sciences Keywords: Progressive Multifocal Encephalopathy (PML); JC Polyomavirus; VP1 protein; Immunoinformatics; molecular dynamics simulation

Online: 27 April 2023 (10:02:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1057.v1

Genetic Polymorphisms of ACE1 rs4646994 Associated with Lung Cancer in Patients with Pulmonary Nodules: A Case Control Study

Rong Qiao, Siyao Sang, Jiajun Teng, Hua Zhong, Hui Li, Baohui Han

Subject: Biology And Life Sciences, Life Sciences Keywords: genetic polymorphism; ACE1 rs4646994; lung cancer; pulmonary nodules

Online: 27 April 2023 (07:47:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1028.v1

Evaluation of DNA Methylation Profiles of LINE-1, Alu and Ribosomal DNA Repeats in Human Cell Lines Exposed to Radiofrequency Radiation

Francesco Ravaioli, Maria Giulia Bacalini, Cristina Giuliani, Camilla Pellegrini, Chiara D'Silva, Sara De Fanti, Chiara Pirazzini, Gianfranco Giorgi, Brunella Del Re

Subject: Biology And Life Sciences, Life Sciences Keywords: Radiofrequency electromagnetic field; DNA methylation; Neuroblastoma; LINE-1; Alu; ribosomal DNA

Online: 27 April 2023 (04:12:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0855.v1

Global Profile of tRNA-Derived Small RNAs in Pathological Cardiac Hypertrophy Plasma and Identification of tRF-21-NB8PLML3E as a New Hypertrophy Suppressor

Jingyi Xu, Buyun Qian, Feng Wang, Ying Huang, Xinxin Yan, Ping Li, Qian Zhang, Yuan Li, Kangyun Sun

Subject: Biology And Life Sciences, Life Sciences Keywords: transfer RNA-derived small RNAs; pathological cardiac hypertrophy; small RNA sequencing; biomarkers

Online: 24 April 2023 (11:01:16 CEST)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202304.0815.v1

Countering Biothreats from Low-Effort Toxins: Some Highlights from the Chemical and Biological Investigation, Evidence, and Countermeasures Training

Subhash Janardhan Bhore

Subject: Biology And Life Sciences, Life Sciences Keywords: biosafety; biorisk; biosecurity; biosecurity engagement program; bioterrorism; CBRNE; cooperative threat reduction; homemade explosives; southeast Asia; sustainability; toxins

Online: 24 April 2023 (03:57:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0797.v1

Nutritional Supplementation and Exercise – Essential Allies in the Treatment of Chronic Heart Failure: The Metabolic and Molecular Bases.

Evasio Pasini, Giovanni Corsetti, Francesco Saverio Dioguardi

Subject: Biology And Life Sciences, Life Sciences Keywords: Heart failure; Malnutrition; Exercise; Amino-acids; mTOR; Deptor

Online: 23 April 2023 (08:45:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0478.v2

Mutant IDH in Gliomas: Role in Cancer and Treatment Options

Georgios Solomou, Alina Finch, Asim Asghar, Chiara Bardella

Subject: Biology And Life Sciences, Life Sciences Keywords: isocitrate dehydrogenase; cancer metabolism; hydroxyglutarate; gliomas; oncometabolites

Online: 20 April 2023 (02:28:47 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0538.v1

The Roles of Ral-Interacting Protein 76 and Vascular Endothelial Growth Factor in the Ovarian Corpus Luteum and Tumor Microenvironment

Tae-Hee Min, Seunghyung Lee

Subject: Biology And Life Sciences, Life Sciences Keywords: Ral-interacting protein 76; vascular endothelial growth factor; small GTPase; hypoxia-inducible factor-1; corpus luteum; tumor

Online: 19 April 2023 (04:10:47 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0478.v1

Mutant IDH in Gliomas: Role in Cancer and Treatment Options

Georgios Solomou, Alina Finch, Asim Asghar, Chiara Bardella

Subject: Biology And Life Sciences, Life Sciences Keywords: isocitrate dehydrogenase, cancer metabolism, hydroxyglutarate, gliomas, oncometabolites

Online: 18 April 2023 (04:12:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0433.v1

Super-Resolution Ultrasound Localization Microscopy using High-Frequency Ultrasound to Measure Ocular Perfusion Velocity in the Rat Eye

Hasan Ul Banna, Benjamin Mitchell, Stephen Chen, Joel Palko

Subject: Biology And Life Sciences, Life Sciences Keywords: glaucoma; intraocular pressure; ocular hemodynamics; ocular vasculature; super- 19 resolution ultrasound; ultrasound localization microscopy

Online: 17 April 2023 (09:40:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0414.v1

Potential Benefits of Omega-3 Polyunsaturated Fatty Acids (N3PUFAs) on Cardiovascular Health Associated with COVID-19: An Update for 2023

Louise Weiwei Lu, Siew-Young Quek, Shiping Lu, Jie-Hua Chen

Subject: Biology And Life Sciences, Life Sciences Keywords: N3PUFA; EPA; DHA; omega-3; cardiovascular; coagulation; thrombosis; COVID-19; inflammation; oxidative stress.

Online: 17 April 2023 (07:01:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0388.v1

Association of TP53 Single Nucleotide Polymorphisms with Prostate Cancer in a Racially Diverse Cohort of Men

Allison Duncan, Darryl Nousome, Randy Ricks, Huai-Ching Kuo, Lakshmi Ravindranath, Albert Dobi, Jennifer Cullen, Shiv Srivastava, Gregory Chesnut, Gyorgy Petrovics, Indu Kohaar

Subject: Biology And Life Sciences, Life Sciences Keywords: prostate cancer; SNP; TP53; Pro47Ser; Arg72Pro; African American

Online: 17 April 2023 (03:41:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0335.v1

Feasibility and Efficacy of Morning Light Therapy for Adults with Sleep Disorders: A Pilot Randomized Open Label Two-Arm Study

Jihyun Yoon, Seok-Jae Heo, Hyangkyu Lee, Eun Gyeong Sul, Tae-Hwa Han, Yu-Jin Kwon

Subject: Biology And Life Sciences, Life Sciences Keywords: light therapy; sleep disorder; daytime sleepiness; circadian rhythm; clock gene

Online: 14 April 2023 (03:14:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0231.v1

The Life Sequence Conjecture and the Theory of the Reptoid

Ozzy K. King

Subject: Biology And Life Sciences, Life Sciences Keywords: reptilian; reptoid; ufology; Life Sequence Conjecture; extraterrestrials; convergent evolution; homologous evolution; parallel evolution; divergent evolution; chaos evolution

Online: 12 April 2023 (03:09:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0221.v1

The Genome of Varunaivibrio sulfuroxidans Strain TC8^T, a Metabolically Versatile Alphaproteobacterium from the Tor Caldara Gas Vents in the Tyrrhenian Sea

Sushmita Patwardhan, Jonathan Phan, Francesco Smedile, Costantino Vetriani

Subject: Biology And Life Sciences, Life Sciences Keywords: shallow-water vent; Tyrrhenian Sea; Tor Caldara; chemolithotroph; Alphaproteobacteria; Thalasso-spiraceae; genome; geothermal; sulfur oxidation; DNA uptake; nitrogen fixation; carbon fixation

Online: 11 April 2023 (10:31:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0160.v1

Biophysical Interactions Underpin the Emergence of Information in the Genetic Code

Aaron Halpern, Lilly R Bartsch, Kaan Ibrahim, Stuart A Harrison, Minkoo Ahn, John Christodoulou, Nick Lane

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; genetic code; biophysical interactions; hydrophobicity; anticodon; molecular dynamics; NMR

Online: 10 April 2023 (09:07:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0125.v1

De Novo Venom Gland Transcriptome Assembly and Characterization for Calloselasma rhodostoma (Kuhl, 1824), the Malayan Pit Viper from Malaysia: Unravelling Toxin Gene Diversity in a Medically Important Basal Crotaline

Choo Hock Tan, Kae Yi Tan, Tzu Shan Ng, Nget Hong Tan, Ho Phin Chong

Subject: Biology And Life Sciences, Life Sciences Keywords: kistomin; rhodostoxin; rhodostomin; rhodocytin; rhodocetin; ancrod

Online: 7 April 2023 (11:36:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0110.v1

The Informational Model of the Human Body and Living Structures: from Micro to Macro Structuration and Functions

Florin Gaiseanu

Subject: Biology And Life Sciences, Life Sciences Keywords: Information; matter-related information; micro/macro structuration/destructuration; non-living/living structures; Informational Model of the Human Body and Living Structures; Infor-mational Model of Consciousness

Online: 7 April 2023 (03:56:25 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202304.0068.v1

Preliminary Data : Feeding and Colon Cancer Growth. Modified Nitrogen Intake Leads to Blunted Cancer Growth Independently by Serine

Giovanni Corsetti, Claudia Romano, Tiziano Scarabelli, Carol Chen-Scarabelli, Evasio Pasini, And Francesco S Dioguardi

Subject: Biology And Life Sciences, Life Sciences Keywords: cancer; amino acids; colon; nitrogen; diet; mice

Online: 5 April 2023 (15:45:18 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202304.0041.v1

Identification of Novel Biomarkers in Peripheral Blood Mononuclear Cells of Hepatocellular Carcinoma Patients

Sindhoora R

Subject: Biology And Life Sciences, Life Sciences Keywords: Hepatology; Oncology; Biomarkers; Hepatocellular Carcinoma

Online: 4 April 2023 (09:28:52 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202304.0037.v1

Intrahepatic Cholangiocarcinoma and Acute Intermittent Porphyria: A Case Report

Claudio Carmine Guida, Maria Nardella, Leonardo Fiorentino, Tiziana Latiano, Francesco Napolitano, Gaetano Ferrara, Annalisa Crisetti, Gianluigi Mazzoccoli, Francesco Aucella, Filippo Aucella

Subject: Biology And Life Sciences, Life Sciences Keywords: acute hepatic porphyria; cholangiocarcinoma; delta aminolevulinic acid; porphobilinogen; hydroxymethylbilane synthase

Online: 4 April 2023 (04:53:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202303.0448.v1

On the Roles of the Nuclear Non-coding RNA-Dependent Membrane-Less Organelles in the Cellular Stress Response

Anastasia A. Gavrilova, Anna S. Fefilova, Irina M. Kuznetsova, Konstantin K. Turoverov, Vladimir N. Uversky, Alexander V. Fonin

Subject: Biology And Life Sciences, Life Sciences Keywords: LLPS; liquid-liquid phase separation; non-coding RNA; intrinsically disordered proteins; membrane-less organelles; stress-response; biomolecular condensates

Online: 27 March 2023 (05:43:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202206.0370.v1

How Did Life Emerge in Chemically Complex Messy Environments?

Kenji Ikehara

Subject: Biology And Life Sciences, Life Sciences Keywords: GADV hypothesis; origin of life; protein 0th-order structure; origin of protein; [GADV]-microsphere; origin of gene: the core life system

Online: 28 June 2022 (03:41:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0287.v1

The Dissipative Photochemical Origin of Life: UVC Abiogenisis of the Purines

Claudeth Clarissa Hernández, Karo Michaelian

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; disspative structuring; non-equilibrium thermodynamics; prebiotic chemistry; abiogenisis; adenine; guanine; hypoxanthine; xanthine; purines

Online: 21 June 2022 (05:14:12 CEST)

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Preprint REVIEW | doi:10.20944/preprints202202.0031.v1

Origin and Early Evolution of Photosynthesis: A Brief Historical Account

Tanai Cardona

Subject: Biology And Life Sciences, Life Sciences Keywords: photosynthesis; photosystem; water oxidation; oxygenic; evolution; origin of life

Online: 2 February 2022 (12:17:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202101.0500.v1

The Dissipative Photochemical Origin of Life: UVC Abiogenesis of Adenine

Karo Michaelian

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; disspative structuring; prebiotic chemistry; abiogenisis; adenine

Online: 25 January 2021 (13:57:05 CET)

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Preprint ARTICLE | doi:10.20944/preprints202009.0409.v1

Did Biology Emerge from Biotite in Micaceous Clay?

H. Greenwood Hansma

Subject: Biology And Life Sciences, Life Sciences Keywords: clay; mica; biotite; muscovite; origin of life; origins of life; mechanical energy; work; wet-dry cycles

Online: 17 September 2020 (13:01:15 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints201911.0193.v1

Human Origins in Southern African Palaeo-wetlands? Strong Claims from Weak Evidence

Carina M. Schlebusch, Liisa Loog, Huw S. Groucutt, Turi King, Adam Rutherford, Chiara Barbieri, Guido Barbujani, Lounès Chikhi, Mattias Jakobsson, Anders Eriksson, Andrea Manica, Sarah A. Tishkoff, Eleanor M.L. Scerri, Aylwyn Scally, Chris Brierley, Mark G. Thomas

Subject: Biology And Life Sciences, Life Sciences Keywords: human origins; mitochondrial DNA; Africa; human evolution

Online: 17 November 2019 (00:55:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints201910.0086.v1

How Codon Usage Bias Affects Our Ability to Recover the Tree of Life

Justin B. Miller, Michael F. Whiting, John S.K. Kauwe, Perry G. Ridge

Subject: Biology And Life Sciences, Life Sciences Keywords: phylogenomics; phylogenetics; codon usage bias; Tree of Life

Online: 8 October 2019 (10:43:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201806.0035.v2

The First Universal Common Ancestor (FUCA) as the Earliest Ancestor of LUCA’s (Last UCA) Lineage

Francisco Prosdocimi, Marco José, Sávio Farias

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; LUCA; FUCA; RNA World; PTC; Archaea; translation system

Online: 23 July 2019 (08:13:32 CEST)

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Working Paper ARTICLE

Mechanical Energy before Chemical Energy at the Origins of Life?

H. Greenwood Hansma

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; origins of life; mechanical energy; work; entropic forces; mica; biotite; Muscovite; wet/dry cycles; clay

Online: 29 April 2019 (07:51:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints201712.0170.v1

Life Emerged from [GADV]-Protein World, but Not from RNA World!?

Kenji Ikehara

Subject: Biology And Life Sciences, Life Sciences Keywords: RNA world; [GADV]-protein world; GADV hypothesis; origin of life; protein 0th-order structure; origin of protein; origin of genetic code; origin of gene

Online: 25 December 2017 (08:08:37 CET)

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Biology and Life Sciences