Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Circadian Rhythm Perturbation Aggravates Gut Microbiota Dysbiosis in Dextran Sulfate Sodium-Induced Colitis in Mice

Joseph Amara
,
Tarek Itani
ORCID logo ,
Joelle Hajal
,
Jules-Joel Bakhos
,
Youakim Saliba
ORCID logo ,
Saied A. Aboushanab
ORCID logo ,
Elena G. Kovaleva
* ,
Nassim Fares
ORCID logo ,
Alicia C. Mondragon
,
Jose Manuel miranda lopez
* ORCID logo
Version 1 : Received: 9 January 2024 / Approved: 11 January 2024 / Online: 11 January 2024 (11:27:26 CET)

A peer-reviewed article of this Preprint also exists.

Amara, J.; Itani, T.; Hajal, J.; Bakhos, J.-J.; Saliba, Y.; Aboushanab, S.A.; Kovaleva, E.G.; Fares, N.; Mondragon, A.C.; Miranda, J.M. Circadian Rhythm Perturbation Aggravates Gut Microbiota Dysbiosis in Dextran Sulfate Sodium-Induced Colitis in Mice. Nutrients 2024, 16, 247. https://doi.org/10.3390/nu16020247 Amara, J.; Itani, T.; Hajal, J.; Bakhos, J.-J.; Saliba, Y.; Aboushanab, S.A.; Kovaleva, E.G.; Fares, N.; Mondragon, A.C.; Miranda, J.M. Circadian Rhythm Perturbation Aggravates Gut Microbiota Dysbiosis in Dextran Sulfate Sodium-Induced Colitis in Mice. Nutrients 2024, 16, 247. https://doi.org/10.3390/nu16020247

Abstract

Circadian rhythm disruption is increasingly considered an environmental risk factor for the development and exacerbation of inflammatory bowel disease. We have reported in a previous study that nychthemeral dysregulation is associated with an increase in intestinal barrier permeability and inflammation in mice with dextran sulfate sodium (DSS)-induced colitis. To investigate the effect of circadian rhythm disruption on the composition and diversity of the gut microbiota (GM). Sixty male C57BL/6J mice were divided initially to two groups, the shifted group (n=30) exposed to circadian shifts for three months and the non-shifted group (n=30) under a normal light-dark cycle. The mice of the shifted group were cyclically housed for five days under the normal 12:12 hour light-dark cycle followed by another five days under reversed light-dark cycle. At the end of the three months a colitis was induced by 2 % DSS given in the drinking water of 30 mice. Animals were then divided into four groups (n=15 per group), sham group non-shifted (Sham-NS), sham group shifted (Sham-S) and DSS non-shifted (DSS-NS) and DSS shifted (DSS-S). Fecal samples were collected from rectal content to investigate changes in GM composition by DNA extraction followed by high-throughput sequencing of the bacterial 16S rRNA gene. The mouse GM was dominated by three phyla: Firmicutes, Bacteroidetes and Actinobacteria. The Firmicutes/Bacteroidetes ratio decreased in mice with induced colitis. The richness and diversity of the GM were reduced in the colitis group, especially in the group with inverted circadian rhythm. Moreover, GM composition was also modified in the inverted circadian rhythm group, with an increase in Alloprevotella, Turicibacter, Bacteroides and Streptococcus genera. Circadian rhythm inversion exacerbates GM dysbiosis to a less rich and diversified extent in a DSS-induced colitis model. These findings shows a possible interplay between circadian rhythm disruption, GM dynamics, and colitis pathogenesis.

Keywords

DSS-induced colitis; Dysbiosis; Circadian rhythm; Bacterial diversity; Microbiota; Gut

Subject

Public Health and Healthcare, Public Health and Health Services

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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