Version 1
: Received: 12 May 2021 / Approved: 13 May 2021 / Online: 13 May 2021 (14:01:21 CEST)
How to cite:
Joshi, T.; Kumar, S.; Arya, L.; Riar, A. Distance Only Brings You Closer: Application of ISSR Markers to Analyze Molecular Relationships in Roses (Rosa spp.) - The Symbol of Love. Preprints2021, 2021050305. https://doi.org/10.20944/preprints202105.0305.v1
Joshi, T.; Kumar, S.; Arya, L.; Riar, A. Distance Only Brings You Closer: Application of ISSR Markers to Analyze Molecular Relationships in Roses (Rosa spp.) - The Symbol of Love. Preprints 2021, 2021050305. https://doi.org/10.20944/preprints202105.0305.v1
Joshi, T.; Kumar, S.; Arya, L.; Riar, A. Distance Only Brings You Closer: Application of ISSR Markers to Analyze Molecular Relationships in Roses (Rosa spp.) - The Symbol of Love. Preprints2021, 2021050305. https://doi.org/10.20944/preprints202105.0305.v1
APA Style
Joshi, T., Kumar, S., Arya, L., & Riar, A. (2021). Distance Only Brings You Closer: Application of ISSR Markers to Analyze Molecular Relationships in Roses (<em>Rosa spp</em>.) - The Symbol of Love. Preprints. https://doi.org/10.20944/preprints202105.0305.v1
Chicago/Turabian Style
Joshi, T., Lalit Arya and Amritbir Riar. 2021 "Distance Only Brings You Closer: Application of ISSR Markers to Analyze Molecular Relationships in Roses (<em>Rosa spp</em>.) - The Symbol of Love" Preprints. https://doi.org/10.20944/preprints202105.0305.v1
Abstract
Genetic diversity is inevitable in making any crop improvement program successful. DNA fingerprinting technology to assess the genetic relationship among the selected genotypes for identification and cataloging of different species and cultivars of roses is a promising tool for Rosa genomes. The inter-simple sequence repeats markers (ISSRs) were used to investigate the genetic diversity among twenty-one diverse Rosa genotypes belonging to two different species, Rosa hybrida and R. damascena, and three distinct groups of rose varieties, namely Hybrid Tea, Floribunda, and Damask roses. Twenty-four ISSR primers yielded a total of 280 scorable amplified fragments from 250-1800 bp in length, from which 244 were polymorphic, resulting in an average of 86.4% polymorphism. UPGMA cluster analysis based on Jaccard’s pairwise similarity coefficient values ranged from 0.264 to 0.818, clearly distinguished different species and genotypes, grouping them into three distinct clusters. The results confirmed a high degree of variation in the rose germplasm studied highlighting the potential of improvement in roses for the ornamental and perfume industry.
Keywords
ISSR; Rosa spp.; DNA fingerprinting; genotypes; genetic diversity
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
