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Preprint ARTICLE | doi:10.20944/preprints202309.0323.v1

utPCR: A Strategy for Highly Specific and Absolutely Quantitative Detection of Single Molecules within Only Minutes

Rui Wang, Ying Liu, Shuaiwei Chen, Linlin Bai, Kaiming Guo, Yanan Pang, Feng Qian, Yongfang Li, Li Ding, Yongming Wang

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Ultrafast PCR; droplet digital PCR (ddPCR); single-molecule detection; bloodstream infection; absolute quantification; molecular diagnosis

Online: 6 September 2023 (04:20:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0361.v1

Novel Microfluidic Septum to Optimize Energy Recovery in Single Chamber Microbial Fuel Cells

Giacomo Spisni, Giulia Massaglia, Valentina Bertana, Nicolò Vasile, Candido Fabrizio Pirri, Stefano Bianco, Marzia Quaglio

Subject: Physical Sciences, Applied Physics Keywords: Microbial fuel cell; Microfluidics; Fluidic separator; Single Chamber Microbial Fuel Cell; Energy Recovery optimization

Online: 6 September 2023 (03:45:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0290.v1

Intelligent Image Recognition System for Preventing Wrong Side Upper Limb Surgery

Yi-Chao Wu, Chao-Yun Chang, Yu-Tse Huang, Sung-Yuan Chen, Cheng-Hsuan Chen, Hsuan-Kai Kao

Subject: Engineering, Bioengineering Keywords: Intelligent Image Recognition; Left and Right Upper Limb Dislocation Surgery; Accuracy Rate; Recall Rate; IRB

Online: 5 September 2023 (09:20:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202309.0015.v1

A Comprehensive Genetic Study of Microtubule-Associated Genes Clusters for Male Infertility in a Taiwanese Cohort

Chying-Chyuan Chan, Te-Hsin Yen, Hao-Chen Tseng, Brang Mai, Pin-Kuan Ho, Jian-Liang Chou, Gwo-Jang Wu, Yu-Chuan Huang

Subject: Medicine And Pharmacology, Reproductive Medicine Keywords: male infertility; azoospermia; oligozoospermia; next-generation sequencing; single nucleotide polymorphism; microtubule-associated genes

Online: 1 September 2023 (13:38:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1905.v1

A Simplified Design Method for Quasi Resonant Inverter Used in Induction Hob

Metin Ozturk

Subject: Engineering, Electrical And Electronic Engineering Keywords: Home appliances; induction cooking systems; induction cooker design; resonant converter circuit parameters; single switch quasi resonant inverter

Online: 29 August 2023 (08:47:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.1932.v1

Recent Advances of Single-atom Metal Supported at Two-dimensional MoS2 for Electrochemical CO2 Reduction and Water Splitting

Jiahao Wang, Xiaorong Gan, Tianhao Zhu, Dan Zhao, Yanhui Ao, Peifang Wang

Subject: Environmental And Earth Sciences, Environmental Science Keywords: single-atom metal; two-dimensional nanomaterials; water splitting; CO2 reduction; catalysis; DFT

Online: 29 August 2023 (08:42:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1843.v1

Passivation of Defective States in Single Crystal MAPbBr3 and Their Optoelectronic Properties Study

Hui Li, Jin Wang, Haitao Li, Zhi Guo, Zhenhua Chen

Subject: Physical Sciences, Applied Physics Keywords: single crystal; perovskite; synchrotron; MAPbBr3; optical property

Online: 28 August 2023 (09:52:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.1809.v1

Advanced Omics Techniques for Understanding Cochlear Genome, Epigenome and Transcriptome in Health and Disease

Annamaria Tisi, Sakthimala Palaniappan, Mauro Maccarrone

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Omics, Cochlea, Single cell omics, Spatial omics, Epigenomics, Transcriptomics, Genomics, Organ of Corti.

Online: 25 August 2023 (13:29:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.1664.v1

Limb Salvage and Survival in Chronic Limb-Threatening Ischemia: The Need for Fast-Track Team-Based Approach

Giorgio Ventoruzzo, Giulia Mazzitelli, Umberto Ruzzi, Francesco Liistro, Alessia Scatena, Eugenio Martelli

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: peripheral arterial disease; critical limb ischemia; chronic limb-threatening ischemia; survival; mortality; limb salvage

Online: 25 August 2023 (02:41:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1500.v1

Rapid Evolutionary Adaptation to Diet Composition in Black Soldier Fly (Hermetia illucens)

Anton Gligorescu, Long Chen, Kim Jensen, Neda Nasiri Moghadam, Torsten N Kristensen, Jesper Givskov Sørensen

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: evolutionary adaptation; insect production, low-quality diets; single-sourced waste streams; tradeoffs

Online: 22 August 2023 (07:34:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1259.v1

Growing in saltwater: biotechnological potential of novel Methylotuvimicrobium- and Methylomarinum-like methanotrophic bacteria

Ekaterina N. Tikhonova, Ruslan Z. Suleimanov, Igor Y. Oshkin, Aleksey A. Konopkin, Diana V. Fedoruk, Nikolai V. Pimenov, Svetlana N. Dedysh

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: methanotrophic bacteria; Methylomonarinum; Methylotuvimicrobium; single-cell protein; growth in seawater; genome analysis.

Online: 17 August 2023 (08:01:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1030.v1

A New CNN-based Single-Ingredient Classification Model and Its Application in Food Image Segmentation

Ziyi Zhu, Ying Dai

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: CNN architecture; Single Ingredient Classification model; Food Ingredients segmentation; evaluation metrics; Hierarchical Multi-level learning

Online: 14 August 2023 (11:20:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0861.v1

Energy Gap-Refractive Index Relations in Semiconductors − Using Wemple-DiDomenico model to unify Moss, Ravindra, and Herve-Vandamme Relationships

Aneer Lamichhane

Subject: Physical Sciences, Optics And Photonics Keywords: energy gap; refractive index; Moss, Ravindra, Herve-Vandamme relationships; Wemple and DiDomenico single oscillator model

Online: 11 August 2023 (02:56:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0832.v1

Puppis: Hardware Accelerator of Single-Shot Multibox Detectors for Edge-Based Applications

Vladimir Vrbaski, Slobodan Josic, Vuk Vranjkovic, Predrag Teodorovic, Rastislav Struharik

Subject: Engineering, Electrical And Electronic Engineering Keywords: Hardware acceleration; Convolutional Neural Networks; Single-Shot Multibox Detector

Online: 10 August 2023 (10:25:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0266.v1

Effect of Fiber Sizing Levels on the Mechanical Properties of Carbon Fiber Reinforced Thermoset Composites

Albraa A. Jaber, Sara Abbas, Abdiaziz A. Farah, Karina Kopec, Yahya Al-Salik, Mohammed Tayeb, Nikhil Verghese

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: Carbon fiber; thermoset; sizing; single fiber tensile testing; single fiber pullout testing; sizing levels; X-ray photoelectron spectroscopy (XPS)

Online: 3 August 2023 (10:52:01 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.0175.v1

Review and Analysis of Platform-Related Performance of Rehabilitation Lower Limb Exoskeletons

Hossein Karami, Stefano Maludrottu, Christian Vassallo, Matteo Laffranchi, Lorenzo De Michieli

Subject: Engineering, Bioengineering Keywords: Powered lower limb exoskeletons; rehabilitation and assistance; performance criteria; neurological disorders; auxiliary devices; control strategies; effectiveness of PLLE

Online: 2 August 2023 (10:04:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0182.v1

Multipronged Approach to Profiling Metabolites in Beta Vulgaris L Dried Pulp Extracts Using Chromatography, NMR, and Other Spectroscopy Methods

Joshua Fiadorwu, Kiran Subedi, Daniel A Todd, Mufeed M Basti

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Beetroot (Beta vulgaris); Total Correlation Spectroscopy (TOCSY); Gas Chromatog-raphy-Mass Spectrometry (GC-MS); selective TOCSY; Homonuclear single quantum correlation (HSQC); fatty acid methyl esters (FAME)

Online: 2 August 2023 (10:01:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.0083.v1

Electrical Manipulation, Characterization and Application of Single Cell

Weitao Zhu, Jiaao Wang, Hongzhi Luo, Sutong Liu, Binwen Luo, Xue Li, Shan Liu, Chenzhong Li

Subject: Engineering, Bioengineering Keywords: single-cell analysis; electrical manipulation; single-cell application

Online: 1 August 2023 (10:50:09 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202308.0079.v1

Influence of Different Evolutive Forces on GDF5 Gene Variability

Laura Flore, Paolo Francalacci, Myosotis Massidda, Renato Robledo, Carla Maria Calò

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Single nucleotide polymorphism, Population Branch Statistic, selective pressure.

Online: 1 August 2023 (10:37:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1799.v2

QTL Epistasis Plays a Role of Homeostasis on Heading Date in Rice

Lilong Huang, Jichun Tang, Bihuang Zhu, Guodong Chen, Leyi Chen, Suhong Bu, Haitao Zhu, Zupei Liu, Zhan Li, Lijun Meng, Guifu Liu, Shaokui Wang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Epistasis; Homeostasis; Single segment substitution line; Heading date; Rice

Online: 31 July 2023 (09:42:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1587.v1

Timing and Performance Metrics for TWR-K70F120M device

George K. Adam

Subject: Computer Science And Mathematics, Computer Science Keywords: single board computers; embedded systems; real-time; multithreading; performance metrics; time measurements; benchmarking; μClinux; TWR-K70F120M

Online: 24 July 2023 (09:53:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1417.v1

Phenotypic Differences among Recently Diagnosed Drug-Naïve Patients with Parkinson’s Disease with or without SNCA Polymorphisms

Santiago Pérez-Lloret, Alejandro Enet, Guenson Chevalier, Sofia Bordet, Hanny Barbar, Francisco Capani, Lucas Udovin, Matilde Otero-Losada, Francisco Barrantes

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: single nucleotide polymorphism; Parkinson’s disease; alpha-synuclein; phenotype, pathophysiology; PD-related variants

Online: 20 July 2023 (12:46:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1116.v1

Impact of Environmental Sub-Inhibitory Concentrations of Antibiotics, Heavy Metals, and Biocides on the Emergence of Tolerance and Effects on the Mutant Selection Window in E. coli

Kelechi B. Chukwu, Ovokeroye A. Abafe, Daniel G. Amoako, Arshad Ismail, Sabiha Y. Essack, And Akebe L. K. Abia

Subject: Public Health And Healthcare, Public, Environmental And Occupational Health Keywords: environmental stressors; pharmaceuticals; antibiotic resistance; selection pressure; public health; tolerant bacteria; environmental pollution; mutation; whole genome sequencing; single nucleotide polymorphisms

Online: 18 July 2023 (05:36:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1022.v1

Analyses of Subsample Averaging of Single Trials for Event-Related Potential Classification

Xiaoqian Chen, Lalit Gupta

Subject: Computer Science And Mathematics, Signal Processing Keywords: ERP classification; single trial averaging; interclass separation; convolution neural networks; support vector machines.

Online: 17 July 2023 (02:32:36 CEST)

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Event-related potentials (ERPs) are estimated by averaging time-locked single trial electroencephalography (EEG) signals in response to specific events or stimuli. Classifying ERPs accurately is a challenge because (a) single trials have poor signal-to-noise-ratios (SNRs) and (b) it is difficult to collect large single trial ensembles to generate high SNR ERPs for classifier training and testing. The m-subsample averaging (m-SA) strategy which generates small-sample ERPs by repeated averaging of a small number of single trials drawn without replacement, has been proposed as a solution to the two problems. An ERP formed by averaging m single trials is referred to as an m-ERP where m is referred to as the averaging parameter. In this study, we conduct thorough analyses of m-SA and focus on issues not addressed in previous studies to better understand the beneficial properties of m-SA and to further support its application for ERP classification. Specifically, we (a) analyze the improvement in SNR as a function of m using the mean-root-mean-square SNR and visual analyses of m-ERP plots with confidence intervals, (b) analyze the improvement in interclass separation as a function of m, (c) determine how the SNR and interclass separation analyses can help to select the averaging parameter m, (d) determine the number of distinct m-ERPs that can be drawn from a single-trial ensemble, and (e) determine several probabilities related to the generation of distinct m-ERPs. Furthermore, an extensive set of experiments are designed to analyze the performance of support vector machine and convolution neural network classifiers employing m-SA with various combinations of the averaging parameters used for generating the training and test sets. The results confirm that ERPs can be classified accurately using small subsample averaging. Most importantly, it is concluded that m-SA can be deployed in practice to accurately classify ERPs in brain activity research and in clinical applications without having to collect a prohibitively large number of single trials.

Preprint ARTICLE | doi:10.20944/preprints202307.1008.v1

Patient Reported Outcome Measures (PROMs) Amongst Lower Extremity Agonist-Antagonist Myoneural Interface (AMI) Amputees

Rachael B Chiao, Corey L Sullivan, Lori Berger, Tawnee L Sparling, Tracy L Landry, Kendall Clites, Matthew J Carty

Subject: Medicine And Pharmacology, Surgery Keywords: Transtibial amputation; transfemoral amputation; agonist-antagonist myoneural interface; AMI; proprioception; neural interface; functional limb restoration; patient reported outcomes

Online: 14 July 2023 (13:29:21 CEST)

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Background: The standard surgical approach to amputation has failed to evolve significantly over the past century. Consequently, standard amputations often fall short with regards to improving the quality-of-life (QoL) for patients. A modified amputation has been designed at both the transtibial (TTA) and transfemoral (TFA) level that incorporates agonist-antagonist myoneural interface (AMI) constructs to provide patients with a novel alternative to standard amputation. Here, we present the patient reported outcome measures (PROMs) amongst our lower extremity AMI amputees and demonstrate an overall significant improvement in their physical and mental wellbeing. Methods: Four PROMs surveys, 1) EQ-5D-3L, 2) Lower Extremity Functional Scale (LEFS), 3) PROMIS-57, 4) Short Form-36 (SF-36), and 5) Sickness Impact Profile (SIP), were administered to lower extremity AMI amputees pre-operatively (baseline) and at 6 weeks, 3 months, 6 months, 9 months, 12 months, 24 months, 36 months, and 48 months post-operatively between June 24th, 2018, and December 15th, 2022. The surveys were either administered during a clinic visit or emailed at the encounter timepoint. Study data was collected and managed using REDCap (Research Electronic Data Capture) electronic data capture tools hosted at Brigham and Women’s Hospital. Analyses of collated data were performed by fitting a linear mixed model using SAS version 9.4 (SAS Institute, Cary, NC). Results: The cohort’s baseline and 12-month post-operative responses were compared to determine score improvement. The cohort demonstrated a significantly improved EQ-5D-3L index value (p<0.0001) and EQ VAS (p<0.0001) at 12-months post-operatively (n=22) when compared to baseline (n=28). The LEFS score significantly improved at 12-months post-operatively compared to baseline (p<0.0001) with a mean of 59.04 ± 3.14 (SE) at 12-months and a mean of 31.96 ± 2.84 (SE) at baseline. AMI patients demonstrated significantly improved scores across PROMIS-57 domains at 12-months compared to baseline scores; the p-value was <0.0001 for all domains except for the Depression domain where p=0.0181. The health domain scores significantly improved from baseline to 12-months post-operation for all SF-36 domains. Lastly, the SIP indicated a significant decrease in overall dysfunction from baseline to 12-months post-operation (p<0.0001). Conclusions: Modified lower extremity amputation with AMI construction has the potential to provide amputees with increased quality-of-life when compared to the pre-operative state. However, further investigation is necessary to determine whether the patient reported outcome measures of the AMI amputee cohort are superior to those who receive a standard amputation.

Preprint ARTICLE | doi:10.20944/preprints202307.0862.v1

Skeletal Space Structure Systems: Select Areas of Opportunity to Achieve Sustainability in Construction

Shahrokh Maalek, Reza Maalek, Bahareh Maalek

Subject: Engineering, Civil Engineering Keywords: space structure systems; composite structures; double- and single-layer grid structures; sustainability in design; mass customization; kit-of-parts; digital engineering and construction

Online: 13 July 2023 (03:12:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0850.v1

Disentangling the Economic and Educational Impacts of Single Parenting on Adolescent Girl Students: In the Case of Gambella Region, Ethiopia

Fentahun Gebrie, Surafel Melsew

Subject: Social Sciences, Gender And Sexuality Studies Keywords: Single Parenting; Adolescent Girls; Economic Impact; Academic Performance; Gambella; Ethiopia

Online: 12 July 2023 (12:12:11 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0807.v3

Countering and Tackling Advanced First-Line IMMUNE Evasion: The Most Feasible and Precise Approach to Control and Eradicate Rabies?

Theodor-Nicolae Carp

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: rabies; RABV; PRV; single-stranded RNA; RNA-dependent RNA Polymerase; viral self-camouflaging; glycoprotein; innate immunity; interferon system; natural lymphocytes; adaptive immunity; adaptive lymphocytes; dendritic cells; IgA; IgM; IgG; primary dendritic cells; macrophages

Online: 10 July 2023 (10:09:23 CEST)

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Despite being a rare disease worldwide, rabies has the highest morbidity and mortality rates, with nearly all symptomatic cases leading to coma and death. Rabies represents an infectious disease caused by the Rabies virus (RABV), which is part of the Lyssavirus group and the Rhabdoviridae family, and it mainly spreads through the bite and scratch of an infected mammal, but particularly of wild animals, such as bats, foxes, wolves and racoons, and of domestic animals, such as dogs and cats, in rabies-prone areas of the world. Airborne transmission has been deemed as extremely rare, and no clinical case as such has been recorded worldwide yet, except in the enclosed environment, such as research laboratories and caves where infected bats are present. Domestic mammals, such as dogs and ferrets, represent other important reservoirs of disease transmission, and the human cases of Asia and Africa amount approximately 95% of all human cases worldwide. Infected animals most commonly start transmitting the virus once the first symptoms have occurred, and if they experience disease aggravation and death within 10 days, a case of rabies is registered, more easily if the incidence occurred in the urban area and then, any person or animal that had been potentially exposed are strongly recommended to receive the inoculation. It is rare for asymptomatic mammals to transmit the illness. Most First-World and several Second-World countries have recently been declared dog rabies-free by the World Health Organization. The disease can only be treated prophylactically, with three doses of a vaccine containing an inactivated form of RABV, or with five doses of the vaccine and two doses of anti-RABV immunoglobulins within 28 days if the patient is believed to have been exposed to the virus beforehand. It has been projected that, once the viral load reaches elements of the central nervous system, prophylactic approaches are no longer effective, even if symptoms have not begun yet, and this highlights the urgent trait of the medical condition, strongly recommending exposed people to receive the prophylactic doses immediately after the potential exposure to the virus. The pathogen first infects the bodily fluids, before reaching the peripheral nervous system, from where it will gradually move toward the spinal cord or the encephalon, at a speed of movement ranging from 1 to 40 cm per day. It was also found, in extremely rare circumstances, to infect the nasopharyngeal cavity and the lungs. The primary cause of a successful, gradual advance of the viral load toward the point of clinical no-return for the patient - the CNS - is a complex mechanism of induced innate immune evasion, with the interferon system being heavily targeted and silenced by RABV proteins. The ‘Milwaukee’ protocol is locally believed to decrease the mortality rate of the clinical illness to approximately 80%, although significantly more research is required in this sense. First-line immune evasion represents the central mechanism developed by viruses during their evolutionary process to gain control over human immunity, so it could be the development and adjustment of a counter-offensive to this evolutionary operating system that could address the core elements of the problem. Human recombinant Type I and Type III Interferons were found to be significant vaccine adjuvants and to considerably delay the clinical onset of the disease. Despite their central role in natural immunity-based prophylaxis, vaccine support and, in often cases, vaccination per se, a local administration of IFNs as such may not be enough to tackle the core problem of the endemic disease, and a specific and systemic treatment of potential host cells with IFN I and III, as well as IFN-stimulating proteins, may constitute a major research requirement in the coming years of disease investigation, as the inoculation efforts with the inactivated virus and immunoglobulin administration continue. The administration of a relatively low dosage of somatic Natural Killer cells, gamma-interferon and perhaps, of somatic helper CD4+ and somatic cytotoxic CD8+ T-lymphocytes treated with alpha-, beta- and lambda-interferon could be merged with the administration of a similar dosage of alpha-, beta- and lambda-interferon during the efforts to develop an effective and less costly prophylactic vaccine against rabies. A combination of a nasal substance containing a low dosage of IFN I and III with a reduced concentration of neutralized RABV copies, and/or with a low dose of anti-RABV IgA antibodies, could also be tested for humans for the purposes of pre- and post-exposure prophylaxis.

Preprint ARTICLE | doi:10.20944/preprints202307.0410.v1

Carbonized Melamine Cyanurate as a Palladium Catalyst Support for the Dehydrogenation of N-heterocyclic Compounds in LOHC Technology

Anton P. Koskin, Yurii V. Larichev, Sergey A. Stepanenko, Yury V. Dubinin, Artem B. Ayupov, Evgeny A. Suprun, Petr M. Yeletsky

Subject: Chemistry And Materials Science, Chemical Engineering Keywords: g-C3N4; melamine cyanurate; Pd catalysts; single atom catalysts; hydrogen storage; LOHC; dehydrogenation

Online: 6 July 2023 (10:22:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0139.v1

Spatial Transcriptomic Analysis of Focal and Normal Areas of Myocyte Disarray in Human Hypertrophic Cardiomyopathy

Jason Laird, Gayani Perera, Rebecca Batorsky, Hongjie Wang, Knarik Arkun, Michael T. Chin

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Hypertrophic Cardiomyopathy; spatial transcriptomics; single nucleus RNA-sequencing; gene expression; bioinformatics; cardiovascular disorder; genetic disorder

Online: 4 July 2023 (05:18:51 CEST)

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Hypertrophic Cardiomyopathy (HCM) is a common inherited disorder that can lead to heart failure and sudden cardiac death, characterized at the histological level by focal areas of myocyte disarray, hypertrophy and fibrosis, and few disease-targeted therapies exist. To identify, focal, spatially restricted alterations in transcriptional pathways and reveal novel therapeutic targets, we performed a spatial transcriptomic analysis of areas of focal myocyte disarray compared to areas of normal tissue, using a commercially available platform (GeoMx, nanoString). We analyzed surgical myectomy tissue from four patients with HCM and control interventricular septum tissue from two unused organ donor hearts that were free of cardiovascular disease. Histological sections were reviewed by an expert pathologist and 72 focal areas with varying degrees of myocyte disarray (normal, mild, moderate, severe) were chosen for analysis. Areas of interest were interrogated with the Human Cancer Transcriptome Atlas designed to profile 1800 transcripts. Differential expression analysis revealed significant changes in gene expression between HCM and Control tissue, and functional enrichment analysis indicated these genes were primarily involved in interferon production and mitochondrial energetics. Within HCM tissue, differentially expressed genes between areas of mild and moderate disarray were enriched for genes related to mitochondrial energetics (moderate disarray) and response to oxygen/cytokine levels (mild disarray). The comparison between areas of moderate and severe disarray were enriched for genes related to the c-Jun N-terminal kinase (JNK) cascade in severe disarray. Analysis of ligand-receptor pair gene expression revealed that HCM tissue exhibited downregulation of platelet-derived growth factor (PDGF), NOTCH, junctional adhesion molecule, and CD46 signaling, while showing upregulation of fibronectin, CD99, cadherin, and amyloid precursor protein signaling. A deconvolution analysis utilizing the matched single nuclei RNA-sequencing (snRNA-seq) data to determine cell type composition in areas of interest revealed significant differences in fibroblast and vascular cell composition in areas of severe disarray when compared to normal areas in HCM samples. Cell composition in normal areas from control tissue was also divergent from normal areas in HCM samples, which was consistent with the differential expression results. Overall, our data identify novel and potential disease-modifying targets for therapy in HCM.

Preprint ARTICLE | doi:10.20944/preprints202306.2112.v1

Risk Factors and Outcomes of Children with Congenital Heart Disease on Extracorporeal Membrane Oxygenation, a 10-Year Single-Center Report

Antonio Amodeo, Milena Stojanovic, Tugba Erdil, Hitendu Dave, Robert A. Cesnjevar, Sebastian Paal, Oliver Kretschmar, Martin Schweiger

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: ongenital heart disease; extracorporeal life support; ECMO; single ventricle; complications; risk factors; mortality

Online: 30 June 2023 (03:41:18 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202306.2148.v1

The Homoleptic Curcumin-Copper Single Crystal (ML2): A Long Awaited Cherry on the Cake in the Field of Curcumin Metal Complexes

Antonino Arenaza Corona, Marco A. Obregón Mendoza, William Meza Morales, María Teresa Ramírez Apan, Antonio Nieto Camacho, Rubén A. Toscano, Leidys L. Pérez González, Rubén Sánchez Obregón, Raúl G. Enríquez

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: Curcumin-Copper (II); single crystal; cytotoxicity; antioxidant

Online: 29 June 2023 (13:26:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2066.v1

Age-Friendly Approach Is a Necessary Condition to Prevent Depopulation: The Age-Friendliness Evaluation of Japanese Municipalities by Resident Architectural Designers and Constructors

Kazumasa Yamada, Kenta Murotani, Makiko Mano, Youngmi Lim, Jun Yoshimatsu

Subject: Social Sciences, Demography Keywords: age-friendliness; aging society; rural aging; urban aging; demographic aging; depopulation; land price; single person household; outdoor spaces; Japan

Online: 29 June 2023 (05:20:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2047.v1

The Genetic Basis of Probable REM Sleep Behavior Disorder in Parkinson’s Disease

Santiago Pérez-Lloret, Guenson Chevalier, Sofia Bordet, Hanny Barbar, Francisco Capani, Lucas Udovin, Matilde Otero-Losada

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: single nucleotide polymorphism; Parkinson’s disease; REM-sleep Behaviour Disorders; pathophysiology; PD-related variants

Online: 28 June 2023 (14:07:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1881.v1

Hospital Readmission of Patients with Diabetic Foot Ulcers: Prevalence, Causes and Outcomes

Marco Meloni, Aikaterini Andreadi, Valeria Ruotolo, Maria Romano, Ermanno Bellizzi, Laura Giurato, Alfonso Bellia, Luigi Uccioli, Davide Lauro

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: diabetes; diabetic foot; hospital admission; limb salvage

Online: 27 June 2023 (10:18:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1603.v1

Single Cell Transcriptomic Analysis in a Mouse Model of Barth Syndrome Reveals Cell-Specific Alterations in Gene Expression and Intercellular Communication

Gayani Perera, Liam Power, Amy Larson, Christina J. Codden, Junya Awata, Rebecca Batorsky, Douglas Strathdee, Michael T. Chin

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Tafazzin; Barth Syndrome; Single-Nucleus RNA-sequencing; Cardiomyopathy; Mitochondria; Gene Expression; Metabolism

Online: 22 June 2023 (11:31:02 CEST)

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Barth Syndrome, a rare X-linked disorder affecting 1:300,000 live births, results from defects in Tafazzin, an acyltransferase that remodels cardiolipin and is essential for mitochondrial respiration. Barth Syndrome patients develop cardiomyopathy, muscular hypotonia and cyclic neutropenia during childhood, rarely surviving to middle age. At present, no effective therapy exists and downstream transcriptional effects of Tafazzin dysfunction are incompletely understood. To identify novel, cell-specific, pathological pathways that mediate heart dysfunction, we performed single-nucleus RNA-sequencing (snRNA-seq) on wild-type (WT) and Tafazzin-knockout (Taz-KO) mouse hearts. We determined differentially expressed genes (DEGs) and inferred predicted cell-cell communication networks from these data. Surprisingly, DEGs were distributed heterogeneously across the cell types, with fibroblasts, cardiomyocytes, endothelial cells, macrophages, adipocytes and pericytes exhibiting the greatest number of DEGs between genotypes. One differentially expressed gene was detected for the lymphatic endothelial and mesothelial cell types, while no significant DEGs were found in the lymphocytes. A Gene Ontology (GO) analysis of these DEGs showed cell-specific effects on biological processes such as fatty acid metabolism in adipocytes and cardiomyocytes, increased translation in cardiomyocytes, endothelial cells and fibroblasts, in addition to other cell-specific processes. Analysis of ligand-receptor pair expression, to infer intercellular communication patterns, revealed the strongest dysregulated communication involved adipocytes and cardiomyocytes. For the knockout hearts, there was a strong loss of ligand-receptor pair expression involving adipocytes, and cardiomyocyte expression of ligand-receptor pairs underwent reorganization. These findings suggest that adipocyte and cardiomyocyte mitochondria may be most sensitive to mitochondrial Tafazzin deficiency and that rescuing adipocyte mitochondrial dysfunction, in addition to cardiomyocyte mitochondrial dysfunction, may provide therapeutic benefit in Barth Syndrome patients.

Preprint ARTICLE | doi:10.20944/preprints202306.1383.v1

Test-Retest Repeatability of Human Gestures in Manipulation Tasks

Elisa Digo, Elena Caselli, Michele Polito, Mattia Antonelli, Laura Gastaldi, Stefano Pastorelli

Subject: Engineering, Mechanical Engineering Keywords: Industry 5.0; MIMUs; collaborative robotics; upper limb; human-machine interaction

Online: 20 June 2023 (03:41:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1343.v1

Dancers’ Physical Fitness across their Four-Year Collegiate Programs: A Prospective Analysis

Jatin Prafull Ambegaonkar, Jena Hansen-Honeycutt, Kelley Rock Wiese, Catherine M Cavanagh, Shane V Caswell, Shruti J Ambegaonkar, Joel Martin

Subject: Public Health And Healthcare, Physical Therapy, Sports Therapy And Rehabilitation Keywords: Performing Arts; Core; Star Excursion Balance Test; Balance; Push-ups; Planks; Single Leg Hops; Dancers; Training; Performance; Health; Physical Fitness; collegiate

Online: 19 June 2023 (10:13:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1276.v1

A Concise Review of Single-Molecule Magnets, Carbon Nanotubes and Hybrids Between Them

Mudasir A Yatoo, Faiza Habib, Ahmad Husain, Sharique Ahmad, Zubair Ahmad

Subject: Chemistry And Materials Science, Inorganic And Nuclear Chemistry Keywords: Molecular magnetism; single-molecule magnets; carbon nanotubes; single-molecule magnets-carbon nanotubes hybrids.

Online: 19 June 2023 (03:38:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1079.v1

Virtual Screening of Different Subclasses of Lignans with Anticancer Potential and Based on Genetic Profile

Mayara dos Santos Maia, Francisco Jaime Bezerra Mendonça-Junior, Gabriela Cristina Soares Rodrigues, Adriano Soares da Silva, Niara Isis Pereira de Oliveira, Pablo Rayff da Silva, Cícero Francisco Bezerra Felipe, Ana Pavla Almeida Diniz Gurgel, Anuraj Nayarisseri, Marcus Tullius Scotti, Luciana Scotti

Subject: Medicine And Pharmacology, Pharmacy Keywords: lignans; cancer; single nucleotide polymorphisms; virtual screening

Online: 16 June 2023 (05:10:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1035.v1

Integrating Single-Cell RNA-Seq and Bulk RNA-Seq to Explore the Key Role of Fatty Acid Metabolism in Breast Cancer

Yongxing Chen, Chenxin Jin, Jiaxue Cui, Yizhuo Diao, Ruiqi Wang, Rongxuan Xu, Zhihan Yao, Wei Wu, Xiaofeng Li

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Fatty Acid Metabolism; Immunotherapy; Breast cancer; Tumor microenvironment; Single-cell sequencing

Online: 14 June 2023 (09:40:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0983.v1

Lanthanide Complexes with Tripodal Ligands

Kira E. Vostrikova

Subject: Chemistry And Materials Science, Inorganic And Nuclear Chemistry Keywords: tripodal ligands; lanthanides; single-molecule magnets; single-ion magnets; axiality

Online: 14 June 2023 (04:47:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0958.v1

Investigation of Single Event Upset in Graphene Nano-Ribbon FET SRAM Cell

Naheem Olakunle Adesina

Subject: Engineering, Electrical And Electronic Engineering Keywords: graphene nano-ribbon FET; single event upset; SRAM; stability; FinFET

Online: 13 June 2023 (16:22:35 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0916.v1

Recent Advances on Electrospun Fibers for Biological Applications

Bénédicte Fromager, Emilie Marhuenda, Benjamin Louis, Norbert Bakalara, Julien Cambedouzou, David Cornu

Subject: Chemistry And Materials Science, Biomaterials Keywords: electrospinning, single needle, multi-needle, polyacrylonitrile, nanofibers, parameters, scaffold, biological application, tissues engineering, cell culture.

Online: 13 June 2023 (09:57:19 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0684.v1

The Isokinetic Method in Female Uninjured Female Soccer Players: A Survey of Applications and Common Mismatches

Antonio Cicchella, Cheng Zhang

Subject: Medicine And Pharmacology, Anatomy And Physiology Keywords: Female soccer; isokinetics; hamstring/quadriceps ratio; dominant non dominant limb ratio; errors in isokinetic dynamometry

Online: 9 June 2023 (08:17:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1684.v3

Reference Values for the Grocery Shelving Test among United Arab Emirates Population: A Cross Sectional Study

Gopala Krishna Alaparthi, Aishwarya Prabhakar Gatty, Huda Isa, Latifa Hassan, Noura Khalid, Abdullah Osman, Sara Mahmoud, May Tamim, Kalyana Chakravarthy Bairapareddy, Arthur De Sa Ferreira

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: Arm elevation; Grocery Shelving Test; Functional assessment; Reference values; Upper limb activities

Online: 8 June 2023 (08:52:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0605.v1

Synthesis and Evaluation of 99mTc-labeled PSMA-targeted Tracers Based on the Lys-urea-Aad Pharmacophore for Detecting Prostate Cancer with Single Photon Emission Computed Tomography

Kelly Lu, Chengcheng Zhang, Zhengxing Zhang, Hsiou-Ting Kuo, Nadine Colpo, François Bénard, Kuo-Shyan Lin

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: prostate-specific membrane antigen (PSMA); technetium-99m; single photon emission computed tomography (SPECT); molecular imaging; HYNIC

Online: 8 June 2023 (08:11:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0531.v1

Construction of A Bis(benzene sulfonyl)imide Based Single-ion Polymer Artificial Layer for Steady Lithium Metal Anode

Yujie Wang, Mengmeng Zhao, Yazhou Chen, Haifeng Bao, Chen Li

Subject: Chemistry And Materials Science, Surfaces, Coatings And Films Keywords: Lithium metal battery; lithium metal anode; polymeric artificial layer; single ion conductor; lithium dendrite.

Online: 7 June 2023 (09:43:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0486.v1

A Flexible and Transparent PtNP/Swcnt/Pet Electrochemical Sensor for Nonenzymatic Detection of Hydrogen Peroxide Released From Living Cells With Real-time Monitoring Capability

Da Eun Oh, Chang-Seuk Lee, Tae Wan Kim, Seob Jeon, Tae Hyun Kim

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: Electrochemical sensing; flexible sensor; hydrogen peroxide (H2O2); platinum nanoparticles; single-walled carbon nanotube (SWCNT) network

Online: 7 June 2023 (04:26:10 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0392.v1

Review of Single Crystal Synthesis of 11 Iron-Based Superconductors

Qiang Hou, Longfei Sun, Yue Sun, Zhixiang Shi

Subject: Physical Sciences, Condensed Matter Physics Keywords: single crystal; hydrothermal; CVT; self-flux; annealing; 11 iron-based superconductors; superconductivity

Online: 6 June 2023 (07:11:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0317.v1

Parental Burnout in the Context of Special Needs, Adoption, and Single Parenthood

Isabelle Roskam, Moïra Mikolajczak

Subject: Social Sciences, Psychology Keywords: exhaustion; disability; illness; disorder; adopted child; adoptive parent; single mom; single dad

Online: 5 June 2023 (12:07:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0275.v1

Understanding the Impact of COVID-19 on Angioplasty Service and Outcome of Patients Treated for Critical Limb Ischaemia: A Single-Centre Retrospective Cohort Study

Alexander D. Rodway, Jenny Harris, Lydia Hanna, Charlotte Allan, Felipe Pazos-Casal, Ciara Giltinan, Ali Dehghan-Nayeri, Andre Santos, Martin B. Whyte, Nikolaos Ntagiantas, Ivan Walton, Richard Brown, Simon S. Skene, Ajay Pankhania, Benjamin C.T. Field, Gary D. Maytham, Christian Heiss

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: day-case angioplasty; critical limb ischaemia; peripheral artery disease

Online: 5 June 2023 (08:37:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0199.v1

Predicting the Effect of Mutations on Protein Stability and Binding: Assessment of Leading Algorithms Performance and Databases Content with Respect to Types of Mutations

Preeti Pandey, Shailesh Pandey, Prawin Rimal, Nicolas Ancona, Emil Alexov

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: mutations; folding free energy change; binding free energy change; single nucleotide variant

Online: 2 June 2023 (11:58:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0138.v1

Study of 6√3 Reconstruction Conversion on the of 4H-SiC(0001) Surface to Quasi-free Epitaxial Graphene with Electron Diffraction

Irina Sergeevna Kotousova, Sergey Pavlovich Lebedev, Vladimir Viktorovich Antipov, Alexander Alexandrovich Lebedev

Subject: Physical Sciences, Condensed Matter Physics Keywords: silicon carbide; RHEED; reconstruction; hydrogen intercalation; quasi-free single-layer graphene

Online: 2 June 2023 (10:03:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2217.v1

Injury Prevalence among Young Elite Baseball Players

Daeho Ha, Satoshi Nagai, Byungjoo Noh, Naoki Mukai, Shumpei Miyakawa, Masahiro Takemura

Subject: Medicine And Pharmacology, Orthopedics And Sports Medicine Keywords: epidemiology; baseball injury; young baseball players; injury prevalence; early single sport specialization

Online: 31 May 2023 (10:06:25 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.2170.v1

UV-DDB as a General Sensor of DNA Damage in Chromatin: A Direct Role in Base Excision Repair

Sripriya J Raja, Bennett Van Houten

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: DNA damage; base excision repair; nucleotide excision repair; UV-DDB; chromatin; nucleosome; DNA glycosylases; single molecule; cell biology; biochemistry

Online: 31 May 2023 (05:44:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1780.v1

Molecular Mapping To Identify Stable Salinity-Resilient QTLs From the Novel Landrace Akundi in Two Bi-Parental Populations Using SNP-Based 1k-RiCA Genotyping in Rice

Sheikh Maniruzzaman, M. Akhlasur Rahman, Mehfuz Hasan, Golam Golam Rasul, Abul Hossain Molla, Hasina Khatun, KM Md Iftekharuddaula, Salma Akter

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: genetic loci; salt tolerant indica germplasm (Akundi); single nucleotide polymorphisms (SNP); 1k-RiCA; Oryza sativa L.

Online: 25 May 2023 (08:58:09 CEST)

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Achieving high-yield potential is always the ultimate objective of any breeding program. However, various abiotic stresses like salinity, drought, cold, flood, and heat hampered rice productivity tremendously. Salinity is one of the most important abiotic stresses that adversely affect rice grain yield. The present investigation was undertaken to dissect new genetic loci, which are responsible for salt tolerance at the early seedling stage in rice. A bi-parental mapping population (F2:3) was developed from the cross between BRRI dhan28/Akundi, where BRRI dhan28 (BR28) is a salt-sensitive irrigated (boro) rice mega variety and Akundi is a highly salinity tolerant Bangladeshi origin indica rice landrace that is utilized as a donor parent. We report stable QTLs for salt tolerance from a common donor (Akundi) irrespective of two different genetic backgrounds (BRRI dhan49/Akundi and BRRI dhan28/Akundi). A robust 1k-Rice Custom Amplicon (1k-RiCA) SNP marker genotyping platform was used to genotype this bi-parental population. After eliminating markers with high segregation distortion, 886 polymorphic SNPs built a genetic linkage map covering 1526.5 cM of whole rice genome with an average SNP density of 1.72 cM for the 12 genetic linkage groups. A total of 12 QTLs for nine different salt tolerance-related traits were identified using QGene and inclusive composite interval mapping of additive and dominant QTL (ICIM-ADD) under salt stress on seven different chromosomes. All of these 12 new QTLs found unique, as no other map from the previous study has reported these QTLs in the similar chromosomal location and found them different from extensively studied Saltol, SKC1, OsSalT and salT locus. Twenty-eight significant digenic/epistatic interactions identified between chromosomal regions linked to or unlinked to QTLs. Akundi acts like a new alternate donor source of salt tolerance except for other usually known donors like Nona Bokra, Pokkali, Capsule, and Hasawi used in salt tolerance genetic analysis and breeding programs worldwide, including Bangladesh. Integration of the seven novel and stable QTLs (qSES1, qSL1, qRL1, qSUR1, qSL8, qK8, qK1) reported in this investigation will expedite the cultivar development that is highly tolerant to salt stress.

Preprint ARTICLE | doi:10.20944/preprints202305.1704.v1

Active Device for Shoulder Rehabilitation Based on Force Control

Isabel M. Alguacil-Diego, Alicia Cuesta-Gómez, David Pont-Esteban, Juan Carrillo-Rios, Paul Espinosa-Peralta, Miguel Ángel Sánchez-Urán, Manuel Ferré

Subject: Public Health And Healthcare, Physical Therapy, Sports Therapy And Rehabilitation Keywords: Force control; haptic; upper limb; rehabilitation; shoulder movements; medical rehabilitation

Online: 24 May 2023 (05:33:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1408.v1

Preliminary Exploration on the Low-Pressure Ar-O2 Plasma Generated by Low-Frequency Alternating Current (AC) Power Supply

Niaz Wali, W.W. Xiao, C.Y. Wang, J.T. Ma, W.J. Zhong

Subject: Physical Sciences, Fluids And Plasmas Physics Keywords: low-pressure Ar-O2 plasma; single Langmuir probe; electron density; electron temperature; elec-tron energy distribution function; low-frequency AC power supply

Online: 19 May 2023 (08:20:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1401.v1

Coarse and Fine Two-Stage Calibration Method for Enhancing the Accuracy of Inverse Finite Element Method

Jiewei Lu, Dahang He, Zhenyi Zhao, Hong Bao

Subject: Engineering, Mechanical Engineering Keywords: inverse finite element method; Shape sensing; Single-objective particle swarm optimization; Error correction model; Bayesian; Residual analysis; Fuzzy network

Online: 19 May 2023 (08:00:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1397.v1

Treating Cylindrically Populated Endometrial Cancers With High Dose Rate Vaginal Cuff Brachytherapy: Should We Use a MulTi-Channel or Single-Channel Cylinder?

Hualin Zhang, Andrew Lim, Zhuang Kang, Eric D Donnelly, Lijun Ma, Eric L. Chang

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: high dose rate brachytherapy; vaginal cuff brachytherapy; single channel cylinder; multiple channel cylinder, endometrial cancer.

Online: 19 May 2023 (07:42:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1365.v1

Lipid-Associated GWAS Loci Predict Antiatherogenic Effects of Rosuvastatin in Patients with Coronary Artery Disease

Stanislav Kononov, Iuliia Azarova, Elena Klyosova, Marina Bykanova, Mikhail Churnosov, Maria Solodilova, Alexey Polonikov

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: coronary artery disease; plasma lipids; carotid intima-media thickness; pharmacogenetics; personalized medicine; single nucleotide polymorphisms; lipid-lowering therapy; rosuvastatin.

Online: 19 May 2023 (03:26:56 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1282.v1

Investigation on Perceptual-Aware Optimization for Single Image Super Resolution on Embedded Systems

Khanh Hung Vu, Duc Phuc Nguyen, Duc Dung Nguyen, Hoang-Anh Pham

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: Single-Image Super-Resolution (SISR); Deep Learning; Quantization; Network Pruning; Knowledge Distillation

Online: 18 May 2023 (07:06:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1254.v1

The Association of Oxidative Stress-Related Gene Polymorphisms with Pain-Related Temporomandibular Disorders and Oral Behavioural Habits

Ema Vrbanović, Marko Zlendić, Koraljka Gall Trošelj, Marko Tomljanović, Kristina Vuković Đerfi, Iva Zilic-Alajbeg

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: antioxidants; chronic orofacial pain; oxidative-stress related genes; temporomandibular disorders; oral behavioural habits; single nucleotide polymorphism

Online: 17 May 2023 (14:15:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1171.v1

Topical Application of Autologous Peripheral Blood Mononuclear Cells in Patients with Small Artery Disease and Diabetic Foot Ulcers: Efficacy, Safety and Economic Evaluation

Benedetta Ragghianti, Bianca Maria Berardi, Edoardo Mannucci, Matteo Monami

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: Diabetes mellitus; Foot ulcer; Cell-therapy; Small Artery Disease; Chronic Limb-Threatening Ischemia; Economic evaluation

Online: 17 May 2023 (02:41:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1104.v1

Elucidating the Role of Immune Cell Dysregulation in Atypical Hemolytic Uremic Syndrome: A Comprehensive Single-Cell Sequencing Analysis

I-Ru Chen, Chiu-Ching Huang, Siang‑Jyun Tu, Guei-Jane Wang, Ping-Chin Lai, Ya-Ting Lee, Ju-Chen Yen, Ya-Sian Chang, Jan‑Gowth Chang

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: hemolytic uremic syndrome; complement; single cell sequencing

Online: 16 May 2023 (05:36:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0943.v1

Identification of Markers Associated with Wheat Dwarf Virus (WDV) Tolerance/Resistance in Barley (Hordeum vulgare ssp. vulgare) Using Genome Wide Association Studies

Behnaz Soleimani, Heike Lehnert, Sarah Trebing, Antje Habekuß, Frank Ordon, Andreas Stahl, Torsten Will

Subject: Biology And Life Sciences, Plant Sciences Keywords: barley; Hordeum vulgare ssp; vulgare; Wheat dwarf virus (WDV); leafhoppers; resistance; tolerance; single nucleotide polymorphisms (SNPs); marker; QTL

Online: 12 May 2023 (11:50:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0911.v1

Age Profiles for SARS-CoV-2 Variants in England and Wales and States of the USA (2020 to 2022): Impact on All-Cause Mor-Tality

Rodney P Jones, Andrey Ponomarenko

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID–19; all-cause mortality; single year of age; gender; complex system; pathogen interference; seasonality; small RNAs

Online: 12 May 2023 (09:01:29 CEST)

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Since 2020 COVID-19 has caused serious mortality around the world. Given the ambiguity in es-tablishing COVID-19 as the direct cause of death we first investigate the effects of age and sex upon all-cause mortality during 2020 and 2021 in England and Wales. Since infectious agents have their own unique age profile for death, we explore several methods to adjust single year of age deaths in England and Wales during 2019 (the pre-COVID-19 base year) to a pathogen-neutral single year of age baseline. This adjusted base year is then used to confirm the widely reported higher deaths in males for most ages above 43 years in both 2020 and 2021. During 2020 (+ COVID-19 but no vac-cination) both male and female population-adjusted deaths were significantly increased above age 35. A significant reduction in all-cause mortality among both males and females aged 75+ could be demonstrated in 2021 during widespread COVID–19 vaccination, however, below age 75 deaths progressively increased. This finding arises from a mix of vaccination and year of age profiles of deaths for the different SARS-CoV-2 variants. In addition, specific effects for age around puberty were demonstrated where females had higher deaths rather than males. There is evidence that year-of-birth cohorts may also be involved, indicating that immune priming to specific pathogen outbreaks in the past may lead to lower deaths for some birth cohorts. To specifically identify the age profile for the COVID-19 variants in 2020 to 2023, we employ the proportion of total deaths at each age which are potentially due to or ‘with’ COVID-19. The original Wuhan strain, and the Alpha variant show somewhat limited divergence in the age profile with the Alpha variant shifted to a moderately higher proportion of deaths below age 84. The Delta variant specifically targeted persons below age 65. The Omicron variants showed significantly lower proportion of overall mortality, with markedly higher relative proportion of deaths above age 65 steeply increasing with age to a maximum around 100 years of age. A similar age profile for the variants can be seen in the age-banded deaths in US states – although slightly obscured by using age bands rather than single year of age. However, the US data shows that higher male deaths are greatly dependent on age and the COVID-variant. Deaths determined as ‘due to’ COVID-19 (as opposed to ‘involving’ COVID-19) in England and Wales were especially over-estimated in 2021 relative to the change in all-cause mortality. This arose as a by-product of an increase in COVID-19 testing capacity in late 2020. Potential structure-function mechanisms for the year of age specificity of SARS-CoV-2 var-iants are discussed. The question is posed as to whether vaccines based on different variants carry the specific age profile through into the vaccine.

Preprint HYPOTHESIS | doi:10.20944/preprints202305.0607.v2

A Model That Reproduces an Empirical Rule Distinguishing the Developmental Path of Complete Hox Gene Clusters From Split Clusters

Spyros Papageorgiou

Subject: Biology And Life Sciences, Biophysics Keywords: Hox gene collinearity; temporal collinearity; Noether theory; self similarity; double strand break; split Ηox cluster; limb growth

Online: 11 May 2023 (04:34:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0648.v1

Genetic Characterization of Carbapenem-Resistant Klebsiella pneumoniae Clinical Isolates in a Tertiary Hospital in Greece, 2018-2022

Charalampos Zarras, Theodoros Karampatakis, Styliani Pappa, Elias Iosifidis, Eleni Vagdatli, Emmanuel Roilides, Anna Papa

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: carbapenem-resistant Klebsiella pneumoniae; whole genome sequencing; core genome single-nucleotide polymorphism analysis; molecular epidemiology

Online: 9 May 2023 (11:57:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0633.v1

Single-pass Process of Square Butt Joints without Edge Preparation using Hot-wire Gas Metal Arc Welding

Nattasak Suwannatee, Motomichi Yamamoto

Subject: Engineering, Industrial And Manufacturing Engineering Keywords: hot-wire; gas metal arc welding; single-pass welding; lower energy consumption welding

Online: 9 May 2023 (10:12:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0579.v1

Pneumatic Compression Combined with Standard Treatment after Total Hip Arthroplasty: Effects on Edema of the Operated Limb and on Physical Outcomes. A Clinical Randomized Control Trial

Vittoria Carnevale Pellino, Alessandro Gatti, Matteo Vandoni, Pamela Patanè, Massimiliano Febbi, Stefania Ballarin, Caterina Cavallo, Luca Marin

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: leg pneumatic compression; total hip arthroplasty; lower limb edema

Online: 9 May 2023 (05:44:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0530.v1

Characterization and Optimization of Multiomic Single-Cell Epigenomic Profiling

Leticia Sandoval, Wazim Mohammed Ismail, Amelia Mazzone, Mihai Dumbrava, Jenna Fernandez, Amik Munankarmy, Terra Lasho, Mortiz Binder, Vernadette Simon, Kwan Hyun Kim, Nicholas Chia, Jeong Heon Lee, S. John Weroha, Mrinal M. Patnaik, Alexandre Gaspar-Maia

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: single-cell sequencing; epigenomic profiling; snATAC-seq; snRNA-seq; nuclei preparation

Online: 8 May 2023 (11:00:52 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0503.v1

Emerging Quantitative Biochemical, Structural and Biophysical Methods to Study Ribosome and Protein-RNA Complex Assembly

Kavan Gor, Olivier Duss

Subject: Biology And Life Sciences, Biophysics Keywords: RNP assembly; ribosome assembly; protein-RNA interactions; RNA folding; assembly intermediates; in vitro reconstitutions; mass spectrometry; single-molecule fluorescence microscopy; cryo-electron microscopy; RNA structure probing

Online: 8 May 2023 (10:01:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0464.v1

Lipoic Acid-Functionalized Hexanuclear manganese(III) Nanomagnets Suitable for Surface Grafting

Marta Orts-Arroyo, Carlos Rojas-Dotti, Nicolás Moliner, José Martínez-Lillo

Subject: Chemistry And Materials Science, Inorganic And Nuclear Chemistry Keywords: manganese; lipoic acid; salicylamidoxime; crystal structure; magnetic susceptibility; single-molecule magnet

Online: 8 May 2023 (08:14:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0461.v1

Polymorphisms of the GCLC Gene Are Novel Genetic Markers For Susceptibility To Psoriasis Associated With Alcohol Abuse And Cigarette Smoking

Ekaterina Efanova, Olga Bushueva, Roman Saranyuk, Anna Surovtseva, Mikhail Churnosov, Maria Solodilova, Alexey Polonikov

Subject: Medicine And Pharmacology, Dermatology Keywords: Psoriasis; genetic susceptibility; oxidative stress; glutathione; glutamate cysteine ligase; GCLC; single nucleotide polymorphism; cigarette smoking; alcohol abuse; gene–environment interactions

Online: 8 May 2023 (08:03:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0164.v1

Laboratory Tests Using Distributed Fiber Optical Sensors to Validated a Future Field Application for Strain Monitoring Pile

Rodrigo Moraes Silveira, Marcelo Buras, André Luiz Delmondes Pereira Filho, Juliana Ferreira Fernandes, Marcos Massao Futai

Subject: Engineering, Civil Engineering Keywords: distributed fiber optics; tensile tests; single mode fiber

Online: 4 May 2023 (02:56:37 CEST)

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The use of fiber optics as a tool for different kinds of geotechnical monitoring can become highly attractive and cost effective when compared to conventional instruments such as piezometers, inclinometers, among others. A single fiber optic cable may cover a larger monitoring area compared to conventional instrumentation, and the possibility of monitoring more than one physical quantity with the same fiber optic cable. Consulting the literature, it is possible to find several different examples where distributed fiber optic systems are being used. For the use of any sensor, a calibration curve is required. In the case of strain sensors, calibration is required to derive strain values from the frequency measurement quantity. However, fiber optic sensor cable manufacturers often do not provide their own calibration parameters and consult the values in specialized literature, which can result in monitoring errors. In this context, this article presents a bench adjusted for tests on single mode fiber optic cables, as well as results of tensile tests aimed at defining the function of strain variations of two different optical fiber cables, manufactured by different companies, using two different distributed interrogators. This paper also proposes a methodology for calibrating of the fiber optic cables deformation since the calibration parameters are provided by the cable manufacturers, which can result in errors depending on the application. It should be noted that there are few manufacturers of fiber optic cables aimed at application in civil engineering. Therefore, with the calibration methodology proposed in this paper, it is intended to transmit to the academic community the possibility of obtaining calibration parameters of any fiber optic cable, even those manufactured for telecommunications purposes and not only for cables manufactured with the intended use in civil engineering. Because of this fact, researchers will not be restricted to the acquisition of special cables for their applications. Based on the results, it was possible to conclude that the application of calibrated fiber optic sensors in experimental piles foundations is viable, in order to evaluate the load-displacement behavior of these elements, under different loading conditions.

Preprint REVIEW | doi:10.20944/preprints202305.0151.v1

Epididymis May Be More Important in Male Fertility than Our Cognition

Meng-Meng Liu, Hao Shang, Xin-Lei Feng, Chao Qi, Shu-Er Zhang, Yuan-Chao Sun, Chun-Yang Gou, Yu-Jiang Sun, Guo-Liang Zhang

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Epididymis; Regional; Single cell/Spatial Transcriptomics

Online: 3 May 2023 (12:43:28 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.1013.v1

Solid-Phase Optical Sensing Techniques for Sensitive Virus Detection

Elif Seymour, Fulya Ekiz Kanik, Sinem Diken Gur, MONIREH Bakhshpour-Yucel, Ali Araz, Nese Lortlar Unlu, M. Selim Ünlü

Subject: Engineering, Electrical And Electronic Engineering Keywords: Solid-phase optical biosensors; virus diagnostics; fluorescence-based sensors; surface plasmon resonance; optical resonators; interferometric biosensors; single virus detection

Online: 27 April 2023 (03:25:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0965.v1

Luminescence Properties of Epitaxial Cu₂O Thin Films Electrodeposited on Metallic Substrates and Cu₂O Single Crystals

Laima Trinkler, Dajin Dai, Liuwen Chang, Mitch Ming Chi Chou, Tzu-Ying Wu, Jevgenijs Gabrusenoks, Dace Nilova, Rihards Ruska, Baiba Berzina, Ramunas Nedzinskas

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: cuprous oxide; single crystal; epitaxial thin films; electrodeposition; photoluminescence; optical absorption.

Online: 26 April 2023 (07:27:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0773.v1

Identification and Functional Analysis of Transcriptome Profiles, Long Non-coding RNAs, Single-Nucleotide Polymorphisms, and Alternative Splicing from the Oocyte to the Preimplantation Stage of Sheep by Single-Cell RNA Sequencing

Zi Jing Zhang, Qiao ting Shi, Xiao ting Zhu, Lei Jin, Li min Lang, Shi jie Lyv, Xiao ling Xin, Yong zhen Huang, Xiang Yu, Zhi ming Li, Su Juan Chen, Zhao xue Xu, Wei Zhang, Er yao Wang

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Key words: transcriptome profiles; long non-coding RNAs; single-nucleotide polymorphisms; alternative splicing; sheep; preimplantation; Single-cell RNA sequencing

Online: 23 April 2023 (04:57:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0148.v1

Hyperchaotic Maps and the Single Neuron Model: A Novel Framework for Chaos-Based Image Encryption

Wassim Alexan, Yen-Lin Chen, Lip Yee Por, Mohamed Gabr

Subject: Computer Science And Mathematics, Security Systems Keywords: Cryptography; Hyperchaotic maps; Image encryption; NIST; S-box; Single neuron model.

Online: 10 April 2023 (04:57:30 CEST)

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Preprint REVIEW | doi:10.20944/preprints202303.0371.v1

Nuclei on the Rise: When Nuclei-Based Methods Meet Next-Generation Sequencing

Tamer Butto, Kanak Mungikar, Peter Baumann, Jennifer Winter, Beat Lutz, Susanne Gerber

Subject: Medicine And Pharmacology, Hematology Keywords: Nuclei isolation; Next-generation sequencing; Cell-type specific isolation; Epigenetics; Transcriptomics; Single cell sequencing; Single nucleus sequencing

Online: 21 March 2023 (06:40:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202303.0362.v1

First-Principle Prediction of Stress-Tunable Single-Photon Emitters at Telecommunication Band from Point Defects in GaN

Junxiao Yuan, Ke Wang, Yidong Hou, Feiliang Chen, Qian Li

Subject: Physical Sciences, Optics And Photonics Keywords: single-photon emitters; atom defect; first principle calculations; telecommunication band; stress

Online: 21 March 2023 (01:44:51 CET)

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Preprint REVIEW | doi:10.20944/preprints202303.0361.v1

Inconsistent Outcomes of Metabolic Surgery for Nonobese Patients with Type 2 Diabetes

Kyung Yul Hur

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: inconsistent outcomes; epithelial-mesenchymal crosstalk; altered identity; duodenal-jejunal bypass; biliopancreatic limb; GIP

Online: 21 March 2023 (01:36:35 CET)

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Preprint ARTICLE | doi:10.20944/preprints202303.0103.v1

The Expected Competitive Ratio on a Kind of Stochastic-Online Followtime Scheduling with Machine Subject to an Uncertain Breakdown

Zhenpeng Li, Congdian Cheng

Subject: Computer Science And Mathematics, Applied Mathematics Keywords: single machine; single breakdown; schedule; flowtime; stochastic optimization; online optimization

Online: 6 March 2023 (13:00:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0377.v1

Employment of Fracture Mechanics Criteria for Accurate Assessment of the Full Set of Elastic Constants of Orthorhombic/Tetragonal Mono-Crystalline YBCO

Reaz A. Chaudhuri

Subject: Engineering, Mechanical Engineering Keywords: Three-dimensional Stress Singularity; Fracture Mechanics Criterion; Sufficient Condition for Fracture; Easy Cleavage System; Orthorhombic Single Crystal; Elastic Constants of Mono-crystalline YBCO

Online: 22 February 2023 (07:00:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0346.v1

Tightly Coupled INS/APS Passive Single Beacon Navigation

Zhuoyang Zou, Wenrui Wang, Bin Wu, Lingyun Ye, Washington Yotto Ochieng

Subject: Engineering, Marine Engineering Keywords: INS/APS single beacon navigation; tightly coupled navigation; passive synthetic aperture; joint DOA/OWTT estimation

Online: 21 February 2023 (01:56:42 CET)

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Preprint REVIEW | doi:10.20944/preprints202301.0323.v1

An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations

Morgan MacKenzie, Christos Argyropoulos

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: next-generation sequencing; nanopore sequencing; biosensors; single-molecule analysis; molecular diagnostics; genetics; transcriptomics; epigenetics

Online: 18 January 2023 (07:36:43 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0476.v1

Optimization of a Single Ion Heat Engine for Adiabatic Processes at High and Low Temperature Limits

Abdi Belina Atomsa, Tolasa Adugna Dima

Subject: Physical Sciences, Applied Physics Keywords: Adiabatic; figure of merit; Heat engine; Single

Online: 26 December 2022 (06:50:12 CET)

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Preprint REVIEW | doi:10.20944/preprints202212.0424.v1

The Value of N-of-1 Data in Zoological Medicine: A Methodological Review

Charles O. Cummings, Jessica Eisenbarth, David D.R. Krucik

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: systematic review; meta-analysis; evidence synthesis; RCT; N-of-1 trial; single subject design

Online: 22 December 2022 (07:56:51 CET)

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N-of-1 data are unavoidable in zoological medicine. Accordingly, zoological medicine clinicians and investigators need research techniques that can make use of these data. This article reviews two methodologies for using both observational and experimental N-of-1 data: 1) systematic reviews and meta-analyses of case reports and 2) prospective N-of-1 trials. Systematic reviews of case reports and other observational evidence are formal, unbiased summaries of clinical characteristics of a particular disease-taxon combination. They offer advantages to narrative reviews by minimizing omission of relevant articles, thereby reducing the potential for mischaracterization of the literature. Meta-analyses are extensions of systematic reviews that quantitatively synthesize the data from the included articles. While valuable, systematic reviews and meta-analyses of case reports can have limited interpretations due to publication bias and confounding present in their source materials. In contrast to case reports, N-of-1 trials are prospective study designs that allow clinicians to make strong inferences about the effect of an intervention in a particular patient. They are double-blinded, single patient, multi-crossover studies that are of particular value in fields where it is difficult to recruit sufficient patients for conventional randomized control trials (RCTs), such as zoological medicine. Because they require multiple crossover periods, N-of-1 trials are ideal for evaluating short-acting interventions in patients with somewhat stable chronic diseases, such as osteoarthritis. More complex than conventional therapeutic trials, N-of-1 trials require prior consideration of how to achieve blinding, appropriate placebo controls, quantitative primary outcomes, analysis methods, and ethical approval. Aggregation of N-of-1 trials allows estimation of the average treatment effect across the population with fewer participants than a conventional RCT. While systematic reviews and meta-analyses of case reports can be used to synthesize the observational N-of-1 data already in existence, N-of-1 trials offer an exciting way to prospectively generate strong evidence that will be useful for evidence-based decision-making.

Preprint ARTICLE | doi:10.20944/preprints202212.0319.v1

HERTZIAN MOTOR: An Innovative Method to Obtain an Energy Efficiency of 90%, in Savings in Single-Phase Active Energy (Kwh), If The “Fan Law” Is Applied To PSMSM-Type Synchronous Motors Without The Need to Apply The Use of Variable Frequency Drives (VFD)

Ibar Anderson

Subject: Engineering, Electrical And Electronic Engineering Keywords: Energy efficiency; single-phase active energy; kWh; electricity saving; Fan Affinity Law

Online: 19 December 2022 (04:02:27 CET)

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This paper proposes how to reduce the consumption of active electrical energy (kwh) by 90.3% while maintaining the same mechanical work speed (RPM) at variable torque loads (fans or air-fluid blowers, centrifugal pumps are not included). of water and similar fluids), by using the “Fan Law” in an innovative way in PMSM-type synchronous motors (a comparative study never before carried out on the Fan Law). The work is carried out comparatively between brushless a-synchronous motors with starting loop (or motor with a short-circuited loop) versus brushed a-synchronous motors and PMSM-type synchronous motors without the need to use VDF (variable frequency drives), simplifying technology (electronics) and saving costs in an innovative way (R+D+i). The case study was developed on a design applied to a centrifugal air extractor/blower with PMSM/IPM type synchronous motor. Applying one of the fan affinity laws –with the impeller diameter 10.5 (mm) constant- the electrical power absorbed by the blower motor is proportional to the cube of the shaft speed: . Being “P” power (Watts) and “N” speed (RPM). Carrying out a comparative study between power (watts), active energy consumption (kwh) and rotational speed (RPM). This has a direct impact on the costs of residential and commercial single-phase active electrical energy consumption, measured in kilowatt-hours (kwh). Carrying out a comparative study between three (3) types of alternating current (AC) electrical machines, according to the NEMA (National Electrical Manufacturers Association); AC motors fall into three (3) categories. One (1), synchronous motors (Syncrhonous Motor) of three types: (1a) excitation by DC (DC Excited Motor), (1b) permanent magnet (Permanent Magnet Motor) and (1c) reluctance motor (Reluctance Motor) or motor Step by Step. Two (2), asynchronous induction motors of two types: (2a) Squirrel-Cage Induction Motor and (2b) Wound-Rotor Induction Motor. Three (3) series-wound motor (Series-Wound Motor) also called universal motor (they have carbons).

Preprint ARTICLE | doi:10.20944/preprints202212.0152.v1

Eugenio Martelli, Matilde Zamboni, Giovanni Sotgiu, Laura Saderi, Massimo Federici, Giuseppe Massimo Sangiorgi, Mariangela Valentina Puci, Allegra Rosa Martelli, Teresa Messina, Paolo Frigatti, Maria Pia Borrelli, Carlo Ruotolo, Ilaria Ficarelli, Paolo Rubino, Francesco Pezzo, Luciano Carbonari, Andrea Angelini, Edoardo Galeazzi, Luca Calia Di Pinto, Franco Michelino Fiore, Armado Palmieri, Giorgio Ventoruzzo, Giulia Mazzitelli, Franco Ragni, Antonio Bozzani, Enzo Forliti, Claudio Castagno, Pietro Volpe, Mafalda Massara, Diego Moniaci, Elisa Pagliasso, Tania Peretti, Mauro Ferrari, Nicola Troisi, Pietro Modugno, Maurizio Maiorano, Umberto Marcello Bracale, Marco Panagrosso, Mario Monaco, Giovanni Giordano, Giuseppe Natalicchio, Antonella Biello, Giovanni Maria Celoria, Alessio Amico, Mauro Di Bartolo, Massimiliano Martelli, Roberta Munaó, Davide Razzano, Giovanni Colacchio, Francesco Bussetti, Gaetano Lanza, Antonio Cardini, Bartolomeo Di Benedetto, Mario De Laurentis, Maurizio Taurino, Pasqualino Sirignano, Pierluigi Cappiello, Andrea Esposito, Santi Trimarchi, Silvia Romagnoli, Andrea Padricelli, Giorgio Giudice, Adolfo Crinisio, Giovanni Di Nardo, Giuseppe Battaglia, Rosario Tringale, Salvatore De Vivo, Rita Compagna, Valerio Stefano Tolva, Ilenia D'Alessio, Ruggiero Curci, Simona Giovannetti, Giuseppe D'Arrigo, Giusi Basile, Dalmazio Frigerio, Gian Franco Veraldi, Luca Mezzetto, Arnaldo Ippoliti, Fabio Massimo Oddi, Alberto Maria Settembrini

Subject: Medicine And Pharmacology, Hematology Keywords: chronic limb-threatening ischemia; outcome; sex; age; limb salvage

Online: 8 December 2022 (09:39:30 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0065.v1

Comparative Genetic Association Analysis of Human Genetic Susceptibility to Pulmonary and Lymph Node Tuberculosis

Abhimanyu -, Mridula Bose, Astha Giri, Mandira Varma-Basil

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: pulmonary tuberculosis; lymph node tuberculosis; extra-pulmonary tuberculosis; single nucleotide polymorphisms; cytokine; innate immunity; genetic association; genotype; serum

Online: 5 December 2022 (08:00:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0475.v1

The Local Coordination Effects on the Reactivity and Speciation of Active Sites in Graphene-Embedded Single-Atom Catalysts Over Wide pH and Potential Range

Milica S. Ritopečki, Ana S. Dobrota, Natalia V. Skorodumova, Igor A. Pašti

Subject: Chemistry And Materials Science, Nanotechnology Keywords: single-atom catalysts; graphene; activity; reactivity; stability; Pourbaix plots

Online: 25 November 2022 (10:06:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0307.v1

Energy Efficient Centrifugal Air Extractor for Environments Contaminated With Sars-Cov-2 (Coronavirus).How to Build a Motor That Saves Electricity

Ibar Federico Anderson

Subject: Engineering, Mechanical Engineering Keywords: Centrifugal fan; SARS-CoV-2; COVID-19; energy efficiency; synchronous motor; PMSM/IPM; single-phase alternating current

Online: 16 November 2022 (12:43:05 CET)

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The product is a centrifugal air extractor, whose objective is to extract the stale air of SARS-CoV-2 or Covid-19 (Coronavirus); designed for civil and commercial use, it works with a single-phase alternating current (AC) motor of 220 (V) and 50 (Hz), with high energy efficiency (EE). Developed under the Design Thinking methodology, by electromechanical simulation using NI Multisim 14.0 software, case design using Cfturbo 2020 R2.0 CAD software, and rapid 3D prototyping with the OverLord Pro printer; with a prototype of the conventional stator winding of a synchronous motor with a two-pole PMSM/IPM single-phase alternating current (AC) field winding and a 4000 (Gauss) ferromagnetic ceramic magnet rotor. Innovating online nº 15 of invention patent nº 381968 by Nikola Tesla, 5/1/1888.One of the objectives is to link the middle level or Technical Schools with the University system (and careers with a Technological profile) through Project B374 located in the Secretariat of Science and Technology (SCyT) of the National University of La Plata ( UNLP), whose title is: “Integrated Management of Design and Innovation. Contributions for a theoretical-conceptual and methodological review” by the Director: Mg. Federico del Giorgio Solfa. Just as links were sought with the Research and Development Laboratory of the Department of Industrial Design (LIDDI-FBA-UNLP) through its Director D.I. Pablo Ungaro and D.I. Ana Bocos. In 2021, the project participated in the INNOVAR National Contest of the Ministry of Science, Technology and Productive Innovation of the Nation (MINCYT), Argentine Republic; in the "Covid-19 Projects" category, having been selected in the catalog of innovative products, won the 1st. "Covid-19 Projects" position of the National Agency for Research and Technological Development and local innovation (R+D+i) dependent on MINCYT.The results showed, according to the analysis of the test bench, that the PMSM/IPM type synchronous motor used in the centrifugal fan, with the innovation of the reactance-inductive control in series plus the capacitor in parallel, reduces the power by 67% (Watts) and active energy consumption (kWh), performing 56% more mechanical work (Joules) on the fluid air (with a 50% reduction in carbon footprint). Which leads us to the following conclusion: it is possible to develop centrifugal fans that save electricity (kWh) without the need to resort to (1) the "Fan Affinity Law", nor (2) the use of variable speed drives (VDF) or frequency (which are devices with complex and expensive electronics). Which would bring an enormous saving of the cost of electrical energy.

Preprint ARTICLE | doi:10.20944/preprints202211.0180.v1

Measuring Democratic Voice

Denis Falvey

Subject: Social Sciences, Political Science Keywords: democracy; electoral systems; ballot split by type; legislative empowerment measure (LEM); ac-countable local representation measure (ALRM); single member district proportional representation (SMDPR)

Online: 10 November 2022 (01:15:52 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0129.v1

Gradient-Applied Weighted Loss for Details of 3D Shape in Single-View Reconstruction

Jiho Lee, Taehyeon Kim, Gihwan Lee, Yoonsik Choe

Subject: Engineering, Electrical And Electronic Engineering Keywords: 3D shapes; single-view reconstruction; gradient map; fine-grained

Online: 8 November 2022 (01:04:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202210.0396.v1

Feasibility Study of Cable Routing for Bi-Planar Trajectory Tracking in Cable Driven Lower Limb Exoskeleton

Rajan Prasad, Marwan El-Rich, Mohammad I. Awad, Sunil K. Agrawal, Kinda Khalaf

Subject: Engineering, Mechanical Engineering Keywords: cable driven; exoskeleton; lower limb rehabilitation; hip adduction; bi-planar trajectory; optimized routing.

Online: 26 October 2022 (03:54:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0358.v1

A Modified single frequency PPP method for the Positioning and Time transfer with BDS-3

Mingjun OuYang, Xiangwei Zhu, Junzhi Li, Liu Yang

Subject: Environmental And Earth Sciences, Space And Planetary Science Keywords: BDS-3; Time transfer; Precise point positioning; Single frequency; Group and Phase Ionospheric Correction (GRAPHIC)

Online: 24 October 2022 (10:01:41 CEST)

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