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A Flexible and Transparent PtNP/Swcnt/Pet Electrochemical Sensor for Nonenzymatic Detection of Hydrogen Peroxide Released From Living Cells With Real-time Monitoring Capability
Oh, D.E.; Lee, C.-S.; Kim, T.W.; Jeon, S.; Kim, T.H. A Flexible and Transparent PtNP/SWCNT/PET Electrochemical Sensor for Nonenzymatic Detection of Hydrogen Peroxide Released from Living Cells with Real-Time Monitoring Capability. Biosensors2023, 13, 704.
Oh, D.E.; Lee, C.-S.; Kim, T.W.; Jeon, S.; Kim, T.H. A Flexible and Transparent PtNP/SWCNT/PET Electrochemical Sensor for Nonenzymatic Detection of Hydrogen Peroxide Released from Living Cells with Real-Time Monitoring Capability. Biosensors 2023, 13, 704.
Oh, D.E.; Lee, C.-S.; Kim, T.W.; Jeon, S.; Kim, T.H. A Flexible and Transparent PtNP/SWCNT/PET Electrochemical Sensor for Nonenzymatic Detection of Hydrogen Peroxide Released from Living Cells with Real-Time Monitoring Capability. Biosensors2023, 13, 704.
Oh, D.E.; Lee, C.-S.; Kim, T.W.; Jeon, S.; Kim, T.H. A Flexible and Transparent PtNP/SWCNT/PET Electrochemical Sensor for Nonenzymatic Detection of Hydrogen Peroxide Released from Living Cells with Real-Time Monitoring Capability. Biosensors 2023, 13, 704.
Abstract
We developed a transparent and flexible electrochemical sensor using a platform based on a network of single-walled carbon nanotubes (SWCNTs) for the non-enzymatic detection of hydrogen peroxide (H2O2) released from living cells. We decorated the SWCNT network on a poly(ethylene terephthalate) (PET) substrate with platinum nanoparticles (PtNPs) using a potentiodynamic method. The PtNP/SWCNT/PET sensor synergized the advantages of a flexible PET substrate, a conducting SWCNT network, and a catalytic PtNP, and demonstrated good biocompatibility and flexibility, enabling cell adhesion. The PtNP/SWCNT/PET-based sensor demonstrated enhanced electrocatalytic activity towards H2O2 as well as excellent selectivity, stability, and reproducibility. The sensor exhibited a wide dynamic range of 500 nM to 1 M, with a low detection limit of 228 nM. In addition, Furthermore, the PtNP/SWCNT/PET sensor remained operationally stable even after bending at various angles (15°, 30°, 60°, and 90°) with no noticeable loss of current signal. These outstanding characteristics enabled the PtNP/SWCNT/PET sensor to be practically applied for the direct culture of HeLa cells and the real-time monitoring of H2O2 release by the HeLa cells under drug stimulation.
Chemistry and Materials Science, Analytical Chemistry
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