Version 1
: Received: 22 June 2023 / Approved: 26 June 2023 / Online: 26 June 2023 (11:01:46 CEST)
Version 2
: Received: 28 July 2023 / Approved: 28 July 2023 / Online: 31 July 2023 (09:42:16 CEST)
Huang, L.; Tang, J.; Zhu, B.; Chen, G.; Chen, L.; Bu, S.; Zhu, H.; Liu, Z.; Li, Z.; Meng, L.; et al. QTL Epistasis Plays a Role of Homeostasis on Heading Date in Rice. Scientific Reports 2024, 14, doi:10.1038/s41598-023-50786-x.
Huang, L.; Tang, J.; Zhu, B.; Chen, G.; Chen, L.; Bu, S.; Zhu, H.; Liu, Z.; Li, Z.; Meng, L.; et al. QTL Epistasis Plays a Role of Homeostasis on Heading Date in Rice. Scientific Reports 2024, 14, doi:10.1038/s41598-023-50786-x.
Huang, L.; Tang, J.; Zhu, B.; Chen, G.; Chen, L.; Bu, S.; Zhu, H.; Liu, Z.; Li, Z.; Meng, L.; et al. QTL Epistasis Plays a Role of Homeostasis on Heading Date in Rice. Scientific Reports 2024, 14, doi:10.1038/s41598-023-50786-x.
Huang, L.; Tang, J.; Zhu, B.; Chen, G.; Chen, L.; Bu, S.; Zhu, H.; Liu, Z.; Li, Z.; Meng, L.; et al. QTL Epistasis Plays a Role of Homeostasis on Heading Date in Rice. Scientific Reports 2024, 14, doi:10.1038/s41598-023-50786-x.
Abstract
To avoid the endless accumulation of QTL effects during pyramiding breeding, QTL epistasis plays a role of homeostasis. Four single-segment substitution lines with heading date QTLs were utilized to construct pyramiding materials with dual QTLs and triple QTLs, allowing for the estimation of QTL epistatic effects on heading date in rice. The epistatic components of dual QTL and triple QTL interactions were analyzed using data from three seasons of two years. All four QTLs can be considered as dominant loci, demonstrating superdominance. 75% of dual QTL interactions and 81.25% of triple QTL interactions obtained statistically significant results, reaffirming the prevalence of epistasis. Interactions of four QTLs with three positive effects and one negative effect generated 62.5% negative dual QTL epistatic effects and 57.7% positive triple QTL epistatic effects, forming the balance relationship “positive-negative-positive”. In fact, QTL effects were consistently partially neutralized by mixed QTL epistatic effects so as to control the infinite accumulation of QTL effects. QTL epistasis plays a role of homeostasis on heading date in rice. However, there were two exceptions. The directions of OsMADS50 and Hd3a-2 effects were consistently aligned with those of their mixed epistases, suggesting that the QTL or gene could be employed in pyramiding breeding with different objectives. This study elucidated the mechanism of epistatic interactions among four QTLs and provided valuable genetic resources for improving heading date in rice.
Keywords
Epistasis; Homeostasis; Single segment substitution line; Heading date; Rice
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
