MacKenzie, M.; Argyropoulos, C. An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations. Micromachines2023, 14, 459.
MacKenzie, M.; Argyropoulos, C. An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations. Micromachines 2023, 14, 459.
MacKenzie, M.; Argyropoulos, C. An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations. Micromachines2023, 14, 459.
MacKenzie, M.; Argyropoulos, C. An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations. Micromachines 2023, 14, 459.
Abstract
There have been several developments in the field of nanopore biosensor development and sequencing applications that address previous limitations that restricted widespread nanopore use. These innovations, paired with the large-scale commercialization of biological nanopore sequencing by Oxford Nanopore Technologies, are making these platforms a mainstay in contemporary research labs. Equipped with the ability to provide long and short-read sequencing information with quick turn-around times and simple sample preparation, nanopore sequencers are quickly improving our understanding of unsolved genetic, transcriptomic, and epigenetic problems. However, there remain some key obstacles that have yet to be improved. In this review, we provide a general introduction to nanopore sequencing principles, discussing biological and solid-state nanopore developments, obstacles to single-base detection, and library preparation considerations. We conclude with examples of important clinical applications to give perspective on the potential future of nanopore sequencing in the field of molecular diagnostics.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
