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Preprint REVIEW | doi:10.20944/preprints202404.0841.v1

Review of Taxonomic Knowledge of Dictyota (Phaeophyceae; Dictyotales); Two Centuries after its Description

María del Carmen Altamirano-Cerecedo, Francisco Omar López-Fuerte, Juan Manuel López-Vivas, Elisa Serviere-Zaragoza, Karla León-Cisneros

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: alpha taxonomy; beta taxonomy; gamma taxonomy; macroalgae; integrative taxonomy

Online: 12 April 2024 (10:08:36 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202403.1253.v1

Gas Gangrene in a Horse in the Amazon Biome Caused by Clostridium septicum

Loise Araújo de Sousa, Leandro Oliveira da Silva, Márcio Alan Oliveira Moura, Marcos Dutra Duarte, Valíria Duarte Cerqueira, Rodrigo Otávio Silveira Silva, Felipe Masiero Salvarani

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Clostridium septicum, alpha toxin, multiplex PCR, malignant edema, claudication.

Online: 21 March 2024 (10:46:02 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202402.0189.v1

Examining TNF-Alpha Inhibition Potential: Molecular Docking Study of Pharmaceuticals and Natural Compounds

Ivan Vito Ferrari

Subject: Public Health And Healthcare, Other Keywords: autodock vina; pyrx program; TNF-alpha; inflammation; docking studies

Online: 5 February 2024 (06:24:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.1698.v2

Mapping of Alpha-Emitting Radionuclides in the Environment Using an Unmanned Aircraft System

Pablo Royo, Arturo Vargas, Tania Guillot, David Saiz, Jonathan Pichel, Daniel Rabago, María Amor Duch, Claudia Grossi, Maksym Luchkov, Volker Dangendorf, Faton Krasniqi

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: radiological detection; alpha-emitting radionuclides; UAS software architecture; UAS

Online: 21 December 2023 (16:02:51 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202312.1085.v1

TNF-α Inhibitors Rescue APS-Induced Dangerous Pregnancies by Reducing the Risk of Miscarriage: A Report of 7 Cases

Ran An, Xiaolei Wang, Ligui Xiong, Yanqi Yang, Peiling Li

Subject: Medicine And Pharmacology, Obstetrics And Gynaecology Keywords: Antiphospholipid syndrome; TNF-α Inhibitors; Dangerous pregnancy; gestational weeks

Online: 14 December 2023 (12:15:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.2133.v1

Structural Evolution of Primate Glutamate Dehydrogenase 2 as Revealed by in Silico Predictions and Experimentally Determined Structures

Ionela Litso, Andreas Plaitakis, Vasiliki E. Fadouloglou, Mary Providaki, Michael Kokkinidis, Ioannis Zaganas

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: glutamate dehydrogenase; alpha fold; protein structure prediction; primate evolution

Online: 30 May 2023 (11:55:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202301.0202.v1

Behavioural and Neurophysiological Assessment of Hearing Aid Benefits in an Adult Population with and without Hearing Loss

Shivali Appaiah-Konganda, Mridula Sharma, Ronny Ibrahim, Jessica J. M. Monaghan, John Newall, Joaquin Tomas Valderrama Valenzuela

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Alpha synchronisation; Working memory; Auditory processing; Modulation detection threshold

Online: 12 January 2023 (01:48:00 CET)

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Background: Despite hearing aids adequately compensate for hearing loss, a substantial proportion of the population leave their hearing difficulties untreated. Even though this is a well-known clinical issue, the optimal approach to address this issue during the hearing rehabilitation process is still unclear. Purpose: The present study aims to characterise behavioural and neurophysiological auditory and cognitive processing skills in experienced hearing aid users versus those with normal hearing, aimed at providing clinicians with the evidence required to adequately manage the expectations of their clients, thus indirectly reinforcing hearing-aid adoption within the adult population with hearing loss. Research design: Behavioural tests included auditory, cognitive, and speech-in-noise tasks; and neurophysiological testing included cortical auditory evoked potentials evoked by a /da/ stimulus presented at 65 dB SPL. The tests were selected based on previous literature supporting specific speech-understanding skills. Study sample: Ten participants (7 female, 21—68 years) with bilateral, mild-moderate to moderately-severe sensorineural hearing loss (HL), and 10 with clinically normal hearing (NH, 8 female, 19—62 years) participated in the study. Data collection and analysis: Behavioural data was analysed using multivariate analysis of variance (MANOVA) and neurophysiological analysis used measurements such as independent t-test, time-frequency analysis and inter-trial phase coherence. Results: The NH and HL groups presented similar scores in all the behavioural tasks. Time-frequency analysis revealed a statistically significantly reduction in alpha (8–12 Hz) synchronisation at the centro-frontal electrodes in the HL group – a brain activity pattern that has been associated with listening effort, inhibition and selective attention. Conclusions and significance: Results support the conclusion that hearing aids are effective in compensating for the audibility of their users, enabling them to perform at similar levels than their normal-hearing peers. However, the reduced alpha synchronisation observed in the HL population indicates that adequate audibility does not extend to improved neural responses. Future studies need to investigate the induced activity in speech understanding paradigms to explore the auditory processing differences at cortical level. The results are only from a small sample size but the findings have the potential to support clinicians in managing adequately the expectations of their clients in regards the benefits of hearing aid technologies.

Preprint ARTICLE | doi:10.20944/preprints202206.0274.v1

α-amylase Inhibition Activity of Phytoconstituents Present in the Roots Of Cyclea peltata-An in-silico and in-vitro Investigation

Gandhimathi R., Binoy Varghese Cheriyan, Deevan Paul A., Saravanakumar A., Lakshmanan G.

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Cyclea peltata; Diabetes Mellitus; Docking; Schrodinger; Alpha amylase; Inhibition

Online: 20 June 2022 (14:33:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202102.0472.v1

Potential of Entomopathogenic Nematodes versus Alpha-cypermethrin Against Potato Tuber Moth, Phthorimaea operculella Zeller 1873 (Lep.: Gelechiidae) in Storage Conditions

Laleh Ebrahimi, Aziz Sheikhigarjan, Mehran Ghazavi

Subject: Biology And Life Sciences, Insect Science Keywords: EPNs; PTM; biological control; potato tuber; efficacy; alpha-cypermethrin

Online: 22 February 2021 (13:19:25 CET)

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Preprint ARTICLE | doi:10.20944/preprints202011.0428.v1

On the Fourth Coefficient of the Inverse of a Starlike Function of Positive Order

Toshiyuki Sugawa, Li-Mei Wang

Subject: Computer Science And Mathematics, Algebra And Number Theory Keywords: starlike function of order $\alpha$; inverse function; coefficient estimates

Online: 16 November 2020 (15:08:35 CET)

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Preprint ARTICLE | doi:10.20944/preprints201909.0130.v1

Synthesis of novel Triazinoindole-Based-Thiourea Hybrid: α-Glucosidase Inhibitors and Their Molecular Docking Study

Muhammad Taha, Foziah J. Alshamrani, Fazal Rahim, Shawkat Hayat, Hayat Ullah, Khalid Zaman, Syahrul Imran

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: Synthesis; triazinoindole; thiosemicarbazide; alpha-glucosidase; molecular docking study; SAR

Online: 13 September 2019 (10:54:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201811.0324.v1

Associations between the Duration and Frequency of Sedentary Bouts and Adiposity in Elderly Community-Dwelling Women

Jana Pechová, Jana Pelclová, Aleš Gába, Izabela Zając-Gawlak, Lenka Tlučáková, Jan Dygrýn, Zuzana Svozilová, Hana Lepková

Subject: Social Sciences, Behavior Sciences Keywords: Adipose tissue, Adiposity, Sedentary bouts, Alpha sedentary, Elderly women

Online: 14 November 2018 (08:09:04 CET)

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Preprint REVIEW | doi:10.20944/preprints202005.0293.v1

Microgravity, Bone Homeostasis, and Insulin-like Growth Factor-1

John Kelly Smith

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Insulin-like growth factor-1; Insulin-like growth factor-1 receptor; microgravity; osteoblasts; osteocytes; osteoclasts; IGF-1; IGF1R; rIGF-1

Online: 18 May 2020 (03:31:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0648.v1

Regulation of Krüppel-like Factor 15 Expression by Herpes Simplex Virus Type 1 or Bovine Herpesvirus 1 Productive Infection

Fouad S. El-Mayet, Kelly S. Harrison, Clinton Jones

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: herpes simplex virus type 1 (HSV-1); bovine herpesvirus 1 (BoHV-1); Krüppel–like factor 15 (KLF15); infected cell protein 0 (ICP0); BoHV-1 ICP0 (bICP0)

Online: 26 May 2021 (15:10:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0111.v2

Angiopoietins as Predictor Indexes in COVID-19 Patients in Delta and Omicron Waves

Panagiota Tsiatsiou, Kyriakos Kouirouxis, Vasiliki Tsaireli, Antonia Lanta, Angeliki Kassomenaki, Maria Papaioannou, Efthymia Protonotariou, Lemonia Skoura

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: Angiopoietin-1 (Ang-1); Angiopoietin-2 (Ang-2); Angiopoietin-2/ Angiopoietin-1 ratio; (Ang-2/Ang-1); COVID-19; endothelial dysfunction

Online: 21 February 2024 (04:26:18 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.0005.v1

A mini review on glucagon-like peptide-1 receptor agonists and its role in blood pressure regulation

Nora H. J Alharbi

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Agonist, blood pressure, GLP-1, GLP-1 receptor, hypertension

Online: 1 February 2024 (09:34:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.2017.v1

Antiviral Activity of Anthranilamide Peptidomimetics against Herpes Simplex Virus 1 and a Coronavirus

Umme Laila Urmi, Samuel Attard, Ajay Kumar Vijay, Mark D. P. Willcox, Naresh Kumar, Salequl Islam, Rajesh Kuppusamy

Subject: Biology And Life Sciences, Virology Keywords: antiviral compounds; peptidomimetics; MHV-1; HSV-1; envelope disruption

Online: 30 August 2023 (07:21:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0090.v1

IL-1R8 Downregulation and Concomitant TLR7 and TLR9 Up-Regulation Are Related to the Pathogenesis of Canine Diffuse Large B-Cell Lymphoma

Federica Riva, Joel Filipe, Antonella Fanelli, Laura Marconato, Alessia Inglesi, Eugenio Scanziani, Sabina Soldati, Luca Licenziato, Stefano Comazzi, Lucia Minoli, Luca Aresu

Subject: Medicine And Pharmacology, Veterinary Medicine Keywords: dog; diffuse large B-cell lymphoma; IL-1R8; TLR7; TLR9; qRT-PCR

Online: 11 April 2022 (09:15:27 CEST)

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Preprint REVIEW | doi:10.20944/preprints202008.0020.v1

SARS-CoV-2 and Covid-19 Immunopathogenesis

Antonio Luiz Boechat, Beatriz Pessoa, Carlos Soares, Cecília Barroso, David Vila, Emanuelly Barbosa, Isabela Seffair, João Victor Melo, Julia Becil, Maria Polyanna Rebouças, Natascha Rodrigues, Pedro Henrique Freitas, Rebeka Rocha, Thaise Rodrigues, Vanessa Ferreira, Rosmery Ubiera, Maria Cristina Dos-Santos

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: SARS-CoV-2; COVID-19; Immunopathogenesis; Cytokine storm; IL-6; macrophages; neutrophils

Online: 2 August 2020 (11:53:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202006.0093.v1

Thymic Stromal Lymphopoietin promoted CD4⁺CD25^- T cells differentiation isolated from the thymus of patients with Myasthenia Gravis to CD4⁺CD25⁺ Regulatory T Cells In Vitro

Jiang Yuan, Na Wang, Md Rezaul Karim, Lei Zeng, Qiang Sun, Yanpeng Sun, Hong-Yan Zhang, Yun-Fu Wang

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: myasthenia gravis; thymic stromal lymphopoietin; regulatory T cells; TGF- β; IL-10

Online: 7 June 2020 (15:00:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints201905.0040.v1

Molecular Mechanisms of Cancer-Induced Sleep Disruption

William H. Walker II, Jeremy C. Borniger

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: breast cancer; sleep; IL-6; hypocretin/orexin; leptin; EEG; autonomic nervous system

Online: 6 May 2019 (08:52:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201810.0467.v1

Towards Biohydrogen Separation Using Poly(ionic liquid)/ionic Liquid Composite Membranes

Andreia S.L. Gouveia, Lucas Ventaja, Liliana C. Tome, Isabel M. Marrucho

Subject: Engineering, Bioengineering Keywords: drogen purification; CO2/H2 separation; PIL–IL composite membranes; gas permeation properties

Online: 22 October 2018 (05:18:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0001.v1

UTI Biomarkers in Symptomatic Patients are Significantly Elevated at Microbial Densities of 10,000 cells or CFUs per mL Indicating a Lower Microbial Density Threshold for Diagnosing UTIs

Laura Parnell, Natalie Luke, Mohit Mathur, Richard Festa, Emery Haley, Jimin Wang, Yan Jiang, Lori Anderson, David Baunoch

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: urinary tract infection (UTI); standard urine culture (SUC); multiplex polymerase chain reaction (M-PCR); urine biomarkers; diagnostic testing; neutrophil gelatinase-associated lipocalin (NGAL); interleukin (IL)-8, IL-1β

Online: 3 July 2023 (09:28:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.1004.v1

Depletion of Endothelial-Derived 2-AG Reduces Blood-Endothelial Barrier Integrity via Alteration of VE-Cadherin and the Phospho-Proteome

Aidan A Levine, Erika Liktor-Busa, Shreya Balasubramanian, Seph M Palomino, Anya M Burtman, Sarah A Couture, Austin A Lipinski, Paul R Langlais, Tally M Largent-Milnes

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: endocannabinoid; blood-brain barrier; 2-AG, DAGLα ; VE-cadherin

Online: 14 December 2023 (04:52:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1168.v1

Exploring Polygonal Number Sieves through Computational Triangulation

Sergei Abramovich

Subject: Computer Science And Mathematics, Mathematics Keywords: computation; number theory; triangulation; polygonal numbers; spreadsheet; Wolfram Alpha; Maple

Online: 20 November 2023 (07:51:53 CET)

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Preprint REVIEW | doi:10.20944/preprints202308.1555.v1

Overview of the Biotransformation of Limonene and α-Pinene from Wood and Citrus Residues by Microorganisms

Adama Ndao, Kokou Adjallé

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: limonene; alpha-pinene; biotransformation; terpene; flavouring agent; solid-state fermentation

Online: 23 August 2023 (02:49:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202105.0743.v1

Applications of PERALS-Alpha Spectrometry for the Investigation of Radionuclides in Water Samples

Markus Raymond Zehringer, Franziska Kammerer, Anja Pregler

Subject: Physical Sciences, Acoustics Keywords: PERALS; alpha spectrometry; Polonium; Radon; Radium; Radiostrontium; Thorium; Uranium; Actinides

Online: 31 May 2021 (11:16:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201909.0177.v1

Forest Diversity and Structure in the Amazonian Mountains Ranges of Southeastern Ecuador

Wilson Quizhpe, Angel Benítez, Jesus Muñoz, Klever Cuenca, Hernán Uvidia, Isau Huamantupa, Omar Cabrera

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: alpha diversity; canonical correspondence; NMDS; Sandstones Forests; Terra firme Forests

Online: 17 September 2019 (03:48:02 CEST)

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1) Background: We analyzed the size structure, richness and diversity in eight permanent forest plots in the upper limit of the great Amazon basin located in the Ecuadorian parts of Cordilleras del Cóndor and Cutucú. Our hypothesis was that not all the forests of the eastern mountain ranges are homogeneous in characteristics, but rather present differences in their structure, richness and floristic diversity. In order to test this, our main objective was to classify the types of forests based on characteristics in diametric structure and species composition of the Amazonian forests of the eastern cordilleras in southern Ecuador, and also to determine the influence of critical edaphic-environmental and geomorphological factors. 2) Methods: Eight one-hectare permanent plots were installed in homogeneous and well-conserved forest stands. Four plots were located in the province of Zamora Chinchipe and four in the province of Morona Santiago. We identified and measured all trees> 10 cm at diameter breast height. For each plot, soil samples were taken, along with environmental and slope data. The multidimensional non-metric adjusted scaling (NMDS) was used to evaluate changes in climatic and geomorphological gradients. The relationship between the composition of the species at the plot level and the edaphoclimatic variables was tested using a canonical correspondence analysis (CCA). Finally, we modeled the change in species diversity (Fisher's alpha) in relation to the climatic gradients, altitudinal and geomorphological using a GLMM. 3) Results: Overall, we identified 517 species belonging to 76 families. We determined the existence of two different types of forest, the first one named Terra Firme, characterized by the presence of a larger number of species and individuals per plot, compared to the second type of forest, called Tepuy or Sandstones forest. Species richness was negatively correlated with soil phosphorus content and pH, mean annual temperature, annual precipitation and altitude. The CCA analysis showed differences in the species composition between the Terra Firme and Sandstone forests. The climatic seasonality and the concentration of cations in the soil influence the diversity of the Amazon forest communities of the southeastern cordilleras. The forests of Terra Firme, which are settled in more stable and nutrient-rich climatic areas, were more diverse. Sandstone forests are poor in nutrients and develop in areas with stronger seasonality

Preprint ARTICLE | doi:10.20944/preprints201804.0085.v3

Vertex Domination in Fuzzy Graphs

Mohammadesmail Nikfar

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: fuzzy graph; fuzzy bridge; fuzzy tree; $\alpha$-strong arc; vertex domination

Online: 5 May 2019 (12:20:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201810.0391.v1

Wheat ATI CM3, CM16 and 0.28 Allergens Produced in Pichia Pastoris Display a Different Eliciting Potential in Food Allergy to Wheat

Silvio Tundo, Roberta Lupi, Mickael Lafond, Thierry Giardina, Colette Larré, Sandra Denery-Papini, Martine Morisset, Raviraj Kalunke, Francesco Sestili, Stefania Masci

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: wheat; alpha-amylase/trypsin inhibitor; heterologous expression; Pichia pastoris; allergy

Online: 17 October 2018 (17:15:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.0002.v1

Transcriptomic Analysis of CRISPR/Cas9-Mediated TDP1-Knockout HEK293A Cells

Nadezhda S. Dyrkheeva, Alexandra L. Zakharenko, Anastasia A. Malakhova, Larisa S. Okorokova, Dmitry N. Shtokalo, Sergey P. Medvedev, Alexey A. Tupikin, Marsel R. Kabilov, Olga I. Lavrik

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: tyrosyl-DNA phosphodiesterase 1; topoisomerase 1; HEK293A; transcriptome; TDP1 knockout

Online: 1 December 2023 (04:53:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1479.v1

Interplay of Serum Leptin, Tumor Necrosis Factor Alpha, and Testosterone in Overweight-Related Hypertensive Men

Shalan Alaamri, Abdulhalim S Serafi, Zahir Hussain, Shouq K. Bafail, Mohammed A. Bafail, Lusine H. Demirkhanyan, Christopher S Gondi, Sumera Sohail

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Hypertension; overweight-related hypertension; serum leptin; TNF-α, testosterone; total cholesterol; IL-6

Online: 21 June 2023 (08:43:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0987.v1

Iguratimod Ameliorates the Severity of Secondary Progressive Multiple Sclerosis in Model Mice by Directly Inhibiting IL-6 Production and Th17 Cell Migration via Mitigation of Glial Inflammation

Satoshi Nagata, Ryo Yamasaki, Ezgi Ozdemir Takase, Kotaro Iida, Mitsuru Watanabe, Katsuhisa Masaki, Marion Heleen Cathérine Wijering, Hiroo Yamaguchi, Jun-ichi Kira, Noriko Isobe

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: iguratimod; secondary progressive multiple sclerosis; connexin 47; experimental autoimmune encephalomyelitis; IL-6; astrocyte

Online: 14 June 2023 (04:59:11 CEST)

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Working Paper ARTICLE

WNT/β-catenin Signaling Promotes TGF-β-mediated Activation of Human Cardiac Fibroblasts by Enhancing IL-11 Production

Edyta Działo, Marcin Czepiel, Karolina Tkacz, Maciej Siedlar, Gabriela Kania, Przemysław Błyszczuk

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: cardiac fibroblasts; WNT3a; WNT5a; beta-catenin; TGF-beta signalling; IL-11; cardiac fibrosis

Online: 29 July 2021 (13:15:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201810.0686.v1

Macrophages from Mice Administered Rhus Verniciflua Stokes Extract Show Selective Anti-Inflammatory Activity

Bo-Geun Kim, Youngju Song, Mi-Gi Lee, Jin-Mo Gu, SeungGwan Lee, Hee Kang

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Rhus verniciflua Stokes; in vivo; inflammation; macrophage; monocyte differentiation; MHC II; IL-12

Online: 29 October 2018 (13:26:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints201809.0424.v1

Genistein Loaded Nanofibers Protect Spinal Cord Tissue Following Experimental Injury in Rats

Mohamed Ismail, Sara Ibrahim, Azza Elamir, Amira M. Elrafei, Nageh Allam, Ahmed Abdellatif

Subject: Medicine And Pharmacology, Pharmacy Keywords: Genistein; nanofibers; spinal cord injury; inflammation; SOD; NO; MDA; IL-10; TNF-α

Online: 20 September 2018 (17:11:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0398.v1

An Immune Escape of Oral Squamous Cell Carcinoma Might Be Different in the Originating-Epithelium, Keratinized or Non-keratinized

Yoshiaki Kistukawa, Chonji Fukumoto, Toshiki Hyodo, Yuske Komiyama, Ryo Shiraishi, Aya Koike, Shuma Yagisawa, Yosuke Kunitomi, Tomonori Hasegawa, Wataru Kotani, Kazuyuki Ishida, Takahiro Wakui, Hitoshi Kawamata

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: immune escape; tumor immunity; immune checkpoint; oral squamous cell carcinoma; programmed death receptor-1 (PD-1); programmed cell death 1-ligand 1 (PD-L1); CD47

Online: 7 March 2024 (07:49:57 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0202.v1

Accumulation of Alpha-Synuclein and Increase in the Inflammatory Response in the Substantia Nigra, Jejunum, and Colon, in a Model of O3 Pollution in Rats

Marlen Valdés-Fuentes, Erika Rodríguez-Martínez, Selva Rivas-Arancibia

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Ozone pollution; neurodegeneration; Alpha-synuclein; inflammation; NFκB; Th17; substantia nigra; gut

Online: 2 April 2024 (12:16:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1574.v1

Evaluation of Safety and Efficacy of Repeated Mesenchymal Stem Cell Transplantations in ALS Patients by Investigating Patient's Specific Immunological and Biochemical Biomarkers: A Retrospective study.

Zahraa Alkhazaali, Sajad Sahab-Negah, Amir Reza Boroumand, Najmeh Kaffash, Mohammad Ali Khodadoust, Jalil Tavakol Afshari

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: ALS; Tumor Necrosis Factor-alpha; GDNF; Creatine kinase; ferritin; BM-MSC.

Online: 26 March 2024 (09:15:36 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1605.v1

Autoprobiotics in the treatment of patients with colorectal cancer in the early postoperative period

Elena I Ermolenko, Natalia V Baryshnikova, Galina Alekhina, Alexander Zakharenko, Oleg Ten, Victor A Kashchenko, Nadezhda Lavrenova, Olga Gushchina, Timofey Ovchinnikov, Anastasia Morozova, Anastasia Ilina, Alena B. Karaseva, Anna Tsapieva, Nikita Gladyshev, Alexander V. Dmitriev, Alexander Suvorov

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: microbiome, autoprobiotics, Enterococcus spp., Parvomonas micra, alpha diversity, gut dysbiosis, interleukins

Online: 28 February 2024 (08:05:17 CET)

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Introduction: Despite great advances in the treatment of oncological diseases, the development of medical technologies to prevent or reduce complications of therapy, in particular, those associated with surgery and the introduction of antibiotics remains relevant. The aim of the study: to evaluate the effectiveness of the use of autoprobiotics based on indigenous non-pathogenic strains of Enterococcus faecium and Enterococcus hirae in the complex therapy of colorectal cancer (CRC) in the early postoperative period. Materials and methods: A total of 36 patients diagnosed with CRC were enrolled in the study. The study group (Group A) comprised 24 CRC patients who received autoprobiotic therapy in the early postoperative period, while the control group (Group C) included 12 CRC patients without autoprobiotic therapy.Prior to surgery and between days 14-16 post-surgery, comprehensive evaluations were conducted on all patients, encompassing the following: stool and gastroenterological complains analysis. examination of the gut microbiota (bacteriological study, quantitative polymerase chain reaction, metagenome analysis), analysis of interleukins in serum. Results: The use of autoprobiotics led to decrease dyspeptic complaints after surgery, was associated with absence of postoperative complications, did not cause side effects, led to a decrease in the level of pro-inflammatory cytokines (IL-6 and IL-18) in the blood serum. The use of autoprobiotics led to positive changes in the structure of escherichia and enterococci populations, the elimination of Parvomonas micra, Fusobacterium nucleatum, decrease in the quantitative content of Clostridium perfringens, Akkermansia muciniphila. Metagenomic analysis (16S rRNA) revealed an increase in alpha diversity. Conclusion: The administration of autoprobiotics in the postoperative period is highly effective and safe in the comprehensive treatment of colorectal cancer patients. Future studies will allow the discovery of additional fine mechanisms of autoprobiotic therapy and its impact on the digestive, immune, endocrine and neural systems.

Preprint ARTICLE | doi:10.20944/preprints202402.1050.v1

Seasonal Variation in the Gut Microbiota of Athletes: A Comparative Study of Japanese Handball Players

Kazuya Toda, Shin Yoshimoto, Keisuke Yoshida, Eri Mitsuyama, Noriyuki Iwabuchi, Koji Hosomi, Takayuki Jujo Sanada, Miyuki Tanaka, Hinako Nanri, Jun Kunisawa, Toshitaka Odamaki, Motohiko Miyachi

Subject: Biology And Life Sciences, Life Sciences Keywords: Gut microbiota; athletes; longitudinal study; alpha-diversity; Japanese male handball players

Online: 20 February 2024 (04:36:11 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202402.0868.v1

Alpha7 Nicotinic Acetylcholine Receptor Agonist PHA 568487 Does Not Affect Cardiac Function nor Myocardial Infarct Size in the Permanent Occlusion Model

Filip Mjörnstedt, Azra Miljanovic, Malin Levin, Maria E Johansson

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: alpha 7 nicotinic acetylcholine receptor; α7nAChR; myocardial infarction; inflammation; α7nAChR agonist

Online: 16 February 2024 (11:34:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1511.v1

Transpiration Dynamics of Esparto Grass (Stipa tenacissima L.) in Semi-Arid Mediterranean Climate: Unraveling the Impacts of Soil Water, Pine Competence, and Bush Maturity

Iván Pérez-Anta, Eva Rubio, Francisco Ramón López-Serrano, Diego Garcés, Manuela Andrés, Marta Picazo, Wafa Chebbi, Rocío Arquero, Francisco Antonio García-Morote

Subject: Biology And Life Sciences, Forestry Keywords: Alpha-grass; Water exchange; Pine competence; Semi-arid woodlands; Leaf senescence

Online: 19 January 2024 (14:04:11 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.0235.v1

Cellular Strategies for Separating GvHD from GvL in Haploidentical Transplantation

Mauro Di Ianni, Carmine Liberatore, Nicole Santoro, Paola Ranalli, Francesco Guardalupi, Giulia Corradi, Ida Villanova, Barbara Di Francesco, Stefano Lattanzio, Cecilia Passeri, Paola Lanuti, Patrizia Accorsi

Subject: Public Health And Healthcare, Primary Health Care Keywords: GvHD; GvL; TCR alpha-beta; CD45RA; Tregs; NK; Suicide gene; immunotherapies

Online: 5 December 2023 (09:18:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.0650.v1

Association between Salivary Cortisol and α-amylase with the Psychological Profile of Patients with Oral Lichen Planus and Burning Mouth Syndrome: A Cross-Sectional Study

Ana Glavina, Liborija Lugović-Mihić, Dinko Martinović, Livia Cigić, Ledia Tandara, Marino Lukenda, Dolores Biočina-Lukenda, Daniela Šupe-Domić

Subject: Medicine And Pharmacology, Dentistry And Oral Surgery Keywords: Alpha-Amylase; Biomarkers; Burning Mouth Syndrome; Cortisol; Oral Lichen Planus; Saliva.

Online: 11 July 2023 (09:24:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202001.0038.v1

Responses of Individual Plant Species, Functional Groups, α- and β-Diversity to Short-Term Grazing Exclusion under Severe Drought Episode in Long-Term Grazed Alfa-Steppes

Yacine Kouba, Saifi Merdes, Badreddine Saadali, Haroun Chenchouni

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Mediterranean steppes; drylands; alpha diversity; beta diversity; rangeland grazing; indicator species

Online: 5 January 2020 (15:22:33 CET)

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Grazing exclusion has been proved to be one of the main measures for rehabilitating degraded arid steppes. However, the effect of this management practice on plant species diversity and composition is ambiguous, specially under prolonged droughts. Concurrently considering the responses of individual plant species, diversity of functional groups, α-diversity, and β-diversity (and its components) may be crucial to the holistic understanding of grazing exclusion effects on plant communities under drought conditions. Here, we investigated the response of these diversity measures to short-term sheep exclusion under severe drought episode in arid steppes of Alfa-grass (Stipa tenacissima) with a long evolutionary history of livestock grazing. Individual species responses were tested based on species occurrence and abundance in either grazed or grazing-excluded steppes, in addition, we used indicator species analysis to assess the strength of the association between plant species and management type. Likewise, α-diversity, abundance- and incidence-based β-diversity, as well as the functional groups’ diversities were quantified using Hill Numbers and compared between the two management types. Sheep grazing exclusion enabled the recovery of various Alfa-steppe indicator species and improved the size of regional species pool, overall α-diversity, and the diversity of therophytes. This management practice decreased the abundance-based β-diversity and the nestedness-resultant fraction of the incidence-based β-diversity at the local scale, while at the landscape scale increased the abundance-based β-diversity and its balanced variation fraction and reduced the incidence-based β-diversity and its turnover component. Furthermore, protection from grazing altered β-diversities scaling patterns by maintaining higher balanced variation in species abundance at large spatial scale and greater abundance-gradient in species composition at the fine-scale. Our results suggest that the implementation of short-term grazing exclusion in degraded arid steppes would be the appropriate management practice for vegetation restoration and plant diversity conservation during prolonged drought periods.

Preprint CONCEPT PAPER | doi:10.20944/preprints201809.0209.v1

Paths for Improving Bevacizumab Available in 2018: The ADZT Regimen for Better Glioblastoma Treatment

Richard Kast

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: ADZT regimen; apremilast; bevacizumab; cAMP; dapsone; glioblastoma; telmisartan; TNF-alpha; zonisamide

Online: 12 September 2018 (03:54:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201609.0073.v1

Strategies in the Design of Antidiabetic Drugs from Terminalia Chebula Retz. Using in silico and in vitro Approach

Twinkle Bansode, B.K. Salalkar

Subject: Medicine And Pharmacology, Endocrinology And Metabolism Keywords: diabetes mellitus, molecular docking, thin layer chromatography, alpha amylase, ellagic acid

Online: 20 September 2016 (16:13:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0363.v1

The Virus-induced Upregulation of the miR-183/96/182 Cluster and the FoxO Family Protein Members are not Required for the Efficient Replication of HSV-1

Andreja Zubković, Ines Žarak, Ivana Ratkaj, Filip Rokić, Maja Badurina, Marina Pribanić Matešić, Ricardo Lebron, Cristina Gómez-Martín, Berislav Lisnić, Vanda Juranic Lisnic, Stipan Jonjić, Dongli Pan, Oliver Vugrek, MIchael Hackenberg, Igor Jurak

Subject: Biology And Life Sciences, Virology Keywords: herpes simplex virus 1; HSV-1; virus-host interaction; miRNA; FoxO

Online: 26 May 2022 (10:34:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202106.0565.v1

Targeting Tat-TAR RNA Interaction for HIV-1 Inhibition

Awadh Alanazi, Andrey Ivanov, Namita Kumari, Xionghao Lin, Songping Wang, Dmytro Kovalskyy, Sergei Nekhai

Subject: Biology And Life Sciences, Virology Keywords: HIV-1 transcription; HIV-1 Tat; TAR RNA; small molecule inhibitors

Online: 23 June 2021 (11:04:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0138.v1

Comparison of Forecasting Ability for Energy Consumption in BRICS: ARIMA (1,1,1) and FGM (1, 1) Models

Atif Maqbool Khan, Magdalena Osinska

Subject: Business, Economics And Management, Accounting And Taxation Keywords: Energy consumption; BRICS; GM (1, 1); Fractional-order; GREY; Forecasting accuracy

Online: 5 April 2021 (13:51:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202012.0796.v1

The Role of Tissue Resident Memory CD4 T Cells in Herpes Simplex Viral and HIV Infection

Thomas Ray O'Neil, Kevin Hu, Naomi Truong, Sana Arshad, Barbara Shacklett, Anthony Laurence Cunningham, Najla Nasr

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: HIV-1; HSV-1/2; CD4; CD8; Vaccines; Infection; Immunity; Keratitis

Online: 31 December 2020 (12:18:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202107.0484.v1

Metformin Sensitizes Leukemic Cells to Cytotoxic Lymphocytes by Increasing Expression of Intercellular Adhesion Molecule-1 (ICAM-1)

Nerea Allende-Vega, Joaquin Marco Brualla, Paolo Falvo, Catherine Alexia, Michael Constantinides, Alexis Fayd-Herbe-de-Maudave, lois coenon, delphine gitenay, Giulia Mitola, paul massa, stefania orecchioni, Francesco Bertolini, Isabel Marzo, Alberto Anel, Martin Villalba

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: metformin; Natural Killer cells; Cytotoxic T lymphocytes; intercellular adhesion molecule-1 (ICAM-1); Natural Killer G2-D (NKG2D) ligands (NKG2DL); lymphocyte function-associated antigen 1 (LFA-1)

Online: 21 July 2021 (10:54:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1058.v1

Mesothelioma Associated Fibroblasts Modulate the Response of Mesothelioma Patient Derived Organoids to Chemotherapy through Interleukin-6.

Mario Cioce, Veronica Gatti, Fabiana Napolitano, Noemi Maria Giorgiano, Andrea Marra, Giuseppe Portella, Alfonso Fiorelli, Francesca Pentimalli, Vito Michele Fazio

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Mesothelioma patient-derived organoids, PDO, IL-6, mesothelioma-associated fibroblasts; cocultures, cisplatin; pemetrexed; chemoresistance

Online: 18 March 2024 (15:17:01 CET)

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Background. Malignant pleural mesothelioma (MPM) is still a rather incurable disease. Many classes of compounds with various mechanisms of action have been tested in vivo and ex-vivo, with rather disappointing results. This is partly due to preclinical studies mostly performed in models that fail to recapitulate MPM complexity and do not retain the intra-tumor heterogeneity, a propelling force for tumor progression. Additionally, we and others have shown that, under stress-induced chemotherapy, profound rearrangements take place in MPM cultures to fuel the emergence of chemoresistant cell subpopulations. Thus, finding clinically actionable modulators of MPM resistance is still a very urgent need. Methods. Setup and propagation of patient-derived organoid cultures from MPM pleural effusions; flow cytometry-based validation of organoid cultures, isola-tion and propagation of mesothelioma-associated fibroblasts; co-cultures, Lu-minex-based Cytokine array, ELISA assay to quantitate IL-6 in the conditioned media, qRT-PCR to assess mRNA expression of cancer stem cell markers and fibroblast markers. Results. Organoid cultures were obtained from eight MPM patients by using specific growth media and conditions to propagate pleural effusion-derived cells. We proved that primary MPM specimens and serially passaged (mesothelio-ma-Patient-Derived-Organoids) mPDOs were cytologically similar, with the obtained mPDOs expressing mesothelial markers similarly to the originating specimen. As expected from a clinically relevant model, the size and number of mPDOs were altered, in a patient-specific way, when pharmacologically relevant doses of chemotherapy drugs were added. We derived Mesothelio-ma-Associated-Fibroblasts (MAFs) and the addition of MAFs, to various ratios, altered the response of the mPDOs to pemetrexed + cisplatin, toward a reduced sensitivity to the latter anticancer agents. Additionally, organoid formation and size were altered by MAF-conditioned media, and this correlated with increased expression of cancer stem cell markers. We showed increased levels of IL-6 in the media of the treated cocultures and showed that this latter was derived from the MAFs. Conclusions. Increased secretion of IL-6 by mesothelioma-associated fibroblasts (MAFs) contributes to the resistance of the MPM to therapy.

Preprint REVIEW | doi:10.20944/preprints202402.1548.v1

Exploring the Landscape of Anti-Inflammatory Trials: A Comprehensive Review of Strategies for Targeting Inflammation in Acute Myocardial Infraction

Andreas Mitsis, Michaela Kyriakou, Stefanos Sokratous, Georgia Karmioti, Michail Drakomathioulakis, Michael Myrianthefs, Antonios Ziakas, Stergios Tzikas, George Kassimis

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Acute myocardial infarction; anti-inflammatory interventions; canakinumab; colchicine; coronary artery disease; IL-6; inflammation

Online: 27 February 2024 (11:52:23 CET)

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Preprint HYPOTHESIS | doi:10.20944/preprints202004.0143.v1

Inflamm-Aging: Why Older Men Are the Most Susceptible to SARS-Cov-2 Complicated Outcomes

Massimiliano Bonafè, Francesco Prattichizzo, Angelica Giuliani, Gianluca Storci, Jacopo Sabbatinelli, Fabiola Olivieri

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: SARS-CoV-2; COVID-19; IL-6; inflamm-aging; immune senescence; host-directed therapies

Online: 9 April 2020 (08:38:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.1501.v1

Selected Plant Triterpenoids and Their Derivatives as Antiviral Agents

Martina Wimmerová, Uladzimir Bildziukevich, Zdenek Wimmer

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: plant triterpenoid; structure modifier; antiviral activity; HIV-1; HSV-1; maturation inhibitor

Online: 24 October 2023 (07:33:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202212.0547.v1

First Screening of Beta-Lactam Antibiotic Resistance Genes and Bacterial Diversity in the Public Transport System of Quito, Ecuador

Fernanda Gabriela Hernandez-Alomia, Carlos Andrès Bastidas-Caldes, Isabel Ballesteros, Gabriela N. Tenea, Pablo Santiago Jarrin, Carlos Alfonso Molina, Pablo Castillejo

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: mecA; blaTEM-1; blaOXA-181; blaCTX-M-1; environmental-DNA; antibiotic-resistance

Online: 29 December 2022 (02:05:52 CET)

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Preprint ARTICLE | doi:10.20944/preprints201702.0054.v1

Identification of the Sixth Ig-like Domain of VCAM-1 as a Novel Therapeutic Target in Lung Cancer Cell Invasion

Mi Ra Kim, Ji Hye Jang, Chang Sik Park, Taek-Keun Kim, Youn-Jae Kim, Junho Chung, Hyunbo Shim, In Hyun Nam, Jung Min Han, Sukmook Lee

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: human antibody; invasion; lung cancer; therapeutic target; VCAM-1; VCAM-1-D6

Online: 15 February 2017 (10:45:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.0328.v1

Lonicera japonica Thunb. Ethanol Extract Exerts a Protective Effect on Normal Human Gastric Epithelial Cells by Modulating the Activity of Tumor-Necrosis-Factor-α-Induced Inflammatory Cyclooxygenase 2/Prostaglandin E2 and Matrix Metalloproteinase 9

Hsi-Lung Hsieh, Ming-Chin Yu, Yu-Chia Chang, Yi-Hsuan Wu, Kuo-Hsiung Huang, Ming-Ming Tsai

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Lonicera japonica Thunb.; Matrix metallopeptidase-9; Tumor necrosis factor-α ; Cyclooxygenase-2

Online: 4 April 2024 (12:58:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1417.v1

Phenotypic Differences among Recently Diagnosed Drug-Naïve Patients with Parkinson’s Disease with or without SNCA Polymorphisms

Santiago Pérez-Lloret, Alejandro Enet, Guenson Chevalier, Sofia Bordet, Hanny Barbar, Francisco Capani, Lucas Udovin, Matilde Otero-Losada, Francisco Barrantes

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: single nucleotide polymorphism; Parkinson’s disease; alpha-synuclein; phenotype, pathophysiology; PD-related variants

Online: 20 July 2023 (12:46:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0308.v1

Effects of Different Feeding Patterns on the Rumen Bacterial Community of Tan Lambs, based on High-Throughput Sequencing of 16S rDNA Amplicons

Lili Zhang, Yanliang Bi, Jie Zhang, Zilin Fu, Xiaofeng Xu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Alpha diversity; Beta diversity; Mutton quality; Pasture-fed; Pen-fed; Tan lambs

Online: 13 April 2023 (08:01:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202212.0276.v1

Rethinking the Paradoxical Increase in Endogenous Glucose Production in Response to Metformin: Should We Retain the Glucagon Antagonistic Effect of the Drug?

Jabeur Methnani, Taieb Ach, Aymen El Hraiech, Imed Latiri, Ali Bouslama, Monia Zaouali, Asma omezzine, Ezdine Bouhlel

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Biguanides; Antihyperglycemic agents; Liver alpha-cell axis; gluconeogenesis; Protein metabolism; insulin sensitivity

Online: 15 December 2022 (08:37:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202205.0184.v1

Amyloid Precursor Protein, Alpha-, Beta- and Gamma-Secretases Expression in Penumbra Cells after Photothrombotic Stroke. Evaluation of the Neuroprotective Effect of Secretase Inhibitors

Svetlana Sharifulina, Valeria Guzenko, Alexandr Logvinov, Andrey Khaitin, Yuliya Kalyuzhnaya, Natalia Dobaeva, Yan Li, Lei Chen, Вin He, Svetlana Demyanenko

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloid precursor protein; photothrombotic stroke; ischemia; alpha-secretase; beta-secretase; gamma-secretase

Online: 13 May 2022 (08:39:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0526.v1

Effect of α-Hemolysin Producing E. coli in Two Different Mouse Strains in a DSS Model of Inflammatory Bowel Disease

Hengameh Chloé Mirsepasi-Lauridsen, Carsten Struve, Andreas Munk Petersen, Karen Angeliki Krogfelt

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: inflammatory-bowel-disease; Sigirr -/- mice; C57BL/6-mice; Escherichia coli; alpha hemolysin

Online: 20 November 2020 (09:14:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints201906.0052.v1

SerThr-PhosphoProteome of Brain from Aged PINK1-KO+A53T-SNCA Mice Reveals pT1928-MAP1B and pS3781-ANK2 Deficits, as Hub between Autophagy and Synapse Changes

Georg Auburger, Suzana Gispert, Sylvia Torres-Odio, Marina Jendrach, Nadine Brehm, Júlia Canet-Pons, Jana Key, Nesli-Ece Sen

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Parkinson's disease; brain phosphorylome; PINK1, alpha-synuclein; microtubular cytoskeleton; autophagy; synaptic signaling

Online: 7 June 2019 (03:21:19 CEST)

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Hereditary Parkinson’s disease (PD) can be triggered by an autosomal dominant overdose of alpha-Synuclein (SNCA) as stressor or the autosomal recessive deficiency of PINK1 Serine/Threonine-phosphorylation activity as stress-response. We demonstrated the combination of PINK1-knockout with overexpression of SNCAA53T in double mutant (DM) mice to exacerbate locomotor deficits and to reduce lifespan. To survey posttranslational modifications of proteins underlying the pathology, brain hemispheres of old DM mice underwent quantitative label-free global proteomic mass spectrometry, focused on Ser/Thr-phosphorylations. As exceptionally strong effect, we detected >300-fold reductions of phosphoThr1928 in MAP1B, a microtubule-associated protein, and a similar reduction of phosphoSer3781 in ANK2, an interactor of microtubules. MAP1B depletion is known to trigger perturbations of microtubular mitochondria trafficking, neurite extension and synaptic function, so it was noteworthy that relevantly decreased phosphorylation was detected also for other microtubule and microfilament factors, namely MAP2S1801, MARK1S394, MAP1AT1794, KIF1AS1537, 4.1NS541, 4.1GS86 and ADD2S528. While the MAP1B heavy chain supports regeneration and growth cones, its light-chain assists DAPK1-mediated autophagy. Interestingly, relevant phosphorylation decreases of DAPK2S299, VPS13DS2429 and VPS13CS2480 in the DM brain affected regulators of autophagy, which are implicated in PD. Overall, significant downregulations were enriched for PFAM C2 domains, other kinases, and synaptic transmission factors upon automated bioinformatics, while upregulations were not enriched for selective motifs or pathways. Validation experiments confirmed the change of LC3 processing as reflection of excessive autophagy in DM brain, and dependence of ANK2/MAP1B expression on PINK1 levels. Our new data provide independent confirmation in a mouse model with combined PARK1/PARK4/PARK6 pathology that MAP1B/ANK2 phosphorylation events are implicated in Parkinsonian neurodegeneration. These findings expand on previous observations in D. melanogaster that the MAP1B ortholog futsch in the presynapse is a primary target of the PARK8 protein LRRK2, and on a report that MAP1B is a component of the pathological Lewy body aggregates in PD patient brains. Similarly, ANK2 gene locus variants are associated with the risk of PD, ANK2 interacts with PINK1/Parkin-target proteins such as MIRO1 or ATP1A2, and ANK2-derived peptides are potent inhibitors of autophagy.

Preprint ARTICLE | doi:10.20944/preprints201810.0283.v1

Geomorphology and Altitude Effects on Diversity and Structure of Vanishing Montane Forest of Southern Ecuador

Omar Cabrera, Angel Benítez, Nixon Cumbicus, Carlos Naranjo, Pablo Ramón, Fani Tinitana, Adrián Escudero

Subject: Biology And Life Sciences, Forestry Keywords: alpha diversity; beta diversity; floristic arrangement; geological landscapes; geomorphology; Ecuador; mountain forests

Online: 15 October 2018 (06:19:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0365.v1

Enantioselective Organocatalyzed a-Amination of 1,3-Dicarbonyl Compounds in Deep Eutectic Mixtures

Diego Ros Ñíguez, Pegah Khazaeli, Diego A. Alonso, Gabriela Guillena

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: asymmetric organocatalysis; benzimidazole; alpha-Amination; deep eutectic solvents; green chemistry, natural products

Online: 27 April 2018 (16:23:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201709.0100.v1

Cut-off Values of Hematologic Parameters to Predict the Number of Alpha Genes Deleted in Subjects with Deletional Alpha Thalassemia

Diego Velasco-Rodríguez, Carlos Blas, Juan-Manuel Alonso-Domínguez, Gala Vega, Carlos Soto, Aránzazu García-Raso, Pilar Llamas-Sillero

Subject: Medicine And Pharmacology, Hematology Keywords: alpha; thalassemia; deletional; cut-off; number of genes; microcytic anemia; differential diagnosis

Online: 21 September 2017 (04:47:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202301.0541.v9

A Solution of The Collatz Conjecture Problem

baoyuan duan

Subject: Computer Science And Mathematics, Signal Processing Keywords: Collatz conjecture; (*3+1)/2^k odd sequence; (*3+2^m-1)/2^k odd sequence; (*3+2^m-1)/2^k odd tree; weight function

Online: 21 July 2023 (08:53:32 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202005.0144.v1

Pyridoxal 5'-phosphate to mitigate immune dysregulation and coagulopathy in COVID-19

Julie Desbarats

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: COVID-19, SARS-CoV-2, pyridoxal 5'-phosphate, pyridoxine, vitamin B6, immune response, IL-6, TNF, type I interferon, lymphopenia, blood clotting, coagulopathy, cytokine storm, sphingosine-1-phosphate, kynurenine, inflammasome, serine hydroxymethyltransferase 2 (SHMT2), hypertension, angiotensin

Online: 8 May 2020 (12:36:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0397.v1

Erythrocytes of Nonalcoholic Steatohepatitis Patients Contain Decreased Arginase-1 and Thrombospondin-1, and Increased Phosphatidylethanolamine Levels

Charalampos Papadopoulos, Konstantinos Mimidis, Ioannis Tentes, Konstantinos Anagnostopoulos

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: erythrocyte; non alcoholic steatohepatitis; immunometabolism; thrombospondin-1; arginase-1; phosphatidylethanolamine; metabolic inflammation ;

Online: 17 April 2023 (04:22:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0120.v1

Mathematical Modeling of the Growth Suppression of HER2+ Breast Cancer Colonies Under the Effect of Trastuzumab and PD-1/PD-L1 Inhibitor

Regina Padmanabhan, Hadeel Kheraldine, Ishita Gupta, Nader Meskin, Anas Hamad, Semir Vranic, Ala-Eddin Al Moustafa

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: HER2; PD-1/PD-L1; Mathematical model; HER2/PD-1 Interaction; Breast cancer

Online: 9 May 2022 (14:06:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1115.v1

Low Number of Baselines γδ T Cells Increase the Risk of SARS-CoV-2 Post-Vaccination Infection

Juan Carlos Andreu-Ballester, Lorena Galindo-Regal, Carmen Cuellar, Francisca López-Chuliá, Carlos García-Ballesteros, Leonor Fernández-Muga, Antonio Llombart-Cussac, María Victoria Domínguez-Márquez

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: SARS-CoV-2; vaccine; antibodies; α β T cells; γ δ T cells

Online: 17 April 2024 (08:00:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.1881.v2

Long Covid, the Gut, and Autoimmune Skin Diseases: A Novel Therapeutic Approach

Patrick W. Chambers, Shae E. Chambers

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: IFN-γ ; γ δ T cells; microbiome; d-mannose; TNF-α

Online: 28 December 2023 (14:34:12 CET)

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Preprint REVIEW | doi:10.20944/preprints202309.1467.v1

A Puzzling Mast Cell Trilogy: Anaphylaxis, MCAS and Mastocytosis

Theo Gülen

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Anaphylaxis; MCAS; mastocytosis; KIT D816V; tryptase; mediator-release symptoms; Hereditary alpha-tryptasemia; atopy

Online: 21 September 2023 (12:10:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0248.v1

Effect of Alpha Lipoic Acid Supplementation on Oxidative Stress- And Lipid Parameters in Women Diagnosed With Low-Grade Squamous Intraepithelial Lesions (LSIL)

Anja Divković, Zinaida Karasalihović, Ivana Rumora Samarin, Damir Sabitović, Kristina Radić, Nikolina Golub, Lovorka Vujić, Marija Grdić Rajković, Dubravka Vitali Čepo

Subject: Medicine And Pharmacology, Pharmacy Keywords: alpha lipoic acid; lipid status parameters; oxidative stress parameters; LSIL, diet quality index

Online: 3 August 2023 (10:16:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0170.v1

Assessment of NF-κB Inhibitor (SN50) Effect on Adipose Tumor Necrosis Factor-Alpha and Angiotensinogen Secretion and Expression

Nasser M. Al-Dagheri, Assim A. Alfadda, Reem M. Sallam, Philip G. McTernan, Lotfi S. Bin Dahman

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: abdominal subcutaneous adipocytes; angiotensinogen; nuclear factor-kappa B; lipopolysaccharide; tumor necrosis factor-alpha

Online: 6 April 2021 (11:28:00 CEST)

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Central adiposity is one of the significant determinants of obesity-related hypertension risk, which may arise due to the abdominal fat depot's pathogenic inflammatory nature. Pro-inflammatory cytokines and adipokines up-regulation through nuclear factor-kappa B (NF-κB) activation in adipose tissue has been considered an essential function in the pathogenesis of obesity-related hypertension. This study aimed to ascertain the NF-κB inhibitor (SN50) effect on TNF-α and angiotensinogen (AGT) secretion and expression in mediating the anti-inflammatory effect through its impact on NF-κB activity in humans adipose tissue. Primary human adipocytes were isolated from 20 subjects among 10 overweight and 10 obese with and without hypertension and treated with 10ng/ml LPS in the presence and absence of NF-κB inhibitor, SN50 (50μg/ml). TNF-α secretion and NF-κB p65 activity were detected in supernatants extracted from cultured cells treated and untreated with LPS (10ng/ml) and SN50 (50μg/ml) using enzyme-linked immunosorbent assay (ELISA). The western blot technique detected the protein of NF-κB p65 and AGT. Gene expression of TNF-α and AGT was detected in cells and performed using quantitative real-time polymerase chain reaction (RT-PCR). Treatment of AbdSc adipocytes with LPS (10ng/ml) caused a significant increase in NF-κB p65 among overweight and obese subjects with and without hypertension (P= 0.001) at 24 hours incubation. In contrast, SN50-NF-κB inhibitor causes a reduction of NF-κB p65 in overweight (P= <0.001) and obese subjects with and without hypertension (P= 0.001) at 24 hours incubation. Treatment of AbdSc adipocytes with 10ng/ml LPS caused a significant increase in TNF-α secretion in overweight and obese subjects at all-time points (P= <0.001), whereas SN50 leads to a decrease in TNF-α secretion at 3 and 12 hours incubation. Treatment of AbdSc adipocytes with LPS (10ng/ml) caused increased TNF-α and AGT gene expression twofold compared with untreated cells, whereas, in the presence of SN50, it reduces mRNA AGT levels in both groups. Taken together, these adipokines with NF-κB activation may represent essential biomarkers to evaluate hypertension risk and to provide insight into the pathogenesis of obesity-related hypertension.

Preprint REVIEW | doi:10.20944/preprints202103.0699.v1

Bismuth-213 for Targeted Radionuclide Therapy: From Atom to Bedside

Stephen Ahenkorah, Irwin Cassells, Christophe M Deroose, Thomas Cardinaels, Andrew R Burgoyne, Guy Bormans, Maarten Ooms, Frederik Cleeren

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Bismuth-213; Targeted Radionuclide Therapy; Targeted alpha Therapy; radiopharmaceutical; bifunctional chelator; vector molecule

Online: 29 March 2021 (14:43:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202011.0684.v1

Incretin Hormones and Type 2 Diabetes – Mechanistic Insights and Therapeutic Approaches

Geke Aline Boer, Jens Juul Holst

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: GIP; GLP-1; incretins; T2DM

Online: 27 November 2020 (11:24:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints201910.0136.v1

Preventive Effect of Cardiotrophin-1 Administration before DSS-Induced Ulcerative Colitis in Mice

Ana I Sanchez-Garrido, Vanessa Prieto-Vicente, Victor Blanco-Gozalo, Miguel Arevalo, Yaremi Quiros, Daniel Lpopez-Montañes, Francisco J. Lopez-Hernandez, Antonio Rodriguez-Perez, José M Lopez-Novoa

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: apoptosis; cardiotrophin-1; colon; inflammation

Online: 12 October 2019 (03:38:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.0389.v1

High Mobility Group Box-1 as a Candidate Biomarker of Portal Vein Thrombosis in Patients with Hepatocellular Carcinoma

Mattia Perazzi, Roberto Piffero, Rosalba Minisini, Silvia Gaia, Emanuela Rolle, Gian Paolo Caviglia, Michela Emma Burlone, Giulia Francesca Manfredi, Stelvio Tonello, Mattia Bellan, Patrizia Carucci, Mario Pirisi

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: High Mobility Group Box-1 (HMGB-1); portal vein thrombosis (PVT); hepatocellular carcinoma (HCC)

Online: 7 November 2023 (10:36:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.0876.v1

Cellular-Host Interaction of SARS-CoV-2 Gamma Variant

Aline Miranda Scovino, Elizabeth Chen Dahab, Israel Diniz-Lima, Etiele de Senna Silveira, Shana Priscila Coutinho Barroso, Karina Martins-Cardoso, Dirlei Nico, Celio Geraldo Freire-de-Lima, Leonardo Freire-de-Lima, Natalia Valente, Valeria Nacife, Ana Machado, Mia Araújo, Gustavo Fioravanti Vieira, Alex Pauvolid-Corrêa, Marilda Mendonça Siqueira, Alexandre Morrot

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: SARS-CoV-2; P.1 variant; B.1 strain; cytokines; COVID-19; macrophages; neutrophils

Online: 13 July 2023 (04:34:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0141.v1

Molecular Characterization and Antibiogram of Acinetobacter Baumannii Clinical Isolates Recovered From the Patients With Ventilator-Associated Pneumonia

Saleem Mohd, Azharuddin Sajid Syed Khaja, Ashfaq Hossain, Fahaad Alenazi, Kamaleldin B. Said, Soha Abdallah Moursi, Homoud Abdulmohsin Almalaq, Hamza Sharif Mohamed, Ehab Rakha

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Acinetobacter baumannii; XDR; IMP-1; VIM-2; NDM-1; VAP; ICU; Hospital-acquired infections

Online: 9 June 2022 (10:58:13 CEST)

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Working Paper REVIEW

Revisiting the Stress Concept in the Context of Solid Tumors Prognostic: A Role for the Stress Granules?

Anais Aulas, Pascal Finetti, Shawn Lyons, François Bertucci, Daniel Birnbaum, Claire Acquaviva, Emilie Mamessier

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: stress granules; G3BP1; G3BP2; Caprin-1; USP10; TIA1; TIAR; cancer prognosis; biomarker; metastasis; resistance; cell death; pro-survival properties

Online: 7 April 2020 (01:56:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202001.0122.v1

High PD-1 Expression Level is Associated with an Unfavorable Prognosis in Patients with Cervical Adenocarcinoma

Masako Ishikawa, Kentaro Nakayama, Kohei Nakamura, Hitomi Yamashita, Tomoka Ishibashi, Toshiko Minamoto, Kouji Iida, Sultana Razia, Noriyoshi Ishikawa, Satoru Nakayama, Yoshiro Otsuki, Satoru Kyo

Subject: Physical Sciences, Biophysics Keywords: cervical adenocarcinoma; immune-checkpoint inhibitor; programmed cell death-1(PD-1); programmed cell death-ligand 1(PD-L1); CD8 expression; lymphocyte; survival analysis

Online: 12 January 2020 (15:01:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0599.v1

Chemo-Enzymatic Synthesis of Synthons as Precursors for Enantiopure Clenbuterol and other β2- Agonists

Fredrik Heen Blindheim, Mari Bergan Hansen, Sigvart Evjen, Wei Zhu, Elisabeth Egholm Jacobsen

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: (R)-1-(4-Amino-3,5-dichlorophenyl)-2-bromoethan-1-ol; (S)-N-(2,6-dichloro-4-(1-hydroxyethyl)phenyl)acetamide; clenbuterol; ketoreductase; chiral chromatography

Online: 25 October 2018 (08:44:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1764.v1

Prolonged Inhibition of the MEK1/2-ERK Signaling Axis Primes IL-1β Expression through H3K9 Demethylation in Murine Macrophages

Rachel Low, Soon-Duck Ha, Nichita Sleapnicov, Parthiv Maneesh, Sung ouk Kim

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Macrophages; LPS; MEK1/2; ERK; IL-1β; H3K9 methylation; Cbx5; Innate immune memory; Tolerance; Priming; Training

Online: 28 August 2023 (10:12:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0944.v1

Development of Polycistronic Baculovirus Surface Display Vectors to Simultaneously Express Viral Proteins of Porcine Reproductive and Respiratory Syndrome and Analysis of Its Immunogenicity in Swine

Chao Yu Hsu, Yun Jang, Wei Ru Huang, Chi Young Wang, Hsiao Wei Wen, Pei Chien Tsai, Cheng Yao Yang, Muhammad Munir, Hung Jen Liu

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Porcine reproductive and respiratory syndrome virus; baculovirus surface display vectors; neutralizing antibody; IFN-γ; IL-4

Online: 14 August 2023 (09:48:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1539.v1

Aspergillus sclerotiorum Whole Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-Phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid

Malik Jan, Sheng-Jie Yue, Ru-Xiang Deng, Yanfang Nie, Wei Wang, Xue-Hong Zhang, Hong-Bo Hu

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Aspergillus sclerotiorum; phenazine 1-carboxylic Acid; biotransformation; 3-hydroxy phenazine 1-carboxylic acid; green chemistry

Online: 23 May 2023 (07:23:23 CEST)

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Preprint REVIEW | doi:10.20944/preprints202108.0283.v1

Recent Expeditious Growth of Type 1 Diabetes in the Gulf Arab Countries

Mohamed Jahromi, Mona Al Sheikh, Jaakko Tuomilehto

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Type 1 diabetes; human leukocyte antigen; Kuwait Type 1 Diabetes Study; Islet autoantibodies; Insulin; prediction

Online: 13 August 2021 (08:19:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0473.v1

Temporal Alpha Power in Resting-State EEG as Neuromarker of Complex Childhood Trauma in Institutionalized Adolescents

Gabriela Mariana Marcu, Ciprian Ionuț Bacilă, Ana-Maria Zăgrean

Subject: Psychiatry And Mental Health, Social Sciences Keywords: ; neuromarkers; resting-state EEG; alpha waves; complex childhood trauma; adolescents; biomarkers; adversities ;

Online: 8 May 2024 (08:49:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1829.v1

Is this the Simplest Possible Biosensor? A Surface Imprinted Polymer EIS Sensor for Detecting Alpha-synuclein, a Parkinson’s Disease Biomarker

Roslyn Massey, Rishabh Appadurai, Ravi Prakash

Subject: Chemistry And Materials Science, Biomaterials Keywords: surface imprinted polymers; electroimpedance spectroscopy; label-free biosensors; parkinson’ s disease; α -synuclein

Online: 25 January 2024 (15:47:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0918.v1

Glutathione Non-covalent Binding Sites on Hemoglobin and Major Glutathionylation Target betaCys94 Are Conservative among Both Hypoxia-Sensitive and Hypoxia-Tolerant Mammal Species

Anastasia A. Anashkina, Sergey Yurievich Simonenko, Yury L. Orlov, Irina Yu. Petrushanko

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: hypoxia; hemoglobin; glutathione; redox sensitivity; hypoxia-tolerant species; alpha subunit; beta-subunit; cysteine residues

Online: 13 December 2023 (04:59:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1891.v1

Quantitative Analysis and Molecular Docking Simulation of Flavonols from Eruca sativa Mill. And Their Effect on Skin Barrier Function

Jihye Park, Wonchul Choi, Jayoung Kim, Hye Won Kim, Jee-Young Lee, Jongsung Lee, Bora Kim

Subject: Medicine And Pharmacology, Complementary And Alternative Medicine Keywords: Eruca sativa; Skin barrier function; Peroxisome proliferator activated receptor-α ; Anti-inflammation; Docking simulation

Online: 29 November 2023 (12:16:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.0238.v1

Polymerizationof alpha,omega-Bis(o-methoxyphenyl)arenes with trans-1,4-Cyclohexanedicarboxylic acid: Yielding Organosoluble Semi-Aromatic Polyketones

Katsuya Maeyama, Ayumu Katada

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: aromatic polyketones; alpha,omega-bis(o-methoxyphenyl)arenes; Suzuki-Miyaura coupling; thermal stability; transparency

Online: 4 July 2023 (13:42:57 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202109.0133.v1

Fluid and Tissue Biomarkers of Lewy Body Dementia: Report of an LBDA Symposium

Gregory D. Scott, Moriah R. Arnold, Thomas G. Beach, Christopher H. Gibbons, Anumantha G. Kanthasamy, Russell M. Lebovitz, Afina W. Lemstra, Leslie M. Shaw, Charlotte E. Teunissen, Henrik Zetterberg, Angela S. Taylor, Todd C. Graham, Bradley F. Boeve, Stephen N. Gomperts, Neill R. Graff-Radford, Charbel E.-H. Moussa, Kathleen L. Poston, Liana S. Rosenthal, Marwan N. Sabbagh, Ryan R. Walsh, Miriam T. Weber, Melissa J. Armstrong, Jee A. Bang, Andrea C. Bozoki, Kimiko Domoto-Reilly, John E. Duda, Jori E. Fleisher, Douglas R. Galasko, James E. Galvin, Jennifer G. Goldman, Samantha K. Holden, Lawrence S. Honig, Daniel E. Huddleston, James B. Leverenz, Irene Litvan, Carol A. Manning, Karen S. Marder, Alexander Y. Pantelyat, Victoria S. Pelak, Douglas W. Scharre, Sharon J. Sha, Holly A. Shill, Mari Zoltan, Joseph F. Quinn, David J. Irwin

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: cerebrospinal fluid; alpha-synuclein; skin biopsy; seeded aggregation assays; tau, amyloid; Lewy body dementia

Online: 7 September 2021 (16:26:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0137.v1

Gross Alpha and Gross Beta Activity Concentrations in Dust Fraction of Urban Surface Deposited Sediment in Russian Cities

Mohamed Y. Hanfi, Andrian A. Seleznev, Ilia Yarmoshenko

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: urban surface deposited sediments (USDS); gross alpha activity (GA); gross beta activity (GB); dust

Online: 5 April 2021 (13:00:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0385.v1

mySORT: A Web Framework by using Deconvolution Approach to Estimating Immune Cell Composition from Complex Tissues

Shu-Hwa Chen, Bo-Yi Yu, Wen-Yu Kuo, Ya-Bo Lin, Sheng-Yao Su, I-Hsuan Lu, Chung-Yen Lin

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Cancer; Immunotherapy; Deconvolution; Alpha diversity; Beta diversity; Precision medicine; Microenvironment; Single-cell RNA sequencing

Online: 13 November 2020 (14:18:40 CET)

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Cancer immunotherapy reaches a remarkable achievement in various cancer types and brings new possibilities to improve cancer patients’ long-term survival. However, outcomes vary from case to case, and the present protocol benefits a small fraction of patients. One notable factor is the tumor microenvironment, especially the immune cell components, that may reflect the immune response's status quo on site. Thus, understanding the content of infiltrating immune cells in tumors is not only for research interesting but also a crucial subject toward precision medicine. We implement an algorithm for resolving relative proportions of twenty-one immune cell subclasses from a human tissue profiled transcriptome by microarray technology to reach the goal above. By selecting gene features and then adopting ?-Support Vector Regression, we can construct a deconvolution model and resolve the immune cell context. The excellent consistency between the estimated values and the correct immune-cell composition further demonstrates this approach provides a more natural alternative to revealing samples' immune cell content and reliable results like recent single-cell technologies. Based on this algorithm, the web-based deconvolution tool implemented named mySORT provides a user-friendly interface for estimating the immune cell content by uploading gene expression profiling. We also present comprehensive visualization 2D/3D plots in mySORT so that users can easily make a comparison between different samples. Finally, we synthesized pseudo-bulk expression data from single-cell transcriptomic datasets of 17 melanoma and 16 head and neck cancer patients. The deconvolution results of microarray-based data in the previous study and synthetic pseudo-bulk data all proved the excellent performance of mySORT. We believe that mySORT can help researchers in all fields easily understand complex immune microenvironment. The website of mySORT is freely accessible on https://symbiosis.iis.sinica.edu.tw/mySORT/.

Preprint ARTICLE | doi:10.20944/preprints202009.0251.v1

Influence of Different Light Regimes on the Mycoparasitic Activity and the Production of the Secondary Metabolite 6-Pentyl-α-Pyrone in Two Strains of Trichoderma Atroviride

Dubraska Moreno-Ruiz, Alessandro Fuchs, Kristina Missbach, Rainer Schuhmacher, Susanne Zeilinger

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Trichoderma atroviride; mycoparasitism; secondary metabolites; 6-pentyl-alpha-pyrone; Tmk3 MAP kinase; light; dark

Online: 11 September 2020 (08:49:15 CEST)

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