Preprint Article Version 1 This version is not peer-reviewed

Macrophages from Mice Administered Rhus Verniciflua Stokes Extract Show Selective Anti-Inflammatory Activity

Version 1 : Received: 29 October 2018 / Approved: 29 October 2018 / Online: 29 October 2018 (13:26:02 CET)

A peer-reviewed article of this Preprint also exists.

Kim, B.-G.; Song, Y.; Lee, M.-G.; Ku, J.-M.; Jin, S.-J.; Hong, J.-W.; Lee, S.; Kang, H. Macrophages from Mice Administered Rhus verniciflua Stokes Extract Show Selective Anti-Inflammatory Activity. Nutrients 2018, 10, 1926. Kim, B.-G.; Song, Y.; Lee, M.-G.; Ku, J.-M.; Jin, S.-J.; Hong, J.-W.; Lee, S.; Kang, H. Macrophages from Mice Administered Rhus verniciflua Stokes Extract Show Selective Anti-Inflammatory Activity. Nutrients 2018, 10, 1926.

Journal reference: Nutrients 2018, 10, 1926
DOI: 10.3390/nu10121926

Abstract

The bark of Rhus verniciflua Stokes (RVS) is used as a food additive and herbal medicine for various inflammatory disorders and cancer in Eastern Asia. RVS exerted anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, but whether this effect occurs in macrophages after oral administration has not been determined. We used a thioglycollate-induced peritonitis model to obtain macrophages from mice given RVS. We examined the systemic inflammatory response to intraperitoneal LPS. RVS-treated mice had an increased population of peritoneal exudate cells expressing CD11b and SRA. Increased uptake of Alexa Fluor 488-labeled acetylated lipoprotein was observed in monocyte-derived macrophages from RVS-treated mice. When these cells from the RVS group were stimulated with LPS, the levels of tumor necrosis factor (TNF)- and interleukin (IL)-6 in the supernatant decreased, but the level of IL-12 increased. The surface expression of CD86 was reduced, but surface expression of class II MHC molecules was increased. RVS suppressed the serum levels of LPS-induced TNF- and IL-6. RVS enhanced monocyte differentiation in thioglycollate-induced peritonitis by increasing scavenger receptor expression and activity. Macrophages isolated from mice given RVS responded differently to LPS. These findings demonstrate that RVS confers selective anti-inflammatory activity without causing the overall inhibitory effects on immune cells.

Subject Areas

Rhus verniciflua Stokes; in vivo; inflammation; macrophage; monocyte differentiation; MHC II; IL-12

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