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Aspergillus sclerotiorum Whole Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-Phenazine 1-Carboxylic Acid from Phenazine 1-Carboxylic Acid
Jan, M.; Yue, S.-J.; Deng, R.-X.; Nie, Y.-F.; Zhang, H.-Y.; Hao, X.-R.; Wang, W.; Hu, H.-B.; Zhang, X.-H. Aspergillus sclerotiorum Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic acid from Phenazine 1-Carboxylic Acid. Fermentation2023, 9, 579.
Jan, M.; Yue, S.-J.; Deng, R.-X.; Nie, Y.-F.; Zhang, H.-Y.; Hao, X.-R.; Wang, W.; Hu, H.-B.; Zhang, X.-H. Aspergillus sclerotiorum Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic acid from Phenazine 1-Carboxylic Acid. Fermentation 2023, 9, 579.
Jan, M.; Yue, S.-J.; Deng, R.-X.; Nie, Y.-F.; Zhang, H.-Y.; Hao, X.-R.; Wang, W.; Hu, H.-B.; Zhang, X.-H. Aspergillus sclerotiorum Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic acid from Phenazine 1-Carboxylic Acid. Fermentation2023, 9, 579.
Jan, M.; Yue, S.-J.; Deng, R.-X.; Nie, Y.-F.; Zhang, H.-Y.; Hao, X.-R.; Wang, W.; Hu, H.-B.; Zhang, X.-H. Aspergillus sclerotiorum Whole-Cell Biocatalysis: A Sustainable Approach to Produce 3-Hydroxy-phenazine 1-Carboxylic acid from Phenazine 1-Carboxylic Acid. Fermentation 2023, 9, 579.
Abstract
In green chemistry, filamentous fungi are regarded as a kind of robust microorganism for the biotransformation of natural products. Nonetheless, the screening of microorganisms is crucial for the effective biotransformation of natural products such as phenazine compounds. The precursor metabolite of most phenazine derivatives in Pseudomonas spp. is phenazine-1-carboxylic acid (PCA), the key constituent of shenqinmycin, widely used to control rice sheath blight in southern China. In this study, a new fungus strain Aspergillus sclerotiorum was isolated, which can efficiently convert PCA into 3-hydroxy-phenazine 1-carboxylic acid (3-OH-PCA). Moreover, an effective whole cells biotransformation system was constructed by screening optimal reaction conditions and carbon sources. Hence, Aspergillus sclerotiorum exhibited desirable adaptation by consumption of different carbon sources and maximum whole cell biomass (10.6 g/L DCW) was obtained as a biocatalyst from glucose. Optimal conditions for whole-cell biocatalysis of PCA were evaluated, including PCA concentration of 1120 mg/L, pH 7.0, temperature of 25°C, rotation rate 200 rpm and dry cell weight 15 g/L for 60 h, thus 1060 mg/L of 3-OH-PCA was obtained and the conversion efficiency of PCA was 94%. Hence, the results of repeated batch mood revealed that the biotransformation efficiency of fungus pellets reduced with each subsequent cycle but remained stable in all five cycles with the provision of glucose supplement. These findings open the prospective of using filamentous fungi for the whole cell biocatalysis of phenazine in enormous amount and efficient production of 3-OH-PCA. Moreover, these results laid the foundation for further research to disclose the genetic based mechanism of the strain responsible for PCA biotransformation.
Biology and Life Sciences, Biology and Biotechnology
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