Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Thymic Stromal Lymphopoietin promoted CD4+CD25- T cells differentiation isolated from the thymus of patients with Myasthenia Gravis to CD4+CD25+ Regulatory T Cells In Vitro

Version 1 : Received: 7 June 2020 / Approved: 7 June 2020 / Online: 7 June 2020 (15:00:37 CEST)

How to cite: Yuan, J.; Wang, N.; Karim, M.R.; Zeng, L.; Sun, Q.; Sun, Y.; Zhang, H.; Wang, Y. Thymic Stromal Lymphopoietin promoted CD4+CD25- T cells differentiation isolated from the thymus of patients with Myasthenia Gravis to CD4+CD25+ Regulatory T Cells In Vitro. Preprints 2020, 2020060093 (doi: 10.20944/preprints202006.0093.v1). Yuan, J.; Wang, N.; Karim, M.R.; Zeng, L.; Sun, Q.; Sun, Y.; Zhang, H.; Wang, Y. Thymic Stromal Lymphopoietin promoted CD4+CD25- T cells differentiation isolated from the thymus of patients with Myasthenia Gravis to CD4+CD25+ Regulatory T Cells In Vitro. Preprints 2020, 2020060093 (doi: 10.20944/preprints202006.0093.v1).

Abstract

Thymic stromal lymphopoietin (TSLP) is a cytokine and is closely related to Interleukin (IL) - 7, and hTSLP can activation through the human thymus dendritic cell in thymic to indirectly promote the differentiation of natural Regulatory T cells (Tregs) of the thymus. In this study, we focused on recombinant TSLP to determine its effects on the differentiation of CD4+CD25-T cells separated from the thymus of myasthenia gravis (MG) patients. Our results demonstrated that exogenous TSLP could increase CD4+CD25+T/CD4+T cells ratio, up-regulate the expression of Foxp3 mRNA and protein expression in CD4+CD25+Treg cells. Furthermore, we found that CD4+CD25+ Treg cells induced by exogenous TSLP could secrete IL - 10, Transforming growth factor (TGF) - β and the ability to inhibit CD4+T cell proliferation improved. These results indicate that TSLP may promote the differentiation of thymic CD4+CD25-T cells of MG patient to CD4+CD25+Foxp3+ regulatory T cells and enhance the function of immune suppression.

Subject Areas

myasthenia gravis; thymic stromal lymphopoietin; regulatory T cells; TGF- β; IL-10

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