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Preprint REVIEW | doi:10.20944/preprints202306.1863.v1

Update on Biomarkers Associated with Large Artery Atherosclerosis Stroke

Madalena Rosário, Ana Catarina Fonseca

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: atherosclerosis; stroke; carotid; stenosis; biomarker; plaque; lipid; inflammation; IL-6

Online: 27 June 2023 (11:00:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1515.v1

Additions to the Genus Helicosporium (Tubeufiaceae, Tubeufiales) from China with an Identification Key to Helicosporium Taxa

Xing-Juan Xiao, Jian Ma, Li-Juan Zhang, Ning-Guo Liu, Yuan-Pin Xiao, Xing-Guo Tian, Zong-Long Luo, Yong-Zhong Lu

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: 6 new taxa; ascomycetes; asexual morph; filamentous fungi; phylogeny; taxonomy

Online: 21 June 2023 (09:44:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0599.v1

Mechanical Characterization of Two Blends PP+PA6+EPDM. Part 2. Results of Tensile Tests

George Ghiocel Ojoc, Larisa Chiper Titire, Ana Maria Musteață, Mihail Boțan, George Catalin Cristea, Lorena Deleanu

Subject: Engineering, Automotive Engineering Keywords: polyamide 6, polypropylene, blend, EPDM, mechanical characteristic, energy at break

Online: 22 April 2021 (09:26:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201904.0298.v1

A Highly Selective Fluorescent Chemosensor for Al³⁺ Based on 2,2':6',2''-Terpyridine with a Salicylal Schiff Base

Hui Li, Jianzhi Wang, Qingyao Fu, Feng Wang, Luying He

Subject: Chemistry And Materials Science, Inorganic And Nuclear Chemistry Keywords: 2,2':6',2''-terpyridine; Salicylidene Schiff bases; Fluorescence sensor; Al3+

Online: 26 April 2019 (10:55:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201811.0605.v1

A Facile and High Selectivity Novel Fluorescence Sensing Determination of 2, 4, 6-Trinitrophenol Based on Cationic Water-Soluble Pillar[6]arene Graphene Nanocomposite

Xiaoping Tan, Tingying Zhang, Wenjie Zeng, Shuhua He, Xi Liu, Hexiang Tian, Jianwei Shi, Tuanwu Cao

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: cationic pillar[6]arene; host–guest recognition; reduced graphene; trinitrophenol

Online: 28 November 2018 (02:49:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints201807.0335.v2

Mechanical, Heat-resistant, Crystallographic and Dynamical Mechanical Properties of Nylon 6/ P(N-phenylmaleimide-alt-styrene) Blends

Yufei Liu, Min He, Daohai Zhang, Qian Zhao, Shu-Hao Qin, Jie Yu

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: Heat-resistant; Nylon 6; P(N-phenylmaleimide-alt-styrene); Blends;

Online: 16 November 2018 (15:01:35 CET)

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Preprint ARTICLE | doi:10.20944/preprints201703.0204.v1

Association Between Serum Selenium Concentrations and Levels of Proinflammatory and Profibrotic Cytokines - Interleukin-6 and Growth Differentiation Factor-15, in Patients with Alcoholic Liver Cirrhosis

Andrzej Prystupa, Paweł Kiciński, Dorota Luchowska-Kocot, Anna Blazewicz, Jarosław Niedziałek, Grzegorz Mizerski, Mariusz Jojczuk, Andrzej Ochal, Jarosław J. Sak, Wojciech Załuska

Subject: Medicine And Pharmacology, Gastroenterology And Hepatology Keywords: alcohol; liver cirrhosis; selenium; interleukin-6; growth differentiation factor-15

Online: 27 March 2017 (16:12:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0328.v1

Lonicera japonica Thunb. Ethanol Extract Exerts a Protective Effect on Normal Human Gastric Epithelial Cells by Modulating the Activity of Tumor-Necrosis-Factor-α-Induced Inflammatory Cyclooxygenase 2/Prostaglandin E2 and Matrix Metalloproteinase 9

Hsi-Lung Hsieh, Ming-Chin Yu, Yu-Chia Chang, Yi-Hsuan Wu, Kuo-Hsiung Huang, Ming-Ming Tsai

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Lonicera japonica Thunb.; Matrix metallopeptidase-9; Tumor necrosis factor-α ; Cyclooxygenase-2

Online: 4 April 2024 (12:58:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1753.v1

Heterozygous SERPINA1 Defects and Their Impact on Clinical Manifestations of Patients with Predominantly Antibody Deficiencies

Styliani Sarrou, Ioanna Voulgaridi, Athanasia Fousika, Katerina Dadouli, Olympia Margaritopoulou, Ioannis Kakkas, Christos Hadjichristodoulou, Fani Kalala, Matthaios Speletas

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Predominantly antibody deficiencies; common variable immunodeficiency; alpha-1-antitrypsin; SERPINA1; liver disease.

Online: 28 March 2024 (09:58:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.1417.v1

Phenotypic Differences among Recently Diagnosed Drug-Naïve Patients with Parkinson’s Disease with or without SNCA Polymorphisms

Santiago Pérez-Lloret, Alejandro Enet, Guenson Chevalier, Sofia Bordet, Hanny Barbar, Francisco Capani, Lucas Udovin, Matilde Otero-Losada, Francisco Barrantes

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: single nucleotide polymorphism; Parkinson’s disease; alpha-synuclein; phenotype, pathophysiology; PD-related variants

Online: 20 July 2023 (12:46:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0308.v1

Effects of Different Feeding Patterns on the Rumen Bacterial Community of Tan Lambs, based on High-Throughput Sequencing of 16S rDNA Amplicons

Lili Zhang, Yanliang Bi, Jie Zhang, Zilin Fu, Xiaofeng Xu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Alpha diversity; Beta diversity; Mutton quality; Pasture-fed; Pen-fed; Tan lambs

Online: 13 April 2023 (08:01:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202212.0276.v1

Rethinking the Paradoxical Increase in Endogenous Glucose Production in Response to Metformin: Should We Retain the Glucagon Antagonistic Effect of the Drug?

Jabeur Methnani, Taieb Ach, Aymen El Hraiech, Imed Latiri, Ali Bouslama, Monia Zaouali, Asma omezzine, Ezdine Bouhlel

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Biguanides; Antihyperglycemic agents; Liver alpha-cell axis; gluconeogenesis; Protein metabolism; insulin sensitivity

Online: 15 December 2022 (08:37:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202205.0184.v1

Amyloid Precursor Protein, Alpha-, Beta- and Gamma-Secretases Expression in Penumbra Cells after Photothrombotic Stroke. Evaluation of the Neuroprotective Effect of Secretase Inhibitors

Svetlana Sharifulina, Valeria Guzenko, Alexandr Logvinov, Andrey Khaitin, Yuliya Kalyuzhnaya, Natalia Dobaeva, Yan Li, Lei Chen, Вin He, Svetlana Demyanenko

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: amyloid precursor protein; photothrombotic stroke; ischemia; alpha-secretase; beta-secretase; gamma-secretase

Online: 13 May 2022 (08:39:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201906.0052.v1

SerThr-PhosphoProteome of Brain from Aged PINK1-KO+A53T-SNCA Mice Reveals pT1928-MAP1B and pS3781-ANK2 Deficits, as Hub between Autophagy and Synapse Changes

Georg Auburger, Suzana Gispert, Sylvia Torres-Odio, Marina Jendrach, Nadine Brehm, Júlia Canet-Pons, Jana Key, Nesli-Ece Sen

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Parkinson's disease; brain phosphorylome; PINK1, alpha-synuclein; microtubular cytoskeleton; autophagy; synaptic signaling

Online: 7 June 2019 (03:21:19 CEST)

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Hereditary Parkinson’s disease (PD) can be triggered by an autosomal dominant overdose of alpha-Synuclein (SNCA) as stressor or the autosomal recessive deficiency of PINK1 Serine/Threonine-phosphorylation activity as stress-response. We demonstrated the combination of PINK1-knockout with overexpression of SNCAA53T in double mutant (DM) mice to exacerbate locomotor deficits and to reduce lifespan. To survey posttranslational modifications of proteins underlying the pathology, brain hemispheres of old DM mice underwent quantitative label-free global proteomic mass spectrometry, focused on Ser/Thr-phosphorylations. As exceptionally strong effect, we detected >300-fold reductions of phosphoThr1928 in MAP1B, a microtubule-associated protein, and a similar reduction of phosphoSer3781 in ANK2, an interactor of microtubules. MAP1B depletion is known to trigger perturbations of microtubular mitochondria trafficking, neurite extension and synaptic function, so it was noteworthy that relevantly decreased phosphorylation was detected also for other microtubule and microfilament factors, namely MAP2S1801, MARK1S394, MAP1AT1794, KIF1AS1537, 4.1NS541, 4.1GS86 and ADD2S528. While the MAP1B heavy chain supports regeneration and growth cones, its light-chain assists DAPK1-mediated autophagy. Interestingly, relevant phosphorylation decreases of DAPK2S299, VPS13DS2429 and VPS13CS2480 in the DM brain affected regulators of autophagy, which are implicated in PD. Overall, significant downregulations were enriched for PFAM C2 domains, other kinases, and synaptic transmission factors upon automated bioinformatics, while upregulations were not enriched for selective motifs or pathways. Validation experiments confirmed the change of LC3 processing as reflection of excessive autophagy in DM brain, and dependence of ANK2/MAP1B expression on PINK1 levels. Our new data provide independent confirmation in a mouse model with combined PARK1/PARK4/PARK6 pathology that MAP1B/ANK2 phosphorylation events are implicated in Parkinsonian neurodegeneration. These findings expand on previous observations in D. melanogaster that the MAP1B ortholog futsch in the presynapse is a primary target of the PARK8 protein LRRK2, and on a report that MAP1B is a component of the pathological Lewy body aggregates in PD patient brains. Similarly, ANK2 gene locus variants are associated with the risk of PD, ANK2 interacts with PINK1/Parkin-target proteins such as MIRO1 or ATP1A2, and ANK2-derived peptides are potent inhibitors of autophagy.

Preprint ARTICLE | doi:10.20944/preprints201810.0283.v1

Geomorphology and Altitude Effects on Diversity and Structure of Vanishing Montane Forest of Southern Ecuador

Omar Cabrera, Angel Benítez, Nixon Cumbicus, Carlos Naranjo, Pablo Ramón, Fani Tinitana, Adrián Escudero

Subject: Biology And Life Sciences, Forestry Keywords: alpha diversity; beta diversity; floristic arrangement; geological landscapes; geomorphology; Ecuador; mountain forests

Online: 15 October 2018 (06:19:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0365.v1

Enantioselective Organocatalyzed a-Amination of 1,3-Dicarbonyl Compounds in Deep Eutectic Mixtures

Diego Ros Ñíguez, Pegah Khazaeli, Diego A. Alonso, Gabriela Guillena

Subject: Chemistry And Materials Science, Organic Chemistry Keywords: asymmetric organocatalysis; benzimidazole; alpha-Amination; deep eutectic solvents; green chemistry, natural products

Online: 27 April 2018 (16:23:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201709.0100.v1

Cut-off Values of Hematologic Parameters to Predict the Number of Alpha Genes Deleted in Subjects with Deletional Alpha Thalassemia

Diego Velasco-Rodríguez, Carlos Blas, Juan-Manuel Alonso-Domínguez, Gala Vega, Carlos Soto, Aránzazu García-Raso, Pilar Llamas-Sillero

Subject: Medicine And Pharmacology, Hematology Keywords: alpha; thalassemia; deletional; cut-off; number of genes; microcytic anemia; differential diagnosis

Online: 21 September 2017 (04:47:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.1280.v1

Reliability of IL-6 Alone and in Combination for Diagnosis of Late Onset Sepsis: a Systematic Review

Julia Eichberger, Elisabeth Resch, Bernhard Resch

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: interleukin-6; late-onset sepsis; sensitivity; specificity; meta-analysis; diagnostic accuracy

Online: 22 February 2024 (12:10:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0627.v1

Water, Sanitation, and Hygiene (WASH) in Schools: A Catalyst for Upholding Human Rights to Water and Sanitation in Anápolis, Brazil

C. Tonelini Pereira, Sabrina Sorlini, Josivaldo Sátiro, Antonio Albuquerque

Subject: Environmental And Earth Sciences, Other Keywords: drinking water; human rights; SDG 6; services ladders; wash in schools

Online: 12 February 2024 (04:57:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0045.v1

Interleukin-6 as a predictive factor of pathological response to FLOT regimen systemic treatment in locally advanced gastroesophageal junction (GEJ) or gastric cancer (GC) patients

Katarzyna Marcisz-Grzanka, Beata Kotowicz, Aleksandra Nowak, Mariola Winiarek, Malgorzata Fuksiewicz, Maria Kowalska, Andrzej Tysarowski, Tomasz Olesinski, Jakub Palucki, Urszula Sulkowska, Agnieszka Kolasinska-Cwikla, Lucjan Stanislaw Wyrwicz

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: gastric cancer; gastroesophageal junction cancer; IL-6; FLOT regimen; predictive biomarker

Online: 2 January 2024 (07:25:05 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1708.v1

Investigations on the Influence of Two Pyridyl-Mesoionic Carbene Constitutional Isomers on the Electrochemical and Spectroelectrochemical Properties of Group 6 Metal Carbonyl Complexes

Tobias Bens, Sarkar Biprajit

Subject: Chemistry And Materials Science, Inorganic And Nuclear Chemistry Keywords: mesoionic carbenes; (spectro)electrochemistry; carbonyl ligands; group 6 carbonyls; EPR spectroscopy

Online: 30 November 2023 (05:08:41 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.0340.v1

Insights into the Brain Effects of a Bacterial Nicotine Derivative – 6-Hydroxy-L-Nicotine

Razvan Stefan Boiangiu, Ion Brinza, Iasmina Honceriu, Marius Mihasan, Lucian Hritcu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: nicotine; metabolite; bacteria; 6-hydroxy-L-nicotine; memory; oxidative stress; degradation.

Online: 6 October 2023 (11:21:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2207.v1

Expression of Phospholipase D Family Member 6 in Bovine Testes and Its Molecular Characteristics

Rui Yang, Bo-Yang Zhang, Wen-Qian Zhu, Chun-Ling Zhu, Lan-Xin Chen, Yan-sen Zhao, Yue-Qi Wang, Yan Zhang, Amjad Riaz, Bo Tang, Xue-Ming Zhang

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Bovine; Bioinformatics; Phospholipase D family member 6; Testes; Spermatogonial stem cells

Online: 30 June 2023 (14:45:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1112.v1

Prevalence of Porcine Parvoviruses 1 to 6 and Porcine Circovirus 3 Infections and of Their Co-infections With Porcine Circovirus 2 in the Republic of Korea

In-Ohk Ouh, Ju-Yeon Lee, Hyeonhae Choi, Seo Young Moon, Jae Young Song, Bang-Hun Hyun, Dongmi Kwak, Yoon-Hee Lee, Choi-Kyu Park

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Porcine parvovirus 1-6; Porcine circovirus 2-3; Prevalence; Genetic diversity

Online: 16 May 2023 (06:02:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0287.v1

Anticholinesterase and Pro-dopaminergic Effects of Instant Coffee

Daniele O. Köhn, Graziella R. Molska, Lyvia Izaura G. Paula-Freire, Giuseppina Negri, Elisaldo A. Carlini, Fúlvio R. Mendes

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Coffea arabica; antioxidant; acetylcholinesterase inhibition; catalepsy; unilateral 6-hydroxydopamine lesion; caffeine

Online: 20 September 2022 (03:22:27 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202203.0035.v1

Biocompatible Films of Calcium Alginate Inactivate Enveloped Viruses such as SARS-CoV-2

Alba Cano-Vicent, Rina Hashimoto, Kazuo Takayama, Ángel Serrano-Aroca

Subject: Chemistry And Materials Science, Biomaterials Keywords: calcium alginate; SARS-CoV-2; bacteriophage; phi 6; biomaterials; films; hydrogels

Online: 2 March 2022 (07:17:08 CET)

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Preprint ARTICLE | doi:10.20944/preprints202106.0231.v1

Genotypic and Haplotype Analysis of Interleukin-6 and-18 Gene Polymorphisms in Association with Clinicopathological Factors in Breast Cancer

Chiranjeevi Padala, Kaushik Puranam, Nivas Shyamala, Keerthi Kupsal, Ramanjaneyulu Kummari, Rajesh Kumar Galimudi, Kishore Kumar Gundapaneni, Mohini Aiyengar Tupurani, Aparna Suryadevara, Sanjeeva Kumari CSK, Surekha Rani Hanumanth

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Breast Cancer; Haplotype; Single nucleotide polymorphisms; Interleukin-6 and Interleukin-18.

Online: 8 June 2021 (13:21:54 CEST)

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Working Paper ARTICLE

Influences on Textile and Mechanical Properties of Recycled Carbon Fiber Nonwovens Produced by Carding

Frank Manis, Georg Andreas Reinhold Stegschuster

Subject: Chemistry And Materials Science, Biomaterials Keywords: Carbon fiber; recycling; nonwoven; carding; hot pressing; Polyamide 6; Polyethylene terephthalate

Online: 27 May 2021 (07:40:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201908.0265.v1

Study of Catalytic Hydrodeoxygenation Performances of Ni/KIT-6 Catalyst－Effect of Reduction Temperature

Xianming Zhang, Shuang Chen, Fengjiao Wang, Lidan Deng, Jianmin Ren, Zhaojie Jiao, Guilin Zhou

Subject: Engineering, Energy And Fuel Technology Keywords: Mesoporous Ni/KIT-6 catalyst; Active Ni species; Catalytic hydrodeoxygenation; Biodiesel

Online: 26 August 2019 (12:34:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1115.v1

Low Number of Baselines γδ T Cells Increase the Risk of SARS-CoV-2 Post-Vaccination Infection

Juan Carlos Andreu-Ballester, Lorena Galindo-Regal, Carmen Cuellar, Francisca López-Chuliá, Carlos García-Ballesteros, Leonor Fernández-Muga, Antonio Llombart-Cussac, María Victoria Domínguez-Márquez

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: SARS-CoV-2; vaccine; antibodies; α β T cells; γ δ T cells

Online: 17 April 2024 (08:00:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.1881.v2

Long Covid, the Gut, and Autoimmune Skin Diseases: A Novel Therapeutic Approach

Patrick W. Chambers, Shae E. Chambers

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: IFN-γ ; γ δ T cells; microbiome; d-mannose; TNF-α

Online: 28 December 2023 (14:34:12 CET)

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Preprint REVIEW | doi:10.20944/preprints202309.1467.v1

A Puzzling Mast Cell Trilogy: Anaphylaxis, MCAS and Mastocytosis

Theo Gülen

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Anaphylaxis; MCAS; mastocytosis; KIT D816V; tryptase; mediator-release symptoms; Hereditary alpha-tryptasemia; atopy

Online: 21 September 2023 (12:10:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0248.v1

Effect of Alpha Lipoic Acid Supplementation on Oxidative Stress- And Lipid Parameters in Women Diagnosed With Low-Grade Squamous Intraepithelial Lesions (LSIL)

Anja Divković, Zinaida Karasalihović, Ivana Rumora Samarin, Damir Sabitović, Kristina Radić, Nikolina Golub, Lovorka Vujić, Marija Grdić Rajković, Dubravka Vitali Čepo

Subject: Medicine And Pharmacology, Pharmacy Keywords: alpha lipoic acid; lipid status parameters; oxidative stress parameters; LSIL, diet quality index

Online: 3 August 2023 (10:16:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0170.v1

Assessment of NF-κB Inhibitor (SN50) Effect on Adipose Tumor Necrosis Factor-Alpha and Angiotensinogen Secretion and Expression

Nasser M. Al-Dagheri, Assim A. Alfadda, Reem M. Sallam, Philip G. McTernan, Lotfi S. Bin Dahman

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: abdominal subcutaneous adipocytes; angiotensinogen; nuclear factor-kappa B; lipopolysaccharide; tumor necrosis factor-alpha

Online: 6 April 2021 (11:28:00 CEST)

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Central adiposity is one of the significant determinants of obesity-related hypertension risk, which may arise due to the abdominal fat depot's pathogenic inflammatory nature. Pro-inflammatory cytokines and adipokines up-regulation through nuclear factor-kappa B (NF-κB) activation in adipose tissue has been considered an essential function in the pathogenesis of obesity-related hypertension. This study aimed to ascertain the NF-κB inhibitor (SN50) effect on TNF-α and angiotensinogen (AGT) secretion and expression in mediating the anti-inflammatory effect through its impact on NF-κB activity in humans adipose tissue. Primary human adipocytes were isolated from 20 subjects among 10 overweight and 10 obese with and without hypertension and treated with 10ng/ml LPS in the presence and absence of NF-κB inhibitor, SN50 (50μg/ml). TNF-α secretion and NF-κB p65 activity were detected in supernatants extracted from cultured cells treated and untreated with LPS (10ng/ml) and SN50 (50μg/ml) using enzyme-linked immunosorbent assay (ELISA). The western blot technique detected the protein of NF-κB p65 and AGT. Gene expression of TNF-α and AGT was detected in cells and performed using quantitative real-time polymerase chain reaction (RT-PCR). Treatment of AbdSc adipocytes with LPS (10ng/ml) caused a significant increase in NF-κB p65 among overweight and obese subjects with and without hypertension (P= 0.001) at 24 hours incubation. In contrast, SN50-NF-κB inhibitor causes a reduction of NF-κB p65 in overweight (P= <0.001) and obese subjects with and without hypertension (P= 0.001) at 24 hours incubation. Treatment of AbdSc adipocytes with 10ng/ml LPS caused a significant increase in TNF-α secretion in overweight and obese subjects at all-time points (P= <0.001), whereas SN50 leads to a decrease in TNF-α secretion at 3 and 12 hours incubation. Treatment of AbdSc adipocytes with LPS (10ng/ml) caused increased TNF-α and AGT gene expression twofold compared with untreated cells, whereas, in the presence of SN50, it reduces mRNA AGT levels in both groups. Taken together, these adipokines with NF-κB activation may represent essential biomarkers to evaluate hypertension risk and to provide insight into the pathogenesis of obesity-related hypertension.

Preprint REVIEW | doi:10.20944/preprints202103.0699.v1

Bismuth-213 for Targeted Radionuclide Therapy: From Atom to Bedside

Stephen Ahenkorah, Irwin Cassells, Christophe M Deroose, Thomas Cardinaels, Andrew R Burgoyne, Guy Bormans, Maarten Ooms, Frederik Cleeren

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Bismuth-213; Targeted Radionuclide Therapy; Targeted alpha Therapy; radiopharmaceutical; bifunctional chelator; vector molecule

Online: 29 March 2021 (14:43:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1012.v1

A New Quinazolinone Alkaloid from Rhodiola tibetica Endophytic Fungus Penicillium sp. HJT-A-6

Dongliang Xiao, Yan Wang, Congcong Gao, Xuemei Zhang, Weixing Feng, Xuan Lu, Baomin Feng

Subject: Chemistry And Materials Science, Other Keywords: Rhodiola tibetica; Penicillium sp. HJT-A-6, quinazolinone, seed-germination-promoting activity

Online: 16 April 2024 (11:02:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202311.1592.v1

Methyl-Beta-Cyclodextrin Restores Aberrant Bone Morphogenetic Protein 2-Signaling in Bone Marrow Stromal Cells Obtained from Aged C57BL/6 Mice

Daniel Halloran, Venu Pandit, Kelechi Chukwuocha, Anja Nohe

Subject: Biology And Life Sciences, Aging Keywords: osteoporosis; osteoblasts; BMP-2; BMPRIa; C57BL/6 mice; MβCD; QDot®s

Online: 24 November 2023 (09:31:34 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.1483.v1

Breast Cancer Treatment: To tARget or Not? That is the Question

Alexandra Stone, Kevin Lin, Ghanshyam H. Ghelani, Sanik Patel, Sam Benjamin, Stephen Graziano, Leszek Kotula

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: AR; TNBC; CDK4/6; CYP17 lyase; PI3K/AKT; ER; PR; HER2; DHT

Online: 24 October 2023 (05:51:15 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202309.1344.v1

Zoon’s Balanitis as a Separate Type of Balanitis with the Characteristic Pattern of Pro-Inflammatory Cytokines Expression

Mateusz Czajkowski, Katarzyna Czajkowska, Piotr M. Wierzbicki, Anna Kotulak-Chrząszcz, Małgorzata Sokołowska-Wojdyło, Marzena Kogut-Wierzbicka, Zbigniew Kmieć, Marcin Matuszewski

Subject: Medicine And Pharmacology, Urology And Nephrology Keywords: zoon balanitis; IL‑1; IL‑1RN; IL‑6; IFN‑γ; TGF‑β1

Online: 20 September 2023 (05:42:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1766.v1

6-Lead Electrocardiography is a Viable Option for Identification of Rhythm and Conduction Anomalies of Patients in the Telemedicine-Based, Hospital-at-Home Setting. A Prospective Validation Study

Adam Sharabi, Eli Abutbul, Eitan Grossbard, Yonatan Martsiano, Aya Berman, Reut Kassif-Lerner, Hila Hakim, Pninit Liber, Anram Zoubi, Galia Barkai, Gad Segal

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Electrocardiography; Hospital at home; Arrythmia; ECG Intervals; Electrolyte disturbances; 6 Lead ECG

Online: 28 August 2023 (10:26:47 CEST)

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Abstract: Background: The Hospital-at-home (HAH) model is a viable alternative for conven-tional in-hospital stays worldwide. Serum electrolyte abnormalities are common in acute pa-tients, especially in those with many comorbidities. Pathologic changes in cardiac electrophysi-ology pose a potential risk during HAH stay. Periodical Electrocardiogram (ECG) tracing is therefore advised, but few studies evaluated the accuracy and efficiency of compact, self-activated ECG devices in the HAH settings. This study aimed to evaluate the reliability of such a device in comparison to a standard 12-lead ECG. Methods: We prospectively recruited consecu-tive patients admitted to the Sheba Beyond, virtual hospital, in the HAH department, during a 3-month duration. Each patient underwent a 12-lead ECG recording using the legacy device, and a consecutive recording by a compact 6-lead device. Baseline patients’ characteristics during hospi-talization were collected. Level of agreement between devices was measured by Cohen’s Kappa coefficient for inter-rater reliability (Ϗ). Results: Fifty patients were included in the study. 26 (52%) had electrolyte disturbances. Abnormal D-dimer values were observed in 33 (66%) pa-tients, and 12 (24%) patients had elevated troponin values. We found a level of 94.5% raw agreement between devices with regards to nine of the options included in the automatic read-out of the legacy device. The calculated Ϗ was 0.72, classified as substantial consensus. The rate of raw consensus regarding ECG intervals’ measurement (PR, RR, QT) was 78.5% and the calculated Ϗ was 0.42, corresponding to a moderate level of agreement. Conclusion: This is the first report to our knowledge regarding the feasibility of using a compact, 6-Lead ECG device in the setting of HAH to be safe and bearing satisfying agreement level with a legacy, 12-lead ECG device, en-abling quick, accessible arrythmia detection in this setting. Our findings bear a promise to the future development of telemedicine-based hospital at home methodology.

Preprint ARTICLE | doi:10.20944/preprints202307.0328.v1

Tuning of Structural, Morphological and Optical Properties of Pa 6 upon Stepwise Insertion of Amorphous Se, Cd2+ and Ag+ Ions

Emilija Skuodaitė, Valentina Krylova

Subject: Chemistry And Materials Science, Surfaces, Coatings And Films Keywords: polyamide 6; selenium; cadmium; silver; selenides; nanofilms; optical properties; light-absorbing materials

Online: 6 July 2023 (07:48:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1131.v1

Inflammatory Biomarker Profiles in Very Preterm Infants within the Context of Preeclampsia, Chorioamnionitis and Clinically Diagnosed Postnatal Infection

Jordan T. Ewald, Baiba Steinbrekera, Jennifer R. Bermick, Donna A. Santillan, Tarah T. Colaizy, Mark K. Santillan, Robert D. Roghair

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: adipokine; chemokine (C-C motif) ligand 2; interleukin-6; leptin; neonatal; preeclampsia

Online: 16 May 2023 (09:00:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202111.0049.v1

C57BL/6 Background Attenuates mHTT Toxicity in the Striatum of YAC128 Mice

Michaela Kerstin Back, Johanna Kurzawa, Sonia Ruggieri, Jakob von Engelhardt

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Huntington’s disease; YAC128; HdhQ150; strain background; C57BL/6; synaptic pathology; extrasynaptic NMDAR

Online: 2 November 2021 (12:11:26 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202103.0054.v1

DRESS Syndrome in a Teenage Male Associated With Hhv-6 Reactivation While on Prolonged TMP-SMX Use

Dalia Conteras, Iris Pecson, Alvaro Galvis

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: DRESS syndrome, Eosinophilia, Adverse drug reaction, HHV-6 reactivation, Bactrim adverse reaction

Online: 2 March 2021 (09:26:48 CET)

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Preprint REVIEW | doi:10.20944/preprints202008.0020.v1

SARS-CoV-2 and Covid-19 Immunopathogenesis

Antonio Luiz Boechat, Beatriz Pessoa, Carlos Soares, Cecília Barroso, David Vila, Emanuelly Barbosa, Isabela Seffair, João Victor Melo, Julia Becil, Maria Polyanna Rebouças, Natascha Rodrigues, Pedro Henrique Freitas, Rebeka Rocha, Thaise Rodrigues, Vanessa Ferreira, Rosmery Ubiera, Maria Cristina Dos-Santos

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: SARS-CoV-2; COVID-19; Immunopathogenesis; Cytokine storm; IL-6; macrophages; neutrophils

Online: 2 August 2020 (11:53:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202002.0038.v1

Changes in DNA Methylation in Response to 6-Benzylaminopurine Affect Allele-Specific Gene Expression in Populus tomentosa

Anran Xuan, Yuepeng Song, Chenhao Bu, Panfei Chen, Yousry A. El-Kassaby, Deqiang Zhang

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: allele specific expression; 6-BA; DNA methylation; long noncoding RNA; siRNA; poplar

Online: 4 February 2020 (05:22:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints201909.0175.v1

Decentralized Water Purification Using Novel Membrane Filtration Technology Approach to Improve Community Health

Jochen G. Raimann, Joseph Marfo Boaheng, Phillip Narh, Seth Johnson, Linda Donald, Hongbin Zhang, Friedrich Port, Nathan W. Levin

Subject: Biology And Life Sciences, Food Science And Technology Keywords: water purification; developing countries; SDG-6; microbiological contamination; public health; membrane filtration

Online: 16 September 2019 (17:23:23 CEST)

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Introduction: In rural communities in regions with limited resources the provision of clean water remains challenging. Fecal contamination of water is very common and results in a high incidence of diarrhea, subsequent acute kidney injury and mortality particularly in the very young and old. Membrane filtration is a practical solution to this problem and recent innovation allows membrane filtration using recycled hemodialyzers. We, Easy Water for Everyone, have quantified the systematic effect on health outcomes. Material and Methods: Between 02/2018 and 12/2018, 4 communities in rural Ghana (in the Greater-Accra region) were each provided with a high-volume membrane filtration devices (NUF 500; NuFiltration using recycled hemodialyzers). Health data from montly household surveys and chart review in local healthcare facilities were collected with approval from Ghana Health Services. Specifically, data was collected on gastrointestinal disease, acute kidney injury and therapeutic interventions. Incidence rates for a five-months period before and after implementation of the device were calculated and compared to rates during the same months from 4 neighboring communities that were not yet provided with the device. Results: Acceptance of the devices and the purified water in the studied villages was good and self-reported data of 1130 villagers over 10 months from 9 studied communities in rural Ghana (11% younger than 5 years and 14 % older than 65 years) were included in this analysis. The overall monthly incidence rate of diarrhea showed a decline following the implementation of the device in the 4 study villages from a mean of 0.18 to 0.05 cases per person-month for a reduction in rates by 72% (rate ratio = 0.27). By contrast, the control group of 4 villages in the same region showed no decline in mean rates during the same months as the study period with mean rates changing not significantly from 0.11 to 0.08 cases per person-month. Discussion: Provision of a hemodialyzer membrane filtration device markedly improves health outcomes as measured by diarrhea incidence within rural communities. While our data awaits confirmation in a larger population and further statistical analyses accounting for village characteristics, seasonality and subject demographics, the obvious decline in incidence rates supports widespread use of hemodialyzer membrane filtration devices, particularly in rural regions. Rollout of the device in further sites will likely increase our understanding in terms of risk and other preventive factors modifying the incidence of diarrhea and subsequent acute kidney injury.

Preprint REVIEW | doi:10.20944/preprints201905.0040.v1

Molecular Mechanisms of Cancer-Induced Sleep Disruption

William H. Walker II, Jeremy C. Borniger

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: breast cancer; sleep; IL-6; hypocretin/orexin; leptin; EEG; autonomic nervous system

Online: 6 May 2019 (08:52:31 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints201810.0136.v2

Fluorescence and Naked-Eye Detection of Pb²⁺in Drinking Water Using a Low-Cost Ionophore Based Sensing Scheme

Aron Hakonen, Niklas Strömberg

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: lead ions; fluorescence detection; ionophore; Benzo-18-Crown-6-ether; drinking water

Online: 30 October 2018 (07:42:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1829.v1

Is this the Simplest Possible Biosensor? A Surface Imprinted Polymer EIS Sensor for Detecting Alpha-synuclein, a Parkinson’s Disease Biomarker

Roslyn Massey, Rishabh Appadurai, Ravi Prakash

Subject: Chemistry And Materials Science, Biomaterials Keywords: surface imprinted polymers; electroimpedance spectroscopy; label-free biosensors; parkinson’ s disease; α -synuclein

Online: 25 January 2024 (15:47:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0918.v1

Glutathione Non-covalent Binding Sites on Hemoglobin and Major Glutathionylation Target betaCys94 Are Conservative among Both Hypoxia-Sensitive and Hypoxia-Tolerant Mammal Species

Anastasia A. Anashkina, Sergey Yurievich Simonenko, Yury L. Orlov, Irina Yu. Petrushanko

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: hypoxia; hemoglobin; glutathione; redox sensitivity; hypoxia-tolerant species; alpha subunit; beta-subunit; cysteine residues

Online: 13 December 2023 (04:59:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1891.v1

Quantitative Analysis and Molecular Docking Simulation of Flavonols from Eruca sativa Mill. And Their Effect on Skin Barrier Function

Jihye Park, Wonchul Choi, Jayoung Kim, Hye Won Kim, Jee-Young Lee, Jongsung Lee, Bora Kim

Subject: Medicine And Pharmacology, Complementary And Alternative Medicine Keywords: Eruca sativa; Skin barrier function; Peroxisome proliferator activated receptor-α ; Anti-inflammation; Docking simulation

Online: 29 November 2023 (12:16:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202307.0238.v1

Polymerizationof alpha,omega-Bis(o-methoxyphenyl)arenes with trans-1,4-Cyclohexanedicarboxylic acid: Yielding Organosoluble Semi-Aromatic Polyketones

Katsuya Maeyama, Ayumu Katada

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: aromatic polyketones; alpha,omega-bis(o-methoxyphenyl)arenes; Suzuki-Miyaura coupling; thermal stability; transparency

Online: 4 July 2023 (13:42:57 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202109.0133.v1

Fluid and Tissue Biomarkers of Lewy Body Dementia: Report of an LBDA Symposium

Gregory D. Scott, Moriah R. Arnold, Thomas G. Beach, Christopher H. Gibbons, Anumantha G. Kanthasamy, Russell M. Lebovitz, Afina W. Lemstra, Leslie M. Shaw, Charlotte E. Teunissen, Henrik Zetterberg, Angela S. Taylor, Todd C. Graham, Bradley F. Boeve, Stephen N. Gomperts, Neill R. Graff-Radford, Charbel E.-H. Moussa, Kathleen L. Poston, Liana S. Rosenthal, Marwan N. Sabbagh, Ryan R. Walsh, Miriam T. Weber, Melissa J. Armstrong, Jee A. Bang, Andrea C. Bozoki, Kimiko Domoto-Reilly, John E. Duda, Jori E. Fleisher, Douglas R. Galasko, James E. Galvin, Jennifer G. Goldman, Samantha K. Holden, Lawrence S. Honig, Daniel E. Huddleston, James B. Leverenz, Irene Litvan, Carol A. Manning, Karen S. Marder, Alexander Y. Pantelyat, Victoria S. Pelak, Douglas W. Scharre, Sharon J. Sha, Holly A. Shill, Mari Zoltan, Joseph F. Quinn, David J. Irwin

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: cerebrospinal fluid; alpha-synuclein; skin biopsy; seeded aggregation assays; tau, amyloid; Lewy body dementia

Online: 7 September 2021 (16:26:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0137.v1

Gross Alpha and Gross Beta Activity Concentrations in Dust Fraction of Urban Surface Deposited Sediment in Russian Cities

Mohamed Y. Hanfi, Andrian A. Seleznev, Ilia Yarmoshenko

Subject: Environmental And Earth Sciences, Atmospheric Science And Meteorology Keywords: urban surface deposited sediments (USDS); gross alpha activity (GA); gross beta activity (GB); dust

Online: 5 April 2021 (13:00:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202011.0385.v1

mySORT: A Web Framework by using Deconvolution Approach to Estimating Immune Cell Composition from Complex Tissues

Shu-Hwa Chen, Bo-Yi Yu, Wen-Yu Kuo, Ya-Bo Lin, Sheng-Yao Su, I-Hsuan Lu, Chung-Yen Lin

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Cancer; Immunotherapy; Deconvolution; Alpha diversity; Beta diversity; Precision medicine; Microenvironment; Single-cell RNA sequencing

Online: 13 November 2020 (14:18:40 CET)

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Cancer immunotherapy reaches a remarkable achievement in various cancer types and brings new possibilities to improve cancer patients’ long-term survival. However, outcomes vary from case to case, and the present protocol benefits a small fraction of patients. One notable factor is the tumor microenvironment, especially the immune cell components, that may reflect the immune response's status quo on site. Thus, understanding the content of infiltrating immune cells in tumors is not only for research interesting but also a crucial subject toward precision medicine. We implement an algorithm for resolving relative proportions of twenty-one immune cell subclasses from a human tissue profiled transcriptome by microarray technology to reach the goal above. By selecting gene features and then adopting ?-Support Vector Regression, we can construct a deconvolution model and resolve the immune cell context. The excellent consistency between the estimated values and the correct immune-cell composition further demonstrates this approach provides a more natural alternative to revealing samples' immune cell content and reliable results like recent single-cell technologies. Based on this algorithm, the web-based deconvolution tool implemented named mySORT provides a user-friendly interface for estimating the immune cell content by uploading gene expression profiling. We also present comprehensive visualization 2D/3D plots in mySORT so that users can easily make a comparison between different samples. Finally, we synthesized pseudo-bulk expression data from single-cell transcriptomic datasets of 17 melanoma and 16 head and neck cancer patients. The deconvolution results of microarray-based data in the previous study and synthetic pseudo-bulk data all proved the excellent performance of mySORT. We believe that mySORT can help researchers in all fields easily understand complex immune microenvironment. The website of mySORT is freely accessible on https://symbiosis.iis.sinica.edu.tw/mySORT/.

Preprint ARTICLE | doi:10.20944/preprints202001.0295.v1

Suppression of Hepatocyte Nuclear Factor 4 Alpha by Long-Term Infection of Hepatitis B Virus Contributes to Tumor Cell Proliferation

Soree Park, Yea Na Ha, Mehrangiz Dezhbord, Ah Ram Lee, Eun-Sook Park, Yong Kwang Park, Juhee Won, Na Yeon Kim, Soo Yeun Choo, Jae Jin Shin, Chang Hyun Ahn, Kyun-Hwan Kim

Subject: Biology And Life Sciences, Virology Keywords: Hepatitis B virus; hepatocyte nuclear factor 4 alpha; long-term infection; ERK signaling pathway

Online: 25 January 2020 (15:25:57 CET)

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Preprint ARTICLE | doi:10.20944/preprints201809.0058.v1

Integrative Proteomic and Metabolomic Analysis Reveals the Cotton Plant Defense Mechanisms Induced by Insect (Adelphocoris suturalis Jakovlev) Feeding

Hui Lu, Shuai Zhang, Junyu Luo, Chunyi Wang, Limin Lv, Lijuan Zhang, Xiangzhen Zhu, Li Wang, Xueke Gao, Hongxia Hua, Jinjie Cui

Subject: Biology And Life Sciences, Plant Sciences Keywords: Gossypium hirsutum L.; Adelphocoris suturalis (Jakovlev); alpha-linolenic acid pathway; tryptophan metabolism; plant defence

Online: 4 September 2018 (06:29:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201710.0180.v1

Low Levels of IgG Recognizing the Alpha-1-Antitrypsin (A1AT) ^50-63 Peptide and Its Association with Taiwanese Women with Primary Sjögren's Syndrome

Yu-Sheng Chang, Chih-Hong Pan, Che-Chang Chang, Kai-Leun Tsai, Han-Wen Chou, Jin-Hua Chen, Sheng-Hong Lin, Yi-Ying Lu, Chih-Chun Tai, Yi-Fang Lin, Ching-Yu Lin

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: primary Sjögren's syndrome; alpha-1-antitrypsin; inhibitor; 4-hydroxy-2-nonenal; autoantibody isotypes; serum

Online: 30 October 2017 (04:30:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1740.v1

Tocilizumab Exerts Anti-tumor Effects on Proliferation, Invasiveness, ERK 1/2 and STAT3 Signaling Transduction in Colorectal Carcinoma Cell Xenografts Corresponding to Expression Levels of IL-6 Receptors

Yuan-Chiang Chung, Szu-Jung Chen, Yuan-Chiang Chung, Wei-Chun Liu, Ming-Tsung Lai, Ting-Yu Kao, Wei-Shun Yang, Chien-Hui Yang, Chih-Ping Hsu, Jia-Feng Chang

Subject: Biology And Life Sciences, Life Sciences Keywords: Interleukin-6 receptor; colorectal carcinoma; xenograft; Tocilizumab; Ki-67; ERK 1/2; STAT3

Online: 28 November 2023 (03:43:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1479.v1

Interplay of Serum Leptin, Tumor Necrosis Factor Alpha, and Testosterone in Overweight-Related Hypertensive Men

Shalan Alaamri, Abdulhalim S Serafi, Zahir Hussain, Shouq K. Bafail, Mohammed A. Bafail, Lusine H. Demirkhanyan, Christopher S Gondi, Sumera Sohail

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Hypertension; overweight-related hypertension; serum leptin; TNF-α, testosterone; total cholesterol; IL-6

Online: 21 June 2023 (08:43:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0987.v1

Iguratimod Ameliorates the Severity of Secondary Progressive Multiple Sclerosis in Model Mice by Directly Inhibiting IL-6 Production and Th17 Cell Migration via Mitigation of Glial Inflammation

Satoshi Nagata, Ryo Yamasaki, Ezgi Ozdemir Takase, Kotaro Iida, Mitsuru Watanabe, Katsuhisa Masaki, Marion Heleen Cathérine Wijering, Hiroo Yamaguchi, Jun-ichi Kira, Noriko Isobe

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: iguratimod; secondary progressive multiple sclerosis; connexin 47; experimental autoimmune encephalomyelitis; IL-6; astrocyte

Online: 14 June 2023 (04:59:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1642.v1

Structural Characterization and Anticancer Activity of a New Anthraquinone from Senna velutina

David Tsuyoshi Hiramatsu Castro, Daniel Ferreira Leite, Debora da Silva Baldívia, Helder Freitas Dos Santos, Sikiru Olaitan Balogun, Denise Brentan Silva, Carlos Alexandre Carollo, Kely De Picoli Souza, Edson Lucas dos Santos

Subject: Biology And Life Sciences, Life Sciences Keywords: melanoma 1; keyword 2; leukemia 3; medicinal plants 4; anthracene 5; caspase 6

Online: 23 May 2023 (10:44:52 CEST)

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Cancer is a complex disease, considered a major public health problem worldwide. Among the types of cancer, melanoma, and leukemias present high mortality rates in Brazil and worldwide. Currently, conventional cytotoxic treatments cause severe side effects by the non-selectivity between normal cells and cancer cells. Therefore, molecules of natural origin with more efficient anticancer properties and that present fewer adverse effects are of extreme importance for cancer therapy and improvement in patients’ quality of life. This study isolated, identified, and characterized the chemical structure of a new anthraquinone present in the extract of the roots of Senna velutina. In addition, we sought to evaluate the anticancer potential of this molecule against melanoma and leukemic cell lines and identify the pathways of cell death involved. To this end, a novel anthraquinone was isolated from the barks of the roots of S. velutina, analyzed by HPLC-DAD, and its molecular structure was determined by NMR. Subsequently, their cytotoxic activity was evaluated by the MTT method against non-cancerous, melanoma, and leukemic cells. The migration of melanoma cells was evaluated by the scratch assay. By flow cytometry technique, the apoptosis process, caspase-3 activation, analysis of mitochondrial membrane potential, and measurement of ROS were evaluated. In addition, the pharmacological cell death inhibitors NEC-1, RIP-1, BAPTA, Z-VAD, and Z-DEVD were used to confirm the related cell death mechanisms. With the results, it was possible to elucidate the novel compound characterized as 2'-OH-Torosaol I. In normal cells, the compound showed no cytotoxicity in PBMC but reduced the cell viability of all melanoma and leukemic cell lines evaluated. 2'-OH-Torosaol I inhibited chemotaxis of B16F10-Nex2, SK-Mel-19, SK-Mel28 and SK-Mel-103. The cytotoxicity of the compound was induced by apoptosis via the intrinsic pathway with reduced mitochondrial membrane potential, increased levels of reactive oxygen species, and activation of caspase-3. In addition, the inhibitors demonstrated the involvement of necroptosis and CA+ in the death process and confirmed caspase-dependent apoptosis death as one of the main programmed-cell death pathways induced by 2'-OH-Torosaol I. Taken together, the data characterize the novel anthraquinone 2'-OH-Torosaol I, demonstrating its anticancer activity and potential application in cancer therapy.

Preprint ARTICLE | doi:10.20944/preprints202211.0273.v1

Pantothenate and L-carnitine Supplementation Corrects Pathological Alterations in Cellular Models of KAT6A Syndrome

Manuel Munuera-Cabeza, Mónica Álvarez-Córdoba, Juan M. Suárez-Rivero, Suleva Povea-Cabello, Irene Villalón-García, Marta Talaverón-Rey, Alejandra Suárez-Carrillo, Diana Reche-López, Paula Cilleros-Holgado, Rocío Piñero-Pérez, José A. Sánchez-Alcázar

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: intellectual disability; KAT6A syndrome; Lysine acetyltransferase 6 A; pantothenate; L-carnitine; histone acetylation

Online: 15 November 2022 (04:14:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0206.v1

Highly Accurate Pose Estimation as Reference for Autonomous Vehicles in Near-Range Scenarios

Ursula Kälin, Louis Staffa, David Eugen Grimm, Axel Wendt

Subject: Engineering, Control And Systems Engineering Keywords: Motion capture camera; robotic total station; autonomous vehicle; 6 DoF pose estimation; accuracy

Online: 13 December 2021 (13:30:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202103.0710.v1

Increased Angiotensin-Converting Enzyme 2, sRAGE and iMmune Activation, but Lowered Calcium and Magnesium In COVID-19: Association With Chest CT Abnormalities and Lowered Peripheral Oxygen Saturation

Hussein Al-Hakeim, Hawraa Al-Jassas, Gerwyn Morris, Michael Maes

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: COVID-19; sRAGE; ACE2; inflammation; immune; oxidative stress; IL-6; IL-10; biomarkers

Online: 29 March 2021 (17:03:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202103.0139.v1

ACTH(6-9)PGP Peptide Protects SH-SY5Y Cells from the H2O2, tert-Butyl Hydroperoxide, and Cyanide Cytotoxicity via Prolif-eration Stimulation and Antioxidant-related Genes Induction

Mikhail G. Akimov, Elena V. Fomina-Ageeva, Polina V. Dudina, Ludmila A. Andreeva, Nikolay F. Myasoyedov, Vladimir V. Bezuglov

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: ACTH(6-9); Neuroprotection; MPP+; H2O2; tert-butyl hydroperoxide; cyanide; melanocortins; oxidative stress

Online: 3 March 2021 (16:49:08 CET)

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Preprint REVIEW | doi:10.20944/preprints201808.0044.v1

BRK”ing” Down All We Know About PTK6 in Breast Cancer

Yi Yuan, Hui Li Ang, Xianning Lai, Lingzhi Wang, Vijay Pandey, Benjamin BoJun Huang, Tuan Zea Tan, Hui Yi Chong, Philip Zheng Yang Ong, Kanchugarakoppal S. Rangappa, Frank Arfuso, Kevin Shyong-Wei Tan, Basappa, Boon Cher Goh, Ruby Yun-Ju Huang, Gautam Sethi, Peter E. Lobie, Alan Prem Kumar

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: breast tumor kinase (BRK); protein tyrosine kinase 6 (PTK6); breast cancer; pathways; therapeutics

Online: 2 August 2018 (09:58:55 CEST)

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Preprint CONCEPT PAPER | doi:10.20944/preprints201804.0115.v1

Omega-3 Fatty Acid Supplementation—A Possible Dietary Adjunct to Enhance Immune Therapy in Cancer?

Nikolaus Wachtel, Nadine Rohwer, Anne Pietzner, Andreas Loew, Karsten H. Weylandt

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: omega-3 and omega-6 polyunsaturated fatty acids; colorectal cancer; cancer immune therapy

Online: 10 April 2018 (08:05:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0641.v1

Structure-Activity Relationship of Synthetic Linear KTS-Peptides Containing Meta-Aminobenzoic Acid as Antagonists of α1β1 Integrin with Antiangiogenic and Melanoma’ Antitumor Activities

Majdi Saleem Naamneh, Tatjana Momic, Michal Klazas, Julius Grosche, Johannes A Eble, Cezary Marcinkiewicz, Netaly Khazanov, Hanoch Senderowitz, Amnon Hoffman, Chaim Gilon, Jehoshua Katzhendler, Philip Lazarovici

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: MABA; KTS; peptide synthesis; modeling; conformation; stability; integrin α 1; cell adhesion; angiogenesis; melanoma tumor

Online: 9 April 2024 (09:51:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.1365.v1

Role of the Alpha-B-Crystallin Protein in Cardiomyopathic Disease

Andres Thorkelsson, Michael T. Chin

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: alpha-B-crystallin; cryab; molecular chaperone; desminopathy; hypertrophic cardiomyopathy; dilated cardiomyopathy; restrictive cardiomyopathy; calcineurin; NFAT

Online: 18 January 2024 (10:30:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0375.v1

Gold(I) N-heterocyclic Carbene Complexes with 7-Azaindoles Demonstrate In Vitro Antiproliferative Effects on Ovarian Cancer Cells and Anti-inflammatory Activity

Zdeněk Trávníček, Ján Vančo, Jan Belza, Michal Čajan, Jan Hošek, Zdeněk Dvořák

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: gold(I) complex; 7-azaindoles; anticancer; anti-inflammatory; cell cycle; apoptosis.; TNF-alpha; NF-kappaB

Online: 4 January 2024 (09:28:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202303.0230.v1

Attenuation of Dopaminergic Neurodegeneration in a C. elegans Parkinson’s Model through Regulation of Xanthine Dehydrogenase (XDH-1) Expression by the RNA Editase, ADR-2

Lindsey A. Starr, Luke E. McKay, Kylie N. Peter, Lena M. Seyfarth, Laura A. Berkowitz, Kim A. Caldwell, Guy A. Caldwell

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: alpha-synuclein; ADAR; C. elegans; dopamine; neurodegeneration; Parkinson's disease; RNA editing; xanthine dehydrogenase; ABC transporter

Online: 13 March 2023 (10:34:06 CET)

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Preprint REVIEW | doi:10.20944/preprints202211.0448.v1

COVID-19 Pathology Sheds New Light on Balance between Neutrophil Proteases and Their Inhibitors

Marko Radic, Vasuki Vindiya Silva

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: alpha-1-antitrypsin (AAT); neutrophil elastase (NE); NETosis, PAD4, ARDS, COPD; neutrophils; COVID-19; TMPRSS2.

Online: 24 November 2022 (02:50:25 CET)

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Preprint ARTICLE | doi:10.20944/preprints202209.0197.v1

Alpha-Synuclein at the Interface between Depression, Parkinson’s Disease and Dementia: Evidence from Epidemiology, Population Genetics and Gene-Environment Interactions

Ravi Philip Rajkumar

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: alpha-synuclein; SNCA; major depression; dysthymia; Parkinson’s disease; dementia; neurodegeneration; gene-environment interaction; PM2.5; pesticides

Online: 14 September 2022 (08:38:38 CEST)

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Preprint REVIEW | doi:10.20944/preprints202207.0100.v1

From Minor Mutant to Dominance, the Evolution of SARS-CoV-2 Variants

Thomas Caspari

Subject: Biology And Life Sciences, Virology Keywords: omicron; alpha; delta; deltacron; recombination; RNA editing; intra-host variants; tropisms; ox-idative damage; Wuhan

Online: 6 July 2022 (15:27:32 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.1200.v1

On Mast Cells as Protectors of Life, Reproduction, and Progeny

Klas Carl Norrby

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Mast cells; Sentinel cell; Health; Ovulation; Pregnancy; Inflammation; Wound healing; Cell prolif-eration; Angiogenesis; bFGF; VEGF-A; Tissue remodeling; Histamine; Serotonin; Heparin; Proteases; IL-1-alpha; IL-8; TNF-alpha; Nitric oxide; Survival; Progeny

Online: 20 March 2024 (09:50:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1058.v1

Mesothelioma Associated Fibroblasts Modulate the Response of Mesothelioma Patient Derived Organoids to Chemotherapy through Interleukin-6.

Mario Cioce, Veronica Gatti, Fabiana Napolitano, Noemi Maria Giorgiano, Andrea Marra, Giuseppe Portella, Alfonso Fiorelli, Francesca Pentimalli, Vito Michele Fazio

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: Mesothelioma patient-derived organoids, PDO, IL-6, mesothelioma-associated fibroblasts; cocultures, cisplatin; pemetrexed; chemoresistance

Online: 18 March 2024 (15:17:01 CET)

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Background. Malignant pleural mesothelioma (MPM) is still a rather incurable disease. Many classes of compounds with various mechanisms of action have been tested in vivo and ex-vivo, with rather disappointing results. This is partly due to preclinical studies mostly performed in models that fail to recapitulate MPM complexity and do not retain the intra-tumor heterogeneity, a propelling force for tumor progression. Additionally, we and others have shown that, under stress-induced chemotherapy, profound rearrangements take place in MPM cultures to fuel the emergence of chemoresistant cell subpopulations. Thus, finding clinically actionable modulators of MPM resistance is still a very urgent need. Methods. Setup and propagation of patient-derived organoid cultures from MPM pleural effusions; flow cytometry-based validation of organoid cultures, isola-tion and propagation of mesothelioma-associated fibroblasts; co-cultures, Lu-minex-based Cytokine array, ELISA assay to quantitate IL-6 in the conditioned media, qRT-PCR to assess mRNA expression of cancer stem cell markers and fibroblast markers. Results. Organoid cultures were obtained from eight MPM patients by using specific growth media and conditions to propagate pleural effusion-derived cells. We proved that primary MPM specimens and serially passaged (mesothelio-ma-Patient-Derived-Organoids) mPDOs were cytologically similar, with the obtained mPDOs expressing mesothelial markers similarly to the originating specimen. As expected from a clinically relevant model, the size and number of mPDOs were altered, in a patient-specific way, when pharmacologically relevant doses of chemotherapy drugs were added. We derived Mesothelio-ma-Associated-Fibroblasts (MAFs) and the addition of MAFs, to various ratios, altered the response of the mPDOs to pemetrexed + cisplatin, toward a reduced sensitivity to the latter anticancer agents. Additionally, organoid formation and size were altered by MAF-conditioned media, and this correlated with increased expression of cancer stem cell markers. We showed increased levels of IL-6 in the media of the treated cocultures and showed that this latter was derived from the MAFs. Conclusions. Increased secretion of IL-6 by mesothelioma-associated fibroblasts (MAFs) contributes to the resistance of the MPM to therapy.

Preprint REVIEW | doi:10.20944/preprints202402.1548.v1

Exploring the Landscape of Anti-Inflammatory Trials: A Comprehensive Review of Strategies for Targeting Inflammation in Acute Myocardial Infraction

Andreas Mitsis, Michaela Kyriakou, Stefanos Sokratous, Georgia Karmioti, Michail Drakomathioulakis, Michael Myrianthefs, Antonios Ziakas, Stergios Tzikas, George Kassimis

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: Acute myocardial infarction; anti-inflammatory interventions; canakinumab; colchicine; coronary artery disease; IL-6; inflammation

Online: 27 February 2024 (11:52:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202310.0479.v1

Trehalose-6-Phosphate Synthase Contributes to Rapid Cold Hardening in Invasive Insect Lissorhoptrus oryzophilus (Coleoptera: Curculionidae) by Regulating Trehalose Metabolism

Juhong Zhang, Lizhong Qi, Baoyu Chen, Hongye Li, Lianglin Hu, Qingtai Wang, Shang Wang, Jinghui Xi

Subject: Biology And Life Sciences, Insect Science Keywords: trehalose-6-phosphate synthase; rapid cold hardening; trehalose metabolism; cold resistance; rice water weevil

Online: 9 October 2023 (11:16:47 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0591.v1

Bronchopulmonary Dysplasia: Pathogenesis and Pathophysiology

Nilesh Dankhara, Ira Holla, Sumana Ramarao, And Renjithkumar Kalikkot Thekkeveedu

Subject: Medicine And Pharmacology, Pediatrics, Perinatology And Child Health Keywords: 1. Bronchopulmonary dysplasia; 2. Sepsis; 3. Mechanical ventilation; 4. Pathogenesis; 5. Inflammation; 6. PDA

Online: 9 May 2023 (08:03:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0482.v1

Identification and Characterisation of Nontuberculous Mycobacteria that may Impede the Diagnosis of Bovine Tuberculosis in African Buffaloes (Syncerus caffer), South Africa

Charlene Clarke, Tanya J. Kerr, Robin M. Warren, Léanie Kleynhans, Michele Ann Miller, Wynand Johan Goosen

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: African buffaloes; bovine tuberculosis; cfp-10; esat-6; hsp65; nontuberculous mycobacteria; rpoB; Syncerus caffer

Online: 29 August 2022 (09:48:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0363.v1

Safety of Dietary Camelina Oil Supplementation in Healthy, Adult Dogs

Scarlett Burron, Taylor Richards, Keely Patterson, Caitlin Grant, Nadeem Akhtar, Luciano Trevizan, Wendy Pearson, and Anna Kate Shoveller

Subject: Biology And Life Sciences, Food Science And Technology Keywords: omega-3; omega-6; fatty acids; camelina oil, flaxseed oil; canola oil; canine nutrition

Online: 15 July 2021 (18:17:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0038.v1

Diabetes Induced Renal Complications by Leukocyte Activation of Nuclear Factor κ-B and its Regulated Genes Expression

Noura Darwish, Yousif Elnahas, Fatmah AlQahtany

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: NF-κB; IL-1β; IL-6; VEGF; TNF-α; FN; ICAM-1; VCAM-1

Online: 2 September 2020 (09:46:10 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202004.0143.v1

Inflamm-Aging: Why Older Men Are the Most Susceptible to SARS-Cov-2 Complicated Outcomes

Massimiliano Bonafè, Francesco Prattichizzo, Angelica Giuliani, Gianluca Storci, Jacopo Sabbatinelli, Fabiola Olivieri

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: SARS-CoV-2; COVID-19; IL-6; inflamm-aging; immune senescence; host-directed therapies

Online: 9 April 2020 (08:38:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints201902.0245.v1

Updates on the CDK4/6 Inhibitory Strategy and Combinations in Breast Cancer

Navid Sobhani, Alberto D'Angelo, Matteo Pittacolo, Giandomenico Roviello, Tobias Otto

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: cyclin-dependent kinases; cyclin-dependent kinase 4 and 6 inhibitors; targeted therapies; breast cancer

Online: 27 February 2019 (04:48:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints201804.0277.v1

Si-Disordering in MgAl₂O₄-Spinel at High P-T Conditions, with Implication to Si-Mg Disorder in Mg₂SiO₄-Ringwoodite

Liping Liu, Xi Liu, Xinjian Bao, Qiang He, Wei Yan, Yunlu Ma, Mingyue He, Renbiao Tao, Ruqiang Zou

Subject: Environmental And Earth Sciences, Geochemistry And Petrology Keywords: 4-coordinated Si; 6-coordinated Si; MgAl2O4-spinel; Mg2SiO4-ringwoodite; Raman spectroscopy; Si-disordering

Online: 23 April 2018 (08:37:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201702.0087.v1

Metal-Free α-C(sp³)–H Functionalized Oxidative Cyclization of Tertiary N,N-Diaryl Amino Alcohols: Theoretical Approach for Mechanistic Pathway

Zakir Ullah, Mihyun Kim

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: α-C(sp3)–H functionalization; oxidative cyclization; tertiary amine; 6-exo-trig; metal free

Online: 23 February 2017 (10:40:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202202.0360.v1

Effect of Kinematic Algorithm Selection on the Conceptual De-Sign of Orbitally Delivered Vehicles

David Cole, Timothy Sands

Subject: Engineering, Mechanical Engineering Keywords: GNC system design; design and analysis of orbit/attitude determination and control systems; motion planning; guidance; control; trajectory planning; conceptual design; kinematics; direction cosine matrix; DCM; 6 Degrees of Freedom; 6 DOF

Online: 28 February 2022 (12:22:36 CET)

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A particular challenge in the conceptual design of direct-from orbit delivery systems is the seemingly well-known kinematics retain fallacies whose efficacies are reduced over great distances. Rotation about the local wing of an aerospace vehicle is almost never the pitch angle, yet modern application of kinematics often assumes such (with accompanying angular error). The same assertion is usually true about the nature of roll and yaw angles. Expressing motion in coordinates of rotating reference frames necessitates transformation between reference frames, and one such transformation is embodied in the Direction Cosine Matrices (DCM) formed by a sequence of three successive frame rotations. One of two ubiquitous sequences of three rotations used to construct the DCM involves first a rotation around the inertial z-axis, then intermediate y-axis, then finally about the body’s x-axis; a sequence commonly called the “aerospace sequence” or the a “3-2-1 rotation sequence”. The second ubiquitous sequence is the so-called “orbital sequence” or “3-1-3 rotation sequence” with rotations about the inertial z axis first, then about the intermediate x-axis, then finally about the body z-axis. This manuscript evaluates which sequence is the most advantageous for an object starting in space and then travels through the atmosphere to a target on the Earth’s surface. Six degrees of freedom of vehicle motion were simulated starting in orbit with a given thrust and commanded maneuver. The simulation performs all twelve possible rotation sequences (transforming inertial coordinates to body coordinates) with comparison by computational burden and error representing rotations about body axes (roll, pitch, and yaw respectively). Simulation precision is validated using the quaternion normalization condition indicating near machine precision (0.9\times{10}^{-15}) and reveals the so-called 132 rotation is the most accurate with an average error of 0.14° and a computational time of 0.013 seconds: resulting in a 97.95% increase in accuracy over the so-called 321 rotation and a 99.84% increase over the so-called 313 rotation.

Preprint ARTICLE | doi:10.20944/preprints202401.0771.v1

Collembola Communities Reflect Soil Recovery After Fire in Mediterranean Pinus pinaster Forests

María José Luciáñez, Alicia Serena

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: Collembola; edaphic communities recovery; Alpha diversity indexes; burned forest; removal and burning of wood after fire

Online: 10 January 2024 (08:12:52 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1407.v1

Evaluation of Phα1β Interaction on the Kv11.1 Potassium Channel in HEK293 Cells Trans-fected with the Human ERG Channel

Joao Batista Calixto, Adara Aurea Santos, Juliano Ferreira, Alessandra Hubner Souza, Célio José Castro Junior, Marcus Vinicius Gomez

Subject: Biology And Life Sciences, Other Keywords: Phα 1β ; analgesic; dofetilide; Kv11.1 potassium channel; hERG channel interaction; HEK293-hERG; cells viability

Online: 22 November 2023 (09:48:35 CET)

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Preprint REVIEW | doi:10.20944/preprints202308.0519.v1

Alpha-Linolenic Acid and Cardiovascular Events: A Narrative Review

Camilla Bertoni, Martina Abodi, Veronica D’Oria, Gregorio P. Milani, Carlo Agostoni, Alessandra Mazzocchi

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: alpha-linolenic acid; hempseed oil; cardiovascular events; LDL-cholesterol; TCcholesterol; cardioprotective effect; heart disorders; linoleic acid

Online: 7 August 2023 (11:37:39 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.2029.v1

Inflammatory Cytokines in Psoriatic Arthritis: Understanding Pathogenesis and Implications for Treatment

Bong-Woo Lee, Su-Jin Moon

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: psoriatic arthritis; inflammatory cytokines; tumor necrosis factor-alpha; interleukin-17; interleukin-23; JAK/STAT signaling pathway

Online: 28 June 2023 (12:30:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0295.v1

Multi-criterion Sampling Matting Algorithm via Gaussian Process

Yuan Yang, Hong shan Gou, Mian Tan, Fu Jian Feng, Yi hui Liang, Yi Xiang, Lin Wang, Han Huang

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: computing resources; Gaussian process fitting model; multi-criterion sampling strategy; high-quality pixel pairs; alpha matte

Online: 5 June 2023 (10:00:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0410.v1

Treated Wastewater Assessment to Optimize Agricultural Water Reuse in Al-Qatif Region Saudi Arabia Using Hybrid Machine Learning Techniques

Syed Muzzamil Hussain Shah, Mohamed A. Yassin, Sani I. Abba, Dahiru U. Lawal, Farouq Aliyu, Ebrahim Hamid Hussain Al-Qadami, Zahiraniza Mustaffa, Chaitanya B. Pande, Saad Sh. Sammen, Isam H. Aljundi

Subject: Environmental And Earth Sciences, Water Science And Technology Keywords: electrical conductivity; treated waste water; machine learning; prediction analysis; agricultural irrigation; water security; SDG-6

Online: 7 March 2024 (07:53:10 CET)

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In the era of mounting water scarcity, particularly in arid regions, the agricultural sector is striving to optimize the water resource management practices. Treated wastewater (TWW) has emerged as a viable alternative to freshwater for irrigation, offering a sustainable solution to water conservation. However, ensuring the safety and quality of TWW is paramount to safeguard for both crop health and environmental integrity. Electrical conductivity (EC), a critical factor in assessing TWW suitability for irrigation, can significantly impact crop yields and soil health since it is directly linked to the concentration of dissolved salts and ions. Therefore, this study delves into the development of advanced predictive models for EC assessment by employing supervised machine learning (ML) techniques like the Support Vector Regression (SVR), Adaptive Network-based Fuzzy Inference System (ANFIS), and Multiple Linear Regression (MLR). The TWW samples collected from Al-Qatif area, Saudi Arabia were assessed in the lab through a sensor-based real-time monitoring system integrated with Internet of Things (IoT) network and a multiprobe device. The key water quality parameters assessed involved, temperature (T), pH, Oxidation Reduction Potential (ORP), Resistivity (RES), Total Dissolved Solids (TDS), Salinity and Turbidity which served as the foundation for these models, given their profound influence on and their anticipated impact on EC levels. Following feature selection, two distinct model combinations were identified for the SVR, ANFIS and MLR models. Furthermore, to evaluate prediction performance, four statistical criteria, including coefficient of determination (R²), correlation coefficient (R), mean square error (MSE), and root mean square error (RMSE), were employed. Overall, the comparative performance results proved that ANFIS-M2 indicated the strongest predictive capability with the R² value of 0.99572 and 0.75145 and the RMSE values of 0.00965 and 0.01134, respectively in both calibration and verification stages.

Preprint ARTICLE | doi:10.20944/preprints202402.1420.v1

Characterization and Simulation of the Interface between a Continuous and Discontinuous Fiber Reinforced Thermoplastic by Using the Climbing Drum Peel Test Considering Humidity

Nicolas Christ, Benedikt M. Scheuring, Christoph Schelleis, Wilfried V. Liebig, John Montesano, Kay A. Weidenmann, Jörg Hohe

Subject: Chemistry And Materials Science, Polymers And Plastics Keywords: Carbon fibers; Polyamide 6; Layered structures; Delamination; Cohesive interface modeling; Mechanical testing; Continuous-Discontinuous FRP

Online: 26 February 2024 (11:46:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1819.v1

Self-Consistent Solution of Schrödinger-Poisson Equations For Modeling Realistic Silicon Transistors

Srinjoy Sen

Subject: Engineering, Electrical And Electronic Engineering Keywords: quantum transport; QTBM; Schrodinger equation; Poisson equation; Device Physics; 6 Solid State Physics

Online: 25 January 2024 (10:06:20 CET)

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Preprint REVIEW | doi:10.20944/preprints202308.0453.v1

In Vivo Gene Editing: A Healthcare Revolution in the Making

Sarfaraz K. Niazi

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: gene editing 1; CRISPR 2; gRNA 3; autoimmune disorders 4; nuclease 5; hereditary disorders 6

Online: 4 August 2023 (19:15:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1004.v1

Cuban Policosanol (Raydel®) Exhibited Potent Anti-inflammatory Activity in the Liver and Protection of the Ovary and Testis with an Improvement of Blood Lipid Profile by 12 Weeks Supplementation in Hyperlipidemic Zebrafish: Comparison Study of Efficacy with Three Chinese Policosanols

Kyung-Hyun Cho, Ji-Eun Kim, Seung Hee Baek

Subject: Biology And Life Sciences, Life Sciences Keywords: Policosanol; high cholesterol diet; high-density lipoproteins; apoA-I; inflammation; interleukin-6; liver; testis; ovary

Online: 14 July 2023 (09:27:04 CEST)

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Although many policosanols from different sources, such as sugar cane, rice bran, and insects, have been marketed worldwide, there has been no comparative study of the in vivo efficacy using a hyperlipidemic animal model. This study compared the efficacy of treating dyslipidemia with four different policosanols, including one sugar cane wax alcohol from Cuba (Raydel®) and three policosanols from China (Xi’an Natural sugar cane, Xi’an Realin sugar cane, and Shaanxi rice bran) in hyperlipidemic zebrafish. After 12 weeks of consumption of each policosanol (final 0.1% in diet, wt/wt) and high cholesterol diet (HCD, final 4%, wt/wt) in a tetrabit, the Raydel policosanol group and Xi’an Natural policosanol group showed the highest survivability of approximately 81%, whereas the Xi’an Realin policosanol group and Shaanxi policosanol group showed 57% and 67% survivability, respectively. Among five HCD groups, the Raydel policosanol group showed the lowest serum total cholesterol (TC, p<0.001 versus HCD control) and triglyceride (TG, p<0.001 versus HCD control) with the highest percentage of high-density lipoproteins (HDL)-C in TC. The Raydel policosanol group also showed the lowest serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels with the least infiltration of inflammatory cells and interleukin-6 production in hepatocytes. In the ovary, the Raydel policosanol group also showed the highest contents of mature vitellogenic oocytes with the lowest production of reactive oxygen species and cellular apoptosis in ovarian cells. In the testis, the Raydel policosanol group also showed the healthiest morphology for spermatogenesis with the least interstitial area and reactive oxygen species (ROS) production in testicular cells. In conclusion, 12 weeks of Raydel policosanol consumption resulted in remarkable improvement of the blood lipid profile, amelioration of inflammation in the liver, and enhancement of the cell morphology in the ovary and testis with the highest survivability under the HCD.

Preprint ARTICLE | doi:10.20944/preprints202307.0881.v1

Emotional Distress in Portuguese Cancer Patients: The Use of the Emotion Thermometers

Sónia Silva, Tiago Paredes, Ricardo João Teixeira, Tânia Brandão, Klára Dimitrovová, Diogo Marques, Joana Sousa, Monick Leal, Albina Dias, Carole Neves, Graciete Marques, Natália Amaral

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: distress thermometer 1; emotion thermometer 2; emotional distress 3; anxiety 4; depression 5; cancer 6

Online: 13 July 2023 (09:56:02 CEST)

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