Version 1
: Received: 24 November 2023 / Approved: 24 November 2023 / Online: 24 November 2023 (09:31:34 CET)
How to cite:
Halloran, D.; Pandit, V.; Chukwuocha, K.; Nohe, A. Methyl-Beta-Cyclodextrin Restores Aberrant Bone Morphogenetic Protein 2-Signaling in Bone Marrow Stromal Cells Obtained from Aged C57BL/6 Mice. Preprints2023, 2023111592. https://doi.org/10.20944/preprints202311.1592.v1
Halloran, D.; Pandit, V.; Chukwuocha, K.; Nohe, A. Methyl-Beta-Cyclodextrin Restores Aberrant Bone Morphogenetic Protein 2-Signaling in Bone Marrow Stromal Cells Obtained from Aged C57BL/6 Mice. Preprints 2023, 2023111592. https://doi.org/10.20944/preprints202311.1592.v1
Halloran, D.; Pandit, V.; Chukwuocha, K.; Nohe, A. Methyl-Beta-Cyclodextrin Restores Aberrant Bone Morphogenetic Protein 2-Signaling in Bone Marrow Stromal Cells Obtained from Aged C57BL/6 Mice. Preprints2023, 2023111592. https://doi.org/10.20944/preprints202311.1592.v1
APA Style
Halloran, D., Pandit, V., Chukwuocha, K., & Nohe, A. (2023). Methyl-Beta-Cyclodextrin Restores Aberrant Bone Morphogenetic Protein 2-Signaling in Bone Marrow Stromal Cells Obtained from Aged C57BL/6 Mice. Preprints. https://doi.org/10.20944/preprints202311.1592.v1
Chicago/Turabian Style
Halloran, D., Kelechi Chukwuocha and Anja Nohe. 2023 "Methyl-Beta-Cyclodextrin Restores Aberrant Bone Morphogenetic Protein 2-Signaling in Bone Marrow Stromal Cells Obtained from Aged C57BL/6 Mice" Preprints. https://doi.org/10.20944/preprints202311.1592.v1
Abstract
As humans age, aberrancies in several signaling pathways occur. In the aging population, some patients display abnormal bone morphogenetic protein (BMP) signaling. This can lead to osteoporosis (OP), a debilitating bone disorder caused by an imbalance between osteoblasts and osteoclasts. In 2002, the Food and Drug Administration (FDA) approved usage of recombinant human BMP-2 (rhBMP-2) during spinal fusion surgery as it is crucial for bone formation. However, complications have been reported with rhBMP-2 and primary osteoblasts isolated from OP patients are unresponsive to BMP-2. While patient samples are available, previous medical history may alter results. Therefore, C57BL/6 mice, an aging model that displays aberrant BMP-signaling, can be utilized to investigate OP and aging. We show that BMP receptor type Ia (BMPRIa) is upregulated in the bone marrow stromal cells (BMSCs) of 15-month mice similar to previous data. We show that a conjugation between BMP-2 and Quantum Dots (QDot®s) effectively binds to BMPRIa and can investigate BMP-2 activity. After incubating BMSCs with methyl-β-cyclodextrin (MβCD), BMP-signaling is restored in 15-month mice as shown by western blotting and the von Kossa assay. MβCD may be used to rescue BMPRIa and the BMP-signaling pathway can be utilized by future therapeutics to treat OP.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
