Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Low Levels of IgG Recognizing the Alpha-1-Antitrypsin (A1AT) 50-63 Peptide and Its Association with Taiwanese Women with Primary Sjögren's Syndrome

Yu-Sheng Chang
† ,
Chih-Hong Pan
,
Che-Chang Chang
† ORCID logo ,
Kai-Leun Tsai
,
Han-Wen Chou
,
Jin-Hua Chen
,
Sheng-Hong Lin
,
Yi-Ying Lu
,
Chih-Chun Tai
,
Yi-Fang Lin
,
Ching-Yu Lin
* ORCID logo
†
These authors contributed equally to this paper
Version 1 : Received: 28 October 2017 / Approved: 30 October 2017 / Online: 30 October 2017 (04:30:59 CET)

A peer-reviewed article of this Preprint also exists.

Chang, Y.-S.; Pan, C.-H.; Chang, C.-C.; Tsai, K.-L.; Chou, H.-W.; Chen, J.-H.; Lin, S.-H.; Lu, Y.-Y.; Tai, C.-C.; Lin, Y.-F.; Lin, C.-Y. Low Levels of IgG Recognizing the α-1-Antitrypsin Peptide and Its Association with Taiwanese Women with Primary Sjögren’s Syndrome. Int. J. Mol. Sci. 2017, 18, 2750. Chang, Y.-S.; Pan, C.-H.; Chang, C.-C.; Tsai, K.-L.; Chou, H.-W.; Chen, J.-H.; Lin, S.-H.; Lu, Y.-Y.; Tai, C.-C.; Lin, Y.-F.; Lin, C.-Y. Low Levels of IgG Recognizing the α-1-Antitrypsin Peptide and Its Association with Taiwanese Women with Primary Sjögren’s Syndrome. Int. J. Mol. Sci. 2017, 18, 2750.

Abstract

The aim of this study was to examine oxidative stress and low level of alpha-1-antitrypsin (A1AT) in primary Sjögren's syndrome (pSS), and evaluate the associated autoreactivity against unmodified and their 4-hydroxy-2-nonenal (HNE)-modified peptides with pSS. Two differentially expressed proteins, alpha-1-acid glycoprotein 1 (A1AG1) and A1AT, exhibited 2-fold differences, and their HNE modifications were identified by depleted-albumin and immunoglobulin G (IgG) serum protein, in-solution digestion, in-gel digestion, and nano-LC-MS/MS from pSS patients and age-matched healthy controls (HCs). Furthermore, levels of proteins, confirmation of HNE modifications, HNE-protein adducts and autoreactivity against unmodified and their HNE-modified peptides were further validated. Levels of the HNE-protein adduct and A1AG1 were significantly higher in pSS patients than HCs, but levels of A1AT were significantly lower in pSS patients compared to HCs. Only the HNE modification of A1AT was confirmed. Further, concentrations of anti-A1AT50-63 IgG and anti-A1AT50-63 HNE IgA were significantly lower in pSS patients than HCs. Our study suggests that elevated HNE-protein adduct, oxidative stress, level [odds ratio (OR) 4.877, p = 0.003], lowered A1AT level (OR 3.910, p = 0.010) and a decreased level of anti-A1AT50-63 IgG (OR 3.360, p = 0.010) showed an increased risk in pSS patients compared to HCs, respectively.

Keywords

primary Sjögren's syndrome; alpha-1-antitrypsin; inhibitor; 4-hydroxy-2-nonenal; autoantibody isotypes; serum

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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